Shop All Avidin, Streptavidin & Biotin
Streptavidin, (HRPO) (Invitrogen™)
• Class: RUO
• Statement: For research use only. Not for use in diagnostic procedures.
• Buffer: PBS
• Statement: For research use only. Not for use in diagnostic procedures.
• Buffer: PBS
Streptavidin, (Alk. Phos.) (Invitrogen™)
• Class: RUO
• Statement: For research use only. Not for use in diagnostic procedures.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Statement: For research use only. Not for use in diagnostic procedures.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
Qdot™ 625 Streptavidin Conjugate (Invitrogen™)
Qdot® 625 Streptavidin Conjugate comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Qdot® nanocrystal). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays, and are also used in purification workflows to achieve targeted fractionation. Qdot® nanocrystal conjugates are provided as 1 µM solutions.
Important Features of Qdot® Streptavidin Conjugates:
• Streptavidin Qdot® 625 conjugate has emission maxima of ~625 nm
• Approximately 5 to 10 streptavidins per Qdot® nanocrystal
• Extremely photostable and bright fluorescence
• Efficiently excited with single-line excitation sources
• Narrow emission, large Stokes shift
• Available in multiple colors
• Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more
Properties of Qdot® Nanocrystals
The Qdot® streptavidin conjugate is the size of a large macromolecule or protein (~15–20 nm) and represents the brightest class of streptavidin detection reagents. Qdot® streptavidin conjugates are made from a nanometer-scale crystal of a semiconductor material (CdSe), which is coated with an additional semiconductor shell (ZnS) to improve the optical properties of the material. The Qdot® 705 and Qdot® 800 streptavidin conjugates, which include CdSeTe, are made in a similar fashion. This core-shell material is further coated with a polymer shell that allows the materials to be conjugated to biological molecules and to retain their optical properties.
Other Fluorescent Conjugates of Streptavidin are Available
We offer several other Qdot® colors or try the Qdot® Streptavidin Sampler Kit, containing Qdot® streptavidin conjugates in six colors 525, 565, 585, 605, 655, and 705. In addition to nanocrystal conjugates, we offer a wide range of streptavidins conjugated to Alexa Fluor® dyes, Oregon Green® dye, enzyme conjugates, and traditional fluorophores like Texas Red® dye, fluorescein (FITC) and more.
Find Biotinylated Conjugates
We offer an extensive line of biotinylated conjugates for use in biotin-streptavidin detection strategies.
• Use the primary antibody search tool to find biotinylated primary antibodies
• Use the secondary antibody selector tool to find biotinylated secondary antibodies and biotinylated anti-dye and anti-hapten antibodies
Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.
Related Links:
Learn more about Avidin-Biotin Detection
Learn more about Qdot® Nanocrystals
Important Features of Qdot® Streptavidin Conjugates:
• Streptavidin Qdot® 625 conjugate has emission maxima of ~625 nm
• Approximately 5 to 10 streptavidins per Qdot® nanocrystal
• Extremely photostable and bright fluorescence
• Efficiently excited with single-line excitation sources
• Narrow emission, large Stokes shift
• Available in multiple colors
• Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more
Properties of Qdot® Nanocrystals
The Qdot® streptavidin conjugate is the size of a large macromolecule or protein (~15–20 nm) and represents the brightest class of streptavidin detection reagents. Qdot® streptavidin conjugates are made from a nanometer-scale crystal of a semiconductor material (CdSe), which is coated with an additional semiconductor shell (ZnS) to improve the optical properties of the material. The Qdot® 705 and Qdot® 800 streptavidin conjugates, which include CdSeTe, are made in a similar fashion. This core-shell material is further coated with a polymer shell that allows the materials to be conjugated to biological molecules and to retain their optical properties.
Other Fluorescent Conjugates of Streptavidin are Available
We offer several other Qdot® colors or try the Qdot® Streptavidin Sampler Kit, containing Qdot® streptavidin conjugates in six colors 525, 565, 585, 605, 655, and 705. In addition to nanocrystal conjugates, we offer a wide range of streptavidins conjugated to Alexa Fluor® dyes, Oregon Green® dye, enzyme conjugates, and traditional fluorophores like Texas Red® dye, fluorescein (FITC) and more.
Find Biotinylated Conjugates
We offer an extensive line of biotinylated conjugates for use in biotin-streptavidin detection strategies.
• Use the primary antibody search tool to find biotinylated primary antibodies
• Use the secondary antibody selector tool to find biotinylated secondary antibodies and biotinylated anti-dye and anti-hapten antibodies
Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.
Related Links:
Learn more about Avidin-Biotin Detection
Learn more about Qdot® Nanocrystals
LanthaScreen™ Eu-Streptavidin
A general reagent for use in assay development, the Europium-Streptavidin binds to biotinylated peptide or physiological protein substrates.
Streptavidin, (Cy3) (Invitrogen™)
• Class: RUO
• Statement: For research use only. Not for use in diagnostic procedures.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
View a selection guide for all Alexa Fluor® streptavidin and avidin conjugates.
• Statement: For research use only. Not for use in diagnostic procedures.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
View a selection guide for all Alexa Fluor® streptavidin and avidin conjugates.
Streptavidin, (Cy5) (Invitrogen™)
• Class: RUO
• Statement: For research use only. Not for use in diagnostic procedures.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
View a selection guide for all Alexa Fluor® streptavidin and avidin conjugates.
• Statement: For research use only. Not for use in diagnostic procedures.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
View a selection guide for all Alexa Fluor® streptavidin and avidin conjugates.
eBioscience™ Avidin HRP (Invitrogen™)
L’enzyme eBioscience™ Avidin HRP (peroxydase de raifort) est couramment utilisée lors de la deuxième étape de détection des anticorps biotinylés par test ELISA et Western Blot. L’avidine se lie à la biotine avec une affinité de liaison élevée. [Remarque : l’azoture de sodium est un inhibiteur de l’activité enzymatique de la peroxydase de raifort et il ne doit pas être ajouté au diluant AV-HRP diluent.]
Alexa Fluor™ 488 Streptavidin, 10 nm colloidal gold conjugate (Invitrogen™)
Alexa Fluor® 488 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor® dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays. Alexa Fluor® dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.
Important Features of Alexa Fluor® 488 Streptavidin Conjugates:
• Alexa Fluor® 488 streptavidin conjugate has Ex/Em maxima of ~ (495/519)
• Bright, photostable fluorescence
• High solubility in aqueous solutions
• Available in multiple colors
• Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more
Properties of Alexa Fluor® Dyes
Alexa Fluor® dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.
Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Related Links:
Learn more about Avidin-Biotin Detection
Learn more about Alexa Fluor® Dyes
Find out about other Labeled Streptavidin Conjugates
Read Avidin and Streptavidin Conjugates-Section 7.6 in the Molecular Probes Handbook
Important Features of Alexa Fluor® 488 Streptavidin Conjugates:
• Alexa Fluor® 488 streptavidin conjugate has Ex/Em maxima of ~ (495/519)
• Bright, photostable fluorescence
• High solubility in aqueous solutions
• Available in multiple colors
• Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more
Properties of Alexa Fluor® Dyes
Alexa Fluor® dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.
Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Related Links:
Learn more about Avidin-Biotin Detection
Learn more about Alexa Fluor® Dyes
Find out about other Labeled Streptavidin Conjugates
Read Avidin and Streptavidin Conjugates-Section 7.6 in the Molecular Probes Handbook
Streptavidin, (PE-Cy7) (Invitrogen™)
• Class: RUO
• Statement: For research use only. Not for use in diagnostic procedures.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
View a selection guide for all streptavidin RPE, APC, and tandem conjugates.
• Statement: For research use only. Not for use in diagnostic procedures.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
View a selection guide for all streptavidin RPE, APC, and tandem conjugates.
Qdot™ 605 Streptavidin Conjugate (Invitrogen™)
Qdot® 605 streptavidin conjugate comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Qdot® nanocrystal). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays, and are also used in purification workflows to achieve targeted fractionation. Qdot® nanocrystal conjugates are provided as 1 µM solutions.
Important Features of Qdot® Streptavidin Conjugates:
• Streptavidin Qdot® 605 conjugate has emission maxima of ~605 nm
• Approximately 5 to 10 streptavidins per Qdot® nanocrystal
• Extremely photostable and bright fluorescence
• Efficiently excited with single-line excitation sources
• Narrow emission, large Stokes shift
• Available in multiple colors
• Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more
Properties of Qdot® Nanocrystals
The Qdot® streptavidin conjugate is the size of a large macromolecule or protein (~15–20 nm) and represents the brightest class of streptavidin detection reagents. Qdot® streptavidin conjugates are made from a nanometer-scale crystal of a semiconductor material (CdSe), which is coated with an additional semiconductor shell (ZnS) to improve the optical properties of the material. The Qdot® 705 and Qdot® 800 streptavidin conjugates, which include CdSeTe, are made in a similar fashion. This core-shell material is further coated with a polymer shell that allows the materials to be conjugated to biological molecules and to retain their optical properties.
Other Fluorescent Conjugates of Streptavidin are Available
We offer several other Qdot® colors or try the Qdot® Streptavidin Sampler Kit, containing Qdot® streptavidin conjugates in six colors 525, 565, 585, 605, 655, and 705. In addition to nanocrystal conjugates, we offer a wide range of streptavidins conjugated to Alexa Fluor® dyes, Oregon Green® dye, enzyme conjugates, and traditional fluorophores like Texas Red® dye, fluorescein (FITC) and more.
Find Biotinylated Conjugates
We offer an extensive line of biotinylated conjugates for use in biotin-streptavidin detection strategies.
• Use the primary antibody search tool to find biotinylated primary antibodies
• Use the secondary antibody selector tool to find biotinylated secondary antibodies and biotinylated anti-dye and anti-hapten antibodies
Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Related Links:
Learn more about Avidin-Biotin Detection
To learn more about Qdot® Nanocrystals
Important Features of Qdot® Streptavidin Conjugates:
• Streptavidin Qdot® 605 conjugate has emission maxima of ~605 nm
• Approximately 5 to 10 streptavidins per Qdot® nanocrystal
• Extremely photostable and bright fluorescence
• Efficiently excited with single-line excitation sources
• Narrow emission, large Stokes shift
• Available in multiple colors
• Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more
Properties of Qdot® Nanocrystals
The Qdot® streptavidin conjugate is the size of a large macromolecule or protein (~15–20 nm) and represents the brightest class of streptavidin detection reagents. Qdot® streptavidin conjugates are made from a nanometer-scale crystal of a semiconductor material (CdSe), which is coated with an additional semiconductor shell (ZnS) to improve the optical properties of the material. The Qdot® 705 and Qdot® 800 streptavidin conjugates, which include CdSeTe, are made in a similar fashion. This core-shell material is further coated with a polymer shell that allows the materials to be conjugated to biological molecules and to retain their optical properties.
Other Fluorescent Conjugates of Streptavidin are Available
We offer several other Qdot® colors or try the Qdot® Streptavidin Sampler Kit, containing Qdot® streptavidin conjugates in six colors 525, 565, 585, 605, 655, and 705. In addition to nanocrystal conjugates, we offer a wide range of streptavidins conjugated to Alexa Fluor® dyes, Oregon Green® dye, enzyme conjugates, and traditional fluorophores like Texas Red® dye, fluorescein (FITC) and more.
Find Biotinylated Conjugates
We offer an extensive line of biotinylated conjugates for use in biotin-streptavidin detection strategies.
• Use the primary antibody search tool to find biotinylated primary antibodies
• Use the secondary antibody selector tool to find biotinylated secondary antibodies and biotinylated anti-dye and anti-hapten antibodies
Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Related Links:
Learn more about Avidin-Biotin Detection
To learn more about Qdot® Nanocrystals
Streptavidin, R-Phycoerythrin Conjugate (SAPE) - premium grade - 1 mg/mL (Invitrogen™)
The R-phycoerythrin (R-PE) conjugate of streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (R-phycoerythrin). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Streptavidin R-PE is very valuable in biotin-streptavidin detection protocols developed for flow cytometry, microplate assays, and microarrays. This R-PE streptavidin conjugate is a high-purity product (purity confirmed by HPLC) and is supplied as 1 mL of a 1 mg/mL solution.
Important Features of Streptavidin RPE:
• Ex/Em maxima of ~ (496, 546, 565/578) nm
• 5 to 10 times brighter than organic dyes
• Very high solubility in aqueous solutions
• Free of all unconjugated streptavidin; and mostly free of unconjugated R-PE
• Ideal for flow cytometry, microplate assays, and microarrays
Properties of R-phycoerythrin
R-PE has a quantum efficiency of 78%, making it an incredibly bright reagent, useful where high sensitivity is needed. Because of poor photostability, however, it is not recommended for fluorescence microscopy.
Find Biotinylated Conjugates
We offer an extensive line of biotinylated conjugates for use in biotin-streptavidin detection strategies.
• Use the primary antibody search tool to find biotinylated primary antibodies
• Use the secondary antibody selector tool to find biotinylated secondary antibodies and biotinylated anti-dye and anti-hapten antibodies
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Related Links:
Learn more about Avidin-Biotin Detection
Learn more about the practical applications for Streptavidin R-PE
Important Features of Streptavidin RPE:
• Ex/Em maxima of ~ (496, 546, 565/578) nm
• 5 to 10 times brighter than organic dyes
• Very high solubility in aqueous solutions
• Free of all unconjugated streptavidin; and mostly free of unconjugated R-PE
• Ideal for flow cytometry, microplate assays, and microarrays
Properties of R-phycoerythrin
R-PE has a quantum efficiency of 78%, making it an incredibly bright reagent, useful where high sensitivity is needed. Because of poor photostability, however, it is not recommended for fluorescence microscopy.
Find Biotinylated Conjugates
We offer an extensive line of biotinylated conjugates for use in biotin-streptavidin detection strategies.
• Use the primary antibody search tool to find biotinylated primary antibodies
• Use the secondary antibody selector tool to find biotinylated secondary antibodies and biotinylated anti-dye and anti-hapten antibodies
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Related Links:
Learn more about Avidin-Biotin Detection
Learn more about the practical applications for Streptavidin R-PE
eBioscience™ Streptavidin PE (Invitrogen™)
Les conjugués streptavidine-fluorochrome sont couramment utilisés dans les protocoles de coloration indirecte pour détecter les anticorps primaires biotinylés dans les applications de cytométrie de flux. La streptavidine se lie à la biotine avec une affinité de liaison élevée.
LanthaScreen™ Eu-Streptavidin Kit
A general reagent for use in assay development, the Europium-Streptavidin binds to biotinylated peptide or physiological protein substrates. The Eu-streptavidin Kit contains 10 µg Eu-Streptavidin, 400 µl AlexaFluor® 647-biotin control peptide, and 100 mL of TR-FRET Dilution Buffer.
