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VeriFiler™ Express PCR Amplification Kit (Applied Biosystems™)
The VeriFiler™ Express PCR Amplification Kit is a direct-amplification STR kit that combines a high power of discrimination with superior performance and fast time to results. The VeriFiler Express kit enables multiplex amplification of the 22 STR loci included in the GlobalFiler™ Express kit, as well the highly discriminating Penta D and Penta E loci and two gender markers (Amelogenin, Yindel) for maximum concordance with existing kits and high confidence in paternity and relationship testing conclusions.
Powered by breakthrough 6-dye technology, the VeriFiler Express PCR Amplification Kit was designed for optimal performance and excellent discrimination power to help obtain accurate results for single-source samples in less time.
Key features of the VeriFiler Express PCR Amplification Kit:
• Enables direct amplification from these common sample types used in paternity testing without the need for extraction or purification:
--Buccal samples on treated and untreated paper
--Buccal samples on cotton swab
--Blood samples on treated and untreated paper
• Helps accelerate time-to-result with minimal reduction in data quality
• Facilitates high throughput and automated processing through increased workflow efficiency
• Improves genotyping accuracy through superior primer design
• Enhances genotyping accuracy through expanded allelic ladder and virtual bins
High first-pass success rates improve productivity
The VeriFiler Express PCR Amplification Kit enables high-quality direct PCR amplification of reference samples with an improved first-pass success rate. You can expect:
• Well-resolved peaks above the background noise level
• Minimal occurrence of allelic drop-out, off-ladder allele calls, and off-scale data
• Well-balanced profile within each dye color for maximized Intracolor Balance (ICB) values
Maximizes concordance
The VeriFiler Express PCR Amplification primers have been optimized and tested thoroughly to maintain concordance with the GlobalFiler Express, NGM Select™, Identifiler™, and VeriFiler™ Direct kits thereby:
• Maximizing concordance with existing data sets for simplified historical comparisons
• Minimizing requirements for repeat analyses necessary to resolve non-concordant events
• Maintaining sufficient similarity of configuration for ease of training and implementation
Complete validated solution
Our products provide a complete solution for your human identification needs and include DNA quantification kits, a range of STR kits, instrumentation, software, and consumables. The VeriFiler Express PCR Amplification Kit is part of a comprehensively validated system for STR-based human identification. The PCR reaction components, primer sequences, and amplification protocols have been developed, optimized, and validated together with Applied Biosystems™ instrumentation and software to provide specific, robust amplification. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations such as the Scientific Working Group on DNA Analysis Methods (SWGDAM).
Validation experiments for the VeriFiler Express PCR Amplification Kit were performed by our scientists according to the Quality Assurance Standards for DNA Databasing Laboratories and the SWGDAM Validation Guidelines for DNA Analysis Methods. However, each laboratory using the VeriFiler Express PCR Amplification Kit should perform its own internal validation studies.
Commitment to quality
The VeriFiler Express PCR Amplification Kit is subjected to rigorous quality control testing to offer consistent performance. We develop and manufacture our products in accordance with ISO 9001 and ISO 18385 quality system requirements. Additionally, a Certificate of Analysis that confirms that the specific combination of components within each lot meets quality assurance testing specifications is available online.
Experience matters
We are the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical service scientists and field application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems human identification solutions in your laboratory.
For licensing and limited use restrictions visit www.thermofisher.com/HIDlicensing.
Powered by breakthrough 6-dye technology, the VeriFiler Express PCR Amplification Kit was designed for optimal performance and excellent discrimination power to help obtain accurate results for single-source samples in less time.
Key features of the VeriFiler Express PCR Amplification Kit:
• Enables direct amplification from these common sample types used in paternity testing without the need for extraction or purification:
--Buccal samples on treated and untreated paper
--Buccal samples on cotton swab
--Blood samples on treated and untreated paper
• Helps accelerate time-to-result with minimal reduction in data quality
• Facilitates high throughput and automated processing through increased workflow efficiency
• Improves genotyping accuracy through superior primer design
• Enhances genotyping accuracy through expanded allelic ladder and virtual bins
High first-pass success rates improve productivity
The VeriFiler Express PCR Amplification Kit enables high-quality direct PCR amplification of reference samples with an improved first-pass success rate. You can expect:
• Well-resolved peaks above the background noise level
• Minimal occurrence of allelic drop-out, off-ladder allele calls, and off-scale data
• Well-balanced profile within each dye color for maximized Intracolor Balance (ICB) values
Maximizes concordance
The VeriFiler Express PCR Amplification primers have been optimized and tested thoroughly to maintain concordance with the GlobalFiler Express, NGM Select™, Identifiler™, and VeriFiler™ Direct kits thereby:
• Maximizing concordance with existing data sets for simplified historical comparisons
• Minimizing requirements for repeat analyses necessary to resolve non-concordant events
• Maintaining sufficient similarity of configuration for ease of training and implementation
Complete validated solution
Our products provide a complete solution for your human identification needs and include DNA quantification kits, a range of STR kits, instrumentation, software, and consumables. The VeriFiler Express PCR Amplification Kit is part of a comprehensively validated system for STR-based human identification. The PCR reaction components, primer sequences, and amplification protocols have been developed, optimized, and validated together with Applied Biosystems™ instrumentation and software to provide specific, robust amplification. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations such as the Scientific Working Group on DNA Analysis Methods (SWGDAM).
Validation experiments for the VeriFiler Express PCR Amplification Kit were performed by our scientists according to the Quality Assurance Standards for DNA Databasing Laboratories and the SWGDAM Validation Guidelines for DNA Analysis Methods. However, each laboratory using the VeriFiler Express PCR Amplification Kit should perform its own internal validation studies.
Commitment to quality
The VeriFiler Express PCR Amplification Kit is subjected to rigorous quality control testing to offer consistent performance. We develop and manufacture our products in accordance with ISO 9001 and ISO 18385 quality system requirements. Additionally, a Certificate of Analysis that confirms that the specific combination of components within each lot meets quality assurance testing specifications is available online.
Experience matters
We are the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical service scientists and field application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems human identification solutions in your laboratory.
For licensing and limited use restrictions visit www.thermofisher.com/HIDlicensing.
AmpFLSTR™ Identifiler™ PCR Amplification Kit (Applied Biosystems™)
The AmpFLSTR® Identifiler® PCR Amplification Kit is the single most discriminating and widely used STR based kit for human identification applications from Applied Biosystems®. The Identifiler kit amplifies 15 loci and Amelogenin in a single tube and provides loci consistent with major world-wide STR databasing standards.
Features of the The AmpFLSTR® Identifiler® PCR Amplification Kit:
• Fifteen STR (short tandem repeat) loci and Amelogenin co-amplified in a single tube.
• Loci consistent with all major worldwide STR standards.
• Degenerate unlabeled primer addresses mutations observed in a population of Chamorros and Filipinos from Guam
• Includes 13 core loci required for sample entry in CODIS
Complete Validated Solution
Applied Biosystems offers a complete solution for your human identification needs. Our system includes DNA quantification kits, a range of AmpFLSTR® STR kits, instrumentation, software, and consumables. The Identifiler kit is part of the most comprehensively validated systems for STR-based Human Identification. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations such as SWGDAM and DAB.
Five Dye Technology Allows Enhanced Throughput
Identifiler utilizes the most advanced 5-dye technology to allow for the multiplex of more loci in a single lane or injection helping to increase your throughput and to simplify the workflow needed to obtain probative results. Our PET® and LIZ® -dyes were specifically designed to expand the spectral range of the 4-dye system. We have expanded the spectral range to 660 nm allowing for maximum color separation with minimal spectral overlap. As with all of our products, the five-dye technology is validated for use with our latest instruments and software as a complete system.
Experience Matters™
When you partner with us you gain access to the most extensive Human Identification resource network found within the industry. Our dedicated team of industry leading experts includes Research and Development scientists with extensive forensic product development experience, skilled Field Applications Support Scientists with over 100 years of combined forensic experience, and dedicated Field Service Engineers that provide on-site technical assistance with instrumentation.
For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.
Features of the The AmpFLSTR® Identifiler® PCR Amplification Kit:
• Fifteen STR (short tandem repeat) loci and Amelogenin co-amplified in a single tube.
• Loci consistent with all major worldwide STR standards.
• Degenerate unlabeled primer addresses mutations observed in a population of Chamorros and Filipinos from Guam
• Includes 13 core loci required for sample entry in CODIS
Complete Validated Solution
Applied Biosystems offers a complete solution for your human identification needs. Our system includes DNA quantification kits, a range of AmpFLSTR® STR kits, instrumentation, software, and consumables. The Identifiler kit is part of the most comprehensively validated systems for STR-based Human Identification. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations such as SWGDAM and DAB.
Five Dye Technology Allows Enhanced Throughput
Identifiler utilizes the most advanced 5-dye technology to allow for the multiplex of more loci in a single lane or injection helping to increase your throughput and to simplify the workflow needed to obtain probative results. Our PET® and LIZ® -dyes were specifically designed to expand the spectral range of the 4-dye system. We have expanded the spectral range to 660 nm allowing for maximum color separation with minimal spectral overlap. As with all of our products, the five-dye technology is validated for use with our latest instruments and software as a complete system.
Experience Matters™
When you partner with us you gain access to the most extensive Human Identification resource network found within the industry. Our dedicated team of industry leading experts includes Research and Development scientists with extensive forensic product development experience, skilled Field Applications Support Scientists with over 100 years of combined forensic experience, and dedicated Field Service Engineers that provide on-site technical assistance with instrumentation.
For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.
GlobalFiler™ Express PCR Amplification Kit (Applied Biosystems™)
The GlobalFiler® Express PCR Amplification Kit is the first 6-dye, 24-locus STR kit that combines maximum compatibility with global databasing loci standards, with dramatically reduced amplification time and superior discrimination power, to enable forensic DNA labs worldwide to maximize information recovery and improve overall efficiency. The GlobalFiler® Express kit is part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support. This kit is optimized for database and single-source samples; for casework samples, please see our GlobalFiler® PCR Amplification Kit.
Key Features:
• Up to 5-times faster amplification time than previous-generation kits
• DNA results in <2 hours—using current CE platforms
• Optimized protocols for blood and buccal samples deposited on swab or paper substrates
• The only kit with all markers recommended for inclusion by the CODIS Core Loci Working Group, including all markers commonly used in Europe, for maximum global database compatibility
The GlobalFiler® Express kit is a robust and reliable single-amplification STR multiplex kit optimized for use with database and single-source samples. The kit leverages new dyes for the incorporation of additional markers, generating the most discriminating kit available to the forensic community. The kit contains all of the markers included in the major global databases and utilizes optimized reagents, thermal cycling parameters, and the latest advances in oligo synthesis and purification to enable the highest quality results specific to the sample type being processed. Maintenance of the NGM SElect™ and Identifiler® primer sequences helps to ensure maximum concordance with previously typed samples. In addition, multiple gender markers have been added to the multiplex to enable accurate typing of male samples, even highly degraded or Y-negative samples, while increased genotyping accuracy is obtained with the expansion of the allelic ladder allele ranges to include all rare alleles as described in the literature (STRbase).
Experience Matters
We are the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical service scientists and field application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems® human identification solutions in your laboratory.
Key Features:
• Up to 5-times faster amplification time than previous-generation kits
• DNA results in <2 hours—using current CE platforms
• Optimized protocols for blood and buccal samples deposited on swab or paper substrates
• The only kit with all markers recommended for inclusion by the CODIS Core Loci Working Group, including all markers commonly used in Europe, for maximum global database compatibility
The GlobalFiler® Express kit is a robust and reliable single-amplification STR multiplex kit optimized for use with database and single-source samples. The kit leverages new dyes for the incorporation of additional markers, generating the most discriminating kit available to the forensic community. The kit contains all of the markers included in the major global databases and utilizes optimized reagents, thermal cycling parameters, and the latest advances in oligo synthesis and purification to enable the highest quality results specific to the sample type being processed. Maintenance of the NGM SElect™ and Identifiler® primer sequences helps to ensure maximum concordance with previously typed samples. In addition, multiple gender markers have been added to the multiplex to enable accurate typing of male samples, even highly degraded or Y-negative samples, while increased genotyping accuracy is obtained with the expansion of the allelic ladder allele ranges to include all rare alleles as described in the literature (STRbase).
Experience Matters
We are the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical service scientists and field application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems® human identification solutions in your laboratory.
AmpFLSTR™ Identifiler™ Plus PCR Amplification Kit (Applied Biosystems™)
Optimized for casework, the AmpFLSTR® Identifiler® Plus PCR Amplification Kit enables forensic analysts to recover more interpretable results from a wider range of DNA evidence samples with increased confidence. The new kit includes the same primers and allelic ladder as the widely used Identifiler® Kit and harnesses next-generation PCR amplification technology to help provide enhanced sensitivity, a cleaner baseline, improved performance on mixtures, and the ability to overcome high levels of PCR inhibition.
Features of the AmpFLSTR® Identifiler® Plus PCR Amplification Kit include:
• Amplifies the same loci as the Identifiler® kit with enhanced performance, data quality, and efficiency for casework samples
• Helps significantly improve performance on severely inhibited casework samples through the use of a high performance PCR buffer system and optimized thermal cycling parameters
• Yields higher peak heights from low-level samples and minor contributors in mixtures to facilitate interpretation
• Enables additional analytical flexibility by utilizing fully-validated 28- and 29-cycle protocols
• Streamlines workflow by enabling reduced amplification time
• Maintains the small amplicon size range of the Identifiler® kit (<360 bp) for recovery of information from degraded samples; works in conjunction with the MiniFiler™ Kit to help maximize recovery from heavily degraded samples
• Maintains primer sequences for genotyping concordance with samples typed with the Identifiler®, Identifiler® Direct, Profiler Plus®, COfiler® and SGM Plus® kits
• Utilizes an improved primer manufacturing process to help significantly reduce artifacts
Other Improvements and Benefits
The improvements in the Identifiler® Plus kit formulation allow for more rapid amplification, eliminating approximately 1 hour from the amplification step compared to the Identifiler® kit. In addition, the Identifiler® Plus Master Mix includes AmpliTaq Gold® DNA Polymerase, which eliminates 2 tubes and an extra box from the kit packaging, and enables streamlined reaction setup.
None of the formulation enhancements included in the Identifiler® Plus kit have required modifications to the Identifiler® Kit’s allelic ladder or 5-dye configuration. This configuration allows for optimal locus spacing and amplicon size ranges no greater than 360 bp, enabling better data recovery from degraded samples as compared to other multiplex systems with significantly larger amplicon size ranges. For heavily degraded samples, the MiniFiler™ kit has been designed to work in conjunction with the Identifiler® Plus kit, utilizing miniSTR technology to help maximize recovery of information.
The Identifiler® Plus kit also maintains primer sequences to enable genotyping concordance for samples previously typed using the Identifiler®, Identifiler® Direct, Profiler Plus®, COfiler® or SGM Plus® kits, and facilitate comparison with existing DNA databases.
The Confidence to Do More
The Identifiler® Plus kit is part of a new generation of AmpFLSTR® kits that harnesses significant advances in PCR amplification technology to enable recovery of more results from more samples with increased confidence.
Ultimately, laboratories using the Identifiler® Plus kit could significantly increase their ability to process difficult and challenging casework samples, and expand the types of cases from which probative results can be generated.
Experience Matters
We are the only company that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical Service scientists and Field Application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. Our team of forensic experts is also available to provide in-depth training and can support the planning and efficient execution of internal validation projects in your laboratory. All of these resources help ensure success when using our human identification solutions in your laboratory.
For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.
Features of the AmpFLSTR® Identifiler® Plus PCR Amplification Kit include:
• Amplifies the same loci as the Identifiler® kit with enhanced performance, data quality, and efficiency for casework samples
• Helps significantly improve performance on severely inhibited casework samples through the use of a high performance PCR buffer system and optimized thermal cycling parameters
• Yields higher peak heights from low-level samples and minor contributors in mixtures to facilitate interpretation
• Enables additional analytical flexibility by utilizing fully-validated 28- and 29-cycle protocols
• Streamlines workflow by enabling reduced amplification time
• Maintains the small amplicon size range of the Identifiler® kit (<360 bp) for recovery of information from degraded samples; works in conjunction with the MiniFiler™ Kit to help maximize recovery from heavily degraded samples
• Maintains primer sequences for genotyping concordance with samples typed with the Identifiler®, Identifiler® Direct, Profiler Plus®, COfiler® and SGM Plus® kits
• Utilizes an improved primer manufacturing process to help significantly reduce artifacts
Other Improvements and Benefits
The improvements in the Identifiler® Plus kit formulation allow for more rapid amplification, eliminating approximately 1 hour from the amplification step compared to the Identifiler® kit. In addition, the Identifiler® Plus Master Mix includes AmpliTaq Gold® DNA Polymerase, which eliminates 2 tubes and an extra box from the kit packaging, and enables streamlined reaction setup.
None of the formulation enhancements included in the Identifiler® Plus kit have required modifications to the Identifiler® Kit’s allelic ladder or 5-dye configuration. This configuration allows for optimal locus spacing and amplicon size ranges no greater than 360 bp, enabling better data recovery from degraded samples as compared to other multiplex systems with significantly larger amplicon size ranges. For heavily degraded samples, the MiniFiler™ kit has been designed to work in conjunction with the Identifiler® Plus kit, utilizing miniSTR technology to help maximize recovery of information.
The Identifiler® Plus kit also maintains primer sequences to enable genotyping concordance for samples previously typed using the Identifiler®, Identifiler® Direct, Profiler Plus®, COfiler® or SGM Plus® kits, and facilitate comparison with existing DNA databases.
The Confidence to Do More
The Identifiler® Plus kit is part of a new generation of AmpFLSTR® kits that harnesses significant advances in PCR amplification technology to enable recovery of more results from more samples with increased confidence.
Ultimately, laboratories using the Identifiler® Plus kit could significantly increase their ability to process difficult and challenging casework samples, and expand the types of cases from which probative results can be generated.
Experience Matters
We are the only company that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical Service scientists and Field Application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. Our team of forensic experts is also available to provide in-depth training and can support the planning and efficient execution of internal validation projects in your laboratory. All of these resources help ensure success when using our human identification solutions in your laboratory.
For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.
VeriFiler™ Express PCR Amplification Kit with Prep-n-Go™ Buffer (5 tubes) (Applied Biosystems™)
The VeriFiler™ Express PCR Amplification Kit with Prep-n-Go™ Buffer (5 tubes) combines the features of our VeriFiler™ Express PCR Amplification Kit with the convenience of sample processing using our Prep-n-Go™ Buffer. The VeriFiler Express kit is a direct-amplification STR kit that combines a high power of discrimination with superior performance and fast time to results. It enables multiplex amplification of the 22 STR loci included in the GlobalFiler™ Express kit as well the highly discriminating Penta D and Penta E loci and two gender markers (Amelogenin, Yindel) for maximum concordance with existing kits and high confidence in paternity and relationship testing conclusions.
Powered by breakthrough 6-dye technology, the VeriFiler Express PCR Amplification Kit was designed for optimal performance and excellent discrimination power to help obtain accurate results for single-source samples in less time.
Key features of the VeriFiler Express PCR Amplification Kit:
• Enables direct amplification from these common sample types used in paternity testing without the need for extraction or purification:
--Buccal samples on treated and untreated paper
--Buccal samples on cotton swab
--Blood samples on treated and untreated paper
• Helps accelerate time-to-result with minimal reduction in data quality
• Facilitates high throughput and automated processing through increased workflow efficiency
• Improves genotyping accuracy through superior primer design
• Enhances genotyping accuracy through expanded allelic ladder and virtual bins
High first-pass success rates improve productivity
The VeriFiler Express PCR Amplification Kit enables high-quality direct PCR amplification of reference samples with an improved first-pass success rate. You can expect:
• Well-resolved peaks above the background noise level
• Minimal occurrence of allelic drop-out, off-ladder allele calls, and off-scale data
• Well-balanced profile within each dye color for maximized Intracolor Balance (ICB) values
Maximizes concordance
The VeriFiler Express PCR Amplification primers have been optimized and tested thoroughly to maintain concordance with the GlobalFiler Express, NGM Select™, Identifiler™, and VeriFiler™ Direct kits thereby:
• Maximizing concordance with existing data sets for simplified historical comparisons
• Minimizing requirements for repeat analyses necessary to resolve non-concordant events
• Maintaining sufficient similarity of configuration for ease of training and implementation
Complete validated solution
Our products provide a complete solution for your human identification needs and include DNA quantification kits, a range of STR kits, instrumentation, software, and consumables. The VeriFiler Express PCR Amplification Kit is part of a comprehensively validated system for STR-based human identification. The PCR reaction components, primer sequences, and amplification protocols have been developed, optimized, and validated together with Applied Biosystems™ instrumentation and software to provide specific, robust amplification. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations such as the Scientific Working Group on DNA Analysis Methods (SWGDAM).
Validation experiments for the VeriFiler Express PCR Amplification Kit were performed by our scientists according to the Quality Assurance Standards for DNA Databasing Laboratories and the SWGDAM Validation Guidelines for DNA Analysis Methods. However, each laboratory using the VeriFiler Express PCR Amplification Kit should perform its own internal validation studies.
Commitment to quality
The VeriFiler Express PCR Amplification Kit is subjected to rigorous quality control testing to offer consistent performance. We develop and manufacture our products in accordance with ISO 9001 and ISO 18385 quality system requirements. Additionally, a Certificate of Analysis that confirms that the specific combination of components within each lot meets quality assurance testing specifications is available online.
Experience matters
We are the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical service scientists and field application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems human identification solutions in your laboratory.
For licensing and limited use restrictions visit www.thermofisher.com/HIDlicensing.
Powered by breakthrough 6-dye technology, the VeriFiler Express PCR Amplification Kit was designed for optimal performance and excellent discrimination power to help obtain accurate results for single-source samples in less time.
Key features of the VeriFiler Express PCR Amplification Kit:
• Enables direct amplification from these common sample types used in paternity testing without the need for extraction or purification:
--Buccal samples on treated and untreated paper
--Buccal samples on cotton swab
--Blood samples on treated and untreated paper
• Helps accelerate time-to-result with minimal reduction in data quality
• Facilitates high throughput and automated processing through increased workflow efficiency
• Improves genotyping accuracy through superior primer design
• Enhances genotyping accuracy through expanded allelic ladder and virtual bins
High first-pass success rates improve productivity
The VeriFiler Express PCR Amplification Kit enables high-quality direct PCR amplification of reference samples with an improved first-pass success rate. You can expect:
• Well-resolved peaks above the background noise level
• Minimal occurrence of allelic drop-out, off-ladder allele calls, and off-scale data
• Well-balanced profile within each dye color for maximized Intracolor Balance (ICB) values
Maximizes concordance
The VeriFiler Express PCR Amplification primers have been optimized and tested thoroughly to maintain concordance with the GlobalFiler Express, NGM Select™, Identifiler™, and VeriFiler™ Direct kits thereby:
• Maximizing concordance with existing data sets for simplified historical comparisons
• Minimizing requirements for repeat analyses necessary to resolve non-concordant events
• Maintaining sufficient similarity of configuration for ease of training and implementation
Complete validated solution
Our products provide a complete solution for your human identification needs and include DNA quantification kits, a range of STR kits, instrumentation, software, and consumables. The VeriFiler Express PCR Amplification Kit is part of a comprehensively validated system for STR-based human identification. The PCR reaction components, primer sequences, and amplification protocols have been developed, optimized, and validated together with Applied Biosystems™ instrumentation and software to provide specific, robust amplification. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations such as the Scientific Working Group on DNA Analysis Methods (SWGDAM).
Validation experiments for the VeriFiler Express PCR Amplification Kit were performed by our scientists according to the Quality Assurance Standards for DNA Databasing Laboratories and the SWGDAM Validation Guidelines for DNA Analysis Methods. However, each laboratory using the VeriFiler Express PCR Amplification Kit should perform its own internal validation studies.
Commitment to quality
The VeriFiler Express PCR Amplification Kit is subjected to rigorous quality control testing to offer consistent performance. We develop and manufacture our products in accordance with ISO 9001 and ISO 18385 quality system requirements. Additionally, a Certificate of Analysis that confirms that the specific combination of components within each lot meets quality assurance testing specifications is available online.
Experience matters
We are the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical service scientists and field application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems human identification solutions in your laboratory.
For licensing and limited use restrictions visit www.thermofisher.com/HIDlicensing.
AmpFLSTR™ Identifiler™ Direct PCR Amplification Kit (Applied Biosystems™)
The AmpFLSTR® Identifiler® Direct PCR Amplification Kit enables direct amplification of the 16 loci included in the Identifiler® Kit, eliminating the need to perform DNA extraction or purification steps for DNA database and other single source reference samples. Blood or buccal samples on FTA® Cards can be punched into PCR plates or tubes, and taken directly to PCR amplification, without reduction in data quality. Samples collected with the Buccal DNA Collector™ and other non-FTA® substrates can be processed without the need for heat incubation or additional workflow steps using the Applied Biosystems® Prep-n-Go™ Buffer.
Key Features of the AmpFLSTR® Identifiler® Direct PCR Amplification Kit:
• Enables the direct amplification of blood and buccal samples without the need for extraction or purification
• Facilitates high throughput & automated sample processing
• Minimizes the risk of contamination and procedural errors by reducing the number of processing steps
• Helps accelerate time to result with minimal reduction in data quality
• Minimizes extraction/purification costs and helps reduce the cost of labor and consumables
In addition, the Identifiler® Direct kit uses the same dye set, allelic ladder and primer sequences as the standard Identifiler® kit, which:
• Maximizes concordance with STR profiles generated with other AmpFLSTR® kits including the Identifiler®, Identifiler® Plus, Profiler Plus®, Profiler Plus® ID, SGM Plus® and COfiler® Kits
• Facilitates comparison with casework data and legacy databases
• Streamlines validation and implementation
Table 1: As part of its rigorous development, the Identifiler® Direct Kit was field tested by several medium to high-throughput DNA databasing laboratories to assess "first pass" success rate. Combined results from external test site laboratories are shown below. 1.2 mm punches from blood and buccal samples on FTA® Cards and Buccal DNA Collectors™ were amplified with the Identifiler® Direct Kit, and the resulting data was analyzed using GeneMapper® ID-X, at two different minimum peak amplitude thresholds.
The Confidence to Do More
The Identifiler® Direct Kit is part of a new generation of AmpFLSTR® Kits that harnesses significant advances in PCR amplification technology to enable recovery of more results from more samples with increased confidence.
Ultimately, laboratories using the Identifiler® Direct Kit will be able to significantly expand their throughput capabilities, while also maximizing sample integrity and quality assurance for the entire process.
Complete Validated Solution
We offer a complete solution to meet the needs of the human identification community. Our system includes DNA extraction and quantification kits, a range of AmpFLSTR® STR Kits, instrumentation, software, and consumables.
The Identifiler® Direct Kit is part of a comprehensively validated system for STR-based Human Identification. The PCR reaction components, primer sequences, and amplification protocols have been developed, optimized and validated together with Applied Biosystems® instrumentation and software to provide specific, robust amplification. Validation experiments for the Identifiler® Direct Kit were performed by our scientists according to the DNA Advisory Board (DAB) Quality Assurance Standards for Forensic DNA Testing Laboratories and the Scientific Working Group on DNA Analysis Methods (SWGDAM) Revised Validation Guidelines. However, each laboratory using the Identifiler® Direct Kit should perform its own internal validation studies.
The Identifiler® Direct Kit is subjected to rigorous quality control testing to ensure consistent performance. We develop and manufacture our products in accordance with ISO 9001 quality system requirements. Additionally, a Certificate of Analysis is available upon request that confirms that the specific combination of components within each lot meets quality assurance testing specifications.
Experience Matters
We are the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical Service Scientists and Field Application Specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems® Human Identification solutions in your laboratory.
Note: For Forensic or Paternity Use Only.
Important Notice
The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.
Key Features of the AmpFLSTR® Identifiler® Direct PCR Amplification Kit:
• Enables the direct amplification of blood and buccal samples without the need for extraction or purification
• Facilitates high throughput & automated sample processing
• Minimizes the risk of contamination and procedural errors by reducing the number of processing steps
• Helps accelerate time to result with minimal reduction in data quality
• Minimizes extraction/purification costs and helps reduce the cost of labor and consumables
In addition, the Identifiler® Direct kit uses the same dye set, allelic ladder and primer sequences as the standard Identifiler® kit, which:
• Maximizes concordance with STR profiles generated with other AmpFLSTR® kits including the Identifiler®, Identifiler® Plus, Profiler Plus®, Profiler Plus® ID, SGM Plus® and COfiler® Kits
• Facilitates comparison with casework data and legacy databases
• Streamlines validation and implementation
| Sample type | Success Rate (50 rfu Threshold) | Success Rate (150 rfu Threshold) | Number of Samples Tested |
| Blood on FTA™ Card | 99.4-100% Mean: 99.5% | 95.7-98.8% Mean: 97.3% | 414 |
| Buccal on FTA™ Card | 91.8-99.4% Mean: 97.1% | 84.2-95.5% Mean: 90.9% | 643 |
| Buccal on Bode Buccal DNA Collector™ Card | 97.5-98.8% Mean: 97.8% | 88.1-98.8% Mean: 94.0% | 582 |
Table 1: As part of its rigorous development, the Identifiler® Direct Kit was field tested by several medium to high-throughput DNA databasing laboratories to assess "first pass" success rate. Combined results from external test site laboratories are shown below. 1.2 mm punches from blood and buccal samples on FTA® Cards and Buccal DNA Collectors™ were amplified with the Identifiler® Direct Kit, and the resulting data was analyzed using GeneMapper® ID-X, at two different minimum peak amplitude thresholds.
The Confidence to Do More
The Identifiler® Direct Kit is part of a new generation of AmpFLSTR® Kits that harnesses significant advances in PCR amplification technology to enable recovery of more results from more samples with increased confidence.
Ultimately, laboratories using the Identifiler® Direct Kit will be able to significantly expand their throughput capabilities, while also maximizing sample integrity and quality assurance for the entire process.
Complete Validated Solution
We offer a complete solution to meet the needs of the human identification community. Our system includes DNA extraction and quantification kits, a range of AmpFLSTR® STR Kits, instrumentation, software, and consumables.
The Identifiler® Direct Kit is part of a comprehensively validated system for STR-based Human Identification. The PCR reaction components, primer sequences, and amplification protocols have been developed, optimized and validated together with Applied Biosystems® instrumentation and software to provide specific, robust amplification. Validation experiments for the Identifiler® Direct Kit were performed by our scientists according to the DNA Advisory Board (DAB) Quality Assurance Standards for Forensic DNA Testing Laboratories and the Scientific Working Group on DNA Analysis Methods (SWGDAM) Revised Validation Guidelines. However, each laboratory using the Identifiler® Direct Kit should perform its own internal validation studies.
The Identifiler® Direct Kit is subjected to rigorous quality control testing to ensure consistent performance. We develop and manufacture our products in accordance with ISO 9001 quality system requirements. Additionally, a Certificate of Analysis is available upon request that confirms that the specific combination of components within each lot meets quality assurance testing specifications.
Experience Matters
We are the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical Service Scientists and Field Application Specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems® Human Identification solutions in your laboratory.
Note: For Forensic or Paternity Use Only.
Important Notice
The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.
Yfiler™ Plus PCR Amplification Kit (Applied Biosystems™)
Note: The Yfiler Plus kit is now approved for use by laboratories generating DNA profiles for inclusion in the US National DNA Index System (NDIS) CODIS database.
The Yfiler® Plus PCR Amplification Kit is designed for forensic laboratories that process highly challenging Y-STR casework and database samples. The Yfiler® Plus kit is optimized to help improve resolution of trace evidence and sexual assault mixtures that contain small quantities of male DNA in a large background of female DNA and offers laboratories both the power of discrimination and the performance to enable accurate results in less time from challenging samples.
The Yfiler® Plus PCR Amplification Kit is a 6-dye multiplex assay for short tandem repeats (STRs) that allows amplification from multiple male-specific sample types, such as male–male and male–female mixtures, and direct PCR amplification from single-source samples.
Key features of the Yfiler® Plus PCR Amplification Kit:
• Improved discrimination capacity through new, highly discriminating markers
• Improved performance with degraded samples and PCR inhibitors
• Improved genotyping accuracy through expanded allelic ladder and virtual bins
• Concordance with existing data in reference haplotype databases
• Increased workflow efficiency enabling faster time-to-results than previous Y-STR kits
Improved discrimination capacity
The Yfiler® Plus PCR Amplification Kit enables multiplex amplification of the 17 Y-STR markers included in the AmpFLSTR® Yfiler® PCR Amplification Kits, as well as 10 new Y-STRs using 6-dye chemistry to offer the highest discriminatory capacity of any commercially available Y-STR kit. In particular, the inclusion of seven rapidly-mutating (RM) Y-STRs with mutation rates above 1 × 10(-2) helps improve resolution of paternal lineage differentiation as well as helps with discrimination of closely-related males.
More information from challenging casework samples
Y-STR analysis is useful for processing of complex casework samples with trace quantities of male DNA in presence of high level of female DNA. These samples may include rape kit samples, body fluid with few or no sperm present, and fingernail scrapings from female victims of violent assaults. These samples often show DNA degradation and contain inhibitors that can make the analysis difficult and lead to inconclusive results. The Yfiler® Plus kit harnesses the latest developments in PCR amplification technology to help provide cleaner baseline and improved recovery of alleles with mixtures and highly inhibited samples.
Streamlined protocol and increased workflow efficiency
The Yfiler® Plus kit provides faster thermal cycling conditions enabling shorter time to result. The kit is also designed to process single-source reference samples using direct PCR amplification from the following types of samples:
• Blood and buccal samples on treated paper substrates, without the need for sample purification
• Blood samples collected on untreated paper substrates and treated with Prep-n-Go™ Buffer
• Buccal samples collected on swab substrates and treated with Prep-n-Go™ Buffer
Complete validated solution
Our products provide a complete solution for your human identification needs and include DNA quantification kits, a range of STR kits, instrumentation, software, and consumables. The Yfiler® Plus kit is part of a comprehensively validated system for STR-based human identification. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations such as SWGDAM and DAB.
For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.
The Yfiler® Plus PCR Amplification Kit is designed for forensic laboratories that process highly challenging Y-STR casework and database samples. The Yfiler® Plus kit is optimized to help improve resolution of trace evidence and sexual assault mixtures that contain small quantities of male DNA in a large background of female DNA and offers laboratories both the power of discrimination and the performance to enable accurate results in less time from challenging samples.
The Yfiler® Plus PCR Amplification Kit is a 6-dye multiplex assay for short tandem repeats (STRs) that allows amplification from multiple male-specific sample types, such as male–male and male–female mixtures, and direct PCR amplification from single-source samples.
Key features of the Yfiler® Plus PCR Amplification Kit:
• Improved discrimination capacity through new, highly discriminating markers
• Improved performance with degraded samples and PCR inhibitors
• Improved genotyping accuracy through expanded allelic ladder and virtual bins
• Concordance with existing data in reference haplotype databases
• Increased workflow efficiency enabling faster time-to-results than previous Y-STR kits
Improved discrimination capacity
The Yfiler® Plus PCR Amplification Kit enables multiplex amplification of the 17 Y-STR markers included in the AmpFLSTR® Yfiler® PCR Amplification Kits, as well as 10 new Y-STRs using 6-dye chemistry to offer the highest discriminatory capacity of any commercially available Y-STR kit. In particular, the inclusion of seven rapidly-mutating (RM) Y-STRs with mutation rates above 1 × 10(-2) helps improve resolution of paternal lineage differentiation as well as helps with discrimination of closely-related males.
More information from challenging casework samples
Y-STR analysis is useful for processing of complex casework samples with trace quantities of male DNA in presence of high level of female DNA. These samples may include rape kit samples, body fluid with few or no sperm present, and fingernail scrapings from female victims of violent assaults. These samples often show DNA degradation and contain inhibitors that can make the analysis difficult and lead to inconclusive results. The Yfiler® Plus kit harnesses the latest developments in PCR amplification technology to help provide cleaner baseline and improved recovery of alleles with mixtures and highly inhibited samples.
Streamlined protocol and increased workflow efficiency
The Yfiler® Plus kit provides faster thermal cycling conditions enabling shorter time to result. The kit is also designed to process single-source reference samples using direct PCR amplification from the following types of samples:
• Blood and buccal samples on treated paper substrates, without the need for sample purification
• Blood samples collected on untreated paper substrates and treated with Prep-n-Go™ Buffer
• Buccal samples collected on swab substrates and treated with Prep-n-Go™ Buffer
Complete validated solution
Our products provide a complete solution for your human identification needs and include DNA quantification kits, a range of STR kits, instrumentation, software, and consumables. The Yfiler® Plus kit is part of a comprehensively validated system for STR-based human identification. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations such as SWGDAM and DAB.
For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.
AmpFLSTR™ Huaxia™ PCR Direct Amplification Kit (Applied Biosystems™)
The AmpFLSTR® Huaxia™ PCR Direct Amplification Kit employs a validated "direct" amplification chemistry designed specifically for the China forensic community to use with DNA database samples and other single source DNA samples. The Huaxia™ PCR Direct Amplification kit amplifies the discriminating loci set in the Applied Biosystems® Sinofiler™ Kit, while enabling high quality direct PCR amplification of reference samples without the need for incubation, extraction, or purification.
The Huaxia™ PCR Direct Amplification kit:
• Facilitates high throughput and automated processing
• Accelerates time-to-result while maintaining high profile quality
• Eliminates extraction/purification costs and reduces labor and consumable costs
• Reduces the number of pre-amplification processing steps, minimizing the possibility of contamination or other procedural errors
• Uses the same loci and primer sequences as the Sinofiler™ kit, which:
-- Offers high discrimination for Chinese populations
--Maximizes concordance and facilitates comparison with existing data/database
--Streamlines validation and implementation
Direct Amplification While Minimizing PCR Inhibition
By overcoming PCR inhibition and enhancing amplification of all the Sinofiler™ loci, the Huaxia™ PCR Direct Amplification kit delivers direct amplification capability to the Sinofiler™ primer set, allowing database laboratories to eliminate work steps and reduce time and labor required to generate STR profiles. In addition, the Huaxia™ kit allows for amplification in less time than is currently required.
Higher First-pass Success Rates Improve Productivity
The Huaxia™ PCR Direct Amplification kit enables high-quality direct PCR amplification of reference samples with an improved first-pass success rate. You can expect:
• Well-resolved peaks above the background 'noise' level
• Minimal occurrence of allelic drop-out or off-scale data
• Clean baselines with fewer artifacts
• Well-balanced profile within each dye color for maximized Intracolor Balance (ICB) values
With the Huaxia™ kit, there is no need for sample extraction, purification, or quantification, enabling a substantial reduction in labor and consumable costs compared to the standard PCR amplification workflow.
Simply "Punch and Go"
The kit includes primer mix, reaction mix, positive control DNA, and allelic ladder. The enzyme is contained within the reaction mix, so the Huaxia™ kit contains fewer tubes and fewer boxes than the Sinofiler™ kit. Simply punch a 1.2 mm disc into each well of a 96-well plate, add the master mix, and go to thermocycling. No washing, no extracting, and no quantifying. It's as simple as punch and go.
Note: For Forensic or Paternity Use Only.
IMPORTANT NOTICE
The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.
The Huaxia™ PCR Direct Amplification kit:
• Facilitates high throughput and automated processing
• Accelerates time-to-result while maintaining high profile quality
• Eliminates extraction/purification costs and reduces labor and consumable costs
• Reduces the number of pre-amplification processing steps, minimizing the possibility of contamination or other procedural errors
• Uses the same loci and primer sequences as the Sinofiler™ kit, which:
-- Offers high discrimination for Chinese populations
--Maximizes concordance and facilitates comparison with existing data/database
--Streamlines validation and implementation
Direct Amplification While Minimizing PCR Inhibition
By overcoming PCR inhibition and enhancing amplification of all the Sinofiler™ loci, the Huaxia™ PCR Direct Amplification kit delivers direct amplification capability to the Sinofiler™ primer set, allowing database laboratories to eliminate work steps and reduce time and labor required to generate STR profiles. In addition, the Huaxia™ kit allows for amplification in less time than is currently required.
Higher First-pass Success Rates Improve Productivity
The Huaxia™ PCR Direct Amplification kit enables high-quality direct PCR amplification of reference samples with an improved first-pass success rate. You can expect:
• Well-resolved peaks above the background 'noise' level
• Minimal occurrence of allelic drop-out or off-scale data
• Clean baselines with fewer artifacts
• Well-balanced profile within each dye color for maximized Intracolor Balance (ICB) values
With the Huaxia™ kit, there is no need for sample extraction, purification, or quantification, enabling a substantial reduction in labor and consumable costs compared to the standard PCR amplification workflow.
Simply "Punch and Go"
The kit includes primer mix, reaction mix, positive control DNA, and allelic ladder. The enzyme is contained within the reaction mix, so the Huaxia™ kit contains fewer tubes and fewer boxes than the Sinofiler™ kit. Simply punch a 1.2 mm disc into each well of a 96-well plate, add the master mix, and go to thermocycling. No washing, no extracting, and no quantifying. It's as simple as punch and go.
Note: For Forensic or Paternity Use Only.
IMPORTANT NOTICE
The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.
GlobalFiler™ PCR Amplification Kit (Applied Biosystems™)
The GlobalFiler® PCR Amplification Kit is the first 6-dye, 24-locus STR kit that combines maximum compatibility with global databasing loci standards, with dramatically reduced amplification time and superior discrimination power, helping to enable forensic DNA labs worldwide to maximize information recovery and improve overall efficiency. The GlobalFiler® kit is part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support. This kit is optimized for casework samples; for database and single-source samples, please see our GlobalFiler® Express PCR Amplification Kit.
Key Features:
• Efficient protocols minimize amplification time compared to previous-generation kits
• An optimized buffer system and expanded DNA input volume delivers maximum sensitivity for trace/low level DNA samples
• Enhanced intracolor balance simplifies interpretation of mixture samples
• The inclusion of 10 mini-STRs maximizes results from degraded samples
• The only kit with all markers recommended for inclusion by the CODIS Core Loci Working Group, including all markers commonly used in Europe, for maximum global database compatibility
The GlobalFiler® kit is a robust and reliable single-amplification STR multiplex kit optimized for use with the most challenging casework samples. The kit leverages new dyes for the incorporation of additional markers, generating the most discriminating kit available to the forensic community. The kit contains all of the markers included in the major global databases, including 10 mini-STRs, designed to deliver optimized results with even highly degraded samples.
Developed specifically for casework applications, the GlobalFiler® kit contains a buffer system that delivers greater sensitivity for enhanced performance with inhibited and low-concentration DNA samples. The GlobalFiler® kit utilizes the latest advances in oligo synthesis and purification, which provide a clean baseline and a balanced profile, resulting in simplified data analysis. Maintenance of the NGM SElect™ and Identifiler® primer sequences helps ensure maximum concordance with previously typed samples. In addition, multiple gender markers have been added to the multiplex to enable accurate typing of male samples, even highly degraded or Y-negative samples, while increased genotyping accuracy is obtained with the expansion of the allelic ladder allele ranges to include all rare alleles as described in the literature (STRbase).
Experience Matters
We are the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical service scientists and field application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems® human identification solutions in your laboratory.
For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.
Key Features:
• Efficient protocols minimize amplification time compared to previous-generation kits
• An optimized buffer system and expanded DNA input volume delivers maximum sensitivity for trace/low level DNA samples
• Enhanced intracolor balance simplifies interpretation of mixture samples
• The inclusion of 10 mini-STRs maximizes results from degraded samples
• The only kit with all markers recommended for inclusion by the CODIS Core Loci Working Group, including all markers commonly used in Europe, for maximum global database compatibility
The GlobalFiler® kit is a robust and reliable single-amplification STR multiplex kit optimized for use with the most challenging casework samples. The kit leverages new dyes for the incorporation of additional markers, generating the most discriminating kit available to the forensic community. The kit contains all of the markers included in the major global databases, including 10 mini-STRs, designed to deliver optimized results with even highly degraded samples.
Developed specifically for casework applications, the GlobalFiler® kit contains a buffer system that delivers greater sensitivity for enhanced performance with inhibited and low-concentration DNA samples. The GlobalFiler® kit utilizes the latest advances in oligo synthesis and purification, which provide a clean baseline and a balanced profile, resulting in simplified data analysis. Maintenance of the NGM SElect™ and Identifiler® primer sequences helps ensure maximum concordance with previously typed samples. In addition, multiple gender markers have been added to the multiplex to enable accurate typing of male samples, even highly degraded or Y-negative samples, while increased genotyping accuracy is obtained with the expansion of the allelic ladder allele ranges to include all rare alleles as described in the literature (STRbase).
Experience Matters
We are the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical service scientists and field application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems® human identification solutions in your laboratory.
For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.
AmpFLSTR™ Yfiler™ Direct Kit (Applied Biosystems™)
The AmpFLSTR® Yfiler® Direct Kit enables forensic laboratories to perform high-quality Y-STR analysis of single-source reference samples using direct PCR amplification. This kit amplifies the same 17 Y-STR loci as the AmpFLSTR® Yfiler® Kit and is designed to generate accurate results with faster thermocycling conditions than the Yfiler® Kit. Blood or buccal samples on treated paper (FTA® Card or NUCLEIC-CARD™ System) can be punched into PCR plates or tubes and taken directly to PCR amplification, without reduction in data quality. Samples collected with the Buccal DNA Collector™ and other untreated substrates can be processed without additional workflow steps using the Applied Biosystems® Prep-n-Go™ Buffer.
Key Features:
• Supports direct PCR amplification (no DNA extraction or quantification required) of single source reference samples on treated and untreated paper
• Provides maximum concordance with the Yfiler® kit
• Designed to deliver faster cycling times through the introduction of a new fast-capable enzyme (~2.5 hours)
In addition, the Yfiler® Direct kit uses the same dye set, allelic ladder, and primer sequences as the standard Yfiler® kit, which:
• Facilitates comparison with casework data
• Streamlines validation and implementation
Complete Validated Solution
We offer a complete solution to meet the needs of the human identification community. Our system includes DNA extraction and quantification kits, a range of AmpFLSTR® STR Kits, instrumentation, software, and consumables.
The AmpFLSTR® Yfiler® Direct Kit is part of a comprehensively validated system for STR-based human identification. The PCR reaction components, primer sequences, and amplification protocols have been developed, optimized, and validated together with Applied Biosystems® instrumentation and software to provide specific, robust amplification.
The Yfiler® Direct kit is subjected to rigorous quality control testing to ensure consistent performance. We develop and manufacture our products in accordance with ISO 9001 quality system requirements. Additionally, a Certificate of Analysis is available upon request that confirms that the specific combination of components within each lot meets quality assurance testing specifications.
Experience Matters
We are the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical Service Scientists and Field Application Specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems® human identification solutions in your laboratory.
IMPORTANT NOTICE:
The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.
For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.
Key Features:
• Supports direct PCR amplification (no DNA extraction or quantification required) of single source reference samples on treated and untreated paper
• Provides maximum concordance with the Yfiler® kit
• Designed to deliver faster cycling times through the introduction of a new fast-capable enzyme (~2.5 hours)
In addition, the Yfiler® Direct kit uses the same dye set, allelic ladder, and primer sequences as the standard Yfiler® kit, which:
• Facilitates comparison with casework data
• Streamlines validation and implementation
Complete Validated Solution
We offer a complete solution to meet the needs of the human identification community. Our system includes DNA extraction and quantification kits, a range of AmpFLSTR® STR Kits, instrumentation, software, and consumables.
The AmpFLSTR® Yfiler® Direct Kit is part of a comprehensively validated system for STR-based human identification. The PCR reaction components, primer sequences, and amplification protocols have been developed, optimized, and validated together with Applied Biosystems® instrumentation and software to provide specific, robust amplification.
The Yfiler® Direct kit is subjected to rigorous quality control testing to ensure consistent performance. We develop and manufacture our products in accordance with ISO 9001 quality system requirements. Additionally, a Certificate of Analysis is available upon request that confirms that the specific combination of components within each lot meets quality assurance testing specifications.
Experience Matters
We are the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical Service Scientists and Field Application Specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems® human identification solutions in your laboratory.
IMPORTANT NOTICE:
The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.
For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.
AmpFLSTR™ SGM Plus™ PCR Amplification Kit (Applied Biosystems™)
The AmpFLSTR® SGM Plus® PCR Amplification Kit amplifies 10 STR loci and the gender marker Amelogenin yielding a highly informative kit for human identification, with a 1 in 5 X 1012 average probability of identity.
Key Features of the AmpFLSTR® SGM Plus® PCR Amplification Kit:
• Ten tetranucleotide STR loci and Amelogenin are co-amplified in a single PCR tube for ease of use and increased throughput
• Amplicon allele size ranges less than 360 base pairs ensure robust PCR amplification for all loci
• Conveys certain rights to perform research and forensic testing services when used with an authorized thermal cycler
• Conveys the PCR service rights for paternity testing
More Information
Six of the STR loci (D21, FGA, TH01, vWA, D8, D18) were developed and popularized by the Forensic Science Service (FSS) in the Second Generation Multiplex (SGM) system. To create the AmpFLSTR® SGM Plus® kit, a PCR multiplex was developed and optimized to included the six core SGM loci and Amelogenin, plus four additional STR loci (D3, D19, D16, D2). The AmpFLSTR® SGM Plus® kit significantly enhances the information obtained from a single PCR.
Reliable Results
The AmpFLSTR® SGM Plus® kit has been subjected to rigorous quality-control testing to ensure reliable performance. Experiments have been performed based upon the TWGDAM recommended guidelines (Technical Working Group on DNA Analysis Methods. 1995. Guidelines for a Quality Assurance Program for DNA Analysis. Crime Lab Digest 22:21 to 43).
Note: For Forensic or Paternity Use Only.
Important Notice
The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.
Key Features of the AmpFLSTR® SGM Plus® PCR Amplification Kit:
• Ten tetranucleotide STR loci and Amelogenin are co-amplified in a single PCR tube for ease of use and increased throughput
• Amplicon allele size ranges less than 360 base pairs ensure robust PCR amplification for all loci
• Conveys certain rights to perform research and forensic testing services when used with an authorized thermal cycler
• Conveys the PCR service rights for paternity testing
More Information
Six of the STR loci (D21, FGA, TH01, vWA, D8, D18) were developed and popularized by the Forensic Science Service (FSS) in the Second Generation Multiplex (SGM) system. To create the AmpFLSTR® SGM Plus® kit, a PCR multiplex was developed and optimized to included the six core SGM loci and Amelogenin, plus four additional STR loci (D3, D19, D16, D2). The AmpFLSTR® SGM Plus® kit significantly enhances the information obtained from a single PCR.
Reliable Results
The AmpFLSTR® SGM Plus® kit has been subjected to rigorous quality-control testing to ensure reliable performance. Experiments have been performed based upon the TWGDAM recommended guidelines (Technical Working Group on DNA Analysis Methods. 1995. Guidelines for a Quality Assurance Program for DNA Analysis. Crime Lab Digest 22:21 to 43).
Note: For Forensic or Paternity Use Only.
Important Notice
The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.
AmpFLSTR™ NGM SElect™ Express Kit (Applied Biosystems™)
The AmpFℓSTR® NGM SElect™ Express Kit enables extremely efficient analysis of single-source samples for laboratories wishing to utilize the European Standard Set Loci (ESSL) together with the highly discriminating SE33 locus. The kit utilizes the same primer sequences as the NGM™ and NGM SElect™ kits, but employs a new master mix optimized to enable high quality direct amplification of swab and treated/untreated paper substrates, as well as rapid PCR cycling (< 1 hr).
Key Features of the AmpFℓSTR® NGM SElect™ Express Kit:
• Supports rapid, direct amplification on treated paper and untreated paper/swab substrates in conjunction with Prep-n-Go™ Buffer
- Facilitates high throughput & automated processing
- Minimizes extraction, purification, and quantification costs and helps reduce labor and consumable costs
• Contains a new fast-capable enzyme that allows completion of amplification in ~45 mins
• Maximizes first pass success rate, reducing the need for time-consuming repeat amplifications
• Part of our complete integrated solution for efficient single-source sample processing
• Enables laboratories to go from sample to result typically in only 2 hours!
Data Quality Worth Sharing
The AmpFℓSTR® NGM SElect™ Express Kit has been designed to deliver a powerful level of discrimination and data quality to support cross-border data sharing initiatives. The kit addresses the requirements stipulated by the European Network of Forensic Science Institutes (ENFSI) and European DNA Profiling Group (EDNAP) and includes the expanded European Standard Set of Loci (ESSL). The NGM SElect™ Express kit simultaneously amplifies the same 16 loci included in the NGM™ kit (D3S1358, vWA, D16S539, D2S1338, D8S1179, D21S11, D18S51, D19S433, TH01, FGA, Amelogenin, D10S1248, D22S1045, D2S441, D1S1656, D12S391) with the addition of the highly polymorphic SE33 locus in the PET (red) dye channel.
Powerful Discrimination
The combination of loci in the NGM SElect™ Express kit generates an extremely high power of discrimination of any AmpFℓSTR® kit (see Table 1).
Maximizes Concordance
The NGM SElect™ Express kit maintains identical primer sequences for the core STR loci common to the SGM Plus®, Identifiler® and SEfiler Plus™ kits, thereby:
• Maximizing concordance with existing data sets for simplified historical comparisons
• Minimizing requirements for repeat analyses necessary to resolve non-concordant events
• Maintaining sufficient similarity of configuration with previous kits to reduce training and implementation requirements
The primers for the SE33 locus were redesigned from those used in the SEfiler Plus™ kit to relocate the amplicon within the multiplex. However, the new primer sequences have been optimized and tested thoroughly to maintain concordance with the SEfiler Plus™ kit.
Easy to Implement, Easy to Use
Every aspect of the NGM SElect™ Express kit has been designed with ease of implementation and use in mind. This is a single kit solution that is designed to deliver high quality results for both database and casework samples, optimizing the forensic DNA workflow while reducing operating costs. The kit workflow is very similar to other AmpFℓSTR® kits, enabling forensic laboratories to streamline validation and implementation. However, the enzyme is now contained within the master mix, simplifying reaction set-up. Cycling times have been shortened to less than one hour, allowing laboratories to achieve higher quality results more quickly. For compatibility with both manual and automated systems, two package sizes are available, the standard 200 reaction kit and a larger 1000 reaction kit. The larger package includes larger bottles to facilitate use on liquid handling platforms.
For Forensic or Paternity Use Only.
Important Notice
The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.
Key Features of the AmpFℓSTR® NGM SElect™ Express Kit:
• Supports rapid, direct amplification on treated paper and untreated paper/swab substrates in conjunction with Prep-n-Go™ Buffer
- Facilitates high throughput & automated processing
- Minimizes extraction, purification, and quantification costs and helps reduce labor and consumable costs
• Contains a new fast-capable enzyme that allows completion of amplification in ~45 mins
• Maximizes first pass success rate, reducing the need for time-consuming repeat amplifications
• Part of our complete integrated solution for efficient single-source sample processing
• Enables laboratories to go from sample to result typically in only 2 hours!
Data Quality Worth Sharing
The AmpFℓSTR® NGM SElect™ Express Kit has been designed to deliver a powerful level of discrimination and data quality to support cross-border data sharing initiatives. The kit addresses the requirements stipulated by the European Network of Forensic Science Institutes (ENFSI) and European DNA Profiling Group (EDNAP) and includes the expanded European Standard Set of Loci (ESSL). The NGM SElect™ Express kit simultaneously amplifies the same 16 loci included in the NGM™ kit (D3S1358, vWA, D16S539, D2S1338, D8S1179, D21S11, D18S51, D19S433, TH01, FGA, Amelogenin, D10S1248, D22S1045, D2S441, D1S1656, D12S391) with the addition of the highly polymorphic SE33 locus in the PET (red) dye channel.
Powerful Discrimination
The combination of loci in the NGM SElect™ Express kit generates an extremely high power of discrimination of any AmpFℓSTR® kit (see Table 1).
Maximizes Concordance
The NGM SElect™ Express kit maintains identical primer sequences for the core STR loci common to the SGM Plus®, Identifiler® and SEfiler Plus™ kits, thereby:
• Maximizing concordance with existing data sets for simplified historical comparisons
• Minimizing requirements for repeat analyses necessary to resolve non-concordant events
• Maintaining sufficient similarity of configuration with previous kits to reduce training and implementation requirements
The primers for the SE33 locus were redesigned from those used in the SEfiler Plus™ kit to relocate the amplicon within the multiplex. However, the new primer sequences have been optimized and tested thoroughly to maintain concordance with the SEfiler Plus™ kit.
Easy to Implement, Easy to Use
Every aspect of the NGM SElect™ Express kit has been designed with ease of implementation and use in mind. This is a single kit solution that is designed to deliver high quality results for both database and casework samples, optimizing the forensic DNA workflow while reducing operating costs. The kit workflow is very similar to other AmpFℓSTR® kits, enabling forensic laboratories to streamline validation and implementation. However, the enzyme is now contained within the master mix, simplifying reaction set-up. Cycling times have been shortened to less than one hour, allowing laboratories to achieve higher quality results more quickly. For compatibility with both manual and automated systems, two package sizes are available, the standard 200 reaction kit and a larger 1000 reaction kit. The larger package includes larger bottles to facilitate use on liquid handling platforms.
For Forensic or Paternity Use Only.
Important Notice
The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.
Quantifiler™ Human DNA Quantification Kit (Applied Biosystems™)
Ce kit avec PCR en temps réel est conçu pour la quantification de l’ADN humain amplifiable présent au sein d’un échantillon. Il a été homologué pour les applications d’identification humaine. Un contrôle PCR interne est formulé au sein de chaque réaction afin de permettre l’identification d’échantillons susceptibles de contenir des inhibiteurs de la PCR.
• Quantification de l’ADN provenant de sources diverses et variées (notamment : prélèvements buccaux, sang, sperme, tissus et autres sources d’ADN humain), ce qui fait de ce kit un allié idéal pour les études de cas, la constitution de bases de données et la détermination de paternité.
• Compatibilité chimique avec les technologies d’extraction les plus courantes, afin de permettre une intégration aisée au sein des flux de travaux actuels du laboratoire.
• Présence d’un contrôle PCR interne pré-formulé au sein de chaque réaction afin de permettre de contrôler rapidement la bonne exécution de l’amplification et d’identifier les échantillons susceptibles de contenir des inhibiteurs.
• La PCR en temps réel possède la plus grande échelle dynamique de toutes les technologies de détection de l’ADN, offrant ainsi une quantification plus précise d’échantillons à faible concentration.
• Quantification d’une large plage de concentrations d’ADN, allant de 0,023 ng⁄ul à >50 ng⁄ul, permettant ainsi l’utilisation d’une vaste plage de concentrations d’ADN.
Minimisation du nombre d’analyses STR
Ce kit peut être facilement intégré à un flux de travaux d’analyse des séquences courtes répétées en tandem (STR). Il permet de prédire de façon plus précise la quantité d’ADN nécessaire à une amplification STR optimale.
Format à tube unique, facile à utiliser
La réalisation au sein d’un seul et même tube de la quantification et de la réaction PCR permet de minimiser la contamination.
Fiabilité de la technologie TaqMan®
Des essais biologiques basés sur la technologie TaqMan® sont utilisés dans l’ensemble de la communauté scientifique pour les essais de découverte scientifique tels que l’expression génétique, la détection d’ADN, la détermination de la présence ou de l’absence d’acides nucléiques et le génotypage SNP. Les kits Quantifiler® s’appuient sur cette technologie à la réputation et à la fiabilité désormais établie à des fins de quantification de l’ADN masculin total (voir la figure 1).
Experience Matters™
Un partenariat avec Applied Biosystems® vous permet de bénéficier de toutes les connaissances et de toute l’expérience de notre équipe d’assistance médico-légale, ainsi que de nos ressources de formations dans ce domaine. Notre université HID assure la formation continue des professionnels de l’ADN. Nos scientifiques dédiés à l’assistance et aux applications mettent à votre disposition plus de 100 ans d’expérience combinée dans le domaine de la médecine légale ; notre vaste réseau de techniciens d’entretien sur site, quant à lui, vous offre une assistance technique pour tous vos instruments.
• Quantification de l’ADN provenant de sources diverses et variées (notamment : prélèvements buccaux, sang, sperme, tissus et autres sources d’ADN humain), ce qui fait de ce kit un allié idéal pour les études de cas, la constitution de bases de données et la détermination de paternité.
• Compatibilité chimique avec les technologies d’extraction les plus courantes, afin de permettre une intégration aisée au sein des flux de travaux actuels du laboratoire.
• Présence d’un contrôle PCR interne pré-formulé au sein de chaque réaction afin de permettre de contrôler rapidement la bonne exécution de l’amplification et d’identifier les échantillons susceptibles de contenir des inhibiteurs.
• La PCR en temps réel possède la plus grande échelle dynamique de toutes les technologies de détection de l’ADN, offrant ainsi une quantification plus précise d’échantillons à faible concentration.
• Quantification d’une large plage de concentrations d’ADN, allant de 0,023 ng⁄ul à >50 ng⁄ul, permettant ainsi l’utilisation d’une vaste plage de concentrations d’ADN.
Minimisation du nombre d’analyses STR
Ce kit peut être facilement intégré à un flux de travaux d’analyse des séquences courtes répétées en tandem (STR). Il permet de prédire de façon plus précise la quantité d’ADN nécessaire à une amplification STR optimale.
Format à tube unique, facile à utiliser
La réalisation au sein d’un seul et même tube de la quantification et de la réaction PCR permet de minimiser la contamination.
Fiabilité de la technologie TaqMan®
Des essais biologiques basés sur la technologie TaqMan® sont utilisés dans l’ensemble de la communauté scientifique pour les essais de découverte scientifique tels que l’expression génétique, la détection d’ADN, la détermination de la présence ou de l’absence d’acides nucléiques et le génotypage SNP. Les kits Quantifiler® s’appuient sur cette technologie à la réputation et à la fiabilité désormais établie à des fins de quantification de l’ADN masculin total (voir la figure 1).
Experience Matters™
Un partenariat avec Applied Biosystems® vous permet de bénéficier de toutes les connaissances et de toute l’expérience de notre équipe d’assistance médico-légale, ainsi que de nos ressources de formations dans ce domaine. Notre université HID assure la formation continue des professionnels de l’ADN. Nos scientifiques dédiés à l’assistance et aux applications mettent à votre disposition plus de 100 ans d’expérience combinée dans le domaine de la médecine légale ; notre vaste réseau de techniciens d’entretien sur site, quant à lui, vous offre une assistance technique pour tous vos instruments.
AmpFLSTR™ Yfiler™ PCR Amplification Kit (Applied Biosystems™)
The AmpFLSTR® Yfiler® PCR Amplification Kit permits the analysis of complex male:female DNA mixtures that often yield ambiguous or inconclusive results with autosomal STRs.
Key Features of the AmpFLSTR® Yfiler® PCR Amplification Kit:
• Analyze trace quantities of male DNA that may be present in a large background of female DNA
• Combine the inherent male specificity of Y-STRs with the robust performance of an AmpFLSTR® kit
• Amplify 17 Y-STR loci in a single PCR reaction which yields the highest discriminatory capacity of any commercially available Y-STR kit
• Search the user-friendly on-line haplotype database to easily determine the frequency of any Yfiler® kit haplotype
• Use this powerful tool for the analysis of cases that contain a suspect(s)
Combines the Inherent Male Specificity of Y-STRs with the Robust Performance of an AmpFLSTR® Kit
Mixtures of body fluids and/or tissues from a male and female are frequently encountered with sexual assault casework and other violent crimes. These mixed samples often contain trace quantities of male DNA present in a large background of female DNA. Typical samples can include vaginal swabs containing a small number of sperm cells, body swabs from female victims of oral assaults, and fingernail scrapings/clippings from female victims of violent assaults. Standard autosomal STR analysis may be unable to detect the minor male contributor in these types of samples, while the Yfiler® kit can routinely detect a minor male contributor present at less than 1:1000.
Provides the Highest Discriminatory Capacity of Any Commercially Available Y-STR Kit
The AmpFLSTR® Yfiler® PCR Amplification Kit amplifies 17 Y-STR loci in a single PCR reaction using 5-dye chemistry to provide the highest discriminatory capacity of any commercially available Y-STR kit. The Yfiler® kit can distinguish more than 98% of all unrelated males from one another, and therefore offers the discriminatory power necessary to conduct meaningful forensic DNA analysis.
A Powerful Tool for Suspect Casework
The Yfiler® kit is a powerful tool for the analysis of cases that contain a suspect(s). A significant proportion of DNA cases contain a suspect who has been identified by conventional law enforcement techniques. For these cases, the Yfiler® kit is a powerful tool that can be used to include or exclude a suspect(s) associated with an investigation.
Complete Validated Solution
Applied Biosystems® offers a complete solution for your Human Identification needs. Our system includes DNA quantification kits, a range of AmpFLSTR® kits, instrumentation, software, and consumables. The Yfiler® kit is part of the most comprehensively validated systems for STR-based Human Identification. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations such as SWGDAM and DAB.
For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.
Key Features of the AmpFLSTR® Yfiler® PCR Amplification Kit:
• Analyze trace quantities of male DNA that may be present in a large background of female DNA
• Combine the inherent male specificity of Y-STRs with the robust performance of an AmpFLSTR® kit
• Amplify 17 Y-STR loci in a single PCR reaction which yields the highest discriminatory capacity of any commercially available Y-STR kit
• Search the user-friendly on-line haplotype database to easily determine the frequency of any Yfiler® kit haplotype
• Use this powerful tool for the analysis of cases that contain a suspect(s)
Combines the Inherent Male Specificity of Y-STRs with the Robust Performance of an AmpFLSTR® Kit
Mixtures of body fluids and/or tissues from a male and female are frequently encountered with sexual assault casework and other violent crimes. These mixed samples often contain trace quantities of male DNA present in a large background of female DNA. Typical samples can include vaginal swabs containing a small number of sperm cells, body swabs from female victims of oral assaults, and fingernail scrapings/clippings from female victims of violent assaults. Standard autosomal STR analysis may be unable to detect the minor male contributor in these types of samples, while the Yfiler® kit can routinely detect a minor male contributor present at less than 1:1000.
Provides the Highest Discriminatory Capacity of Any Commercially Available Y-STR Kit
The AmpFLSTR® Yfiler® PCR Amplification Kit amplifies 17 Y-STR loci in a single PCR reaction using 5-dye chemistry to provide the highest discriminatory capacity of any commercially available Y-STR kit. The Yfiler® kit can distinguish more than 98% of all unrelated males from one another, and therefore offers the discriminatory power necessary to conduct meaningful forensic DNA analysis.
A Powerful Tool for Suspect Casework
The Yfiler® kit is a powerful tool for the analysis of cases that contain a suspect(s). A significant proportion of DNA cases contain a suspect who has been identified by conventional law enforcement techniques. For these cases, the Yfiler® kit is a powerful tool that can be used to include or exclude a suspect(s) associated with an investigation.
Complete Validated Solution
Applied Biosystems® offers a complete solution for your Human Identification needs. Our system includes DNA quantification kits, a range of AmpFLSTR® kits, instrumentation, software, and consumables. The Yfiler® kit is part of the most comprehensively validated systems for STR-based Human Identification. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations such as SWGDAM and DAB.
For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.
AmpFLSTR™ NGM™ PCR Amplification Kit (Applied Biosystems™)
The NGM™ Kit is a highly robust Short Tandem Repeat (STR) multiplex kit which utilizes a single protocol to amplify the 10 SGM Plus™ loci (D2S1338, D3S1358, D8S1179, D16S539, D18S51, D19S433, D21S11, FGA, TH01, vWA, Amelogenin) together with the additional loci approved by the European Union Council for the expansion of the European Standard Set (D10S1248, D22S1045, D2S441, D1S1656 & D12S391). The kit combines the latest advances in buffer optimization, primer synthesis and thermal cycling optimization to deliver high sensitivity and improved STR performance for forensic casework and database samples in one easy workflow.
Key Features of the NGM™ Kit:
• Simultaneous amplification of the SGM Plus® kit loci plus 5 additional ENFSI/EDNAP recommended loci to provide powerful discrimination and facilitate cross-border data sharing
• Unprecedented performance in the presence of significant inhibitors combined with enhanced profile and locus balance
• Increased ability to recover information from degraded samples through a concentration of highly discriminatory loci in the low molecular weight region
• Greater sensitivity for improved amplification of low-level samples and better detection of the minor contributor in mixed samples
• Exceptional profile quality and clean baseline resulting from improved primer synthesis and purification processes
• 100% concordance with previously typed samples through maintenance of all STR primer sequences for loci common to the SGM Plus®, Identifiler® and SEfiler Plus™ Kits
• Proprietary and proven mobility modifier technology enables optimal locus spacing
• One kit, one protocol delivers high quality results from both database and casework samples
• Streamlined workflow with reduced amplification time, enzyme pre-formulated within the master mix and a larger package size option (1000 reactions) for high-throughput laboratories
• Optimized as part of an integrated reagent, instrumentation and software system for maximum performance and efficiency
• Outstanding discrimination and robustness offers utility for specialized applications including complex paternity/kinship, disaster victim identification and missing persons cases
Note: For Forensic or Paternity Use Only.
Important Notice
The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.
For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.
Key Features of the NGM™ Kit:
• Simultaneous amplification of the SGM Plus® kit loci plus 5 additional ENFSI/EDNAP recommended loci to provide powerful discrimination and facilitate cross-border data sharing
• Unprecedented performance in the presence of significant inhibitors combined with enhanced profile and locus balance
• Increased ability to recover information from degraded samples through a concentration of highly discriminatory loci in the low molecular weight region
• Greater sensitivity for improved amplification of low-level samples and better detection of the minor contributor in mixed samples
• Exceptional profile quality and clean baseline resulting from improved primer synthesis and purification processes
• 100% concordance with previously typed samples through maintenance of all STR primer sequences for loci common to the SGM Plus®, Identifiler® and SEfiler Plus™ Kits
• Proprietary and proven mobility modifier technology enables optimal locus spacing
• One kit, one protocol delivers high quality results from both database and casework samples
• Streamlined workflow with reduced amplification time, enzyme pre-formulated within the master mix and a larger package size option (1000 reactions) for high-throughput laboratories
• Optimized as part of an integrated reagent, instrumentation and software system for maximum performance and efficiency
• Outstanding discrimination and robustness offers utility for specialized applications including complex paternity/kinship, disaster victim identification and missing persons cases
Note: For Forensic or Paternity Use Only.
Important Notice
The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.
For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.
