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EpiLife™ Medium, with 60 µM calcium (Gibco™)
Un milieu liquide, stérile préparé avec 60 µM de chlorure de calcium pour la culture à long terme, sans sérum de kératinocytes épidermiques humains et de cellules épithéliales cornéennes humaines. Ce milieu basal nécessite l’ajout d’un supplément de croissance approprié avant utilisation. EpiLife® prolonge amplement la durée de vie in vitro des kératinocytes épidermiques humains par rapport aux autres systèmes de milieux sans sérum. Ce milieu basal est exempt de produits d’origine animale et contient des acides aminés indispensables et non indispensables, des vitamines, d’autres composants organiques et des sels inorganiques. Il ne contient pas d’antibiotiques, d’antimycosiques, d’hormones, de facteurs de croissance ni de protéines. Le milieu EpiLife® est tamponné de HEPES et de bicarbonate, et il est conçu pour utilisation dans un incubateur avec une atmosphère de 5 % de CO2 et 95 % d’air.
EpiLife™ CF Kit (Gibco™)
A sterile, liquid medium prepared without calcium chloride for the long-term, serum-free culture of human epidermal keratinocytes and human corneal epithelial cells. This basal medium requires the addition of calcium plus an appropriate supplement prior to use. Calcium chloride is provided as a separate component with each bottle of medium. EpiLife greatly extends the in vitro lifespan of human epidermal keratinocytes compared to other serum-free media systems. This basal media is animal origin free and contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals, and inorganic salts. It does not contain antibiotics, antimycotics, hormones, growth factors, or proteins. EpiLife® medium is HEPES and bicarbonate buffered, and is designed for use in an incubator with an atmosphere of 5% CO2 and 95% air.
Keratinocyte AOF Growth Kit (Gibco™)
Growth kit for the animal origin-free culture of primary human keratinocytes. Contains 1 each of EpiLife Basal Medium (M-EPI-500-CA), Supplement S7 (S-017-5), and Coating Matrix Kit (R-011-K).
EpiLife™ CF/PRF Kit (Gibco™)
A sterile, liquid medium for the long-term, serum-free culture of human epidermal keratinocytes and human corneal epithelial cells. EpiLife® CF⁄PRF is prepared without calcium chloride and phenol red. Because this product is phenol red free, this product is ideal for oncology studies and when fluorescent microscopy is to be used. EpiLife® greatly extends the in vitro lifespan of human epidermal keratinocytes compared to other serum-free media systems. When used for cellular differentiation, the basal medium requires the addition of calcium plus an appropriate growth supplement prior to use. This basal media is animal origin free and contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals, and inorganic salts. It does not contain antibiotics, antimycotics, hormones, growth factors, or proteins. EpiLife® medium is HEPES and bicarbonate buffered, and is designed for use in an incubator with an atmosphere of 5% CO2 and 95% air.
