Shop All Real-Time PCR Instruments & Accessories
Pharmaceutical Analytics QuantStudio™ 5 Real-Time PCR System, 96-well, 0.2 mL, desktop (Applied Biosystems™)
The Applied Biosystems™ QuantStudio™ 5 Real-Time PCR System for Pharmaceutical Analytics is designed for users who need superior performance, maximum dye versatility, and security options in a real-time PCR system that is affordable and easy to use. The QuantStudio 5 pharmaceutical analytics system is used together with specialized sample preparation and quantitation kits for host cell protein, host cell DNA, and Protein A impurity testing of biological products produced in cell culture.The optimized Design and Analysis software is ideal for both first-time and experienced users. When connected to the Thermo Fisher Cloud, the QuantStudio 5 system provides access to your data wherever and whenever you want. Using proven OptiFlex® technology (featuring 6 decoupled channels and white LED) and featuring six independent Veriflex® temperature zones, the QuantStudio 5 96-well system enables improved data accuracy and sensitivity for a broad range of genomic applications. The QuantStudio 5 system also offers the built-in software features of electronic record security and prevention of unauthorized instrument access to assist with 21 CFR Part 11 compliance.
Features of the QuantStudio 5 system with 96-well block include:
• Proven OptiFlex technology (6 decoupled channels/21 filter combinations with white LED) for trusted results
• User account management, locked workflow, and Pause features for greater control of experiment data
• Built-in software features to support compliance with 21 CFR Part 11 guidelines
• Secure cloud-based software provides increased accessibility and storage options
• Simplified instrument software with interactive touchscreen for greater ease of use
• 96-well 0.2mL format or 96-well 0.1mL format
Thermo Fisher Cloud
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Upload data from the instrument or upload your own files from anywhere you have web access. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use.
Downloads
>> QuantStudio 3/5 Design & Analysis Software | Release Notes | Example Data & Templates
>> QuantStudio 3/5 Firmware | Release Notes
Easy to use
The QuantStudio 5 Real-Time PCR System is compact in form, factory calibrated and is delivered in user-friendly packaging to enable fast and simple installation for immediate use. The interactive touchscreen provides a modern usability experience and the simplified instrument software/pre-optimized protocol templates allow users of any experience level to quickly set up and start a run.
Design and analysis software options:
Option 1: QuantStudio Design and Analysis cloud software
• Supports web browser-based system configuration with PC or MAC computer
• Streamlined software for improved usability and response time
• Enables secure access of your data when and where you want through the cloud
• No software to install, no licenses and no versions to update
Option 2: QuantStudio Design and Analysis desktop software
• Supports traditional co-located computer system configuration on Windows® 7 OS
• Streamlined software for improved usability and response time
• No licenses to purchase
Total control
With built-in software features to support compliance with 21 CFR Part 11 guidelines, user account management, locked workflow and Pause features, the QuantStudio 5 system offers users greater control of experiment data
Multiplex performance results that you can trust
Improved well-to-well and instrument-to-instrument data accuracy is achieved with OptiFlex technology. In the 96-well system this technology features 6 decoupled excitation and emission filter channels, for a total of 21 filter combinations, with white LED.
Sensitive data analysis
Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.
High application versatility
Simple and optimized protocols, reliable assays and reagents, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, gene regulation, and more.
Features of the QuantStudio 5 system with 96-well block include:
• Proven OptiFlex technology (6 decoupled channels/21 filter combinations with white LED) for trusted results
• User account management, locked workflow, and Pause features for greater control of experiment data
• Built-in software features to support compliance with 21 CFR Part 11 guidelines
• Secure cloud-based software provides increased accessibility and storage options
• Simplified instrument software with interactive touchscreen for greater ease of use
• 96-well 0.2mL format or 96-well 0.1mL format
Thermo Fisher Cloud
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Upload data from the instrument or upload your own files from anywhere you have web access. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use.
Downloads
>> QuantStudio 3/5 Design & Analysis Software | Release Notes | Example Data & Templates
>> QuantStudio 3/5 Firmware | Release Notes
Easy to use
The QuantStudio 5 Real-Time PCR System is compact in form, factory calibrated and is delivered in user-friendly packaging to enable fast and simple installation for immediate use. The interactive touchscreen provides a modern usability experience and the simplified instrument software/pre-optimized protocol templates allow users of any experience level to quickly set up and start a run.
Design and analysis software options:
Option 1: QuantStudio Design and Analysis cloud software
• Supports web browser-based system configuration with PC or MAC computer
• Streamlined software for improved usability and response time
• Enables secure access of your data when and where you want through the cloud
• No software to install, no licenses and no versions to update
Option 2: QuantStudio Design and Analysis desktop software
• Supports traditional co-located computer system configuration on Windows® 7 OS
• Streamlined software for improved usability and response time
• No licenses to purchase
Total control
With built-in software features to support compliance with 21 CFR Part 11 guidelines, user account management, locked workflow and Pause features, the QuantStudio 5 system offers users greater control of experiment data
Multiplex performance results that you can trust
Improved well-to-well and instrument-to-instrument data accuracy is achieved with OptiFlex technology. In the 96-well system this technology features 6 decoupled excitation and emission filter channels, for a total of 21 filter combinations, with white LED.
Sensitive data analysis
Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.
High application versatility
Simple and optimized protocols, reliable assays and reagents, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, gene regulation, and more.
ViiA™ 7 Dx Instrument, 96 well, laptop (Applied Biosystems™)
The ViiA™ 7 Dx Instrument puts high-productivity qPCR at your fingertips. With the new OptiFlex™ optical system and 4 interchangeable block formats from 96-well to 384-well to Micro Fludic Array Card, the ViiA™ 7 Dx Instrument is available for in-vitro diagnostics use.
Key product features:
• Proven Applied Biosystems reliability and accuracy
• Using with Micro Fluidic Array Cards provides an integrated workflow and faster results.
• Easy to use – intuitive software, responsive touch-screen, effortless block exchange without the need for any tools
Productivity
Ideal for performing medium- to high-throughput real-time PCR, the ViiA™ 7 Dx Instrument enhances your lab’s productivity:
• Quick block changes: Front access makes it easy to change thermal cycling block formats without having to move attached peripherals such as robots or computers
• Easy touch-screen interface: Instrument touch screen provides one-touch protocols for fast and easy assay setup for a broad range of applications
• Peace of mind: Data recovery capability for 100+ runs
Performance
The ViiA™ 7 Dx Instrument features the new OptiFlex™ System, which provides enhanced fluorescence detection:
• Maximum multiplexing: Six decoupled excitation and emission filter channels for the greatest number of dye combinations and maximum multiplexing capabilities
• Flexible data collection: Multiple ramp method detection formats provide more flexibility for collecting data during a ramp stage
• Precise quantification: Detect as small as 1.5–fold changes in target quantities in singleplex reactions
ViiA™ 7 Dx Software drives value in a number of ways, offering:
• Intuitive interface and innovative design
• Convenient walk-away operation
• Improved data analysis
• Fully compatible with high-throughput environments
Note: The ViiA™ 7 Dx Instrument and consumables are for distribution and use in specific European countries only. Not for use in the USA.
Key product features:
• Proven Applied Biosystems reliability and accuracy
• Using with Micro Fluidic Array Cards provides an integrated workflow and faster results.
• Easy to use – intuitive software, responsive touch-screen, effortless block exchange without the need for any tools
Productivity
Ideal for performing medium- to high-throughput real-time PCR, the ViiA™ 7 Dx Instrument enhances your lab’s productivity:
• Quick block changes: Front access makes it easy to change thermal cycling block formats without having to move attached peripherals such as robots or computers
• Easy touch-screen interface: Instrument touch screen provides one-touch protocols for fast and easy assay setup for a broad range of applications
• Peace of mind: Data recovery capability for 100+ runs
Performance
The ViiA™ 7 Dx Instrument features the new OptiFlex™ System, which provides enhanced fluorescence detection:
• Maximum multiplexing: Six decoupled excitation and emission filter channels for the greatest number of dye combinations and maximum multiplexing capabilities
• Flexible data collection: Multiple ramp method detection formats provide more flexibility for collecting data during a ramp stage
• Precise quantification: Detect as small as 1.5–fold changes in target quantities in singleplex reactions
ViiA™ 7 Dx Software drives value in a number of ways, offering:
• Intuitive interface and innovative design
• Convenient walk-away operation
• Improved data analysis
• Fully compatible with high-throughput environments
Note: The ViiA™ 7 Dx Instrument and consumables are for distribution and use in specific European countries only. Not for use in the USA.
Precision Plate Holder for 7500 Real-Time PCR Systems (Applied Biosystems™)
This precision plate holder is required for use of MicroAmp® EnduraPlate™ optical reaction plates on Applied Biosystems® 7500 real-time PCR systems. It also acommodates MicroAmp® optical reaction plates.
QuantStudio™ Dx Real-Time PCR Instrument with 384-well block (Applied Biosystems™)
The QuantStudio™ Dx Real-Time PCR Instrument with 384-Well Block is a real-time PCR platform that delivers the proven performance, flexibility, expandability, and support needed to meet the evolving needs of the clinical laboratory today and tomorrow. The QuantStudio™ Dx Real-Time PCR Instrument with 384-Well Block offers easy-to-change blocks that can accommodate array cards, as well as 96-well fast and 96-well standard plates, allowing you to select the format that is right for your throughput needs.
Built on a legacy of innovation and excellence in real-time PCR, the QuantStudio™ Dx instrument leverages the best of our portfolio of Applied Biosystems® real-time PCR systems, and refines a platform to specifically meet the needs of the diagnostic laboratory.
Results You Can Trust
The QuantStudio™ Dx Real-Time PCR Instrument’s proven performance, security, and reliability—combined with the outstanding sensitivity and specificity of the Quidel® Molecular Assays—help ensure the right answer is delivered the first time, every time.
Flexibility You Want, Security You Need
The QuantStudio™ Dx Real-Time PCR Instrument with 384-Well Block is designed with easily interchangeable thermal cycling blocks to accommodate your laboratory’s varying needs for throughput, format type, and assay type. In addition to the 384-well format, the instrument also accepts 96-well fast, 96-well standard, and array card formats that can be used for laboratory-developed tests (LDTs) and future IVD assays. Additionally, the instrument can be operated in Test Development Mode, which enables clinical laboratories to develop their own assays or perform clinical research.
Efficient Workflows and Assays
A simplified instrument and assay workflow, as well as intuitive software, make the entire test easy and efficient, from processing to reporting. Plus, it frees up highly trained operators to perform more complex tasks.
QuantStudio™ Dx Software
• Track reagent and patient information for quality control purposes
• Enter reagent information by barcode and upload patient information from Excel files to minimize data entry errors
• Print calibration and maintenance reports for your instrument records
• Included SAE module assists with Security, Auditing, and Electronic signature of records for full data traceability as part of 21 CRF Part 11 compliance
For In Vitro Diagnostic Use. The QuantStudio™ Dx Real-Time PCR Instrument meets the requirements of the in vitro Diagnostic Medical Devices Directive (98/79/EC). The CE IVD-registered QuantStudio™ Dx Real-Time PCR Instrument is for distribution and use in specific countries only. Not available for sale in the United States.
Built on a legacy of innovation and excellence in real-time PCR, the QuantStudio™ Dx instrument leverages the best of our portfolio of Applied Biosystems® real-time PCR systems, and refines a platform to specifically meet the needs of the diagnostic laboratory.
Results You Can Trust
The QuantStudio™ Dx Real-Time PCR Instrument’s proven performance, security, and reliability—combined with the outstanding sensitivity and specificity of the Quidel® Molecular Assays—help ensure the right answer is delivered the first time, every time.
Flexibility You Want, Security You Need
The QuantStudio™ Dx Real-Time PCR Instrument with 384-Well Block is designed with easily interchangeable thermal cycling blocks to accommodate your laboratory’s varying needs for throughput, format type, and assay type. In addition to the 384-well format, the instrument also accepts 96-well fast, 96-well standard, and array card formats that can be used for laboratory-developed tests (LDTs) and future IVD assays. Additionally, the instrument can be operated in Test Development Mode, which enables clinical laboratories to develop their own assays or perform clinical research.
Efficient Workflows and Assays
A simplified instrument and assay workflow, as well as intuitive software, make the entire test easy and efficient, from processing to reporting. Plus, it frees up highly trained operators to perform more complex tasks.
QuantStudio™ Dx Software
• Track reagent and patient information for quality control purposes
• Enter reagent information by barcode and upload patient information from Excel files to minimize data entry errors
• Print calibration and maintenance reports for your instrument records
• Included SAE module assists with Security, Auditing, and Electronic signature of records for full data traceability as part of 21 CRF Part 11 compliance
For In Vitro Diagnostic Use. The QuantStudio™ Dx Real-Time PCR Instrument meets the requirements of the in vitro Diagnostic Medical Devices Directive (98/79/EC). The CE IVD-registered QuantStudio™ Dx Real-Time PCR Instrument is for distribution and use in specific countries only. Not available for sale in the United States.
Pharmaceutical Analytics QuantStudio™ 5 Real-Time PCR System, 384-well (Applied Biosystems™)
The Applied Biosystems™ QuantStudio™ 5 Real-Time PCR System for Pharmaceutical Analytics is designed for users who need superior performance, maximum dye versatility, and security options in a real-time PCR system that is affordable and easy to use. The QuantStudio 5 pharmaceutical analytics system is used together with specialized sample preparation and quantitation kits for host cell protein, host cell DNA, and Protein A impurity testing of biological products produced in cell culture.The optimized Design and Analysis software is ideal for both first-time and experienced users. When connected to the Thermo Fisher Cloud, the QuantStudio 5 system provides access to your data wherever and whenever you want. Using proven OptiFlex® technology (featuring 6 decoupled channels and white LED) and featuring six independent Veriflex® temperature zones, the QuantStudio 5 96-well system enables improved data accuracy and sensitivity for a broad range of genomic applications. The QuantStudio 5 system also offers the built-in software features of electronic record security and prevention of unauthorized instrument access to assist with 21 CFR Part 11 compliance.
Features of the QuantStudio 5 system with 96-well block include:
• Proven OptiFlex technology (6 decoupled channels/21 filter combinations with white LED) for trusted results
• User account management, locked workflow, and Pause features for greater control of experiment data
• Built-in software features to support compliance with 21 CFR Part 11 guidelines
• Secure cloud-based software provides increased accessibility and storage options
• Simplified instrument software with interactive touchscreen for greater ease of use
• 96-well 0.2mL format or 96-well 0.1mL format
Thermo Fisher Cloud
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Upload data from the instrument or upload your own files from anywhere you have web access. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use.
Downloads
>> QuantStudio 3/5 Design & Analysis Software | Release Notes | Example Data & Templates
>> QuantStudio 3/5 Firmware | Release Notes
Easy to use
The QuantStudio 5 Real-Time PCR System is compact in form, factory calibrated and is delivered in user-friendly packaging to enable fast and simple installation for immediate use. The interactive touchscreen provides a modern usability experience and the simplified instrument software/pre-optimized protocol templates allow users of any experience level to quickly set up and start a run.
Design and analysis software options:
Option 1: QuantStudio Design and Analysis cloud software
• Supports web browser-based system configuration with PC or MAC computer
• Streamlined software for improved usability and response time
• Enables secure access of your data when and where you want through the cloud
• No software to install, no licenses and no versions to update
Option 2: QuantStudio Design and Analysis desktop software
• Supports traditional co-located computer system configuration on Windows® 7 OS
• Streamlined software for improved usability and response time
• No licenses to purchase
Total control
With built-in software features to support compliance with 21 CFR Part 11 guidelines, user account management, locked workflow and Pause features, the QuantStudio 5 system offers users greater control of experiment data
Multiplex performance results that you can trust
Improved well-to-well and instrument-to-instrument data accuracy is achieved with OptiFlex technology. In the 96-well system this technology features 6 decoupled excitation and emission filter channels, for a total of 21 filter combinations, with white LED.
Sensitive data analysis
Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.
High application versatility
Simple and optimized protocols, reliable assays and reagents, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, gene regulation, and more.
Features of the QuantStudio 5 system with 96-well block include:
• Proven OptiFlex technology (6 decoupled channels/21 filter combinations with white LED) for trusted results
• User account management, locked workflow, and Pause features for greater control of experiment data
• Built-in software features to support compliance with 21 CFR Part 11 guidelines
• Secure cloud-based software provides increased accessibility and storage options
• Simplified instrument software with interactive touchscreen for greater ease of use
• 96-well 0.2mL format or 96-well 0.1mL format
Thermo Fisher Cloud
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Upload data from the instrument or upload your own files from anywhere you have web access. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use.
Downloads
>> QuantStudio 3/5 Design & Analysis Software | Release Notes | Example Data & Templates
>> QuantStudio 3/5 Firmware | Release Notes
Easy to use
The QuantStudio 5 Real-Time PCR System is compact in form, factory calibrated and is delivered in user-friendly packaging to enable fast and simple installation for immediate use. The interactive touchscreen provides a modern usability experience and the simplified instrument software/pre-optimized protocol templates allow users of any experience level to quickly set up and start a run.
Design and analysis software options:
Option 1: QuantStudio Design and Analysis cloud software
• Supports web browser-based system configuration with PC or MAC computer
• Streamlined software for improved usability and response time
• Enables secure access of your data when and where you want through the cloud
• No software to install, no licenses and no versions to update
Option 2: QuantStudio Design and Analysis desktop software
• Supports traditional co-located computer system configuration on Windows® 7 OS
• Streamlined software for improved usability and response time
• No licenses to purchase
Total control
With built-in software features to support compliance with 21 CFR Part 11 guidelines, user account management, locked workflow and Pause features, the QuantStudio 5 system offers users greater control of experiment data
Multiplex performance results that you can trust
Improved well-to-well and instrument-to-instrument data accuracy is achieved with OptiFlex technology. In the 96-well system this technology features 6 decoupled excitation and emission filter channels, for a total of 21 filter combinations, with white LED.
Sensitive data analysis
Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.
High application versatility
Simple and optimized protocols, reliable assays and reagents, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, gene regulation, and more.
Precision Plate Holder for 7000 System (Applied Biosystems™)
Precision plate holder for 7000 system.
For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostics procedures.
ViiA™ 7 Dx Instrument, 384 well, laptop (Applied Biosystems™)
The ViiA™ 7 Dx Instrument puts high-productivity qPCR at your fingertips. With the new OptiFlex™ optical system and 4 interchangeable block formats from 96-well to 384-well to Micro Fludic Array Card, the ViiA™ 7 Dx Instrument is available for in-vitro diagnostics use.
Key product features:
• Proven Applied Biosystems reliability and accuracy
• Using with Micro Fluidic Array Cards provides an integrated workflow and faster results
• Easy to use – intuitive software, responsive touch-screen, effortless block exchange without the need for any tools
Productivity
Ideal for performing medium- to high-throughput real-time PCR, the ViiA™ 7 Dx Instrument enhances your lab’s productivity:
• Quick block changes: Front access makes it easy to change thermal cycling block formats without having to move attached peripherals such as robots or computers
• Easy touch-screen interface: Instrument touch screen provides one-touch protocols for fast and easy assay setup for a broad range of applications
• Peace of mind: Data recovery capability for 100+ runs
Performance
The ViiA™ 7 Dx Instrument features the new OptiFlex™ System, which provides enhanced fluorescence detection:
• Maximum multiplexing: Six decoupled excitation and emission filter channels for the greatest number of dye combinations and maximum multiplexing capabilities
• Flexible data collection: Multiple ramp method detection formats provide more flexibility for collecting data during a ramp stage
• Precise quantification: Detect as small as 1.5–fold changes in target quantities in singleplex reactions
ViiA™ 7 Dx Software drives value in a number of ways, offering:
• Intuitive interface and innovative design
• Convenient walk-away operation
• Improved data analysis
• Fully compatible with high-throughput environments
Note: The ViiA™ 7 Dx Instrument and consumables are for distribution and use in specific European countries only. Not for use in the USA.
Key product features:
• Proven Applied Biosystems reliability and accuracy
• Using with Micro Fluidic Array Cards provides an integrated workflow and faster results
• Easy to use – intuitive software, responsive touch-screen, effortless block exchange without the need for any tools
Productivity
Ideal for performing medium- to high-throughput real-time PCR, the ViiA™ 7 Dx Instrument enhances your lab’s productivity:
• Quick block changes: Front access makes it easy to change thermal cycling block formats without having to move attached peripherals such as robots or computers
• Easy touch-screen interface: Instrument touch screen provides one-touch protocols for fast and easy assay setup for a broad range of applications
• Peace of mind: Data recovery capability for 100+ runs
Performance
The ViiA™ 7 Dx Instrument features the new OptiFlex™ System, which provides enhanced fluorescence detection:
• Maximum multiplexing: Six decoupled excitation and emission filter channels for the greatest number of dye combinations and maximum multiplexing capabilities
• Flexible data collection: Multiple ramp method detection formats provide more flexibility for collecting data during a ramp stage
• Precise quantification: Detect as small as 1.5–fold changes in target quantities in singleplex reactions
ViiA™ 7 Dx Software drives value in a number of ways, offering:
• Intuitive interface and innovative design
• Convenient walk-away operation
• Improved data analysis
• Fully compatible with high-throughput environments
Note: The ViiA™ 7 Dx Instrument and consumables are for distribution and use in specific European countries only. Not for use in the USA.
QuantStudio™ Dx Real-Time PCR Instrument with array card block (Applied Biosystems™)
The QuantStudio™ Dx Real-Time PCR Instrument with Array Card Block is a real-time PCR platform that delivers the proven performance, flexibility, expandability, and support needed to meet the evolving needs of the clinical laboratory today and tomorrow. The QuantStudio™ Dx Real-Time PCR Instrument with Array Card Block offers easy-to-change blocks that can also accommodate 96-well fast, 96-well standard, and 384-well plates, allowing you to select the format that is right for your throughput needs.
Built on a legacy of innovation and excellence in real-time PCR, the QuantStudio™ Dx instrument leverages the best of our portfolio of Applied Biosystems® real-time PCR systems, and refines a platform to specifically meet the needs of the diagnostic laboratory.
Results You Can Trust
The QuantStudio™ Dx Real-Time PCR Instrument’s proven performance, security, and reliability—combined with the outstanding sensitivity and specificity of the Quidel® Molecular Assays—help ensure the right answer is delivered the first time, every time.
Flexibility You Want, Security You Need
The QuantStudio™ Dx Real-Time PCR Instrument with Array Card Block is designed with easily interchangeable thermal cycling blocks to accommodate your laboratory’s varying needs for throughput, format type, and assay type. In addition to the array card format, the instrument also accepts 96-well fast, 96-well standard and 384-well card formats that can be used for laboratory-developed tests (LDTs) and future IVD assays. Additionally, the system can be operated in Test Development Mode, which enables clinical laboratories to develop their own assays or perform clinical research.
Efficient Workflows and Assays
A simplified instrument and assay workflow as well as intuitive software make the entire test easy and efficient, from processing to reporting. Plus, it frees up highly trained operators to perform more complex tasks.
QuantStudio™ Dx Software
• Track reagent and patient information for quality control purposes
• Enter reagent information by barcode and upload patient information from Excel files to minimize data entry errors
• Print calibration and maintenance reports for your instrument records
• Included SAE module assists with Security, Auditing, and Electronic signature of records for full data traceability as part of 21 CRF Part 11 compliance
For In Vitro Diagnostic Use. The QuantStudio™ Dx Real-Time PCR Instrument meets the requirements of the in vitro Diagnostic Medical Devices Directive (98/79/EC). The CE IVD-registered QuantStudio™ Dx Real-Time PCR Instrument is for distribution and use in specific countries only. Not available for sale in the United States.
Built on a legacy of innovation and excellence in real-time PCR, the QuantStudio™ Dx instrument leverages the best of our portfolio of Applied Biosystems® real-time PCR systems, and refines a platform to specifically meet the needs of the diagnostic laboratory.
Results You Can Trust
The QuantStudio™ Dx Real-Time PCR Instrument’s proven performance, security, and reliability—combined with the outstanding sensitivity and specificity of the Quidel® Molecular Assays—help ensure the right answer is delivered the first time, every time.
Flexibility You Want, Security You Need
The QuantStudio™ Dx Real-Time PCR Instrument with Array Card Block is designed with easily interchangeable thermal cycling blocks to accommodate your laboratory’s varying needs for throughput, format type, and assay type. In addition to the array card format, the instrument also accepts 96-well fast, 96-well standard and 384-well card formats that can be used for laboratory-developed tests (LDTs) and future IVD assays. Additionally, the system can be operated in Test Development Mode, which enables clinical laboratories to develop their own assays or perform clinical research.
Efficient Workflows and Assays
A simplified instrument and assay workflow as well as intuitive software make the entire test easy and efficient, from processing to reporting. Plus, it frees up highly trained operators to perform more complex tasks.
QuantStudio™ Dx Software
• Track reagent and patient information for quality control purposes
• Enter reagent information by barcode and upload patient information from Excel files to minimize data entry errors
• Print calibration and maintenance reports for your instrument records
• Included SAE module assists with Security, Auditing, and Electronic signature of records for full data traceability as part of 21 CRF Part 11 compliance
For In Vitro Diagnostic Use. The QuantStudio™ Dx Real-Time PCR Instrument meets the requirements of the in vitro Diagnostic Medical Devices Directive (98/79/EC). The CE IVD-registered QuantStudio™ Dx Real-Time PCR Instrument is for distribution and use in specific countries only. Not available for sale in the United States.
Pharmaceutical Analytics QuantStudio™ 5 Real-Time PCR System, 384-well, laptop (Applied Biosystems™)
The Applied Biosystems™ QuantStudio™ 5 Real-Time PCR System for Pharmaceutical Analytics is designed for users who need superior performance, maximum dye versatility, and security options in a real-time PCR system that is affordable and easy to use. The QuantStudio 5 pharmaceutical analytics system is used together with specialized sample preparation and quantitation kits for host cell protein, host cell DNA, and Protein A impurity testing of biological products produced in cell culture.The optimized Design and Analysis software is ideal for both first-time and experienced users. When connected to the Thermo Fisher Cloud, the QuantStudio 5 system provides access to your data wherever and whenever you want. Using proven OptiFlex® technology (featuring 6 decoupled channels and white LED) and featuring six independent Veriflex® temperature zones, the QuantStudio 5 96-well system enables improved data accuracy and sensitivity for a broad range of genomic applications. The QuantStudio 5 system also offers the built-in software features of electronic record security and prevention of unauthorized instrument access to assist with 21 CFR Part 11 compliance.
Features of the QuantStudio 5 system with 96-well block include:
• Proven OptiFlex technology (6 decoupled channels/21 filter combinations with white LED) for trusted results
• User account management, locked workflow, and Pause features for greater control of experiment data
• Built-in software features to support compliance with 21 CFR Part 11 guidelines
• Secure cloud-based software provides increased accessibility and storage options
• Simplified instrument software with interactive touchscreen for greater ease of use
• 96-well 0.2mL format or 96-well 0.1mL format
Thermo Fisher Cloud
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Upload data from the instrument or upload your own files from anywhere you have web access. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use.
Downloads
>> QuantStudio 3/5 Design & Analysis Software | Release Notes | Example Data & Templates
>> QuantStudio 3/5 Firmware | Release Notes
Easy to use
The QuantStudio 5 Real-Time PCR System is compact in form, factory calibrated and is delivered in user-friendly packaging to enable fast and simple installation for immediate use. The interactive touchscreen provides a modern usability experience and the simplified instrument software/pre-optimized protocol templates allow users of any experience level to quickly set up and start a run.
Design and analysis software options:
Option 1: QuantStudio Design and Analysis cloud software
• Supports web browser-based system configuration with PC or MAC computer
• Streamlined software for improved usability and response time
• Enables secure access of your data when and where you want through the cloud
• No software to install, no licenses and no versions to update
Option 2: QuantStudio Design and Analysis desktop software
• Supports traditional co-located computer system configuration on Windows® 7 OS
• Streamlined software for improved usability and response time
• No licenses to purchase
Total control
With built-in software features to support compliance with 21 CFR Part 11 guidelines, user account management, locked workflow and Pause features, the QuantStudio 5 system offers users greater control of experiment data
Multiplex performance results that you can trust
Improved well-to-well and instrument-to-instrument data accuracy is achieved with OptiFlex technology. In the 96-well system this technology features 6 decoupled excitation and emission filter channels, for a total of 21 filter combinations, with white LED.
Sensitive data analysis
Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.
High application versatility
Simple and optimized protocols, reliable assays and reagents, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, gene regulation, and more.
Features of the QuantStudio 5 system with 96-well block include:
• Proven OptiFlex technology (6 decoupled channels/21 filter combinations with white LED) for trusted results
• User account management, locked workflow, and Pause features for greater control of experiment data
• Built-in software features to support compliance with 21 CFR Part 11 guidelines
• Secure cloud-based software provides increased accessibility and storage options
• Simplified instrument software with interactive touchscreen for greater ease of use
• 96-well 0.2mL format or 96-well 0.1mL format
Thermo Fisher Cloud
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Upload data from the instrument or upload your own files from anywhere you have web access. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use.
Downloads
>> QuantStudio 3/5 Design & Analysis Software | Release Notes | Example Data & Templates
>> QuantStudio 3/5 Firmware | Release Notes
Easy to use
The QuantStudio 5 Real-Time PCR System is compact in form, factory calibrated and is delivered in user-friendly packaging to enable fast and simple installation for immediate use. The interactive touchscreen provides a modern usability experience and the simplified instrument software/pre-optimized protocol templates allow users of any experience level to quickly set up and start a run.
Design and analysis software options:
Option 1: QuantStudio Design and Analysis cloud software
• Supports web browser-based system configuration with PC or MAC computer
• Streamlined software for improved usability and response time
• Enables secure access of your data when and where you want through the cloud
• No software to install, no licenses and no versions to update
Option 2: QuantStudio Design and Analysis desktop software
• Supports traditional co-located computer system configuration on Windows® 7 OS
• Streamlined software for improved usability and response time
• No licenses to purchase
Total control
With built-in software features to support compliance with 21 CFR Part 11 guidelines, user account management, locked workflow and Pause features, the QuantStudio 5 system offers users greater control of experiment data
Multiplex performance results that you can trust
Improved well-to-well and instrument-to-instrument data accuracy is achieved with OptiFlex technology. In the 96-well system this technology features 6 decoupled excitation and emission filter channels, for a total of 21 filter combinations, with white LED.
Sensitive data analysis
Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.
High application versatility
Simple and optimized protocols, reliable assays and reagents, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, gene regulation, and more.
ViiA™ 7 Dx Instrument, array card, laptop (Applied Biosystems™)
The ViiA™ 7 Dx Instrument puts high-productivity qPCR at your fingertips. With the new OptiFlex™ optical system and 4 interchangeable block formats from 96-well to 384-well to Micro Fludic Array Card, the ViiA™ 7 Dx Instrument is available for in-vitro diagnostics use.
Key product features
• Proven Applied Biosystems reliability and accuracy
• Using with Micro Fluidic Array Cards provides an integrated workflow and faster results
• Easy to use – intuitive software, responsive touch-screen, effortless block exchange without the need for any tools
Productivity
Ideal for performing medium- to high-throughput real-time PCR, the ViiA™ 7 Dx Instrument enhances your lab’s productivity:
• Quick block changes: Front access makes it easy to change thermal cycling block formats without having to move attached peripherals such as robots or computers
• Easy touch-screen interface: Instrument touch screen provides one-touch protocols for fast and easy assay setup for a broad range of applications
• Peace of mind: Data recovery capability for 100+ runs
Performance
The ViiA™ 7 Dx Instrument features the new OptiFlex™ System, which provides enhanced fluorescence detection:
• Maximum multiplexing: Six decoupled excitation and emission filter channels for the greatest number of dye combinations and maximum multiplexing capabilities
• Flexible data collection: Multiple ramp method detection formats provide more flexibility for collecting data during a ramp stage
• Precise quantification: Detect as small as 1.5–fold changes in target quantities in singleplex reactions
ViiA™ 7 Dx Software drives value in a number of ways, offering:
• Intuitive interface and innovative design
• Convenient walk-away operation
• Improved data analysis
• Fully compatible with high-throughput environments
Note: The ViiA™ 7 Dx Instrument and consumables are for distribution and use in specific European countries only. Not for use in the USA.
Key product features
• Proven Applied Biosystems reliability and accuracy
• Using with Micro Fluidic Array Cards provides an integrated workflow and faster results
• Easy to use – intuitive software, responsive touch-screen, effortless block exchange without the need for any tools
Productivity
Ideal for performing medium- to high-throughput real-time PCR, the ViiA™ 7 Dx Instrument enhances your lab’s productivity:
• Quick block changes: Front access makes it easy to change thermal cycling block formats without having to move attached peripherals such as robots or computers
• Easy touch-screen interface: Instrument touch screen provides one-touch protocols for fast and easy assay setup for a broad range of applications
• Peace of mind: Data recovery capability for 100+ runs
Performance
The ViiA™ 7 Dx Instrument features the new OptiFlex™ System, which provides enhanced fluorescence detection:
• Maximum multiplexing: Six decoupled excitation and emission filter channels for the greatest number of dye combinations and maximum multiplexing capabilities
• Flexible data collection: Multiple ramp method detection formats provide more flexibility for collecting data during a ramp stage
• Precise quantification: Detect as small as 1.5–fold changes in target quantities in singleplex reactions
ViiA™ 7 Dx Software drives value in a number of ways, offering:
• Intuitive interface and innovative design
• Convenient walk-away operation
• Improved data analysis
• Fully compatible with high-throughput environments
Note: The ViiA™ 7 Dx Instrument and consumables are for distribution and use in specific European countries only. Not for use in the USA.
ViiA™ 7 Dx Instrument, Fast 96 well, desktop (Applied Biosystems™)
The ViiA™ 7 Dx Instrument puts high-productivity qPCR at your fingertips. With the new OptiFlex™ optical system and 4 interchangeable block formats from 96-well to 384-well to Micro Fludic Array Card, the ViiA™ 7 Dx Instrument is available for in-vitro diagnostics use.
Key product features:
• Proven Applied Biosystems reliability and accuracy
• Using with Micro Fluidic Array Cards provides an integrated workflow and faster results
• Easy to use – intuitive software, responsive touch-screen, effortless block exchange without the need for any tools
Productivity
Ideal for performing medium- to high-throughput real-time PCR, the ViiA™ 7 Dx Instrument enhances your lab’s productivity:
• Quick block changes: Front access makes it easy to change thermal cycling block formats without having to move attached peripherals such as robots or computers
• Easy touch-screen interface: Instrument touch screen provides one-touch protocols for fast and easy assay setup for a broad range of applications
• Peace of mind: Data recovery capability for 100+ runs
Performance
The ViiA™ 7 Dx Instrument features the new OptiFlex™ System, which provides enhanced fluorescence detection:
• Maximum multiplexing: Six decoupled excitation and emission filter channels for the greatest number of dye combinations and maximum multiplexing capabilities
• Flexible data collection: Multiple ramp method detection formats provide more flexibility for collecting data during a ramp stage
• Precise quantification: Detect as small as 1.5–fold changes in target quantities in singleplex reactions
ViiA™ 7 Dx Software drives value in a number of ways, offering:
• Intuitive interface and innovative design
• Convenient walk-away operation
• Improved data analysis
• Fully compatible with high-throughput environments
Note: The ViiA™ 7 Dx Instrument and consumables are for distribution and use in specific European countries only. Not for use in the USA.
Key product features:
• Proven Applied Biosystems reliability and accuracy
• Using with Micro Fluidic Array Cards provides an integrated workflow and faster results
• Easy to use – intuitive software, responsive touch-screen, effortless block exchange without the need for any tools
Productivity
Ideal for performing medium- to high-throughput real-time PCR, the ViiA™ 7 Dx Instrument enhances your lab’s productivity:
• Quick block changes: Front access makes it easy to change thermal cycling block formats without having to move attached peripherals such as robots or computers
• Easy touch-screen interface: Instrument touch screen provides one-touch protocols for fast and easy assay setup for a broad range of applications
• Peace of mind: Data recovery capability for 100+ runs
Performance
The ViiA™ 7 Dx Instrument features the new OptiFlex™ System, which provides enhanced fluorescence detection:
• Maximum multiplexing: Six decoupled excitation and emission filter channels for the greatest number of dye combinations and maximum multiplexing capabilities
• Flexible data collection: Multiple ramp method detection formats provide more flexibility for collecting data during a ramp stage
• Precise quantification: Detect as small as 1.5–fold changes in target quantities in singleplex reactions
ViiA™ 7 Dx Software drives value in a number of ways, offering:
• Intuitive interface and innovative design
• Convenient walk-away operation
• Improved data analysis
• Fully compatible with high-throughput environments
Note: The ViiA™ 7 Dx Instrument and consumables are for distribution and use in specific European countries only. Not for use in the USA.
QuantStudio™ 3D Digital PCR Chip Loader (Applied Biosystems™)
The Applied Biosystems® QuantStudio™ 3D Digital PCR Chip Loader is part of the QuantStudio™ 3D Digital PCR System, a small-footprint digital PCR platform with high accuracy, sensitivity, and precision that is affordable and simple to use at any experience level. The chip loader enables operator consistency & data reproducibility during chip loading and is user-installed. A chip loader is included with the QuantStudio™ 3D Digital PCR System Premium Package, but not included with the QuantStudio™ 3D Digital PCR System Value Package.
Système de PCR en temps réel SureTect™ (Thermo Scientific™)
Détectez les micro-organismes rapidement et précisément dans une vaste gamme d’échantillons environnementaux et alimentaires grâce au système Thermo Scientific™ SureTect™.
QuantStudio™ 12K Flex Real-Time PCR System, Fast 96-well block, laptop (Applied Biosystems™)
The QuantStudio™ 12K Flex system is a highly flexible, comprehensive real-time PCR platform that leverages the best of Applied Biosystems™ real-time technology in a single instrument. The QuantStudio 12K Flex Real-Time PCR System includes easy-to-change blocks that can accommodate OpenArray™ plates, TaqMan™ array cards, 384-well, 96-well, and FAST 96-well plates allowing you to select the format that is right for your project whether it's now or in the future, for maximum flexibility and consistency in your research.
Thermo Fisher Cloud
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use. These software tools are compatible with Mac or PC as well as most Applied Biosystems instrument file types.
The QuantStudio 12K Flex System's intuitive software, easy touch screen setup, automation integration, effortless block exchange, and easy-to- use OpenArray components are designed to let you get started right away and reduce hands on time. Plan for the future and save time, money, and space in your lab with the only all-in-one qPCR instrument enabling a broad range of applications with maximum throughput.
Flexibility & Scalability
• Includes five interchangeable blocks: The QuantStudio 12K Flex blocks accomodate 96-well, FAST 96-well, 384-well, TaqMan array cards, and OpenArray plates, for effortless scaling from 1 to 12,000 data points in a single run. Increase throughput as you dive deeper into your research, using just one instrument. As you begin your project, start with 96-well format experiments and then scale up to a higher throughput to get the statistically significant answers you need. Additionally, you can easily validate and screen your targets of interest with a selected panel of genes using novel formats such as the TaqMan array cards and OpenArray plates.
• Compatible with a full range of TaqMan assays: The QuantStudio 12K Flex System is optimized to enable clear, clean data for all TaqMan assays that might be used across a broad range of applications, including gene expression, microRNA and small noncoding RNAs, SNP genotyping, protein analysis, protein thermal shift, high resolution melt, copy number variation, and pathogen detection.
• Provides a seamless switch from qPCR to digital PCR: Increase the precision and sensitivity of your nucleic acid experiment by switching from qPCR to digital PCR mode using QuantStudio Digital PCR kits and DigitalSuite™ Software with the QuantStudio OpenArray block.
• Delivers peace of mind: Leave early on a Friday and monitor runs from home, knowing that the system can safely store over 100 runs.
Maximum Throughput
• Enhanced OptiFlex™ System: Building upon Applied Biosystems technology, the Enhanced OptiFlex System provides enhanced fluorescence detection for the accurate and sensitive data analysis necessary to collect 12,000 data points in a single run.
• Unprecedented coverage and sample throughput: The OpenArray format accelerates genomic validation and screening, driving over 150,000 data points per work day, enabling most projects to be completed in days instead of weeks.
• Simple OpenArray workflow: QuantStudio Open Array plates arrive in a case to facilitate ease of handling and ensure optimal data quality upon loading of samples using the Accufill™ System. Easily load four arrays into the QuantStudio 12K Flex instrument to achieve over 12,000 data points. Integrated sample tracking software lets you easily track your samples throughout the course of your project.
• Integrated analysis and quality systems: New intuitive touch-screen interface and integrated calendar feature allows for scheduling time of use on the instrument. Additional analysis software such as DigitalSuite v1.0, ExpressionSuite v1.0, and Genotyper v1.1 is available to help you analyze hundreds of targets and samples and achieve the statistical power you need.
QuantStudio 12K Flex Software
• Intuitive interface and innovative design for novice and experienced users
• Convenient walk-away operation
• Improved data analysis across block types
• Fully compatible with high-throughput environments
Expandable Software Architecture
Optional add-ons are available to upgrade the existing software:
• 21 CFR Part 11 compliance: This optional SAE module assists with Security, Auditing, and Electronic signature of records for full data traceability.
• High Resolution Melt (HRM): Using the built-in MeltDoctor™ protocols and calibrations, you can spend less time optimizing the run and more time customizing your results. Define the number of variants you want to see by quickly changing analysis settings.
• DigitalSuite Software v1.0: For OpenArray block users needing sensitivity for rare targets and precision to measure small differences in target abundance.
• ExpressionSuite Software v1.0: Stand-alone gene expression software with enhanced analysis tools that can analyze over 200 384-well plates or 10 OpenArray plates.
Thermo Fisher Cloud
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use. These software tools are compatible with Mac or PC as well as most Applied Biosystems instrument file types.
The QuantStudio 12K Flex System's intuitive software, easy touch screen setup, automation integration, effortless block exchange, and easy-to- use OpenArray components are designed to let you get started right away and reduce hands on time. Plan for the future and save time, money, and space in your lab with the only all-in-one qPCR instrument enabling a broad range of applications with maximum throughput.
Flexibility & Scalability
• Includes five interchangeable blocks: The QuantStudio 12K Flex blocks accomodate 96-well, FAST 96-well, 384-well, TaqMan array cards, and OpenArray plates, for effortless scaling from 1 to 12,000 data points in a single run. Increase throughput as you dive deeper into your research, using just one instrument. As you begin your project, start with 96-well format experiments and then scale up to a higher throughput to get the statistically significant answers you need. Additionally, you can easily validate and screen your targets of interest with a selected panel of genes using novel formats such as the TaqMan array cards and OpenArray plates.
• Compatible with a full range of TaqMan assays: The QuantStudio 12K Flex System is optimized to enable clear, clean data for all TaqMan assays that might be used across a broad range of applications, including gene expression, microRNA and small noncoding RNAs, SNP genotyping, protein analysis, protein thermal shift, high resolution melt, copy number variation, and pathogen detection.
• Provides a seamless switch from qPCR to digital PCR: Increase the precision and sensitivity of your nucleic acid experiment by switching from qPCR to digital PCR mode using QuantStudio Digital PCR kits and DigitalSuite™ Software with the QuantStudio OpenArray block.
• Delivers peace of mind: Leave early on a Friday and monitor runs from home, knowing that the system can safely store over 100 runs.
Maximum Throughput
• Enhanced OptiFlex™ System: Building upon Applied Biosystems technology, the Enhanced OptiFlex System provides enhanced fluorescence detection for the accurate and sensitive data analysis necessary to collect 12,000 data points in a single run.
• Unprecedented coverage and sample throughput: The OpenArray format accelerates genomic validation and screening, driving over 150,000 data points per work day, enabling most projects to be completed in days instead of weeks.
• Simple OpenArray workflow: QuantStudio Open Array plates arrive in a case to facilitate ease of handling and ensure optimal data quality upon loading of samples using the Accufill™ System. Easily load four arrays into the QuantStudio 12K Flex instrument to achieve over 12,000 data points. Integrated sample tracking software lets you easily track your samples throughout the course of your project.
• Integrated analysis and quality systems: New intuitive touch-screen interface and integrated calendar feature allows for scheduling time of use on the instrument. Additional analysis software such as DigitalSuite v1.0, ExpressionSuite v1.0, and Genotyper v1.1 is available to help you analyze hundreds of targets and samples and achieve the statistical power you need.
QuantStudio 12K Flex Software
• Intuitive interface and innovative design for novice and experienced users
• Convenient walk-away operation
• Improved data analysis across block types
• Fully compatible with high-throughput environments
Expandable Software Architecture
Optional add-ons are available to upgrade the existing software:
• 21 CFR Part 11 compliance: This optional SAE module assists with Security, Auditing, and Electronic signature of records for full data traceability.
• High Resolution Melt (HRM): Using the built-in MeltDoctor™ protocols and calibrations, you can spend less time optimizing the run and more time customizing your results. Define the number of variants you want to see by quickly changing analysis settings.
• DigitalSuite Software v1.0: For OpenArray block users needing sensitivity for rare targets and precision to measure small differences in target abundance.
• ExpressionSuite Software v1.0: Stand-alone gene expression software with enhanced analysis tools that can analyze over 200 384-well plates or 10 OpenArray plates.
StepOnePlus™ Real-Time PCR System, laptop (Applied Biosystems™)
The StepOnePlus™ Real-Time PCR System is a 96-well Real-Time PCR instrument perfect for both first-time and experienced users. The StepOnePlus™ Real-Time PCR System can be setup in a variety of configurations and comes ready to use, out of the box, with intuitive data analysis and instrument control software. Utilizing robust LED based 4-color optical recording, the StepOnePlus™ Real-Time PCR System is designed to deliver precise, quantitative Real-Time PCR results for a variety of genomic research applications. We supply a Dell™ Laptop with the StepOnePlus™ Real-Time PCR System.
Features of the StepOnePlus™ Real-Time PCR System include:
• Advanced software and instrumentation for performing a wide array of genomic assays
• Sensitive 4-color optical LED recording system
• Intuitive and robust software perfect for both first-time and advanced users
• Simple and flexible instrument set-up and usage
New StepOne™ Software
The StepOne™ Software included with the StepOnePlus™ Real-Time PCR System runs on the Windows® XP operating system and provides instrument control, data collection, and data analysis capabilities (analysis software is also compatible with Windows® 7). This latest version includes capabilities to collect melt curve data for High Resolution Melt (HRM) applications and the option to export in Real-Time PCR Data Markup Language (RDML) for compatibility with MIQE guidelines.
Rapid and Simple Assay Set-Up
This remarkably simple Real-Time PCR system is designed with an LCD touchscreen and a user-friendly interface that brings the power of genetic studies to researchers new to Real-Time PCR (Fig 1). The intuitive software and protocol wizards help guide new users through their Real-Time PCR experiments. The StepOnePlus™ Real-Time PCR System also includes a quick-start setup so that you start a run immediately and enter plate information at a later time.
Sensitive LED-Based 4 Color Fluorescence Reading
The StepOnePlus™ Real-Time PCR System utilizes a long-life LED-based optical system that can record fluorescence from FAM™/SYBR® Green, VIC®/JOE™, NED™/TAMRA™, and ROX™ dyes. This cost-effective, 4-color, 96-well system delivers precise, quantitative Real-Time PCR results and saves data from all filters in every run without depending on a computer or plate setup. It can discriminate between 2 populations of 5,000 and 10,000 template copies of a TaqMan® assay with 99.7% confidence.
Compatible with Many Genomic Analysis Techniques
Perform a variety of standard and demanding genetic analysis research techniques on one instrument using the user friendly StepOnePlus™ Real-Time PCR System and StepOne™ Software.
The StepOnePlus™ System supports many Real-Time PCR applications, including the following:
• SNP Genotyping
• Gene Expression Analysis
• MicroRNA Expression
• Protein Expression
• Translocation Analysis
• Gene Detection
• Viral Load Analysis
The included StepOne™ Software supports a variety of analysis methods, including the following:
• Standard Curve (absolute quantitation)
• Relative Standard Curve
• Comparative Ct (ΔΔCt) (relative quantitation)
• Genotyping and Presence/Absence
• Melt Curve Analysis (as a standalone application)
• High Resolution Melting (as a standalone application)
Versatile Instrument Configuration Options
The ultra-compact footprint of a StepOnePlus™ System can be installed in multiple distinct configurations, providing unmatched flexibility and convenience that can allow a fit in any laboratory (Fig 2). The StepOnePlus™ Real-Time PCR System can be used via a PC, with a PC connected to a Local Area Network (LAN), or as a stand-alone instrument (PC-free). Each StepOnePlus™ Real-Time PCR System is factory-calibrated for optical and thermal accuracy, so simply remarkable Real-Time PCR results can be obtained right out of the box.
Energy Efficiency and Space Saving
The StepOnePlus™ Real-Time PCR System draws 38% less energy to process one sample plate (when the instrument is in a heated state), compared to the Bio-Rad CFX96™ Real-Time PCR Detection System. The StepOnePlus™ Real-Time PCR System also has advanced temperature control through use of VeriFlex™ Blocks technology and has a footprint nearly 15% smaller than Bio-Rad CFX96™, which in today’s crowded laboratories helps you use your laboratory space even more efficiently. You also leave a smaller environmental footprint on the Earth.
Real-Time Data Monitoring, Dissemination, and Storage
The system measures amplification as it occurs, allowing you to monitor the progress of your experiment cycle by cycle, either on the machine or remotely. Your data is stored on the instrument itself, and can be viewed and stored automatically via remote access, or transferred via email or a USB flash drive. Data can also be conveniently exported as PowerPoint®, Excel®, and graphical image files.
Expanded Gene Expression Capabilities
The advanced software provided with the StepOnePlus™ Real-Time PCR System now includes the powerful Gene Expression Study Package. This software package allows for greater flexibility and accuracy in your gene expression assays through:
• Analysis of an unlimited number of plates in one study
• Sorting of data by biological or technical replicate group
• Use of multiple endogenous controls
• Assay efficiency correction
Advanced High Resolution Melting Capability
Now with High Resolution Melt Software v3.0, you can perform post-PCR sequence variation analysis with the StepOnePlus™ Real-Time PCR System. This separately purchased software simplifies set-up by maintaining assay specific settings and accepts pasted plate layout information directly from Excel. HRM Software v3.0 also has an improved clustering algorithm for increased sensitivity and accuracy, and the ability to conduct separate analyses of multiple assays run on a single plate.
Features of the StepOnePlus™ Real-Time PCR System include:
• Advanced software and instrumentation for performing a wide array of genomic assays
• Sensitive 4-color optical LED recording system
• Intuitive and robust software perfect for both first-time and advanced users
• Simple and flexible instrument set-up and usage
New StepOne™ Software
The StepOne™ Software included with the StepOnePlus™ Real-Time PCR System runs on the Windows® XP operating system and provides instrument control, data collection, and data analysis capabilities (analysis software is also compatible with Windows® 7). This latest version includes capabilities to collect melt curve data for High Resolution Melt (HRM) applications and the option to export in Real-Time PCR Data Markup Language (RDML) for compatibility with MIQE guidelines.
Rapid and Simple Assay Set-Up
This remarkably simple Real-Time PCR system is designed with an LCD touchscreen and a user-friendly interface that brings the power of genetic studies to researchers new to Real-Time PCR (Fig 1). The intuitive software and protocol wizards help guide new users through their Real-Time PCR experiments. The StepOnePlus™ Real-Time PCR System also includes a quick-start setup so that you start a run immediately and enter plate information at a later time.
Sensitive LED-Based 4 Color Fluorescence Reading
The StepOnePlus™ Real-Time PCR System utilizes a long-life LED-based optical system that can record fluorescence from FAM™/SYBR® Green, VIC®/JOE™, NED™/TAMRA™, and ROX™ dyes. This cost-effective, 4-color, 96-well system delivers precise, quantitative Real-Time PCR results and saves data from all filters in every run without depending on a computer or plate setup. It can discriminate between 2 populations of 5,000 and 10,000 template copies of a TaqMan® assay with 99.7% confidence.
Compatible with Many Genomic Analysis Techniques
Perform a variety of standard and demanding genetic analysis research techniques on one instrument using the user friendly StepOnePlus™ Real-Time PCR System and StepOne™ Software.
The StepOnePlus™ System supports many Real-Time PCR applications, including the following:
• SNP Genotyping
• Gene Expression Analysis
• MicroRNA Expression
• Protein Expression
• Translocation Analysis
• Gene Detection
• Viral Load Analysis
The included StepOne™ Software supports a variety of analysis methods, including the following:
• Standard Curve (absolute quantitation)
• Relative Standard Curve
• Comparative Ct (ΔΔCt) (relative quantitation)
• Genotyping and Presence/Absence
• Melt Curve Analysis (as a standalone application)
• High Resolution Melting (as a standalone application)
Versatile Instrument Configuration Options
The ultra-compact footprint of a StepOnePlus™ System can be installed in multiple distinct configurations, providing unmatched flexibility and convenience that can allow a fit in any laboratory (Fig 2). The StepOnePlus™ Real-Time PCR System can be used via a PC, with a PC connected to a Local Area Network (LAN), or as a stand-alone instrument (PC-free). Each StepOnePlus™ Real-Time PCR System is factory-calibrated for optical and thermal accuracy, so simply remarkable Real-Time PCR results can be obtained right out of the box.
Energy Efficiency and Space Saving
The StepOnePlus™ Real-Time PCR System draws 38% less energy to process one sample plate (when the instrument is in a heated state), compared to the Bio-Rad CFX96™ Real-Time PCR Detection System. The StepOnePlus™ Real-Time PCR System also has advanced temperature control through use of VeriFlex™ Blocks technology and has a footprint nearly 15% smaller than Bio-Rad CFX96™, which in today’s crowded laboratories helps you use your laboratory space even more efficiently. You also leave a smaller environmental footprint on the Earth.
Real-Time Data Monitoring, Dissemination, and Storage
The system measures amplification as it occurs, allowing you to monitor the progress of your experiment cycle by cycle, either on the machine or remotely. Your data is stored on the instrument itself, and can be viewed and stored automatically via remote access, or transferred via email or a USB flash drive. Data can also be conveniently exported as PowerPoint®, Excel®, and graphical image files.
Expanded Gene Expression Capabilities
The advanced software provided with the StepOnePlus™ Real-Time PCR System now includes the powerful Gene Expression Study Package. This software package allows for greater flexibility and accuracy in your gene expression assays through:
• Analysis of an unlimited number of plates in one study
• Sorting of data by biological or technical replicate group
• Use of multiple endogenous controls
• Assay efficiency correction
Advanced High Resolution Melting Capability
Now with High Resolution Melt Software v3.0, you can perform post-PCR sequence variation analysis with the StepOnePlus™ Real-Time PCR System. This separately purchased software simplifies set-up by maintaining assay specific settings and accepts pasted plate layout information directly from Excel. HRM Software v3.0 also has an improved clustering algorithm for increased sensitivity and accuracy, and the ability to conduct separate analyses of multiple assays run on a single plate.
