Shop All DNA⁄RNA Purification Kits & Reagents
Ion AmpliSeq™ Dynabeads™ Cleanup Module (Ion Torrent™)
The Ion AmpliSeq™ Dynabeads® Cleanup Module is a magnetic bead-based purification kit used in the construction of RNA libraries for sequencing on the Ion Personal Genome Machine® (PGM™) or Ion Proton™ sequencer. This module is included in the Ion AmpliSeq™ RNA Library Kit, but may be purchased separately if additional modules are needed.
For RNA library construction using the Ion AmpliSeq™ RNA Library Kit, the module is used for cleanup after the cDNA synthesis and PCR amplification steps. The module employs Dynabeads® magnetic beads for DNA adsorption and separation, rather than traditional filter columns, for quick and easy processing and simplified automation.
This module is not for use alone or with any kits other than the Ion AmpliSeq™ RNA Library Kit.
For RNA library construction using the Ion AmpliSeq™ RNA Library Kit, the module is used for cleanup after the cDNA synthesis and PCR amplification steps. The module employs Dynabeads® magnetic beads for DNA adsorption and separation, rather than traditional filter columns, for quick and easy processing and simplified automation.
This module is not for use alone or with any kits other than the Ion AmpliSeq™ RNA Library Kit.
ChargeSwitch™ gDNA Plant Kit (Invitrogen™)
The ChargeSwitch® gDNA Plant Kit uses magnetic bead-based technology to allow rapid and efficient isolation of genomic DNA from fungal and plant samples, including leaves, tissues and seeds, in less than 15 minutes after lysate preparation.
• Optimized for higher-purity DNA extraction
• Compatible with high-throughput protocols
• Ideal for a variety of downstream applications
Rapid, Three Step, pH Based Purification Protocol
The ChargeSwitch® gDNA Plant Kit allows purification of genomic DNA from between 50-100 mg of fungi and plant samples, including leaves, tissues and seeds (Figure 1), to yield up to 7 µg of gDNA typically in less than 15 minutes following sample preparation and lysis. The ChargeSwitch® gDNA Plant Kit uses magnetic beads with a unique, ionizable functional group whose affinity for nucleic acids is pH-dependent to facilitate nucleic acid purification. Genomic DNA is isolated without the use of centrifugation or organic solvents, and is of higher purity than silica-prepared DNA, which is typically contaminated with guanidine salts.
Compatible with Single Tube and High-Throughput Protocols
The ChargeSwitch® gDNA Plant Kit is designed for automated processing of both small numbers of samples individually, as well as large numbers of samples using a liquid handling robot or a multi-channel pipette.
For processing up to 24 individual samples at a time the MagnaRack™ two-piece magnetic separation tube rack is required. This rack consists of a magnetic base station and a removable tube rack, which can hold up to 24 microcentrifuge tubes and can be used with most ChargeSwitch® products.
For high-throughput applications utilizing 96 well blocks, the 96-Well Magnetic Separator is required. This rack can hold and separate up to 96 magnetic bead samples in a deep well plate when placed on top of the magnetic base station.
Improved Performance in Demanding Downstream Applications
The ChargeSwitch® gDNA Plant Kit ensures reliable performance of the resulting gDNA and is routinely used for the most sensitive applications, including genetically modified organism (GMO) screening. Other downstream applications include PCR, restriction enzyme digestion, and Southern blotting.
For Research Use Only. Not intended for diagnostic procedures.
• Optimized for higher-purity DNA extraction
• Compatible with high-throughput protocols
• Ideal for a variety of downstream applications
Rapid, Three Step, pH Based Purification Protocol
The ChargeSwitch® gDNA Plant Kit allows purification of genomic DNA from between 50-100 mg of fungi and plant samples, including leaves, tissues and seeds (Figure 1), to yield up to 7 µg of gDNA typically in less than 15 minutes following sample preparation and lysis. The ChargeSwitch® gDNA Plant Kit uses magnetic beads with a unique, ionizable functional group whose affinity for nucleic acids is pH-dependent to facilitate nucleic acid purification. Genomic DNA is isolated without the use of centrifugation or organic solvents, and is of higher purity than silica-prepared DNA, which is typically contaminated with guanidine salts.
Compatible with Single Tube and High-Throughput Protocols
The ChargeSwitch® gDNA Plant Kit is designed for automated processing of both small numbers of samples individually, as well as large numbers of samples using a liquid handling robot or a multi-channel pipette.
For processing up to 24 individual samples at a time the MagnaRack™ two-piece magnetic separation tube rack is required. This rack consists of a magnetic base station and a removable tube rack, which can hold up to 24 microcentrifuge tubes and can be used with most ChargeSwitch® products.
For high-throughput applications utilizing 96 well blocks, the 96-Well Magnetic Separator is required. This rack can hold and separate up to 96 magnetic bead samples in a deep well plate when placed on top of the magnetic base station.
Improved Performance in Demanding Downstream Applications
The ChargeSwitch® gDNA Plant Kit ensures reliable performance of the resulting gDNA and is routinely used for the most sensitive applications, including genetically modified organism (GMO) screening. Other downstream applications include PCR, restriction enzyme digestion, and Southern blotting.
For Research Use Only. Not intended for diagnostic procedures.
PureLink™ Pro 96 total RNA Purification Kit (Invitrogen™)
The PureLink® Pro 96 total RNA Purification Kit provides high-throughput isolation of high-quality total RNA from the widest range of sample types and sizes. Isolate high yields of total RNA with low genomic DNA contamination from bacteria, yeast, plant, or mammalian samples (Figures 1 and 2).
The one-hour procedure requires no handling or disposal of organic waste. RNA binds to a silica membrane and impurities are removed by washing through vacuum filtration or centrifugation. High-purity total RNA is eluted into RNase-free water; ideal for use in a variety of applications, including RT-PCR, qPCR, northern blotting, and nuclease protection assays.
Improved plate design
The PureLink® Pro 96 total RNA Purification Kit provides optimized semi-skirted 96-well plates(Figure 3) for improved performance and results. In addition, the plate nozzles with annular collar prevent cross-contamination of samples and improve drying of the silica membrane to prevent ethanol carryover. In addition, sample processing can be performed manually using a benchtop vacuum manifold, by centrifugation (at 2250 x g), or with an automated liquid handling platform. The semi-skirted plate design is compatible with most vacuum manifolds on robotic workstations.
The one-hour procedure requires no handling or disposal of organic waste. RNA binds to a silica membrane and impurities are removed by washing through vacuum filtration or centrifugation. High-purity total RNA is eluted into RNase-free water; ideal for use in a variety of applications, including RT-PCR, qPCR, northern blotting, and nuclease protection assays.
Improved plate design
The PureLink® Pro 96 total RNA Purification Kit provides optimized semi-skirted 96-well plates(Figure 3) for improved performance and results. In addition, the plate nozzles with annular collar prevent cross-contamination of samples and improve drying of the silica membrane to prevent ethanol carryover. In addition, sample processing can be performed manually using a benchtop vacuum manifold, by centrifugation (at 2250 x g), or with an automated liquid handling platform. The semi-skirted plate design is compatible with most vacuum manifolds on robotic workstations.
PrepFiler Express BTA™ Forensic DNA Extraction Kit (Applied Biosystems™)
This kit is for use with the AutoMate Express™ Forensic DNA Extraction System. It was designed specifically for challenging samples such as bones, teeth and adhesive based samples such as cigarette butts and tape lifts. We have designed the PrepFiler® chemistry specifically to improve the quantity and quality of DNA isolated from forensic samples, thereby increasing the potential to obtain maximum information from downstream Short Tandem Repeat (STR) analysis.
Key Product Features:
• Designed to improve the yield and overall purity of DNA isolated from both routine and challenging forensic samples.
• Provides high quality DNA, free of PCR inhibitors and suitable for downstream applications such as quantitative real-time PCR and short tandem repeat (STR) analysis
• Simple and fast separation of substrate from lysate with the unique PrepFiler® LySep™ Column
• Quick and easy run setup with ready-to-use pre-filled cartridges
• Closed system that minimizes the risk of contamination and transposition errors
• Processes 1 to 13 samples in a single run
• Rapid implementation with pre-validated, pre-programmed instrument protocols
• Integrated solution including novel reagents, consumables and automation to provide time saving, cost effective and reliable DNA extraction
• Improves downstream genotyping (STR) success rate and profile quality
Streamlined Off-Line Lysis
The PrepFiler Express™ Kits include the innovative PrepFiler LySep™ Column, which dramatically streamlines the off-line lysis portion of the extraction method. PrepFiler™ Lysis buffer or PrepFiler BTA™ Lysis buffer is added directly into the PrepFiler® LySep™ Column along with the sample for incubation. Next, the PrepFiler® LySep™ Column is quickly centrifuged which permits lysate flow through the burstable membrane while the substrate remains behind in the column. The sample tube is then simply ready to be placed on the AutoMate Express™ instrument. This eliminates the manual lysate and substrate transfer steps, saving time and minimizing cross contamination and sample transposition events.
For Research, Forensic, or Paternity Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Key Product Features:
• Designed to improve the yield and overall purity of DNA isolated from both routine and challenging forensic samples.
• Provides high quality DNA, free of PCR inhibitors and suitable for downstream applications such as quantitative real-time PCR and short tandem repeat (STR) analysis
• Simple and fast separation of substrate from lysate with the unique PrepFiler® LySep™ Column
• Quick and easy run setup with ready-to-use pre-filled cartridges
• Closed system that minimizes the risk of contamination and transposition errors
• Processes 1 to 13 samples in a single run
• Rapid implementation with pre-validated, pre-programmed instrument protocols
• Integrated solution including novel reagents, consumables and automation to provide time saving, cost effective and reliable DNA extraction
• Improves downstream genotyping (STR) success rate and profile quality
Streamlined Off-Line Lysis
The PrepFiler Express™ Kits include the innovative PrepFiler LySep™ Column, which dramatically streamlines the off-line lysis portion of the extraction method. PrepFiler™ Lysis buffer or PrepFiler BTA™ Lysis buffer is added directly into the PrepFiler® LySep™ Column along with the sample for incubation. Next, the PrepFiler® LySep™ Column is quickly centrifuged which permits lysate flow through the burstable membrane while the substrate remains behind in the column. The sample tube is then simply ready to be placed on the AutoMate Express™ instrument. This eliminates the manual lysate and substrate transfer steps, saving time and minimizing cross contamination and sample transposition events.
For Research, Forensic, or Paternity Use Only. Not intended for any animal or human therapeutic or diagnostic use.
TRIzol™ LS Reagent (Invitrogen™)
Le réactif TRIzol® LS est un réactif complet et prêt à l’emploi optimisé pour l’isolement d’ARN total de haute qualité ou l’isolement simultanée d’ARN, d’ADN et de protéines à partir d’une variété d’échantillons liquides. Cette solution monophasée de phénol et d’isothiocyanate de guanidine est conçue pour isoler des fractions séparées d’ARN, d’ADN et de protéines à partir d’échantillons liquides de tissus d’origine humaine, animale, végétale, de levures, bactérienne ou virale, généralement en moins d’une heure. Principales caractéristiques du réactif TRIzol® LS :
• Formulé pour une utilisation avec des échantillons liquides, comme les préparations de sérum et de virus
• Facilite le rétablissement aux valeurs de consigne d’ARN, d’ADN et de protéines à partir d’un échantillon liquide unique
• Offre une excellente capacité de lyse, même avec des liquides biologiques difficiles
Purifiez l’ARN de façon fiable à partir de multiples sources d’échantillons
Le réactif TRIzol® LS est conçu pour le traitement d’une vaste gamme d’échantillons liquides d’un volume maximal de 0,25 ml. Le réactif TRIzol® LS diffère du réactif TRIzol® standard en termes de concentration, ce qui permet de traiter un plus grand nombre d’échantillons. Tout comme pour le réactif TRIzol® standard, l’intégrité des préparations d’ARN obtenues est garantie par l’inhibition très efficace de l’activité RNase pendant l’homogénéisation des échantillons. La simplicité de la méthode utilisant le réactif TRIzol® LS permet le traitement simultané d’un grand nombre d’échantillons. Cette méthode peut être entièrement réalisée en 1 heure. L’ARN total isolé grâce au réactif TRIzol® LS est exempt de protéines et de contamination d’ADN.
Formulé pour traiter plusieurs isolements
Le réactif TRIzol® LS vous permet de réaliser des précipitations séquentielles d’ARN, d’ADN et de protéines à partir d’un seul échantillon. Après l’homogénéisation des échantillons à l’aide du réactif TRIzol® LS, du chloroforme est ajouté et l’homogénat peut alors se séparer en une couche aqueuse supérieure transparente (contenant l’ARN), une interface, et en une couche organique inférieure rouge (contenant ADN et protéines). L’ARN est précipité à partir de la couche aqueuse à l’aide d’isopropanol. L’ADN est précipité à partir de l’interface aqueuse /organique avec de l’éthanol. Les protéines sont précipitées à partir de la couche phénol-éthanol par précipitation d’isopropanol. L’ARN, l’ADN ou les protéines précipitées sont alors lavées pour éliminer les impuretés, puis remises en suspension pour utilisation dans des applications en aval.
Les produits purifiés conviennent parfaitement à une grande variété d’applications.
L’ARN isolé peut être utilisé dans le cadre d’une PCR quantitative en temps réel (qPCR), d’une analyse par transfert Northern, d’une hybridation ponctuelle, d’une sélection poly-(A)+, d’une traduction in vitro, d’essais de protection contre la RNase et d’un clonage moléculaire. L’ADN isolé peut être utilisé en PCR, ainsi que pour la digestion par des enzymes de restriction et les transferts Southern. Les protéines isolées peuvent être utilisées pour les transferts Western, le rétablissement aux valeurs de consigne de l’activité enzymatique et d’une partie de l’immunoprécipitation
• Formulé pour une utilisation avec des échantillons liquides, comme les préparations de sérum et de virus
• Facilite le rétablissement aux valeurs de consigne d’ARN, d’ADN et de protéines à partir d’un échantillon liquide unique
• Offre une excellente capacité de lyse, même avec des liquides biologiques difficiles
Purifiez l’ARN de façon fiable à partir de multiples sources d’échantillons
Le réactif TRIzol® LS est conçu pour le traitement d’une vaste gamme d’échantillons liquides d’un volume maximal de 0,25 ml. Le réactif TRIzol® LS diffère du réactif TRIzol® standard en termes de concentration, ce qui permet de traiter un plus grand nombre d’échantillons. Tout comme pour le réactif TRIzol® standard, l’intégrité des préparations d’ARN obtenues est garantie par l’inhibition très efficace de l’activité RNase pendant l’homogénéisation des échantillons. La simplicité de la méthode utilisant le réactif TRIzol® LS permet le traitement simultané d’un grand nombre d’échantillons. Cette méthode peut être entièrement réalisée en 1 heure. L’ARN total isolé grâce au réactif TRIzol® LS est exempt de protéines et de contamination d’ADN.
Formulé pour traiter plusieurs isolements
Le réactif TRIzol® LS vous permet de réaliser des précipitations séquentielles d’ARN, d’ADN et de protéines à partir d’un seul échantillon. Après l’homogénéisation des échantillons à l’aide du réactif TRIzol® LS, du chloroforme est ajouté et l’homogénat peut alors se séparer en une couche aqueuse supérieure transparente (contenant l’ARN), une interface, et en une couche organique inférieure rouge (contenant ADN et protéines). L’ARN est précipité à partir de la couche aqueuse à l’aide d’isopropanol. L’ADN est précipité à partir de l’interface aqueuse /organique avec de l’éthanol. Les protéines sont précipitées à partir de la couche phénol-éthanol par précipitation d’isopropanol. L’ARN, l’ADN ou les protéines précipitées sont alors lavées pour éliminer les impuretés, puis remises en suspension pour utilisation dans des applications en aval.
Les produits purifiés conviennent parfaitement à une grande variété d’applications.
L’ARN isolé peut être utilisé dans le cadre d’une PCR quantitative en temps réel (qPCR), d’une analyse par transfert Northern, d’une hybridation ponctuelle, d’une sélection poly-(A)+, d’une traduction in vitro, d’essais de protection contre la RNase et d’un clonage moléculaire. L’ADN isolé peut être utilisé en PCR, ainsi que pour la digestion par des enzymes de restriction et les transferts Southern. Les protéines isolées peuvent être utilisées pour les transferts Western, le rétablissement aux valeurs de consigne de l’activité enzymatique et d’une partie de l’immunoprécipitation
TRIzol™ Max™ Bacterial RNA Isolation Kit (Invitrogen™)
The TRIzol® Max™ Bacterial RNA Isolation Kit provides a simple and reliable method to improve the isolation of intact total RNA from gram-positive and gram-negative bacteria. The kit utilizes both the Max™ Bacterial Enhancement Reagent and TRIzol® Reagent to inactivate endogenous RNases and promote protein denaturing, improving RNA quality and integrity. Isolation of RNA takes about an hour.
Key Features of the TRIzol® Max™ Bacterial RNA Isolation Kit:
• Formulated for the isolation of RNA from both Gram-positive and Gram-negative bacteria
• Uses two technologies for maximum RNA yield and purity
• Minimizes time required for RNA isolation by eliminating enzymatic and mechanical lytic steps
Two Products in One Kit
The TRIzol® Max™ Bacterial RNA Isolation Kit combines Max™ Bacterial Enhancement Reagent with TRIzol® Reagent. A 5-minute pre-treatment of bacteria with Max™ Bacterial Enhancement Reagent, containing chelating agents, detergent and buffer, denatures bacterial proteins and effectively deactivates endogenous RNases. Subsequent bacterial lysis by TRIzol® Reagent generates a high-quality, minimally degraded RNA product.
Purified RNA is Ideal for Use with a Variety of Applications and Products
Bacterial RNA isolated using the TRIzol® Max™ Bacterial RNA Isolation Kit is suitable for downstream applications such as real-time PCR (qPCR), RT-PCR, northern blotting, nuclease protection assays, cDNA synthesis, microarray analysis, and dot blot hybridization.
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
Key Features of the TRIzol® Max™ Bacterial RNA Isolation Kit:
• Formulated for the isolation of RNA from both Gram-positive and Gram-negative bacteria
• Uses two technologies for maximum RNA yield and purity
• Minimizes time required for RNA isolation by eliminating enzymatic and mechanical lytic steps
Two Products in One Kit
The TRIzol® Max™ Bacterial RNA Isolation Kit combines Max™ Bacterial Enhancement Reagent with TRIzol® Reagent. A 5-minute pre-treatment of bacteria with Max™ Bacterial Enhancement Reagent, containing chelating agents, detergent and buffer, denatures bacterial proteins and effectively deactivates endogenous RNases. Subsequent bacterial lysis by TRIzol® Reagent generates a high-quality, minimally degraded RNA product.
Purified RNA is Ideal for Use with a Variety of Applications and Products
Bacterial RNA isolated using the TRIzol® Max™ Bacterial RNA Isolation Kit is suitable for downstream applications such as real-time PCR (qPCR), RT-PCR, northern blotting, nuclease protection assays, cDNA synthesis, microarray analysis, and dot blot hybridization.
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
MagMAX™ Plant RNA Isolation Kit (Applied Biosystems™)
The MagMAX Plant RNA Isolation Kit is designed for automated high-throughput or manual purification of total RNA from wide variety of plant species and tissue types. It utilizes paramagnetic bead technology, enabling high yields and robust performance. The high binding capacity, uniform particle size, and rapid magnetic response of MagMAX magnetic beads makes the technology ideal for high-throughput automatic nucleic acid purification, as well as for manual nucleic acid purification by low- throughput users. The resulting high quality purified RNA is free of proteins, nucleases, and other contaminants or inhibitors, and can be used in a wide range of downstream applications, such as RT-PCR, RT-qPCR, and other enzymatic reactions.
• Versatile—suitable for any plant type and part, including woody, polyphenol-rich, and lignified samples
• High yields of pure total RNA
• High quality—isolates intact total RNA, free of inhibitors and gDNA contamination. Isolated RNA is suitable for long term storage at -20°C.
• Flexible—easily adjustable for high-throughput automation platforms and manual processing
Applications
The MagMAX Plant RNA Isolation Kit is designed for fast extraction of high purity RNA suitable for all conventional molecular biology applications, including:
• RT-PCR
• RT-qPCR
• Northern blotting
• Nuclease protection assay
Benefits of MagMAX magnetic bead–based purification
The magnetic bead–based purification format of the kit allows for easy scaling of the number of samples processed either manually with a magnetic stand or with a magnetic particle processor such as the KingFisher Duo Prime or KingFisher Flex processors.
Magnetic beads offer many benefits compared to other technologies for isolating nucleic acids. Beads bind the RNA and DNA more efficiently than glass fiber filters, resulting in higher and more consistent yields. Additionally, because filters and vacuum manifolds are not used, there is no risk of undigested tissue particulates clogging these items during the extraction process. This clogging issue is of particular concern with fibrous plant tissue samples that that are not fully lysed during the disruption and homogenization steps.
Optimized bead surface chemistry
The Dynabeads MyOne Carboxylic Acid utilized in the MagMAX Plant RNA Isolation Kit has an optimized surface chemistry with high specific surface area that allows efficient kinetics and high sensitivity in nucleic acid capture. These aspects make Dynabeads magnetic technology ideal for the recovery of the RNA targets found in a variety of plant samples. Once captured, the RNA is then eluted in 100 µL and ready for downstream applications.
• Versatile—suitable for any plant type and part, including woody, polyphenol-rich, and lignified samples
• High yields of pure total RNA
• High quality—isolates intact total RNA, free of inhibitors and gDNA contamination. Isolated RNA is suitable for long term storage at -20°C.
• Flexible—easily adjustable for high-throughput automation platforms and manual processing
Applications
The MagMAX Plant RNA Isolation Kit is designed for fast extraction of high purity RNA suitable for all conventional molecular biology applications, including:
• RT-PCR
• RT-qPCR
• Northern blotting
• Nuclease protection assay
Benefits of MagMAX magnetic bead–based purification
The magnetic bead–based purification format of the kit allows for easy scaling of the number of samples processed either manually with a magnetic stand or with a magnetic particle processor such as the KingFisher Duo Prime or KingFisher Flex processors.
Magnetic beads offer many benefits compared to other technologies for isolating nucleic acids. Beads bind the RNA and DNA more efficiently than glass fiber filters, resulting in higher and more consistent yields. Additionally, because filters and vacuum manifolds are not used, there is no risk of undigested tissue particulates clogging these items during the extraction process. This clogging issue is of particular concern with fibrous plant tissue samples that that are not fully lysed during the disruption and homogenization steps.
Optimized bead surface chemistry
The Dynabeads MyOne Carboxylic Acid utilized in the MagMAX Plant RNA Isolation Kit has an optimized surface chemistry with high specific surface area that allows efficient kinetics and high sensitivity in nucleic acid capture. These aspects make Dynabeads magnetic technology ideal for the recovery of the RNA targets found in a variety of plant samples. Once captured, the RNA is then eluted in 100 µL and ready for downstream applications.
MagJET Plant Genomic DNA Kit (Thermo Scientific™)
Thermo Scientific MagJET Plant Genomic DNA Kit is designed for automated high throughput or manual purification of genomic DNA from a wide variety of plant species. The kit exploits an efficient magnetic particle technology eliminating need for phenol/chloroform extraction or alcohol precipitation.
The MagJET Plant Genomic DNA Kit procedures are optimized for the isolation of genomic DNA from 10-100 mg plant samples. DNA yields vary between different species and tissues depending on genome size, ploidy, cell number, and age of tissue sample.
The kit utilizes paramagnetic bead technology enabling high yields and robust performance. High binding capacity, uniform particle size, and rapid magnetic response of MagJET magnetic beads makes the technology ideal for high throughput automatic nucleic acid purification, as well as for manual purification for low sample throughput.
The resulting high quality DNA is free of proteins, nucleases and other contaminants or inhibitors, and can be used in a wide range of downstream applications such as PCR, qPCR or other enzymatic reactions. See Table 1 for typical genomic DNA yields from various sources.
Highlights
• Versatile—suitable for any plant type and part, including woody, polyphenol-rich and lignified samples
• High gDNA yields—compared to traditional CTAB method
• High quality—isolates high integrity gDNA, free of inhibitors and RNA contamination
• Flexible—easily adjustable for high throughput automation platforms and manual processing
Applications
• Isolated genomic DNA is ideal for use in common molecular biology procedures, including:
• PCR and qPCR FastDigest and conventional restriction digestion
• Southern blotting
The MagJET Plant Genomic DNA Kit procedures are optimized for the isolation of genomic DNA from 10-100 mg plant samples. DNA yields vary between different species and tissues depending on genome size, ploidy, cell number, and age of tissue sample.
The kit utilizes paramagnetic bead technology enabling high yields and robust performance. High binding capacity, uniform particle size, and rapid magnetic response of MagJET magnetic beads makes the technology ideal for high throughput automatic nucleic acid purification, as well as for manual purification for low sample throughput.
The resulting high quality DNA is free of proteins, nucleases and other contaminants or inhibitors, and can be used in a wide range of downstream applications such as PCR, qPCR or other enzymatic reactions. See Table 1 for typical genomic DNA yields from various sources.
Highlights
• Versatile—suitable for any plant type and part, including woody, polyphenol-rich and lignified samples
• High gDNA yields—compared to traditional CTAB method
• High quality—isolates high integrity gDNA, free of inhibitors and RNA contamination
• Flexible—easily adjustable for high throughput automation platforms and manual processing
Applications
• Isolated genomic DNA is ideal for use in common molecular biology procedures, including:
• PCR and qPCR FastDigest and conventional restriction digestion
• Southern blotting
DNA Binding Beads for MagMAX™ DNA Multi-Sample Ultra Kit (Applied Biosystems™)
These are the same DNA Binding Beads found in the MagMAX™ DNA Multi-Sample Ultra Kit (Cat. Nos. A25597, A25598), available separately in case more DNA Binding Beads are required for a particular application.
Bead Tubes for PureLink™ Microbiome DNA Purification Kit (Invitrogen™)
These are the same bead tubes that are included in the PureLink™ Microbiome DNA Purification Kit (Cat. No. A29790), offered separately for additional applications.
PrepFiler Express™ Forensic DNA Extraction Kit (Applied Biosystems™)
This kit is for use with the AutoMate Express™ Forensic DNA Extraction System. It is suitable for the majority of standard sample types encountered in forensic laboratories such as bodily fluids on different substrates including FTA paper, cotton swabs, cotton cloth, denim and many others. We have designed the PrepFiler chemistry specifically to improve the quantity and quality of DNA isolated from forensic samples, thereby increasing the potential to obtain maximum information from downstream Short Tandem Repeat (STR) analysis.
Key Product Features:
• Designed to improve the yield and overall purity of DNA isolated from both routine and challenging forensic samples.
• Provides high quality DNA, free of PCR inhibitors and suitable for downstream applications such as quantitative real-time PCR and short tandem repeat (STR) analysis
• Simple and fast separation of substrate from lysate with the unique PrepFiler LySep™ Column
• Quick and easy run setup with ready-to-use pre-filled cartridges
• Closed system that minimizes the risk of contamination and transposition errors
• Processes 1 to 13 samples in a single run
• Rapid implementation with pre-validated, pre-programmed instrument protocols
• Integrated solution including novel reagents, consumables and automation to provide time saving, cost effective and reliable DNA extraction
• Improves downstream genotyping (STR) success rate and profile quality
Streamlined Off-Line Lysis
The PrepFiler Express™ Kits include the innovative PrepFiler LySep™ Column, which dramatically streamlines the off-line lysis portion of the extraction method. PrepFiler™ Lysis buffer or PrepFiler BTA™ Lysis buffer is added directly into the PrepFiler LySep™ Column along with the sample for incubation. Next, the PrepFiler LySep™ Column is quickly centrifuged which permits lysate flow through the burstable membrane while the substrate remains behind in the column. The sample tube is then simply ready to be placed on the AutoMate Express™ instrument. This eliminates the manual lysate and substrate transfer steps, saving time and minimizing cross contamination and sample transposition events.
For Research, Forensic, or Paternity Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Key Product Features:
• Designed to improve the yield and overall purity of DNA isolated from both routine and challenging forensic samples.
• Provides high quality DNA, free of PCR inhibitors and suitable for downstream applications such as quantitative real-time PCR and short tandem repeat (STR) analysis
• Simple and fast separation of substrate from lysate with the unique PrepFiler LySep™ Column
• Quick and easy run setup with ready-to-use pre-filled cartridges
• Closed system that minimizes the risk of contamination and transposition errors
• Processes 1 to 13 samples in a single run
• Rapid implementation with pre-validated, pre-programmed instrument protocols
• Integrated solution including novel reagents, consumables and automation to provide time saving, cost effective and reliable DNA extraction
• Improves downstream genotyping (STR) success rate and profile quality
Streamlined Off-Line Lysis
The PrepFiler Express™ Kits include the innovative PrepFiler LySep™ Column, which dramatically streamlines the off-line lysis portion of the extraction method. PrepFiler™ Lysis buffer or PrepFiler BTA™ Lysis buffer is added directly into the PrepFiler LySep™ Column along with the sample for incubation. Next, the PrepFiler LySep™ Column is quickly centrifuged which permits lysate flow through the burstable membrane while the substrate remains behind in the column. The sample tube is then simply ready to be placed on the AutoMate Express™ instrument. This eliminates the manual lysate and substrate transfer steps, saving time and minimizing cross contamination and sample transposition events.
For Research, Forensic, or Paternity Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Chargeswitch™ Pro Filter Plasmid Maxi Kit (Invitrogen™)
The Chargeswitch® -Pro Filter Plasmid Maxi Kit includes 10 plasmid maxipreps and provides high yield and high purity plasmid with the fastest and the simplest protocol.
The ChargeSwitch®-Pro Plasmid Filter Mini, Midi, Maxi Kits generates high-quality plasmid DNA without the use of harsh or potentially interfering reagents, for better success in downstream applications. The system is based upon a novel dual⁄nested column design which facilitate the fastest and simplest protocol for high quality plasmid. The system is composed of two columns. The inner flat bottomed column, also referred to as “lysate clarification" column, contains filter materials suitable for rapid clarification of bacterial lysate. The outer luer bottomed column, also referred to as “binding" column, contains ChargeSwitch™ (CST) derivatized membrane that immediately binds plasmid DNA from the clarified lysate. This nested-column design enables simultaneous clarification and DNA binding in a single short centrifugation (or vacuum) step. The combined advantages of novel, optimized plastics and a unique chemistry are an extreme competitive advantage of speed and convenience.
To accomplish this, the ChargeSwitch®-Pro Filter Kit provides:
• 100% ethanol-free, guanidinium-free, organics-free, aqueous protocol for a safer, more streamlined method
• Cleaner purified product for improved performance in downstream applications
• A novel Dual⁄nested column design for simultaneous lysate clearing and binding step which offers the fastest and the simplest protocol
• Endotoxin units < 10 EU ⁄ ug DNA for
• Vacuum Elution can be done with the EveryPrep™ Universal Vacuum Manifold (SKU # K211101)
How it works
The ChargeSwitch® technology replaces the use of ethanol, chaotropic salts, or organic solvents for nucleic acid purification with a simple ion-exchange mechanism. At low pH values (<6.5) the ChargeSwitch® surface ligand is positively charged and binds plasmid DNA. Raising the pH (>8.5) neutralizes the charge and elutes the bound nucleic acid. The ChargeSwitch® protocol retains the ease and familiarity of the common silica spin column protocol with a binding, washing, and elution step, making adapting to the ChargeSwitch® method effortless.
The ChargeSwitch®-Pro Plasmid Filter Mini, Midi, Maxi Kits generates high-quality plasmid DNA without the use of harsh or potentially interfering reagents, for better success in downstream applications. The system is based upon a novel dual⁄nested column design which facilitate the fastest and simplest protocol for high quality plasmid. The system is composed of two columns. The inner flat bottomed column, also referred to as “lysate clarification" column, contains filter materials suitable for rapid clarification of bacterial lysate. The outer luer bottomed column, also referred to as “binding" column, contains ChargeSwitch™ (CST) derivatized membrane that immediately binds plasmid DNA from the clarified lysate. This nested-column design enables simultaneous clarification and DNA binding in a single short centrifugation (or vacuum) step. The combined advantages of novel, optimized plastics and a unique chemistry are an extreme competitive advantage of speed and convenience.
To accomplish this, the ChargeSwitch®-Pro Filter Kit provides:
• 100% ethanol-free, guanidinium-free, organics-free, aqueous protocol for a safer, more streamlined method
• Cleaner purified product for improved performance in downstream applications
• A novel Dual⁄nested column design for simultaneous lysate clearing and binding step which offers the fastest and the simplest protocol
• Endotoxin units < 10 EU ⁄ ug DNA for
• Vacuum Elution can be done with the EveryPrep™ Universal Vacuum Manifold (SKU # K211101)
How it works
The ChargeSwitch® technology replaces the use of ethanol, chaotropic salts, or organic solvents for nucleic acid purification with a simple ion-exchange mechanism. At low pH values (<6.5) the ChargeSwitch® surface ligand is positively charged and binds plasmid DNA. Raising the pH (>8.5) neutralizes the charge and elutes the bound nucleic acid. The ChargeSwitch® protocol retains the ease and familiarity of the common silica spin column protocol with a binding, washing, and elution step, making adapting to the ChargeSwitch® method effortless.
PureLink™ PCR Purification Kit (Invitrogen™)
Le kit de purification de PCR PureLink® permet de supprimer rapidement et efficacement les amorces courtes, les dNTP, les enzymes, les produits de PCR insuffisants et les sels issus des produits PCR. La procédure simple de liaison /lavage /élution PureLink® peut :
• fournir deux tampons de liaison pour les fragments >100 bp ou >300 bp ;
• être effectuée dans une colonne unique ou une plaque de 96 puits ;
• dure <10 min
Utilisation du kit de purification de PCR PureLink®
Deux tampons exclusifs sont fournis pour satisfaire les besoins des diverses contraintes de purification. Utilisez le tampon de liaison pour les purifications de routine des fragments d’ADN double brin de 100 bp à 12 kb. Utilisez le tampon de liaison HC pour supprimer les dimères d’amorces (<300 bp) pour éliminer la tâche laborieuse consistant à purifier le gel. Les produits purifiés de PCR, qui utilisent le kit PureLink®, enregistrent des performances exceptionnelles dans les applications en aval, comme le clonage, le séquençage automatisé d’ADN fluorescent, la PCR, la cartographie des sites de restriction et le marquage.
• fournir deux tampons de liaison pour les fragments >100 bp ou >300 bp ;
• être effectuée dans une colonne unique ou une plaque de 96 puits ;
• dure <10 min
Utilisation du kit de purification de PCR PureLink®
Deux tampons exclusifs sont fournis pour satisfaire les besoins des diverses contraintes de purification. Utilisez le tampon de liaison pour les purifications de routine des fragments d’ADN double brin de 100 bp à 12 kb. Utilisez le tampon de liaison HC pour supprimer les dimères d’amorces (<300 bp) pour éliminer la tâche laborieuse consistant à purifier le gel. Les produits purifiés de PCR, qui utilisent le kit PureLink®, enregistrent des performances exceptionnelles dans les applications en aval, comme le clonage, le séquençage automatisé d’ADN fluorescent, la PCR, la cartographie des sites de restriction et le marquage.
GeneJET Endo-Free Plasmid Maxiprep Kit (Thermo Scientific™)
Thermo Scientific GeneJET Endo-Free Plasmid Maxiprep Kit is a fast, convenient, and efficient system for the large scale purification of endotoxin-free plasmid DNA. Endotoxins are major contaminants found in plasmid DNA preparations. For transfection of sensitive cell lines endotoxins are a major problem. The same applies to in vivo transfection as endotoxins can elicit strong immune responses. Thermo Scientific GeneJET Endo-Free Plasmid Maxiprep Kit is designed to reduce endotoxin level to <0.1 EU/µg of plasmid DNA. The kit utilizes a silica-based membrane technology in the form of convenient spin columns.
Each preparation recovers up to 1000 µg of high copy plasmid DNA from 350 mL of recombinant E. coli cultures that can be used in a wide variety of molecular biology procedures such as transfection of sensitive cells (e.g. Huh-7), in vivo transfection, transformation, restriction endonuclease digestion, automated sequencing, and in vitro transcription.
Highlights
• Pure—free of endotoxins plasmid DNA suitable for transfection of sensitive cells
• Efficient—plasmid recovery resulting in high DNA yields
• Fast—isolation of high quality plasmid DNA in less than 60 minutes
• Convenient—spin column format, centrifuge or vacuum protocol
Applications
• Transfection of sensitive cell lines
• in vivo transfection
• Automated sequencing
• PCR
• Transformation
• in vitro transcription
Includes
• Resuspension Solution
• Lysis Solution
• Neutralization Solution
• Endotoxin Binding Reagent
• Wash Solution I (conc.)
• Wash Solution II (conc.)
• RNase A Solution
• Elution Buffer
• GeneJET Endo-Free Maxi Purification Columns & Collection Tubes (50 mL, blue-capped)
• GeneJET Endo-Free Maxi Filtration Columns & Collection Tubes (50 mL, white-capped)
Each preparation recovers up to 1000 µg of high copy plasmid DNA from 350 mL of recombinant E. coli cultures that can be used in a wide variety of molecular biology procedures such as transfection of sensitive cells (e.g. Huh-7), in vivo transfection, transformation, restriction endonuclease digestion, automated sequencing, and in vitro transcription.
Highlights
• Pure—free of endotoxins plasmid DNA suitable for transfection of sensitive cells
• Efficient—plasmid recovery resulting in high DNA yields
• Fast—isolation of high quality plasmid DNA in less than 60 minutes
• Convenient—spin column format, centrifuge or vacuum protocol
Applications
• Transfection of sensitive cell lines
• in vivo transfection
• Automated sequencing
• PCR
• Transformation
• in vitro transcription
Includes
• Resuspension Solution
• Lysis Solution
• Neutralization Solution
• Endotoxin Binding Reagent
• Wash Solution I (conc.)
• Wash Solution II (conc.)
• RNase A Solution
• Elution Buffer
• GeneJET Endo-Free Maxi Purification Columns & Collection Tubes (50 mL, blue-capped)
• GeneJET Endo-Free Maxi Filtration Columns & Collection Tubes (50 mL, white-capped)
MagJET RNA Purification Kit (Thermo Scientific™)
Thermo Scientific MagJET RNA Kit is designed for fast and efficient purification of total RNA from cultured mammalian cells, tissues, bacteria and yeast.
The kit utilizes paramagnetic bead technology enabling high yields and robust performance. High binding capacity, uniform particle size, and rapid magnetic response of MagJET magnetic beads makes the technology ideal for high throughput automatic nucleic acid purification, as well as for manual nucleic acid purification by low sample throughput users.
The resulting high quality purified RNA is free of proteins, nucleases and other contaminants or inhibitors, can be used in a wide range of downstream applications, such as RT-PCR, RT-qPCR and other enzymatic reactions.
Highlights
• Versatile—suitable for total RNA purification from variety of biological samples, including eukaryotic cells, animal tissues, yeast and bacteria
• Efficient—high yields of high-integrity RNA
• High quality—isolates intact total RNA, free of inhibitors and gDNA contamination
• Flexible—easily adjustable for high throughput automation platforms and manual processing
Applications
• Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:
• RT-PCR
• RT-qPCR
• Northern blotting
• Nuclease protection assay
The kit utilizes paramagnetic bead technology enabling high yields and robust performance. High binding capacity, uniform particle size, and rapid magnetic response of MagJET magnetic beads makes the technology ideal for high throughput automatic nucleic acid purification, as well as for manual nucleic acid purification by low sample throughput users.
The resulting high quality purified RNA is free of proteins, nucleases and other contaminants or inhibitors, can be used in a wide range of downstream applications, such as RT-PCR, RT-qPCR and other enzymatic reactions.
Highlights
• Versatile—suitable for total RNA purification from variety of biological samples, including eukaryotic cells, animal tissues, yeast and bacteria
• Efficient—high yields of high-integrity RNA
• High quality—isolates intact total RNA, free of inhibitors and gDNA contamination
• Flexible—easily adjustable for high throughput automation platforms and manual processing
Applications
• Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:
• RT-PCR
• RT-qPCR
• Northern blotting
• Nuclease protection assay
