Shop All Magnetic Beads for Cell Isolation & Expansion
MagniSort™ Human CD117 (c-Kit) Positive Selection Kit (Invitrogen™)
The MagniSort™ Human CD117 (c-Kit) Positive Selection Kit is designed for the magnetic separation of CD117+ cells by positive selection. It has been optimized for the isolation of CD117+ cells from normal human peripheral blood cells. CD117+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from CD117+ cells by decanting.
After positive selection, the purity of selected cells can be verified by staining with Anti-Human CD117, clone YB5.B8, and Anti-Human CD34, clone 4H11.
Reported Application
Magnetic Cell Separation
After positive selection, the purity of selected cells can be verified by staining with Anti-Human CD117, clone YB5.B8, and Anti-Human CD34, clone 4H11.
Reported Application
Magnetic Cell Separation
Dynabeads™ Untouched™ Mouse CD4 Cells Kit (Invitrogen™)
Dynabeads® Untouched™ Mouse CD4 Cells contains both magnetic beads and an Antibody Mix to deplete all leucocytes from mouse spleen or lymph node cells, leaving only the target mouse CD4+ cells. The negatively isolated, untouched mouse CD4+ cells are pure, viable and ready to be used in any downstream application.
• Rapid isolation of untouched mouse CD4+ T-cells – no columns required
• Pure and viable mouse CD4+ T-cells ready for any downstream application
Excellent Yield, Purity, and Viability of Recovered Cells
The Mouse Depletion Dynabeads® suspension contains uniform, superparamagnetic beads (4.5 µm diameter) that enable easy isolation of cells from any single cell suspension. This reagent is therefore especially well-suited for enriching tissue digests such as mouse spleen and lymph node cells. The Mouse Depletion Dynabeads® are coated with a secondary polyclonal antibody that binds rat IgGs. The Antibody Mix contains a cocktail of rat IgGs that binds mouse CD8+ T cells, B cells, NK cells, monocytes/macrophages, dendritic cells, erythrocytes and granulocytes. The Antibody Mix is first added to the sample in a tube to bind the unwanted non-CD4+ cells. The cells are then washed to remove excess antibodies, and Mouse Depletion Dynabeads® are added. After a short incubation, the bead-bound cells are then separated from unbound cells in 1 – 2 minutes using a magnet. The purified target mouse CD4+ cells in the supernatant can be transferred to a new tube for use in any downstream application. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity, recovery and viability of the untouched CD4+ cells. The protocol can easily be scaled up according to your sample size.
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
• Rapid isolation of untouched mouse CD4+ T-cells – no columns required
• Pure and viable mouse CD4+ T-cells ready for any downstream application
Excellent Yield, Purity, and Viability of Recovered Cells
The Mouse Depletion Dynabeads® suspension contains uniform, superparamagnetic beads (4.5 µm diameter) that enable easy isolation of cells from any single cell suspension. This reagent is therefore especially well-suited for enriching tissue digests such as mouse spleen and lymph node cells. The Mouse Depletion Dynabeads® are coated with a secondary polyclonal antibody that binds rat IgGs. The Antibody Mix contains a cocktail of rat IgGs that binds mouse CD8+ T cells, B cells, NK cells, monocytes/macrophages, dendritic cells, erythrocytes and granulocytes. The Antibody Mix is first added to the sample in a tube to bind the unwanted non-CD4+ cells. The cells are then washed to remove excess antibodies, and Mouse Depletion Dynabeads® are added. After a short incubation, the bead-bound cells are then separated from unbound cells in 1 – 2 minutes using a magnet. The purified target mouse CD4+ cells in the supernatant can be transferred to a new tube for use in any downstream application. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity, recovery and viability of the untouched CD4+ cells. The protocol can easily be scaled up according to your sample size.
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
MagniSort™ Mouse CD8 T cell Enrichment Kit (Invitrogen™)
Le kit MagniSort™ Mouse CD8 T cell Enrichment Kit est utilisé pour la séparation magnétique des cellules T CD8 de souris par sélection négative. Il a été optimisé pour isoler des cellules T CD8 murines à partir de rates ou de ganglions lymphatiques de souris avec un mélange d’anticorps biotinylés et des microbilles magnétiques revêtues de streptavidine. Les cellules non souhaitées sont fixées aux anticorps, avant d’être immobilisées sur les microbilles magnétiques lorsqu’un champ magnétique est appliqué, laissant les cellules T CD8 intactes et libres dans la solution.
MagniSort™ Mouse CD45 Depletion Kit (Invitrogen™)
Le kit MagniSort™ Mouse CD45 Depletion Kit est utilisé pour la déplétion magnétique de cellules CD45+ murines à partir de rates, de moelle osseuse ou de tissus pulmonaires digérés de souris. Il utilise un anticorps anti-CD45 murin biotinylé et des microbilles magnétiques revêtues de streptavidine. Les cellules CD45+ sont fixées à l’anticorps, avant d’être immobilisées sur les microbilles magnétiques. Lorsqu’un champ magnétique est appliqué, les cellules CD45- sont ensuite séparées des cellules CD45+ par décantation.
Une fois la déplétion terminée, la pureté des cellules CD45- enrichies peut être vérifiée par une coloration avec l’anticorps anti-CD45, clone 30-F11 murin.
Une fois la déplétion terminée, la pureté des cellules CD45- enrichies peut être vérifiée par une coloration avec l’anticorps anti-CD45, clone 30-F11 murin.
Dynabeads™ Untouched™ Mouse CD8 Cells Kit (Invitrogen™)
Dynabeads® Untouched™ Mouse CD8 Cells supplies both 4.5 µm superparamagnetic Dynabeads® (with a polyclonal sheep anti-rat IgG antibody covalently bound to the bead surface) and a monoclonal Antibody Mix, together designed for the depletion of CD8-negative leucocytes from mouse spleen or lymph node single-cell suspensions. Following the depletion procedure, the negatively isolated, untouched target mouse CD8+ cells are pure, viable, and ready to be used in any downstream application.
• Rapid isolation of untouched mouse CD8+ T cells—no columns required
• Pure and viable mouse CD8+ T cells are ready for any downstream application
Excellent yield, purity, and viability of recovered cells
The Antibody Mix contains a cocktail of rat IgGs that binds mouse CD4+ T cells, B cells, NK cells, monocytes/macrophages, dendritic cells, erythrocytes, and granulocytes. The Antibody Mix is first added to the sample in a tube to bind the unwanted CD8-negative cells. The cells are then washed to remove excess antibodies. Mouse Depletion Dynabeads® (coated with a rat IgG antibody) are added to bind the antibody-labeled, CD8-negative cells. After a short incubation, the bead-bound cells are then separated from unbound cells in 1–2 minutes using a magnet. The purified target mouse CD8+ cells in the supernatant can be transferred to a new tube for use in any downstream application.
This fast and gentle isolation method does not require the use of columns, and helps ensure high purity, recovery and viability of the untouched CD8+ cells. The protocol can be scaled up according to your sample size.
Learn more about Dynabeads® products
• Find Dynabeads® products for a whole range of applications.
• Find magnets for Dynabeads® separations.
• Rapid isolation of untouched mouse CD8+ T cells—no columns required
• Pure and viable mouse CD8+ T cells are ready for any downstream application
Excellent yield, purity, and viability of recovered cells
The Antibody Mix contains a cocktail of rat IgGs that binds mouse CD4+ T cells, B cells, NK cells, monocytes/macrophages, dendritic cells, erythrocytes, and granulocytes. The Antibody Mix is first added to the sample in a tube to bind the unwanted CD8-negative cells. The cells are then washed to remove excess antibodies. Mouse Depletion Dynabeads® (coated with a rat IgG antibody) are added to bind the antibody-labeled, CD8-negative cells. After a short incubation, the bead-bound cells are then separated from unbound cells in 1–2 minutes using a magnet. The purified target mouse CD8+ cells in the supernatant can be transferred to a new tube for use in any downstream application.
This fast and gentle isolation method does not require the use of columns, and helps ensure high purity, recovery and viability of the untouched CD8+ cells. The protocol can be scaled up according to your sample size.
Learn more about Dynabeads® products
• Find Dynabeads® products for a whole range of applications.
• Find magnets for Dynabeads® separations.
MagniSort™ Mouse CD4 Naïve T cell Enrichment Kit (Invitrogen™)
Le kit MagniSort™ Mouse CD4 Naive T cell Enrichment Kit est utilisé pour la séparation magnétique des cellules T CD4 naïves par sélection négative. Il a été optimisé pour isoler des cellules T CD4 naïves murines à partir de rates ou de ganglions lymphatiques de souris avec un mélange d’anticorps biotinylés et des microbilles magnétiques revêtues de streptavidine. Les cellules non souhaitées sont fixées aux anticorps, avant d’être immobilisées sur les microbilles magnétiques lorsqu’un champ magnétique est appliqué, laissant les cellules T CD4 naïves intactes et libres dans la solution.
Dynabeads™ FlowComp™ Human CD8 Kit (Invitrogen™)
This gentle and easy-to-use kit allows you to isolate flow-compatible human CD8+ T-cells from PBMC or directly from whole blood. Immediately after positive isolation, the beads are released and removed from your cell sample. In this way, you avoid exposing your cells to potentially cytotoxic or immunogenic foreign substances like iron oxides or dextrans, a potential problem when working with biodegradable particles. In addition, your cells will not be exposed to the stress of being passed through a column. You can expect a purity level of >95% human CD8+ cells.
Advantages:
• No risk of your results being affected by the isolation method
• Gentle tube-based method - no columns needed
• High purity and recovery
• Bead-free cells - compatible with flow cytometry analysis
• Compatible with flow cytometry analysis
• Viable, bead-free CD8+ T-cells for direct use in any cell-based assay.
The isolated cells have the presumed profile of memory and naive CD8+ T cells, and show a high proliferation capacity. You will save time and money, and get increased confidence in your results.
Starting sample:
MNC or whole blood.
Capacity:
The kit will process 2 × 109 mononuclear cells (MNC) or 80 ml whole blood
Advantages:
• No risk of your results being affected by the isolation method
• Gentle tube-based method - no columns needed
• High purity and recovery
• Bead-free cells - compatible with flow cytometry analysis
• Compatible with flow cytometry analysis
• Viable, bead-free CD8+ T-cells for direct use in any cell-based assay.
The isolated cells have the presumed profile of memory and naive CD8+ T cells, and show a high proliferation capacity. You will save time and money, and get increased confidence in your results.
Starting sample:
MNC or whole blood.
Capacity:
The kit will process 2 × 109 mononuclear cells (MNC) or 80 ml whole blood
CELLection™ Pan Mouse IgG Kit (Invitrogen™)
Use this kit with any mouse IgG for the positive isolation of a cell population from all species except mouse. Antibodies are attached to the surface of the CELLection™ Dynabeads® via a DNA linker; this linker provides a cleavable site to release and remove the beads from the cells after isolation. Bead-free cells are viable and can be used in any downstream application.
This Kit Contains: CELLection™ Dynabeads® coated with a human anti-mouse IgG monoclonal, and DNase Releasing Buffer
Applications:
• Positively isolate and detach any cell from any species (except mouse) using your own mouse IgG
• Compatible with flow cytometry, cell culture and all cell based assays
This Kit Contains: CELLection™ Dynabeads® coated with a human anti-mouse IgG monoclonal, and DNase Releasing Buffer
Applications:
• Positively isolate and detach any cell from any species (except mouse) using your own mouse IgG
• Compatible with flow cytometry, cell culture and all cell based assays
MagniSort™ Human B Cell Enrichment Kit (2nd Generation) (Invitrogen™)
The MagniSort™ Human B cell Enrichment Kit (2nd Generation) is designed for the magnetic separation of B cells by negative selection with improved purity. It has been optimized for the isolation of human B cells from normal human peripheral blood mononuclear cells utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave B cells untouched and free in solution.
The MagniSort™ Human B cell Enrichment Antibody Cocktail (2nd Generation) contains the following antibodies:
Anti-Human CD2 Biotin
Anti-Human CD3 Biotin
Anti-Human CD11b Biotin
Anti-Human CD14 Biotin
Anti-Human CD16 Biotin
Anti-Human CD36 Biotin
Anti-Human CD56 Biotin
Anti-Human CD235a Biotin
Reported Application
Magnetic Cell Separation
The MagniSort™ Human B cell Enrichment Antibody Cocktail (2nd Generation) contains the following antibodies:
Anti-Human CD2 Biotin
Anti-Human CD3 Biotin
Anti-Human CD11b Biotin
Anti-Human CD14 Biotin
Anti-Human CD16 Biotin
Anti-Human CD36 Biotin
Anti-Human CD56 Biotin
Anti-Human CD235a Biotin
Reported Application
Magnetic Cell Separation
Dynabeads™ Sheep Anti-Rat IgG (Invitrogen™)
Dynabeads® Sheep Anti-Rat IgG are 4.5 µm superparamagnetic beads with affinity purified polyclonal sheep anti-rat IgG covalently bound to the bead surface. These beads can be used to isolate any cell from any species (except rat), depending on the specificity of the monoclonal rat IgG employed. Dynabeads® Sheep Anti-Rat IgG are especially well suited for cell isolation from mouse samples (cross-reactivity to mouse antibodies is high and cross-reactivity to human antibodies is minimal).
• Works with any cell type, from any sample and from any species (except rat)
• Fast and efficient depletion or positive isolation of any target cell for molecular downstream assays using rat primary antibody
• Obtain pure and untouched cells using a mixture of rat IgGs to deplete unwanted cells
Magnetic bead-based separation offers easy handling
The rat primary antibody may be added to the cell sample (indirect technique) or pre-coated onto the Dynabeads® Sheep Anti-Rat IgG (direct technique). Regardless of which of these techniques is used, when the cell sample and the Dynabeads® Sheep Anti-Rat IgG are mixed, the beads bind to the target cells. Placing the sample on a magnet separates the bead-bound cells from the rest of the sample. One or more rat primary antibodies may be used to either deplete the sample of unwanted cells (negative isolation) or to capture specific cells for molecular downstream experiments (positive isolation). For positive isolation of bead-free cells (e.g., for flow cytometry), we recommend using your rat antibody in combination with the Dynabeads® FlowComp™ Flexi Kit.
Useful for many sample types
Using Dynabeads® Sheep Anti-Rat IgG, you can isolate cells from many starting samples including whole blood, mouse spleen or lymph node cells, and tissue digests.
Learn more about Dynabeads® products
• Find Dynabeads® products for a whole range of applications.
• Find magnets for Dynabeads® separations.
• Works with any cell type, from any sample and from any species (except rat)
• Fast and efficient depletion or positive isolation of any target cell for molecular downstream assays using rat primary antibody
• Obtain pure and untouched cells using a mixture of rat IgGs to deplete unwanted cells
Magnetic bead-based separation offers easy handling
The rat primary antibody may be added to the cell sample (indirect technique) or pre-coated onto the Dynabeads® Sheep Anti-Rat IgG (direct technique). Regardless of which of these techniques is used, when the cell sample and the Dynabeads® Sheep Anti-Rat IgG are mixed, the beads bind to the target cells. Placing the sample on a magnet separates the bead-bound cells from the rest of the sample. One or more rat primary antibodies may be used to either deplete the sample of unwanted cells (negative isolation) or to capture specific cells for molecular downstream experiments (positive isolation). For positive isolation of bead-free cells (e.g., for flow cytometry), we recommend using your rat antibody in combination with the Dynabeads® FlowComp™ Flexi Kit.
Useful for many sample types
Using Dynabeads® Sheep Anti-Rat IgG, you can isolate cells from many starting samples including whole blood, mouse spleen or lymph node cells, and tissue digests.
Learn more about Dynabeads® products
• Find Dynabeads® products for a whole range of applications.
• Find magnets for Dynabeads® separations.
Dynabeads™ CD25 (Invitrogen™)
Isolate CD25+ T-cells directly from whole blood or PBMC. Dynabeads® CD25 are ideal for depleting CD25+ T-cells and for direct positive isolation for downstream molecular analysis.
• Depletion of CD25+ T-cells
• Isolation of pure and viable CD25+ T-cells for molecular applications
• No columns required
Note: For isolation of human CD4+CD25+Foxp3+ regulatory T-cells (Treg cells), please use Dynabeads® Regulatory CD4+CD25+ T Cells (Cat. no. 113-63D).
Starting samples:
Whole blood, PBMC, buffy coat.
• Depletion of CD25+ T-cells
• Isolation of pure and viable CD25+ T-cells for molecular applications
• No columns required
Note: For isolation of human CD4+CD25+Foxp3+ regulatory T-cells (Treg cells), please use Dynabeads® Regulatory CD4+CD25+ T Cells (Cat. no. 113-63D).
Starting samples:
Whole blood, PBMC, buffy coat.
MagniSort™ Mouse CD8 Naïve T cell Enrichment Kit (Invitrogen™)
Le kit MagniSort™ Mouse CD8 Naive T cell Enrichment Kit est utilisé pour la séparation magnétique des cellules T CD8 naïves par sélection négative. Il a été optimisé pour isoler des cellules T CD8 naïves murines à partir de rates ou de ganglions lymphatiques de souris avec un mélange d’anticorps biotinylés et des microbilles magnétiques revêtues de streptavidine. Les cellules non souhaitées sont fixées aux anticorps, avant d’être immobilisées sur les microbilles magnétiques lorsqu’un champ magnétique est appliqué, laissant les cellules T CD8 naïves intactes et libres dans la solution.
Dynabeads™ FlowComp™ Human CD14 Kit (Invitrogen™)
Dynabeads® FlowComp™ Human CD14 contain magnetic beads that provide a robust and versatile tool to positively isolate pure CD14+ monocytes from whole blood, bone marrow, buffy coat, mononuclear cells (MNC), or tissue digests. The cells can be released from the beads using the FlowComp™ Release Buffer.
• Rapid isolation of CD14+ monocytes from any sample – no columns required
• Pure and bead-free CD14+ monocytes for any downstream application
Excellent Yield, Purity, and Viability of Recovered Cells
Dynabeads® FlowComp™ Human CD14 contains uniform, superparamagnetic beads (2.8 µm diameter) that enable easy isolation of human CD14+ monocytes directly from any sample via a three-step process. First, the FlowComp™ Human CD14 Antibody is mixed with the sample in a tube where it binds to the CD14+ monocytes during a short incubation. Then, the CD14+ monocytes that have bound the specific antibodies are captured by the FlowComp™ Dynabeads® and separated from the unbound cells using a magnet. Finally, the CD14+ monocytes are released from the beads by adding the FlowComp™ Release buffer. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity and viability of the isolated CD14+ monocytes.
Select what is right for you from our range of Human Monocyte Cell Isolation Products.
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
• Rapid isolation of CD14+ monocytes from any sample – no columns required
• Pure and bead-free CD14+ monocytes for any downstream application
Excellent Yield, Purity, and Viability of Recovered Cells
Dynabeads® FlowComp™ Human CD14 contains uniform, superparamagnetic beads (2.8 µm diameter) that enable easy isolation of human CD14+ monocytes directly from any sample via a three-step process. First, the FlowComp™ Human CD14 Antibody is mixed with the sample in a tube where it binds to the CD14+ monocytes during a short incubation. Then, the CD14+ monocytes that have bound the specific antibodies are captured by the FlowComp™ Dynabeads® and separated from the unbound cells using a magnet. Finally, the CD14+ monocytes are released from the beads by adding the FlowComp™ Release buffer. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity and viability of the isolated CD14+ monocytes.
Select what is right for you from our range of Human Monocyte Cell Isolation Products.
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
Dynabeads™ M-450 Tosylactivated (Invitrogen™)
Les microbilles Dynabeads® M-450 activées au tosyle sont des microbilles superparamagnétiques de 4,5 µm contenant des groupes de tosyle de surface. Les microbilles se lient de façon covalente aux groupes amines primaires et sulfhydryles des anticorps afin de les positionner dans l’orientation optimale pour la liaison aux protéines de surface. Grâce à un faible bruit de fond et une liaison covalente, ces microbilles constituent un excellent choix pour les applications de séparation de cellules lorsqu’une libération en aval des microbilles n’est pas nécessaire.
• Appauvrissement rapide des cellules ou isolement positif des cellules cibles pour une analyse moléculaire en aval
• Excellentes pour les procédures d’isolement des cellules à l’aide de votre propre anticorps unique
• Couplage de plusieurs anticorps possible sur les mêmes microbilles pour des applications qui ne sont pas basées sur des cellules, comme l’activation cellulaire
Procédure de couplage
Le couplage covalent s’effectue pendant la nuit en incubant l’anticorps de votre choix avec les microbilles Dynabeads® M-450 activées au tosyle. Le couplage optimal s’effectue à un pH élevé (8,5 à 9,5) et une température de 37°C. Pour les anticorps pH-labiles, le couplage peut être réalisé dans un tampon alternatif à pH 7,4.
Non recommandées pour l’immunoprécipitation (IP)
Ces microbilles de 4,5 µm activées au tosyle ne sont pas recommandées pour l’IP car elles présentent une capacité de surface par masse unitaire inférieure aux microbilles d’époxy Dynabeads® de 1 µm et 2,8 µm activées au tosyle.
En savoir plus sur les produits Dynabeads®
• Trouvez chez Dynabeads® les produits pour une gamme complète d’applications.
• Trouvez des aimants pour les séparations Dynabeads®.
• Appauvrissement rapide des cellules ou isolement positif des cellules cibles pour une analyse moléculaire en aval
• Excellentes pour les procédures d’isolement des cellules à l’aide de votre propre anticorps unique
• Couplage de plusieurs anticorps possible sur les mêmes microbilles pour des applications qui ne sont pas basées sur des cellules, comme l’activation cellulaire
Procédure de couplage
Le couplage covalent s’effectue pendant la nuit en incubant l’anticorps de votre choix avec les microbilles Dynabeads® M-450 activées au tosyle. Le couplage optimal s’effectue à un pH élevé (8,5 à 9,5) et une température de 37°C. Pour les anticorps pH-labiles, le couplage peut être réalisé dans un tampon alternatif à pH 7,4.
Non recommandées pour l’immunoprécipitation (IP)
Ces microbilles de 4,5 µm activées au tosyle ne sont pas recommandées pour l’IP car elles présentent une capacité de surface par masse unitaire inférieure aux microbilles d’époxy Dynabeads® de 1 µm et 2,8 µm activées au tosyle.
En savoir plus sur les produits Dynabeads®
• Trouvez chez Dynabeads® les produits pour une gamme complète d’applications.
• Trouvez des aimants pour les séparations Dynabeads®.
MagniSort™ Mouse CD49b Positive Selection Kit (Invitrogen™)
The MagniSort™ Mouse CD49b Positive Selection Kit is designed for the magnetic separation of CD49b+ cells by positive selection. It has been optimized for the isolation of CD49b+ cells from mouse spleens or lymph nodes utilizing a biotinylated Anti-Mouse CD49b antibody and streptavidin-coated magnetic beads. CD49b+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from CD49b+ cells by decanting.
After positive selection, the purity of selected cells can be verified by staining with Anti-Mouse CD49b (integrin alpha 2), clone DX5, and Anti-Mouse NK1.1, clone PK136. For mouse strains that do not express NK1.1 antigen such as BALB/c, SJL, AKR, CBA C3H, Anti-Mouse CD335 (NKp46), clone 29A1.4, may be used.
Reported Application
Magnetic Cell Separation
After positive selection, the purity of selected cells can be verified by staining with Anti-Mouse CD49b (integrin alpha 2), clone DX5, and Anti-Mouse NK1.1, clone PK136. For mouse strains that do not express NK1.1 antigen such as BALB/c, SJL, AKR, CBA C3H, Anti-Mouse CD335 (NKp46), clone 29A1.4, may be used.
Reported Application
Magnetic Cell Separation
