Shop All Reverse Transcriptase
SuperScript™ VILO™ Master Mix (Invitrogen™)
SuperScript VILO Master Mix is a first-strand cDNA synthesis master mix for two-step RT-qPCR applications. The master mix combines SuperScript III Reverse Transcriptase (RT) and other RT reaction components in a single-tube format to enable reliable first-strand synthesis and high cDNA yields from a wide range of input RNA concentrations. The high RT efficiency of SuperScript III RT and the optimized formulation allow the master mix to deliver high linearity in cDNA synthesis across a broad range of input RNA template for two-step quantitative RT-PCR (qRT-PCR) assays.
Note: For superior cDNA synthesis performance in RT-qPCR applications, we recommend SuperScript IV VILO Master Mix or SuperScript IV VILO Master Mix with ezDNase Enzyme. SuperScript IV VILO Master Mix elevates the trusted VILO technology to the next level with the highly processive and thermostable SuperScript IV RT and further optimized buffer. The master mix enables efficient cDNA synthesis at higher temperatures in less time and provides superior cDNA yield and sensitivity even with suboptimal purity or scarce templates.
Note: For superior cDNA synthesis performance in RT-qPCR applications, we recommend SuperScript IV VILO Master Mix or SuperScript IV VILO Master Mix with ezDNase Enzyme. SuperScript IV VILO Master Mix elevates the trusted VILO technology to the next level with the highly processive and thermostable SuperScript IV RT and further optimized buffer. The master mix enables efficient cDNA synthesis at higher temperatures in less time and provides superior cDNA yield and sensitivity even with suboptimal purity or scarce templates.
RevertAid Reverse Transcriptase (200 U/µL) (Thermo Scientific™)
Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids which is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase.
Highlights
• Efficient synthesis of full-length first strand cDNA up to 13 kb
• Optimum activity at 42°C
• Active up to 50°C
• Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, fluorescein-labeled nucleotides)
Applications
• First strand cDNA synthesis for RT-PCR and real-time RT-PCR
• Synthesis of cDNA for cloning and expression
• Generation of labeled cDNA probes for microarrays
• DNA labeling
• Analysis of RNA by primer extension
Highlights
• Efficient synthesis of full-length first strand cDNA up to 13 kb
• Optimum activity at 42°C
• Active up to 50°C
• Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, fluorescein-labeled nucleotides)
Applications
• First strand cDNA synthesis for RT-PCR and real-time RT-PCR
• Synthesis of cDNA for cloning and expression
• Generation of labeled cDNA probes for microarrays
• DNA labeling
• Analysis of RNA by primer extension
SuperScript™ IV VILO™ Master Mix (Invitrogen™)
SuperScript™ IV VILO™ (SSIV VILO) Master Mix is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications.
Features of SuperScript IV VILO Master Mix include:
• Super-fast RT reactions in just 10 minutes
• Super-high yields—lower Ct values by more than 2 cycles ahead of all other reverse transcription reagents
• Super-convenient one-tube cDNA reaction master mix for two-step RT-qPCR
• Super-efficient reaction even with low template amounts and suboptimal purity samples
SSIV VILO master mix elevates the trusted VILO technology to the next level with the highly processive and thermostable SuperScript™ IV Reverse Transcriptase and further optimized buffer. These components enable efficient cDNA synthesis at higher temperatures and in less time. SSIV VILO master mix provides superior cDNA yield and sensitivity even with suboptimal purity or scarce templates. It is your new tool for more efficient and reproducible RT-qPCR.
For even better performance, we also offer SuperScript IV VILO Master Mix with ezDNase. The ezDNase further accelerates the RT-qPCR workflow through an extremely simplified genomic DNA removal step. This dramatically reduces the time of the entire reverse transcription protocol and reduces possible variation in gene expression due to RNA loss or damage during conventional DNase treatment.
SSIV VILO master mix gives you more cDNA in less time with less pipeting, less variation, less reaction inhibition, less gDNA interference, and with less sample than all the other cDNA synthesis kits or master mixes for RT-qPCR.
Source
Purified from E. coli expressing the pol gene of M-MLV, modified to increase thermostability and half-life, processivity, inhibitor resistance, and to reduce RNase H activity.
Performance and quality testing
Assayed for endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease, as well as yield and length of cDNA product.
Features of SuperScript IV VILO Master Mix include:
• Super-fast RT reactions in just 10 minutes
• Super-high yields—lower Ct values by more than 2 cycles ahead of all other reverse transcription reagents
• Super-convenient one-tube cDNA reaction master mix for two-step RT-qPCR
• Super-efficient reaction even with low template amounts and suboptimal purity samples
SSIV VILO master mix elevates the trusted VILO technology to the next level with the highly processive and thermostable SuperScript™ IV Reverse Transcriptase and further optimized buffer. These components enable efficient cDNA synthesis at higher temperatures and in less time. SSIV VILO master mix provides superior cDNA yield and sensitivity even with suboptimal purity or scarce templates. It is your new tool for more efficient and reproducible RT-qPCR.
For even better performance, we also offer SuperScript IV VILO Master Mix with ezDNase. The ezDNase further accelerates the RT-qPCR workflow through an extremely simplified genomic DNA removal step. This dramatically reduces the time of the entire reverse transcription protocol and reduces possible variation in gene expression due to RNA loss or damage during conventional DNase treatment.
SSIV VILO master mix gives you more cDNA in less time with less pipeting, less variation, less reaction inhibition, less gDNA interference, and with less sample than all the other cDNA synthesis kits or master mixes for RT-qPCR.
Source
Purified from E. coli expressing the pol gene of M-MLV, modified to increase thermostability and half-life, processivity, inhibitor resistance, and to reduce RNase H activity.
Performance and quality testing
Assayed for endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease, as well as yield and length of cDNA product.
SuperScript™ IV Reverse Transcriptase (Invitrogen™)
La transcriptase inverse (RT) SuperScript® IV est un mutant exclusif du virus de la leucémie murine de Moloney (M-MLV) avec une robustesse et une fiabilité élevées dans les réactions de transcription inverse. Elle affiche une résistance aux inhibiteurs, une processivité et une vitesse de réaction sensiblement améliorées par rapport à SuperScript® III, tout en conservant tous les avantages de l’enzyme précédent, notamment une thermostabilité supérieure, une synthèse d’ADNc très efficace sur toute la longueur et une activité RNase réduite. La RT SuperScript® IV est conçue pour offrir une synthèse rapide, fiable et homogène de l’ADNc, en présence d’inhibiteurs dans un grand nombre d’échantillons, qui empêchent d’autres RT actuellement disponibles d’agir efficacement.
La RT SuperScript® IV constitue le choix de prédilection pour toutes les applications de RT-PCR et RT-qPCR dans la gamme de produits SuperScript®. Nous la recommandons tout particulièrement lorsque la reproductibilité et la fiabilité sont des préoccupations majeures et lorsque les inhibiteurs de l’échantillon d’ARN risquent d’interférer avec la synthèse d’ADNc, conduisant à des résultats biaisés dans les études d’expression génétique.
Caractéristiques de la transcriptase inverse SuperScript® IV :
• Meilleure résistance à toute une variété d’inhibiteurs qui peuvent interférer avec la synthèse d’ADNc
• Synthèse d’ADNc spécifique et robuste dans une large gamme de types d’échantillons
• Réaction catalysée par la transcriptase inverse plus rapide, avec réduction du temps d’incubation qui passe de >50 minutes à 10 minutes
• Processivité bien meilleure par rapport à la RT SuperScript® III
Source
Purifié à partir de E. coli exprimant le gène pol de M-MLV, modifié pour augmenter la thermostabilité et la demi-vie, la processivité, la résistance aux inhibiteurs et pour réduire l’activité RNase H.
Performance et tests de qualité
Dosages d’endodésoxyribonucléase, d’exodésoxyribonucléase et de ribonucléase ; et rendement et longueur du produit d’ADNc.
Définition de l’unité
Une unité de RT SuperScript® IV représente la quantité d’enzyme requise pour incorporer 1 nmole de désoxyribonucléotide dans une substance précipitable à l’acide, en 10 min, à 37°C, en utilisant poly(A) oligo(dT)12-18 comme modèle/amorce.
Conditions de réaction de l’unité
50 mM de Tris-HCl (pH 8,3), 4 mM de MgCl2, 10 mM de DTT, 50 mM de KCl, 0,5 mM de dTTP, 0,4 MBq/ml [3H]-dTTP, 0,4 mM de poly(A) oligo(dT)12-18, et enzyme dans 20 μl pendant 10 min à 37°C.
La RT SuperScript® IV constitue le choix de prédilection pour toutes les applications de RT-PCR et RT-qPCR dans la gamme de produits SuperScript®. Nous la recommandons tout particulièrement lorsque la reproductibilité et la fiabilité sont des préoccupations majeures et lorsque les inhibiteurs de l’échantillon d’ARN risquent d’interférer avec la synthèse d’ADNc, conduisant à des résultats biaisés dans les études d’expression génétique.
Caractéristiques de la transcriptase inverse SuperScript® IV :
• Meilleure résistance à toute une variété d’inhibiteurs qui peuvent interférer avec la synthèse d’ADNc
• Synthèse d’ADNc spécifique et robuste dans une large gamme de types d’échantillons
• Réaction catalysée par la transcriptase inverse plus rapide, avec réduction du temps d’incubation qui passe de >50 minutes à 10 minutes
• Processivité bien meilleure par rapport à la RT SuperScript® III
Source
Purifié à partir de E. coli exprimant le gène pol de M-MLV, modifié pour augmenter la thermostabilité et la demi-vie, la processivité, la résistance aux inhibiteurs et pour réduire l’activité RNase H.
Performance et tests de qualité
Dosages d’endodésoxyribonucléase, d’exodésoxyribonucléase et de ribonucléase ; et rendement et longueur du produit d’ADNc.
Définition de l’unité
Une unité de RT SuperScript® IV représente la quantité d’enzyme requise pour incorporer 1 nmole de désoxyribonucléotide dans une substance précipitable à l’acide, en 10 min, à 37°C, en utilisant poly(A) oligo(dT)12-18 comme modèle/amorce.
Conditions de réaction de l’unité
50 mM de Tris-HCl (pH 8,3), 4 mM de MgCl2, 10 mM de DTT, 50 mM de KCl, 0,5 mM de dTTP, 0,4 MBq/ml [3H]-dTTP, 0,4 mM de poly(A) oligo(dT)12-18, et enzyme dans 20 μl pendant 10 min à 37°C.
RevertAid H Minus Reverse Transcriptase (200 U/µL) (Thermo Scientific™)
Thermo Scientific RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain. RevertAid H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA, and therefore high yields of full-length cDNA from long templates are obtained. RevertAid H Minus Reverse Transcriptase maintains activity over a wide temperature range (42 to 50°C).
Highlights
• High yields of full-length first strand cDNA up to 13 kb
• Optimum activity at 42 to 45°C
• Active up to 55°C
• Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, fluorescein-labeled nucleotides)
Applications
• First strand cDNA synthesis for RT-PCR and real-time RT-PCR
• Reverse transcription at elevated temperatures to reduce effects of secondary structure
• Synthesis of cDNA for cloning and expression
• Generation of labeled cDNA probes for microarrays
• DNA labeling
• Analysis of RNA by primer extension
Related Products
RevertAid H Minus Reverse Transcriptase (200 U/µL)
Highlights
• High yields of full-length first strand cDNA up to 13 kb
• Optimum activity at 42 to 45°C
• Active up to 55°C
• Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, fluorescein-labeled nucleotides)
Applications
• First strand cDNA synthesis for RT-PCR and real-time RT-PCR
• Reverse transcription at elevated temperatures to reduce effects of secondary structure
• Synthesis of cDNA for cloning and expression
• Generation of labeled cDNA probes for microarrays
• DNA labeling
• Analysis of RNA by primer extension
Related Products
RevertAid H Minus Reverse Transcriptase (200 U/µL)
M-MLV Reverse Transcriptase (100 U/µL) (Invitrogen™)
M-MLV Reverse Transcriptase (RT) is commonly used in the first step of RT-PCR as well as for primer extension experiments. In the presence of a single-stranded RNA or DNA template, primer, and deoxyribonucleotides, this enzyme will extend the oligonucleotide primer, producing a complementary strand.
Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is derived from a cloned region of the pol gene of M-MLV and isolated from an E. coli strain overexpressing this construct.
Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is derived from a cloned region of the pol gene of M-MLV and isolated from an E. coli strain overexpressing this construct.
SuperScript™ IV VILO™ Master Mix with ezDNase™ Enzyme (Invitrogen™)
SuperScript™ IV VILO™ (SSIV VILO) Master Mix is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications. Inclusion of ezDNase™ enzyme further accelerates the RT-qPCR workflow through an extremely simplified genomic DNA removal step. (This master mix is also available without ezDNase.)
Features of SuperScript IV VILO Master Mix include:
• Super-fast RT reaction in just 10 minutes and gDNA removal in 2 minutes
• Super-high yields—lower Ct values by more than 2 cycles ahead of all other reverse transcription reagents
• Super-convenient one-tube cDNA reaction master mix for two-step RT-qPCR
• Super-efficient reaction even with low template amounts and suboptimal purity samples
SSIV VILO master mix elevates the trusted VILO technology to the next level with the highly processive and thermostable SuperScript™ IV Reverse Transcriptase and further optimized buffer. These components enable efficient cDNA synthesis at higher temperatures and in less time. SSIV VILO master mix provides superior cDNA yield and sensitivity even with suboptimal purity or scarce templates. It is your new tool for more efficient and reproducible RT-qPCR.
For even better performance, this SuperScript IV VILO Master Mix includes ezDNase (also available as a separate purchase) for genomic DNA removal to further accelerate the RT-qPCR workflow. The extremely simplified genomic DNA removal step dramatically reduces the time of the entire reverse transcription protocol and reduces possible variation in gene expression due to RNA loss or damage during conventional DNase treatment.
SSIV VILO master mix gives you more cDNA in less time with less pipeting, less variation, less reaction inhibition, less gDNA interference, and with less sample than all the other cDNA synthesis kits or master mixes for RT-qPCR.
Source
Purified from E. coli expressing the pol gene of M-MLV, modified to increase thermostability and half-life, processivity, inhibitor resistance, and to reduce RNase H activity.
Performance and quality testing
Assayed for endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease, as well as yield and length of cDNA product.
Features of SuperScript IV VILO Master Mix include:
• Super-fast RT reaction in just 10 minutes and gDNA removal in 2 minutes
• Super-high yields—lower Ct values by more than 2 cycles ahead of all other reverse transcription reagents
• Super-convenient one-tube cDNA reaction master mix for two-step RT-qPCR
• Super-efficient reaction even with low template amounts and suboptimal purity samples
SSIV VILO master mix elevates the trusted VILO technology to the next level with the highly processive and thermostable SuperScript™ IV Reverse Transcriptase and further optimized buffer. These components enable efficient cDNA synthesis at higher temperatures and in less time. SSIV VILO master mix provides superior cDNA yield and sensitivity even with suboptimal purity or scarce templates. It is your new tool for more efficient and reproducible RT-qPCR.
For even better performance, this SuperScript IV VILO Master Mix includes ezDNase (also available as a separate purchase) for genomic DNA removal to further accelerate the RT-qPCR workflow. The extremely simplified genomic DNA removal step dramatically reduces the time of the entire reverse transcription protocol and reduces possible variation in gene expression due to RNA loss or damage during conventional DNase treatment.
SSIV VILO master mix gives you more cDNA in less time with less pipeting, less variation, less reaction inhibition, less gDNA interference, and with less sample than all the other cDNA synthesis kits or master mixes for RT-qPCR.
Source
Purified from E. coli expressing the pol gene of M-MLV, modified to increase thermostability and half-life, processivity, inhibitor resistance, and to reduce RNase H activity.
Performance and quality testing
Assayed for endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease, as well as yield and length of cDNA product.
M-MLV Reverse Transcriptase (200 U/µL) (Invitrogen™)
La transcriptase inverse M-MLV est un ADN polymérase recombinant qui synthétise un brin d’ADN complémentaire à partir d’ARN monocaténaire, d’ADN ou d’ARN /ADN hybride. Comparée à AMV RT, la transcriptase inverse Moloney Murine Leukemia Virus (M-MLV RT) est dépourvue de toute activité de l’endonucléase d’ADN et affiche une activité RNase H inférieure. Fonctions de cette enzyme :
• Thermostabilité : activité optimale à 37°C
• Dimensions de l’ADNc : M-MLV peut servir à synthétiser l’ADNc monocaténaire jusqu’à 7 kb
• Applications : synthèse d’ADNc monocaténaire, extension de l’amorce, ADNdb de séquençage, bibliothèques d’ADNc et RT-PCR
Source
Forme purifiée de l’E. coli exprimant le gène pol de M-MLV sur plasmide
Performance et tests de qualité
Pureté SDS-PAGE ; dosages d’endodésoxyribonucléase, d’exodésoxyribonucléase et de ribonucléase ; rendement et longueur du produit d’ADNc
Définition de l’unité
Une unité de M-MLV RT représente la quantité d’enzyme requise pour incorporer 1 nmole de désoxyribonucléotide dans une substance précipitable à l’acide, en 10 minutes, à 37°C, en utilisant poly(A) oligo(dT)25 comme modèle/amorce.
Conditions de réaction de l’unité
50 mM de Tris-HCl (pH 8,3), 40 mM de KCl, 6 mM de MgCl2, 1 mM de DTT, 0,5 mM [3H] de dTTP, 0,1 mM de poly(A), 0,1 mM d’oligo(dT)25, 0,1 mg/ml de BSA, et enzyme dans 50 µl pour 10 minutes à 37°C.
• Thermostabilité : activité optimale à 37°C
• Dimensions de l’ADNc : M-MLV peut servir à synthétiser l’ADNc monocaténaire jusqu’à 7 kb
• Applications : synthèse d’ADNc monocaténaire, extension de l’amorce, ADNdb de séquençage, bibliothèques d’ADNc et RT-PCR
Source
Forme purifiée de l’E. coli exprimant le gène pol de M-MLV sur plasmide
Performance et tests de qualité
Pureté SDS-PAGE ; dosages d’endodésoxyribonucléase, d’exodésoxyribonucléase et de ribonucléase ; rendement et longueur du produit d’ADNc
Définition de l’unité
Une unité de M-MLV RT représente la quantité d’enzyme requise pour incorporer 1 nmole de désoxyribonucléotide dans une substance précipitable à l’acide, en 10 minutes, à 37°C, en utilisant poly(A) oligo(dT)25 comme modèle/amorce.
Conditions de réaction de l’unité
50 mM de Tris-HCl (pH 8,3), 40 mM de KCl, 6 mM de MgCl2, 1 mM de DTT, 0,5 mM [3H] de dTTP, 0,1 mM de poly(A), 0,1 mM d’oligo(dT)25, 0,1 mg/ml de BSA, et enzyme dans 50 µl pour 10 minutes à 37°C.
SuperScript™ II Reverse Transcriptase (Invitrogen™)
Invitrogen SuperScript II Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) with reduced RNase H activity and increased thermal stability compared to wild-type MMLV RT. Mutations in the RNase H domain of the enzyme eliminate degradation of the RNA during first-strand cDNA synthesis, which results in higher yields of full-length cDNA.
SuperScript RTs are the most highly trusted and widely used RTs with over 50,000 citations, reviews, and publications to date.
Note: The latest member of the SuperScript RT family, SuperScript IV Reverse Transcriptase, features enhanced thermostability, processivity, yields, and performance with any RNA samples, including those of suboptimal purity or integrity.
SuperScript RTs are the most highly trusted and widely used RTs with over 50,000 citations, reviews, and publications to date.
Note: The latest member of the SuperScript RT family, SuperScript IV Reverse Transcriptase, features enhanced thermostability, processivity, yields, and performance with any RNA samples, including those of suboptimal purity or integrity.
Maxima Reverse Transcriptase (200 U/µL) (Thermo Scientific™)
Thermo Scientific Maxima Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity as well as RNase H activity. The engineered enzyme features dramatically improved thermostability and robustness, and increased synthesis rates compared to wild type M-MuLV RT.
Maxima Reverse Transcriptase is capable of reproducible cDNA synthesis from a wide range of input total RNA amounts (from 1 pg to 5 µg) at elevated temperatures (50 to 65°C), which makes this enzyme an ideal tool for two step RT-qPCR (see supporting data).
Due to its high thermostability, Maxima enzyme maintains full activity during the entire reverse transcription reaction, generates the highest yields of cDNA, and is able to synthesize even very long RNA transcripts up to 20 kb. The reaction temperature can be increased up to 65°C for efficient transcription of RNA regions with a high secondary structure or to improve specificity using gene specific primers. Due to its increased synthesis rate, the reverse transcriptase reaction can be completed in 15 to 30 min.
Highlights
• High yields of full-length cDNA up to 20 kb
• Active up to 65°C
• Thermostable—90% active after incubation at 50°C for 60 minutes
• High sensitivity—reproducible cDNA synthesis from a wide range of total RNA quantities (1 pg to 5 µg)
• Efficient—complete cDNA synthesis in 15 to 30 minutes
• Incorporates modified nucleotides
Applications
• Two step RT-PCR
• Two step RT-qPCR
• First strand cDNA synthesis
• Construction of full length cDNA libraries
• DNA labeling
• Primer extension
Also available: Maxima First Strand cDNA Synthesis Kit for RT-qPCR
Maxima Reverse Transcriptase is capable of reproducible cDNA synthesis from a wide range of input total RNA amounts (from 1 pg to 5 µg) at elevated temperatures (50 to 65°C), which makes this enzyme an ideal tool for two step RT-qPCR (see supporting data).
Due to its high thermostability, Maxima enzyme maintains full activity during the entire reverse transcription reaction, generates the highest yields of cDNA, and is able to synthesize even very long RNA transcripts up to 20 kb. The reaction temperature can be increased up to 65°C for efficient transcription of RNA regions with a high secondary structure or to improve specificity using gene specific primers. Due to its increased synthesis rate, the reverse transcriptase reaction can be completed in 15 to 30 min.
Highlights
• High yields of full-length cDNA up to 20 kb
• Active up to 65°C
• Thermostable—90% active after incubation at 50°C for 60 minutes
• High sensitivity—reproducible cDNA synthesis from a wide range of total RNA quantities (1 pg to 5 µg)
• Efficient—complete cDNA synthesis in 15 to 30 minutes
• Incorporates modified nucleotides
Applications
• Two step RT-PCR
• Two step RT-qPCR
• First strand cDNA synthesis
• Construction of full length cDNA libraries
• DNA labeling
• Primer extension
Also available: Maxima First Strand cDNA Synthesis Kit for RT-qPCR
Maxima H Minus Reverse Transcriptase (200 U/µL) (Thermo Scientific™)
Thermo Scientific Maxima H Minus Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity but lacks RNase H activity due to mutation in RNase H domain of M-MuLV RT. The engineered enzyme features dramatically improved thermostability, 50X higher processivity, robustness and increased synthesis rate compared to wild type M-MuLV RT.
The eliminated RNase H activity enables the enzyme to produce very long RNA transcripts up to 20 kb. Due to its high thermostability, the enzyme maintains full activity during the entire reverse transcription reaction and generates high yields of cDNA. The reaction temperature can be increased up to 65°C for efficient transcription of RNA regions with a high secondary structure or to improve specificity using gene specific primers. The extremely high processivity of Maxima H Minus enzyme results in increased resistance to common reaction inhibitors, such as guanidine, formamide and ethanol.
Highlights
• Thermostable—90% active after incubation at 50°C for 60 minutes in a reaction mixture.
• Active up to 65°C
• High yields of full-length cDNA up to 20 kb
• High sensitivity—reproducible cDNA synthesis from a wide range of starting total RNA amounts (1 pg to 5 µg)
• Efficient—complete cDNA synthesis in 15 to 30 minutes
• Increased resistance to common reaction inhibitors
• Incorporates modified nucleotides
Applications
• First strand cDNA synthesis for RT-PCR and real-time RT-PCR.
• Reverse transcription at elevated temperatures to reduce effects of secondary structure.
• Synthesis of cDNA for cloning and expression.
• Generation of labeled cDNA probes for microarrays.
• Analysis of RNA by primer extension.
Note
• Also available: : Maxima H Minus First Strand cDNA Synthesis Kit.
The eliminated RNase H activity enables the enzyme to produce very long RNA transcripts up to 20 kb. Due to its high thermostability, the enzyme maintains full activity during the entire reverse transcription reaction and generates high yields of cDNA. The reaction temperature can be increased up to 65°C for efficient transcription of RNA regions with a high secondary structure or to improve specificity using gene specific primers. The extremely high processivity of Maxima H Minus enzyme results in increased resistance to common reaction inhibitors, such as guanidine, formamide and ethanol.
Highlights
• Thermostable—90% active after incubation at 50°C for 60 minutes in a reaction mixture.
• Active up to 65°C
• High yields of full-length cDNA up to 20 kb
• High sensitivity—reproducible cDNA synthesis from a wide range of starting total RNA amounts (1 pg to 5 µg)
• Efficient—complete cDNA synthesis in 15 to 30 minutes
• Increased resistance to common reaction inhibitors
• Incorporates modified nucleotides
Applications
• First strand cDNA synthesis for RT-PCR and real-time RT-PCR.
• Reverse transcription at elevated temperatures to reduce effects of secondary structure.
• Synthesis of cDNA for cloning and expression.
• Generation of labeled cDNA probes for microarrays.
• Analysis of RNA by primer extension.
Note
• Also available: : Maxima H Minus First Strand cDNA Synthesis Kit.
SuperScript™ III Reverse Transcriptase (Invitrogen™)
Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) that was created by introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability. SuperScript III RT offers higher cDNA yields, improved cDNA lengths, improved efficiency on GC-rich target RNAs, and overall better performance than wild-type MMLV and MMLV RNase H-minus enzymes.
SuperScript RTs are the most highly trusted and widely used RTs with over 50,000 citations, reviews, and publications to date.
Note: The latest member of the SuperScript RT family, SuperScript IV Reverse Transcriptase, features enhanced thermostability, processivity, yields, and performance with any RNA samples, including those of suboptimal purity or integrity.
SuperScript RTs are the most highly trusted and widely used RTs with over 50,000 citations, reviews, and publications to date.
Note: The latest member of the SuperScript RT family, SuperScript IV Reverse Transcriptase, features enhanced thermostability, processivity, yields, and performance with any RNA samples, including those of suboptimal purity or integrity.
MultiScribe™ Reverse Transcriptase (Invitrogen™)
La transcriptase inverse MultiScribe™ est une transcriptase inverse recombinante du virus de la leucémie murine de Moloney (rMoMuLV), qui a été optimisée pour les dosages TaqMan. La transcriptase inverse MultiScribe™ ressemble à la transcriptase inverse MuLV, en dépit de recommandations d’utilisation divergentes. La transcriptase inverse MultiScribe™ est une ADN polymérase recombinante, dépendant de l’ARN, qui utilise l’ARN monocaténaire pour modèle en présence d’une amorce pour synthétiser un brin d’ADN complémentaire (ADNc). 5 000 U sont fournis dans des flacons de 100 µl (50 U/µl). Avantages de la transcriptase inverse MultiScribe™ :
• Compatible avec les réactions de PCR en temps réel en une ou deux étapes, ce qui facilite la configuration des dosages
• Les composants individuels sont conçus pour être polyvalents dans la configuration des dosages
• Compatible avec les réactions de PCR en temps réel en une ou deux étapes, ce qui facilite la configuration des dosages
• Les composants individuels sont conçus pour être polyvalents dans la configuration des dosages
MuLV Reverse Transcriptase (Invitrogen™)
MuLV Reverse Transcriptase is a recombinant RNA-dependent DNA polymerase that uses single-stranded RNA as a template in the presence of a primer to synthesize a complementary DNA (cDNA) strand. It can be used for the preparation of cDNA libraries, for first-strand synthesis in RT-PCR, or in dideoxy sequencing reactions in place of Klenow enzyme, for G+C–rich regions. MuLV Reverse Transcriptase lacks endonuclease activity and has extremely low RNase H activity compared to AMV reverse transcriptase.
