Shop All Library Preparation Kits for Sequencing
Ion AmpliSeq™ RNA Apoptosis Panel (Ion Torrent™)
The Ion AmpliSeq™ RNA Apoptosis Panel is a screening tool designed against 267 genes involved in the cellular apoptosis pathway. Included are genes associated with death receptor-mediated apoptosis, c-Myc, and p53-mediated apoptosis.
• Measure gene expression fold changes between samples
• Amplicons are designed to be most inclusive for transcripts of the target gene
• Each reaction requires only 500 pg of unfixed RNA or 5 ng of fixed (FFPE) RNA
• Prepare amplicon libraries with the Ion AmpliSeq™ RNA Library Kit and sequence on the Ion Personal Genome Machine® (PGM™) or Ion Proton™ system
This panel contains 267 amplicons (534 primers), approximately 150 bases in length, in a single pool.
• Measure gene expression fold changes between samples
• Amplicons are designed to be most inclusive for transcripts of the target gene
• Each reaction requires only 500 pg of unfixed RNA or 5 ng of fixed (FFPE) RNA
• Prepare amplicon libraries with the Ion AmpliSeq™ RNA Library Kit and sequence on the Ion Personal Genome Machine® (PGM™) or Ion Proton™ system
This panel contains 267 amplicons (534 primers), approximately 150 bases in length, in a single pool.
SOLiD™ RNA Barcoding Kit, Module 1-16 (Applied Biosystems™)
The SOLiD™ RNA Barcoding Kit, Module 1-16 enables you to increase the power of your studies by cost effectively increasing the numbers of samples analyzed in one SOLiD™ Sequencing run. Compatible with the SOLiD™ Total RNA-Seq Kit and SOLiD™ SAGE™ Kit, the SOLiD™ System barcodes contain unique sequences designed for optimal multiplexing. Module 1-16 contains sixteen different barcodes, selected for uniform melting temperature, low error rate, and orthogonal sequences that are unique in color space. The barcodes are added to the 3' end of the target sequence using a modified P2 adapter. SOLiD™ System barcoding enables the assignment of a unique identifier to templated beads made from a single library. Once the identifiers are assigned, multiple batches of templated beads may be pooled together for emulsion PCR and then sequenced.
Following sequencing, additional rounds of ligation-based sequencing are performed using primer sets complimentary to the barcode. The resulting reads can then be sorted by the barcode and aligned in groups to the reference sequence, enabling the sequencing of multiple libraries on the same slide—maximizing throughput while dramatically reducing costs.
SOLiD™ RNA Barcoding Kits contain PCR primer sets that are designed for use with the SOLiD™ Total RNA-Seq Kit and SOLiD™ SAGE™ Kit. Together, these kits enable multiplex SOLiD™ System sequencing of RNA samples. The SOLiD™ Total RNA-Seq Kit contains two primers:
• The SOLiD™ 5' PCR Primer is identical to the 5’ PCR primer provided in the SOLiD™ Total RNA-Seq Kit, and it contains the SOLiD™ emulsion PCR primer P1 sequence.
• The SOLiD™ 3' (reverse) PCR primers contain the P2 sequence required for SOLiD™ emulsion PCR, along with a unique barcode sequence, and an internal adaptor (IA) sequence necessary for sequencing the barcode. Using these primers in the cDNA amplification step of the SOLiD™ Total RNA-Seq Kit procedure generates barcoded cDNA libraries that can be mixed for multiplex SOLiD™ System sequencing.
Following sequencing, additional rounds of ligation-based sequencing are performed using primer sets complimentary to the barcode. The resulting reads can then be sorted by the barcode and aligned in groups to the reference sequence, enabling the sequencing of multiple libraries on the same slide—maximizing throughput while dramatically reducing costs.
SOLiD™ RNA Barcoding Kits contain PCR primer sets that are designed for use with the SOLiD™ Total RNA-Seq Kit and SOLiD™ SAGE™ Kit. Together, these kits enable multiplex SOLiD™ System sequencing of RNA samples. The SOLiD™ Total RNA-Seq Kit contains two primers:
• The SOLiD™ 5' PCR Primer is identical to the 5’ PCR primer provided in the SOLiD™ Total RNA-Seq Kit, and it contains the SOLiD™ emulsion PCR primer P1 sequence.
• The SOLiD™ 3' (reverse) PCR primers contain the P2 sequence required for SOLiD™ emulsion PCR, along with a unique barcode sequence, and an internal adaptor (IA) sequence necessary for sequencing the barcode. Using these primers in the cDNA amplification step of the SOLiD™ Total RNA-Seq Kit procedure generates barcoded cDNA libraries that can be mixed for multiplex SOLiD™ System sequencing.
Precision ID Library Kit (Ion Torrent™)
The Precision ID Library Kit is designed for rapid preparation of amplicon libraries using HID-Ion AmpliSeq ready-to-use panels, for sequencing on Ion Torrent sequencing system. This kit uses only certain components found in the Ion AmpliSeq™ Library Kit 2.0, that are needed for Precision ID applications, making the Precision ID Library Kit more customized and specific for forensic research use only.
Key features:
Simple protocol
• 96 well plate-based workflow allows easy sample management
• No library amplification with qPCR quantification
Ion AmpliSeq™ HiFi Master Mix
• Enables cleaner amplification for increased coverage uniformity
• Up to 3,072-plex PCR allows larger panel designs
Digestion reagent
• Cleaner digest allows for more efficient downstream sequencing
Benefits for HID applications:
• 1 ng of DNA input is recommended for the Precision ID panels
• Less components to help save time and money by preventing unnecessary waste
Scalable multiplex PCR reactions
The Precision ID Library Kit is built on Ion AmpliSeq chemistry, which enables scalable multiplex PCR reactions from 12- to 3,072-plex in a single well using just 1 ng of starting DNA. Ion AmpliSeq panels and primer pools allow highly multiplexed PCR amplification of thousands of genomic target regions, with superior coverage uniformity and specificity, without the need of a specialized microfluidics PCR platform. In addition, the primers contain proprietary modifications that enable removal of primer sequences during library preparation, for efficient target assessment during sequencing. Multiple primer pools can be used to create overlapping amplicons that enable complete coverage of large targets.
Barcoded library preparation
The Precision ID Library Kit includes reagents for generating amplicons with Ion AmpliSeq primers and preparing libraries from the resulting amplicons. The kit enables the preparation of barcoded libraries using Ion Xpress™ Barcode Adapters 1–96 kits and the IonCode™ Barcode Adapters 1-384 Kit. Barcoded libraries can be combined and loaded onto a single Ion chip to minimize the sequencing run time and cost and allow for accurate sample-to-sample comparisons.
Easy and flexible protocol
The Precision ID Library Kit uses a plate-based format for easier sample handling and tracking, and for compatibility with automation and high-throughput laboratories.
The resulting DNA libraries are ready for downstream template preparation for clonal amplification on Ion Sphere™ particles using the automated Ion OneTouch™ 2 System. The intuitive Torrent Suite Software and HID SNP or STR Genotyper plug-ins enable you to go from extracted DNA to ancestry-informative genotypes in less than two days*.
*Using the Applied Biosystems™ Precision ID NGS System for Human Identification
Key features:
Simple protocol
• 96 well plate-based workflow allows easy sample management
• No library amplification with qPCR quantification
Ion AmpliSeq™ HiFi Master Mix
• Enables cleaner amplification for increased coverage uniformity
• Up to 3,072-plex PCR allows larger panel designs
Digestion reagent
• Cleaner digest allows for more efficient downstream sequencing
Benefits for HID applications:
• 1 ng of DNA input is recommended for the Precision ID panels
• Less components to help save time and money by preventing unnecessary waste
Scalable multiplex PCR reactions
The Precision ID Library Kit is built on Ion AmpliSeq chemistry, which enables scalable multiplex PCR reactions from 12- to 3,072-plex in a single well using just 1 ng of starting DNA. Ion AmpliSeq panels and primer pools allow highly multiplexed PCR amplification of thousands of genomic target regions, with superior coverage uniformity and specificity, without the need of a specialized microfluidics PCR platform. In addition, the primers contain proprietary modifications that enable removal of primer sequences during library preparation, for efficient target assessment during sequencing. Multiple primer pools can be used to create overlapping amplicons that enable complete coverage of large targets.
Barcoded library preparation
The Precision ID Library Kit includes reagents for generating amplicons with Ion AmpliSeq primers and preparing libraries from the resulting amplicons. The kit enables the preparation of barcoded libraries using Ion Xpress™ Barcode Adapters 1–96 kits and the IonCode™ Barcode Adapters 1-384 Kit. Barcoded libraries can be combined and loaded onto a single Ion chip to minimize the sequencing run time and cost and allow for accurate sample-to-sample comparisons.
Easy and flexible protocol
The Precision ID Library Kit uses a plate-based format for easier sample handling and tracking, and for compatibility with automation and high-throughput laboratories.
The resulting DNA libraries are ready for downstream template preparation for clonal amplification on Ion Sphere™ particles using the automated Ion OneTouch™ 2 System. The intuitive Torrent Suite Software and HID SNP or STR Genotyper plug-ins enable you to go from extracted DNA to ancestry-informative genotypes in less than two days*.
*Using the Applied Biosystems™ Precision ID NGS System for Human Identification
MuSeek Index Set 1, Illumina compatible (Thermo Scientific™)
Thermo Scientific MuSeek Index Set 1, Illumina™ compatible, is designed for generation of indexed genomic DNA libraries for sequencing on the Illumina MiSeq™ and HiSeq™ instruments. The set provides 4 indexed P5 adapters and 6 MuSeek indexed P7 adapters. This enables pooling of up to 24 dual-indexed libraries and conducting multiplexed sequencing analysis. The MuSeek Index Set 1, in combination with MuSeek Library Preparation kit, Illumina™ compatible, allows fast and simple indexed library construction from 50 ng of sample DNA, eliminating the need for physical shearing, end conversion and adapter ligation steps.
Highlights
• Enables sequencing of up to 24 dual-indexed fragment libraries in a single sequencing run.
Applications
• Indexed DNA fragment library preparation for next generation sequencing.
Includes
• MuSeek Indices M501-4
• MuSeek Indices M701-6
Highlights
• Enables sequencing of up to 24 dual-indexed fragment libraries in a single sequencing run.
Applications
• Indexed DNA fragment library preparation for next generation sequencing.
Includes
• MuSeek Indices M501-4
• MuSeek Indices M701-6
Ion AmpliSeq™ RNA Cancer Panel (Ion Torrent™)
The Ion AmpliSeq™ RNA Cancer Panel is a screening tool designed against 50 oncogenes/tumor suppressor genes found in the COSMIC database. This panel has been developed as an RNA complement to the DNA-focused Ion AmpliSeq™ Cancer Hotspot Panel v2. The panel is a single pool of primers representing 50 oncogenes and tumor suppressor genes with coverage of KRAS, BRAF, and EGFR genes.
• Measure gene expression fold changes between samples
• Amplicons are designed to be most inclusive for transcripts of the target gene
• Each reaction requires only 500 pg of unfixed RNA or 5 ng of fixed (FFPE) RNA
• Prepare amplicon libraries with the Ion AmpliSeq™ RNA Library Kit and sequence on the Ion Personal Genome Machine® (PGM™) or Ion Proton™ system
This panel contains 50 amplicons (100 primers), approximately 150 bases in length, in a single pool.
• Measure gene expression fold changes between samples
• Amplicons are designed to be most inclusive for transcripts of the target gene
• Each reaction requires only 500 pg of unfixed RNA or 5 ng of fixed (FFPE) RNA
• Prepare amplicon libraries with the Ion AmpliSeq™ RNA Library Kit and sequence on the Ion Personal Genome Machine® (PGM™) or Ion Proton™ system
This panel contains 50 amplicons (100 primers), approximately 150 bases in length, in a single pool.
Ion Xpress™ Barcode Adapters 49-64 Kit (Ion Torrent™)
The Ion Xpress™ Barcode Adapters 49-64 Kit provides a set of 16 unique barcode adaptors specifically designed and validated for optimal performance with the Ion Torrent PGM™ and Proton™ semiconductor sequencers. When used in combination with the Ion Xpress™ Plus Fragment Library Kit or the Ion Plus Fragment Library Kit, this kit enables users to pool up to 16 fragment libraries prior to emulsion PCR and then conduct multiplexed sequencing analysis, simplifying the Ion semiconductor sequencing workflow for a wide range of applications, including targeted enrichment. Use of this kit with other Barcode Adapters kits allows pooling of up to 96 fragment libraries.
Key Product Features:
• Enables multiplexing of numerous fragment library samples on a single sequencing chip with the use of robust molecular barcodes
• Both sequence- and flow-optimized for equal representation all barcodes in a pool and more economical multiplexed sequencing runs
• Robust error correction built in by design for added confidence in sample identification
Multiplexing with Barcode Adaptors Enables Higher Throughput
Multiplexing with barcoded libraries leads to more cost effective runs by permitting up to 96 samples per run (16 with this kit), significantly decreasing the cost and handling requirements of emPCR, enrichment, and sequencing.
Sequence- and Flow-Optimized for Increased Performance and Efficiency
Barcode adapters contain sequences that are optimized to provide equal representation of all barcodes in a pool, require a minimum number of flows to interrogate, and require only 17 bases of adaptor sequencing required which results in more economical multiplexed sequencing runs.
Robust Error Correction Built In by Design
Built-in error correction virtually eliminates the possibility of incorrect sample assignment.
For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
Key Product Features:
• Enables multiplexing of numerous fragment library samples on a single sequencing chip with the use of robust molecular barcodes
• Both sequence- and flow-optimized for equal representation all barcodes in a pool and more economical multiplexed sequencing runs
• Robust error correction built in by design for added confidence in sample identification
Multiplexing with Barcode Adaptors Enables Higher Throughput
Multiplexing with barcoded libraries leads to more cost effective runs by permitting up to 96 samples per run (16 with this kit), significantly decreasing the cost and handling requirements of emPCR, enrichment, and sequencing.
Sequence- and Flow-Optimized for Increased Performance and Efficiency
Barcode adapters contain sequences that are optimized to provide equal representation of all barcodes in a pool, require a minimum number of flows to interrogate, and require only 17 bases of adaptor sequencing required which results in more economical multiplexed sequencing runs.
Robust Error Correction Built In by Design
Built-in error correction virtually eliminates the possibility of incorrect sample assignment.
For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
Ion AmpliSeq™ Exome RDY Kit 4x2 (Ion Torrent™)
The Ion AmpliSeq™ Exome RDY Kit enables the fastest and simplest method of exome enrichment, now even easier with oligo pools for ultra-high multiplex PCR exome enrichment dried down in 96-well plates. With half the number of target amplification pipetting steps compared to the original protocol and the elimination of small pipetting volumes, exome libraries can now be produced with unparalleled ease in less than 6 hours with less than 50 minutes of hands-on time. Also, the Ion AmpliSeq™ Exome RDY Kit is compatible with the Ion Library Equalizer™ Kit for ease in library normalization. Leveraging the ultra-high multiplex PCR approach of Ion AmpliSeq™ technology with template preparation on the Ion Chef™ System, along with the Ion Proton™ Sequencer, the Ion Ion AmpliSeq™ Exome RDY Kit allows for rapid sequencing of key exonic regions of the genome, going from DNA to variants in just 2 days.
The Ion AmpliSeq™ Exome RDY Kit 4x2 configuration includes the following components for 8 exomes (running 2 exomes per Ion PI™ Chip v3):
• Ion AmpliSeq™ Exome RDY Panel: dried down oligo pools/primers in four 96-well plates, each with 2 rows(C and F) filled (4x2), for ultra-high multiplex PCR enrichment of the exonic regions of the genome
• Ion AmpliSeq™ Library Kit Plus (8 exomes) for robust library preparation of Ion AmpliSeq™ exome libraries using Ion AmpliSeq™ technology
• Ion PI™ Chip Kit v3 for sequencing on the Ion Proton™ Sequencer
The Ion Ion AmpliSeq™ Exome RDY Kit enables you to:
• Simplify exome enrichment through the combination of dried-down primers and the Ion AmpliSeq™ workflow, resulting in less than 1 hour of hands-on time to produce exome libraries in under 6 hours
• Accelerate your disease research with rapid exome sequencing, going from DNA to variants in just 2 days
• Access affordable exome sequencing when you need it, without the need to batch dozens of samples or the high price of a lower-throughput run
• Maximize sequencing efficiency with >90% on-target bases and >92% coverage uniformity, and design covering >97% of the Consensus Coding Sequences (CCDS) annotation
• Identify annotated, filtered variants without bioinformatics expertise through fully integrated analysis with Torrent Suite™ and Ion Reporter™ software
Quickest exome enrichment with unprecedented simplicity
The Ion Ion AmpliSeq™ Exome RDY Kit provides unmatched simplicity for exome enrichment. The same primers used in the Ion AmpliSeq™ Exome Kit 4xDuo and Ion AmpliSeq™ Exome Kit 8xTrio have been dried down into a 96-well plate, using the 12 wells of a single row for each of the 12 primer pools, leading to the use of 1 row per exome. The Ion Ion AmpliSeq™ Exome RDY Kit 4x2 configuration contains four 96-well plates, each with 2 rows pre-filled with dried down primers, sufficient to prepare 8 exome libraries. Additionally, the dried down primers contain a light blue dye for easy identification of used or occupied rows. The number of target amplification pipetting steps is reduced by 46%, and small pipetting volumes (<5 µL) have been eliminated. With a workflow requiring less than 50 minutes of hands-on time and no centrifugation steps, the kit enables exome library preparation in less than 6 hours with the least amount of hands-on time. Additionally, the Ion Ion AmpliSeq™ Exome RDY Kit maintains a low input requirement of 50 ng DNA. With the included Ion AmpliSeq™ Library Kit Plus, exome library preparation is more robust, resulting in higher uniformity and consistently higher library yield when performing library amplification. Also, the Ion AmpliSeq™ Exome RDY Kit is now compatible with the Ion Library Equalizer™ Kit for ultimate ease in library normalization.
Lowest cost per exome using a benchtop sequencer
Paired with the Ion Proton™ System, the Ion AmpliSeq™ Exome RDY Kit allows disease researchers to access affordable exome sequencing on their benchtops without long turn-around times or the sample batching normally required to achieve a low cost per sample. Using the Ion Ion AmpliSeq™ Exome RDY Kit with the Ion Proton™ System enables you to go from DNA to variants in as little as 2 days. Additionally, with the most uniform exome enrichment covering >97% of the CCDS, you can achieve the most efficient sequencing with 2 exomes run per Ion PI™ chip.
Fully integrated analysis without bioinformatics expertise
Use of the kit with Torrent Suite™ and Ion Reporter™ software provides a simple and streamlined analysis workflow that automates the identification of your variants of interest without needing the expertise or infrastructure of a full bioinformatics team. Additionally, the Ion Proton™ Sequencer along with Ion Reporter™ Software deliver both SNP and indel information along with copy number changes through a single analysis workflow, advancing your human disease research.
Notes:
This kit is compatible with the Ion Chef™, Ion OneTouch™ 2, and Ion Proton™ systems.
The Ion AmpliSeq™ Exome RDY Kit 4x2 configuration includes the following components for 8 exomes (running 2 exomes per Ion PI™ Chip v3):
• Ion AmpliSeq™ Exome RDY Panel: dried down oligo pools/primers in four 96-well plates, each with 2 rows(C and F) filled (4x2), for ultra-high multiplex PCR enrichment of the exonic regions of the genome
• Ion AmpliSeq™ Library Kit Plus (8 exomes) for robust library preparation of Ion AmpliSeq™ exome libraries using Ion AmpliSeq™ technology
• Ion PI™ Chip Kit v3 for sequencing on the Ion Proton™ Sequencer
The Ion Ion AmpliSeq™ Exome RDY Kit enables you to:
• Simplify exome enrichment through the combination of dried-down primers and the Ion AmpliSeq™ workflow, resulting in less than 1 hour of hands-on time to produce exome libraries in under 6 hours
• Accelerate your disease research with rapid exome sequencing, going from DNA to variants in just 2 days
• Access affordable exome sequencing when you need it, without the need to batch dozens of samples or the high price of a lower-throughput run
• Maximize sequencing efficiency with >90% on-target bases and >92% coverage uniformity, and design covering >97% of the Consensus Coding Sequences (CCDS) annotation
• Identify annotated, filtered variants without bioinformatics expertise through fully integrated analysis with Torrent Suite™ and Ion Reporter™ software
Quickest exome enrichment with unprecedented simplicity
The Ion Ion AmpliSeq™ Exome RDY Kit provides unmatched simplicity for exome enrichment. The same primers used in the Ion AmpliSeq™ Exome Kit 4xDuo and Ion AmpliSeq™ Exome Kit 8xTrio have been dried down into a 96-well plate, using the 12 wells of a single row for each of the 12 primer pools, leading to the use of 1 row per exome. The Ion Ion AmpliSeq™ Exome RDY Kit 4x2 configuration contains four 96-well plates, each with 2 rows pre-filled with dried down primers, sufficient to prepare 8 exome libraries. Additionally, the dried down primers contain a light blue dye for easy identification of used or occupied rows. The number of target amplification pipetting steps is reduced by 46%, and small pipetting volumes (<5 µL) have been eliminated. With a workflow requiring less than 50 minutes of hands-on time and no centrifugation steps, the kit enables exome library preparation in less than 6 hours with the least amount of hands-on time. Additionally, the Ion Ion AmpliSeq™ Exome RDY Kit maintains a low input requirement of 50 ng DNA. With the included Ion AmpliSeq™ Library Kit Plus, exome library preparation is more robust, resulting in higher uniformity and consistently higher library yield when performing library amplification. Also, the Ion AmpliSeq™ Exome RDY Kit is now compatible with the Ion Library Equalizer™ Kit for ultimate ease in library normalization.
Lowest cost per exome using a benchtop sequencer
Paired with the Ion Proton™ System, the Ion AmpliSeq™ Exome RDY Kit allows disease researchers to access affordable exome sequencing on their benchtops without long turn-around times or the sample batching normally required to achieve a low cost per sample. Using the Ion Ion AmpliSeq™ Exome RDY Kit with the Ion Proton™ System enables you to go from DNA to variants in as little as 2 days. Additionally, with the most uniform exome enrichment covering >97% of the CCDS, you can achieve the most efficient sequencing with 2 exomes run per Ion PI™ chip.
Fully integrated analysis without bioinformatics expertise
Use of the kit with Torrent Suite™ and Ion Reporter™ software provides a simple and streamlined analysis workflow that automates the identification of your variants of interest without needing the expertise or infrastructure of a full bioinformatics team. Additionally, the Ion Proton™ Sequencer along with Ion Reporter™ Software deliver both SNP and indel information along with copy number changes through a single analysis workflow, advancing your human disease research.
Notes:
This kit is compatible with the Ion Chef™, Ion OneTouch™ 2, and Ion Proton™ systems.
Ion SingleSeq™ 96 Kit (Ion Torrent™)
The Ion SingleSeq 96 Kit is designed for low cell number input library construction prior to low-pass genome sequencing detection of copy number events. Requiring as little as 6 pg of DNA from a single cell, or input from multiple cells, this kit includes reagents to extract, amplify, and barcode genomic DNA for preparation of libraries from up to 96 samples.
The Ion SingleSeq 96 Kit is intended to be used with the Ion ReproSeq PGS Kit for the Ion S5 System for the detection of chromosomal aneuploidies and chromosome arm copy number events (>48 Mb). The Ion ReproSeq PGS Kit is used with the Ion Chef System and the Ion S5 or Ion S5 XL sequencing system to enable automated template preparation, chip loading, and sequencing with disposable reagent cartridges for fast and simple instrument set-up to minimize hands-on time. Compatible kits include:
• Ion ReproSeq PGS Kit with Ion 520 Chips (24 samples/run) (Cat. No. A34900)
• Ion ReproSeq PGS Kit with Ion 530 Chips (96 samples/run) (Cat. No. A34901)
The Ion SingleSeq 96 Kit is intended to be used with the Ion ReproSeq PGS Kit for the Ion S5 System for the detection of chromosomal aneuploidies and chromosome arm copy number events (>48 Mb). The Ion ReproSeq PGS Kit is used with the Ion Chef System and the Ion S5 or Ion S5 XL sequencing system to enable automated template preparation, chip loading, and sequencing with disposable reagent cartridges for fast and simple instrument set-up to minimize hands-on time. Compatible kits include:
• Ion ReproSeq PGS Kit with Ion 520 Chips (24 samples/run) (Cat. No. A34900)
• Ion ReproSeq PGS Kit with Ion 530 Chips (96 samples/run) (Cat. No. A34901)
Oncomine™ Colon cfDNA Assay (Ion Torrent™)
The Oncomine™ Colon cfDNA Assay is part of a complete solution to detect colon (or other related gastro-intestinal) tumor-derived DNA (ctDNA) in cell-free DNA (cfDNA). It provides the reagents for library construction and a single pool of multiplex PCR primers for preparation of amplicon libraries from cfDNA obtained from the plasma fraction of a single tube of whole blood. The assay enables the analysis of single nucleotide variants and short indels that are frequently mutated in colon/gastro-intestinal cancers. Fourteen genes with >240 hotspots are covered (Genes: AKT1, BRAF, CTNNB1, EGFR, ERBB2, FBXW7, GNAS, KRAS, MAP2K1, NRAS, PIK3CA, MAD4, TP53, APC). Through the use of tag sequencing technology, a limit of detection (LOD) down to 0.1% can be achieved.
Note: Target and hotspot BED files and User Guide document for cfDNA Variant Analysis with Torrent Suite Software 5.2 can be found below under "Documents".
• Optimized short amplicon design for fragmented cfDNA (~170 bp in length)
• Detection of both single nucleotide variants (SNVs) and hotspot indels down to 0.1% LOD
• Very high sensitivity and specificity, enabling extremely low levels of detection with minimal false positives
• 6 samples can be run on an Ion 530™ Chip, reducing overall sequencing costs
• Efficient workflow, from a single tube of blood to results in just 2 days
Liquid biopsies offer several advantages over conventional solid tumor biopsies:
• Liquid biopsies are less invasive, enabling samples to be taken at multiple time points to monitor progression
• Lower cost compared to traditional tissue biopsies
• Faster turnaround time from sample to results
• More tumor heterogeneity captured
The entire workflow (figure below) from isolation of cfDNA using the MagMAX™ Cell-Free DNA Isolation Kit to analysis of samples can be accomplished in just 2 days using the Ion S5™ XL sequencing system.
Technology
ctDNA is found at extremely low concentrations in the plasma fraction of whole blood. Because of this low prevalence, a tag sequencing technology is utilized in this assay. The technology attaches a unique molecular tag to the gene-specific primers. The amplified products are then grouped into families containing the same tags. Families that contain the same mutant variant will be called with optimized Variant Caller settings for the Oncology-Liquid Biopsy application. Families that contain random errors, typically generated through the library construction/sequencing process, are identified and removed from variant calling.
Unlike other technologies with LODs of 1-5%, the Oncomine Colon cfDNA Assay has a flexible detection limit down to 0.1% or 1 mutant copy in a background of 1,000 wild-type copies. To achieve 0.1% LOD, 20 ng of input cfDNA is required. Lower amounts of cfDNA can be used (down to 1 ng), but the LOD will be higher depending on the input amount.
Simplicity, speed, and scalability of tag-sequencing technology
The Oncomine Colon cfDNA Assay enables cancer genetic studies from just 1 ng of input cfDNA for targeted library construction. The cfDNA assay uses standard PCR equipment and two simple PCR reactions, one to attach the unique molecular tags and the second to amplify the library, for high multiplex PCR-based target selection with no need for extensive capital investment. Additionally, the Oncomine Colon cfDNA Assay is compatible with FFPE samples for possible concordance studies. Total time to targeted libraries is just 3.5 hours. Scalability and flexibility are achieved using the Tag Sequencing Barcode Set 1-24 (Cat. No. A31830) or Taq Sequencing Barcode Set 25-48 (Cat. No. A31847) for multiplexing barcoded samples on Ion S5 chips.
Learn more about Oncomine cfDNA Assays >
Note: Target and hotspot BED files and User Guide document for cfDNA Variant Analysis with Torrent Suite Software 5.2 can be found below under "Documents".
• Optimized short amplicon design for fragmented cfDNA (~170 bp in length)
• Detection of both single nucleotide variants (SNVs) and hotspot indels down to 0.1% LOD
• Very high sensitivity and specificity, enabling extremely low levels of detection with minimal false positives
• 6 samples can be run on an Ion 530™ Chip, reducing overall sequencing costs
• Efficient workflow, from a single tube of blood to results in just 2 days
Liquid biopsies offer several advantages over conventional solid tumor biopsies:
• Liquid biopsies are less invasive, enabling samples to be taken at multiple time points to monitor progression
• Lower cost compared to traditional tissue biopsies
• Faster turnaround time from sample to results
• More tumor heterogeneity captured
The entire workflow (figure below) from isolation of cfDNA using the MagMAX™ Cell-Free DNA Isolation Kit to analysis of samples can be accomplished in just 2 days using the Ion S5™ XL sequencing system.
Technology
ctDNA is found at extremely low concentrations in the plasma fraction of whole blood. Because of this low prevalence, a tag sequencing technology is utilized in this assay. The technology attaches a unique molecular tag to the gene-specific primers. The amplified products are then grouped into families containing the same tags. Families that contain the same mutant variant will be called with optimized Variant Caller settings for the Oncology-Liquid Biopsy application. Families that contain random errors, typically generated through the library construction/sequencing process, are identified and removed from variant calling.
Unlike other technologies with LODs of 1-5%, the Oncomine Colon cfDNA Assay has a flexible detection limit down to 0.1% or 1 mutant copy in a background of 1,000 wild-type copies. To achieve 0.1% LOD, 20 ng of input cfDNA is required. Lower amounts of cfDNA can be used (down to 1 ng), but the LOD will be higher depending on the input amount.
Simplicity, speed, and scalability of tag-sequencing technology
The Oncomine Colon cfDNA Assay enables cancer genetic studies from just 1 ng of input cfDNA for targeted library construction. The cfDNA assay uses standard PCR equipment and two simple PCR reactions, one to attach the unique molecular tags and the second to amplify the library, for high multiplex PCR-based target selection with no need for extensive capital investment. Additionally, the Oncomine Colon cfDNA Assay is compatible with FFPE samples for possible concordance studies. Total time to targeted libraries is just 3.5 hours. Scalability and flexibility are achieved using the Tag Sequencing Barcode Set 1-24 (Cat. No. A31830) or Taq Sequencing Barcode Set 25-48 (Cat. No. A31847) for multiplexing barcoded samples on Ion S5 chips.
Learn more about Oncomine cfDNA Assays >
Ion AmpliSeq™ Library Kit 2.0 (Ion Torrent™)
Le kit bibliothèque Ion AmpliSeq™ 2.0 est conçu pour préparer rapidement des bibliothèques d’amplicons en utilisant les panels et les groupes d’amorces sur mesure prêts à l’emploi Ion AmpliSeq™ pour un séquençage sur le système Ion Personal Genome Machine™ (PGM™). Cette nouvelle version “LV” du kit est configurée pour des volumes de réactifs importants, ce qui la rend idéale pour les protocoles automatiques de préparation de bibliothèques d’ADN. Le kit peut également être utilisé avec des protocoles manuels. Cette nouvelle version remplace la précédente version “non-LV” (No de catalogue 4478379).
Caractéristiques Principales :
Nouveau protocole simple
• Le flux de travaux basé sur une plaque de 96 puits permet la gestion facile des échantillons
• Une seule étape de nettoyage requise, ce qui réduit le temps de manipulation
• Pas d’amplification de bibliothèque avec l’évaluation quantitative qPCR
Nouveau mélange maître Ion AmpliSeq™ HiFi
• Permet une amplification plus propre pour une meilleure uniformité de couverture
• Une PCR allant jusqu’à 3 072 plex permet des conceptions de panneaux plus grands
Nouveau réactif de digestion
• Un produit digéré plus propre permet un séquençage plus efficace en aval
Des réactions PCR multiplex évolutives
La technologie Ion AmpliSeq™ 2.0 permet d’effectuer des réactions de PCR multiplex évolutives de 12 à 3 072 plex dans un puits unique en n’utilisant que 10 ng d’ADN de départ. Les panels et les groupes d’amorces Ion AmpliSeq™ permettent l’amplification PCR multiplexée de milliers de régions génomiques cibles, avec une uniformité et une spécificité de couverture supérieure, sans avoir besoin de recourir à une plateforme de PCR microfluidique spécialisée. De plus, les amorces contiennent des modifications exclusives qui permettent l’élimination de séquences d’amorce lors de la préparation de bibliothèques, pour une évaluation efficace des cibles lors du séquençage. Plusieurs groupes d’amorces peuvent être utilisés pour créer des amplicons se chevauchant qui permettent une couverture complète de grandes cibles Les groupes d’amorces sur mesure Ion AmpliSeq™ sont conçus par l’intermédiaire du logiciel en ligne Ion AmpliSeq™ Designer, disponible sur www.ampliseq.com.
Préparation de bibliothèques à code-barres
Le kit bibliothèque Ion AmpliSeq™ 2.0 comprend des réactifs pour la génération d’amplicons avec des amorces Ion AmpliSeq™ et la préparation de bibliothèques à partir des amplicons obtenus. Ce kit permet de préparer des bibliothèques à code-barres à l’aide des kits d’adaptateurs de code-barres Ion Xpress™ 1 à 96. Les bibliothèques à code-barres peuvent être combinées et chargées dans une seule puce Ion pour minimiser le temps et le coût d’exécution du séquençage et permettre des comparaisons précises entre échantillons.
Protocole facile et flexible
Le kit bibliothèque Ion AmpliSeq™ 2.0 utilise un format basé sur une plaque pour faciliter la manipulation et le suivi des échantillons et pour assurer la compatibilité avec les laboratoires automatisés et à haut débit. La préparation de bibliothèques est terminée en seulement 3,5 heures à l’aide de 10 ng d’ADN par PCR. Par ailleurs, de l’ADN provenant de diverses sources, notamment du tissu fixé avec de la formaline et enrobé de paraffine (FFPE), peut être utilisé comme produit de départ.
Les bibliothèques d’ADN qui en résultent sont prêtes pour la préparation d’un modèle en aval pour l’amplification clonale sur les particules Ion Sphere™ à l’aide du système automatisé Ion OneTouch™. Le logiciel Torrent Suite intuitif et le logiciel Ion Reporter en option vous permettent de passer d’ADN extrait à des classifications de variantes en à peine 10 heures.
Caractéristiques Principales :
Nouveau protocole simple
• Le flux de travaux basé sur une plaque de 96 puits permet la gestion facile des échantillons
• Une seule étape de nettoyage requise, ce qui réduit le temps de manipulation
• Pas d’amplification de bibliothèque avec l’évaluation quantitative qPCR
Nouveau mélange maître Ion AmpliSeq™ HiFi
• Permet une amplification plus propre pour une meilleure uniformité de couverture
• Une PCR allant jusqu’à 3 072 plex permet des conceptions de panneaux plus grands
Nouveau réactif de digestion
• Un produit digéré plus propre permet un séquençage plus efficace en aval
Des réactions PCR multiplex évolutives
La technologie Ion AmpliSeq™ 2.0 permet d’effectuer des réactions de PCR multiplex évolutives de 12 à 3 072 plex dans un puits unique en n’utilisant que 10 ng d’ADN de départ. Les panels et les groupes d’amorces Ion AmpliSeq™ permettent l’amplification PCR multiplexée de milliers de régions génomiques cibles, avec une uniformité et une spécificité de couverture supérieure, sans avoir besoin de recourir à une plateforme de PCR microfluidique spécialisée. De plus, les amorces contiennent des modifications exclusives qui permettent l’élimination de séquences d’amorce lors de la préparation de bibliothèques, pour une évaluation efficace des cibles lors du séquençage. Plusieurs groupes d’amorces peuvent être utilisés pour créer des amplicons se chevauchant qui permettent une couverture complète de grandes cibles Les groupes d’amorces sur mesure Ion AmpliSeq™ sont conçus par l’intermédiaire du logiciel en ligne Ion AmpliSeq™ Designer, disponible sur www.ampliseq.com.
Préparation de bibliothèques à code-barres
Le kit bibliothèque Ion AmpliSeq™ 2.0 comprend des réactifs pour la génération d’amplicons avec des amorces Ion AmpliSeq™ et la préparation de bibliothèques à partir des amplicons obtenus. Ce kit permet de préparer des bibliothèques à code-barres à l’aide des kits d’adaptateurs de code-barres Ion Xpress™ 1 à 96. Les bibliothèques à code-barres peuvent être combinées et chargées dans une seule puce Ion pour minimiser le temps et le coût d’exécution du séquençage et permettre des comparaisons précises entre échantillons.
Protocole facile et flexible
Le kit bibliothèque Ion AmpliSeq™ 2.0 utilise un format basé sur une plaque pour faciliter la manipulation et le suivi des échantillons et pour assurer la compatibilité avec les laboratoires automatisés et à haut débit. La préparation de bibliothèques est terminée en seulement 3,5 heures à l’aide de 10 ng d’ADN par PCR. Par ailleurs, de l’ADN provenant de diverses sources, notamment du tissu fixé avec de la formaline et enrobé de paraffine (FFPE), peut être utilisé comme produit de départ.
Les bibliothèques d’ADN qui en résultent sont prêtes pour la préparation d’un modèle en aval pour l’amplification clonale sur les particules Ion Sphere™ à l’aide du système automatisé Ion OneTouch™. Le logiciel Torrent Suite intuitif et le logiciel Ion Reporter en option vous permettent de passer d’ADN extrait à des classifications de variantes en à peine 10 heures.
Library Builder™ Whole Transcriptome Core Kit for 5500 Genetic Analysis Systems (Applied Biosystems™)
Library Builder™ Whole Transcriptome Core Kit for 5500 Genetic Analysis Systems is a reagent component of the AB Library Builder™ System. This kit, along with separately purchased Agencourt® AMPure® Beads, allows for the creation of up to 13 RNA whole transcriptome libraries on the Library Builder™.
• Automation – reduce hands on time by 50% or more depending on the number of libraries
• Scalable – prepare up to 13 libraries per run, up to 26 libraries per day
• Integrated – compatible with the SOLiD® 4 System and 5500 Series Genetic Analysis Systems
Labor Saving Automation
The Library Builder™ Whole Transcriptome Core Kit for 5500 Genetic Analysis Systems when used with the AB Library Builder™ System automates the most labor-intensive parts of RNA whole transcriptome library preparation, helping to reduce hands-on time by at least 50%. The Library Builder™ Whole Transcriptome Core Kit for 5500 Genetic Analysis Systems contains enough reagents and materials to process up to 13 samples at a time.
Integrated RNA Library Preparation
The Library Builder™ Whole Transcriptome Core Kit for 5500 Genetic Analysis Systems, along with the AB Library Builder™ System, is an integrated solution comprising a predefined software protocol, plug-and-play cartridge format reagents, and instrument. These kits are all optimized and validated for RNA purification and RNA whole transcriptome library preparation with the 5500 and SOLiD® 4.
To further maximize automation consider using RNA purification protocols with the iPrep™ PureLink® Total RNA Kit, compatible with the Library Builder™ System.
For Research Use Only. Not for use in diagnostics procedures.
• Automation – reduce hands on time by 50% or more depending on the number of libraries
• Scalable – prepare up to 13 libraries per run, up to 26 libraries per day
• Integrated – compatible with the SOLiD® 4 System and 5500 Series Genetic Analysis Systems
Labor Saving Automation
The Library Builder™ Whole Transcriptome Core Kit for 5500 Genetic Analysis Systems when used with the AB Library Builder™ System automates the most labor-intensive parts of RNA whole transcriptome library preparation, helping to reduce hands-on time by at least 50%. The Library Builder™ Whole Transcriptome Core Kit for 5500 Genetic Analysis Systems contains enough reagents and materials to process up to 13 samples at a time.
Integrated RNA Library Preparation
The Library Builder™ Whole Transcriptome Core Kit for 5500 Genetic Analysis Systems, along with the AB Library Builder™ System, is an integrated solution comprising a predefined software protocol, plug-and-play cartridge format reagents, and instrument. These kits are all optimized and validated for RNA purification and RNA whole transcriptome library preparation with the 5500 and SOLiD® 4.
To further maximize automation consider using RNA purification protocols with the iPrep™ PureLink® Total RNA Kit, compatible with the Library Builder™ System.
For Research Use Only. Not for use in diagnostics procedures.
Ion AmpliSeq™ Exome RDY S5 Kit 1x8 (Ion Torrent™)
The Ion AmpliSeq™ Exome RDY S5 Kit enables a fast and simple method of exome enrichment, with oligo pools for ultra-high multiplex PCR exome enrichment dried down in a 96-well plate. Exome libraries can now be produced with ease in under 6 hours with less than 50 minutes of hands-on time. Also, the Ion AmpliSeq Exome RDY S5 Kit is compatible with the Ion Library Equalizer™ Kit for ease in library normalization. Leveraging the ultra-high multiplex PCR approach of Ion AmpliSeq™ technology, plus template preparation on the Ion Chef™ System and sequencing with the Ion S5™ system, the Ion AmpliSeq Exome RDY S5 Kit allows for rapid sequencing of key exonic regions of the genome, going from DNA to variants in just 2 days.
The Ion AmpliSeq Exome RDY S5 Kit 1x8 configuration includes the following components for 8 exomes (running 2 exomes per Ion 540™ Chip):
• Ion AmpliSeq™ Exome RDY Panel: dried down oligo pools/primers in one 96-well plate with all 8 rows filled (1x8), for ultra-high multiplex PCR enrichment of the exonic regions of the genome
• Ion AmpliSeq™ Library Kit Plus (8 exomes) for robust library preparation of Ion AmpliSeq Exome libraries using Ion AmpliSeq technology
• Ion 540™ Chip Kit for sequencing on the Ion S5 system
The Ion AmpliSeq Exome RDY S5 Kit enables you to:
• Simplify exome enrichment through the combination of dried-down primers and the Ion AmpliSeq workflow, resulting in less than 1 hour of hands-on time to produce exome libraries in under 6 hours
• Accelerate your disease research with rapid exome sequencing, going from DNA to variants in just 2 days
• Access affordable exome sequencing when you need it, without the need to batch dozens of samples or the high price of a lower-throughput run
• Maximize sequencing efficiency with >90% on-target bases and >90% coverage uniformity, and design covering >97% of the Consensus Coding Sequences (CCDS) annotation
• Identify annotated, filtered variants without bioinformatics expertise through fully integrated analysis with Torrent Suite™ and Ion Reporter™ software
Quickest exome enrichment with unprecedented simplicity
The Ion AmpliSeq Exome RDY S5 Kit 1x8 configuration provides a simple method for exome enrichment. The same primers used in the Ion AmpliSeq™ Exome Kit 4xDuo and Ion AmpliSeq™ Exome Kit 8xTrio have been dried down into one 96-well plate, using the 12 wells of a single row for each of the 12 primer pools, and with 8 rows in total, leading to the use of one row per exome. The kit is sufficient to prepare 8 exome libraries. Additionally, the dried down primers contain a light blue dye for easy identification of used or occupied rows. With a workflow requiring less than 50 minutes of hands-on time and no centrifugation steps, the kit enables exome library preparation in less than 6 hours. Additionally, the Ion AmpliSeq Exome RDY S5 Kit maintains a low input requirement of 50 ng DNA. With the included Ion AmpliSeq Library Kit Plus, exome library preparation is more robust, resulting in higher uniformity and consistently higher library yield when performing library amplification. Also, the Ion AmpliSeq Exome RDY S5 Kit is now compatible with the Ion Library Equalizer Kit for ultimate ease in library normalization.
Lowest cost per exome using a benchtop sequencer
Paired with the Ion S5 system, the Ion AmpliSeq Exome RDY S5 Kit allows disease researchers to access affordable exome sequencing on their benchtops without long turn-around times or the sample batching normally required to achieve a low cost per sample. Using the Ion AmpliSeq Exome RDY S5 Kit with the Ion S5 system enables you to go from DNA to variants in as little as 2 days. Additionally, with uniform exome enrichment covering >97% of the CCDS, you can achieve efficient sequencing with 2 exomes run per Ion 540™ chip.
Fully integrated analysis without bioinformatics expertise
Use of the kit with Torrent Suite and Ion Reporter software provides a simple and streamlined analysis workflow that automates the identification of your variants of interest without needing the expertise or infrastructure of a full bioinformatics team. Additionally, the Ion S5 sequencer along with Ion Reporter Software deliver both SNP and indel information along with copy number changes through a single analysis workflow, advancing your human disease research.
Note: This kit is compatible with the Ion Chef™, Ion OneTouch™ 2, and Ion S5™ systems.
The Ion AmpliSeq Exome RDY S5 Kit 1x8 configuration includes the following components for 8 exomes (running 2 exomes per Ion 540™ Chip):
• Ion AmpliSeq™ Exome RDY Panel: dried down oligo pools/primers in one 96-well plate with all 8 rows filled (1x8), for ultra-high multiplex PCR enrichment of the exonic regions of the genome
• Ion AmpliSeq™ Library Kit Plus (8 exomes) for robust library preparation of Ion AmpliSeq Exome libraries using Ion AmpliSeq technology
• Ion 540™ Chip Kit for sequencing on the Ion S5 system
The Ion AmpliSeq Exome RDY S5 Kit enables you to:
• Simplify exome enrichment through the combination of dried-down primers and the Ion AmpliSeq workflow, resulting in less than 1 hour of hands-on time to produce exome libraries in under 6 hours
• Accelerate your disease research with rapid exome sequencing, going from DNA to variants in just 2 days
• Access affordable exome sequencing when you need it, without the need to batch dozens of samples or the high price of a lower-throughput run
• Maximize sequencing efficiency with >90% on-target bases and >90% coverage uniformity, and design covering >97% of the Consensus Coding Sequences (CCDS) annotation
• Identify annotated, filtered variants without bioinformatics expertise through fully integrated analysis with Torrent Suite™ and Ion Reporter™ software
Quickest exome enrichment with unprecedented simplicity
The Ion AmpliSeq Exome RDY S5 Kit 1x8 configuration provides a simple method for exome enrichment. The same primers used in the Ion AmpliSeq™ Exome Kit 4xDuo and Ion AmpliSeq™ Exome Kit 8xTrio have been dried down into one 96-well plate, using the 12 wells of a single row for each of the 12 primer pools, and with 8 rows in total, leading to the use of one row per exome. The kit is sufficient to prepare 8 exome libraries. Additionally, the dried down primers contain a light blue dye for easy identification of used or occupied rows. With a workflow requiring less than 50 minutes of hands-on time and no centrifugation steps, the kit enables exome library preparation in less than 6 hours. Additionally, the Ion AmpliSeq Exome RDY S5 Kit maintains a low input requirement of 50 ng DNA. With the included Ion AmpliSeq Library Kit Plus, exome library preparation is more robust, resulting in higher uniformity and consistently higher library yield when performing library amplification. Also, the Ion AmpliSeq Exome RDY S5 Kit is now compatible with the Ion Library Equalizer Kit for ultimate ease in library normalization.
Lowest cost per exome using a benchtop sequencer
Paired with the Ion S5 system, the Ion AmpliSeq Exome RDY S5 Kit allows disease researchers to access affordable exome sequencing on their benchtops without long turn-around times or the sample batching normally required to achieve a low cost per sample. Using the Ion AmpliSeq Exome RDY S5 Kit with the Ion S5 system enables you to go from DNA to variants in as little as 2 days. Additionally, with uniform exome enrichment covering >97% of the CCDS, you can achieve efficient sequencing with 2 exomes run per Ion 540™ chip.
Fully integrated analysis without bioinformatics expertise
Use of the kit with Torrent Suite and Ion Reporter software provides a simple and streamlined analysis workflow that automates the identification of your variants of interest without needing the expertise or infrastructure of a full bioinformatics team. Additionally, the Ion S5 sequencer along with Ion Reporter Software deliver both SNP and indel information along with copy number changes through a single analysis workflow, advancing your human disease research.
Note: This kit is compatible with the Ion Chef™, Ion OneTouch™ 2, and Ion S5™ systems.
SOLiD™ RNA Barcoding Kit, Module 1-48 (Applied Biosystems™)
The SOLiD™ RNA Barcoding Kit consists of a set of PCR primers that are designed for use with the SOLiD™ Total RNA-Seq Kit or the SOLiD™ SAGE™ Kit with Barcoding Adaptor Module. Together, these combinations of kits enable multiplex sequencing for all RNA applications, including whole transcriptome, small RNA analysis, or SOLiD™ SAGE™.
Key Product Features:
• Cost-effective runs—barcodes enable sequencing of multiple libraries at once
• Modular configuration—each module contains 16 unique barcodes
Increase the Power of Your Studies
Barcoding enables the assignment of a unique identifier to templated beads made from a single library. Once the identifiers are assigned, multiple batches of templated beads may be pooled together for emulsion PCR and then sequenced. The SOLiD™ RNA Barcoding Kit increases the power of your studies by enabling cost effective sequencing of multiple libraries on the same slide—maximizing throughput while dramatically reducing costs. (Figure 1).
Modules of 16 barcodes for RNA Sequencing
The SOLiD™ RNA Barcoding Kit Modules each consist of a set of 16 PCR primers with unique barcodes. There is sufficient material to label 3 libraries with each of the 16 barcodes in each module (96 reactions). The barcodes are selected for uniform melting temperature, low error rate, and orthogonal sequences that are unique in color space.
• The SOLiD™ 5' PCR Primer is identical to the 5’ PCR primer provided in the SOLiD™ Total RNA-Seq Kit and it contains the SOLiD™ emulsion PCR primer P1 sequence.
• The SOLiD™ 3' (reverse) PCR primers contain the P2 sequence required for SOLiD™ emulsion PCR, along with a unique barcode sequence, and an internal adaptor (IA) sequence necessary for sequencing the barcode (Figure 2).
Using these primers in the cDNA amplification step of the procedure generates barcoded cDNA libraries that can be pooled for emulsion PCR and SOLiD™ System sequencing. Additional rounds of ligation-based sequencing are performed using primer sets complimentary to the barcode. The resulting reads can then be sorted by the barcode and aligned in groups to the reference sequence.
Key Product Features:
• Cost-effective runs—barcodes enable sequencing of multiple libraries at once
• Modular configuration—each module contains 16 unique barcodes
Increase the Power of Your Studies
Barcoding enables the assignment of a unique identifier to templated beads made from a single library. Once the identifiers are assigned, multiple batches of templated beads may be pooled together for emulsion PCR and then sequenced. The SOLiD™ RNA Barcoding Kit increases the power of your studies by enabling cost effective sequencing of multiple libraries on the same slide—maximizing throughput while dramatically reducing costs. (Figure 1).
Modules of 16 barcodes for RNA Sequencing
The SOLiD™ RNA Barcoding Kit Modules each consist of a set of 16 PCR primers with unique barcodes. There is sufficient material to label 3 libraries with each of the 16 barcodes in each module (96 reactions). The barcodes are selected for uniform melting temperature, low error rate, and orthogonal sequences that are unique in color space.
• The SOLiD™ 5' PCR Primer is identical to the 5’ PCR primer provided in the SOLiD™ Total RNA-Seq Kit and it contains the SOLiD™ emulsion PCR primer P1 sequence.
• The SOLiD™ 3' (reverse) PCR primers contain the P2 sequence required for SOLiD™ emulsion PCR, along with a unique barcode sequence, and an internal adaptor (IA) sequence necessary for sequencing the barcode (Figure 2).
Using these primers in the cDNA amplification step of the procedure generates barcoded cDNA libraries that can be pooled for emulsion PCR and SOLiD™ System sequencing. Additional rounds of ligation-based sequencing are performed using primer sets complimentary to the barcode. The resulting reads can then be sorted by the barcode and aligned in groups to the reference sequence.
Ion Xpress™ Barcode Adapters 65-80 Kit (Ion Torrent™)
The Ion Xpress™ Barcode Adapters 65-80 Kit provides a set of 16 unique barcode adaptors specifically designed and validated for optimal performance with the Ion Torrent PGM™ and Proton™ semiconductor sequencers. When used in combination with the Ion Xpress™ Plus Fragment Library Kit or the Ion Plus Fragment Library Kit, this kit enables users to pool up to 16 fragment libraries prior to emulsion PCR and then conduct multiplexed sequencing analysis, simplifying the Ion semiconductor sequencing workflow for a wide range of applications, including targeted enrichment. Use of this kit with other Barcode Adapters kits allows pooling of up to 96 fragment libraries.
Key Product Features:
• Enables multiplexing of numerous fragment library samples on a single sequencing chip with the use of robust molecular barcodes
• Both sequence- and flow-optimized for equal representation all barcodes in a pool and more economical multiplexed sequencing runs
• Robust error correction built in by design for added confidence in sample identification
Multiplexing with Barcode Adaptors Enables Higher Throughput
Multiplexing with barcoded libraries leads to more cost effective runs by permitting up to 96 samples per run (16 with this kit), significantly decreasing the cost and handling requirements of emPCR, enrichment, and sequencing.
Sequence- and Flow-Optimized for Increased Performance and Efficiency
Barcode adapters contain sequences that are optimized to provide equal representation of all barcodes in a pool, require a minimum number of flows to interrogate, and require only 17 bases of adaptor sequencing required which results in more economical multiplexed sequencing runs.
Robust Error Correction Built In by Design
Built-in error correction virtually eliminates the possibility of incorrect sample assignment.
For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
Key Product Features:
• Enables multiplexing of numerous fragment library samples on a single sequencing chip with the use of robust molecular barcodes
• Both sequence- and flow-optimized for equal representation all barcodes in a pool and more economical multiplexed sequencing runs
• Robust error correction built in by design for added confidence in sample identification
Multiplexing with Barcode Adaptors Enables Higher Throughput
Multiplexing with barcoded libraries leads to more cost effective runs by permitting up to 96 samples per run (16 with this kit), significantly decreasing the cost and handling requirements of emPCR, enrichment, and sequencing.
Sequence- and Flow-Optimized for Increased Performance and Efficiency
Barcode adapters contain sequences that are optimized to provide equal representation of all barcodes in a pool, require a minimum number of flows to interrogate, and require only 17 bases of adaptor sequencing required which results in more economical multiplexed sequencing runs.
Robust Error Correction Built In by Design
Built-in error correction virtually eliminates the possibility of incorrect sample assignment.
For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
5500 SOLiD™ Fragment Library Barcode Adaptors 1-16 (Applied Biosystems™)
The 5500 SOLiD™ Fragment Library Barcode Adaptor Kit provides a complete set of 96 unique barcode adaptors and primers for cost-effective next generation sequencing of fragment libraries on the 5500 Series Genetic Analysis Systems. When used in combination with the 5500 SOLiD™ Fragment Library Core Kit, the 5500 SOLiD™ Fragment Library Barcoding Kit enables users to pool up to 96 fragment libraries prior to emulsion PCR and then conduct multiplexed sequencing analysis, simplifying the next generation sequencing workflow for a wide range of applications, including targeted enrichment
Key Product Features:
• Efficient multiplexing—enables multiplexing of up to 96 fragment or paired-end library samples on a single flow chip with the use of robust molecular barcodes.
• Cost effective runs—allows more samples per run, significantly decreasing the cost and handling requirements of ePCR, enrichment, and deposition.
• Consolidated 5500 SOLiD sampling—enables pooling of samples prior to emulsion PCR (ePCR) and sequencing on the 5500 Series Genetic Analysis Systems.
• Versatile sequencing—substantially benefits applications such as whole genome sequencing of small genomes or targeted resequencing of enriched samples.
For Research Use Only. Not for use in diagnostics procedures.
Key Product Features:
• Efficient multiplexing—enables multiplexing of up to 96 fragment or paired-end library samples on a single flow chip with the use of robust molecular barcodes.
• Cost effective runs—allows more samples per run, significantly decreasing the cost and handling requirements of ePCR, enrichment, and deposition.
• Consolidated 5500 SOLiD sampling—enables pooling of samples prior to emulsion PCR (ePCR) and sequencing on the 5500 Series Genetic Analysis Systems.
• Versatile sequencing—substantially benefits applications such as whole genome sequencing of small genomes or targeted resequencing of enriched samples.
For Research Use Only. Not for use in diagnostics procedures.
