Shop All Sample Prep Instruments for Sequencing
AB Library Builder™ System, service installation (Applied Biosystems™)
The AB Library Builder™ System simplifies next-generation sequencing by providing a semi-automated solution for DNA fragment library preparation for the SOLiD™ 4 System and 5500 Series Genetic Analysis Systems. The AB Library Builder™ System is designed to increase throughput and reduce labor associated with library preparation for exome and targeted resequencing applications. The workflow can be simplified even further by using the system for upstream DNA purification. Automated—reducing hands-on time by 50%.
Service installation is included with purchase (PN 4463776).
• Scalable: Prepares up to 13 libraries per run, up to 26 libraries per day
• Integrated: A complete system optimized for library preparation for the SOLiD™ 4 System and 5500 Series Genetic Analysis Systems.
• Automated Workflow: The AB Library Builder™ System automates the most labor-intensive parts of DNA fragment library preparation.
The AB Library Builder™ System addresses low- to medium-throughput needs for DNA fragment libraries for exome and targeted resequencing applications. It can process up to 13 samples at a time, and a second run can yield up to 26 libraries in a day. In the time needed to prepare 8 libraries manually, the AB Library Builder™ System can prepare 13 libraries.
Reagents kits for DNA fragment library creation using the AB Library Builder™ are available for purchase separately. These kits require minimal set-up and come with ready to use reagent cartridges for the creation of up to 13 DNA fragment libraries. Oligos, Barcode adaptors, and Agilent AMPure XP beads are also purchased separately. The AB Library Builder™ System provides full walk-away automation of the post-shearing and pre-nick translation portion of the DNA fragment library workflow for exome and targeted resequencing.
Note: For Research Use Only. Not intended for animal or human therapeutic or diagnostic use
Service installation is included with purchase (PN 4463776).
• Scalable: Prepares up to 13 libraries per run, up to 26 libraries per day
• Integrated: A complete system optimized for library preparation for the SOLiD™ 4 System and 5500 Series Genetic Analysis Systems.
• Automated Workflow: The AB Library Builder™ System automates the most labor-intensive parts of DNA fragment library preparation.
The AB Library Builder™ System addresses low- to medium-throughput needs for DNA fragment libraries for exome and targeted resequencing applications. It can process up to 13 samples at a time, and a second run can yield up to 26 libraries in a day. In the time needed to prepare 8 libraries manually, the AB Library Builder™ System can prepare 13 libraries.
Reagents kits for DNA fragment library creation using the AB Library Builder™ are available for purchase separately. These kits require minimal set-up and come with ready to use reagent cartridges for the creation of up to 13 DNA fragment libraries. Oligos, Barcode adaptors, and Agilent AMPure XP beads are also purchased separately. The AB Library Builder™ System provides full walk-away automation of the post-shearing and pre-nick translation portion of the DNA fragment library workflow for exome and targeted resequencing.
Note: For Research Use Only. Not intended for animal or human therapeutic or diagnostic use
Ultra-Turrax Tube Drive from IKA™ (Applied Biosystems™)
The ULTRA-TURRAX® Tube Drive from IKA® is a sample preparation instrument for use in the SOLiD™ System workflow. This instrument is required for the preparation of consistently sized reactors for emulsion PCR.
Diagenode™ Bioruptor™ Pico Ultrasonicator (Ion Torrent™)
The Diagenode™ Bioruptor® Pico Ultrasonicator is an all-in-one shearing system optimized for shearing volumes as small as 5 µL up to 2 mL. This wide range enables a variety of applications such as DNA shearing for next-generation-sequencing library preparation, RNA shearing, and chromatin shearing. Shearing is a critical step in next-generation-sequencing library preparation, chromatin immunoprecipitation, and ChIP-seq.
Key Product Features:
• All-in-one solution with advanced timing control and regulated cooling system
• Versatile—shears sample sizes from 5 µL–2 mL
• Wide range of applications: next-gen sequencing (5–100 µL), chromatin shearing (10 µL–2 mL), RNA shearing
• Higher throughput—simultaneous sonication of 6–12 samples
All Bioruptor® ultrasonicator devices are engineered to deliver highly-controlled ultrasonic energy to biological or chemical samples using Adaptive Cavitation Technology (ACT). ACT is a non-contact, parallel-processing, isothermal technology that is effective for shearing both chromatin and nucleic acids (DNA and RNA). The Bioruptor® instruments are the most cited shearing devices in the epigenetic and next-generation sequencing communities with over 900 citations in peer-reviewed journals. These instruments provide processing performance and recovery unmatched by any other sample preparation methodology. ACT allows the standardization of sample prep for a range of applications, including DNA shearing for next-generation sequencing, chromatin shearing, nucleic acid or protein extraction, cell lysis, and nanotube generation.
Key Product Features:
• All-in-one solution with advanced timing control and regulated cooling system
• Versatile—shears sample sizes from 5 µL–2 mL
• Wide range of applications: next-gen sequencing (5–100 µL), chromatin shearing (10 µL–2 mL), RNA shearing
• Higher throughput—simultaneous sonication of 6–12 samples
All Bioruptor® ultrasonicator devices are engineered to deliver highly-controlled ultrasonic energy to biological or chemical samples using Adaptive Cavitation Technology (ACT). ACT is a non-contact, parallel-processing, isothermal technology that is effective for shearing both chromatin and nucleic acids (DNA and RNA). The Bioruptor® instruments are the most cited shearing devices in the epigenetic and next-generation sequencing communities with over 900 citations in peer-reviewed journals. These instruments provide processing performance and recovery unmatched by any other sample preparation methodology. ACT allows the standardization of sample prep for a range of applications, including DNA shearing for next-generation sequencing, chromatin shearing, nucleic acid or protein extraction, cell lysis, and nanotube generation.
LiquidBiopsy™ Instrument (Ion Torrent™)
The Ion Torrent™ LiquidBiopsy™ Instrument automates isolation of circulating tumor cells (CTCs) from whole-blood samples. Achieving high target cell recovery and exceptional purity, the instrument enables downstream molecular characterization of rare cells using next-generation sequencing.
The LiquidBiopsy Instrument is part of the LiquidBiopsy™ Platform for rare cell isolation in research.
The LiquidBiopsy Platform:
• Is part of a comprehensive, cost effective, sample-to-variant workflow
• Is uniquely suited to low-input DNA sequencing without the need for whole genome amplification
• Enables highly multiplexed analyses
• Achieves a limit of detection down to <1%, critical for CTC research
• Provides rapid turnaround time, completing the sample-to-variant workflow in 2 days
Walkaway automation
The LiquidBiopsy Instrument automates flow cell priming, blood sample loading, target cell isolation, and immunofluorescence labeling of captured cells on a flow chip with the same form factor as a standard pathology slide.
Comprehensive workflow
The comprehensive, two-day LiquidBiopsy Platform sequencing workflow enables the generation of genomic research reporting data on CTCs, cell-free DNA (cfDNA), and germline whole blood cells from a single blood sample in a single cycle. Captured cells are pooled on a single Ion 318™ Chip and sequenced on the Ion PGM™ Sequencer. Resulting data may be analyzed using Ion Reporter™ Software.
High capture rate
The LiquidBiopsy Instrument uses proprietary flow chip technology that eliminates the need for whole genome amplification, a process that typically introduces false positives in the data.
The flow chip makes possible a highly pure capture for CTCs that facilitates downstream NGS analysis. The high capture rate is the result of an EpCAM-based immunocapture methodology that also allows for a cocktail of antibodies to be used for a more diverse cell recovery.
Highly sensitive multiplexing
Coupled with Ion Torrent sequencing technology, the LiquidBiopsy Platform achieves up to 100% sensitivity down to <1% allele frequency at 2,000x read depth. Using the power of Ion AmpliSeq™ technology, the platform allows for multiplexing as many as four samples, and yields more data from low-input DNA commonly obtained from CTCs. The Ion PGM Sequencer is a scalable, cost-effective sequencing solution that allows analysis of incremental numbers of samples that effectively support research applications without the need to batch samples.
Services and support
Service and support plans—comprehensive plans to maximize instrument performance and ensure availability of critical systems
Training—courses for experimental design, data analysis, troubleshooting, applications, and instrument operation
Bioinformatics—tailored support for NGS informatics execution, software use, data analysis, and downstream applications
DataSafe Solution—storage and back-up strategy, design, hardware, and support for all lab data
The LiquidBiopsy Instrument is part of the LiquidBiopsy™ Platform for rare cell isolation in research.
The LiquidBiopsy Platform:
• Is part of a comprehensive, cost effective, sample-to-variant workflow
• Is uniquely suited to low-input DNA sequencing without the need for whole genome amplification
• Enables highly multiplexed analyses
• Achieves a limit of detection down to <1%, critical for CTC research
• Provides rapid turnaround time, completing the sample-to-variant workflow in 2 days
Walkaway automation
The LiquidBiopsy Instrument automates flow cell priming, blood sample loading, target cell isolation, and immunofluorescence labeling of captured cells on a flow chip with the same form factor as a standard pathology slide.
Comprehensive workflow
The comprehensive, two-day LiquidBiopsy Platform sequencing workflow enables the generation of genomic research reporting data on CTCs, cell-free DNA (cfDNA), and germline whole blood cells from a single blood sample in a single cycle. Captured cells are pooled on a single Ion 318™ Chip and sequenced on the Ion PGM™ Sequencer. Resulting data may be analyzed using Ion Reporter™ Software.
High capture rate
The LiquidBiopsy Instrument uses proprietary flow chip technology that eliminates the need for whole genome amplification, a process that typically introduces false positives in the data.
The flow chip makes possible a highly pure capture for CTCs that facilitates downstream NGS analysis. The high capture rate is the result of an EpCAM-based immunocapture methodology that also allows for a cocktail of antibodies to be used for a more diverse cell recovery.
Highly sensitive multiplexing
Coupled with Ion Torrent sequencing technology, the LiquidBiopsy Platform achieves up to 100% sensitivity down to <1% allele frequency at 2,000x read depth. Using the power of Ion AmpliSeq™ technology, the platform allows for multiplexing as many as four samples, and yields more data from low-input DNA commonly obtained from CTCs. The Ion PGM Sequencer is a scalable, cost-effective sequencing solution that allows analysis of incremental numbers of samples that effectively support research applications without the need to batch samples.
Services and support
Service and support plans—comprehensive plans to maximize instrument performance and ensure availability of critical systems
Training—courses for experimental design, data analysis, troubleshooting, applications, and instrument operation
Bioinformatics—tailored support for NGS informatics execution, software use, data analysis, and downstream applications
DataSafe Solution—storage and back-up strategy, design, hardware, and support for all lab data
SOLiD™ EZ Bead™ Amplifier (110/220 V) (Applied Biosystems™)
The Amplifier component of the SOLiD™ EZ Bead™ System clonally amplifies library template within 3.5 hours, such that there are on average >60,000 copies of a particular library DNA fragment per bead. This level of template density is important for a strong sequencing signal. The system allows for bulk preparations to generate high yields of quality templated beads.
For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostics procedures.
Ion OneTouch™ 2 Instrument (Ion Torrent™)
The Ion OneTouch 2 Instrument performs template amplification as part of the manual workflow for the Ion PGM, Ion Proton, Ion S5, and Ion S5 XL systems. It provides scalable template preparation for all Ion semiconductor chips. The instrument has a small footprint and fits easily on any bench top.
For a fully automated workflow, please see the Ion Chef Instrument.
The Ion OneTouch ES enrichment module is not included in this purchase. For both the Ion OneTouch 2 Instrument and ES module, please see the Ion OneTouch 2 System.
Efficiency and flexibility
The Ion OneTouch 2 instrument integrates multiple manual template preparation steps into a single system. This enables parallel processing of multiple samples per day through modular design. It is flexible and supports scalable template preparation for all Ion semiconductor sequencing chips with up to 400 bases on the Ion PGM, up to 200 bases on the Ion Proton System, up to 400 bases on the Ion 520 and Ion 530 chips, and up to 200 bases on the Ion 540 chip. The need for gas is eliminated by the use of a peristaltic pump.
Contributing technologies
At the heart of the Ion OneTouch 2 Instrument are three breakthrough technologies that enable automated delivery of the template Ion Sphere particles. The first is the reaction filter that creates millions of microreactors in which clonal amplification occurs. The second is the fully integrated thermal cycler and disposable path amplification plate that enables robust thermal cycling of the microreactors. The third is the integrated centrifuge, which recovers the template Ion Sphere particles. Altogether this allows the OneTouch2 instrument to deliver massively parallel clonal amplification and recovery, all in a small bench top footprint.
Designed to fit in any lab
Coupled with the Ion OneTouch ES enrichment module (not included) the Ion OneTouch 2 Instrument enables parallel template processing with sample sequencing on the Ion PGM, Ion Proton, Ion S5, and Ion S5 XL systems. This reduces the time from sample to sequence to a single day and enables processing of multiple samples per day. The combination of Ion semiconductor sequencing and the Ion OneTouch 2 System maximizes the speed of each experiment and minimizes the overall time to completion for each project. The modular design also allows for QC of the Ion Sphere particles, to maximize experimental control and the success of each experiment.
Services and support
Service and support plans—comprehensive plans to maximize instrument performance and ensure availability of critical systems
Qualification services—document and ensure instrument is installed, operating, and performing to manufacturer’s specifications
Training—courses for experimental design, data analysis, troubleshooting, applications, and instrument operation
Bioinformatics—tailored support for NGS informatics execution, software use, data analysis, and downstream applications
DataSafe Solution—storage and back-up strategy, design, hardware, and support for all lab data
For a fully automated workflow, please see the Ion Chef Instrument.
The Ion OneTouch ES enrichment module is not included in this purchase. For both the Ion OneTouch 2 Instrument and ES module, please see the Ion OneTouch 2 System.
Efficiency and flexibility
The Ion OneTouch 2 instrument integrates multiple manual template preparation steps into a single system. This enables parallel processing of multiple samples per day through modular design. It is flexible and supports scalable template preparation for all Ion semiconductor sequencing chips with up to 400 bases on the Ion PGM, up to 200 bases on the Ion Proton System, up to 400 bases on the Ion 520 and Ion 530 chips, and up to 200 bases on the Ion 540 chip. The need for gas is eliminated by the use of a peristaltic pump.
Contributing technologies
At the heart of the Ion OneTouch 2 Instrument are three breakthrough technologies that enable automated delivery of the template Ion Sphere particles. The first is the reaction filter that creates millions of microreactors in which clonal amplification occurs. The second is the fully integrated thermal cycler and disposable path amplification plate that enables robust thermal cycling of the microreactors. The third is the integrated centrifuge, which recovers the template Ion Sphere particles. Altogether this allows the OneTouch2 instrument to deliver massively parallel clonal amplification and recovery, all in a small bench top footprint.
Designed to fit in any lab
Coupled with the Ion OneTouch ES enrichment module (not included) the Ion OneTouch 2 Instrument enables parallel template processing with sample sequencing on the Ion PGM, Ion Proton, Ion S5, and Ion S5 XL systems. This reduces the time from sample to sequence to a single day and enables processing of multiple samples per day. The combination of Ion semiconductor sequencing and the Ion OneTouch 2 System maximizes the speed of each experiment and minimizes the overall time to completion for each project. The modular design also allows for QC of the Ion Sphere particles, to maximize experimental control and the success of each experiment.
Services and support
Service and support plans—comprehensive plans to maximize instrument performance and ensure availability of critical systems
Qualification services—document and ensure instrument is installed, operating, and performing to manufacturer’s specifications
Training—courses for experimental design, data analysis, troubleshooting, applications, and instrument operation
Bioinformatics—tailored support for NGS informatics execution, software use, data analysis, and downstream applications
DataSafe Solution—storage and back-up strategy, design, hardware, and support for all lab data
SOLiD™ EZ Bead™ Emulsifier (110/220 V) (Applied Biosystems™)
This is the Emulsifier component of the SOLiD™ EZ Bead™ System. The Emulsifier combines an aqueous phase with an oil phase, creating a microreactor emulsion, bringing the necessary reagents and components for amplifying the library of interest onto a solid surface. This process takes up to 30 minutes to complete, depending on the desired bead yield.
For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostics procedures.
Covaris™ M220 NGS microTUBE Starter Kit, 15–130 μL (Ion Torrent™)
The Covaris™ M220 NGS 15–130 µL microTUBE Starter Kit is for use with the Covaris™ M220 Focused-ultrasonicator™ instrument. The instrument is designed for next-generation sequencing (NGS) applications that require high-quality DNA fragmentation for library preparation, making it a superior choice for Ion PGM™ Sequencer users. It enables tight DNA fragment distributions tunable between 150 and 1,500 bp.
The starter kit includes a microTUBE holder, 25 screw-cap microTUBEs optimized for 15 µL samples, 25 screw-cap microTUBEs optimized for 50 µL samples, and 25 snap-cap microTUBEs optimized for 130 µL samples. The kit also includes a preparation station for easy loading and unloading of sample into the microTUBEs.
The starter kit includes a microTUBE holder, 25 screw-cap microTUBEs optimized for 15 µL samples, 25 screw-cap microTUBEs optimized for 50 µL samples, and 25 snap-cap microTUBEs optimized for 130 µL samples. The kit also includes a preparation station for easy loading and unloading of sample into the microTUBEs.
Ion OneTouch™ 2 System (Ion Torrent™)
The Ion OneTouch 2 System performs template amplification and enrichment as part of the manual workflow for the Ion PGM, Ion Proton, and Ion S5 and Ion S5 XL systems. It provides scalable template preparation for all Ion semiconductor chips. With its small footprint, the system fits easily on any bench top.
For a fully automated workflow, please see the Ion Chef Instrument.
Efficiency and flexibility
The Ion OneTouch 2 System integrates multiple manual template preparation steps into a single system. This enables parallel processing of multiple samples per day through modular design. It is flexible and supports scalable template preparation for all Ion semiconductor sequencing chips with up to 400 bases on the Ion 520/530 chips, up to 200 bases on the Ion 540 chip, up to 400 bases on the Ion PGM System, and up to 200 bases on the Ion Proton System. The need for gas is eliminated by the use of a peristaltic pump.
System components
The Ion OneTouch 2 System consists of two modules: the Ion OneTouch 2 Instrument and the Ion OneTouch ES (Enrichment System). The Ion OneTouch 2 Instrument may be purchased separately.
Contributing technologies
At the heart of the Ion OneTouch 2 Instrument are three breakthrough technologies that enable automated delivery of the template Ion Sphere particles. The first is the reaction filter that creates millions of microreactors in which clonal amplification occurs. The second is the fully integrated thermal cycler and disposable path amplification plate that enables robust thermal cycling of the microreactors. The third is the integrated centrifuge, which recovers the template Ion Sphere particles. Altogether this allows the instrument to deliver massively parallel clonal amplification and recovery, all in a small bench top footprint.
Designed to fit in any lab
With the included Ion OneTouch ES module, the Ion OneTouch 2 System enables parallel template processing with sample sequencing on the Ion PGM, Ion Proton, Ion S5, and Ion S5 XL systems. This reduces the time from sample to sequence to a single day and enables processing of multiple samples per day. The combination of Ion semiconductor sequencing and the Ion OneTouch 2 System maximizes the speed of each experiment and minimizes the overall time to completion for each project. The modular design also allows for QC of the Ion Sphere particles, to maximize experimental control and the success of each experiment.
Services and support
Service and support plans—comprehensive plans to maximize instrument performance and ensure availability of critical systems
Qualification services—document and ensure instrument is installed, operating, and performing to manufacturer’s specifications
Training—courses for experimental design, data analysis, troubleshooting, applications, and instrument operation
Bioinformatics—tailored support for NGS informatics execution, software use, data analysis, and downstream applications
DataSafe Solution—storage and back-up strategy, design, hardware, and support for all lab data
For a fully automated workflow, please see the Ion Chef Instrument.
Efficiency and flexibility
The Ion OneTouch 2 System integrates multiple manual template preparation steps into a single system. This enables parallel processing of multiple samples per day through modular design. It is flexible and supports scalable template preparation for all Ion semiconductor sequencing chips with up to 400 bases on the Ion 520/530 chips, up to 200 bases on the Ion 540 chip, up to 400 bases on the Ion PGM System, and up to 200 bases on the Ion Proton System. The need for gas is eliminated by the use of a peristaltic pump.
System components
The Ion OneTouch 2 System consists of two modules: the Ion OneTouch 2 Instrument and the Ion OneTouch ES (Enrichment System). The Ion OneTouch 2 Instrument may be purchased separately.
Contributing technologies
At the heart of the Ion OneTouch 2 Instrument are three breakthrough technologies that enable automated delivery of the template Ion Sphere particles. The first is the reaction filter that creates millions of microreactors in which clonal amplification occurs. The second is the fully integrated thermal cycler and disposable path amplification plate that enables robust thermal cycling of the microreactors. The third is the integrated centrifuge, which recovers the template Ion Sphere particles. Altogether this allows the instrument to deliver massively parallel clonal amplification and recovery, all in a small bench top footprint.
Designed to fit in any lab
With the included Ion OneTouch ES module, the Ion OneTouch 2 System enables parallel template processing with sample sequencing on the Ion PGM, Ion Proton, Ion S5, and Ion S5 XL systems. This reduces the time from sample to sequence to a single day and enables processing of multiple samples per day. The combination of Ion semiconductor sequencing and the Ion OneTouch 2 System maximizes the speed of each experiment and minimizes the overall time to completion for each project. The modular design also allows for QC of the Ion Sphere particles, to maximize experimental control and the success of each experiment.
Services and support
Service and support plans—comprehensive plans to maximize instrument performance and ensure availability of critical systems
Qualification services—document and ensure instrument is installed, operating, and performing to manufacturer’s specifications
Training—courses for experimental design, data analysis, troubleshooting, applications, and instrument operation
Bioinformatics—tailored support for NGS informatics execution, software use, data analysis, and downstream applications
DataSafe Solution—storage and back-up strategy, design, hardware, and support for all lab data
Ion Chef™ Instrument (Ion Torrent™)
The Ion Chef System is the next generation of workflow simplification products for the Ion S5, Ion Proton, and Ion PGM systems. The Ion Chef System provides automated library preparation, template preparation, and chip loading for users at any experience level. In less than 15 minutes of up-front hands-on time and with the use of pre-packaged library preparation reagent kits, the Ion Chef System provides a convenient walk-away workflow resulting in equalized, pooled libraries ready for templating. The automated workflow supports Ion AmpliSeq one- and two-pool designs. See www.AmpliSeq.com to design your own custom panels. Compared to the four hours of hands-on time required with the Ion OneTouch 2 System, the Ion Chef System enables significantly higher productivity. Again, in less than 15 minutes of hands-on time, the fully automated template preparation provides two chips ready for sequencing.
The Ion Chef System:
• Simplifies the workflow
• Minimizes sources of user-introduced variability
• Helps to save time and labor
• Supports sequencing preparation for the Ion S5, Ion PGM, Ion Proton systems
The Ion Chef System features:
• Automated Ion Ampliseq library preparation, template preparation, and chip loading
• Cartridge-based reagent system
• Sample and reagent-tracking capability
• Intuitive user interface
• On-board diagnostics
Simplify the Ion Torrent NGS workflow
The Ion Chef System simplifies the Ion Torrent next-generation sequencing (NGS) workflow by integrating several manual and instrument steps into a single process. The Ion Chef System workflow now incorporates: all steps of library preparation: targeted PCR amplification with respective primer panel, partial digestion of primer sequences, adapter/barcode ligation, purification, library equalization and pooling, as well as all steps of template preparation and chip loading. The simple, automated workflow provides easy access for users of any experience level.
Minimize variability and load chips reproducibly
The Ion Chef System provides reproducible chip loading by automating multiple manual chip-loading steps. The sophisticated visual system inside the Ion Chef System is designed to help reduce user-introduced error and variability by providing barcode reading and positive identification of samples, chips, and reagents. The visual system performs the set-up check before the run, as well as the reagents and consumables load and unload check, calibration, and diagnostics.
Save time and labor
The Ion Chef System enables significant time and labor savings by offering quick and easy set-up for both library preparation and templating that typically requires less than 15 minutes of hands-on time for each step.
Support for all Ion Torrent sequencing workflows
The flexible Ion Chef System is designed to provide automated and consistent Ion AmpliSeq library preparation and template preparation for sequencing on Ion S5, Ion Proton, and Ion PGM systems using any of the corresponding chips or template preparation and sequencing chemistries.
Services and support
Service and support plans—comprehensive plans to maximize instrument performance and ensure availability of critical systems
Qualification services—document and ensure instrument is installed, operating, and performing to manufacturer’s specifications
Training—courses for experimental design, data analysis, troubleshooting, applications, and instrument operation
Bioinformatics—tailored support for NGS informatics execution, software use, data analysis, and downstream applications
DataSafe Solution—storage and back-up strategy, design, hardware, and support for all lab data
The Ion Chef System:
• Simplifies the workflow
• Minimizes sources of user-introduced variability
• Helps to save time and labor
• Supports sequencing preparation for the Ion S5, Ion PGM, Ion Proton systems
The Ion Chef System features:
• Automated Ion Ampliseq library preparation, template preparation, and chip loading
• Cartridge-based reagent system
• Sample and reagent-tracking capability
• Intuitive user interface
• On-board diagnostics
Simplify the Ion Torrent NGS workflow
The Ion Chef System simplifies the Ion Torrent next-generation sequencing (NGS) workflow by integrating several manual and instrument steps into a single process. The Ion Chef System workflow now incorporates: all steps of library preparation: targeted PCR amplification with respective primer panel, partial digestion of primer sequences, adapter/barcode ligation, purification, library equalization and pooling, as well as all steps of template preparation and chip loading. The simple, automated workflow provides easy access for users of any experience level.
Minimize variability and load chips reproducibly
The Ion Chef System provides reproducible chip loading by automating multiple manual chip-loading steps. The sophisticated visual system inside the Ion Chef System is designed to help reduce user-introduced error and variability by providing barcode reading and positive identification of samples, chips, and reagents. The visual system performs the set-up check before the run, as well as the reagents and consumables load and unload check, calibration, and diagnostics.
Save time and labor
The Ion Chef System enables significant time and labor savings by offering quick and easy set-up for both library preparation and templating that typically requires less than 15 minutes of hands-on time for each step.
Support for all Ion Torrent sequencing workflows
The flexible Ion Chef System is designed to provide automated and consistent Ion AmpliSeq library preparation and template preparation for sequencing on Ion S5, Ion Proton, and Ion PGM systems using any of the corresponding chips or template preparation and sequencing chemistries.
Services and support
Service and support plans—comprehensive plans to maximize instrument performance and ensure availability of critical systems
Qualification services—document and ensure instrument is installed, operating, and performing to manufacturer’s specifications
Training—courses for experimental design, data analysis, troubleshooting, applications, and instrument operation
Bioinformatics—tailored support for NGS informatics execution, software use, data analysis, and downstream applications
DataSafe Solution—storage and back-up strategy, design, hardware, and support for all lab data
SOLiD™ EZ Bead™ Enricher (110/220 V) (Applied Biosystems™)
The Enricher component of the SOLiD™ EZ Bead™ System releases beads from the amplified reaction mixture and then selects for and retains only those beads which are templated. This process is completed within 3.5 hours.
For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostics procedures.
Ultra Turrax™ Tube Drive from IKA™ for PGM™ System (Ion Torrent™)
The ULTRA-TURRAX® Tube Drive from IKA® for the Personal Genome Machine™System is a sample preparation instrument for use in the Personal Genome Machine™ sequencing workflow. This customized benchtop device is used for the preparation of appropriately sized reactors for emulsion PCR. This device can be used in conjunction with Template Preparation Tubes.
The ULTRA-TURRAX® Tube Drive from IKA® for the PGM™ System provides you with:
• Lower cost per sample
• Scalability
For Research Use Only. Not for use in diagnostics procedures.
The ULTRA-TURRAX® Tube Drive from IKA® for the PGM™ System provides you with:
• Lower cost per sample
• Scalability
For Research Use Only. Not for use in diagnostics procedures.
SOLiD™ EZ Bead™ System (110/220 V) (Applied Biosystems™)
The SOLiD™ EZ Bead™ System simplifies next-generation sequencing workflows by providing an automated solution for reproducible templated bead preparation with less than 1 hour of hands-on time. The system is specifically designed with system modularity and quality control check points for greater experimental control. The SOLiD™ EZ Bead™ System streamlines the sequencing workflow through significant time savings and support for up to 96 multiplexed libraries. With automation, minimal costs, and seamless effort, the SOLiD™ EZ Bead™ System dramatically increases sequencing productivity to further accelerate scientific discovery.
For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostics procedures.
AB Library Builder™ System, self installation (Applied Biosystems™)
The AB Library Builder™ System simplifies next-generation sequencing by providing a semi-automated solution for DNA fragment library preparation for the SOLiD™ 4 System and 5500 Series Genetic Analysis Systems. The AB Library Builder™ System is designed to increase throughput and reduce labor associated with library preparation for exome and targeted resequencing applications. The workflow can be simplified even further by using the system for upstream DNA purification. Automated—reducing hands-on time by 50%.
Purchased for self installation (PN4463592).
• Scalable: Prepares up to 13 libraries per run, up to 26 libraries per day
• Integrated: A complete system optimized for library preparation for the SOLiD™ 4 System and 5500 Series Genetic Analysis Systems.
• Automated Workflow: The AB Library Builder™ System automates the most labor-intensive parts of DNA fragment library preparation.
The AB Library Builder™ System addresses low- to medium-throughput needs for DNA fragment libraries for exome and targeted resequencing applications. It can process up to 13 samples at a time, and with a second run can yield up to 26 libraries in a day. In the time needed to prepare 8 libraries manually, the AB Library Builder™ System can prepare 13 libraries.
Reagents kits for DNA fragment library creation using the AB Library Builder™ are available for purchase separately. These kits require minimal set-up and come with ready to use reagent cartridges for the creation of up to 13 DNA fragment libraries. Oligos, Barcode adaptors, and Agilent AMPure XP beads are also purchased separately.
The AB Library Builder™ System provides full walk-away automation of the post-shearing and pre-nick translation portion of the DNA fragment library workflow for exome and targeted resequencing.
Note: For Research Use Only. Not intended for animal or human therapeutic or diagnostic use
Purchased for self installation (PN4463592).
• Scalable: Prepares up to 13 libraries per run, up to 26 libraries per day
• Integrated: A complete system optimized for library preparation for the SOLiD™ 4 System and 5500 Series Genetic Analysis Systems.
• Automated Workflow: The AB Library Builder™ System automates the most labor-intensive parts of DNA fragment library preparation.
The AB Library Builder™ System addresses low- to medium-throughput needs for DNA fragment libraries for exome and targeted resequencing applications. It can process up to 13 samples at a time, and with a second run can yield up to 26 libraries in a day. In the time needed to prepare 8 libraries manually, the AB Library Builder™ System can prepare 13 libraries.
Reagents kits for DNA fragment library creation using the AB Library Builder™ are available for purchase separately. These kits require minimal set-up and come with ready to use reagent cartridges for the creation of up to 13 DNA fragment libraries. Oligos, Barcode adaptors, and Agilent AMPure XP beads are also purchased separately.
The AB Library Builder™ System provides full walk-away automation of the post-shearing and pre-nick translation portion of the DNA fragment library workflow for exome and targeted resequencing.
Note: For Research Use Only. Not intended for animal or human therapeutic or diagnostic use
Ion Chip™ Minifuge, 230 V (Ion Torrent™)
The personal-sized Ion Chip™ MiniFuge is designed and calibrated specifically for centrifugation of Ion PGM™/Ion Proton™ chips, enabling effective and efficient sample loading. The MiniFuge features a compact design with a small footprint. The cylindrical shape of the Ion Chip™ Minifuge allows it to accommodate both the Ion PGM™ and the larger Ion Proton™ chips. The unit quickly accelerates to maximum speed and stops quickly.
• Tested and validated by Ion Torrent
• Spins Ion PGM™ and Ion Proton™ chips, enabling effective and efficient sample loading
• Small footprint, compact design
The Ion Chip™ Minifuge is supplied with one Ion PGM™ rotor and two Ion PGM™ chip buckets. An Ion Proton™ rotor and chip buckets are not included with this unit, but are included with the Ion Proton™ System and installed in the MiniFuge by the field service engineer at the time of system installation.
The Ion Chip™ Minifuge features an on/off power switch for basic operational control. With the switch in the "on" position, the rotor starts spinning when the transparent lid is closed. The rotor will stop spinning when the lid release button is pressed. The Ion Chip™ Minifuge requires less than six square inches of bench space.
• Tested and validated by Ion Torrent
• Spins Ion PGM™ and Ion Proton™ chips, enabling effective and efficient sample loading
• Small footprint, compact design
The Ion Chip™ Minifuge is supplied with one Ion PGM™ rotor and two Ion PGM™ chip buckets. An Ion Proton™ rotor and chip buckets are not included with this unit, but are included with the Ion Proton™ System and installed in the MiniFuge by the field service engineer at the time of system installation.
The Ion Chip™ Minifuge features an on/off power switch for basic operational control. With the switch in the "on" position, the rotor starts spinning when the transparent lid is closed. The rotor will stop spinning when the lid release button is pressed. The Ion Chip™ Minifuge requires less than six square inches of bench space.
