Shop All PCR Enzymes & Kits
AmpliTaq Gold™ Fast PCR Master Mix (Applied Biosystems™)
The Applied Biosystems® AmpliTaq Gold® Fast PCR Master Mix is a premix, hot start chemistry for end-point PCR analysis. Its optimized chemical design enables to shorten PCR running time from a traditional 2 hours to proximal 40 minutes. It generates high quality PCR products that give high quality sequencing results that customers expect from Applied Biosystems®. Optimized for use in sequencing applications, this kit provides specific, high-yield amplicons that can be easily and robustly sequenced. This premixed master mix works with your existing primers to provide sensitive, specific and reproducible results.
• Fast Results: Speeds the time from sample to results. Amplifies 600bp length gene in approximately 45 minutes
• Better Sequencing quality: Specific, high-yield amplicons suitable for sequencing applications that yield low peak-under-peak, longer continous read-length and higher QV-30 count
• Easy to use: The 2X master mix allows for quick experiment setup
• No primer annealing restrictions
Speed Time to Discovery
AmpliTaq Gold® Fast PCR Master Mix, UP brings PCR reaction times down to as little as 45 minutes and delivers robust sequencing data on par with standard PCR reagents such as the classic AmpliTaq Gold® PCR Master Mix. This new master mix has been designed for use with your existing primers and results in specific, high-yield amplicons for easy, high quality sequencing data. Cycle sequencing can now also be performed in as little as 50 minutes.
Quick Setup, High Sensitivity
This pre-mixed hotstart kit allows you to setup your experiment quickly without sacrificing performance. This kit provides reproducible, sensitive results, allowing you to detect a single copy of a gene in 10 ng of DNA.
Robust Fast Sequencing Results
Get the sequencing results you expect from Applied Biosystems®. The AmpliTaq Gold® Fast PCR Master Mix, UP is optimized for your sequencing reactions. This kit yields specific high-yield amplicons that provide you with clean sequencing reads including low peak-under-peak, longer read-lengths and superior Q30.
Note: See user's manual or package insert for limited label license, and trademark information. For Research Use Only. Not for use in diagnostics procedures.
• Fast Results: Speeds the time from sample to results. Amplifies 600bp length gene in approximately 45 minutes
• Better Sequencing quality: Specific, high-yield amplicons suitable for sequencing applications that yield low peak-under-peak, longer continous read-length and higher QV-30 count
• Easy to use: The 2X master mix allows for quick experiment setup
• No primer annealing restrictions
Speed Time to Discovery
AmpliTaq Gold® Fast PCR Master Mix, UP brings PCR reaction times down to as little as 45 minutes and delivers robust sequencing data on par with standard PCR reagents such as the classic AmpliTaq Gold® PCR Master Mix. This new master mix has been designed for use with your existing primers and results in specific, high-yield amplicons for easy, high quality sequencing data. Cycle sequencing can now also be performed in as little as 50 minutes.
Quick Setup, High Sensitivity
This pre-mixed hotstart kit allows you to setup your experiment quickly without sacrificing performance. This kit provides reproducible, sensitive results, allowing you to detect a single copy of a gene in 10 ng of DNA.
Robust Fast Sequencing Results
Get the sequencing results you expect from Applied Biosystems®. The AmpliTaq Gold® Fast PCR Master Mix, UP is optimized for your sequencing reactions. This kit yields specific high-yield amplicons that provide you with clean sequencing reads including low peak-under-peak, longer read-lengths and superior Q30.
Note: See user's manual or package insert for limited label license, and trademark information. For Research Use Only. Not for use in diagnostics procedures.
Phire Animal Tissue Direct PCR Kit (without sampling tools) (Thermo Scientific™)
Le kit de PCR directe à partir de tissus animaux Thermo Scientific Phire a été développé pour l’amplification d’ADN directement à partir d’un large éventail de tissus animaux, notamment les souris, les poissons, les oiseaux et les insectes. Le kit contient tous les composants nécessaires pour l’amplification d’ADN directement à partir de tissus animaux : réactifs optimisés pour PCR, tampon de dilution et additif DNARelease, qui peuvent être utilisés pour améliorer la libération d’ADN à partir de tissus animaux. De plus, le kit inclut des amorces de contrôle pour les réactions de contrôle positif qui sont universelles et fonctionnent avec de nombreuses espèces animales. Un manuel détaillé est fourni, qui décrit les protocoles lorsque vous travaillez avec différents types de tissus.
Points clés :
• Pas besoin d’étapes de purification d’ADN chronophages et coûteuses
• Très peu de matériau d’échantillon requis
• Deux protocoles simples pour diverses applications
• L’ADN polymérase Phire Hot Start II offre des rendements élevés de produit spécifique avec un temps d’extension court (20 s/kb).
La température d’hybridation optimale pour l’ADN polymérase Phire peut différer significativement de celle des polymérases à base de Taq.
Pour des résultats optimaux, commencez par calculer de manière précise votre Tm à l’aide de notre calculatrice de Tm.
Points clés :
• Pas besoin d’étapes de purification d’ADN chronophages et coûteuses
• Très peu de matériau d’échantillon requis
• Deux protocoles simples pour diverses applications
• L’ADN polymérase Phire Hot Start II offre des rendements élevés de produit spécifique avec un temps d’extension court (20 s/kb).
La température d’hybridation optimale pour l’ADN polymérase Phire peut différer significativement de celle des polymérases à base de Taq.
Pour des résultats optimaux, commencez par calculer de manière précise votre Tm à l’aide de notre calculatrice de Tm.
Phusion High-Fidelity PCR Master Mix with HF Buffer (Thermo Scientific™)
Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase.
Phusion High-Fidelity PCR Master Mix is convenient 2X mix containing Phusion DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2. Two master mix formulations are available - with HF Buffer (F-531S and F-531L) and with GC Buffer (F-532S and F-532L). The error rate of Phusion DNA Polymerase in HF Buffer (4.4 × 10-7) is lower than that in GC Buffer (9.5 × 10-7). Therefore, the master mix with HF Buffer should be used as the default master mix for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures.
Highlights
• High fidelity (50X Taq)
• Fast PCR due to short extension times (15-30 s/kb)
• Robust performance, minimal optimization needed
Applications
• High-fidelity PCR
• Amplification of difficult (GC-rich) templates
• Cloning
• Template generation for sequencing
• Long-range PCR (up to 20 kb)
• Mutagenesis
• High throughput PCR
Phusion High-Fidelity PCR Master Mix is convenient 2X mix containing Phusion DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2. Two master mix formulations are available - with HF Buffer (F-531S and F-531L) and with GC Buffer (F-532S and F-532L). The error rate of Phusion DNA Polymerase in HF Buffer (4.4 × 10-7) is lower than that in GC Buffer (9.5 × 10-7). Therefore, the master mix with HF Buffer should be used as the default master mix for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures.
Highlights
• High fidelity (50X Taq)
• Fast PCR due to short extension times (15-30 s/kb)
• Robust performance, minimal optimization needed
Applications
• High-fidelity PCR
• Amplification of difficult (GC-rich) templates
• Cloning
• Template generation for sequencing
• Long-range PCR (up to 20 kb)
• Mutagenesis
• High throughput PCR
Taq DNA Polymerase, recombinant (Invitrogen™)
Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. The enzyme consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5´→3´ DNA polymerase activity and a 5´→3´ exonuclease activity.
With our Taq DNA Polymerase, you get:
• Your choice of recombinant or native enzyme
• Amplification of PCR products up to 5 kb in size
• An enzyme that is licensed and qualified for PCR
Applications
Taq DNA Polymerase is appropriate for use in the amplification of DNA from complex genomic, viral, and plasmid templates, RT-PCR, sequencing ssDNA, and cycle sequencing.
Source
Recombinant enzyme is purified from the cloned Thermus aquaticus DNA polymerase gene expressed in E. coli.
Unit definition
One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.
With our Taq DNA Polymerase, you get:
• Your choice of recombinant or native enzyme
• Amplification of PCR products up to 5 kb in size
• An enzyme that is licensed and qualified for PCR
Applications
Taq DNA Polymerase is appropriate for use in the amplification of DNA from complex genomic, viral, and plasmid templates, RT-PCR, sequencing ssDNA, and cycle sequencing.
Source
Recombinant enzyme is purified from the cloned Thermus aquaticus DNA polymerase gene expressed in E. coli.
Unit definition
One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.
AmpliTaq Gold™ DNA Polymerase with Gold Buffer and MgCl2 (Applied Biosystems™)
AmpliTaq Gold® DNA Polymerase is a chemically modified form of AmpliTaq® DNA Polymerase requiring thermal activation. Features of this enzyme:
• Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield
• Time-released thermal activation improves sensitivity in low copy number amplifications
• Successful multiplex PCR saves time and reagents
• Includes GeneAmp® 10X PCR Gold Buffer with MgCl2
Hot-start activation
The modified enzyme is provided in an inactive state. Upon thermal activation, the modifier is permanently released, regenerating active enzyme. The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional PCR techniques.
Higher specificity, higher yield
AmpliTaq Gold® DNA Polymerase can be activated partially or completely in a pre-PCR heat step, or can be allowed to activate slowly in a time-released manner during the denaturation steps of thermal cycling. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity. The yield of specific product increases because reactants are not wasted in the formation of unintended products. Because AmpliTaq Gold® DNA Polymerase is a chemical hot-start enzyme, there is no worry of biological contamination.
GeneAmp® 10X PCR Gold Buffer is formulated to provide flexible, efficient activation of AmpliTaq Gold® DNA Polymerase, resulting in a highly specific, robust PCR amplification. The ionic strength and the pH of GeneAmp® 10X PCR Gold Buffer have been optimized to provide a wider activation temperature and time range when used in conjunction with AmpliTaq Gold® DNA Polymerase.
AmpliTaq Gold® 360 DNA Polymerase for superior performance
Also available is AmpliTaq Gold® 360 DNA Polymerase for even better PCR performance. Compared to AmpliTaq Gold® DNA Polymerase, AmpliTaq Gold® 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation. When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme, in addition to its hot-start capabilities, reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates. Also included with this product is a 360 GC Enhancer to work through challenging GC-rich templates.
• Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield
• Time-released thermal activation improves sensitivity in low copy number amplifications
• Successful multiplex PCR saves time and reagents
• Includes GeneAmp® 10X PCR Gold Buffer with MgCl2
Hot-start activation
The modified enzyme is provided in an inactive state. Upon thermal activation, the modifier is permanently released, regenerating active enzyme. The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional PCR techniques.
Higher specificity, higher yield
AmpliTaq Gold® DNA Polymerase can be activated partially or completely in a pre-PCR heat step, or can be allowed to activate slowly in a time-released manner during the denaturation steps of thermal cycling. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity. The yield of specific product increases because reactants are not wasted in the formation of unintended products. Because AmpliTaq Gold® DNA Polymerase is a chemical hot-start enzyme, there is no worry of biological contamination.
GeneAmp® 10X PCR Gold Buffer is formulated to provide flexible, efficient activation of AmpliTaq Gold® DNA Polymerase, resulting in a highly specific, robust PCR amplification. The ionic strength and the pH of GeneAmp® 10X PCR Gold Buffer have been optimized to provide a wider activation temperature and time range when used in conjunction with AmpliTaq Gold® DNA Polymerase.
AmpliTaq Gold® 360 DNA Polymerase for superior performance
Also available is AmpliTaq Gold® 360 DNA Polymerase for even better PCR performance. Compared to AmpliTaq Gold® DNA Polymerase, AmpliTaq Gold® 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation. When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme, in addition to its hot-start capabilities, reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates. Also included with this product is a 360 GC Enhancer to work through challenging GC-rich templates.
AmpliTaq Gold™ DNA Polymerase with Buffer II and MgCl2 (Applied Biosystems™)
AmpliTaq Gold® DNA Polymerase is a chemically modified form of AmpliTaq® DNA Polymerase requiring thermal activation. Features of this enzyme:
• Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield
• Time-released thermal activation improves sensitivity in low copy number amplifications
• Successful multiplex PCR saves time and reagents
• Includes GeneAmp® 10X PCR Buffer II with MgCl2
Hot-start activation
The modified enzyme is provided in an inactive state. Upon thermal activation, the modifier is permanently released, regenerating active enzyme. The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional PCR techniques.
Higher specificity, higher yield
AmpliTaq Gold® DNA Polymerase can be activated partially or completely in a pre-PCR heat step, or can be allowed to activate slowly in a time-released manner during the denaturation steps of thermal cycling. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity. The yield of specific product increases because reactants are not wasted in the formation of unintended products. Because AmpliTaq Gold® DNA Polymerase is a chemical hot-start enzyme, there is no worry of biological contamination.
AmpliTaq Gold® 360 DNA Polymerase for superior performance
Also available is AmpliTaq Gold® 360 DNA Polymerase for even better PCR performance. Compared to AmpliTaq Gold® DNA Polymerase, AmpliTaq Gold® 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation. When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme, in addition to its hot-start capabilities, reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates. Also included with this product is a 360 GC Enhancer to work through challenging GC-rich templates.
• Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield
• Time-released thermal activation improves sensitivity in low copy number amplifications
• Successful multiplex PCR saves time and reagents
• Includes GeneAmp® 10X PCR Buffer II with MgCl2
Hot-start activation
The modified enzyme is provided in an inactive state. Upon thermal activation, the modifier is permanently released, regenerating active enzyme. The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional PCR techniques.
Higher specificity, higher yield
AmpliTaq Gold® DNA Polymerase can be activated partially or completely in a pre-PCR heat step, or can be allowed to activate slowly in a time-released manner during the denaturation steps of thermal cycling. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity. The yield of specific product increases because reactants are not wasted in the formation of unintended products. Because AmpliTaq Gold® DNA Polymerase is a chemical hot-start enzyme, there is no worry of biological contamination.
AmpliTaq Gold® 360 DNA Polymerase for superior performance
Also available is AmpliTaq Gold® 360 DNA Polymerase for even better PCR performance. Compared to AmpliTaq Gold® DNA Polymerase, AmpliTaq Gold® 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation. When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme, in addition to its hot-start capabilities, reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates. Also included with this product is a 360 GC Enhancer to work through challenging GC-rich templates.
Maxima Hot Start PCR Master Mix (2X) (Thermo Scientific™)
Thermo Scientific Maxima Hot Start PCR Master Mix (2X) is a ready-to-use solution containing chemically modified Maxima Hot Start Taq DNA Polymerase, optimized hot start PCR buffer, Mg2+, and dNTPs. The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer dimers and providing higher specificity of DNA amplification. The functional activity of the enzyme is restored during a short 4 minute incubation at 95°C. The activated enzyme maintains the same functionality as Taq DNA polymerase. Highlights
• Convenient—Maxima Hot Start Taq DNA Polymerase in a ready-to-use mix
• Four minute activation time
• High PCR specificity and sensitivity
• Room temperature PCR set up
Applications
• Hot Start PCR
• Routine PCR
• High throughput PCR
• Multiplex PCR
• Genotyping
• Convenient—Maxima Hot Start Taq DNA Polymerase in a ready-to-use mix
• Four minute activation time
• High PCR specificity and sensitivity
• Room temperature PCR set up
Applications
• Hot Start PCR
• Routine PCR
• High throughput PCR
• Multiplex PCR
• Genotyping
AccuPrime™ GC-Rich DNA Polymerase (Invitrogen™)
AccuPrime™ GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content. The kit offers a choice of buffers for amplifying genomic DNA targets (Buffer A) or non-GC-rich cDNA, plasmid, and lambda-based targets (Buffer B). Benefits of using AccuPrime™ GC-Rich DNA Polymerase:
• Yield—high yields for targets up to 5 kb in length
• Specificity—AccuPrime™ accessory proteins for improved PCR specificity
• Sensitivity—sensitivity down to 5 ng of template DNA
Robust and specific amplifications
The AccuPrime™ buffers contain thermostable proteins that enhance primer-template hybridization during PCR, increasing the specificity of the reaction. The polymerase itself, from the archaebacterium Pyrolobus fumarius, has a five-fold better processivity than Taq DNA polymerase, and remains active even after 4 hours at 95°C.
Unit definition
One unit of AccuPrime™ GC-Rich DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of dNTPs into acid-insoluble material in 30 min at 74°C.
• Yield—high yields for targets up to 5 kb in length
• Specificity—AccuPrime™ accessory proteins for improved PCR specificity
• Sensitivity—sensitivity down to 5 ng of template DNA
Robust and specific amplifications
The AccuPrime™ buffers contain thermostable proteins that enhance primer-template hybridization during PCR, increasing the specificity of the reaction. The polymerase itself, from the archaebacterium Pyrolobus fumarius, has a five-fold better processivity than Taq DNA polymerase, and remains active even after 4 hours at 95°C.
Unit definition
One unit of AccuPrime™ GC-Rich DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of dNTPs into acid-insoluble material in 30 min at 74°C.
GeneAmp™ Fast PCR Master Mix (2X) (Applied Biosystems™)
The GeneAmp® Fast PCR Master Mix, with its sensitive, pre-mixed hot-start chemistries, reduces PCR reaction time to as little as 25 minutes when used with the Veriti® 96-Well Thermal Cycler. Features of this master mix:
• Set up reactions quickly, using premixed reagents
• Detect a single copy of a gene in as little as 10 ng of DNA
• Get reproducible, specific, and sensitive results
Easy to use
Pre-mixed, hot-start chemistries enable you to set up your experiments quickly. And by generating greater than 90% "A" overhangs—it makes cloning easier too.
Detect a single copy in 10 ng of DNA
Use the GeneAmp® Fast PCR Master Mix to detect a single copy of a gene in 10 ng of human genomic DNA or as little as 10 copies of an HIV target.
Part of the Applied Biosystems® Fast PCR System
The Applied Biosystems® Fast PCR System, consisting of the premixed GeneAmp® Fast PCR Master Mix, specially designed microplates and tubes, and the Veriti® 96-Well Thermal Cycler (0.1 mL or 0.2 mL), considerably reduces PCR reaction times.
• Set up reactions quickly, using premixed reagents
• Detect a single copy of a gene in as little as 10 ng of DNA
• Get reproducible, specific, and sensitive results
Easy to use
Pre-mixed, hot-start chemistries enable you to set up your experiments quickly. And by generating greater than 90% "A" overhangs—it makes cloning easier too.
Detect a single copy in 10 ng of DNA
Use the GeneAmp® Fast PCR Master Mix to detect a single copy of a gene in 10 ng of human genomic DNA or as little as 10 copies of an HIV target.
Part of the Applied Biosystems® Fast PCR System
The Applied Biosystems® Fast PCR System, consisting of the premixed GeneAmp® Fast PCR Master Mix, specially designed microplates and tubes, and the Veriti® 96-Well Thermal Cycler (0.1 mL or 0.2 mL), considerably reduces PCR reaction times.
PCR Master Mix (2X) (Thermo Scientific™)
Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimized for efficient and reproducible PCR.
Highlights
• Convenient, ready-to-use mix
• Thermostable—half life is more than 40 min at 95°C
• Generates PCR products with 3'-dA overhangs
• Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)
Applications
• Routine PCR amplification of DNA fragments up to 5 kb
• High throughput PCR
• DNA labeling
Note
• The error rate of Taq DNA Polymerase in PCR is 2.2 x 10-5 errors per nt per cycle, as determined by a modified method described in. Accordingly, the accuracy of PCR is 4.5 x 104. Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs.
Highlights
• Convenient, ready-to-use mix
• Thermostable—half life is more than 40 min at 95°C
• Generates PCR products with 3'-dA overhangs
• Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)
Applications
• Routine PCR amplification of DNA fragments up to 5 kb
• High throughput PCR
• DNA labeling
Note
• The error rate of Taq DNA Polymerase in PCR is 2.2 x 10-5 errors per nt per cycle, as determined by a modified method described in. Accordingly, the accuracy of PCR is 4.5 x 104. Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs.
AmpliTaq™ 360 DNA Polymerase (Applied Biosystems™)
The Applied Biosystems® AmpliTaq® 360 DNA Polymerase, when used with the new enhanced AmpliTaq 360 Buffer and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts. Compared to the original AmpliTaq® DNA polymerase, AmpliTaq® 360 polymerase is purified by an additional proprietary separation process which reduces contaminating bacterial DNA sequences from the enzyme preparation. This ultra-pure enzyme reduces false positive results, amplifies low-level target sequences, and when combined with the proprietary AmpliTaq® 360 Buffer Kit, promotes the amplification of a variety of templates including those from bacterial and human genomes.
* Optimized for the broadest range of targets—from everyday to challenging
* Unmatched sensitivity and yield
* Market-leading GC enhancer for robust amplification of GC-rich sequences
* Achieves the highest quality sequencing data
Extensively Tested
AmpliTaq® 360 DNA Polymerase has been extensively tested and optimized across a broad panel of difficult targets for best-in-class performance. Challenging targets include AT-rich, GC-rich, primer-dimer forming amplicons, homopolymer repeats, and amplicons that pose sequencing challenges. Amplicons that previously required specialized enzymes and reaction conditions can now be amplified reproducibly with a single reagent under standardized conditions.
Competitive benchmarking across more than 40 amplicons distinguishes AmpliTaq 360 as the best-performing enzyme, ensuring the highest probability of success for the amplification of both everyday and challenging targets (Figure 1). As shown in Figure 1, GC-rich regions are poorly amplified with other DNA polymerases while AmpliTaq 360 DNA Polymerase provides successful, robust amplification. These data are shown in a graphical format for specificity (Figure 2a) and specific yield (Figure 2b). The data averaged over all the targets is summarized in Table 1.
Superior Length and Sensitivity
AmpliTaq 360 DNA Polymerase reproducibly and efficiently amplifies long (up to 5 Kb) sequences. Figure 3 demonstrates high quality PCR amplification of long human and plasmid DNA. AmpliTaq 360 DNA Polymerase efficiently amplifies targets present at low copy number (Figure 4), even in the presence of high concentrations of complex DNA, making it especially suited for low-copy pathogen detection, multiplex PCR, allelic discrimination and amplification of targets from degraded DNA samples. The extreme purity of the enzyme, along with its validated and optimized buffer system, contributes to its unmatched sensitivity.
Superior Sensitivity, Reproducibility and Yield
Compared to the original AmpliTaq® DNA Polymerase, AmpliTaq® 360 DNA Polymerase is purified by an additional proprietary separation process which reduces contaminating bacterial DNA sequences from the enzyme preparation. When used with the enhanced 360 Buffer, this ultra-pure enzyme, reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates including those from bacterial and human genomes. This provides excellent specificity, reproducibility and yield across a broad range of targets (Figure 1).
Note: See user's manual or package insert for limited label license, and trademark information. For Research Use Only. Not for use in diagnostics procedures.
* Optimized for the broadest range of targets—from everyday to challenging
* Unmatched sensitivity and yield
* Market-leading GC enhancer for robust amplification of GC-rich sequences
* Achieves the highest quality sequencing data
Extensively Tested
AmpliTaq® 360 DNA Polymerase has been extensively tested and optimized across a broad panel of difficult targets for best-in-class performance. Challenging targets include AT-rich, GC-rich, primer-dimer forming amplicons, homopolymer repeats, and amplicons that pose sequencing challenges. Amplicons that previously required specialized enzymes and reaction conditions can now be amplified reproducibly with a single reagent under standardized conditions.
Competitive benchmarking across more than 40 amplicons distinguishes AmpliTaq 360 as the best-performing enzyme, ensuring the highest probability of success for the amplification of both everyday and challenging targets (Figure 1). As shown in Figure 1, GC-rich regions are poorly amplified with other DNA polymerases while AmpliTaq 360 DNA Polymerase provides successful, robust amplification. These data are shown in a graphical format for specificity (Figure 2a) and specific yield (Figure 2b). The data averaged over all the targets is summarized in Table 1.
Superior Length and Sensitivity
AmpliTaq 360 DNA Polymerase reproducibly and efficiently amplifies long (up to 5 Kb) sequences. Figure 3 demonstrates high quality PCR amplification of long human and plasmid DNA. AmpliTaq 360 DNA Polymerase efficiently amplifies targets present at low copy number (Figure 4), even in the presence of high concentrations of complex DNA, making it especially suited for low-copy pathogen detection, multiplex PCR, allelic discrimination and amplification of targets from degraded DNA samples. The extreme purity of the enzyme, along with its validated and optimized buffer system, contributes to its unmatched sensitivity.
Superior Sensitivity, Reproducibility and Yield
Compared to the original AmpliTaq® DNA Polymerase, AmpliTaq® 360 DNA Polymerase is purified by an additional proprietary separation process which reduces contaminating bacterial DNA sequences from the enzyme preparation. When used with the enhanced 360 Buffer, this ultra-pure enzyme, reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates including those from bacterial and human genomes. This provides excellent specificity, reproducibility and yield across a broad range of targets (Figure 1).
Note: See user's manual or package insert for limited label license, and trademark information. For Research Use Only. Not for use in diagnostics procedures.
AmpliTaq™ DNA Polymerase with Buffer II (25,000 units/tube) (Applied Biosystems™)
AmpliTaq® DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli. It is the most thoroughly characterized enzyme available for the PCR process and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial, lot-to-lot.
Features of this enzyme:
AmpliTaq® DNA Polymerase is the most thoroughly characterized enzyme available for PCR, a testimony to its overall utility and efficacy Its thermal activity profile makes it reliable for PCR applications It is QC-tested to guarantee reproducible results
Reliable and robust PCR
The thermal activity profile of AmpliTaq® DNA Polymerase is good for PCR applications because its optimal activity is in the same range at which stringent annealing of primers occurs (55–75°C). The enzyme's half-life is ~40 minutes at 95°C, providing thermostability that meets the requirements of most difficult PCR applications. This AmpliTaq® DNA Polymerase is supplied with GeneAmp® 10X PCR Buffer II. It is also available with GeneAmp® 10X PCR Buffer I and MgCl2 Solution.
Features of this enzyme:
Reliable and robust PCR
The thermal activity profile of AmpliTaq® DNA Polymerase is good for PCR applications because its optimal activity is in the same range at which stringent annealing of primers occurs (55–75°C). The enzyme's half-life is ~40 minutes at 95°C, providing thermostability that meets the requirements of most difficult PCR applications. This AmpliTaq® DNA Polymerase is supplied with GeneAmp® 10X PCR Buffer II. It is also available with GeneAmp® 10X PCR Buffer I and MgCl2 Solution.
Phusion High-Fidelity PCR Master Mix with GC Buffer (Thermo Scientific™)
Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase.
Phusion High-Fidelity PCR Master Mix is convenient 2X mix containing Phusion DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2. Two master mix formulations are available - with HF Buffer (F-531S and F-531L) and with GC Buffer (F-532S and F-532L). The error rate of Phusion DNA Polymerase in HF Buffer (4.4 × 10-7) is lower than that in GC Buffer (9.5 × 10-7). Therefore, the master mix with HF Buffer should be used as the default master mix for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures.
Highlights
• High fidelity (25X Taq)
• Fast PCR due to short extension times (15-30 s/kb)
• Robust performance, minimal optimization needed
Applications
• Amplification of difficult (GC-rich) templates
• High-fidelity PCR
• Cloning
• Template generation for sequencing
• Long-range PCR (up to 20 kb)
• Mutagenesis
• High throughput PCR
Phusion High-Fidelity PCR Master Mix is convenient 2X mix containing Phusion DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2. Two master mix formulations are available - with HF Buffer (F-531S and F-531L) and with GC Buffer (F-532S and F-532L). The error rate of Phusion DNA Polymerase in HF Buffer (4.4 × 10-7) is lower than that in GC Buffer (9.5 × 10-7). Therefore, the master mix with HF Buffer should be used as the default master mix for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures.
Highlights
• High fidelity (25X Taq)
• Fast PCR due to short extension times (15-30 s/kb)
• Robust performance, minimal optimization needed
Applications
• Amplification of difficult (GC-rich) templates
• High-fidelity PCR
• Cloning
• Template generation for sequencing
• Long-range PCR (up to 20 kb)
• Mutagenesis
• High throughput PCR
AmpliTaq Gold™ DNA Polymerase with Buffer I (Applied Biosystems™)
AmpliTaq Gold® DNA Polymerase is a chemically modified form of AmpliTaq® DNA Polymerase requiring thermal activation. Features of this enzyme:
• Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield
• Time-released thermal activation improves sensitivity in low copy number amplifications
• Successful multiplex PCR saves time and reagents
• Includes GeneAmp® 10X PCR Buffer I containing 15 mM MgCl2
Hot-start activation
The modified enzyme is provided in an inactive state. Upon thermal activation, the modifier is permanently released, regenerating active enzyme. The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional PCR techniques.
Higher specificity, higher yield
AmpliTaq Gold® DNA Polymerase can be activated partially or completely in a pre-PCR heat step, or can be allowed to activate slowly in a time-released manner during the denaturation steps of thermal cycling. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity. The yield of specific product increases because reactants are not wasted in the formation of unintended products. Because AmpliTaq Gold® DNA Polymerase is a chemical hot-start enzyme, there is no worry of biological contamination.
AmpliTaq Gold® 360 DNA Polymerase for superior performance
Also available is AmpliTaq Gold® 360 DNA Polymerase for even better PCR performance. Compared to AmpliTaq Gold® DNA Polymerase, AmpliTaq Gold® 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation. When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme, in addition to its hot-start capabilities, reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates. Also included with this product is a 360 GC Enhancer to work through challenging GC-rich templates.
• Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield
• Time-released thermal activation improves sensitivity in low copy number amplifications
• Successful multiplex PCR saves time and reagents
• Includes GeneAmp® 10X PCR Buffer I containing 15 mM MgCl2
Hot-start activation
The modified enzyme is provided in an inactive state. Upon thermal activation, the modifier is permanently released, regenerating active enzyme. The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional PCR techniques.
Higher specificity, higher yield
AmpliTaq Gold® DNA Polymerase can be activated partially or completely in a pre-PCR heat step, or can be allowed to activate slowly in a time-released manner during the denaturation steps of thermal cycling. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity. The yield of specific product increases because reactants are not wasted in the formation of unintended products. Because AmpliTaq Gold® DNA Polymerase is a chemical hot-start enzyme, there is no worry of biological contamination.
AmpliTaq Gold® 360 DNA Polymerase for superior performance
Also available is AmpliTaq Gold® 360 DNA Polymerase for even better PCR performance. Compared to AmpliTaq Gold® DNA Polymerase, AmpliTaq Gold® 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation. When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme, in addition to its hot-start capabilities, reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates. Also included with this product is a 360 GC Enhancer to work through challenging GC-rich templates.
Phusion Green Hot Start II High-Fidelity PCR Master Mix (Thermo Scientific™)
Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase.
Highlights
• Reaction set up at room temperature
• No non-specific amplification and primer degradation during reaction set up
• Zero-time reactivation due to unique hot start technology
• High fidelity (52X Taq)
• Fast PCR due to short extension times (15-30 s/kb)
• Robust reactions, minimal optimization needed
• Increased product yields with minimal enzyme amounts
• Direct loading on gels
With Phusion Hot Start II High-Fidelity DNA Polymerases amplification proceeds without the production of nonspecific products due to the combination of Phusion DNA Polymerase and a reversibly bound, specific Affibody ligand that inhibits DNA polymerase activity at room temperature. The Affibody ligand also inhibits the 3´→5´ exonuclease activity of the polymerase, thus preventing degradation of primers and template DNA during reaction set up. At temperatures that promote polymerase activity, the ligand is released, rendering the polymerase fully active. Phusion Hot Start II DNA Polymerase is immediately reactivated at high temperatures so it does not require a separate activation step in PCR protocols.
Phusion Green Hot Start II High-Fidelity PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. The master mix contains Phusion Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2. The buffer also includes a density reagent and two tracking dyes for direct loading of PCR products on a gel.
Applications
• High-fidelity PCR
• High throughput
• Amplification of difficult (GC-rich) templates
• Template generation for sequencing
• Multiplex PCR
• Long-range PCR
• Cloning
• Mutagenesis
• Microarray
Highlights
• Reaction set up at room temperature
• No non-specific amplification and primer degradation during reaction set up
• Zero-time reactivation due to unique hot start technology
• High fidelity (52X Taq)
• Fast PCR due to short extension times (15-30 s/kb)
• Robust reactions, minimal optimization needed
• Increased product yields with minimal enzyme amounts
• Direct loading on gels
With Phusion Hot Start II High-Fidelity DNA Polymerases amplification proceeds without the production of nonspecific products due to the combination of Phusion DNA Polymerase and a reversibly bound, specific Affibody ligand that inhibits DNA polymerase activity at room temperature. The Affibody ligand also inhibits the 3´→5´ exonuclease activity of the polymerase, thus preventing degradation of primers and template DNA during reaction set up. At temperatures that promote polymerase activity, the ligand is released, rendering the polymerase fully active. Phusion Hot Start II DNA Polymerase is immediately reactivated at high temperatures so it does not require a separate activation step in PCR protocols.
Phusion Green Hot Start II High-Fidelity PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. The master mix contains Phusion Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2. The buffer also includes a density reagent and two tracking dyes for direct loading of PCR products on a gel.
Applications
• High-fidelity PCR
• High throughput
• Amplification of difficult (GC-rich) templates
• Template generation for sequencing
• Multiplex PCR
• Long-range PCR
• Cloning
• Mutagenesis
• Microarray
