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SMD1168, Pichia pastoris Yeast Strain (Invitrogen™)
Several Pichia pastoris strains are available to allow you to optimize expression and recovery of your protein of interest. Table 1 provides information to help you choose the appropriate strain.
X-33, Pichia pastoris Yeast Strain (Invitrogen™)
Several Pichia pastoris strains are available to allow you to optimize expression and recovery of your protein of interest. Table 1 provides information to help you choose the appropriate strain.
Flp-In™ T-REx™ 293 Cell Line (Invitrogen™)
Flp-In™ 293 T-REx cell lines is designed for rapid generation of stable cell lines that ensure homogenous expression of your protein of interest from a Flp-In™ expression vector. These cells contain a single stably integrated FRT site at a transcriptionally active genomic locus. Targeted integration of a Flp-In™ expression vector ensures high-level expression of your gene of interest. The Flp-In™ T-REx™-293 Cell Line contains pFRT⁄lacZeo and pcDNA™6⁄TR (from the T-REx™ System) stably integrated. Co-transfection of the Flp-In™ Cell Lines with a Flp-In™ expression vector and the Flp recombinase vector, pOG44, results in targeted integration of the expression vector to the same locus in every cell, ensuring homogeneous levels of gene expression.
The Flp-In™-293 work well with Flp-In™ vectors that express a gene from the CMV promoter (e.g., pcDNA™5⁄FRT, pcDNA5⁄FRT⁄V5-His-TOPO®, and pSecTag⁄FRT⁄V5-His-TOPO®).
The Flp-In™-293 work well with Flp-In™ vectors that express a gene from the CMV promoter (e.g., pcDNA™5⁄FRT, pcDNA5⁄FRT⁄V5-His-TOPO®, and pSecTag⁄FRT⁄V5-His-TOPO®).
T-REx™-CHO Cell Line (Invitrogen™)
T-REx™ Cell Lines stably express the tetracycline repressor protein (Table 1). They save significant time and effort when using the T-REx™ System. The T-REx™ Cell Lines are functionally tested by transient transfection with the positive control vector pcDNA™4⁄TO⁄lacZ. T-REx™ Cell Lines exhibit extremely low basal expression levels in the repressed state and high expression upon induction with tetracycline (Figure 1).
GS115, Pichia pastoris Yeast Strain (Invitrogen™)
Several Pichia pastoris strains are available to allow you to optimize expression and recovery of your protein of interest. Table 1 provides information to help you choose the appropriate strain.
293FT Cell Line (Invitrogen™)
La lignée cellulaire 293FT est idéale pour générer le lentivirus à titre élevé à l’aide du système d’expression lentivirale ViraPower™. La lignée cellulaire 293FT est un isolat clonal hautement transfectable et à fort taux de croissance, qui dérive des cellules rénales embryonnaires humaines transformées avec l’antigène grand T SV40. Lorsqu’un vecteur d’expression ViraPower™ et le mélange d’emballage lentiviral ViraPower™ sont co-transfectés dans les cellules 293FT, des niveaux élevés d’ARN viral et de protéines Gag⁄Pol et Rev, requises pour l’emballage, sont produits.
Avantages
• Fort taux de croissance
• Hautement transfectable
• Production de titres viraux élevés
• Permet d’exprimer de très hauts niveaux de protéines
Caractéristiques principales :
• La présence de l’antigène grand T SV40 permet d’exprimer de très hauts niveaux de protéines à partir de vecteurs contenant l’origine de SV40. En fait, ces niveaux sont supérieurs à ceux des 293 autres cellules ou des cellules COS transformées par l’antigène T, d’utilisation courante.
Ce kit comprend :
• La lignée cellulaire 293FT fournie en tant que souche congelée dans 1 ml de milieux complets à 90 % et de diméthylsulfoxyde (DMSO) à 10 %
Avantages
• Fort taux de croissance
• Hautement transfectable
• Production de titres viraux élevés
• Permet d’exprimer de très hauts niveaux de protéines
Caractéristiques principales :
• La présence de l’antigène grand T SV40 permet d’exprimer de très hauts niveaux de protéines à partir de vecteurs contenant l’origine de SV40. En fait, ces niveaux sont supérieurs à ceux des 293 autres cellules ou des cellules COS transformées par l’antigène T, d’utilisation courante.
Ce kit comprend :
• La lignée cellulaire 293FT fournie en tant que souche congelée dans 1 ml de milieux complets à 90 % et de diméthylsulfoxyde (DMSO) à 10 %
FreeStyle™ 293-F Cells (Gibco™)
La lignée cellulaire FreeStyle™ 293-F est dérivée de la lignée cellulaire 293. Elle est utilisée avec le système d’expression FreeStyle™ MAX 293 ou FreeStyle™ 293. Les cellules FreeStyle™ 293-F sont adaptées à la culture en suspension dans le milieu d’expression FreeStyle™ 293. Les cellules congelées sont disponibles et peuvent être décongelées directement dans le milieu d’expression FreeStyle™ 293. La lignée cellulaire FreeStyle™ 293-F est fournie dans un flacon contenant 1 ml de cellules à 1 x 107 cellules viables/ml dans le milieu d’expression FreeStyle™ 293 à 90 % et dans le diméthylsulfoxyde (DMSO) à 10 %. Caractéristiques des cellules FreeStyle™ 293-F :
• Préparées dans des cultures de banque cellulaire maîtresse à faible passage, dérivées des cellules parentales 293-F qui ont été reclonées en limitant la dilution. Les cultures dérivées clonales 293 sont conservées sans sérum pour 30 à 35 passages au total uniquement.
• Adaptées à la croissance en suspension, à haute densité et sans sérum. Possibilité de conservation dans le milieu d’expression FreeStyle™ 293.
• Efficacité de transfection exceptionnelle avec le réactif FreeStyle™ MAX.
• Les cultures en suspension peuvent être transfectées dans le milieu d’expression FreeStyle™ 293 sans devoir changer de milieu.
• Transfection des cellules en gros volumes.
• Préparées dans des cultures de banque cellulaire maîtresse à faible passage, dérivées des cellules parentales 293-F qui ont été reclonées en limitant la dilution. Les cultures dérivées clonales 293 sont conservées sans sérum pour 30 à 35 passages au total uniquement.
• Adaptées à la croissance en suspension, à haute densité et sans sérum. Possibilité de conservation dans le milieu d’expression FreeStyle™ 293.
• Efficacité de transfection exceptionnelle avec le réactif FreeStyle™ MAX.
• Les cultures en suspension peuvent être transfectées dans le milieu d’expression FreeStyle™ 293 sans devoir changer de milieu.
• Transfection des cellules en gros volumes.
Flp-In™-3T3 Cell Line (Invitrogen™)
Flp-In™ Cell Lines are designed for rapid generation of stable cell lines that express a protein of interest from a Flp-In™ expression vector. These cells contain a single stably integrated FRT site at a transcriptionally active genomic locus. Targeted integration of a Flp-In™ expression vector ensures high-level expression of your gene of interest. There are six Flp-In™ Cell Lines available for the generation of isogenic stable cell lines and a Flp-In™ T-REx™ Cell Line for the generation of tetracycline-regulated stable cell lines. Flp-In™-CV-1, Flp-In™-293, Flp-In™-BHK, Flp-In™-Jurkat, and Flp-In™-3T3 Cell Lines were created by transfecting the parent cell lines with pFRT/lacZeo and selecting for stable Zeocin™- resistant clones. The Flp-In™-CHO Cell Line was created by transfecting CHO cells with pFRT/lacZeo2 and selecting for Zeocin™-resistant clones. The Flp-In™ T-REx™-293 Cell Line contains pFRT/lacZeo and pcDNA™6/TR (from the T-REx™ System) stably integrated. Co-transfection of the Flp-In™ Cell Lines with a Flp-In™ expression vector and the Flp recombinase vector, pOG44, results in targeted integration of the expression vector to the same locus in every cell, ensuring homogeneous levels of gene expression.
Choosing your Flp-In™ Vector/Cell Line Combination
The Flp-In™-CV-1, Flp-In™-293, Flp-In™-CHO, and Flp-In™-Jurkat Cell Lines (Figure 1) work well with Flp-In™ vectors that express a gene from the CMV promoter (pcDNA™5/FRT, pcDNA5/FRT/V5-His-TOPO®, and pSecTag/FRT/V5-His-TOPO®). Flp-In™-BHK and Flp-In™-3T3 cells tend to down regulate the CMV promoter. Therefore, it is recommended that the Flp-In™ vectors containing the EF-1α promoter (pEF5/FRT/V5-DEST™ and pEF5/FRT/V5-D-TOPO®) be used with these cell lines.
Choosing your Flp-In™ Vector/Cell Line Combination
The Flp-In™-CV-1, Flp-In™-293, Flp-In™-CHO, and Flp-In™-Jurkat Cell Lines (Figure 1) work well with Flp-In™ vectors that express a gene from the CMV promoter (pcDNA™5/FRT, pcDNA5/FRT/V5-His-TOPO®, and pSecTag/FRT/V5-His-TOPO®). Flp-In™-BHK and Flp-In™-3T3 cells tend to down regulate the CMV promoter. Therefore, it is recommended that the Flp-In™ vectors containing the EF-1α promoter (pEF5/FRT/V5-DEST™ and pEF5/FRT/V5-D-TOPO®) be used with these cell lines.
CHO-S Cells (cGMP banked) and Media Kit (Gibco™)
GIBCO® CHO_S Cells (cGMP Banked) and Media Kit have been developed for the growth of Chinese Hamster Ovary (CHO) cells and expression of recombinant proteins in suspension culture. Parental CHO-S cells have been produced, banked and tested to meet cGMP quality standards. The cells have been adapted to CD CHO Medium for serum free suspension growth. The CD CHO Medium is made from animal origin free and chemically defined components. It contains no proteins, hydrolysates, or components of unknown composition. The medium is formulated without L-glutamine for greater stability, and without phenol red to minimize potential for estrogen-like effects.
Expi293F™ Cells (Gibco™)
Les cellules humaines de l’Expi293F™ sont dérivées de la lignée cellulaire 293 et forment un composant fondamental du système d’expression Expi293™. Elles sont conservées dans une culture de suspension et se développeront dans le milieu d’expression Expi293™, jusqu’à atteindre une forte densité. Les cellules Expi293F™ ont un fort pouvoir de transfection et un rendement protéinique supérieur à celui des lignées cellulaires 293 standard dans le cadre de l’expression protéinique transitoire.
• Cellules de culture en suspension pré-adaptées au milieu d’expression Expi293™m
• Niveaux d’expression protéinique supérieurs démontrés par rapport aux autres cellules 293 adaptées au milieu d’expression Expi293™
• Croissance cellulaire plus rapide et cultures plus viables que celles des cellules 293 adaptées à une suspension standard.
• Performances homogènes en matière d’expression transitoire des cellules Expi293F™ sur plus de 40 passages au-delà de la décongélation.
Les cellules congelées sont fournies dans un flacon contenant 1 ml de cellules à une concentration de 1 x 107 cellules viables/ml au sein du milieu d’expression Expi293™ et DMSO de 10 %. Les cellules doivent être décongelées directement dans le milieu d’expression Expi293™. Le pack de six flacons permet de ne pas avoir à développer ni à redéposer les cellules en laboratoire.
La croissance cellulaire s’effectue généralement dans des flacons sur une plateforme agitatrice, dans un incubateur de culture de cellules de CO2 humidifié. Les cultures sont néanmoins évolutives et peuvent être développées à partir de volumes de <1 mL in multiwell plates to >10 l au sein de bioréacteurs. Après transfection, la récolte des protéines peut s’effectuer au bout de 2 à 7 jours selon la protéine exprimée. Pour l’expression d’anticorps recombinants, la récolte s’effectue généralement entre 5 et 7 jours après la transfection. Aucune supplémentation du milieu de culture ni aucune alimentation en lot ne sont nécessaires durant l’expression.
• Cellules de culture en suspension pré-adaptées au milieu d’expression Expi293™m
• Niveaux d’expression protéinique supérieurs démontrés par rapport aux autres cellules 293 adaptées au milieu d’expression Expi293™
• Croissance cellulaire plus rapide et cultures plus viables que celles des cellules 293 adaptées à une suspension standard.
• Performances homogènes en matière d’expression transitoire des cellules Expi293F™ sur plus de 40 passages au-delà de la décongélation.
Les cellules congelées sont fournies dans un flacon contenant 1 ml de cellules à une concentration de 1 x 107 cellules viables/ml au sein du milieu d’expression Expi293™ et DMSO de 10 %. Les cellules doivent être décongelées directement dans le milieu d’expression Expi293™. Le pack de six flacons permet de ne pas avoir à développer ni à redéposer les cellules en laboratoire.
La croissance cellulaire s’effectue généralement dans des flacons sur une plateforme agitatrice, dans un incubateur de culture de cellules de CO2 humidifié. Les cultures sont néanmoins évolutives et peuvent être développées à partir de volumes de <1 mL in multiwell plates to >10 l au sein de bioréacteurs. Après transfection, la récolte des protéines peut s’effectuer au bout de 2 à 7 jours selon la protéine exprimée. Pour l’expression d’anticorps recombinants, la récolte s’effectue généralement entre 5 et 7 jours après la transfection. Aucune supplémentation du milieu de culture ni aucune alimentation en lot ne sont nécessaires durant l’expression.
T-REx™ Jurkat Cell Line (Invitrogen™)
T-REx™ Cell Lines stably express the tetracycline repressor protein (Table 1). They save significant time and effort when using the T-REx™ System. The T-REx™ Cell Lines are functionally tested by transient transfection with the positive control vector pcDNA™4⁄TO⁄lacZ. T-REx™ Cell Lines exhibit extremely low basal expression levels in the repressed state and high expression upon induction with tetracycline (Figure 1).
High Five™ Cells in Express Five™ Medium (Gibco™)
High Five™ Cells (BTI-TN-5B1-4) are a clonal isolate derived from the parental Trichoplusia ni cell line (cabbage looper ovary) and are commonly used for the expression of recombinant proteins using the Baculovirus Expression Vector System (BEVS). High Five™ Insect Cells are adapted to serum-free culture in Express Five® SFM for maximum cell growth and recombinant gene expression. High Five™ Insect Cells (frozen in Express Five® SFM) features:
• Recombinant protein expression from a variety of expression systems
• Up to ten-fold increase in secreted protein expression compared to Sf9 cells
• Good growth in adherent or suspension culture
• Quality and performance testing
Recombinant protein expression from a variety of expression systems
High levels of protein expression in High Five™ cells can be obtained using either the BaculoDirect™ Baculovirus Expression System, the Bac-to-Bac® Baculovirus Expression System, or the InsectDirect® Expression System.
Up to ten-fold increase in secreted protein expression compared to Sf9 cells
High Five™ cells are the best choice for expression of secreted protein in insect cells. They consistently express 5–10 times more protein that Sf9 cells.
Good growth in adherent or suspension culture
High Five ™ cells thaw into adherent culture, forming an irregular monolayer with plaques that may be difficult to isolate. Protocols for adherent growth can be found in Express Five® SFM are available in the product manual. High Five™ cells can be adapted to suspension culture.
Quality and performance testing
Each lot of High Five ™ cells is tested for cell growth and viability post-recovery from cryopreservation.
Caution: Handle as potentially biohazardous material under at least Biosafety Level 2 containment. This product contains Dimethyl Sulfoxide (DMSO), a hazardous material. Review the Material Safety Data Sheet before handling.
• Recombinant protein expression from a variety of expression systems
• Up to ten-fold increase in secreted protein expression compared to Sf9 cells
• Good growth in adherent or suspension culture
• Quality and performance testing
Recombinant protein expression from a variety of expression systems
High levels of protein expression in High Five™ cells can be obtained using either the BaculoDirect™ Baculovirus Expression System, the Bac-to-Bac® Baculovirus Expression System, or the InsectDirect® Expression System.
Up to ten-fold increase in secreted protein expression compared to Sf9 cells
High Five™ cells are the best choice for expression of secreted protein in insect cells. They consistently express 5–10 times more protein that Sf9 cells.
Good growth in adherent or suspension culture
High Five ™ cells thaw into adherent culture, forming an irregular monolayer with plaques that may be difficult to isolate. Protocols for adherent growth can be found in Express Five® SFM are available in the product manual. High Five™ cells can be adapted to suspension culture.
Quality and performance testing
Each lot of High Five ™ cells is tested for cell growth and viability post-recovery from cryopreservation.
Caution: Handle as potentially biohazardous material under at least Biosafety Level 2 containment. This product contains Dimethyl Sulfoxide (DMSO), a hazardous material. Review the Material Safety Data Sheet before handling.
CHO DG44 Cells (cGMP banked) and Media Kit (Gibco™)
The CHO DG44 Cells (cGMP banked) and Media Kit is optimized for the growth of dihydrofolate reductase deficient (DHFR-) Chinese Hamster Ovary (CHO) cells and expression of recombinant proteins in suspension culture. The kit provides:
GIBCO® CD DG44 Medium—a chemically defined, protein-free medium specifically designed to enable optimal growth and expression of DG44 cells in suspension culture
CHO DG44 cells (cGMP banked) —Parental CHO DG44 cells have been produced, banked and tested to meet cGMP quality standards. They are pre-adapted to CD DG44 Medium and selected for superior cell growth and transfection efficiencies.
In addition, the CHO DG44 Cells (cGMP banked) and Media Kit includes L-glutamine and Pluronic® F-68 for optimal growth and protein expression of DG44 cells in a protein-free, chemically defined environment.
GIBCO® CD DG44 Medium—a chemically defined, protein-free medium specifically designed to enable optimal growth and expression of DG44 cells in suspension culture
CHO DG44 cells (cGMP banked) —Parental CHO DG44 cells have been produced, banked and tested to meet cGMP quality standards. They are pre-adapted to CD DG44 Medium and selected for superior cell growth and transfection efficiencies.
In addition, the CHO DG44 Cells (cGMP banked) and Media Kit includes L-glutamine and Pluronic® F-68 for optimal growth and protein expression of DG44 cells in a protein-free, chemically defined environment.
Sf9 cells in Sf-900™ II SFM (Gibco™)
Gibco® Sf9 cells are commonly used to isolate and propagate recombinant baculoviral stocks and to produce recombinant proteins. Gibco® Sf9 cells are adapted to serum-free suspension culture in Gibco® Sf-900™ II SFM, which saves significant time and expense associated with the adaptation of cultures. Gibco® Sf9 cells (frozen in Gibco® Sf-900™ II SFM) feature:
• Recombinant protein expression from a variety of expression systems
• Good growth in adherent or suspension culture
• Small, regular size that generates even monolayers and plaques
• Documented lineage from a low passage Master Cell Bank
• Quality and performance testing
Recombinant protein expression from a variety of expression systems
High levels of protein expression in Sf9 cells can be obtained using either the BaculoDirect™ Baculovirus Expression System, the Bac-to-Bac® Baculovirus Expression System, or the InsectDirect® Expression System.
Small, regular size that generates even monolayers and plaques
Gibco® Sf9 cells generate a clean, even monolayer and plaques due to their small, round, regular size. Other cells often form more irregular monolayers and plaques.
Documented lineage from a low passage Master Cell Bank
Gibco® Sf9 cells (in Sf-900™ II SFM) were prepared as serum-free, suspension cultures from Sf9 cells that originated at the USDA Insect Pathology Laboratory. The original Sf9 cells were cloned from the parental IPLBSF-21 (Sf21) cell line that was derived from the pupal ovarian tissue of the fall army worm, Spodoptera frugiperda. The serum-free Master Cell Banks were prepared at passage 34.
Quality and performance testing
Each lot of Gibco® Sf9 cells is tested for cell growth and viability post-recovery from cryopreservation. In addition, the Master Seed Bank has been tested for contamination of bacteria, yeast, mycoplasma and virus and has been characterized by isozyme and karyotype analysis.
Product Use
For Research Use Only. Not for any animal or human therapeutic or diagnostic use. Caution: Handle as potentially biohazardous material under at least Biosafety Level 2 containment. This product contains Dimethyl Sulfoxide (DMSO), a hazardous material. Review the Material Safety Data Sheet before handling.
• Recombinant protein expression from a variety of expression systems
• Good growth in adherent or suspension culture
• Small, regular size that generates even monolayers and plaques
• Documented lineage from a low passage Master Cell Bank
• Quality and performance testing
Recombinant protein expression from a variety of expression systems
High levels of protein expression in Sf9 cells can be obtained using either the BaculoDirect™ Baculovirus Expression System, the Bac-to-Bac® Baculovirus Expression System, or the InsectDirect® Expression System.
Small, regular size that generates even monolayers and plaques
Gibco® Sf9 cells generate a clean, even monolayer and plaques due to their small, round, regular size. Other cells often form more irregular monolayers and plaques.
Documented lineage from a low passage Master Cell Bank
Gibco® Sf9 cells (in Sf-900™ II SFM) were prepared as serum-free, suspension cultures from Sf9 cells that originated at the USDA Insect Pathology Laboratory. The original Sf9 cells were cloned from the parental IPLBSF-21 (Sf21) cell line that was derived from the pupal ovarian tissue of the fall army worm, Spodoptera frugiperda. The serum-free Master Cell Banks were prepared at passage 34.
Quality and performance testing
Each lot of Gibco® Sf9 cells is tested for cell growth and viability post-recovery from cryopreservation. In addition, the Master Seed Bank has been tested for contamination of bacteria, yeast, mycoplasma and virus and has been characterized by isozyme and karyotype analysis.
Product Use
For Research Use Only. Not for any animal or human therapeutic or diagnostic use. Caution: Handle as potentially biohazardous material under at least Biosafety Level 2 containment. This product contains Dimethyl Sulfoxide (DMSO), a hazardous material. Review the Material Safety Data Sheet before handling.
Human MATE2K SLC Transporter Cells (Gibco™)
TRANSiPORT Human MATE2K SLC Transporter Cells are HEK293 cells that transiently overexpress the MATE2K solute carrier (SLC) transport protein. Solute carriers are a super-family of membrane transporters that can affect pharmacokinetics and drug exposure by governing the transport of solutes in and out of cells. The MATE2K SLC transporter protein is found in kidney proximal tubule cells.
• Assess potential for transporter-mediated drug metabolism
• Easy to use format
• Obtain high quality results with a large signal-to-noise ratio
Choice of measurement system
Cell-based assays can be performed using radioisotope-labeled compounds, fluorescence-labeled compounds, or non-labeled compounds. The amount of substrate transported into the cells can be measured directly using a liquid scintillation counter, fluorescence plate reader, or LC-MS/MS, thereby allowing direct evaluation of SLC transporter activity.
Assay reliability
Mock HEK293 cells (Cat. No. GM1001) that do not overexpress the SLC transporter are available for use as a negative experimental control. Some compounds may demonstrate high background levels of transport due to the presence of endogenous transporters or non-specific binding.
Rapid results
The convenient product format enables data generation in 2 days from thawing of the cells to final results.
Related products
GM1002 Human OATP1B1 Transporter Cells
GM1003 Human OAT1 Transporter Cells
GM1004 Human OAT3 Transporter Cells
GM1005 Human OCT2 Transporter Cells
GM1006 Human OATP1B3 Transporter Cells
GM1008 Human OCT1 Transporter Cells
GM1013 Human NTCP Transporter Cells
GM1014 Human MATE1 Transporter Cells
GM1001 Mock Cells
• Assess potential for transporter-mediated drug metabolism
• Easy to use format
• Obtain high quality results with a large signal-to-noise ratio
Choice of measurement system
Cell-based assays can be performed using radioisotope-labeled compounds, fluorescence-labeled compounds, or non-labeled compounds. The amount of substrate transported into the cells can be measured directly using a liquid scintillation counter, fluorescence plate reader, or LC-MS/MS, thereby allowing direct evaluation of SLC transporter activity.
Assay reliability
Mock HEK293 cells (Cat. No. GM1001) that do not overexpress the SLC transporter are available for use as a negative experimental control. Some compounds may demonstrate high background levels of transport due to the presence of endogenous transporters or non-specific binding.
Rapid results
The convenient product format enables data generation in 2 days from thawing of the cells to final results.
Related products
GM1002 Human OATP1B1 Transporter Cells
GM1003 Human OAT1 Transporter Cells
GM1004 Human OAT3 Transporter Cells
GM1005 Human OCT2 Transporter Cells
GM1006 Human OATP1B3 Transporter Cells
GM1008 Human OCT1 Transporter Cells
GM1013 Human NTCP Transporter Cells
GM1014 Human MATE1 Transporter Cells
GM1001 Mock Cells
