Shop All Stem Cells
StemPro™ Human Adipose-Derived Stem Cells (Gibco™)
Human Adipose Derived Stem Cells (ADSCs) are isolated from human lipoaspirate tissue and cryopreserved from primary cultures. Before being cryopreserved the ADSCs are expanded for one passage in MesenPRO RS™ Medium (Cat. no. 12746-012) - a revolutionary new reduced serum (2%) medium that results in reduced ADSC doubling times. Each lot of ADSCs originates from a single donor of human lipoaspirate tissue. Each vial of ADSCs contains cells that can differentiate into multiple mature cell phenotypes in vitro including adipocytes, osteoblasts and chondrocytes. ADSCs have demonstrated very similar phenotypic and functional characteristics to that of bone marrow-derived mesenchymal stem cells. The ADSCs express a flow cytometry cell surface protein profile as follows: positive CD29, CD44, CD73, CD90, CD105, CD166; negative CD14, CD31, CD45, Lin1. For optimal ADSC growth and expansion, MesenPRO RS™ Medium is recommended.
BG01V/hOG Human Embryonic Stem Cells (hESCs) (Gibco™)
Variant hESC hOct4-GFP Reporter Cells
BG01V/hOG Cells are derived from the BG01V human embryonic stem cell (hESC) line (ATCC No. SCRC-2002) (Mitalipova et al. (2003); Plaia et al. (2006)). BG01V cells stain positively for pluripotency markers and can differentiate to representatives of all three primary germ layers. An integration vector containing an EmGFP (Emerald Green Fluorescent Protein) gene directed by the human Oct4 promoter and followed by the HSV-TK polyadenylation signal was stably integrated into the genome of BG01V cells. The engineered cells express EmGFP under the appropriate conditions when the Oct4 promoter is active. This promoter is only active when hESCs are in the pluripotent state and therefore acts as a sensitive indicator of differentiation (Pan et al. (2002)).
Applications:
1. BG01V/hOG Cells are engineered to enable monitoring of pluripotency of hESCs without sacrificing cells. When pluripotent, these cells express EmGFP. EmGFP expression is lost upon differentiation.
2. BG01V/hOG Cells can be used as a reporter of the culture’s response to external stimuli including media composition, stress, and whether or not culture conditions are adequate to maintain hESCs in an undifferentiated state.
3. BG01V/hOG Cells can be used to determine the efficiency of your differentiation assays.
Intended use:
Research use only. Caution: Not intended for human or animal diagnostic or therapeutic uses.
Contents and Storage:
The vial of BG01V/hOG Cells should be stored in liquid nitrogen.
References:
1. Mitalipova, M. et al. (2003) Stem Cells 21: 521-526
2. Pan, G. J. et al. (2002) Cell Res 12: 321-329
3. Plaia, T. W. et al. (2006) Stem Cells 24: 531-546
Useful links:
User Manual for BG01V/hOG Cells
Embryonic Stem Cell (ESC) Product Overview Sheet
BG01V/hOG Cells are derived from the BG01V human embryonic stem cell (hESC) line (ATCC No. SCRC-2002) (Mitalipova et al. (2003); Plaia et al. (2006)). BG01V cells stain positively for pluripotency markers and can differentiate to representatives of all three primary germ layers. An integration vector containing an EmGFP (Emerald Green Fluorescent Protein) gene directed by the human Oct4 promoter and followed by the HSV-TK polyadenylation signal was stably integrated into the genome of BG01V cells. The engineered cells express EmGFP under the appropriate conditions when the Oct4 promoter is active. This promoter is only active when hESCs are in the pluripotent state and therefore acts as a sensitive indicator of differentiation (Pan et al. (2002)).
Applications:
1. BG01V/hOG Cells are engineered to enable monitoring of pluripotency of hESCs without sacrificing cells. When pluripotent, these cells express EmGFP. EmGFP expression is lost upon differentiation.
2. BG01V/hOG Cells can be used as a reporter of the culture’s response to external stimuli including media composition, stress, and whether or not culture conditions are adequate to maintain hESCs in an undifferentiated state.
3. BG01V/hOG Cells can be used to determine the efficiency of your differentiation assays.
Intended use:
Research use only. Caution: Not intended for human or animal diagnostic or therapeutic uses.
Contents and Storage:
The vial of BG01V/hOG Cells should be stored in liquid nitrogen.
References:
1. Mitalipova, M. et al. (2003) Stem Cells 21: 521-526
2. Pan, G. J. et al. (2002) Cell Res 12: 321-329
3. Plaia, T. W. et al. (2006) Stem Cells 24: 531-546
Useful links:
User Manual for BG01V/hOG Cells
Embryonic Stem Cell (ESC) Product Overview Sheet
Rat Glial Precursor Cells (Gibco™)
Ready-to use system: Upon proliferation in StemPro® NSC-SFM, >80% of the cells retain their undifferentiated Phenotype, good post-thaw cell viability (≥ 50%), and supports differentiation into Oligodendrocytes and Astrocytes. Gibco® Rat Glial Precursor Cells (GPC) offer researchers ready access to high quality cells and eliminate the time and effort to isolate these highly sensitive cells in house. Glial Precursor Cells are isolated from the cortex of newborn Sprague-Dawley rats. The cells are isolated under sterile conditions, expanded in complete StemPro® NSC SFM, and cryopreserved at passage 2 (P2). Each vial contains 1 × 106 cells/ml viable cells (post-thaw) that can be expanded once to give >2 fold increase of cells. Upon proliferation in StemPro® NSC SFM, >80% of cells retain their undifferentiated Phenotype and can be differentiated into Oligodendrocytes and Astrocytes. Glial Precursor Cells are the restricted progenitors, and the majority of their down stream progeny are Oligodendrocytes and Astrocytes. Oligodendrocytes produce myelin to protect neurons and secure proper signal transduction. Demyelination is involved with critical diseases, such as multiple sclerosis, Addison-Schilder Disease (ALD) etc.
Human Episomal iPSC Line (Gibco™)
The Gibco® Human Episomal iPSC Line was derived from CD34+ cord blood using a three-plasmid, seven-factor (SOKMNLT; SOX2, OCT4 (POU5F1), KLF4, MYC, NANOG, LIN28, and SV40L T antigen) EBNA-based episomal system. This iPSC line is considered to be zero foot-print as there was no integration into the genome from the reprogramming event.It has been shown to be free of all reprogramming genes and will provide researchers with a control line to compare and study a multitude of aspects of stem cell research.
The Gibco® Human Episomal iPSC Line has a normal karyotype and endogenous expression of pluripotent markers like Oct4, Sox2, and Nanog (as shown by RT-PCR) and Oct4, SSEA4, TRA-1-60 and TRA-1-81 (as shown by ICC). Whole genome expression and epigenetic profiling analyses demonstrated that this episomal hiPSC line is molecularly indistinguishable from human embryonic stem cell lines (Burridge et al., 2011). In directed differentiation and teratoma analyses, these hiPSCs retained their differentiation potential for the ectodermal, endodermal, and mesodermal lineages (Burridge et al., 2011). In addition, vascular, hematopoietic, neural, and cardiac lineages were derived with robust efficiencies (Burridge et al., 2011).
Reference
Burridge, P.W., Thompson, S., Millrod, M.A., et al. 2011. A universal system for highly efficient cardiac differentiation of human induced pluripotent stem cells that eliminates interline variability. PLoS One, April 2011, Volume 6, Issue 4, e18293.
The Gibco® Human Episomal iPSC Line has a normal karyotype and endogenous expression of pluripotent markers like Oct4, Sox2, and Nanog (as shown by RT-PCR) and Oct4, SSEA4, TRA-1-60 and TRA-1-81 (as shown by ICC). Whole genome expression and epigenetic profiling analyses demonstrated that this episomal hiPSC line is molecularly indistinguishable from human embryonic stem cell lines (Burridge et al., 2011). In directed differentiation and teratoma analyses, these hiPSCs retained their differentiation potential for the ectodermal, endodermal, and mesodermal lineages (Burridge et al., 2011). In addition, vascular, hematopoietic, neural, and cardiac lineages were derived with robust efficiencies (Burridge et al., 2011).
Reference
Burridge, P.W., Thompson, S., Millrod, M.A., et al. 2011. A universal system for highly efficient cardiac differentiation of human induced pluripotent stem cells that eliminates interline variability. PLoS One, April 2011, Volume 6, Issue 4, e18293.
Human Neural Stem Cells (H9-Derived) (Gibco™)
With GIBCO® Human Neural Stem Cells (H9-Derived), we allow neuroscience researchers who do not have access to human embryonic stem cells or human tissues the use of the hNSC by removing the complicated isolation or derivation process. The cells are derived from the NIH approved H9 (WA09) human embryonic stem cells (hESCs). Each vial of GIBCO® hNSCs contains>1 million viable cells that can be propagated as an adherent culture in complete StemPro® NSC SFM. GIBCO® hNSCs retain their normal female human karyotype and their potential to differentiate into neurons and glial cells after multiple passages. GIBCO® Human Neural Stem Cells (H9-Derived) Kit comes with 1 vial of cells, KnockOut™ DMEM⁄F-12 Basal Medium, StemPro® Neural Supplement, FGF Basic and EGF Recombinant proteins for optimal growth and expansion as adherent cells on matrix or suspended neurospheres. Unlike tissue derived cells, hESC derived NSCs offer more consistent performance and minimize supply concerns.
- Strong performance in adherent culture in a matrix and suspended neurosphere cultures.
- Upon proliferation in StemPro® NSC SFM, >80% of the cells retain their undifferentiated Phenotype. Internal experiments have shown >90% of the cells retain their undifferentiated state.
- Supports differentiation into Neurons, Astrocytes and Oligodendrocytes.
- Sold as stand-alone cells and as a kit. Kit comes with cells and required reagents.
- Minimal variability across lots and ready-to use system.
Neural stem cells (NSCs) are self-renewing, multipotent stem cells of nervous system that can differentiate into neurons, oliogdendrocytes, and astrocytes. Multipotent NSCs can be derived from embryonic stem cells or isolated from fetal or adult central nervous system. NSCs are an invaluable resource not only for neuroscience and stem cell studies such as regulation of neurogenesis, neurotransmitter and receptor functions, and stem cell differentiation, but also for tissue engineering, cell and genetic therapy, and transplantation experiments to treat neurodegenerative diseases and neurological disorders.
- Strong performance in adherent culture in a matrix and suspended neurosphere cultures.
- Upon proliferation in StemPro® NSC SFM, >80% of the cells retain their undifferentiated Phenotype. Internal experiments have shown >90% of the cells retain their undifferentiated state.
- Supports differentiation into Neurons, Astrocytes and Oligodendrocytes.
- Sold as stand-alone cells and as a kit. Kit comes with cells and required reagents.
- Minimal variability across lots and ready-to use system.
Neural stem cells (NSCs) are self-renewing, multipotent stem cells of nervous system that can differentiate into neurons, oliogdendrocytes, and astrocytes. Multipotent NSCs can be derived from embryonic stem cells or isolated from fetal or adult central nervous system. NSCs are an invaluable resource not only for neuroscience and stem cell studies such as regulation of neurogenesis, neurotransmitter and receptor functions, and stem cell differentiation, but also for tissue engineering, cell and genetic therapy, and transplantation experiments to treat neurodegenerative diseases and neurological disorders.
Human Neural Stem Cells (H9-Derived) Kit (Gibco™)
With GIBCO® Human Neural Stem Cells (H9-Derived), we allow neuroscience researchers who do not have access to human embryonic stem cells or human tissues the use of the hNSC by removing the complicated isolation or derivation process. The cells are derived from the NIH approved H9 (WA09) human embryonic stem cells (hESCs). Each vial of GIBCO® hNSCs contains>1 million viable cells that can be propagated as an adherent culture in complete StemPro® NSC SFM. GIBCO® hNSCs retain their normal female human karyotype and their potential to differentiate into neurons and glial cells after multiple passages. GIBCO® Human Neural Stem Cells (H9-Derived) Kit comes with 1 vial of cells, KnockOut™ DMEM⁄F-12 Basal Medium, StemPro® Neural Supplement, FGF Basic and EGF Recombinant proteins for optimal growth and expansion as adherent cells on matrix or suspended neurospheres. Unlike tissue derived cells, hESC derived NSCs offer more consistent performance and minimize supply concerns.
- Strong performance in adherent culture in a matrix and suspended neurosphere cultures.
- Upon proliferation in StemPro® NSC SFM, >80% of the cells retain their undifferentiated Phenotype. Internal experiments have shown >90% of the cells retain their undifferentiated state.
- Supports differentiation into Neurons, Astrocytes and Oligodendrocytes.
- Sold as stand-alone cells and as a kit. Kit comes with cells and required reagents.
- Minimal variability across lots and ready-to use system.
Neural stem cells (NSCs) are self-renewing, multipotent stem cells of nervous system that can differentiate into neurons, oliogdendrocytes, and astrocytes. Multipotent NSCs can be derived from embryonic stem cells or isolated from fetal or adult central nervous system. NSCs are an invaluable resource not only for neuroscience and stem cell studies such as regulation of neurogenesis, neurotransmitter and receptor functions, and stem cell differentiation, but also for tissue engineering, cell and genetic therapy, and transplantation experiments to treat neurodegenerative diseases and neurological disorders.
- Strong performance in adherent culture in a matrix and suspended neurosphere cultures.
- Upon proliferation in StemPro® NSC SFM, >80% of the cells retain their undifferentiated Phenotype. Internal experiments have shown >90% of the cells retain their undifferentiated state.
- Supports differentiation into Neurons, Astrocytes and Oligodendrocytes.
- Sold as stand-alone cells and as a kit. Kit comes with cells and required reagents.
- Minimal variability across lots and ready-to use system.
Neural stem cells (NSCs) are self-renewing, multipotent stem cells of nervous system that can differentiate into neurons, oliogdendrocytes, and astrocytes. Multipotent NSCs can be derived from embryonic stem cells or isolated from fetal or adult central nervous system. NSCs are an invaluable resource not only for neuroscience and stem cell studies such as regulation of neurogenesis, neurotransmitter and receptor functions, and stem cell differentiation, but also for tissue engineering, cell and genetic therapy, and transplantation experiments to treat neurodegenerative diseases and neurological disorders.
StemPro™ BM Mesenchymal Stem Cells (Gibco™)
Les cellules souches mésenchymateuses StemPro® BM sont des cellules souches mésenchymateuses de moelle osseuse humaine cryoconservées (CSM). Ces cellules facilement disponibles sont fabriquées conformément aux bonnes pratiques de fabrication dans le respect des normes de fabrication sur un site autorisé en Californie et disponibles pour utilisation dans la recherche uniquement. Elles sont isolées et développées en culture jusqu’au passage 4 selon un procédé de fabrication exclusif, faible en oxygène (tolérant à l’ischémie (ti)), d’échelle clinique, utilisant des milieux de culture supplémentés au sérum bovin de croissance et cryoconservées dans un milieu de cryoconservation exempt de produits d’origine animale. Les cellules sont fournies à raison de 1x106 et 5x106 cellules viables par flacon. Un certificat d’analyse est disponible pour chaque lot. Il renseigne sur les spécifications de contrôle de la qualité, les résultats de test et les informations sur le donneur.
Les cellules souches mésenchymateuses StemPro® BM vous permettent :
• de minimiser la variabilité des dosages avec les CSMti fabriquées conformément aux normes de fabrication GMP ;
• d’améliorer les études de fonctionnalité avec des CSM immatures, haute puissance ;
• d’observer l’absence de tumorigénicité ou toxicité dans les études sur animaux conformes aux bonnes pratiques de laboratoire (BPL) ;
• de prendre en charge des expériences exigeant un lot important de CSM ;
• de minimiser le risque d’échec de votre essai biologique lors de l’utilisation de cellules, réactifs et protocoles testés.
CSM immatures, haute puissance, cultivées avec peu d’oxygène
Les cellules souches mésenchymateuses StemPro® BM sont dérivées d’aspirats de moelle osseuse humaine provenant de donneurs qualifiés et traçables. Les cellules sont isolées, développées et cultivées conformément aux normes de fabrication BPF dans une installation autorisée en Californie à l’aide d’un procédé de fabrication exclusif d’échelle clinique à teneur réduite en oxygène. La fabrication de cellules dans des environnements à teneur en oxygène réduite a pour résultat des rendements cellulaires plus élevés de cellules souches immatures très puissantes par rapport aux cellules développées dans des conditions de culture avec une teneur en oxygène normale.
Profils non toxiques éprouvés
Les cellules se sont avérées non toxiques et non tumorigéniques dans les études sur animaux pratiquées selon les bonnes pratiques de laboratoire et exécutées conformément aux directives de la FDA.
Un lot de grande contenance peut être mis à la disposition de ceux qui ont besoin de standardiser les essais biologiques. Les protocoles utilisant des réactifs testés seront fournis pour aider à minimiser le risque d’échec de vos essais biologiques lorsque vous utilisez des réactifs et des cellules qui n’ont pas été évalués pour fonctionner ensemble. Consultez nos milieux de culture et réactifs testés.
Viabilité élevée après la cryoconservation
Les cellules souches mésenchymateuses StemPro® sont fournies dans un milieu de cryoconservation CryoStor® à raison de 1x106 et 5x106 cellules viables par flacon. Ce milieu est exempt de produits d’origine animale et a prouvé qu’il pouvait assurer une viabilité cellulaire élevée après décongélation. Des procédés contrôlés sont utilisés pour la cryoconservation, la manipulation et le transport afin de minimiser le risque d’effets néfastes pour la qualité des cellules.
Fabriqué aux États-Unis par Stemedica pour Thermo Fisher Scientific.
Les cellules souches mésenchymateuses StemPro® BM vous permettent :
• de minimiser la variabilité des dosages avec les CSMti fabriquées conformément aux normes de fabrication GMP ;
• d’améliorer les études de fonctionnalité avec des CSM immatures, haute puissance ;
• d’observer l’absence de tumorigénicité ou toxicité dans les études sur animaux conformes aux bonnes pratiques de laboratoire (BPL) ;
• de prendre en charge des expériences exigeant un lot important de CSM ;
• de minimiser le risque d’échec de votre essai biologique lors de l’utilisation de cellules, réactifs et protocoles testés.
CSM immatures, haute puissance, cultivées avec peu d’oxygène
Les cellules souches mésenchymateuses StemPro® BM sont dérivées d’aspirats de moelle osseuse humaine provenant de donneurs qualifiés et traçables. Les cellules sont isolées, développées et cultivées conformément aux normes de fabrication BPF dans une installation autorisée en Californie à l’aide d’un procédé de fabrication exclusif d’échelle clinique à teneur réduite en oxygène. La fabrication de cellules dans des environnements à teneur en oxygène réduite a pour résultat des rendements cellulaires plus élevés de cellules souches immatures très puissantes par rapport aux cellules développées dans des conditions de culture avec une teneur en oxygène normale.
Profils non toxiques éprouvés
Les cellules se sont avérées non toxiques et non tumorigéniques dans les études sur animaux pratiquées selon les bonnes pratiques de laboratoire et exécutées conformément aux directives de la FDA.
Un lot de grande contenance peut être mis à la disposition de ceux qui ont besoin de standardiser les essais biologiques. Les protocoles utilisant des réactifs testés seront fournis pour aider à minimiser le risque d’échec de vos essais biologiques lorsque vous utilisez des réactifs et des cellules qui n’ont pas été évalués pour fonctionner ensemble. Consultez nos milieux de culture et réactifs testés.
Viabilité élevée après la cryoconservation
Les cellules souches mésenchymateuses StemPro® sont fournies dans un milieu de cryoconservation CryoStor® à raison de 1x106 et 5x106 cellules viables par flacon. Ce milieu est exempt de produits d’origine animale et a prouvé qu’il pouvait assurer une viabilité cellulaire élevée après décongélation. Des procédés contrôlés sont utilisés pour la cryoconservation, la manipulation et le transport afin de minimiser le risque d’effets néfastes pour la qualité des cellules.
Fabriqué aux États-Unis par Stemedica pour Thermo Fisher Scientific.
StemPro™ Neural Stem Cells (Gibco™)
StemPro® Neural Stem Cells are cryopreserved human fetal brain-derived neural stem cells (NSCs). These cells are manufactured under Good Manufacturing Practice (GMP) manufacturing standards in a California-licensed facility and are available for Research Use Only. They are isolated and expanded in culture to passage 4 under a low oxygen (ischemic tolerant), proprietary manufacturing process using FBS-supplemented media and then cryopreserved in an animal origin-free cryopreservation medium. The cells are supplied at a concentration of 5 x 106 viable cells/vial. A Certificate of Analysis is available for each lot that documents the quality control specifications, test results, and donor information. These cells have no observed tumorigenicity/toxicity in GLP-compliant animal studies.
StemPro® Neural Stem Cells help you to:
• Minimize assay variability with itNSCs manufactured using GMP manufacturing standards
• Improve functionality studies with immature, high potency NSCs
• Complete experiments requiring one large lot of NSCs
• Minimize your chance of assay failure by using tested cells, reagents, and protocols
Immature, High Potency, Low Oxygen-Cultured NSCs
StemPro® Neural Stem Cells are derived from human fetal brain from qualified, traceable donors. The cells are isolated, cultured, and expanded under Good Manufacturing Practice (GMP) manufacturing standards in a California-licensed facility using a proprietary Reduced Oxygen Tension manufacturing process. Manufacture of cells in a reduced oxygen tension environment results in higher yields of highly potent immature stem cells compared to cells expanded in normal oxygen culture conditions.
Safety Tested
StemPro® Neural Stem Cells have been demonstrated to be non-toxic and non-tumorigenic in GLP animal studies performed according to FDA guidelines.
Large lots can be made available for those requiring assay standardization. Protocols using tested reagents are provided to help minimize your chance of assay failure using reagents and cells that have not been evaluated to work together. View our tested media and reagents.
High Post-Cryopreservation Viability
These StemPro® Neural Stem Cells are supplied in CryoStor® cryopreservation medium at a concentration of 5x106 viable cells/vial. CryoStor® medium is animal origin-free and shown to support high cell viabilities post-thawing. Controlled processes are used for cryopreservation, handling, and shipping to help minimize risk of adverse effects to the quality of the cells.
Made in USA by Stemedica for Thermo Fisher Scientific.
StemPro® Neural Stem Cells help you to:
• Minimize assay variability with itNSCs manufactured using GMP manufacturing standards
• Improve functionality studies with immature, high potency NSCs
• Complete experiments requiring one large lot of NSCs
• Minimize your chance of assay failure by using tested cells, reagents, and protocols
Immature, High Potency, Low Oxygen-Cultured NSCs
StemPro® Neural Stem Cells are derived from human fetal brain from qualified, traceable donors. The cells are isolated, cultured, and expanded under Good Manufacturing Practice (GMP) manufacturing standards in a California-licensed facility using a proprietary Reduced Oxygen Tension manufacturing process. Manufacture of cells in a reduced oxygen tension environment results in higher yields of highly potent immature stem cells compared to cells expanded in normal oxygen culture conditions.
Safety Tested
StemPro® Neural Stem Cells have been demonstrated to be non-toxic and non-tumorigenic in GLP animal studies performed according to FDA guidelines.
Large lots can be made available for those requiring assay standardization. Protocols using tested reagents are provided to help minimize your chance of assay failure using reagents and cells that have not been evaluated to work together. View our tested media and reagents.
High Post-Cryopreservation Viability
These StemPro® Neural Stem Cells are supplied in CryoStor® cryopreservation medium at a concentration of 5x106 viable cells/vial. CryoStor® medium is animal origin-free and shown to support high cell viabilities post-thawing. Controlled processes are used for cryopreservation, handling, and shipping to help minimize risk of adverse effects to the quality of the cells.
Made in USA by Stemedica for Thermo Fisher Scientific.
StemPro™ Human Adipose-Derived Stem Cell Kit (Gibco™)
Human adipose-derived stem cells (ADSCs) are isolated from human lipoaspirate tissue and cryopreserved from primary cultures. ADSCs have phenotypic and functional characteristics very similar to those of bone marrow-derived mesenchymal stem cells. Before cryopreservation, the ADSCs are expanded for one passage in MesenPRO RS™ Medium, a reduced-serum (2%) medium that improves MSC expansion compared to conventional serum supplemented media. The StemPro® Human Adipose-Derived Stem Cell Kit contains cryopreserved normal human ADSCs and MesenPRO RS™ Medium - together, these components ensure optimal ADSC growth and expansion. Each lot of ADSCs originates from a single donor of human lipoaspirate tissue. Each vial of ADSCs contains cells that can differentiate into multiple mature cell phenotypes in vitro, including adipocytes, osteoblasts, and chondrocytes. ADSCs can be expanded to 4-5 passages before they lose their ability to grow or differentiate into all potential phenotypes. The ADSCs express the following flow cytometry cell-surface protein profile: positive CD29, CD44, CD73, CD90, CD105, CD166; negative CD14, CD31, CD45, Lin1.
Applications: Formulated for the growth and expansion of mesenchymal stem cells.
Note: MesenPRO RS™ Medium (Cat. no. 12746-012), composed of MesenPRO RS™ Growth Supplement and MesenPRO RS™ Basal Medium, is available for purchase separately.
Applications: Formulated for the growth and expansion of mesenchymal stem cells.
Note: MesenPRO RS™ Medium (Cat. no. 12746-012), composed of MesenPRO RS™ Growth Supplement and MesenPRO RS™ Basal Medium, is available for purchase separately.
Rat Fetal Neural Stem Cell Kit (Gibco™)
GIBCO® Rat Fetal Neural Stem Cells provide superior performance in both adherent cell culture in a matrix and suspended neurosphere cultures. The cells are isolated from the cortex of Sprague-Dawley® rats at day E14 of gestation. Each vial contains 2 × 106 viable cells. For optimal growth performance, we recommend culturing and expanding the cells in StemPro® NSC serum-free media.
Features of the Gibco® Rat Fetal Neural Stem Cell Kit include:
• Superior performance in adherent and spheroid cultures
• >75% of the cells retain their undifferentiated phenotype
• Supports differentiation into neurons, astrocytes, and oligodendrocytes
• Kit comes with cells and associated culture reagents
About neural stem cells
Neural Stem Cells (NSC) are self-renewing multipotent stem cells of nervous system that can differentiate into neurons, oliogdendrocytes, and astrocytes. Multipotent neural stem cells can be isolated from fetal or adult central nervous system tissue, or derived from embryonic stem cells. In development, neurogenesis takes place in the third to fourth week of gestation and continues in specific regions of the central nervous system. Because of their capacity to generate neurons and glial cells, neural stem cells are a valuable resource, not only for neuroscience, but also for the potential treatment of neurodegenerative disease and neurological disorders. Adult-derived NSCs tend to be regionally restricted and have limited plasticity compared to fetal-derived NSC.
Features of the Gibco® Rat Fetal Neural Stem Cell Kit include:
• Superior performance in adherent and spheroid cultures
• >75% of the cells retain their undifferentiated phenotype
• Supports differentiation into neurons, astrocytes, and oligodendrocytes
• Kit comes with cells and associated culture reagents
About neural stem cells
Neural Stem Cells (NSC) are self-renewing multipotent stem cells of nervous system that can differentiate into neurons, oliogdendrocytes, and astrocytes. Multipotent neural stem cells can be isolated from fetal or adult central nervous system tissue, or derived from embryonic stem cells. In development, neurogenesis takes place in the third to fourth week of gestation and continues in specific regions of the central nervous system. Because of their capacity to generate neurons and glial cells, neural stem cells are a valuable resource, not only for neuroscience, but also for the potential treatment of neurodegenerative disease and neurological disorders. Adult-derived NSCs tend to be regionally restricted and have limited plasticity compared to fetal-derived NSC.
StemPro™ CD34+ Cell Kit
StemPro® CD34+ cells are pooled Human Hematopoietic Progenitor cells (HPCs) derived from the umbilical cord blood of mixed donors. Along with 0.5×106 pooled donor cord blood-derived CD34+ cells, this kit includes StemPro®-34 SFM basal liquid medium and frozen StemPro®-34 Nutrient Supplement to facilitate the immediate culturing of the StemPro® CD34+ cells upon arrival or when recovering from cryopreservation storage.
With the StemPro® CD34+ Cell Kit You Get:
• ≥90% purity of the desired CD34+ cell population
• Each vial of cells contains 0.5×106 CD34+ cells with full traceability
• StemPro®-34 SFM manufactured following cGMP and ISO13485
• High reprogramming efficiency to iPSCs
• Custom sizes available upon request
Included in this kit are:
• One vial containing one ml of 0.5×106 cells/ml pooled donor cord blood-derived CD34+ cells
• 500 ml StemPro®-34 SFM basal liquid medium
• 13 ml frozen StemPro®-34 Nutrient Supplement
Hematopoietic Progenitor Cells
Hematopoietic progenitor cells (HPCs) or hematopoietic stem cells (HSCs) can be found in the blood, bone marrow, umbilical cord blood, or derived from Embryonic Stem Cells (ESCs) or induced pluripotent stem cells (iPSCs). They are multipotent stem cells capable of forming every cell lineage in the blood and immune system. Hematopoietic progenitor cells (HPCs) are non-adherent, with a small, round morphology. A fundamental property of these cells is their capacity to self-renew, thus providing, in principle, a limitless source of daughter cells with the same functional properties. Several cell surface markers used for identifying human HPCs/HSCs include CD34+, CD59+, Thy1/CD90+, CD38 lo/-, C-kit/CD117+, Lin-.
Common Applications
Hematopoietic stem cells (HSCs) are most commonly used in transplantation therapies to treat illnesses such as leukemia, lymphoma, and sickle cell diseases by reconstituting a patient's hematopoietic cell system.
High Purity Stem Cells for Higher Reprogramming Efficiency
HPCs/HSCs can be reprogrammed to induced pluripotent stem cells (iPSCs) for use as disease models in stem cell research. StemPro® CD34+ cells have ≥90% CD34+ cells, as determined by Flow Cytometry, affording higher reprogramming efficiency than less pure populations. Protocols using CytoTune®-iPS Sendai Reprogramming Kit are available on our website and included in the product insert.
Isolation of CD34+ Cells from Cord Blood
StemPro® CD34+ primary cord blood cells from multiple human donors originating and collected in the United States are pooled and then enriched for the CD34+ cell population using immunomagnetic CD34 MicroBeads. Pooling the cord blood from multiple donors reduces the lot-to-lot variability of the product. The cells are derived from tissue obtained from accredited institutions through an IRB (Institutional Review Board) approved donor program and are negative for HIV and Hepatitis B.
Quality control
QC for the StemPro® CD34+ cells includes testing for the following: mycoplasma, sterility, endotoxin, and FACS profile.
QC for StemPro-34 SFM includes testing for the following: Sterility, pH, endotoxin, osmolality, Hepatitis B Surface Antigen, HIV 1&2 antibody, HCV antibody, and CD34+ cell performance assay.
Serum Free Medium Developed for CD34+ Cell Expansion
StemPro®-34 SFM is comprised of a basal liquid medium and frozen StemPro®-34 Nutrient Supplement. This serum-free media system was developed to support the growth of human CD34+ hematopoietic progenitor cells. Sera can vary from lot to lot in its ability to support cell-specific growth in culture. Serum-free culture systems eliminate this major variable in the study of hematopoiesis ex vivo. StemPro®-34 SFM can be purchased separately.
See a list of selected publications citing the Sendai virus for iPSC generation here.
For Research Use Only. Not for use in diagnostic procedures.
With the StemPro® CD34+ Cell Kit You Get:
• ≥90% purity of the desired CD34+ cell population
• Each vial of cells contains 0.5×106 CD34+ cells with full traceability
• StemPro®-34 SFM manufactured following cGMP and ISO13485
• High reprogramming efficiency to iPSCs
• Custom sizes available upon request
Included in this kit are:
• One vial containing one ml of 0.5×106 cells/ml pooled donor cord blood-derived CD34+ cells
• 500 ml StemPro®-34 SFM basal liquid medium
• 13 ml frozen StemPro®-34 Nutrient Supplement
Hematopoietic Progenitor Cells
Hematopoietic progenitor cells (HPCs) or hematopoietic stem cells (HSCs) can be found in the blood, bone marrow, umbilical cord blood, or derived from Embryonic Stem Cells (ESCs) or induced pluripotent stem cells (iPSCs). They are multipotent stem cells capable of forming every cell lineage in the blood and immune system. Hematopoietic progenitor cells (HPCs) are non-adherent, with a small, round morphology. A fundamental property of these cells is their capacity to self-renew, thus providing, in principle, a limitless source of daughter cells with the same functional properties. Several cell surface markers used for identifying human HPCs/HSCs include CD34+, CD59+, Thy1/CD90+, CD38 lo/-, C-kit/CD117+, Lin-.
Common Applications
Hematopoietic stem cells (HSCs) are most commonly used in transplantation therapies to treat illnesses such as leukemia, lymphoma, and sickle cell diseases by reconstituting a patient's hematopoietic cell system.
High Purity Stem Cells for Higher Reprogramming Efficiency
HPCs/HSCs can be reprogrammed to induced pluripotent stem cells (iPSCs) for use as disease models in stem cell research. StemPro® CD34+ cells have ≥90% CD34+ cells, as determined by Flow Cytometry, affording higher reprogramming efficiency than less pure populations. Protocols using CytoTune®-iPS Sendai Reprogramming Kit are available on our website and included in the product insert.
Isolation of CD34+ Cells from Cord Blood
StemPro® CD34+ primary cord blood cells from multiple human donors originating and collected in the United States are pooled and then enriched for the CD34+ cell population using immunomagnetic CD34 MicroBeads. Pooling the cord blood from multiple donors reduces the lot-to-lot variability of the product. The cells are derived from tissue obtained from accredited institutions through an IRB (Institutional Review Board) approved donor program and are negative for HIV and Hepatitis B.
Quality control
QC for the StemPro® CD34+ cells includes testing for the following: mycoplasma, sterility, endotoxin, and FACS profile.
QC for StemPro-34 SFM includes testing for the following: Sterility, pH, endotoxin, osmolality, Hepatitis B Surface Antigen, HIV 1&2 antibody, HCV antibody, and CD34+ cell performance assay.
Serum Free Medium Developed for CD34+ Cell Expansion
StemPro®-34 SFM is comprised of a basal liquid medium and frozen StemPro®-34 Nutrient Supplement. This serum-free media system was developed to support the growth of human CD34+ hematopoietic progenitor cells. Sera can vary from lot to lot in its ability to support cell-specific growth in culture. Serum-free culture systems eliminate this major variable in the study of hematopoiesis ex vivo. StemPro®-34 SFM can be purchased separately.
See a list of selected publications citing the Sendai virus for iPSC generation here.
For Research Use Only. Not for use in diagnostic procedures.
Rat Fetal Neural Stem Cells (Gibco™)
Superior performance in adherent and spheroid cultures. >75% of the cells retain their undifferentiated Phenotype. Supports differentiation into Neurons, Astrocytes and Oligodendrocytes.
Gibco® Rat Fetal Neural Stem Cells enable superior performance in both adherent cell culture in a matrix and suspended neurosphere cultures. The cells are isolated from the cortex of Sprague-Dawley at day E14 of gestation. Each vial contains 2 × 10^6 viable cells⁄ml. For optimal growth performance, we recommend culturing and expanding the cells in StemPro® NSC SFM (sku #: A1050901) media. Upon proliferation in StemPro® NSC SFM, >75% of the cells retain their undifferentiated Phenotype and can be differentiated into Neurons, Astrocytes and Oligodendrocytes.
For a limited time, we offer introductory pricing for you to try the cells. Don't miss this opportunity!
Neural Stem Cells (NSC) are self renewing multipotent stem cells of nervous system that can differentiate into neurons, oliogdendrocyte and astrocyte. Multipotent neural stem cells can be isolated from fetal or adult central nervous system or derived from embryonic stem cells. In development, neurogenesis takes place third to fourth week of gestation and continued in specific region of our central nervous system. Because of their property to generate neurons and glial, NSC is a valuable source not only for neuroscience but also for clinical use to treat neurodegenerative disease or neurological disorder. Adult derived NSC tends to be regionally restricted and have limited plasticity compared to fetal derived NSC.
Gibco® Rat Fetal Neural Stem Cells enable superior performance in both adherent cell culture in a matrix and suspended neurosphere cultures. The cells are isolated from the cortex of Sprague-Dawley at day E14 of gestation. Each vial contains 2 × 10^6 viable cells⁄ml. For optimal growth performance, we recommend culturing and expanding the cells in StemPro® NSC SFM (sku #: A1050901) media. Upon proliferation in StemPro® NSC SFM, >75% of the cells retain their undifferentiated Phenotype and can be differentiated into Neurons, Astrocytes and Oligodendrocytes.
For a limited time, we offer introductory pricing for you to try the cells. Don't miss this opportunity!
Neural Stem Cells (NSC) are self renewing multipotent stem cells of nervous system that can differentiate into neurons, oliogdendrocyte and astrocyte. Multipotent neural stem cells can be isolated from fetal or adult central nervous system or derived from embryonic stem cells. In development, neurogenesis takes place third to fourth week of gestation and continued in specific region of our central nervous system. Because of their property to generate neurons and glial, NSC is a valuable source not only for neuroscience but also for clinical use to treat neurodegenerative disease or neurological disorder. Adult derived NSC tends to be regionally restricted and have limited plasticity compared to fetal derived NSC.
