Shop All Primary Cells
Cryopreserved Human Kupffer Cells (Gibco™)
Cryopreserved Human Kupffer Cells are isolated from adult human livers. Each vial contains a minimum of one million viable cells. These easy-to-use cells provide a convenient way to produce hepatocyte and Kupffer cell co-cultures for the study of various hepatic functions.These cells typically have high viabilities and in vivo-like expression levels.
• Viability routinely >90%
• Purity routinely >90%
• Respond to activation with lipopolysaccharides (LPS)
• Minimum 1 million viable cells per vial
• Protocols for hepatocyte co-culture available
Kupffer Cells Provide for Improved Physiological Modeling
Growing evidence shows that under both normal and pathological conditions, many hepatocyte functions are regulated by substances released from neighboring non-parenchymal cells (NPC). These cells, particularly Kupffer cells, play an important role in the modulation of xenobiotic metabolism in the liver. Kupffer cells secrete potent mediators of the inflammatory response that controls liver inflammation. These cytokine mediators control hepatocyte metabolic rates through direct interactions with phase I and phase II enzymes. Kupffer cell and hepatocyte co-cultures can self-assemble within 72 hours of treatment with pro-inflammatory cytokines or LPS, and can function by effectively modulating P450 expression, thus giving a more physiologically relevant result. Studies indicate that co-culture of hepatocytes with NPCs better represent both normal liver physiology as well as disease states.
• Viability routinely >90%
• Purity routinely >90%
• Respond to activation with lipopolysaccharides (LPS)
• Minimum 1 million viable cells per vial
• Protocols for hepatocyte co-culture available
Kupffer Cells Provide for Improved Physiological Modeling
Growing evidence shows that under both normal and pathological conditions, many hepatocyte functions are regulated by substances released from neighboring non-parenchymal cells (NPC). These cells, particularly Kupffer cells, play an important role in the modulation of xenobiotic metabolism in the liver. Kupffer cells secrete potent mediators of the inflammatory response that controls liver inflammation. These cytokine mediators control hepatocyte metabolic rates through direct interactions with phase I and phase II enzymes. Kupffer cell and hepatocyte co-cultures can self-assemble within 72 hours of treatment with pro-inflammatory cytokines or LPS, and can function by effectively modulating P450 expression, thus giving a more physiologically relevant result. Studies indicate that co-culture of hepatocytes with NPCs better represent both normal liver physiology as well as disease states.
Mouse (CD-1) Cryopreserved Hepatocytes, Plateable Male (Gibco™)
These plateable cryopreserved male mouse (CD-1) hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.
Cells per vial: 4-8 million
Get the Results You Need with GIBCO® Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure that you receive the highest quality cryopreserved hepatocytes.
Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%
• Attachment efficiency
• Monolayer formation and integrity
For research use only. Not intended for human or animal therapeutic or diagnostic use.
Related Links:
Learn more about our cryopreserved hepatocytes
Learn about our full line of hepatocytes ,
Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.
Cells per vial: 4-8 million
Get the Results You Need with GIBCO® Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure that you receive the highest quality cryopreserved hepatocytes.
Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%
• Attachment efficiency
• Monolayer formation and integrity
For research use only. Not intended for human or animal therapeutic or diagnostic use.
Related Links:
Learn more about our cryopreserved hepatocytes
Learn about our full line of hepatocytes ,
Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.
Mouse (CD-1) Cryopreserved Hepatocytes, Plateable Female (Gibco™)
These plateable cryopreserved female mouse (CD-1) hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.
Cells per vial: 4-8 million
Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.
Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%
• Attachment efficiency
• Monolayer formation and integrity
For research use only. Not intended for human or animal therapeutic or diagnostic use.
Related Links:
Learn more about our cryopreserved hepatocytes
Learn about our full line of hepatocytes ,
Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.
Cells per vial: 4-8 million
Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.
Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%
• Attachment efficiency
• Monolayer formation and integrity
For research use only. Not intended for human or animal therapeutic or diagnostic use.
Related Links:
Learn more about our cryopreserved hepatocytes
Learn about our full line of hepatocytes ,
Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.
Rat Primary Cortical Astrocytes (Gibco™)
Ready-to use system: highly pure cells, superior cell morphology and post-thaw viability, and excellent cell expansion. With GIBCO® Rat Primary Cortical Astrocytes, we offer neuroscience researchers an ideal, ready-to-use substitute to freshly isolated astrocytes. The cells are isolated from the cortex of Sprague-Dawley at day E19 of gestation and passage once before cryopreservation. Each vial contains 1 × 106 cells/ml viable cells (post-thaw). Unlike the other commercially available primary rat astrocytes that either yield about 50% post-thaw viability or form aggregates upon thawing, Gibco® Rat Primary Cortical Astrocytes consistently give 70% or more post-thaw viability. Astrocytes are the largest and most numerous neuroglial cells in the brain and the spinal cord. Astrocytes are known to regulate the extracellular ionic and chemical environment, respond to CNS injury, and play a fundamental role in the pathogenesis of ischemic neuronal death. In addition, astrocytes have been frequently used in the study of neuroglial interactions, blood brain barrier, stroke and other disease models. All these make astrocytes valuable source in cell biology studies, in animal model development and transplantation, and potentially in cell therapy applications.
Human Aortic Smooth Muscle Cells (HASMC) (Gibco™)
Primary human aortic smooth muscle cells cryopreserved at the end of the tertiary culture.
• Viability at least 70%
• ≥500,000 viable cells/vial
• Capable of at least 16 population doublings
• Mycoplasma: Not Detected
• Hepatitis B: Not Detected
• Hepatitis C: Not Detected
• HIV-1: Not Detected
• Bacteria, yeast, and other fungi: Not Detected
• Negative for von Willebrand factor (vWf)
• Positive for α-actin
• Viability at least 70%
• ≥500,000 viable cells/vial
• Capable of at least 16 population doublings
• Mycoplasma: Not Detected
• Hepatitis B: Not Detected
• Hepatitis C: Not Detected
• HIV-1: Not Detected
• Bacteria, yeast, and other fungi: Not Detected
• Negative for von Willebrand factor (vWf)
• Positive for α-actin
Human Skeletal Myoblasts (Gibco™)
Primary Normal Human Skeletal Myoblasts (HSkM) are designed to undergo highly efficient differentiation directly following plating of cryopreserved cells. Each vial contains cells that have been cryopreserved in a medium containing 10% DMSO and will differentiate within 48 hours after plating. These cells do not require expansion prior to differentiation. Cell viability is >70% post thaw with a ≥50% myogenic index after 48 hours. Intended for use by researchers investigating the molecular and biochemical bases of various normal and disease processes. They are compatible with Bacmam delivery technologies for various downstream applications. Large size contains 4.5 x 10^6 ≤ N ≤ 7.5 x 10^6 cells per vial and small size contains 1.1 x 10^6 ≤ N ≤ 1.9 x 10^6 cells per vial.
Mouse (CD-1) Cryopreserved Hepatocytes, Male (Gibco™)
These cryopreserved male mouse (CD-1) hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.
Cells per vial: 4-8 million
Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.
Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%
For research use only. Not intended for human or animal therapeutic or diagnostic use.
Related Links:
Learn more about our cryopreserved hepatocytes
Learn about our full line of hepatocytes ,
Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.
Cells per vial: 4-8 million
Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.
Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%
For research use only. Not intended for human or animal therapeutic or diagnostic use.
Related Links:
Learn more about our cryopreserved hepatocytes
Learn about our full line of hepatocytes ,
Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.
Mouse (CD-1) Cryopreserved Hepatocytes, Female (Gibco™)
These cryopreserved female mouse (CD-1) hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.
Cells per vial: 4-8 million
Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.
Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%
For research use only. Not intended for human or animal therapeutic or diagnostic use.
Related Links:
Learn more about our cryopreserved hepatocytes
Learn about our full line of hepatocytes ,
Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.
Cells per vial: 4-8 million
Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.
Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%
For research use only. Not intended for human or animal therapeutic or diagnostic use.
Related Links:
Learn more about our cryopreserved hepatocytes
Learn about our full line of hepatocytes ,
Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.
C57BL/6 Mouse Embryonic Fibroblasts, MitC-treated (Gibco™)
Mitomycin C (MitC)-treated Gibco C57BL/6 Mouse Embryonic Fibroblasts (MEFs) are feeder cells that support the culture of healthy undifferentiated embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), especially mouse ESCs/iPSCs. Manufactured by MTI-GlobalStem and equivalent to MTI-GlobalStem Cat. No. GSC-6002M, these MEF cells are isolated from inbred C57BL/6 mice, mitotically arrested by MitC treatment, and tested to help ensure performance and minimize the risk of contamination. For our most widely used feeder cells for culturing pluripotent stem cells, see CF1 Mouse Embryonic Fibroblasts, Irradiated or CF1 Mouse Embryonic Fibroblasts, MitC-Treated. You may also choose from our selection of drug-resistant MEFs to suit your application.
• Help save precious time with pre-inactivated, ready-to-use MEFs
• Culture ESCs and iPSCs with confidence using meticulously tested feeder cells
• Provide optimum support for mouse ESCs and iPSCs
Help save time with ready-to-use MEFs
MitC-treated C57BL/6 MEFs help save time and trouble dealing with an animal facility, performing tedious, time-consuming dissections, and expanding and testing cells day after day, week after week. Focus on your research with these pre-inactivated, ready-to-use feeders.
Culture with meticulously tested feeder cells
Each lot of MitC-treated C57BL/6 MEFs is tested for bacteria, fungi, mycoplasma, and mouse pathogens to minimize the risk of contamination. Each lot is also tested for post-thaw viability (typically greater than 95%) and ability to support mouse and human ESCs to help ensure robust and consistent performance. Culture your ESCs and iPSCs with confidence, knowing that you can rely on these high quality feeder cells.
Provide optimum support for mouse ESCs and iPSCs
While C57BL/6 MEFs can also support human ESCs and iPSCs, these feeder cells work particularly well for culturing mouse ESCs and iPSCs, showing better results compared to CF1 and ICR strain MEFs.
• Help save precious time with pre-inactivated, ready-to-use MEFs
• Culture ESCs and iPSCs with confidence using meticulously tested feeder cells
• Provide optimum support for mouse ESCs and iPSCs
Help save time with ready-to-use MEFs
MitC-treated C57BL/6 MEFs help save time and trouble dealing with an animal facility, performing tedious, time-consuming dissections, and expanding and testing cells day after day, week after week. Focus on your research with these pre-inactivated, ready-to-use feeders.
Culture with meticulously tested feeder cells
Each lot of MitC-treated C57BL/6 MEFs is tested for bacteria, fungi, mycoplasma, and mouse pathogens to minimize the risk of contamination. Each lot is also tested for post-thaw viability (typically greater than 95%) and ability to support mouse and human ESCs to help ensure robust and consistent performance. Culture your ESCs and iPSCs with confidence, knowing that you can rely on these high quality feeder cells.
Provide optimum support for mouse ESCs and iPSCs
While C57BL/6 MEFs can also support human ESCs and iPSCs, these feeder cells work particularly well for culturing mouse ESCs and iPSCs, showing better results compared to CF1 and ICR strain MEFs.
Primary Rat Cortex Neurons (Gibco™)
GIBCO Primary Rat Cortex Neurons are isolated from day-18 rat embryos and cryopreserved in liquid nitrogen. Each vial contains 1x10e6 cells⁄ml viable cells (post-thaw). With the cryopreserved Primary Rat Cortex Neurons (E-18), Invitrogen offers neuroscience researchers a flexible, ready-to-use, and quality alternative to acutely isolated neurons. Besides being convenience, these products are developed through vigorous internal research to afford comparable properties, such as viability and cell function, with cells that are acutely isolated. They are also highly pure cells containing minimum amount of astrocytes and other glial cells, and are with minimal lot-to-lot variability because of large single lot manufacturing. Unlike the other commercially available primary rat neurons that generally yield about 30% or lower post-thaw viability, Invitrogen cells consistently give post-thaw viability in the range of 50-80%.
Human Coronary Artery Smooth Muscle Cells (HCASMC) (Gibco™)
Primary human coronary artery smooth muscle cells cryopreserved at the end of the tertiary culture.
• Viability at least 70%
• ≥500,000 viable cells/vial
• Capable of at least 16 population doublings
• Mycoplasma: Not Detected
• Hepatitis B: Not Detected
• Hepatitis C: Not Detected
• HIV-1: Not Detected
• Bacteria, yeast, and other fungi: Not Detected
• Negative for von Willebrand factor (vWf)
• Positive for a-actin
• Viability at least 70%
• ≥500,000 viable cells/vial
• Capable of at least 16 population doublings
• Mycoplasma: Not Detected
• Hepatitis B: Not Detected
• Hepatitis C: Not Detected
• HIV-1: Not Detected
• Bacteria, yeast, and other fungi: Not Detected
• Negative for von Willebrand factor (vWf)
• Positive for a-actin
Human Plateable Hepatocytes, Transporter Qualified (Gibco™)
Plateable cryopreserved human hepatocytes assessed for transporter function using uptake transporter assays at both 4°C and 37°C.
Cells per vial: 4-8 million
Hepatocyte cultures were incubated with radiolabeled taurocholate, digoxin, and estradiol 17β glucuronide (E2-17G), substrates of the uptake and efflux transporters NTCP and BSEP, OCT1 and MDR1, and OATPs and MRP2, respectively. Accumulation rates and biliary excretion index (BEI%) were calculated.
Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.
Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%
• Attachment efficiency
• Monolayer formation and integrity
For research use only. Not intended for human or animal therapeutic or diagnostic use.
Related Links:
Learn more about our cryopreserved hepatocytes
Learn about our full line of hepatocytes ,
Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.
Cells per vial: 4-8 million
Hepatocyte cultures were incubated with radiolabeled taurocholate, digoxin, and estradiol 17β glucuronide (E2-17G), substrates of the uptake and efflux transporters NTCP and BSEP, OCT1 and MDR1, and OATPs and MRP2, respectively. Accumulation rates and biliary excretion index (BEI%) were calculated.
Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.
Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%
• Attachment efficiency
• Monolayer formation and integrity
For research use only. Not intended for human or animal therapeutic or diagnostic use.
Related Links:
Learn more about our cryopreserved hepatocytes
Learn about our full line of hepatocytes ,
Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.
Human Dermal Fibroblasts, adult (HDFa) (Gibco™)
Primary human dermal fibroblasts isolated from adult skin, cryopreserved at the end of the primary culture.
• Viability at least 70%
• ≥500,000 viable cells/vial
• Capable of at least 12 population doublings
• Mycoplasma: Not Detected
• Hepatitis B: Not Detected
• Hepatitis C: Not Detected
• HIV-1: Not Detected
• Bacteria, yeast, and other fungi: Not Detected
• Viability at least 70%
• ≥500,000 viable cells/vial
• Capable of at least 12 population doublings
• Mycoplasma: Not Detected
• Hepatitis B: Not Detected
• Hepatitis C: Not Detected
• HIV-1: Not Detected
• Bacteria, yeast, and other fungi: Not Detected
Primary Mouse Cortical Neurons (Gibco™)
Gibco® Primary Mouse Cortical Neurons are isolated and cryopreserved from C57BL/6 embryonic day-17 mice. A flexible, ready-to-use, and quality alternative to fresh neurons, each vial contains 1 million cells per mL. These neurons consistently yield post-thaw viability in the range of 50-80%, have high neuronal purity, and are validated for functionality.
• High viability: post-thaw viability of 50% or greater
• High purity: greater than 98% neuronal phenotype
• Functionally validated: tested for long-term survival, neurotransmitter response, and transduction
• High viability: post-thaw viability of 50% or greater
• High purity: greater than 98% neuronal phenotype
• Functionally validated: tested for long-term survival, neurotransmitter response, and transduction
Fish (rainbow trout) Cryopreserved Hepatocytes, Male (Gibco™)
These cryopreserved male rainbow trout (Oncorhynchus mykiss) hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.
Cells per vial: 4-8 million
Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.
Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%
For research use only. Not intended for human or animal therapeutic or diagnostic use.
Related Links:
Learn more about our cryopreserved hepatocytes
Learn about our full line of hepatocytes ,
Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.
Cells per vial: 4-8 million
Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.
Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%
For research use only. Not intended for human or animal therapeutic or diagnostic use.
Related Links:
Learn more about our cryopreserved hepatocytes
Learn about our full line of hepatocytes ,
Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.
