Shop All Dialysis Membranes, Tubing, Cassettes & Devices
Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 70 mL (Thermo Scientific™)
Thermo Scientific Slide-A-Lyzer G2 Dialysis Cassettes (20K MWCO) provide maximum convenience, flexibility and performance for sample dialysis.
Features of Slide-A-Lyzer G2 Dialysis Cassettes (20K MWCO):
• Easy-loading—pipette-accessible for easy sample loading and retrieval
• Self-floating—integrated air chambers eliminate the need for float buoys
• Sturdy construction—ensures the highest possible sample integrity and protection
• Superior design—thoroughly researched and tested to provide fast and consistent dialysis with maximum sample recovery
• Multiple sizes—five cassette capacities to optimally match 0.25 to 70 mL sample volumes
• Versatile—ideal for removing low molecular-weight contaminants, performing buffer exchange and desalting
The 20K molecular-weight cut off (MWCO) cassettes use a cellulose membrane that is rated to completely retain proteins and other macromolecules that are larger than 20,000 daltons while allowing removal of buffer salts and small contaminants. The cassette membrane is composed of low-binding regenerated cellulose. Membranes and cassettes are manufactured using clean-room conditions to ensure that are contaminant-free.
The Slide-A-Lyzer G2 Dialysis cassettes are available in five precise membrane MWCOs (2K, 3.5K, 7K, 10K and 20K) for dialyzing sample volumes up to 70 mL. The Slide-A-Lyzer G2 Dialysis Cassettes allow samples to be directly injected with a syringe or with a pipette through the unique sample port. The Slide-A-Lyzer Cassette design allows air to be removed from inside the cassette to maximize the surface area-to-volume ratio, providing better dialysis performance than dialysis tubing and tube-based systems. The sample is easily recovered through either the pipette or syringe sample ports.
More Product Data
• Separation characteristics of dialysis membranes
Related Products
Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 3 mL
Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 0.5 mL
Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 15 mL
Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 30 mL
Features of Slide-A-Lyzer G2 Dialysis Cassettes (20K MWCO):
• Easy-loading—pipette-accessible for easy sample loading and retrieval
• Self-floating—integrated air chambers eliminate the need for float buoys
• Sturdy construction—ensures the highest possible sample integrity and protection
• Superior design—thoroughly researched and tested to provide fast and consistent dialysis with maximum sample recovery
• Multiple sizes—five cassette capacities to optimally match 0.25 to 70 mL sample volumes
• Versatile—ideal for removing low molecular-weight contaminants, performing buffer exchange and desalting
The 20K molecular-weight cut off (MWCO) cassettes use a cellulose membrane that is rated to completely retain proteins and other macromolecules that are larger than 20,000 daltons while allowing removal of buffer salts and small contaminants. The cassette membrane is composed of low-binding regenerated cellulose. Membranes and cassettes are manufactured using clean-room conditions to ensure that are contaminant-free.
The Slide-A-Lyzer G2 Dialysis cassettes are available in five precise membrane MWCOs (2K, 3.5K, 7K, 10K and 20K) for dialyzing sample volumes up to 70 mL. The Slide-A-Lyzer G2 Dialysis Cassettes allow samples to be directly injected with a syringe or with a pipette through the unique sample port. The Slide-A-Lyzer Cassette design allows air to be removed from inside the cassette to maximize the surface area-to-volume ratio, providing better dialysis performance than dialysis tubing and tube-based systems. The sample is easily recovered through either the pipette or syringe sample ports.
More Product Data
• Separation characteristics of dialysis membranes
Related Products
Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 3 mL
Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 0.5 mL
Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 15 mL
Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 30 mL
Slide-A-Lyzer™ Dialysis Cassette Kit, 7K MWCO, 12 mL (Thermo Scientific™)
Thermo Scientific Slide-A-Lyzer Dialysis Cassettes (7K MWCO) facilitate simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules that are larger than 7000 daltons.
The 12 mL size is convenient for many preparative applications and the kit format contains syringes, needles, and float buoys for convenience.
Features of Slide-A-Lyzer Dialysis Cassettes:
• Easy to use—no knots or clamps are needed; just inject sample into cassette and begin dialysis
• Fast dialysis —flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
• High recovery—rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
• Three cassette sizes —select the cassette that best suits your sample volume
• Color-coded —green cassette frame specifies cassettes with the 7000 MW pore size membrane
7K Dialysis Membrane Specifications:
• Composition: Regenerated cellulose synthesized by the Viscose method
• Thickness: 0.9 to 1.2mil (22.5 to 30 µM)
• Hydration Required Before Use: 30 seconds for small-volume samples
• Glycerol Content: 13%
• Sulfur Content: 0.1 to 0.15%
• Heavy Metals Content: Trace
Available in three different cassette sizes to accommodate sample volumes between 0.1 and 12 mL, these devices are easier to use than traditional dialysis tubing, providing both faster buffer exchange and more complete sample recovery. Slide-A-Lyzer Cassettes can be used for a wide range of applications including low molecular-weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.
Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.
More Product Data
• Separation characteristics of dialysis membranes
Related Products
Slide-A-Lyzer™ Dialysis Cassettes, 7K MWCO, 3 mL
Slide-A-Lyzer™ Dialysis Cassette Kit, 7K MWCO, 3 mL
Slide-A-Lyzer™ Dialysis Cassettes, 7K MWCO, 0.5 mL
Slide-A-Lyzer™ Dialysis Cassettes, 7K MWCO, 12 mL
The 12 mL size is convenient for many preparative applications and the kit format contains syringes, needles, and float buoys for convenience.
Features of Slide-A-Lyzer Dialysis Cassettes:
• Easy to use—no knots or clamps are needed; just inject sample into cassette and begin dialysis
• Fast dialysis —flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
• High recovery—rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
• Three cassette sizes —select the cassette that best suits your sample volume
• Color-coded —green cassette frame specifies cassettes with the 7000 MW pore size membrane
7K Dialysis Membrane Specifications:
• Composition: Regenerated cellulose synthesized by the Viscose method
• Thickness: 0.9 to 1.2mil (22.5 to 30 µM)
• Hydration Required Before Use: 30 seconds for small-volume samples
• Glycerol Content: 13%
• Sulfur Content: 0.1 to 0.15%
• Heavy Metals Content: Trace
Available in three different cassette sizes to accommodate sample volumes between 0.1 and 12 mL, these devices are easier to use than traditional dialysis tubing, providing both faster buffer exchange and more complete sample recovery. Slide-A-Lyzer Cassettes can be used for a wide range of applications including low molecular-weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.
Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.
More Product Data
• Separation characteristics of dialysis membranes
Related Products
Slide-A-Lyzer™ Dialysis Cassettes, 7K MWCO, 3 mL
Slide-A-Lyzer™ Dialysis Cassette Kit, 7K MWCO, 3 mL
Slide-A-Lyzer™ Dialysis Cassettes, 7K MWCO, 0.5 mL
Slide-A-Lyzer™ Dialysis Cassettes, 7K MWCO, 12 mL
Slide-A-Lyzer™ Dialysis Cassettes, 7K MWCO, 12 mL (Thermo Scientific™)
Thermo Scientific Slide-A-Lyzer Dialysis Cassettes (7K MWCO) facilitate simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules that are larger than 7000 daltons.
The 12 mL size is convenient for many preparative applications
Features of Slide-A-Lyzer Dialysis Cassettes:
• Easy to use—no knots or clamps are needed; just inject sample into cassette and begin dialysis
• Fast dialysis —flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
• High recovery—rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
• Three cassette sizes —select the cassette that best suits your sample volume
• Color-coded —green cassette frame specifies cassettes with the 7000 MW pore size membrane
7K Dialysis Membrane Specifications:
• Composition: Regenerated cellulose synthesized by the Viscose method
• Thickness: 0.9 to 1.2mil (22.5 to 30 µM)
• Hydration Required Before Use: 30 seconds for small-volume samples
• Glycerol Content: 13%
• Sulfur Content: 0.1 to 0.15%
• Heavy Metals Content: Trace
Available in three different cassette sizes to accommodate sample volumes between 0.1 and 12 mL, these devices are easier to use than traditional dialysis tubing, providing both faster buffer exchange and more complete sample recovery. Slide-A-Lyzer Cassettes can be used for a wide range of applications including low molecular-weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.
Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.
More Product Data
• Separation characteristics of dialysis membranes
Related Products
Slide-A-Lyzer™ Dialysis Cassettes, 7K MWCO, 3 mL
Slide-A-Lyzer™ Dialysis Cassette Kit, 7K MWCO, 3 mL
Slide-A-Lyzer™ Dialysis Cassettes, 7K MWCO, 0.5 mL
Slide-A-Lyzer™ Dialysis Cassette Kit, 7K MWCO, 12 mL
The 12 mL size is convenient for many preparative applications
Features of Slide-A-Lyzer Dialysis Cassettes:
• Easy to use—no knots or clamps are needed; just inject sample into cassette and begin dialysis
• Fast dialysis —flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
• High recovery—rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
• Three cassette sizes —select the cassette that best suits your sample volume
• Color-coded —green cassette frame specifies cassettes with the 7000 MW pore size membrane
7K Dialysis Membrane Specifications:
• Composition: Regenerated cellulose synthesized by the Viscose method
• Thickness: 0.9 to 1.2mil (22.5 to 30 µM)
• Hydration Required Before Use: 30 seconds for small-volume samples
• Glycerol Content: 13%
• Sulfur Content: 0.1 to 0.15%
• Heavy Metals Content: Trace
Available in three different cassette sizes to accommodate sample volumes between 0.1 and 12 mL, these devices are easier to use than traditional dialysis tubing, providing both faster buffer exchange and more complete sample recovery. Slide-A-Lyzer Cassettes can be used for a wide range of applications including low molecular-weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.
Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.
More Product Data
• Separation characteristics of dialysis membranes
Related Products
Slide-A-Lyzer™ Dialysis Cassettes, 7K MWCO, 3 mL
Slide-A-Lyzer™ Dialysis Cassette Kit, 7K MWCO, 3 mL
Slide-A-Lyzer™ Dialysis Cassettes, 7K MWCO, 0.5 mL
Slide-A-Lyzer™ Dialysis Cassette Kit, 7K MWCO, 12 mL
Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 0.5 mL (Thermo Scientific™)
Thermo Scientific Slide-A-Lyzer G2 Dialysis Cassettes (20K MWCO) provide maximum convenience, flexibility and performance for sample dialysis.
Features of Slide-A-Lyzer G2 Dialysis Cassettes (20K MWCO):
• Easy-loading—pipette-accessible for easy sample loading and retrieval
• Self-floating—integrated air chambers eliminate the need for float buoys
• Sturdy construction—ensures the highest possible sample integrity and protection
• Superior design—thoroughly researched and tested to provide fast and consistent dialysis with maximum sample recovery
• Multiple sizes—five cassette capacities to optimally match 0.25 to 70 mL sample volumes
• Versatile—ideal for removing low molecular-weight contaminants, performing buffer exchange and desalting
The 20K molecular-weight cut off (MWCO) cassettes use a cellulose membrane that is rated to completely retain proteins and other macromolecules that are larger than 20,000 daltons while allowing removal of buffer salts and small contaminants. The cassette membrane is composed of low-binding regenerated cellulose. Membranes and cassettes are manufactured using clean-room conditions to ensure that are contaminant-free.
The Slide-A-Lyzer G2 Dialysis cassettes are available in five precise membrane MWCOs (2K, 3.5K, 7K, 10K and 20K) for dialyzing sample volumes up to 70 mL. The Slide-A-Lyzer G2 Dialysis Cassettes allow samples to be directly injected with a syringe or with a pipette through the unique sample port. The Slide-A-Lyzer Cassette design allows air to be removed from inside the cassette to maximize the surface area-to-volume ratio, providing better dialysis performance than dialysis tubing and tube-based systems. The sample is easily recovered through either the pipette or syringe sample ports.
More Product Data
• Separation characteristics of dialysis membranes
Related Products
Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 3 mL
Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 15 mL
Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 30 mL
Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 70 mL
Features of Slide-A-Lyzer G2 Dialysis Cassettes (20K MWCO):
• Easy-loading—pipette-accessible for easy sample loading and retrieval
• Self-floating—integrated air chambers eliminate the need for float buoys
• Sturdy construction—ensures the highest possible sample integrity and protection
• Superior design—thoroughly researched and tested to provide fast and consistent dialysis with maximum sample recovery
• Multiple sizes—five cassette capacities to optimally match 0.25 to 70 mL sample volumes
• Versatile—ideal for removing low molecular-weight contaminants, performing buffer exchange and desalting
The 20K molecular-weight cut off (MWCO) cassettes use a cellulose membrane that is rated to completely retain proteins and other macromolecules that are larger than 20,000 daltons while allowing removal of buffer salts and small contaminants. The cassette membrane is composed of low-binding regenerated cellulose. Membranes and cassettes are manufactured using clean-room conditions to ensure that are contaminant-free.
The Slide-A-Lyzer G2 Dialysis cassettes are available in five precise membrane MWCOs (2K, 3.5K, 7K, 10K and 20K) for dialyzing sample volumes up to 70 mL. The Slide-A-Lyzer G2 Dialysis Cassettes allow samples to be directly injected with a syringe or with a pipette through the unique sample port. The Slide-A-Lyzer Cassette design allows air to be removed from inside the cassette to maximize the surface area-to-volume ratio, providing better dialysis performance than dialysis tubing and tube-based systems. The sample is easily recovered through either the pipette or syringe sample ports.
More Product Data
• Separation characteristics of dialysis membranes
Related Products
Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 3 mL
Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 15 mL
Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 30 mL
Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 70 mL
Slide-A-Lyzer™ Dialysis Cassette Kit, 10K MWCO, 12 mL (Thermo Scientific™)
Thermo Scientific Slide-A-Lyzer Dialysis Cassettes (10K MWCO) facilitate simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules that are larger than 10,000 daltons.
The 12 mL size is convenient for preparative applications and the kit format contains syringes, needles, and float buoys for convenience.
Features of Slide-A-Lyzer Dialysis Cassettes:
• Easy to use—no knots or clamps are needed; just inject sample into cassette and begin dialysis
• Fast dialysis—flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
• High recovery—rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
• Four cassette sizes—select the cassette that best suits your sample volume
• Color-coded—orange cassette frame specifies cassettes with the 10,000 MW pore size membrane
• Gamma-irradiated option—gamma-irradiated cassettes are available for applications requiring conditions that minimize the risk of sample contamination
10K Dialysis Membrane Specifications:
• Composition: Regenerated cellulose synthesized by the Viscose method
• Thickness: 0.9 to 1.2mil (22.5 to 30 µM)
• Hydration Required Before Use: 30 seconds for small-volume samples
• Glycerol Content: 21%
• Sulfur Content: 0.05%
• Heavy Metals Content: Trace
Slide-A-Lyzer Dialysis cassettes are available in four different sizes to accommodate sample volumes between 0.1 and 30 mL. These devices are easier to use than traditional dialysis tubing, providing both faster buffer exchange and more complete sample recovery. Slide-A-Lyzer Cassettes can be used for a wide range of applications including low molecular-weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.
Gamma-irradiated Dialysis Cassettes:
Gamma-irradiated Slide-A-Lyzer Dialysis Cassettes are ideal for researchers culturing cells and microorganisms; purifying viruses, DNA and RNA; or performing sample preparation for other applications requiring conditions that minimize the risk of sample contamination. The gamma-irradiated cassettes are treated with 20-36 kGy of gamma radiation, a dosage that has been determined to sterilize the units based on both trypticase soy broth medium for identifying total growth and fluid thioglycollate medium for identifying anaerobic growth. However, a confirmation of sterility is not provided for individual lots, as no further sterility testing is conducted post irradiation.
Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.
More Product Data
• Separation characteristics of dialysis membranes
Related Products
Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 12 mL
Slide-A-Lyzer™ Dialysis Cassettes, gamma-irradiated, 10K MWCO, 12 mL
Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 3 mL
Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 30 mL
The 12 mL size is convenient for preparative applications and the kit format contains syringes, needles, and float buoys for convenience.
Features of Slide-A-Lyzer Dialysis Cassettes:
• Easy to use—no knots or clamps are needed; just inject sample into cassette and begin dialysis
• Fast dialysis—flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
• High recovery—rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
• Four cassette sizes—select the cassette that best suits your sample volume
• Color-coded—orange cassette frame specifies cassettes with the 10,000 MW pore size membrane
• Gamma-irradiated option—gamma-irradiated cassettes are available for applications requiring conditions that minimize the risk of sample contamination
10K Dialysis Membrane Specifications:
• Composition: Regenerated cellulose synthesized by the Viscose method
• Thickness: 0.9 to 1.2mil (22.5 to 30 µM)
• Hydration Required Before Use: 30 seconds for small-volume samples
• Glycerol Content: 21%
• Sulfur Content: 0.05%
• Heavy Metals Content: Trace
Slide-A-Lyzer Dialysis cassettes are available in four different sizes to accommodate sample volumes between 0.1 and 30 mL. These devices are easier to use than traditional dialysis tubing, providing both faster buffer exchange and more complete sample recovery. Slide-A-Lyzer Cassettes can be used for a wide range of applications including low molecular-weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.
Gamma-irradiated Dialysis Cassettes:
Gamma-irradiated Slide-A-Lyzer Dialysis Cassettes are ideal for researchers culturing cells and microorganisms; purifying viruses, DNA and RNA; or performing sample preparation for other applications requiring conditions that minimize the risk of sample contamination. The gamma-irradiated cassettes are treated with 20-36 kGy of gamma radiation, a dosage that has been determined to sterilize the units based on both trypticase soy broth medium for identifying total growth and fluid thioglycollate medium for identifying anaerobic growth. However, a confirmation of sterility is not provided for individual lots, as no further sterility testing is conducted post irradiation.
Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.
More Product Data
• Separation characteristics of dialysis membranes
Related Products
Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 12 mL
Slide-A-Lyzer™ Dialysis Cassettes, gamma-irradiated, 10K MWCO, 12 mL
Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 3 mL
Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 30 mL
Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 3 mL (Thermo Scientific™)
Thermo Scientific Slide-A-Lyzer G2 Dialysis Cassettes (20K MWCO) provide maximum convenience, flexibility and performance for sample dialysis.
Features of Slide-A-Lyzer G2 Dialysis Cassettes (20K MWCO):
• Easy-loading—pipette-accessible for easy sample loading and retrieval
• Self-floating—integrated air chambers eliminate the need for float buoys
• Sturdy construction—ensures the highest possible sample integrity and protection
• Superior design—thoroughly researched and tested to provide fast and consistent dialysis with maximum sample recovery
• Multiple sizes—five cassette capacities to optimally match 0.25 to 70 mL sample volumes
• Versatile—ideal for removing low molecular-weight contaminants, performing buffer exchange and desalting
The 20K molecular-weight cut off (MWCO) cassettes use a cellulose membrane that is rated to completely retain proteins and other macromolecules that are larger than 20,000 daltons while allowing removal of buffer salts and small contaminants. The cassette membrane is composed of low-binding regenerated cellulose. Membranes and cassettes are manufactured using clean-room conditions to ensure that are contaminant-free.
The Slide-A-Lyzer G2 Dialysis cassettes are available in five precise membrane MWCOs (2K, 3.5K, 7K, 10K and 20K) for dialyzing sample volumes up to 70 mL. The Slide-A-Lyzer G2 Dialysis Cassettes allow samples to be directly injected with a syringe or with a pipette through the unique sample port. The Slide-A-Lyzer Cassette design allows air to be removed from inside the cassette to maximize the surface area-to-volume ratio, providing better dialysis performance than dialysis tubing and tube-based systems. The sample is easily recovered through either the pipette or syringe sample ports.
More Product Data
• Separation characteristics of dialysis membranes
Related Products
Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 0.5 mL
Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 15 mL
Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 30 mL
Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 70 mL
Features of Slide-A-Lyzer G2 Dialysis Cassettes (20K MWCO):
• Easy-loading—pipette-accessible for easy sample loading and retrieval
• Self-floating—integrated air chambers eliminate the need for float buoys
• Sturdy construction—ensures the highest possible sample integrity and protection
• Superior design—thoroughly researched and tested to provide fast and consistent dialysis with maximum sample recovery
• Multiple sizes—five cassette capacities to optimally match 0.25 to 70 mL sample volumes
• Versatile—ideal for removing low molecular-weight contaminants, performing buffer exchange and desalting
The 20K molecular-weight cut off (MWCO) cassettes use a cellulose membrane that is rated to completely retain proteins and other macromolecules that are larger than 20,000 daltons while allowing removal of buffer salts and small contaminants. The cassette membrane is composed of low-binding regenerated cellulose. Membranes and cassettes are manufactured using clean-room conditions to ensure that are contaminant-free.
The Slide-A-Lyzer G2 Dialysis cassettes are available in five precise membrane MWCOs (2K, 3.5K, 7K, 10K and 20K) for dialyzing sample volumes up to 70 mL. The Slide-A-Lyzer G2 Dialysis Cassettes allow samples to be directly injected with a syringe or with a pipette through the unique sample port. The Slide-A-Lyzer Cassette design allows air to be removed from inside the cassette to maximize the surface area-to-volume ratio, providing better dialysis performance than dialysis tubing and tube-based systems. The sample is easily recovered through either the pipette or syringe sample ports.
More Product Data
• Separation characteristics of dialysis membranes
Related Products
Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 0.5 mL
Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 15 mL
Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 30 mL
Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 70 mL
Slide-A-Lyzer™ Dialysis Flotation Disks (Thermo Scientific™)
Handy floatation disks are for use with each Thermo Scientific Slide-A-Lyzer Dialysis Flask. When attached to the neck of the flask, the floatation disk allows the flask to float in a vertical position for simple and safe handling.
More Product Data
• Separation characteristics of dialysis membranes
Related Products
Slide-A-Lyzer™ Dialysis Flasks, 10K MWCO, 250 mL
Slide-A-Lyzer™ Dialysis Flasks, 2K MWCO, 250 mL
Slide-A-Lyzer™ Dialysis Flasks, 3.5K MWCO, 230 mL
Slide-A-Lyzer™ Dialysis Flasks, 20K MWCO, 250 mL
More Product Data
• Separation characteristics of dialysis membranes
Related Products
Slide-A-Lyzer™ Dialysis Flasks, 10K MWCO, 250 mL
Slide-A-Lyzer™ Dialysis Flasks, 2K MWCO, 250 mL
Slide-A-Lyzer™ Dialysis Flasks, 3.5K MWCO, 230 mL
Slide-A-Lyzer™ Dialysis Flasks, 20K MWCO, 250 mL
Slide-A-Lyzer™ MINI Dialysis Device, 20K MWCO, 0.1 mL (Thermo Scientific™)
Thermo Scientific Slide-A-Lyzer MINI Dialysis Devices, 20K MWCO are disposable polypropylene cups with integrated, low-binding membranes for dialysis and high recovery of proteins and macromolecules >20kDa in volumes from 10 µL to 2 mL.
Features of Slide-A-Lyzer MINI Dialysis Device, 20K MWCO, 2 mL:
• Three convenient sizes – dialysis devices for 0.1, 0.5 and 2 mL samples fit standard 1.5 mL-micro centrifuge, 15 mL- and 50 mL-conical tubes, respectively
• Excellent sample recoveries – low-binding plastic and small membrane surface area minimize sample loss compared to filtration and resin systems
• One-step protocol – pipette sample into the MINI Device and place in tube containing the dialysis buffer; no laborious assembly, device preparation or expensive equipment are required
• 100% leak-tested – innovative design does not permit "wicking" that can occur in home-made devices
• Time savings – performs desalting of nucleic acids and proteins (>95% recovery) in less than 15 minutes, which improves sample resolution, quantification and conjugation
These 20K MWCO dialysis devices are available in three sizes (0.1, 0.5 and 2 mL capacities), and are designed to allow easy sample addition and removal using a standard laboratory pipette. The MINI Devices are effective and convenient for accomplishing typical sample desalting or buffer exchange in 4 to 8 hours. Unlike other small sample separation devices, there is no equipment to assemble, no need for syringe adapters and no laborious steps necessary to manipulate the small volume sample. The regenerated cellulose membrane is compatible with a number of common chemicals and buffers.
Slide-A-Lyzer MINI Dialysis Devices were developed as a convenient means for dialysing small-volume samples of nucleic acids and proteins without undue sample loss. Low molecular weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, micro conjugation reactions, and concentration can be accomplished with these devices. Slide-A-Lyzer MINI Dialysis Devices are manufactured in a HEPA filter/clean environment and auto-bagged after assembly to ensure that they are free of contaminants and ready to use.
The 0.5 mL and 2 mL MINI Dialysis Devices fit in and are capped by inserting them into the included 15 mL and 50 mL conical tubes, respectively. The tubes can be securely placed in standard laboratory racks or shakers; this feature saves space, minimizes the risk of spills or contamination, and eliminates the need for floats or large beakers of buffer. Using these MINI Dialysis Devices, low molecular weight contaminant removal, buffer exchange and desalting, can be accomplished within 4 to 8 hours with efficiencies, rates, and recoveries very similar to conventional dialysis using a large dialysate volume (view data).
The 0.1 mL MINI Dialysis Devices are designed to be capped (caps included) and inserted into standard 1.5 mL micro centrifuge tubes. Alternatively, as many as 25 MINI Devices can be placed in a Slide-A-Lyzer MINI Dialysis Device Float for dialysis of multiple inside a large beaker. Using the 0.1 mL MINI Device, 100 µL of pH 2.8 glycine buffer can be equilibrated with 1 liter of pH 9.4 bicarbonate buffer in less than 10 minutes.
As with all dialysis applications, the observed rate of dialysis for each sample used with these devices depends on a variety of factors including membrane to sample volume, temperature and agitation rates; as well as the molecular weight, shape, degree of hydration, and charge of the molecules passing through the membranes and the pH, ionic strength, and solvent characteristic of the buffers being used.
More Product Data
• New dimensions for low-volume sample dialysis
• Separation characteristics of dialysis membranes
Related Products
Float Buoys for 0.1 mL Slide-A-Lyzer™ MINI Dialysis Devices
Slide-A-Lyzer™ MINI Dialysis Device, 20K MWCO, 0.5 mL
Slide-A-Lyzer™ MINI Dialysis Device, 20K MWCO, 2 mL
Features of Slide-A-Lyzer MINI Dialysis Device, 20K MWCO, 2 mL:
• Three convenient sizes – dialysis devices for 0.1, 0.5 and 2 mL samples fit standard 1.5 mL-micro centrifuge, 15 mL- and 50 mL-conical tubes, respectively
• Excellent sample recoveries – low-binding plastic and small membrane surface area minimize sample loss compared to filtration and resin systems
• One-step protocol – pipette sample into the MINI Device and place in tube containing the dialysis buffer; no laborious assembly, device preparation or expensive equipment are required
• 100% leak-tested – innovative design does not permit "wicking" that can occur in home-made devices
• Time savings – performs desalting of nucleic acids and proteins (>95% recovery) in less than 15 minutes, which improves sample resolution, quantification and conjugation
These 20K MWCO dialysis devices are available in three sizes (0.1, 0.5 and 2 mL capacities), and are designed to allow easy sample addition and removal using a standard laboratory pipette. The MINI Devices are effective and convenient for accomplishing typical sample desalting or buffer exchange in 4 to 8 hours. Unlike other small sample separation devices, there is no equipment to assemble, no need for syringe adapters and no laborious steps necessary to manipulate the small volume sample. The regenerated cellulose membrane is compatible with a number of common chemicals and buffers.
Slide-A-Lyzer MINI Dialysis Devices were developed as a convenient means for dialysing small-volume samples of nucleic acids and proteins without undue sample loss. Low molecular weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, micro conjugation reactions, and concentration can be accomplished with these devices. Slide-A-Lyzer MINI Dialysis Devices are manufactured in a HEPA filter/clean environment and auto-bagged after assembly to ensure that they are free of contaminants and ready to use.
The 0.5 mL and 2 mL MINI Dialysis Devices fit in and are capped by inserting them into the included 15 mL and 50 mL conical tubes, respectively. The tubes can be securely placed in standard laboratory racks or shakers; this feature saves space, minimizes the risk of spills or contamination, and eliminates the need for floats or large beakers of buffer. Using these MINI Dialysis Devices, low molecular weight contaminant removal, buffer exchange and desalting, can be accomplished within 4 to 8 hours with efficiencies, rates, and recoveries very similar to conventional dialysis using a large dialysate volume (view data).
The 0.1 mL MINI Dialysis Devices are designed to be capped (caps included) and inserted into standard 1.5 mL micro centrifuge tubes. Alternatively, as many as 25 MINI Devices can be placed in a Slide-A-Lyzer MINI Dialysis Device Float for dialysis of multiple inside a large beaker. Using the 0.1 mL MINI Device, 100 µL of pH 2.8 glycine buffer can be equilibrated with 1 liter of pH 9.4 bicarbonate buffer in less than 10 minutes.
As with all dialysis applications, the observed rate of dialysis for each sample used with these devices depends on a variety of factors including membrane to sample volume, temperature and agitation rates; as well as the molecular weight, shape, degree of hydration, and charge of the molecules passing through the membranes and the pH, ionic strength, and solvent characteristic of the buffers being used.
More Product Data
• New dimensions for low-volume sample dialysis
• Separation characteristics of dialysis membranes
Related Products
Float Buoys for 0.1 mL Slide-A-Lyzer™ MINI Dialysis Devices
Slide-A-Lyzer™ MINI Dialysis Device, 20K MWCO, 0.5 mL
Slide-A-Lyzer™ MINI Dialysis Device, 20K MWCO, 2 mL
Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 12 mL (Thermo Scientific™)
Thermo Scientific Slide-A-Lyzer Dialysis Cassettes (10K MWCO) facilitate simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules that are larger than 10,000 daltons.
Features of Slide-A-Lyzer Dialysis Cassettes:
• Easy to use—no knots or clamps are needed; just inject sample into cassette and begin dialysis
• Fast dialysis—flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
• High recovery—rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
• Four cassette sizes—select the cassette that best suits your sample volume
• Color-coded—orange cassette frame specifies cassettes with the 10,000 MW pore size membrane
• Gamma-irradiated option—gamma-irradiated cassettes are available for applications requiring conditions that minimize the risk of sample contamination
10K Dialysis Membrane Specifications:
• Composition: Regenerated cellulose synthesized by the Viscose method
• Thickness: 0.9 to 1.2mil (22.5 to 30 µM)
• Hydration Required Before Use: 30 seconds for small-volume samples
• Glycerol Content: 21%
• Sulfur Content: 0.05%
• Heavy Metals Content: Trace
The 12 mL size is convenient for preparative applications
Slide-A-Lyzer Dialysis cassettes are available in four different sizes to accommodate sample volumes between 0.1 and 30 mL. These devices are easier to use than traditional dialysis tubing, providing both faster buffer exchange and more complete sample recovery. Slide-A-Lyzer Cassettes can be used for a wide range of applications including low molecular-weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.
Gamma-irradiated Dialysis Cassettes:
Gamma-irradiated Slide-A-Lyzer Dialysis Cassettes are ideal for researchers culturing cells and microorganisms; purifying viruses, DNA and RNA; or performing sample preparation for other applications requiring conditions that minimize the risk of sample contamination. The gamma-irradiated cassettes are treated with 20-36 kGy of gamma radiation, a dosage that has been determined to sterilize the units based on both trypticase soy broth medium for identifying total growth and fluid thioglycollate medium for identifying anaerobic growth. However, a confirmation of sterility is not provided for individual lots, as no further sterility testing is conducted post irradiation.
Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.
More Product Data
• Separation characteristics of dialysis membranes
Related Products
Slide-A-Lyzer™ Dialysis Cassette Kit, 10K MWCO, 12 mL
Slide-A-Lyzer™ Dialysis Cassettes, gamma-irradiated, 10K MWCO, 12 mL
Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 3 mL
Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 30 mL
Features of Slide-A-Lyzer Dialysis Cassettes:
• Easy to use—no knots or clamps are needed; just inject sample into cassette and begin dialysis
• Fast dialysis—flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
• High recovery—rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
• Four cassette sizes—select the cassette that best suits your sample volume
• Color-coded—orange cassette frame specifies cassettes with the 10,000 MW pore size membrane
• Gamma-irradiated option—gamma-irradiated cassettes are available for applications requiring conditions that minimize the risk of sample contamination
10K Dialysis Membrane Specifications:
• Composition: Regenerated cellulose synthesized by the Viscose method
• Thickness: 0.9 to 1.2mil (22.5 to 30 µM)
• Hydration Required Before Use: 30 seconds for small-volume samples
• Glycerol Content: 21%
• Sulfur Content: 0.05%
• Heavy Metals Content: Trace
The 12 mL size is convenient for preparative applications
Slide-A-Lyzer Dialysis cassettes are available in four different sizes to accommodate sample volumes between 0.1 and 30 mL. These devices are easier to use than traditional dialysis tubing, providing both faster buffer exchange and more complete sample recovery. Slide-A-Lyzer Cassettes can be used for a wide range of applications including low molecular-weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.
Gamma-irradiated Dialysis Cassettes:
Gamma-irradiated Slide-A-Lyzer Dialysis Cassettes are ideal for researchers culturing cells and microorganisms; purifying viruses, DNA and RNA; or performing sample preparation for other applications requiring conditions that minimize the risk of sample contamination. The gamma-irradiated cassettes are treated with 20-36 kGy of gamma radiation, a dosage that has been determined to sterilize the units based on both trypticase soy broth medium for identifying total growth and fluid thioglycollate medium for identifying anaerobic growth. However, a confirmation of sterility is not provided for individual lots, as no further sterility testing is conducted post irradiation.
Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.
More Product Data
• Separation characteristics of dialysis membranes
Related Products
Slide-A-Lyzer™ Dialysis Cassette Kit, 10K MWCO, 12 mL
Slide-A-Lyzer™ Dialysis Cassettes, gamma-irradiated, 10K MWCO, 12 mL
Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 3 mL
Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 30 mL
Slide-A-Lyzer™ MINI Dialysis Device, 3.5K MWCO, 0.1 mL (Thermo Scientific™)
Thermo Scientific Slide-A-Lyzer MINI Dialysis Devices, 3.5K MWCO are disposable polypropylene cups with integrated, low-binding membranes for dialysis and high recovery of proteins and macromolecules >3.5kDa in volumes from 10 µL to 2 mL.
Features of Slide-A-Lyzer MINI Dialysis Device, 3.5K MWCO, 0.1 mL:
• Three convenient sizes – dialysis devices for 0.1, 0.5 and 2 mL samples fit standard 1.5 mL-micro centrifuge, 15 mL- and 50 mL-conical tubes, respectively
• Excellent sample recoveries – low-binding plastic and small membrane surface area minimize sample loss compared to filtration and resin systems
• One-step protocol – pipette sample into the MINI Device and place in tube containing the dialysis buffer; no laborious assembly, device preparation or expensive equipment are required
• 100% leak-tested – innovative design does not permit "wicking" that can occur in home-made devices
• Time savings – performs desalting of nucleic acids and proteins (>95% recovery) in less than 15 minutes, which improves sample resolution, quantification and conjugation
These 3.5K MWCO dialysis devices are available in three sizes (0.1, 0.5 and 2 mL capacities), and are designed to allow easy sample addition and removal using a standard laboratory pipette. The MINI Devices are effective and convenient for accomplishing typical sample desalting or buffer exchange in 4 to 8 hours. Unlike other small sample separation devices, there is no equipment to assemble, no need for syringe adapters and no laborious steps necessary to manipulate the small volume sample. The regenerated cellulose membrane is compatible with a number of common chemicals and buffers.
Slide-A-Lyzer MINI Dialysis Devices were developed as a convenient means for dialysing small-volume samples of nucleic acids and proteins without undue sample loss. Low molecular weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, micro conjugation reactions, and concentration can be accomplished with these devices. Slide-A-Lyzer MINI Dialysis Devices are manufactured in a HEPA filter/clean environment and auto-bagged after assembly to ensure that they are free of contaminants and ready to use.
The 0.5 mL and 2 mL MINI Dialysis Devices fit in and are capped by inserting them into the included 15 mL and 50 mL conical tubes, respectively. The tubes can be securely placed in standard laboratory racks or shakers; this feature saves space, minimizes the risk of spills or contamination, and eliminates the need for floats or large beakers of buffer. Using these MINI Dialysis Devices, low molecular weight contaminant removal, buffer exchange and desalting, can be accomplished within 4 to 8 hours with efficiencies, rates, and recoveries very similar to conventional dialysis using a large dialysate volume (view data).
The 0.1 mL MINI Dialysis Devices are designed to be capped (caps included) and inserted into standard 1.5 mL micro centrifuge tubes. Alternatively, as many as 25 MINI Devices can be placed in a Slide-A-Lyzer MINI Dialysis Device Float for dialysis of multiple inside a large beaker. Using the 0.1 mL MINI Device, 100 µL of pH 2.8 glycine buffer can be equilibrated to 1 liter of pH 9.4 bicarbonate buffer in less than 10 minutes.
As with all dialysis applications, the observed rate of dialysis for each sample used with these devices depends on a variety of factors including membrane to sample volume, temperature and agitation rates; as well as the molecular weight, shape, degree of hydration, and charge of the molecules passing through the membranes and the pH, ionic strength, and solvent characteristic of the buffers being used.
More Product Data
• New dimensions for low-volume sample dialysis
• Separation characteristics of dialysis membranes
Related Products
Float Buoys for 0.1 mL Slide-A-Lyzer™ MINI Dialysis Devices
Slide-A-Lyzer™ MINI Dialysis Device Kit, 3.5K MWCO, 0.1 mL
Slide-A-Lyzer™ MINI Dialysis Device, 3.5K MWCO, 0.5 mL
Slide-A-Lyzer™ MINI Dialysis Device, 3.5K MWCO, 2 mL
Features of Slide-A-Lyzer MINI Dialysis Device, 3.5K MWCO, 0.1 mL:
• Three convenient sizes – dialysis devices for 0.1, 0.5 and 2 mL samples fit standard 1.5 mL-micro centrifuge, 15 mL- and 50 mL-conical tubes, respectively
• Excellent sample recoveries – low-binding plastic and small membrane surface area minimize sample loss compared to filtration and resin systems
• One-step protocol – pipette sample into the MINI Device and place in tube containing the dialysis buffer; no laborious assembly, device preparation or expensive equipment are required
• 100% leak-tested – innovative design does not permit "wicking" that can occur in home-made devices
• Time savings – performs desalting of nucleic acids and proteins (>95% recovery) in less than 15 minutes, which improves sample resolution, quantification and conjugation
These 3.5K MWCO dialysis devices are available in three sizes (0.1, 0.5 and 2 mL capacities), and are designed to allow easy sample addition and removal using a standard laboratory pipette. The MINI Devices are effective and convenient for accomplishing typical sample desalting or buffer exchange in 4 to 8 hours. Unlike other small sample separation devices, there is no equipment to assemble, no need for syringe adapters and no laborious steps necessary to manipulate the small volume sample. The regenerated cellulose membrane is compatible with a number of common chemicals and buffers.
Slide-A-Lyzer MINI Dialysis Devices were developed as a convenient means for dialysing small-volume samples of nucleic acids and proteins without undue sample loss. Low molecular weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, micro conjugation reactions, and concentration can be accomplished with these devices. Slide-A-Lyzer MINI Dialysis Devices are manufactured in a HEPA filter/clean environment and auto-bagged after assembly to ensure that they are free of contaminants and ready to use.
The 0.5 mL and 2 mL MINI Dialysis Devices fit in and are capped by inserting them into the included 15 mL and 50 mL conical tubes, respectively. The tubes can be securely placed in standard laboratory racks or shakers; this feature saves space, minimizes the risk of spills or contamination, and eliminates the need for floats or large beakers of buffer. Using these MINI Dialysis Devices, low molecular weight contaminant removal, buffer exchange and desalting, can be accomplished within 4 to 8 hours with efficiencies, rates, and recoveries very similar to conventional dialysis using a large dialysate volume (view data).
The 0.1 mL MINI Dialysis Devices are designed to be capped (caps included) and inserted into standard 1.5 mL micro centrifuge tubes. Alternatively, as many as 25 MINI Devices can be placed in a Slide-A-Lyzer MINI Dialysis Device Float for dialysis of multiple inside a large beaker. Using the 0.1 mL MINI Device, 100 µL of pH 2.8 glycine buffer can be equilibrated to 1 liter of pH 9.4 bicarbonate buffer in less than 10 minutes.
As with all dialysis applications, the observed rate of dialysis for each sample used with these devices depends on a variety of factors including membrane to sample volume, temperature and agitation rates; as well as the molecular weight, shape, degree of hydration, and charge of the molecules passing through the membranes and the pH, ionic strength, and solvent characteristic of the buffers being used.
More Product Data
• New dimensions for low-volume sample dialysis
• Separation characteristics of dialysis membranes
Related Products
Float Buoys for 0.1 mL Slide-A-Lyzer™ MINI Dialysis Devices
Slide-A-Lyzer™ MINI Dialysis Device Kit, 3.5K MWCO, 0.1 mL
Slide-A-Lyzer™ MINI Dialysis Device, 3.5K MWCO, 0.5 mL
Slide-A-Lyzer™ MINI Dialysis Device, 3.5K MWCO, 2 mL
Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 0.5 mL (Thermo Scientific™)
Thermo Scientific Slide-A-Lyzer Dialysis Cassettes (10K MWCO) facilitate simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules that are larger than 10,000 daltons.
The 0.5 mL size is convenient for low volumel samples
Features of Slide-A-Lyzer Dialysis Cassettes:
• Easy to use—no knots or clamps are needed; just inject sample into cassette and begin dialysis
• Fast dialysis—flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
• High recovery—rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
• Four cassette sizes—select the cassette that best suits your sample volume
• Color-coded—orange cassette frame specifies cassettes with the 10,000 MW pore size membrane
• Gamma-irradiated option—gamma-irradiated cassettes are available for applications requiring conditions that minimize the risk of sample contamination
10K Dialysis Membrane Specifications:
• Composition: Regenerated cellulose synthesized by the Viscose method
• Thickness: 0.9 to 1.2mil (22.5 to 30 µM)
• Hydration Required Before Use: 30 seconds for small-volume samples
• Glycerol Content: 21%
• Sulfur Content: 0.05%
• Heavy Metals Content: Trace
Slide-A-Lyzer Dialysis cassettes are available in four different sizes to accommodate sample volumes between 0.1 and 30 mL. These devices are easier to use than traditional dialysis tubing, providing both faster buffer exchange and more complete sample recovery. Slide-A-Lyzer Cassettes can be used for a wide range of applications including low molecular-weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.Gamma-irradiated Dialysis Cassettes:
Gamma-irradiated Slide-A-Lyzer Dialysis Cassettes are ideal for researchers culturing cells and microorganisms; purifying viruses, DNA and RNA; or performing sample preparation for other applications requiring conditions that minimize the risk of sample contamination. The gamma-irradiated cassettes are treated with 20-36 kGy of gamma radiation, a dosage that has been determined to sterilize the units based on both trypticase soy broth medium for identifying total growth and fluid thioglycollate medium for identifying anaerobic growth. However, a confirmation of sterility is not provided for individual lots, as no further sterility testing is conducted post irradiation.
Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.
More Product Data
• Separation characteristics of dialysis membranes
Related Products
Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 0.5 mL
Slide-A-Lyzer™ Dialysis Cassettes, gamma-irradiated, 10K MWCO, 0.5 mL
Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 3 mL
Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 12 mL
Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 30 mL
The 0.5 mL size is convenient for low volumel samples
Features of Slide-A-Lyzer Dialysis Cassettes:
• Easy to use—no knots or clamps are needed; just inject sample into cassette and begin dialysis
• Fast dialysis—flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
• High recovery—rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
• Four cassette sizes—select the cassette that best suits your sample volume
• Color-coded—orange cassette frame specifies cassettes with the 10,000 MW pore size membrane
• Gamma-irradiated option—gamma-irradiated cassettes are available for applications requiring conditions that minimize the risk of sample contamination
10K Dialysis Membrane Specifications:
• Composition: Regenerated cellulose synthesized by the Viscose method
• Thickness: 0.9 to 1.2mil (22.5 to 30 µM)
• Hydration Required Before Use: 30 seconds for small-volume samples
• Glycerol Content: 21%
• Sulfur Content: 0.05%
• Heavy Metals Content: Trace
Slide-A-Lyzer Dialysis cassettes are available in four different sizes to accommodate sample volumes between 0.1 and 30 mL. These devices are easier to use than traditional dialysis tubing, providing both faster buffer exchange and more complete sample recovery. Slide-A-Lyzer Cassettes can be used for a wide range of applications including low molecular-weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.Gamma-irradiated Dialysis Cassettes:
Gamma-irradiated Slide-A-Lyzer Dialysis Cassettes are ideal for researchers culturing cells and microorganisms; purifying viruses, DNA and RNA; or performing sample preparation for other applications requiring conditions that minimize the risk of sample contamination. The gamma-irradiated cassettes are treated with 20-36 kGy of gamma radiation, a dosage that has been determined to sterilize the units based on both trypticase soy broth medium for identifying total growth and fluid thioglycollate medium for identifying anaerobic growth. However, a confirmation of sterility is not provided for individual lots, as no further sterility testing is conducted post irradiation.
Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.
More Product Data
• Separation characteristics of dialysis membranes
Related Products
Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 0.5 mL
Slide-A-Lyzer™ Dialysis Cassettes, gamma-irradiated, 10K MWCO, 0.5 mL
Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 3 mL
Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 12 mL
Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 30 mL
Rapid Equilibrium Dialysis (RED) Device Single-Use Plate with Inserts, 8K MWCO (Thermo Scientific™)
The Thermo Scientific Pierce RED Device for Rapid Equilibrium Dialysis provides the easiest, fastest and most reliable system for performing plasma protein-binding assays, a critical step in drug development. This product includes single-use plates with 8K MWCO inserts.
Features of the RED Device for Rapid Equilibrium Dialysis:
• Easy and ready to use—disposable tubes require no presoaking, assembly or specialized equipment
• Designed for speed—the high surface-to-volume ratio of the insert design enables equilibrium to be reached in as few as 100 minutes with vigorous agitation or in three to four hours with 200rpm agitation
• Automation-compatible—designed on a standard 96-well plate template suitable for automated liquid handlers
• Flexible and scalable—perform any number of assays (1 to 48 assays per plate) without wasting the entire plate
• Robust—compartmentalized design eliminates potential for cross talk or leakage
• Reproducible and accurate—validated for plasma binding assays, producing results consistent with those reported in literature
• Quality-tested—each lot of inserts is functionally tested in a protein-binding assay for guaranteed performance
The Pierce Device for rapid equilibrium dialysis (RED) was developed in association with the pharmaceutical industry to provide the easiest, fastest and most reliable system for performing plasma protein-binding assays.
The RED Device is an apparatus for performing equilibrium dialysis experiments in a high throughput, automation-compatible format, and consists of disposable inserts and a base plate formatted to a standard microplate footprint. It has been extensively validated for plasma-binding assays and produces results consistent with those reported in the literature. The RED Device also offers significant improvements in automation, time requirements, versatility and product reliability compared to other commercially available equilibrium dialysis systems.
Features of the RED Device Inserts:
Each single-use, disposable insert is made of two side-by-side chambers separated by a vertical cylinder of dialysis membrane (in 8K or 12K MWCO) validated for minimal nonspecific binding. This format requires no extensive assembly steps or specialized equipment, and each chamber or well is easily accessible from the top of the insert after insertion in the base plate. Additionally, the high surface-to-volume ratio of the membrane compartment allows rapid dialysis, where equilibrium can be reached in 4 hours with high levels of reproducibility and accuracy. In many cases, experiments can be completed in less than 100 minutes.
Insert Features:
• Disposable: require no presoaking, assembly, or specialized equipment
• Short incubation time: large dialysis surface area accelerates equilibrium
• 8K MWCO membrane: ideal molecular-weight cutoff for protein-drug binding studies
• 12K MWCO membrane also available for larger drug molecules
• Membrane composition: regenerated cellulose with low glycerol content as a humectant
Features of the RED Device Base Plates:
RED Device Inserts are designed to be used with either the reusable PTFE or disposable high-density polypropylene base plates. Each RED Device Base Plate holds up to 48 RED Device Inserts and has a standard 96-well plate footprint with 9mm x 9mm well spacing to provide compatibility with automated liquid handling systems. In addition, the disposable RED Device Base Plates are available pre-loaded, providing operation convenience for scientists conducting protein-binding applications. No pre-conditioning of the membrane inserts is needed. When using radioactive materials, this single-use plate is easily disposed of to avoid contamination and cleaning. RED Device Inserts and Base Plates are also available separately.
Base Plate Features:
• Microplate footprint: compatible with automated systems for 96-well plates
• Compartmentalized: eliminates potential for crosstalk or leakage
• PTFE construction: eliminates nonspecific binding and risk of contamination
• Accepts 1 to 48 inserts: run exactly the number of assays needed without waste
Applications:
• Determination of free vs. drug bound to plasma proteins
• Pharmacokinetics studies
• Formulation of drug dosage for in vivo studies
• Drug-drug interaction studies
• Selection criteria during drug lead optimization
• Drug partition between plasma and whole blood
• Solubility study
• Dissociation constant determination (Kd)
• Tissue-binding study using tissue homogenate
Determining the extent to which a molecule binds to plasma proteins is a critical phase of pharmaceutical development because it influences compound dosing, drug efficacy, clearance rate and potential for drug interactions. This determination is enabled by equilibrium dialysis, an accepted standard method for reliable estimation of the nonbound drug fraction in plasma. Although it is the preferred method, equilibrium dialysis is generally labor-intensive, time-consuming, cost-prohibitive and difficult to automate. The RED Device for rapid equilibrium dialysis was developed in close association with the pharmaceutical industry to specifically address these issues, accelerating lead optimization and reducing the attrition rate. In addition to plasma protein binding, the device is used for determining drug partition between red blood cell and plasma, protein binding of liver microsomes to improve the correlation between in vitro and in vivo intrinsic clearance, the competition between tissue protein binding against plasma proteins and dissociation constant determination (Kd).
The RED Device has been extensively validated for plasma-binding assays producing results consistent with those reported in the literature. For example, when used to measure warfarin binding to plasma (human and rat) proteins at two concentrations of 1 µM and 10 µM, the RED Device produced results with minimal intra-experimental variability. The Rapid Equilibrium Dialysis (RED) Device offers significant improvements in automation, time requirements, versatility and product reliability compared to other commercially available systems.
Related Products
Rapid Equilibrium Dialysis (RED) Device Inserts, 8K MWCO
Rapid Equilibrium Dialysis (RED) Device Reusable Base Plate
Rapid Equilibrium Dialysis (RED) Device Single-Use Base Plates
Rapid Equilibrium Dialysis (RED) Device Insert Removal Tool
Rapid Equilibrium Dialysis (RED) Device Single-Use Plate with Inserts, 12K MWCO
Features of the RED Device for Rapid Equilibrium Dialysis:
• Easy and ready to use—disposable tubes require no presoaking, assembly or specialized equipment
• Designed for speed—the high surface-to-volume ratio of the insert design enables equilibrium to be reached in as few as 100 minutes with vigorous agitation or in three to four hours with 200rpm agitation
• Automation-compatible—designed on a standard 96-well plate template suitable for automated liquid handlers
• Flexible and scalable—perform any number of assays (1 to 48 assays per plate) without wasting the entire plate
• Robust—compartmentalized design eliminates potential for cross talk or leakage
• Reproducible and accurate—validated for plasma binding assays, producing results consistent with those reported in literature
• Quality-tested—each lot of inserts is functionally tested in a protein-binding assay for guaranteed performance
The Pierce Device for rapid equilibrium dialysis (RED) was developed in association with the pharmaceutical industry to provide the easiest, fastest and most reliable system for performing plasma protein-binding assays.
The RED Device is an apparatus for performing equilibrium dialysis experiments in a high throughput, automation-compatible format, and consists of disposable inserts and a base plate formatted to a standard microplate footprint. It has been extensively validated for plasma-binding assays and produces results consistent with those reported in the literature. The RED Device also offers significant improvements in automation, time requirements, versatility and product reliability compared to other commercially available equilibrium dialysis systems.
Features of the RED Device Inserts:
Each single-use, disposable insert is made of two side-by-side chambers separated by a vertical cylinder of dialysis membrane (in 8K or 12K MWCO) validated for minimal nonspecific binding. This format requires no extensive assembly steps or specialized equipment, and each chamber or well is easily accessible from the top of the insert after insertion in the base plate. Additionally, the high surface-to-volume ratio of the membrane compartment allows rapid dialysis, where equilibrium can be reached in 4 hours with high levels of reproducibility and accuracy. In many cases, experiments can be completed in less than 100 minutes.
Insert Features:
• Disposable: require no presoaking, assembly, or specialized equipment
• Short incubation time: large dialysis surface area accelerates equilibrium
• 8K MWCO membrane: ideal molecular-weight cutoff for protein-drug binding studies
• 12K MWCO membrane also available for larger drug molecules
• Membrane composition: regenerated cellulose with low glycerol content as a humectant
Features of the RED Device Base Plates:
RED Device Inserts are designed to be used with either the reusable PTFE or disposable high-density polypropylene base plates. Each RED Device Base Plate holds up to 48 RED Device Inserts and has a standard 96-well plate footprint with 9mm x 9mm well spacing to provide compatibility with automated liquid handling systems. In addition, the disposable RED Device Base Plates are available pre-loaded, providing operation convenience for scientists conducting protein-binding applications. No pre-conditioning of the membrane inserts is needed. When using radioactive materials, this single-use plate is easily disposed of to avoid contamination and cleaning. RED Device Inserts and Base Plates are also available separately.
Base Plate Features:
• Microplate footprint: compatible with automated systems for 96-well plates
• Compartmentalized: eliminates potential for crosstalk or leakage
• PTFE construction: eliminates nonspecific binding and risk of contamination
• Accepts 1 to 48 inserts: run exactly the number of assays needed without waste
Applications:
• Determination of free vs. drug bound to plasma proteins
• Pharmacokinetics studies
• Formulation of drug dosage for in vivo studies
• Drug-drug interaction studies
• Selection criteria during drug lead optimization
• Drug partition between plasma and whole blood
• Solubility study
• Dissociation constant determination (Kd)
• Tissue-binding study using tissue homogenate
Determining the extent to which a molecule binds to plasma proteins is a critical phase of pharmaceutical development because it influences compound dosing, drug efficacy, clearance rate and potential for drug interactions. This determination is enabled by equilibrium dialysis, an accepted standard method for reliable estimation of the nonbound drug fraction in plasma. Although it is the preferred method, equilibrium dialysis is generally labor-intensive, time-consuming, cost-prohibitive and difficult to automate. The RED Device for rapid equilibrium dialysis was developed in close association with the pharmaceutical industry to specifically address these issues, accelerating lead optimization and reducing the attrition rate. In addition to plasma protein binding, the device is used for determining drug partition between red blood cell and plasma, protein binding of liver microsomes to improve the correlation between in vitro and in vivo intrinsic clearance, the competition between tissue protein binding against plasma proteins and dissociation constant determination (Kd).
The RED Device has been extensively validated for plasma-binding assays producing results consistent with those reported in the literature. For example, when used to measure warfarin binding to plasma (human and rat) proteins at two concentrations of 1 µM and 10 µM, the RED Device produced results with minimal intra-experimental variability. The Rapid Equilibrium Dialysis (RED) Device offers significant improvements in automation, time requirements, versatility and product reliability compared to other commercially available systems.
Related Products
Rapid Equilibrium Dialysis (RED) Device Inserts, 8K MWCO
Rapid Equilibrium Dialysis (RED) Device Reusable Base Plate
Rapid Equilibrium Dialysis (RED) Device Single-Use Base Plates
Rapid Equilibrium Dialysis (RED) Device Insert Removal Tool
Rapid Equilibrium Dialysis (RED) Device Single-Use Plate with Inserts, 12K MWCO
Slide-A-Lyzer™ Dialysis Flasks, 2K MWCO, 250 mL (Thermo Scientific™)
Thermo Scientific Slide-A-Lyzer Dialysis Flasks, 2K MWCO, 250 mL facilitate simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules in sample volumes up to 250 mL.
Features of Slide-A-Lyzer Dialysis Flasks, 2K MWCO, 250 mL:
• Easy to use – Simply pipette or pour sample into flask and begin dialysis
• Fast dialysis – flat flask chamber with two membranes provides high surface-area to volume ratio, enabling dialysis of a 250 mL sample in 8 hours to overnight
• High recovery – rectangular flask design maximizes recovery of entire sample volume via opening at top of flask
• Multiple molecular-weight cut-offs – select the membrane MWCO that best suits your sample's molecular weight
• Color-coded frames – easily identify membrane pore size (MWCO) based on the frame color
Dialysis Membrane Specifications:
• Composition: Regenerated-cellulose
• Thickness: 0.9 to 1.2mil (22.5 to 30μm); 2K MWCO = 2mil (50μm)
• Hydration Required Before Use: 30 seconds to 2 minutes
• Glycerol Content: Trace (10K MWCO content is 21%)
• Sulfur Content: 0.04 to 0.17%
• Heavy metals Content: Trace
Slide-A-Lyzer Dialysis Flasks are very easy to use. Add and remove sample using a serological pipette or by directly pouring the sample through a wide screw-cap opening at the top of the flask. No knots or clips are needed to seal the units. Slide-A-Lyzer Dialysis Flasks are manufactured using clean room conditions and have low-protein binding regenerated cellulose membranes to ensure maximum sample recovery and sample purity. The dialysis flasks are available in distinct colors, corresponding to the pore size (MWCO) of the dialysis membrane: Purple (20,000 daltons), Orange (10,000 daltons), Pink (3500 daltons), and Blue (2000 daltons).
Slide-A-Lyzer Dialysis Flasks are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of an inert plastic body. The membrane and flask materials are compatible with most laboratory buffers.
With Slide-A-Lyzer Dialysis Flasks, typical low-molecular weight contaminant removal, buffer exchange and desalting can be accomplished in 8 hours to overnight. The rate of dialysis depends on a variety of factors, including sample volume, size and shape of the molecule being dialyzed, agitation and temperature. Minimum dialysis time must be determined empirically for each specific system.
More Product Data
• Separation characteristics of dialysis membranes
Related Products
Slide-A-Lyzer™ Dialysis Flasks, 10K MWCO, 250 mL
Slide-A-Lyzer™ Dialysis Flasks, 3.5K MWCO, 230 mL
Slide-A-Lyzer™ Dialysis Flasks, 20K MWCO, 250 mL
Slide-A-Lyzer™ Dialysis Flotation Disks
Features of Slide-A-Lyzer Dialysis Flasks, 2K MWCO, 250 mL:
• Easy to use – Simply pipette or pour sample into flask and begin dialysis
• Fast dialysis – flat flask chamber with two membranes provides high surface-area to volume ratio, enabling dialysis of a 250 mL sample in 8 hours to overnight
• High recovery – rectangular flask design maximizes recovery of entire sample volume via opening at top of flask
• Multiple molecular-weight cut-offs – select the membrane MWCO that best suits your sample's molecular weight
• Color-coded frames – easily identify membrane pore size (MWCO) based on the frame color
Dialysis Membrane Specifications:
• Composition: Regenerated-cellulose
• Thickness: 0.9 to 1.2mil (22.5 to 30μm); 2K MWCO = 2mil (50μm)
• Hydration Required Before Use: 30 seconds to 2 minutes
• Glycerol Content: Trace (10K MWCO content is 21%)
• Sulfur Content: 0.04 to 0.17%
• Heavy metals Content: Trace
Slide-A-Lyzer Dialysis Flasks are very easy to use. Add and remove sample using a serological pipette or by directly pouring the sample through a wide screw-cap opening at the top of the flask. No knots or clips are needed to seal the units. Slide-A-Lyzer Dialysis Flasks are manufactured using clean room conditions and have low-protein binding regenerated cellulose membranes to ensure maximum sample recovery and sample purity. The dialysis flasks are available in distinct colors, corresponding to the pore size (MWCO) of the dialysis membrane: Purple (20,000 daltons), Orange (10,000 daltons), Pink (3500 daltons), and Blue (2000 daltons).
Slide-A-Lyzer Dialysis Flasks are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of an inert plastic body. The membrane and flask materials are compatible with most laboratory buffers.
With Slide-A-Lyzer Dialysis Flasks, typical low-molecular weight contaminant removal, buffer exchange and desalting can be accomplished in 8 hours to overnight. The rate of dialysis depends on a variety of factors, including sample volume, size and shape of the molecule being dialyzed, agitation and temperature. Minimum dialysis time must be determined empirically for each specific system.
More Product Data
• Separation characteristics of dialysis membranes
Related Products
Slide-A-Lyzer™ Dialysis Flasks, 10K MWCO, 250 mL
Slide-A-Lyzer™ Dialysis Flasks, 3.5K MWCO, 230 mL
Slide-A-Lyzer™ Dialysis Flasks, 20K MWCO, 250 mL
Slide-A-Lyzer™ Dialysis Flotation Disks
Slide-A-Lyzer™ Cassette Carousel Float Buoy (for 0.5 mL to 3 mL cassettes) (Thermo Scientific™)
Thermo Scientific Slide-A-Lyzer Cassette Float Buoys are designed for floating 0.5 mL to 3 mL cassettes in dialysate.
These accessories are for use with Slide-A-Lyzer Dialysis Cassettes to remove low molecular weight contaminants and perform buffer exchange, desalting and concentration.
Features of Slide-A-Lyzer Cassette Float Buoys:
• Keeps stir bar from tearing dialysis membrane
• Matched to Slide-A-Lyzer Cassette for a secure fit
• Chemically inert
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Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 0.5 mL
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These accessories are for use with Slide-A-Lyzer Dialysis Cassettes to remove low molecular weight contaminants and perform buffer exchange, desalting and concentration.
Features of Slide-A-Lyzer Cassette Float Buoys:
• Keeps stir bar from tearing dialysis membrane
• Matched to Slide-A-Lyzer Cassette for a secure fit
• Chemically inert
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Slide-A-Lyzer™ MINI Dialysis Device, 10K MWCO, 0.5 mL (Thermo Scientific™)
Thermo Scientific Slide-A-Lyzer MINI Dialysis Devices, 10K MWCO are disposable polypropylene cups with integrated, low-binding membranes for dialysis and high recovery of proteins and macromolecules >10kDa in volumes from 10 µL to 2 mL.
Features of Slide-A-Lyzer MINI Dialysis Device, 10K MWCO, 0.5 mL:
• Three convenient sizes – dialysis devices for 0.1, 0.5 and 2 mL samples fit standard 1.5 mL-micro centrifuge, 15 mL- and 50 mL-conical tubes, respectively
• Excellent sample recoveries – low-binding plastic and small membrane surface area minimize sample loss compared to filtration and resin systems
• One-step protocol – pipette sample into the MINI Device and place in tube containing the dialysis buffer; no laborious assembly, device preparation or expensive equipment are required
• 100% leak-tested – innovative design does not permit "wicking" that can occur in home-made devices
• Time savings – performs desalting of nucleic acids and proteins (>95% recovery) in less than 15 minutes, which improves sample resolution, quantification and conjugation
These 10K MWCO dialysis devices are available in three sizes (0.1, 0.5 and 2 mL capacities), and are designed to allow easy sample addition and removal using a standard laboratory pipette. The MINI Devices are effective and convenient for accomplishing typical sample desalting or buffer exchange in 4 to 8 hours. Unlike other small sample separation devices, there is no equipment to assemble, no need for syringe adapters and no laborious steps necessary to manipulate the small volume sample. The regenerated cellulose membrane is compatible with a number of common chemicals and buffers.
Slide-A-Lyzer MINI Dialysis Devices were developed as a convenient means for dialysing small-volume samples of nucleic acids and proteins without undue sample loss. Low molecular weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, micro conjugation reactions, and concentration can be accomplished with these devices. Slide-A-Lyzer MINI Dialysis Devices are manufactured in a HEPA filter/clean environment and auto-bagged after assembly to ensure that they are free of contaminants and ready to use.
The 0.5 mL and 2 mL MINI Dialysis Devices fit in and are capped by inserting them into the included 15 mL and 50 mL conical tubes, respectively. The tubes can be securely placed in standard laboratory racks or shakers; this feature saves space, minimizes the risk of spills or contamination, and eliminates the need for floats or large beakers of buffer. Using these MINI Dialysis Devices, low molecular weight contaminant removal, buffer exchange and desalting, can be accomplished within 4 to 8 hours with efficiencies, rates, and recoveries very similar to conventional dialysis using a large dialysate volume (view data).
The 0.1 mL MINI Dialysis Devices are designed to be capped (caps included) and inserted into standard 1.5 mL micro centrifuge tubes. Alternatively, as many as 25 MINI Devices can be placed in a Slide-A-Lyzer MINI Dialysis Device Float for dialysis of multiple inside a large beaker. Using the 0.1 mL MINI Device, 100 µL of pH 2.8 glycine buffer can be equilibrated with 1 liter of pH 9.4 bicarbonate buffer in less than 10 minutes.
As with all dialysis applications, the observed rate of dialysis for each sample used with these devices depends on a variety of factors including membrane to sample volume, temperature and agitation rates; as well as the molecular weight, shape, degree of hydration, and charge of the molecules passing through the membranes and the pH, ionic strength, and solvent characteristic of the buffers being used.
More Product Data
• New dimensions for low-volume sample dialysis
• Separation characteristics of dialysis membranes
Related Products
Slide-A-Lyzer™ MINI Dialysis Device, 10K MWCO, 0.1 mL
Slide-A-Lyzer™ MINI Dialysis Device Kit, 10K MWCO, 0.1 mL
Slide-A-Lyzer™ MINI Dialysis Device Kit, 10K MWCO, 0.1 mL
Slide-A-Lyzer™ MINI Dialysis Device, 10K MWCO, 2 mL
Features of Slide-A-Lyzer MINI Dialysis Device, 10K MWCO, 0.5 mL:
• Three convenient sizes – dialysis devices for 0.1, 0.5 and 2 mL samples fit standard 1.5 mL-micro centrifuge, 15 mL- and 50 mL-conical tubes, respectively
• Excellent sample recoveries – low-binding plastic and small membrane surface area minimize sample loss compared to filtration and resin systems
• One-step protocol – pipette sample into the MINI Device and place in tube containing the dialysis buffer; no laborious assembly, device preparation or expensive equipment are required
• 100% leak-tested – innovative design does not permit "wicking" that can occur in home-made devices
• Time savings – performs desalting of nucleic acids and proteins (>95% recovery) in less than 15 minutes, which improves sample resolution, quantification and conjugation
These 10K MWCO dialysis devices are available in three sizes (0.1, 0.5 and 2 mL capacities), and are designed to allow easy sample addition and removal using a standard laboratory pipette. The MINI Devices are effective and convenient for accomplishing typical sample desalting or buffer exchange in 4 to 8 hours. Unlike other small sample separation devices, there is no equipment to assemble, no need for syringe adapters and no laborious steps necessary to manipulate the small volume sample. The regenerated cellulose membrane is compatible with a number of common chemicals and buffers.
Slide-A-Lyzer MINI Dialysis Devices were developed as a convenient means for dialysing small-volume samples of nucleic acids and proteins without undue sample loss. Low molecular weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, micro conjugation reactions, and concentration can be accomplished with these devices. Slide-A-Lyzer MINI Dialysis Devices are manufactured in a HEPA filter/clean environment and auto-bagged after assembly to ensure that they are free of contaminants and ready to use.
The 0.5 mL and 2 mL MINI Dialysis Devices fit in and are capped by inserting them into the included 15 mL and 50 mL conical tubes, respectively. The tubes can be securely placed in standard laboratory racks or shakers; this feature saves space, minimizes the risk of spills or contamination, and eliminates the need for floats or large beakers of buffer. Using these MINI Dialysis Devices, low molecular weight contaminant removal, buffer exchange and desalting, can be accomplished within 4 to 8 hours with efficiencies, rates, and recoveries very similar to conventional dialysis using a large dialysate volume (view data).
The 0.1 mL MINI Dialysis Devices are designed to be capped (caps included) and inserted into standard 1.5 mL micro centrifuge tubes. Alternatively, as many as 25 MINI Devices can be placed in a Slide-A-Lyzer MINI Dialysis Device Float for dialysis of multiple inside a large beaker. Using the 0.1 mL MINI Device, 100 µL of pH 2.8 glycine buffer can be equilibrated with 1 liter of pH 9.4 bicarbonate buffer in less than 10 minutes.
As with all dialysis applications, the observed rate of dialysis for each sample used with these devices depends on a variety of factors including membrane to sample volume, temperature and agitation rates; as well as the molecular weight, shape, degree of hydration, and charge of the molecules passing through the membranes and the pH, ionic strength, and solvent characteristic of the buffers being used.
More Product Data
• New dimensions for low-volume sample dialysis
• Separation characteristics of dialysis membranes
Related Products
Slide-A-Lyzer™ MINI Dialysis Device, 10K MWCO, 0.1 mL
Slide-A-Lyzer™ MINI Dialysis Device Kit, 10K MWCO, 0.1 mL
Slide-A-Lyzer™ MINI Dialysis Device Kit, 10K MWCO, 0.1 mL
Slide-A-Lyzer™ MINI Dialysis Device, 10K MWCO, 2 mL
