Shop All Sequencing Kits
SOLiD™ FWD SR S75 Kit, Double (Applied Biosystems™)
SOLiD® Sequencing Reagents (SR) Kits contain enzymes and probes needed for ligation sequencing with 2-base encoded (2BE) probes on the 5500/5500xl Genetic Analyzer. The SOLiD® FWD SR Kits are used for fragment, mate-paired, and paired-end sequencing runs, for ligation sequencing in the forward direction.
Double-sized kits have sufficient reagents for covering two FlowChips.
The SOLiD® FWD SR S75 Kit, Double contains sufficient ligase, phosphatase, and forward 2BE probes to run 750 to 1032 cycles, or up to 86 cycles per lane for 12 lanes. The kit is best configured for sequencing read lengths up to 75 bp for barcoded runs. In addition to the SOLiD® Run Cycle Buffers and SOLiD® Buffer D (Reset), this kit requires the use of the SOLiD® FWD Buffer. Store the SOLiD® FWD SR S75 Kits at –25°C to –15°C until ready for use.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Double-sized kits have sufficient reagents for covering two FlowChips.
The SOLiD® FWD SR S75 Kit, Double contains sufficient ligase, phosphatase, and forward 2BE probes to run 750 to 1032 cycles, or up to 86 cycles per lane for 12 lanes. The kit is best configured for sequencing read lengths up to 75 bp for barcoded runs. In addition to the SOLiD® Run Cycle Buffers and SOLiD® Buffer D (Reset), this kit requires the use of the SOLiD® FWD Buffer. Store the SOLiD® FWD SR S75 Kits at –25°C to –15°C until ready for use.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Ion PGM™ Hi-Q™ Chef Kit (Ion Torrent™)
Le kit Ion PGM™ Hi-Q™ Chef permet la préparation de modèles précis et reproductibles, le chargement de puces et le séquençage de bibliothèques de 400 bp (avec achat supplémentaire*) et 200 bp sur le système Ion Chef™. Il est compatible avec le système Ion PGM™. Les cartouches de réactif prêtes à l’emploi, pré-emballées et à usage unique permettent un flux de travaux complètement automatisé en traitant deux échantillons par cycle pour une productivité accrue. Les code-barres du réactif et de l’échantillon contribuent à vérifier le plan d’analyse défini par l’utilisateur et permettent la traçabilité complète de l’analyse.
• Permet la préparation automatisée des modèles et le chargement des puces pour les bibliothèques de 400 et 200 paires de base
• Fournit la chimie de séquençage la plus précise qui soit pour le système Ion PGM™
• Conçu pour minimiser le temps de manipulation nécessaire pour préparer vos échantillons
• Aide à augmenter le débit du laboratoire grâce au traitement de deux échantillons
Maximisez vos résultats avec une chimie de séquençage à haute précision
Le kit Ion PGM™ Hi-Q™ Chef est un composant intégral du flux de travaux du séquençage de nouvelle génération Ion Torrent™. Conçu pour simplifier le flux de travaux global avec un temps de manipulation minimal, le système Ion Chef™ est idéal pour les utilisateurs ayant toutes sortes de niveaux d’expérience. Le kit Ion PGM™ Hi-Q™ Chef est doté des chimies de modélisation et de séquençage les plus avancées à la disposition des utilisateurs de l’Ion PGM™. Utilisez le kit de séquençage Ion PGM™ Hi-Q™ pour aider à maximiser vos performances de détection de variantes (“variant calling”) et améliorer les applications de séquençage de novo.
Un seul kit pour répondre à tous vos besoins de séquençage Ion PGM™
Le kit Ion PGM™ Hi-Q™ Chef est configuré pour répondre à tous vos besoins de séquençage concernant les applications nécessitant des longueurs de lecture de 200 ou 400 bp*. Ce kit prend en charge Ion 314™ Chip v2 BC, Ion 316™ Chip v2 BC et Ion 318™ Chip v2 BC. Le kit contient suffisamment de réactifs pour huit réactions de séquençage de 200 bp.
*Pour les applications de 400 bases, commandez le kit 400 de fournitures Ion PGM™ Hi-Q™ Chef, qui fournit les réactifs supplémentaires nécessaires pour huit réactions de séquençage de 400 bp.
• Permet la préparation automatisée des modèles et le chargement des puces pour les bibliothèques de 400 et 200 paires de base
• Fournit la chimie de séquençage la plus précise qui soit pour le système Ion PGM™
• Conçu pour minimiser le temps de manipulation nécessaire pour préparer vos échantillons
• Aide à augmenter le débit du laboratoire grâce au traitement de deux échantillons
Maximisez vos résultats avec une chimie de séquençage à haute précision
Le kit Ion PGM™ Hi-Q™ Chef est un composant intégral du flux de travaux du séquençage de nouvelle génération Ion Torrent™. Conçu pour simplifier le flux de travaux global avec un temps de manipulation minimal, le système Ion Chef™ est idéal pour les utilisateurs ayant toutes sortes de niveaux d’expérience. Le kit Ion PGM™ Hi-Q™ Chef est doté des chimies de modélisation et de séquençage les plus avancées à la disposition des utilisateurs de l’Ion PGM™. Utilisez le kit de séquençage Ion PGM™ Hi-Q™ pour aider à maximiser vos performances de détection de variantes (“variant calling”) et améliorer les applications de séquençage de novo.
Un seul kit pour répondre à tous vos besoins de séquençage Ion PGM™
Le kit Ion PGM™ Hi-Q™ Chef est configuré pour répondre à tous vos besoins de séquençage concernant les applications nécessitant des longueurs de lecture de 200 ou 400 bp*. Ce kit prend en charge Ion 314™ Chip v2 BC, Ion 316™ Chip v2 BC et Ion 318™ Chip v2 BC. Le kit contient suffisamment de réactifs pour huit réactions de séquençage de 200 bp.
*Pour les applications de 400 bases, commandez le kit 400 de fournitures Ion PGM™ Hi-Q™ Chef, qui fournit les réactifs supplémentaires nécessaires pour huit réactions de séquençage de 400 bp.
Ion ReproSeq™ PGS 316 View Kit, without chips (Ion Torrent™)
The Ion ReproSeq™ PGS View kits enable a rapid benchtop next-generation sequencing (NGS) workflow for aneuploidy analysis. When used with the Ion PGM™ System, they allow for rapid and affordable detection of aneuploidies in all 24 sets of chromosomes, for labs interested in rapidly and reliably assessing pre-implantation embryos for research. Using as little as 6 pg of DNA from a single cell, Ion ReproSeq PGS View kits enable accurate detection of whole chromosome and chromosome arm events (≥48 Mb) in as little as 8 hours.* The Ion PGM sequencing system provides throughput flexibility, enabling the cost-effective screening of 2 to 24 samples and minimizing the need to batch samples.
*Turnaround time for the Ion ReproSeq PGS View workflow is as little as 8 hours with the Ion 314™ Chip and as little as 10 hours with the Ion 318™ Chip.
Cost-effective analysis workflow with sample throughput flexibility
The Ion ReproSeq PGS View kits and the Ion PGM System deliver scalability and flexibility for various levels of sample throughput. Choose the one that fits your throughput needs from a selection of 3 sequencing chips for the processing of 2 to 24 samples, thereby minimizing the need for batching to achieve cost efficiency.
Rapid turnaround for time-sensitive samples
Leveraging the speed of semiconductor sequencing and template preparation by isothermal amplification, the Ion ReproSeq PGS View kits offer a fast benchtop NGS workflow for aneuploidy analysis. Go from single cells isolated from a pre-implantation embryo to aneuploidy detection in as little as 8 hours when screening up to 2 samples or as little as 10 hours with up to 24 samples. This rapid turnaround time enables fast identification of chromosomally normal embryos for research.
The Ion ReproSeq™ PGS 316 View Kit, without chips, includes all reagents for whole genome amplification, library construction, template preparation, and sequencing using the Ion PGM System for processing a total of [24/120/192] samples. The kit contains:
• Ion SingleSeq™ Kit (24 reactions/kit)—allows for the extraction, amplification, and barcoding of genomic DNA, with completion in less than 4 hours (including hands-on time)
• Ion PGM™ Template IA 500 Kit (A24622, 8 reactions/kit)—enables the fastest template preparation (2 hours, including hands-on time) whereby DNA is clonally amplified onto a bead surface through a non-emulsion, isothermal reaction
• Ion PGM™ Hi-Q™ View Sequencing Kit (A30044, 8 reactions/kit at 200 bp)—offers our most advanced sequencing chemistry and is configured to support all 200 bp sequencing needs
• Ion PGM™ Wash 2 Bottle Kit (A25591)—contains conditioning solution and a Wash 2 bottle that may be used for up to 40 runs before replacement
Notes
• An Ion OneTouch™ ES (enrichment system), Ion PGM™ Enrichment Beads (4478525), and Ion 316™ Chip Kit v2 BC (4488149) are required for use with this sequencing kit.
• This kit is only compatible with the Ion PGM System.
*Turnaround time for the Ion ReproSeq PGS View workflow is as little as 8 hours with the Ion 314™ Chip and as little as 10 hours with the Ion 318™ Chip.
Cost-effective analysis workflow with sample throughput flexibility
The Ion ReproSeq PGS View kits and the Ion PGM System deliver scalability and flexibility for various levels of sample throughput. Choose the one that fits your throughput needs from a selection of 3 sequencing chips for the processing of 2 to 24 samples, thereby minimizing the need for batching to achieve cost efficiency.
Rapid turnaround for time-sensitive samples
Leveraging the speed of semiconductor sequencing and template preparation by isothermal amplification, the Ion ReproSeq PGS View kits offer a fast benchtop NGS workflow for aneuploidy analysis. Go from single cells isolated from a pre-implantation embryo to aneuploidy detection in as little as 8 hours when screening up to 2 samples or as little as 10 hours with up to 24 samples. This rapid turnaround time enables fast identification of chromosomally normal embryos for research.
The Ion ReproSeq™ PGS 316 View Kit, without chips, includes all reagents for whole genome amplification, library construction, template preparation, and sequencing using the Ion PGM System for processing a total of [24/120/192] samples. The kit contains:
• Ion SingleSeq™ Kit (24 reactions/kit)—allows for the extraction, amplification, and barcoding of genomic DNA, with completion in less than 4 hours (including hands-on time)
• Ion PGM™ Template IA 500 Kit (A24622, 8 reactions/kit)—enables the fastest template preparation (2 hours, including hands-on time) whereby DNA is clonally amplified onto a bead surface through a non-emulsion, isothermal reaction
• Ion PGM™ Hi-Q™ View Sequencing Kit (A30044, 8 reactions/kit at 200 bp)—offers our most advanced sequencing chemistry and is configured to support all 200 bp sequencing needs
• Ion PGM™ Wash 2 Bottle Kit (A25591)—contains conditioning solution and a Wash 2 bottle that may be used for up to 40 runs before replacement
Notes
• An Ion OneTouch™ ES (enrichment system), Ion PGM™ Enrichment Beads (4478525), and Ion 316™ Chip Kit v2 BC (4488149) are required for use with this sequencing kit.
• This kit is only compatible with the Ion PGM System.
5500 W Template Amplification Kit v2 (Applied Biosystems™)
The 5500 W Template Amplification Kit v2 contains reagents needed for amplifying library template onto the 5500 W FlowChip for sequencing on the 5500 W Series genetic analyzers. The kit is comprised of a strip tube that holds the enzymes, dNTPs, and buffers, allowing automated template amplification on the 5500 W Series genetic analyzers. The kit is used for fragment, mate-paired, and paired-end sequencing runs. This kit contains sufficient reagents to run 6 lanes.
dGTP BigDye™ Terminator v3.0 Ready Reaction Cycle Sequencing Kit (Applied Biosystems™)
The dGTP BigDye® Terminator Cycle Sequencing Kit's fluorescent dideoxy terminator chemistry is optimized for sequencing through GT- and G-rich templates.
• Ensure reliable results with difficult regions and finishing applications.
• Sequence templates with challenging sequence contexts.
• Sequence problematic templates that standard chemistries cannot.
Sequence Challenging Templates
The dGTP BigDye® Terminator Kit is ideal for working with GT- and G-rich and other difficult-to-sequence templates. The dGTP BigDye® Terminator Kit replaces dITP with dGTP and it enables you to extend through those difficult-to-sequence regions with confidence, avoiding early signal loss in these samples.
Optimized for GT-Rich Templates
Obtain higher success rate even with your difficult GT-rich templates. By using the same chemistries as the standard BigDye® Terminator Kits (only replacing dITP with dGTP) the dGTP BigDye® Terminator Kit provides proven performance, optimized for GT-rich and other difficult-to-sequence templates.
For Research Use Only. Not for use in diagnostics procedures.
• Ensure reliable results with difficult regions and finishing applications.
• Sequence templates with challenging sequence contexts.
• Sequence problematic templates that standard chemistries cannot.
Sequence Challenging Templates
The dGTP BigDye® Terminator Kit is ideal for working with GT- and G-rich and other difficult-to-sequence templates. The dGTP BigDye® Terminator Kit replaces dITP with dGTP and it enables you to extend through those difficult-to-sequence regions with confidence, avoiding early signal loss in these samples.
Optimized for GT-Rich Templates
Obtain higher success rate even with your difficult GT-rich templates. By using the same chemistries as the standard BigDye® Terminator Kits (only replacing dITP with dGTP) the dGTP BigDye® Terminator Kit provides proven performance, optimized for GT-rich and other difficult-to-sequence templates.
For Research Use Only. Not for use in diagnostics procedures.
Ion ReproSeq™ PGS 318 View Kit, without chips (Ion Torrent™)
The Ion ReproSeq™ PGS View kits enable a rapid benchtop next-generation sequencing (NGS) workflow for aneuploidy analysis. When used with the Ion PGM™ System, they allow for rapid and affordable detection of aneuploidies in all 24 sets of chromosomes, for labs interested in rapidly and reliably assessing pre-implantation embryos for research. Using as little as 6 pg of DNA from a single cell, Ion ReproSeq PGS View kits enable accurate detection of whole chromosome and chromosome arm events (≥48 Mb) in as little as 8 hours.* The Ion PGM sequencing system provides throughput flexibility, enabling the cost-effective screening of 2 to 24 samples and minimizing the need to batch samples.
*Turnaround time for the Ion ReproSeq PGS View workflow is as little as 8 hours with the Ion 314™ Chip and as little as 10 hours with the Ion 318™ Chip.
Cost-effective analysis workflow with sample throughput flexibility
The Ion ReproSeq PGS View kits and the Ion PGM System deliver scalability and flexibility for various levels of sample throughput. Choose the one that fits your throughput needs from a selection of 3 sequencing chips for the processing of 2 to 24 samples, thereby minimizing the need for batching to achieve cost efficiency.
Rapid turnaround for time-sensitive samples
Leveraging the speed of semiconductor sequencing and template preparation by isothermal amplification, the Ion ReproSeq PGS View kits offer a fast benchtop NGS workflow for aneuploidy analysis. Go from single cells isolated from a pre-implantation embryo to aneuploidy detection in as little as 8 hours when screening up to 2 samples or as little as 10 hours with up to 24 samples. This rapid turnaround time enables fast identification of chromosomally normal embryos for research.
The Ion ReproSeq™ PGS 318 View Kit, without chips, includes all reagents for whole genome amplification, library construction, template preparation, and sequencing using the Ion PGM System for processing a total of 192 samples. The kit contains:
• Ion SingleSeq™ Kit (24 reactions/kit)—allows for the extraction, amplification, and barcoding of genomic DNA, with completion in less than 4 hours (including hands-on time)
• Ion PGM™ Template IA 500 Kit (A24622, 8 reactions/kit)—enables the fastest template preparation (2 hours, including hands-on time) whereby DNA is clonally amplified onto a bead surface through a non-emulsion, isothermal reaction
• Ion PGM™ Hi-Q™ View Sequencing Kit (A30044, 8 reactions/kit at 200 bp)—offers our most advanced sequencing chemistry and is configured to support all 200 bp sequencing needs
• Ion PGM™ Wash 2 Bottle Kit (A25591)—contains conditioning solution and a Wash 2 bottle that may be used for up to 40 runs before replacement
Notes
• An Ion OneTouch™ ES (enrichment system), Ion PGM™ Enrichment Beads (4478525), and Ion 318™ Chip Kit v2 BC (4488150) are required for use with this sequencing kit.
• This kit is only compatible with the Ion PGM System.
*Turnaround time for the Ion ReproSeq PGS View workflow is as little as 8 hours with the Ion 314™ Chip and as little as 10 hours with the Ion 318™ Chip.
Cost-effective analysis workflow with sample throughput flexibility
The Ion ReproSeq PGS View kits and the Ion PGM System deliver scalability and flexibility for various levels of sample throughput. Choose the one that fits your throughput needs from a selection of 3 sequencing chips for the processing of 2 to 24 samples, thereby minimizing the need for batching to achieve cost efficiency.
Rapid turnaround for time-sensitive samples
Leveraging the speed of semiconductor sequencing and template preparation by isothermal amplification, the Ion ReproSeq PGS View kits offer a fast benchtop NGS workflow for aneuploidy analysis. Go from single cells isolated from a pre-implantation embryo to aneuploidy detection in as little as 8 hours when screening up to 2 samples or as little as 10 hours with up to 24 samples. This rapid turnaround time enables fast identification of chromosomally normal embryos for research.
The Ion ReproSeq™ PGS 318 View Kit, without chips, includes all reagents for whole genome amplification, library construction, template preparation, and sequencing using the Ion PGM System for processing a total of 192 samples. The kit contains:
• Ion SingleSeq™ Kit (24 reactions/kit)—allows for the extraction, amplification, and barcoding of genomic DNA, with completion in less than 4 hours (including hands-on time)
• Ion PGM™ Template IA 500 Kit (A24622, 8 reactions/kit)—enables the fastest template preparation (2 hours, including hands-on time) whereby DNA is clonally amplified onto a bead surface through a non-emulsion, isothermal reaction
• Ion PGM™ Hi-Q™ View Sequencing Kit (A30044, 8 reactions/kit at 200 bp)—offers our most advanced sequencing chemistry and is configured to support all 200 bp sequencing needs
• Ion PGM™ Wash 2 Bottle Kit (A25591)—contains conditioning solution and a Wash 2 bottle that may be used for up to 40 runs before replacement
Notes
• An Ion OneTouch™ ES (enrichment system), Ion PGM™ Enrichment Beads (4478525), and Ion 318™ Chip Kit v2 BC (4488150) are required for use with this sequencing kit.
• This kit is only compatible with the Ion PGM System.
5500 W FWD SR75 Kit (Applied Biosystems™)
5500 W FWD Sequencing Reagent (SR) kits contain the phosphatase and probes needed for ligation sequencing with 2-base encoded (2BE) probes on the 5500 W series genetic analyzers. The 5500 W FWD SR75 Kit consists of an 8-well striptube that contains sufficient phosphatase and probes to run up to 510 cycles and is best configured for sequencing read-lengths up to 75 bases with a 10-base barcode for 6 lanes.
Ion PGM™ Wash 2 Bottle Kit (Ion Torrent™)
Le kit de flacon de lavage 2 Ion PGM™ Hi-Q™ est utilisé conjointement avec le kit de séquençage Ion PGM™ Hi-Q™. Le kit de flacon contient une solution de conditionnement et un flacon de lavage 2 qui peut être utilisé pour un maximum de 40 cycles avant remplacement. La possibilité de réutilisation de ce flacon de lavage permet non seulement une meilleure reproductibilité d’un cycle à l’autre mais également moins de déchets et des économies à chaque cycle.
Ion ReproSeq™ PGS 316 Kit, with chips (Ion Torrent™)
The Ion ReproSeq™ PGS kits enable the fastest benchtop next-generation sequencing workflow for aneuploidy analysis. The Ion ReproSeq PGS Kit with the Ion PGM™ System allows for rapid and affordable detection of aneuploidies in all 24 sets of chromosomes for labs interested in rapidly and reliably assessing preimplantation embryos. Using as little as 6 pg of DNA from a single cell, this kit enables accurate detection of whole chromosome and chromosome arm events (≥48 Mb) in as little as 8 hours.* The Ion PGM System provides throughput flexibility, enabling the cost-effective screening of 2 to 24 samples and minimizing the need to batch samples.
*Turnaround time for the Ion ReproSeq PGS workflow is as little as 8 hours with the Ion 314™ Chip and as little as 10 hours with the Ion 318™ Chip.
Cost-effective analysis workflow with sample throughput flexibility
The Ion ReproSeq PGS Kit and the Ion PGM System deliver scalability and flexibility for various levels of sample throughput. Choose the one that fits your throughput needs from a range of 3 sequencing chips to process 2 to 24 samples and minimize the need for batching to achieve cost efficiency.
Rapid turnaround for time-sensitive samples
Leveraging the speed of semiconductor sequencing and template preparation by isothermal amplification, the Ion ReproSeq PGS Kit offers a fast benchtop NGS workflow for aneuploidy analysis. Go from single cells isolated from a preimplantion embryo to aneuploidy detection in as little as 8 hours when screening up to 2 samples or as little as 10 hours with up to 24 samples. This rapid turnaround time enables fast identification of chromosomally normal embryos.
The Ion ReproSeq PGS 316 Kit includes all materials for whole genome amplification, library construction, template preparation, and sequencing using the Ion PGM System for processing a total of 120 samples. The kit contains:
• Ion SingleSeq™ Kit (A25589, 24 reactions/kit)—allows for the extraction, amplification, and addition of barcoding of genomic DNA, a process that can be completed in less than 4 hours (includes hands on time)
• Ion PGM™ Template IA 500 Kit (A24622, 8 reactions/kit)—the fastest template preparation (2 hours, includes hands on time) whereby DNA is clonally amplified onto a bead surface through a non-emulsion, isothermal reaction
• Ion PGM™ Hi-Q™ Sequencing Kit (A25592, 8 reactions/kit at 200 bp)—offers the most advanced sequencing chemistry available and is configured to support all of your sequencing needs for applications requiring 200 bp
• Ion 316™ Chip Kit v2 BC (4488149)—contains 8 barcoded chips per kit for sample tracking and sequencing runs using the Ion PGM System
• Ion PGM™ Wash 2 Bottle Kit (A25591)—contains conditioning solution and a Wash 2 bottle that may be used for up to 40 runs before replacement
Notes
• An Ion OneTouch™ ES (enrichment system) and Ion PGM™ Enrichment Beads (4478525) are required for use with this sequencing kit.
• This kit is only compatible with the Ion PGM System.
*Turnaround time for the Ion ReproSeq PGS workflow is as little as 8 hours with the Ion 314™ Chip and as little as 10 hours with the Ion 318™ Chip.
Cost-effective analysis workflow with sample throughput flexibility
The Ion ReproSeq PGS Kit and the Ion PGM System deliver scalability and flexibility for various levels of sample throughput. Choose the one that fits your throughput needs from a range of 3 sequencing chips to process 2 to 24 samples and minimize the need for batching to achieve cost efficiency.
Rapid turnaround for time-sensitive samples
Leveraging the speed of semiconductor sequencing and template preparation by isothermal amplification, the Ion ReproSeq PGS Kit offers a fast benchtop NGS workflow for aneuploidy analysis. Go from single cells isolated from a preimplantion embryo to aneuploidy detection in as little as 8 hours when screening up to 2 samples or as little as 10 hours with up to 24 samples. This rapid turnaround time enables fast identification of chromosomally normal embryos.
The Ion ReproSeq PGS 316 Kit includes all materials for whole genome amplification, library construction, template preparation, and sequencing using the Ion PGM System for processing a total of 120 samples. The kit contains:
• Ion SingleSeq™ Kit (A25589, 24 reactions/kit)—allows for the extraction, amplification, and addition of barcoding of genomic DNA, a process that can be completed in less than 4 hours (includes hands on time)
• Ion PGM™ Template IA 500 Kit (A24622, 8 reactions/kit)—the fastest template preparation (2 hours, includes hands on time) whereby DNA is clonally amplified onto a bead surface through a non-emulsion, isothermal reaction
• Ion PGM™ Hi-Q™ Sequencing Kit (A25592, 8 reactions/kit at 200 bp)—offers the most advanced sequencing chemistry available and is configured to support all of your sequencing needs for applications requiring 200 bp
• Ion 316™ Chip Kit v2 BC (4488149)—contains 8 barcoded chips per kit for sample tracking and sequencing runs using the Ion PGM System
• Ion PGM™ Wash 2 Bottle Kit (A25591)—contains conditioning solution and a Wash 2 bottle that may be used for up to 40 runs before replacement
Notes
• An Ion OneTouch™ ES (enrichment system) and Ion PGM™ Enrichment Beads (4478525) are required for use with this sequencing kit.
• This kit is only compatible with the Ion PGM System.
Ion PI™ Hi-Q™ Sequencing 200 Kit (2 sequencing runs per initialization) (Ion Torrent™)
The Ion PI™ Hi-Q™ Sequencing 200 Kit contains the most advanced reagents for sequencing using the Ion Proton™ System. The kit contains a novel sequencing enzyme that enables increased variant calling accuracy. When used together with the Ion PI™ Hi-Q™ OT2 200 Kit, the Ion PI™ Hi-Q™ Sequencing 200 Kit helps to significantly increase the sensitivity and specificity of your sequence analysis. Typical applications for the Ion PI™ Hi-Q™ workflow are exome sequencing, gene panel sequencing, and transcriptome analysis. Kit components include sequencing supplies, enzymes, nucleotides, spike-in sequencing controls, and sequencing reagents. The kit contains sufficient reagents and supplies for 4 initializations and 8 sequencing runs on the Ion Proton™ system. (For 8 initializations and 8 runs, see Cat. No. A26772.)
Note: an Ion Proton™ Wash 2 Bottle is required for use of this sequencing kit.
The Ion PI™ Hi-Q™ Sequencing 200 Kit provides:
• The latest advancements in data quality and accuracy using the Ion Proton™ System
• Improved specificity for SNP and insertion/deletion variant calls
• Support for sequencing using the Ion PI™ Chip, enabling up to 80 million reads per run
Rapid and comprehensive genomic profiling
The Ion Proton™ sequencer along with Ion AmpliSeq™ Exome technology and Ion Reporter™ Software enables comprehensive exome sequencing. Delivering up to 80 million reads in a single run, the Ion Proton™ System, used together with the Ion AmpliSeq™ Transcriptome Human Gene Expression Kit, provides fast, cost effective, and easy gene level expression analysis of more than 20,000 RefSeq transcripts. The high quality data produced by the Ion PI™ Hi-Q™ kits paired with the Ion AmpliSeq™ Comprehensive Cancer Panel enables accurate detection of somatic variants in key tumor suppressor and oncogenes in your research.
Single-day sequencing workflow
The fast sequencing and data processing capabilities of the Ion Proton™ System produce sequencing results in as little as a single day. With such rapid turnaround times, the Ion Proton™ System aims to be approximately three times faster than the projected performance of other next-generation sequencing platforms.
Notes
This kit is only compatible with the Ion Proton™ System.
Use this kit with the Ion PI™ Hi-Q™ OT2 200 Kit and Ion PI™ Chip Kit v3.
This kit replaces the Ion PI™ Sequencing 200 Kit v3.
Note: an Ion Proton™ Wash 2 Bottle is required for use of this sequencing kit.
The Ion PI™ Hi-Q™ Sequencing 200 Kit provides:
• The latest advancements in data quality and accuracy using the Ion Proton™ System
• Improved specificity for SNP and insertion/deletion variant calls
• Support for sequencing using the Ion PI™ Chip, enabling up to 80 million reads per run
Rapid and comprehensive genomic profiling
The Ion Proton™ sequencer along with Ion AmpliSeq™ Exome technology and Ion Reporter™ Software enables comprehensive exome sequencing. Delivering up to 80 million reads in a single run, the Ion Proton™ System, used together with the Ion AmpliSeq™ Transcriptome Human Gene Expression Kit, provides fast, cost effective, and easy gene level expression analysis of more than 20,000 RefSeq transcripts. The high quality data produced by the Ion PI™ Hi-Q™ kits paired with the Ion AmpliSeq™ Comprehensive Cancer Panel enables accurate detection of somatic variants in key tumor suppressor and oncogenes in your research.
Single-day sequencing workflow
The fast sequencing and data processing capabilities of the Ion Proton™ System produce sequencing results in as little as a single day. With such rapid turnaround times, the Ion Proton™ System aims to be approximately three times faster than the projected performance of other next-generation sequencing platforms.
Notes
This kit is only compatible with the Ion Proton™ System.
Use this kit with the Ion PI™ Hi-Q™ OT2 200 Kit and Ion PI™ Chip Kit v3.
This kit replaces the Ion PI™ Sequencing 200 Kit v3.
Template Generation System II Kit (Thermo Scientific™)
Thermo Scientific transposon technology is the first technology that allows scientist to insert constructed linear DNA fragment into circular or linear DNA target randomly and very efficiently. The Thermo Scientific Template Generation System II Kit (TGS II Kit) is based on the transposition reaction of the bacteriophage Mu. This system has been simplified to work in vitro, and an artificial Mu transposon, designated as the Entranceposon, has been constructed. The reaction is catalyzed by a single enzyme, MuA Transposase.
TGS II Kit provides the tools for inserting the Entranceposon into foreign DNA at random locations. Insertion of an Entranceposon into unknown target DNA provides primer binding sites for different applications. A simple and fast in vitro transposition reaction takes place in a single reaction tube.
Features:
• Provides templates for DNA sequencing more simply and with less hands-on time than any other method
• Thousands of ready-to-sequence templates from a single transposition reaction: Enough to sequence even large DNA clones (e.g. BAC clones) without fragmentation or subcloning
• Simple mapping of the Entranceposon insertions by colony-PCR or restriction enzyme digestion enables directed sequencing. Fewer sequencing reactions are required to complete a sequence than with the "shotgun" approach
• Universal primers: The sequencing and mapping primers are included in the kit, no need for custom primers.
• Bidirectional sequencing: A single template clone can be used for sequencing the flanking DNA on both sides of the Entranceposon insertion
• Three different antibiotic resistance genes are available in each TGS II Kit
• All components needed as well as detailed instruction manual to perform the reactions are included in the kit. TGS II Kit is an updated version of the TGS I Kit.
Applications
The Template Generation System II Kit is designed for inserting an artificial Mu transposon into unknown target DNA to provide primer binding sites for:
• Rapid sequencing of templates without primer walking or custom primers
• Shotgun sequencing of large DNA clones directly without time-consuming fragmentation and subcloning into a library
• Insertion of PCR priming sites into target DNA with an unknown sequence
• Random insertional mutagenesis of cloned DNA; direct amplification, mapping or sequencing using primers at the insertion site
• Sequencing with transposons vs. traditional sequencing
• Special Applications:
• BAC sequencing
• Transposition reaction into linear target DNA
TGS II Kit provides the tools for inserting the Entranceposon into foreign DNA at random locations. Insertion of an Entranceposon into unknown target DNA provides primer binding sites for different applications. A simple and fast in vitro transposition reaction takes place in a single reaction tube.
Features:
• Provides templates for DNA sequencing more simply and with less hands-on time than any other method
• Thousands of ready-to-sequence templates from a single transposition reaction: Enough to sequence even large DNA clones (e.g. BAC clones) without fragmentation or subcloning
• Simple mapping of the Entranceposon insertions by colony-PCR or restriction enzyme digestion enables directed sequencing. Fewer sequencing reactions are required to complete a sequence than with the "shotgun" approach
• Universal primers: The sequencing and mapping primers are included in the kit, no need for custom primers.
• Bidirectional sequencing: A single template clone can be used for sequencing the flanking DNA on both sides of the Entranceposon insertion
• Three different antibiotic resistance genes are available in each TGS II Kit
• All components needed as well as detailed instruction manual to perform the reactions are included in the kit. TGS II Kit is an updated version of the TGS I Kit.
Applications
The Template Generation System II Kit is designed for inserting an artificial Mu transposon into unknown target DNA to provide primer binding sites for:
• Rapid sequencing of templates without primer walking or custom primers
• Shotgun sequencing of large DNA clones directly without time-consuming fragmentation and subcloning into a library
• Insertion of PCR priming sites into target DNA with an unknown sequence
• Random insertional mutagenesis of cloned DNA; direct amplification, mapping or sequencing using primers at the insertion site
• Sequencing with transposons vs. traditional sequencing
• Special Applications:
• BAC sequencing
• Transposition reaction into linear target DNA
BigDye™ Direct Cycle Sequencing Kit (Applied Biosystems™)
The BigDye® Direct Cycle Sequencing Kit, part of the BigDye® product family, is a complete kit for your PCR and Sequencing needs. This innovative, time-saving kit combines the PCR Purification and Cycle Sequencing Steps, enabling a streamlined protocol and shorter time to results. The kit is available in 24, 100, and 1000-reaction sizes to fit your sequencing throughput needs.
The BigDye® Direct Cycle Sequencing Kit Gives You These Advantages:
• A streamlined, simple procedure
• Performance of all sequencing steps in a single reaction tube
• Fast migration with excellent resolution starting at the 1st base after the gene-specific primer sequence
• Substantial time savings versus previous workflows
Save Time and Get Better Results with a Unique Streamlined Workflow
The BigDye® Direct Cycle Sequencing Kits have been developed to minimize complexity and hands-on time. Compared with previous sequencing procedures, which typically require 5 processing steps and take up to 8 hours, Big Dye® Direct can deliver results in 4 steps taking approximately 5 hours—up to a 40% time savings (Figure 1).
The kit provides reagents for 2 key steps of the sequencing workflow:
• The PCR reaction—using the BigDye® Direct PCR Master Mix and M13 tailed primer (not provided); PCR is performed in approximately 1 hour.
• The unique one-step PCR purification-cycle sequencing reaction—using the BigDye® Direct Sequencing Master Mix and the BigDye® Direct M13 primers provided (M13 tailed primer used for the PCR reaction simplifies the sequencing reaction setup by using 2 sequencing primers independently of the PCR product analyzed).
A One-Tube Protocol Gives You More Confidence
With the BigDye® Direct Cycle Sequencing Kit, all steps from PCR to loading on the capillary can be performed in the same reaction tube without transferring material from one tube to another. By minimizing the number of manual handling steps, the possibility of experimental variability is reduced; therefore, you can have greater confidence in your data.
An Integrated Solution Ideal for Resequencing
The BigDye® Direct Cycle Sequencing Kit is the first product designed to maintain conditions leading to high quality data generation throughout the entire read length with faster run times compared to common sequencing workflows. This is achieved through a unique reagent formulation that allows high-resolution data reads closer to the primer and the use of POP-7™ polymer for faster capillary electrophoresis times (Figure 2). The BigDye® Direct Cycle Sequencing Kit is optimized to work with Applied Biosystems® Genetic Analyzers and the BigDye XTerminator® Purification Kit.
For Research Use Only. Not intended for animal or human therapeutic or diagnostic use
The BigDye® Direct Cycle Sequencing Kit Gives You These Advantages:
• A streamlined, simple procedure
• Performance of all sequencing steps in a single reaction tube
• Fast migration with excellent resolution starting at the 1st base after the gene-specific primer sequence
• Substantial time savings versus previous workflows
Save Time and Get Better Results with a Unique Streamlined Workflow
The BigDye® Direct Cycle Sequencing Kits have been developed to minimize complexity and hands-on time. Compared with previous sequencing procedures, which typically require 5 processing steps and take up to 8 hours, Big Dye® Direct can deliver results in 4 steps taking approximately 5 hours—up to a 40% time savings (Figure 1).
The kit provides reagents for 2 key steps of the sequencing workflow:
• The PCR reaction—using the BigDye® Direct PCR Master Mix and M13 tailed primer (not provided); PCR is performed in approximately 1 hour.
• The unique one-step PCR purification-cycle sequencing reaction—using the BigDye® Direct Sequencing Master Mix and the BigDye® Direct M13 primers provided (M13 tailed primer used for the PCR reaction simplifies the sequencing reaction setup by using 2 sequencing primers independently of the PCR product analyzed).
A One-Tube Protocol Gives You More Confidence
With the BigDye® Direct Cycle Sequencing Kit, all steps from PCR to loading on the capillary can be performed in the same reaction tube without transferring material from one tube to another. By minimizing the number of manual handling steps, the possibility of experimental variability is reduced; therefore, you can have greater confidence in your data.
An Integrated Solution Ideal for Resequencing
The BigDye® Direct Cycle Sequencing Kit is the first product designed to maintain conditions leading to high quality data generation throughout the entire read length with faster run times compared to common sequencing workflows. This is achieved through a unique reagent formulation that allows high-resolution data reads closer to the primer and the use of POP-7™ polymer for faster capillary electrophoresis times (Figure 2). The BigDye® Direct Cycle Sequencing Kit is optimized to work with Applied Biosystems® Genetic Analyzers and the BigDye XTerminator® Purification Kit.
For Research Use Only. Not intended for animal or human therapeutic or diagnostic use
Ion ReproSeq™ PGS Kit with Ion 520™ Chips (24 samples/run) (Ion Torrent™)
The Ion ReproSeq PGS Kit for the Ion S5 System provides all the reagents and materials necessary for a rapid, cost-effective, and comprehensive next-generation sequencing (NGS) workflow for aneuploidy analysis. When used with an Ion S5 sequencing system and Ion Chef System for automated template preparation, the kit enables a simple, end-to-end workflow for the detection of aneuploidies in all 24 chromosomes for research labs interested in reliably assessing pre-implantation embryo biopsy samples.
Using as little as 6 pg of DNA from a single cell, or input from multiple cells, the Ion ReproSeq PGS Kit for the Ion S5 System enables accurate detection of whole chromosome and chromosome arm copy number events (down to 17 Mbp copy number events*) in as little as 10 hours**. Three kit configurations provide comprehensive sample throughput flexibility enabling cost-effective screening of 16, 24, or 96 samples per run. When combined with analysis using Ion Reporter v5.4 or later, data interpretation is enhanced with new functionality such as mosaicism detection, gender masking, and improved data plotting.
Cost-effective NGS workflow with sample throughput flexibility
The Ion ReproSeq PGS Kit for the Ion S5 System delivers scalability and flexibility through three sequencing chips—the Ion 510 Chip, Ion 520 Chip, and Ion 530 Chip—to support various levels of sample throughput. Choose the kit that fits your throughput needs to process 16, 24, and up to 96 samples to achieve throughput flexibility that scales up to improve cost efficiencies and allows your laboratory to respond to sample fluctuation and increases in sample volume.
Rapid turnaround for time-sensitive samples
Leveraging the speed of semiconductor sequencing and automated template preparation by isothermal amplification on the Ion Chef System, the Ion ReproSeq PGS Kit for the Ion S5 System offers a fast benchtop NGS workflow for aneuploidy analysis studies. Go from a single cell or multiple cells isolated from a pre-implantation embryo to analyzed data typically in less than 10 hours when processing up to 16 samples, 10 hours for 24 samples, or 13 hours for up to 96 samples. This rapid turnaround time enables reliable identification of chromosomally normal embryos for time-sensitive genetic research.
The Ion ReproSeq PGS Kit with Ion 520 Chips (24 samples/run) includes all reagents for processing a total of 96 samples through a workflow that includes whole genome amplification, library construction, template preparation and chip loading on the Ion Chef instrument, and sequencing using an Ion S5 System.
The kit contains:
• Ion SingleSeq 96 Kit (Cat. No. A34763)—includes reagents to extract, amplify, and barcode genomic DNA for preparing libraries from pre-implantation embryo biopsy samples, a process that can be completed in less than 4 hours (includes hands-on time)
• Ion S5 ExT Chef Supplies, Ion S5 Chef Solutions, and Ion S5 ExT Chef PGS Reagents—contains pre-packaged single-use cartridges for automated template preparation and chip loading (5 hours, includes hands-on time) whereby library DNA is clonally amplified onto a bead surface through an emulsion-based isothermal amplification reaction
• Ion S5 ExT Sequencing Solutions, Ion S5 ExT Sequencing Reagents—simplifies sequencing with ready-to-use, disposable reagent cartridges for fast and simple instrument set-up, with less than 15 minutes hands-on time, for a sequencing chemistry supporting advanced data quality and accuracy
• Ion 520 Chip Kit (Cat. No. A27761)—contains 4 barcoded chips per kit for sample tracking and sequencing runs using an Ion S5 System
Notes
• This kit is only compatible with the Ion Chef System and Ion S5 systems. The Ion S5 XL System is recommended for rapid turnaround time between sequencing runs. Use of the kits with the Ion S5 System requires significantly more analysis time between runs.
• The Ion ReproSeq PGS Kit is compatible with Torrent Suite Software 5.4 and later. Update your Torrent Server to the latest available version of Torrent Suite Software before using these kits. For data analysis, Ion Reporter Software 5.4 or later is recommended.
*Detecting copy number events <48 Mbp using the Ion ReproSeq™ PGS Kit with Ion 530™ Chips (96 samples/run) requires running ≤24 samples on an Ion 530 Chip
**Sample to analyzed results turnaround time for the Ion ReproSeq PGS Kit with Ion 510 Chips (16 samples/run) is 9.6 hours.
Using as little as 6 pg of DNA from a single cell, or input from multiple cells, the Ion ReproSeq PGS Kit for the Ion S5 System enables accurate detection of whole chromosome and chromosome arm copy number events (down to 17 Mbp copy number events*) in as little as 10 hours**. Three kit configurations provide comprehensive sample throughput flexibility enabling cost-effective screening of 16, 24, or 96 samples per run. When combined with analysis using Ion Reporter v5.4 or later, data interpretation is enhanced with new functionality such as mosaicism detection, gender masking, and improved data plotting.
Cost-effective NGS workflow with sample throughput flexibility
The Ion ReproSeq PGS Kit for the Ion S5 System delivers scalability and flexibility through three sequencing chips—the Ion 510 Chip, Ion 520 Chip, and Ion 530 Chip—to support various levels of sample throughput. Choose the kit that fits your throughput needs to process 16, 24, and up to 96 samples to achieve throughput flexibility that scales up to improve cost efficiencies and allows your laboratory to respond to sample fluctuation and increases in sample volume.
Rapid turnaround for time-sensitive samples
Leveraging the speed of semiconductor sequencing and automated template preparation by isothermal amplification on the Ion Chef System, the Ion ReproSeq PGS Kit for the Ion S5 System offers a fast benchtop NGS workflow for aneuploidy analysis studies. Go from a single cell or multiple cells isolated from a pre-implantation embryo to analyzed data typically in less than 10 hours when processing up to 16 samples, 10 hours for 24 samples, or 13 hours for up to 96 samples. This rapid turnaround time enables reliable identification of chromosomally normal embryos for time-sensitive genetic research.
The Ion ReproSeq PGS Kit with Ion 520 Chips (24 samples/run) includes all reagents for processing a total of 96 samples through a workflow that includes whole genome amplification, library construction, template preparation and chip loading on the Ion Chef instrument, and sequencing using an Ion S5 System.
The kit contains:
• Ion SingleSeq 96 Kit (Cat. No. A34763)—includes reagents to extract, amplify, and barcode genomic DNA for preparing libraries from pre-implantation embryo biopsy samples, a process that can be completed in less than 4 hours (includes hands-on time)
• Ion S5 ExT Chef Supplies, Ion S5 Chef Solutions, and Ion S5 ExT Chef PGS Reagents—contains pre-packaged single-use cartridges for automated template preparation and chip loading (5 hours, includes hands-on time) whereby library DNA is clonally amplified onto a bead surface through an emulsion-based isothermal amplification reaction
• Ion S5 ExT Sequencing Solutions, Ion S5 ExT Sequencing Reagents—simplifies sequencing with ready-to-use, disposable reagent cartridges for fast and simple instrument set-up, with less than 15 minutes hands-on time, for a sequencing chemistry supporting advanced data quality and accuracy
• Ion 520 Chip Kit (Cat. No. A27761)—contains 4 barcoded chips per kit for sample tracking and sequencing runs using an Ion S5 System
Notes
• This kit is only compatible with the Ion Chef System and Ion S5 systems. The Ion S5 XL System is recommended for rapid turnaround time between sequencing runs. Use of the kits with the Ion S5 System requires significantly more analysis time between runs.
• The Ion ReproSeq PGS Kit is compatible with Torrent Suite Software 5.4 and later. Update your Torrent Server to the latest available version of Torrent Suite Software before using these kits. For data analysis, Ion Reporter Software 5.4 or later is recommended.
*Detecting copy number events <48 Mbp using the Ion ReproSeq™ PGS Kit with Ion 530™ Chips (96 samples/run) requires running ≤24 samples on an Ion 530 Chip
**Sample to analyzed results turnaround time for the Ion ReproSeq PGS Kit with Ion 510 Chips (16 samples/run) is 9.6 hours.
5500 W Paired-End Reagents Kit (Applied Biosystems™)
The 5500 W Paired-End Reagents Kit is used for paired-end sequencing runs for ligation sequencing of the F5 Tag. This kit contains sufficient enzyme, dNTPs, and buffers for 6 lanes. In addition to 5500 W Run Cycle Buffers and 5500 W Buffer D (Reset), this kit requires the use of 5500 W Buffer H.
dRhodamine Dye Terminator Ready Reaction Cycle Sequencing Kit (Applied Biosystems™)
The dRhodamine Terminator Cycle Sequencing Kit provides optimized dRhodamine dideoxy terminator chemistries for decreased background noise in a wide array sequencing applications.
• Increase your signal-to-noise ratio.
• Simplify workflows with easy-to-clean-up chemistries.
• Get optimized chemistry for sequencing templates with homo-polymers (AA's, TT's, etc.).
• Obtain clean signal, even peak patterns, and minimal mobility shifts with improved dye terminators.
• Reduce spectrum overlap with narrow dye emission spectra.
Simplify Your Sequencing Workflow
This kit's dRhodamine-dye terminator-based cycle sequencing enables you to sequence DNA rapidly and conveniently. Dye terminators allow you to set up reactions in a single tube, reduce pipetting and liquid handling, and increase sample throughput.
Higher Accuracy, Longer Read Lengths
The dRhodamine Terminator Cycle Sequencing Kit contains the same components as the standard BigDye® Terminator Kit, except dRhodamine (dichlororhodamine) replaces rhodamine terminators, so you get more even peak patterns and less background noise. This alternative chemistry ensures you get higher accuracy, longer read lengths, and more reliable automated sequencing.
Easy to Integrate into Your Workflow
The kit's chemistry requires no new software or instrument recalibration, so you can easily integrate it into your current workflow with minimal changes to your protocols.
For Research Use Only. Not for use in diagnostics procedures.
• Increase your signal-to-noise ratio.
• Simplify workflows with easy-to-clean-up chemistries.
• Get optimized chemistry for sequencing templates with homo-polymers (AA's, TT's, etc.).
• Obtain clean signal, even peak patterns, and minimal mobility shifts with improved dye terminators.
• Reduce spectrum overlap with narrow dye emission spectra.
Simplify Your Sequencing Workflow
This kit's dRhodamine-dye terminator-based cycle sequencing enables you to sequence DNA rapidly and conveniently. Dye terminators allow you to set up reactions in a single tube, reduce pipetting and liquid handling, and increase sample throughput.
Higher Accuracy, Longer Read Lengths
The dRhodamine Terminator Cycle Sequencing Kit contains the same components as the standard BigDye® Terminator Kit, except dRhodamine (dichlororhodamine) replaces rhodamine terminators, so you get more even peak patterns and less background noise. This alternative chemistry ensures you get higher accuracy, longer read lengths, and more reliable automated sequencing.
Easy to Integrate into Your Workflow
The kit's chemistry requires no new software or instrument recalibration, so you can easily integrate it into your current workflow with minimal changes to your protocols.
For Research Use Only. Not for use in diagnostics procedures.
