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This is a conditioning reagent container designed for use with the 3500 Dx⁄3500xL Dx Genetic Analyzer.

The 3500 Dx series conditioning reagent, anode buffer, cathode buffer and septa cathode buffer are required for instrument operation.

The 3500 Dx series conditioning reagent is available as a ready-to-use pouch, and is used for priming the polymer pump, washing the polymer pump between polymer type changes, and during instrument shut down. The pouch has adequate volume for a one-time use. The top part of the pouch fitment is sealed with a plastic film, which should be removed prior to installation on to the instrument.

This product is intended for in-vitro diagnostic use on the Applied Biosystems 3500 Dx Series Genetic Analyzers and is not validated for use with other systems or applications. The 3500 Dx Series Genetic Analyzer is to be used by trained operators in the analysis of human DNA or RNA, for the detection of genetic changes that may lead to disease presence or susceptibility. Off-label uses such as fragment analysis are for Research Use Only. Refer to the Applied Biosystems 3500 Dx⁄3500xL Dx Genetic Analyzer User Guide for further details.

The Ion Total RNA Library Protocol Card for AB Library Builder™System is required for running the Ion Total RNA-Seq Kit for AB for Library Builder™System. This protocol card contains the firmware needed to easily automate the creation of up to 13 whole transcriptome or small RNA (miRNA) libraries upstream of semiconductor sequencing on either the Ion PGM™ or Ion Proton™ platforms.

The Ion Total RNA Library Protocol Card for AB Library Builder™System:
• Enables whole transcriptome or small RNA (miRNA) library construction on the AB Library Builder™System
• Eliminates the need for instrument programming prior to use

This automated RNA library preparation solution eliminates the labor intensive steps typically associated with the manual RNA-Seq library protocols. The protocol card also offers the:
• Convenience of having instrument software for automating the preparation of two different RNA-Seq library types on the AB Library Builder™System on an easy-to-install firmware card
• Simplicity of running multiple protocols without the need for instrument programming or time-consuming instrument training

A Single-Day Workflow: The Next Stage in a Sequencing Revolution
With the ability to prepare up to 13 libraries in approximately 3 hours, the Ion Total RNA-Seq Protocol Card for the AB Library Builder™System, in combination with the Ion Total RNA-Seq kit for the AB Library Builder™System, represents a step towards automating the entire Ion RNA sequencing workflow, making analysis of both whole transcriptome and small RNA more convenient than ever before. This protocol card makes implementing these automated workflows simple and easy, for a transition from manual to automated library construction that is rapid and painless.

This is an accessory for use with the SOLiD™ Analyzer.

For Research Use Only. Not for use in diagnostics procedures.

The 5500 W FlowChip V2 is a microfluidic glass device with primers on the surface that enable amplification of library template onto the FlowChip for ligation sequencing on the 5500 W Series genetic analyzers. The 5500 W FlowChip V2 is specially designed with precisely cut lanes. Individual library samples can be deposited for amplification in each lane of the FlowChip. Sequencing reagents are delivered to each lane separately, enabling consumption of sequencing reagents for just the number of lanes used for sequencing.

The 5500 W FlowChip V2 is notched to set the orientation. Each 5500 W FlowChip V2 kit contains one 5500 W FlowChip V2 and a deposition cassette that aids in depositing sample libraries. The 5500 W FlowChip Prep Pack (available separately) contains reagents for depositing library template onto the 5500 W FlowChip V2.

This is an optical upgrade kit for the 5500xl Genetic Analyzer for conversion to a 5500xl-W system.

Our new 5500xl-W Genetic Analyzer helps achieve optimal productivity with a faster workflow, two flexible FlowChips, intuitive user workflows, and project scalability. Faster turn-around times are achieved through the introduction of a non-bead based workflow and increased throughput with a combination of higher colony density and improved optics. The 5500xl-W Genetic Analyzer offers these improvements while maintaining the benefits of the 5500 and 5500xl systems: accuracy, sensitivity, and cost effectiveness. With the 5500xl-W Genetic Analyzer, you are empowered to discover rare genetic events or sub-populations of somatic mutations at an unprecedented pace.

Key Benefits:
• Sequencing right here, right now
• Cost-effective sequencing runs with individually configurable lanes
• Superior low-frequency variant detection for whole-exome sequencing or targeted resequencing
• Reproducibility, reliability, and quality for your RNA applications
• Confidence and uniformity in multiplexing with up to 96 barcodes
• Optimal analysis solutions for streamlined productivity and freedom to operate
• New non-bead based workflow for improved project turnaround and efficiency

When Coverage is Not Enough
Additional sequence coverage fails to compensate for poor accuracy in the detection of rare variants that may hold the key to a better understanding cancer progression, disease penetrance, or drug resistance. With a system accuracy of up to 99.999%, the 5500xl-W Genetic Analyzer enables you to perform 25% less sequencing to detect rare variants than a next-generation sequencing platform with 99.99% system accuracy in order to detect somatic variant present at 1% (Figure 2). The industry-leading accuracy of the 5500xl-W Genetic Analyzer enables detection of significant biological variation for applications like whole genome resequencing, targeted resequencing, and whole transcriptome analysis.

When Flexibility, Speed, and Cost are Crucial
Multidisciplinary translational research programs often require processing of multiple samples across multiple applications like whole exome, RNA-seq, and structural variation. The 5500xl-W Genetic Analyzer provides you the flexibility to configure your sequencing runs to your project and throughput needs (Figure 3). The system’s two configurable microfluidic FlowChips process 6–12 independent samples, and the intelligent barcoding kits multiplex up to hundreds of samples in a single run. Pay-Per-Lane Sequencing (PPL-Seq™) and independent run lanes tailor the system to any project scale. The new non-bead based workflow shaves 1–2 days off project workflows for faster project data delivery. Increased colony density coupled with improved optics provides more data from each lane. With the 5500xl-W Genetic Analyzer, avoid project delays by optimizing your run configurations to attain the lowest cost per sample.

When the Utmost Productivity and Efficiency are Essential
Ease of use, quality control, and speed are integral components of any next-generation sequencing workflow. Co-developed with Hitachi-Hi Technologies, the 5500xl-W Genetic Analyzer's elegant tabletop design (Figure 1) is built off “Six-Sigma" processes to streamline your research. The 5500xl-W Genetic Analyzer delivers streamlined fluidics and a simple, intuitive graphical user interface (GUI) for easy setup and run monitoring. Real-time analysis and 60% smaller data footprints expedite data export and analysis time. Additionally, with embedded sequencing controls and reagent usage tracking, the 5500xl-W Genetic Analyzer empowers you to obtain extremely high-quality and biologically-meaningful data out of every sequencing run.

Take Comprehensive Research to New Heights
The superior accuracy, flexibility, and productivity of the 5500xl-W Genetic Analyzer accelerate comprehensive research. Larger translational medicine initiatives, genome consortiums, and disease-subtype stratification projects with resulting groundbreaking publications are now within your grasp. Catch them all with the 5500xl-W Genetic Analyzer.

A Community of Support
With more than 2,000 field personnel, we have the most extensive network of dedicated Field Application Specialists and Service Engineers to work with you, so that you can feel confident that we will be there when and where you need us most.

This product includes:
Scientific CMOS camera and tube lens assembly
New high efficiency objective lens
Mounting accessories and communication cables

Recommended Kit:
The Computer Upgrade Kit is highly recommended to optimize performance of the upgraded system.

The AB Library Builder™ D-Rings are an accessory that is used with the AB Library Builder™ System. They can be ordered separately as maintenance replacements for the D-Rings that come with a system purchase or as extras to supplement your throughput. The AB Library Builder™ D-Rings come in sets of 13 replacement rings.

Foot Switch for the Elga® PURELAB® flex.

Dispense water using your foot.

For Research Use Only. Not for use in diagnostics procedures.

This is a 96-well Plate Base (Heat Seal) for use with Applied Biosystems® 3730 and 3730xl DNA Analyzers.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

The IonCode Barcode Adapters 1-384 Kit for Agrigenomics provides a set of unique barcode adapters designed for optimal performance with Ion semiconductor sequencers. When used in combination with the AgriSeq HTS Library Kit, these barcodes enable users to pool up to 384 amplicon libraries and then conduct multiplexed sequencing analysis, helping increase lab efficiency and reduce sequencing costs per sample.

Key product features:
• Robust barcodes ligate efficiently and uniformly to generate balanced representation of libraries
• Unbiased performance helps ensure identical genotypes independent of barcode used
• Robust error correction built in by design for added confidence in sample identification

Increase sample multiplexing without impacting data quality
Multiplexing with barcoded libraries leads to more cost effective runs by permitting up to 384 samples per run, significantly decreasing the cost and handling requirements of sequencing. Each barcode has been individually evaluated to help ensure no bias of sequence context representation. The barcodes were also screened for equivalent efficiency to enable balanced read depth between libraries. Regardless of the specific barcode used, AgriSeq targeted genotyping-by-sequencing (GBS) generates highly uniform libraries and concordant genotype calls (see figure below).

These customized tubes and caps are for use with the ULTRA-TURRAX® Tube Drive from IKA® for emulsion PCR for use with the SOLiD system and 5500 series Genetic Analyzers

For Research Use Only. Not for use in diagnostics procedures.

This is an Uninterruptible Power Supply (UPS) unit to safeguard the SOLiD 4 and 5500 series Genetic Analysis Systems.

For Research Use Only. Not for use in diagnostics procedures.

This is a spare part for the 310 Genetic Analyzer.

For Research Use Only. Not for use in diagnostics procedures.

The 5500xl-W Genetic Analyzer helps achieve optimal productivity with a faster workflow, two flexible FlowChips, intuitive user workflows, and project scalability. Faster turn-around times are achieved through the introduction of a non-bead based workflow and increased throughput with a combination of higher colony density and improved optics. The 5500xl-W Genetic Analyzer offers these improvements while maintaining the benefits of the 5500 and 5500xl systems: accuracy, sensitivity, and cost effectiveness. With the 5500xl-W Genetic Analyzer, you are empowered to discover rare genetic events or sub-populations of somatic mutations at an unprecedented pace.

Key Benefits:
• Sequencing right here, right now
• Cost-effective sequencing runs with individually configurable lanes
• Superior low-frequency variant detection for whole-exome sequencing or targeted resequencing
• Reproducibility, reliability, and quality for your RNA applications
• Confidence and uniformity in multiplexing with up to 96 barcodes
• Optimal analysis solutions for streamlined productivity and freedom to operate
• New non-bead based workflow for improved project turnaround and efficiency

When Coverage is Not Enough
Additional sequence coverage fails to compensate for poor accuracy in the detection of rare variants that may hold the key to a better understanding cancer progression, disease penetrance, or drug resistance. With a system accuracy of up to 99.999%, the 5500xl-W Genetic Analyzer enables you to perform 25% less sequencing to detect rare variants than a next-generation sequencing platform with 99.99% system accuracy in order to detect somatic variant present at 1% (Figure 2). The industry-leading accuracy of the 5500xl-W Genetic Analyzer enables detection of significant biological variation for applications like whole genome resequencing, targeted resequencing, and whole transcriptome analysis.

When Flexibility, Speed, and Cost are Crucial
Multidisciplinary translational research programs often require processing of multiple samples across multiple applications like whole exome, RNA-seq, and structural variation. The 5500xl-W Genetic Analyzer provides you the flexibility to configure your sequencing runs to your project and throughput needs (Figure 3). The system’s two configurable microfluidic FlowChips process 6–12 independent samples, and the intelligent barcoding kits multiplex up to hundreds of samples in a single run. Pay-Per-Lane Sequencing (PPL-Seq™) and independent run lanes tailor the system to any project scale. The new non-bead based workflow shaves 1–2 days off project workflows for faster project data delivery. Increased colony density coupled with improved optics provides more data from each lane. With the 5500xl-W Genetic Analyzer, avoid project delays by optimizing your run configurations to attain the lowest cost per sample.

When the Utmost Productivity and Efficiency are Essential
Ease of use, quality control, and speed are integral components of any next-generation sequencing workflow. Co-developed with Hitachi-Hi Technologies, the 5500xl-W Genetic Analyzer's elegant tabletop design (Figure 1) is built off “Six-Sigma" processes to streamline your research. The 5500xl-W Genetic Analyzer delivers streamlined fluidics and a simple, intuitive graphical user interface (GUI) for easy setup and run monitoring. Real-time analysis and 60% smaller data footprints expedite data export and analysis time. Additionally, with embedded sequencing controls and reagent usage tracking, the 5500xl-W Genetic Analyzer empowers you to obtain extremely high-quality and biologically-meaningful data out of every sequencing run.

Take Comprehensive Research to New Heights
The superior accuracy, flexibility, and productivity of the 5500xl-W Genetic Analyzer accelerate comprehensive research. Larger translational medicine initiatives, genome consortiums, and disease-subtype stratification projects with resulting groundbreaking publications are now within your grasp. Catch them all with the 5500xl-W Genetic Analyzer.

A Community of Support
With more than 2,000 field personnel, we have the most extensive network of dedicated Field Application Specialists and Service Engineers to work with you, so that you can feel confident that we will be there when and where you need us most.

This product includes:
5500xl-W sequencer and a workstation computer
5500xl-W Instrument Control Software
5500xl-W sequencer accessories
5500xl-W sequencer system & library training

The Ion Proton™ System is the first benchtop sequencing system capable of human-scale genome, exome, or transcriptome sequencing in a few hours—with DNA-to-variants called in a single day.

The system combines semiconductor sequencing technology with natural biochemistry to directly translate chemical information into digital data. By leveraging the exponential improvements in the semiconductor industry (known as Moore’s Law), the Ion Proton™ Sequencing System provides an unprecedented level of scalability and flexibility to support a broad range of high throughput sequencing applications, ranging from human-scale genome to exome to transcriptome sequencing. The system’s use of the simplest natural sequencing chemistry eliminates the need for expensive optics and complex sequencing chemistries, resulting in a highly affordable sequencing system to own and operate. Real-time, direct, electrical detection of sequencing, combined with the enormous amount of computing power in both the Ion Proton™ Sequencer and Ion Proton™ Torrent Server, enables generation of high quality sequencing results from DNA library to variants in a single day.

Features of the Ion Proton™ System:

• Fastest high-throughput next-generation sequencing workflow with the fastest sequencing run-times of 2–4 hours on the Ion PI™ Chip
• Highest number of complete sequencing runs per week, with a simple and automated workflow (when used with the Ion OneTouch™ 2 System)
• Simple semiconductor sequencing workflows with robust and simple hardware that can be relied upon—no cameras, no optics, and no lasers
• Scalable, high throughput sequencing capabilities enabling up to 200-base single reads and flexible library options (e.g., fragment gDNA, targeted/exome, RNA)
• Small benchtop footprint and mounting capability (with optional rack) (two Ion Proton™ systems per rack), to deliver the only benchtop genome center
• Low-cost platform and attractively priced semiconductor chips and reagents for a range of applications
• Proven Ion semiconductor sequencing requires no complex optics and employs natural nucleotides to deliver highly accurate variant detection, uniformity of coverage, and sensitivity to detect low frequency variants
• Range of fast and simple library solutions and kits with low-input requirements for a variety of applications, such as genomic sequencing, exome sequencing, sequencing sets of genes, and RNA sequencing
• Complete end-to-end solution from base calls to variants with the Proton™ Torrent Server and Torrent Suite Software v3.0
• Simple and integrated tools for tertiary data analysis with Ion Reporter™ Software for DNA variation analysis across single, paired, or trio samples

With the availability of the groundbreaking Ion Proton™ System, rapid, high throughput sequencing is finally accessible by all laboratories.

Affordable Sequencing For Nearly Every Lab
Previous sequencing technologies have relied upon optical systems as bridges between the chemical and digital forms of sequence information. These systems have added tremendous costs to sequencers, relegating them to only the largest laboratories. By eliminating the need for the optical system, the Ion Proton™ System provides high throughput sequencing that is simpler, faster, more cost effective, and more scalable than any other technology available. With a benchtop footprint, low-cost sequencing reagents, and a simple touchscreen user interface, the Ion Proton™ System brings high throughput, next generation sequencing to nearly every lab.

Ion Power: PostLight™ Sequencing Produces Bits From Bases
The sequencing technology underlying the Ion Proton™ System exploits a well-characterized biochemical process: When a nucleotide is incorporated into a strand of DNA by a polymerase, a hydrogen ion (H+) is released as a byproduct. This hydrogen ion carries a charge that the Proton™ System’s ion sensor—essentially the world's smallest solid-state pH meter—can detect. As the sequencer floods the chip with one nucleotide after another, any nucleotide added to a DNA template will be detected as a voltage change, and the Ion Proton™ System will call the base. If a nucleotide is not a match for a particular template, no voltage change will be detected and no base will be called for that template.

Massively Parallel Sequencing On Your Bench
A principal component of the Ion Proton™ System is the sequencing chip. The Ion PI™ Chip incorporates an extremely dense array of >165 million micro-machined wells married to our proprietary ion sensor. Each well contains a different DNA template, allowing massively parallel sequencing. With the Ion PI™ Chip (165 million wells), the Ion Proton™ System enables a range of high throughput genomic applications from human-scale exome sequencing to whole transcriptome sequencing.

The Right Tool To Get Results Easily, Reliably, and Quickly
Because it detects nucleotide incorporation without the use of light, the Ion Proton™ System uses the simplest sequencing chemistry possible: natural nucleotides. There is no need for expensive and error-prone modified bases, enzymatic cascades, chemiluminescence, or fluorescence. Direct detection also means the incorporation of each nucleotide is recorded in seconds. As a result, you can do an entire sequencing run typically in a few hours.

Services and Support
Service and support plans—comprehensive plans to maximize instrument performance and ensure availability of critical systems
Qualification services—document and ensure instrument is installed, operating, and performing to manufacturer’s specifications
Training—courses for experimental design, data analysis, troubleshooting, applications, and instrument operation
Bioinformatics—tailored support for NGS informatics execution, software use, data analysis, and downstream applications
DataSafe Solution—storage and back-up strategy, design, hardware, and support for all lab data