Shop All Secondary Detection Kits & Reagents
Human Dopaminergic Neuron Immunocytochemistry Kit (Invitrogen™)
The Human Dopaminergic Neuron Immunocytochemistry Kit enables optimal image-based analysis of three key markers of neural differentiation: OTX2, FoxA2, and tyrosine hydroxylase (TH). This high-performance immunocytochemistry (ICC) kit includes a complete set of primary and secondary antibodies, a nuclear DNA stain, and premade buffers for an optimized staining experiment.
The Human Dopaminergic Neuron Immunocytochemistry Kit enables you to:
• Confirm expression of key cellular markers with highly specific antibodies
• Get more information per sample by measuring more than one marker at a time
• Generate beautiful multiplexed images with minimal effort
• Save time and avoid cell loss by minimizing unnecessary wash steps in your staining protocol
• Process samples with confidence using a complete and optimized set of ICC reagents
Confirm expression of key cellular markers
The antibodies included in the kit have been carefully selected and validated for high performance in the ICC analysis of floor plate progenitors and dopaminergic neurons. OTX2 and FoxA2 are critical nuclear markers while TH is an important cytoplasmic marker.
Get more information per sample
The antibody combinations in the kit have been designed to allow for the specific and simultaneous assessment of two markers at a time (e.g., OTX2 and FoxA2, and FoxA2 and TH) along with nuclear DNA staining to help save time and precious sample.
Generate beautiful multiplexed images
Powered by the superior performance of bright, photostable Alexa Fluor™ dyes, this kit enables the acquisition of beautiful multiplexed images of key neural differentiation marker expression using commonly available blue, green, and orange/red filter sets.
Minimize unnecessary wash steps
The streamlined method recommended for this kit eliminates unnecessary wash steps found in standard ICC protocols, helping to save time and decrease the likelihood of cell loss during the staining protocol. The only washes required are after incubation with primary and secondary antibodies.
Process samples with confidence
An optimized set of premade fixation, permeabilization, blocking, and wash buffers allows you to focus on answering scientific questions rather than spending time preparing reagents and determining compatibility.
The Human Dopaminergic Neuron Immunocytochemistry Kit enables you to:
• Confirm expression of key cellular markers with highly specific antibodies
• Get more information per sample by measuring more than one marker at a time
• Generate beautiful multiplexed images with minimal effort
• Save time and avoid cell loss by minimizing unnecessary wash steps in your staining protocol
• Process samples with confidence using a complete and optimized set of ICC reagents
Confirm expression of key cellular markers
The antibodies included in the kit have been carefully selected and validated for high performance in the ICC analysis of floor plate progenitors and dopaminergic neurons. OTX2 and FoxA2 are critical nuclear markers while TH is an important cytoplasmic marker.
Get more information per sample
The antibody combinations in the kit have been designed to allow for the specific and simultaneous assessment of two markers at a time (e.g., OTX2 and FoxA2, and FoxA2 and TH) along with nuclear DNA staining to help save time and precious sample.
Generate beautiful multiplexed images
Powered by the superior performance of bright, photostable Alexa Fluor™ dyes, this kit enables the acquisition of beautiful multiplexed images of key neural differentiation marker expression using commonly available blue, green, and orange/red filter sets.
Minimize unnecessary wash steps
The streamlined method recommended for this kit eliminates unnecessary wash steps found in standard ICC protocols, helping to save time and decrease the likelihood of cell loss during the staining protocol. The only washes required are after incubation with primary and secondary antibodies.
Process samples with confidence
An optimized set of premade fixation, permeabilization, blocking, and wash buffers allows you to focus on answering scientific questions rather than spending time preparing reagents and determining compatibility.
Pierce™ Horseradish Peroxidase (Thermo Scientific™)
Thermo Scientific Pierce Horseradish Peroxidase (HRP) is purified horseradish peroxidase enzyme for use in activity assays and conjugation to antibodies for ELISA, Western blot and immunohistochemistry applications.
Features of Thermo Scientific Pierce Horseradish Peroxidase:
• Purified form—lyophilized salt-free powder; ready to dissolve and use
• High specific activity—typically greater than 300 units/mg (lot-specific value reported)
• Compared to AP—HRP is smaller (40kDa) than alkaline phosphatase (AP; 140kDa) and has higher specific enzyme activity than both AP and beta-galactosidase (b-Gal)
• Many options—numerous substrate solutions and assay techniques are available for HRP
This purified horseradish peroxidase (HRP) is supplied lyophilized as a salt-free powder for reconstitution and use in protein research methods. The main application for HRP in molecular biology and protein research is as a reporter system for immunoassays and other probe-based assay techniques such as ELISA, Western blotting, EMSA and Southern blotting. The enzyme is usually conjugated to specific secondary antibodies or streptavidin, and its activity is detected with a color-forming (or light-generating) substrate.
One unit catalyses the production of 1 mg of purpurogallin from pyrogallol in 20 seconds at 20°C and pH 6.0.
Features of Thermo Scientific Pierce Horseradish Peroxidase:
• Purified form—lyophilized salt-free powder; ready to dissolve and use
• High specific activity—typically greater than 300 units/mg (lot-specific value reported)
• Compared to AP—HRP is smaller (40kDa) than alkaline phosphatase (AP; 140kDa) and has higher specific enzyme activity than both AP and beta-galactosidase (b-Gal)
• Many options—numerous substrate solutions and assay techniques are available for HRP
This purified horseradish peroxidase (HRP) is supplied lyophilized as a salt-free powder for reconstitution and use in protein research methods. The main application for HRP in molecular biology and protein research is as a reporter system for immunoassays and other probe-based assay techniques such as ELISA, Western blotting, EMSA and Southern blotting. The enzyme is usually conjugated to specific secondary antibodies or streptavidin, and its activity is detected with a color-forming (or light-generating) substrate.
One unit catalyses the production of 1 mg of purpurogallin from pyrogallol in 20 seconds at 20°C and pH 6.0.
TRA-1-60 Alexa Fluor™ 555 Conjugate Kit for Live Cell Imaging (Invitrogen™)
The Molecular Probes® human pluripotent stem cell (PSC) live imaging kits are the only kits offering superior imaging for live human PSCs in one box. This TRA-1-60 Alexa Fluor® 555 Conjugate Kit includes an optimally labeled primary antibody paired with reduced-background imaging medium (FluoroBrite™ DMEM) for the specific detection of the PSC marker TRA-1-60. For fixed cell staining of PSC markers, we recommend the Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit.
With the TRA-1-60 Alexa Fluor® 555 Conjugate Kit for Live Cell Imaging, you can:
• Confirm the expression of a key pluripotent stem cell marker using an optimally dye-conjugated antibody
• Preserve cell health and maximize fluorescence signal with FluoroBrite™ DMEM imaging medium
• Stain live cell samples with confidence using reagents that are tested to be free of common contaminants
Confirm Expression of a Key PSC Marker
Powered by the high performance of bright, photostable Alexa Fluor® dyes, this kit enables reliable live-cell detection of the surface PSC marker TRA-1-60. This antibody conjugate has been demonstrated to show superior staining results when used with FluoroBrite™ DMEM imaging medium.
Preserve Cell Health and Maximize Signal
Staining cells in FluoroBrite™ DMEM imaging medium maximizes cell health and enhances signal-to-noise ratio when performing live-cell fluorescence imaging.
Stain Live-cell Samples with Confidence
Unlike most other conjugated antibodies, the reagents provided in this kit are 0.2 µm sterile-filtered and tested to be free of common contaminants.
What You Get
Easily scalable to any culture plate, dish, or slide format, the TRA-1-60 Alexa Fluor® 555 Conjugate Kit comes with reagents sufficient to stain 50 samples using a 200 uL staining volume and includes:
• 200 µL of Alexa Fluor® 555 Mouse Anti-Human TRA-1-60 (50X concentration)
• 500 mL FluoroBrite™ DMEM
With the TRA-1-60 Alexa Fluor® 555 Conjugate Kit for Live Cell Imaging, you can:
• Confirm the expression of a key pluripotent stem cell marker using an optimally dye-conjugated antibody
• Preserve cell health and maximize fluorescence signal with FluoroBrite™ DMEM imaging medium
• Stain live cell samples with confidence using reagents that are tested to be free of common contaminants
Confirm Expression of a Key PSC Marker
Powered by the high performance of bright, photostable Alexa Fluor® dyes, this kit enables reliable live-cell detection of the surface PSC marker TRA-1-60. This antibody conjugate has been demonstrated to show superior staining results when used with FluoroBrite™ DMEM imaging medium.
Preserve Cell Health and Maximize Signal
Staining cells in FluoroBrite™ DMEM imaging medium maximizes cell health and enhances signal-to-noise ratio when performing live-cell fluorescence imaging.
Stain Live-cell Samples with Confidence
Unlike most other conjugated antibodies, the reagents provided in this kit are 0.2 µm sterile-filtered and tested to be free of common contaminants.
What You Get
Easily scalable to any culture plate, dish, or slide format, the TRA-1-60 Alexa Fluor® 555 Conjugate Kit comes with reagents sufficient to stain 50 samples using a 200 uL staining volume and includes:
• 200 µL of Alexa Fluor® 555 Mouse Anti-Human TRA-1-60 (50X concentration)
• 500 mL FluoroBrite™ DMEM
TRA-1-60 Alexa Fluor™ 594 Conjugate Kit for Live Cell Imaging (Invitrogen™)
The Molecular Probes® human pluripotent stem cell (PSC) live imaging kits are the only kits offering superior imaging for live human PSCs in one box. This TRA-1-60 Alexa Fluor® 594 Conjugate Kit includes an optimally labeled primary antibody paired with reduced-background imaging medium (FluoroBrite™ DMEM) for the specific detection of the PSC marker TRA-1-60. For fixed cell staining of PSC markers, we recommend the Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit.
With the TRA-1-60 Alexa Fluor® 594 Conjugate Kit for Live Cell Imaging, you can:
• Confirm the expression of a key pluripotent stem cell marker using an optimally dye-conjugated antibody
• Preserve cell health and maximize fluorescence signal with FluoroBrite™ DMEM imaging medium
• Stain live cell samples with confidence using reagents that are tested to be free of common contaminants
Confirm Expression of a Key PSC Marker
Powered by the high performance of bright, photostable Alexa Fluor® dyes, this kit enables reliable live-cell detection of the surface PSC marker TRA-1-60. This antibody conjugate has been demonstrated to show superior staining results when used with FluoroBrite™ DMEM imaging medium.
Preserve Cell Health and Maximize Signal
Staining cells in FluoroBrite™ DMEM imaging medium maximizes cell health and enhances signal-to-noise ratio when performing live-cell fluorescence imaging.
Stain Live-cell Samples with Confidence
Unlike most other conjugated antibodies, the reagents provided in this kit are 0.2 µm sterile-filtered and tested to be free of common contaminants.
What You Get
Easily scalable to any culture plate, dish, or slide format, the TRA-1-60 Alexa Fluor® 594 Conjugate Kit comes with reagents sufficient to stain 50 samples using a 200 uL staining volume and includes:
• 200 µL of Alexa Fluor® 594 Mouse Anti-Human TRA-1-60 (50X concentration)
• 500 mL FluoroBrite™ DMEM
With the TRA-1-60 Alexa Fluor® 594 Conjugate Kit for Live Cell Imaging, you can:
• Confirm the expression of a key pluripotent stem cell marker using an optimally dye-conjugated antibody
• Preserve cell health and maximize fluorescence signal with FluoroBrite™ DMEM imaging medium
• Stain live cell samples with confidence using reagents that are tested to be free of common contaminants
Confirm Expression of a Key PSC Marker
Powered by the high performance of bright, photostable Alexa Fluor® dyes, this kit enables reliable live-cell detection of the surface PSC marker TRA-1-60. This antibody conjugate has been demonstrated to show superior staining results when used with FluoroBrite™ DMEM imaging medium.
Preserve Cell Health and Maximize Signal
Staining cells in FluoroBrite™ DMEM imaging medium maximizes cell health and enhances signal-to-noise ratio when performing live-cell fluorescence imaging.
Stain Live-cell Samples with Confidence
Unlike most other conjugated antibodies, the reagents provided in this kit are 0.2 µm sterile-filtered and tested to be free of common contaminants.
What You Get
Easily scalable to any culture plate, dish, or slide format, the TRA-1-60 Alexa Fluor® 594 Conjugate Kit comes with reagents sufficient to stain 50 samples using a 200 uL staining volume and includes:
• 200 µL of Alexa Fluor® 594 Mouse Anti-Human TRA-1-60 (50X concentration)
• 500 mL FluoroBrite™ DMEM
TSA™ Kit #15, with HRP—Goat Anti-Rabbit IgG and Alexa Fluor™ 594 Tyramide (Invitrogen™)
New SuperBoost™ Tyramide signal Amplification Kits offer 2-10 times the sensitivity of TSA™ kits. Explore the advantages of Alexa Fluor™ 594 Tyramide SuperBoost™ Kit - Goat anti-Rabbit IgG, which is replacing TSA™ Kit #15, with HRP--Goat Anti-Rabbit IgG and Alexa Fluor® 594 Tyramide.
Tyramide signal amplification is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid sequence in situ. The TSA Kit #15 includes HRP—goat anti-rabbit IgG and our bright, red-fluorescent Alexa Fluor 594 tyramide (which is spectrally similar to Texas Red dye).
Tyramide signal amplification is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid sequence in situ. The TSA Kit #15 includes HRP—goat anti-rabbit IgG and our bright, red-fluorescent Alexa Fluor 594 tyramide (which is spectrally similar to Texas Red dye).
Biotin XX Tyramide SuperBoost™ Kit, Goat anti-Mouse IgG (Invitrogen™)
SuperBoost™ tyramide signal amplification is the most sensitive method for detection of low abundant targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH). SuperBoost kits combine the brightness of AlexaFluor™ dyes with the superior signal amplification of a poly-HRP-mediated tyramide labeling reaction to produce a sensitivity 10-200 times greater than standard methods. SuperBoost kit sensitivity is also 2-10 times greater than regular tyramide amplification techniques like TSA™. For standout research, SuperBoost kits sharpen your results for clear visibility into critical areas that standard imaging methods fail to reveal.
SuperBoost kits are simple to use and easily adapted to standard ICC, IHC, or FISH experimental protocols, using any cell or tissue type. Cells labeled using a SuperBoost kit can be imaged using any type of microscope, producing high-resolution multiplex images. This particular kit features biotin XX tyramide with poly-HRP-conjugated goat anti-mouse IgG. For detection and additional signal amplification, conjugated streptavidin is used to detect specifically deposited biotin XX .
Features of the SuperBoost kits include:
• Superior sensitivity for detection of low-level or hard-to-detect targets by fluorescent imaging
• Simple protocol and detection using standard filters
• Suitable for high-resolution multiplex images—co-label with DAPI, secondary antibodies, and other SuperBoost kits
• Requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments
SuperBoost kits are based on the tyramide signal amplification system, which uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ. A typical ICC/IHC/ISH experiment using a SuperBoost kit requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments. SuperBoost kits offer superior specific signal intensity over background, so the protocol is easily optimized to detect specific signal in samples where high endogenous autofluorescence is observed.
Benefits of SuperBoost kits
Poly-HRP enhancement: Unlike TSA, SuperBoost kits employ poly-HRP-conjugated secondary antibodies. In such systems, several HRP enzymes are conjugated with short polymers, enhancing the signal by several fold over regular HRP systems. The poly-HRP is structured in such a way that the antibodies penetrate cells or tissue as efficiently as regular HRP-conjugated secondary antibodies. The molar enzyme/antibody protein ratio has an average value of '4'.
Reaction stop solution: Like any enzyme-based labeling system, it is possible to overdevelop the signal. SuperBoost kits include an HRP stop solution to halt the HRP reaction. HRP stop solution can be used to obtain maximum signal, without increase of background signal. Images produced with optimized HRP reaction times are as sharp as images produced with standard ICC/IHC/ISH methods, but with 10-200 times more sensitivity.
Reduction of background: SuperBoost kits include blockers for the elimination or reduction of endogenous peroxidase and fluorescent background signals. These blockers help ensure that only specific signals are enhanced while keeping non-specific/background signals in check.
SuperBoost kits are simple to use and easily adapted to standard ICC, IHC, or FISH experimental protocols, using any cell or tissue type. Cells labeled using a SuperBoost kit can be imaged using any type of microscope, producing high-resolution multiplex images. This particular kit features biotin XX tyramide with poly-HRP-conjugated goat anti-mouse IgG. For detection and additional signal amplification, conjugated streptavidin is used to detect specifically deposited biotin XX .
Features of the SuperBoost kits include:
• Superior sensitivity for detection of low-level or hard-to-detect targets by fluorescent imaging
• Simple protocol and detection using standard filters
• Suitable for high-resolution multiplex images—co-label with DAPI, secondary antibodies, and other SuperBoost kits
• Requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments
SuperBoost kits are based on the tyramide signal amplification system, which uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ. A typical ICC/IHC/ISH experiment using a SuperBoost kit requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments. SuperBoost kits offer superior specific signal intensity over background, so the protocol is easily optimized to detect specific signal in samples where high endogenous autofluorescence is observed.
Benefits of SuperBoost kits
Poly-HRP enhancement: Unlike TSA, SuperBoost kits employ poly-HRP-conjugated secondary antibodies. In such systems, several HRP enzymes are conjugated with short polymers, enhancing the signal by several fold over regular HRP systems. The poly-HRP is structured in such a way that the antibodies penetrate cells or tissue as efficiently as regular HRP-conjugated secondary antibodies. The molar enzyme/antibody protein ratio has an average value of '4'.
Reaction stop solution: Like any enzyme-based labeling system, it is possible to overdevelop the signal. SuperBoost kits include an HRP stop solution to halt the HRP reaction. HRP stop solution can be used to obtain maximum signal, without increase of background signal. Images produced with optimized HRP reaction times are as sharp as images produced with standard ICC/IHC/ISH methods, but with 10-200 times more sensitivity.
Reduction of background: SuperBoost kits include blockers for the elimination or reduction of endogenous peroxidase and fluorescent background signals. These blockers help ensure that only specific signals are enhanced while keeping non-specific/background signals in check.
Alexa Fluor™ 555 Tyramide SuperBoost™ Kit, streptavidin (Invitrogen™)
SuperBoost™ tyramide signal amplification is the most sensitive method for detection of low abundant targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH). SuperBoost kits combine the brightness of AlexaFluor™ dyes with the superior signal amplification of a poly-HRP-mediated tyramide labeling reaction to produce a sensitivity 10-200 times greater than standard methods. For standout research, SuperBoost kits sharpen your results for clear visibility into critical areas that standard imaging methods fail to reveal.
SuperBoost kits are simple to use and easily adapted to standard ICC, IHC, or FISH experimental protocols, using any cell or tissue type. Cells labeled using a SuperBoost kit can be imaged using any type of microscope, producing high-resolution multiplex images. This particular kit features AlexaFluor 555 tyramide (555/565 ex/em), detected using a standard Orange/RFP/TRITC filter cube. This kit also features HRP-conjugated streptavidin.
Features of the SuperBoost kits include:
• Superior sensitivity for detection of low-level or hard-to-detect targets by fluorescent imaging
• Simple protocol and detection using standard filters
• Suitable for high-resolution multiplex images—co-label with DAPI, secondary antibodies, and other SuperBoost kits
• Requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments
SuperBoost kits are based on the tyramide signal amplification system, which uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ. A typical ICC/IHC/ISH experiment using a SuperBoost kit requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments. SuperBoost kits offer superior specific signal intensity over background, so the protocol is easily optimized to detect specific signal in samples where high endogenous autofluorescence is observed.
Benefits of SuperBoost kits
Enhancement of signal using Alexa Fluor tyramides: SuperBoost kits utilize Alexa Fluor tyramides, which react with HRP to ultimately deposit bright and photostable Alexa Fluor dye on surrounding proteins and other similar molecules. SuperBoost kits are the only kits that combine the brightness of Alexa Fluor dyes with the enhancement of tyramide signal amplification to produce a superior signal.
Reaction stop solution: Like any enzyme-based labeling system, it is possible to overdevelop the signal. SuperBoost kits include an HRP stop solution to halt the HRP reaction. HRP stop solution can be used to obtain maximum signal, without increase of background signal. Images produced with optimized HRP reaction times are as sharp as images produced with standard ICC/IHC/ISH methods, but with 10-200 times more sensitivity.
Reduction of background: SuperBoost kits include blockers for the elimination or reduction of endogenous peroxidase and fluorescent background signals. These blockers help ensure that only specific signals are enhanced while keeping non-specific/background signals in check.
SuperBoost kits are simple to use and easily adapted to standard ICC, IHC, or FISH experimental protocols, using any cell or tissue type. Cells labeled using a SuperBoost kit can be imaged using any type of microscope, producing high-resolution multiplex images. This particular kit features AlexaFluor 555 tyramide (555/565 ex/em), detected using a standard Orange/RFP/TRITC filter cube. This kit also features HRP-conjugated streptavidin.
Features of the SuperBoost kits include:
• Superior sensitivity for detection of low-level or hard-to-detect targets by fluorescent imaging
• Simple protocol and detection using standard filters
• Suitable for high-resolution multiplex images—co-label with DAPI, secondary antibodies, and other SuperBoost kits
• Requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments
SuperBoost kits are based on the tyramide signal amplification system, which uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ. A typical ICC/IHC/ISH experiment using a SuperBoost kit requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments. SuperBoost kits offer superior specific signal intensity over background, so the protocol is easily optimized to detect specific signal in samples where high endogenous autofluorescence is observed.
Benefits of SuperBoost kits
Enhancement of signal using Alexa Fluor tyramides: SuperBoost kits utilize Alexa Fluor tyramides, which react with HRP to ultimately deposit bright and photostable Alexa Fluor dye on surrounding proteins and other similar molecules. SuperBoost kits are the only kits that combine the brightness of Alexa Fluor dyes with the enhancement of tyramide signal amplification to produce a superior signal.
Reaction stop solution: Like any enzyme-based labeling system, it is possible to overdevelop the signal. SuperBoost kits include an HRP stop solution to halt the HRP reaction. HRP stop solution can be used to obtain maximum signal, without increase of background signal. Images produced with optimized HRP reaction times are as sharp as images produced with standard ICC/IHC/ISH methods, but with 10-200 times more sensitivity.
Reduction of background: SuperBoost kits include blockers for the elimination or reduction of endogenous peroxidase and fluorescent background signals. These blockers help ensure that only specific signals are enhanced while keeping non-specific/background signals in check.
SuperBoost™ Goat anti-Rabbit Poly HRP (Invitrogen™)
SuperBoost™ Goat anti-Rabbit Poly HRP is a component in SuperBoost™ tyramide signal amplification kits made available separately here. SuperBoost™ Goat anti-Rabbit Poly HRP can be used with any HRP sustrate such as tyramide, DAB for signal amplification and detection. The molar enzyme/antibody protein ratio has an average value of '4'. This reagent is provided at a ready-to-use 1X concentration, enough for 150 slides.
When used as directed, this reagent with other SuperBoost reagents can be used for detection of low abundant targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH) with up to 200 times more sensitivity than standard methods. For standout research, SuperBoost reagents sharpen your results for clear visibility into critical areas that standard imaging methods fail to reveal. SuperBoost reagents are simple to use and easily adapted to standard ICC, IHC, or FISH experimental protocols, using any cell or tissue type. Cells labeled using a SuperBoost reagent can be imaged using any type of microscope, producing high-resolution multiplex images.
Features of the SuperBoost reagents include:
• Superior sensitivity for detection of low-level or hard-to-detect targets by fluorescent imaging
• Simple protocol and detection using standard filters
• Suitable for high-resolution multiplex images—co-label with TO-PRO™-3, secondary antibodies, and other SuperBoost kits
• Requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments
When used as directed, this reagent with other SuperBoost reagents can be used for detection of low abundant targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH) with up to 200 times more sensitivity than standard methods. For standout research, SuperBoost reagents sharpen your results for clear visibility into critical areas that standard imaging methods fail to reveal. SuperBoost reagents are simple to use and easily adapted to standard ICC, IHC, or FISH experimental protocols, using any cell or tissue type. Cells labeled using a SuperBoost reagent can be imaged using any type of microscope, producing high-resolution multiplex images.
Features of the SuperBoost reagents include:
• Superior sensitivity for detection of low-level or hard-to-detect targets by fluorescent imaging
• Simple protocol and detection using standard filters
• Suitable for high-resolution multiplex images—co-label with TO-PRO™-3, secondary antibodies, and other SuperBoost kits
• Requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments
Diaminobenzidine (DAB) Histochemistry Kit #3, with Streptavidin—HRP (Invitrogen™)
The DAB Histochemistry Kit for biotinylated probes contains sufficient materials to stain approximately 200 slides using horseradish peroxidase (HRP) and the HRP substrate DAB. The brown-colored DAB reaction product can be visualized by bright-fieldd light microscopy or, following osmication, by electron microscopy.
Alexa Fluor™ 647 Tyramide SuperBoost™ Kit, goat anti-rabbit IgG (Invitrogen™)
SuperBoost™ tyramide signal amplification is the most sensitive method for detection of low abundant targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH). SuperBoost kits combine the brightness of AlexaFluor™ dyes with the superior signal amplification of a poly-HRP-mediated tyramide labeling reaction to produce a sensitivity 10-200 times greater than standard methods. SuperBoost kit sensitivity is also 2-10 times greater than regular tyramide amplification techniques like TSA™. For standout research, SuperBoost kits sharpen your results for clear visibility into critical areas that standard imaging methods fail to reveal.
SuperBoost kits are simple to use and easily adapted to standard ICC, IHC, or FISH experimental protocols, using any cell or tissue type. Cells labeled using a SuperBoost kit can be imaged using any type of microscope, producing high-resolution multiplex images. This particular kit features AlexaFluor 647 tyramide (650/688 ex/em), detected using a standard Deep Red/Cy5 filter cube. This kit also features poly-HRP-conjugated goat anti-rabbit IgG secondary antibody.
Features of the SuperBoost kits include:
• Superior sensitivity for detection of low-level or hard-to-detect targets by fluorescent imaging
• Simple protocol and detection using standard filters
• Suitable for high-resolution multiplex images—co-label with DAPI, secondary antibodies, and other SuperBoost kits
• Requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments
SuperBoost kits are based on the tyramide signal amplification system, which uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ. A typical ICC/IHC/ISH experiment using a SuperBoost kit requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments. SuperBoost kits offer superior specific signal intensity over background, so the protocol is easily optimized to detect specific signal in samples where high endogenous autofluorescence is observed.
Benefits of SuperBoost kits
Enhancement of signal using Alexa Fluor tyramides: SuperBoost kits utilize Alexa Fluor tyramides, which react with HRP to ultimately deposit bright and photostable Alexa Fluor dye on surrounding proteins and other similar molecules. SuperBoost kits are the only kits that combine the brightness of Alexa Fluor dyes with the enhancement of tyramide signal amplification to produce a superior signal.
Poly-HRP enhancement: Unlike TSA, SuperBoost kits employ poly-HRP-conjugated secondary antibodies. In such systems, several HRP enzymes are conjugated with short polymers, enhancing the signal by several fold over regular HRP systems. The poly-HRP is structured in such a way that the antibodies penetrate cells or tissue as efficiently as regular HRP-conjugated secondary antibodies. The molar enzyme/antibody protein ratio has an average value of '4'.
Reaction stop solution: Like any enzyme-based labeling system, it is possible to overdevelop the signal. SuperBoost kits include an HRP stop solution to halt the HRP reaction. HRP stop solution can be used to obtain maximum signal, without increase of background signal. Images produced with optimized HRP reaction times are as sharp as images produced with standard ICC/IHC/ISH methods, but with 10-200 times more sensitivity.
Reduction of background: SuperBoost kits include blockers for the elimination or reduction of endogenous peroxidase and fluorescent background signals. These blockers help ensure that only specific signals are enhanced while keeping non-specific/background signals in check.
SuperBoost kits are simple to use and easily adapted to standard ICC, IHC, or FISH experimental protocols, using any cell or tissue type. Cells labeled using a SuperBoost kit can be imaged using any type of microscope, producing high-resolution multiplex images. This particular kit features AlexaFluor 647 tyramide (650/688 ex/em), detected using a standard Deep Red/Cy5 filter cube. This kit also features poly-HRP-conjugated goat anti-rabbit IgG secondary antibody.
Features of the SuperBoost kits include:
• Superior sensitivity for detection of low-level or hard-to-detect targets by fluorescent imaging
• Simple protocol and detection using standard filters
• Suitable for high-resolution multiplex images—co-label with DAPI, secondary antibodies, and other SuperBoost kits
• Requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments
SuperBoost kits are based on the tyramide signal amplification system, which uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ. A typical ICC/IHC/ISH experiment using a SuperBoost kit requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments. SuperBoost kits offer superior specific signal intensity over background, so the protocol is easily optimized to detect specific signal in samples where high endogenous autofluorescence is observed.
Benefits of SuperBoost kits
Enhancement of signal using Alexa Fluor tyramides: SuperBoost kits utilize Alexa Fluor tyramides, which react with HRP to ultimately deposit bright and photostable Alexa Fluor dye on surrounding proteins and other similar molecules. SuperBoost kits are the only kits that combine the brightness of Alexa Fluor dyes with the enhancement of tyramide signal amplification to produce a superior signal.
Poly-HRP enhancement: Unlike TSA, SuperBoost kits employ poly-HRP-conjugated secondary antibodies. In such systems, several HRP enzymes are conjugated with short polymers, enhancing the signal by several fold over regular HRP systems. The poly-HRP is structured in such a way that the antibodies penetrate cells or tissue as efficiently as regular HRP-conjugated secondary antibodies. The molar enzyme/antibody protein ratio has an average value of '4'.
Reaction stop solution: Like any enzyme-based labeling system, it is possible to overdevelop the signal. SuperBoost kits include an HRP stop solution to halt the HRP reaction. HRP stop solution can be used to obtain maximum signal, without increase of background signal. Images produced with optimized HRP reaction times are as sharp as images produced with standard ICC/IHC/ISH methods, but with 10-200 times more sensitivity.
Reduction of background: SuperBoost kits include blockers for the elimination or reduction of endogenous peroxidase and fluorescent background signals. These blockers help ensure that only specific signals are enhanced while keeping non-specific/background signals in check.
Alexa Fluor™ 594 Tyramide Reagent (Invitrogen™)
Alexa Fluor™ 594 Tyramide Reagent is a component of SuperBoost™ tyramide signal amplification kits made available separately here. This reagent can be used with any antibody conjugated to HRP for tyramide signal amplification for detection of low abundance targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH).
SuperBoost reagents, when used as directed, can increase sensitivity up to 200 times over standard imaging methods. For standout research, SuperBoost reagents sharpen your results for clear visibility into critical areas that standard imaging methods fail to reveal. SuperBoost reagents are simple to use and easily adapted to standard ICC, IHC, or FISH experimental protocols, using any cell or tissue type. Cells labeled using a SuperBoost reagent can be imaged using any type of microscope, producing high-resolution multiplex images. Alexa Fluor™ 594 Tyramide Reagent (591/617 ex/em) can be detected using a standard Texas Red™ filter cube.
Features of the SuperBoost reagents include:
• Superior sensitivity for detection of low-level or hard-to-detect targets by fluorescent imaging
• Simple protocol and detection using standard filters
• Suitable for high-resolution multiplex images—co-label with TO-PRO™-3, secondary antibodies, and other SuperBoost kits
• Requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments
SuperBoost reagents, when used as directed, can increase sensitivity up to 200 times over standard imaging methods. For standout research, SuperBoost reagents sharpen your results for clear visibility into critical areas that standard imaging methods fail to reveal. SuperBoost reagents are simple to use and easily adapted to standard ICC, IHC, or FISH experimental protocols, using any cell or tissue type. Cells labeled using a SuperBoost reagent can be imaged using any type of microscope, producing high-resolution multiplex images. Alexa Fluor™ 594 Tyramide Reagent (591/617 ex/em) can be detected using a standard Texas Red™ filter cube.
Features of the SuperBoost reagents include:
• Superior sensitivity for detection of low-level or hard-to-detect targets by fluorescent imaging
• Simple protocol and detection using standard filters
• Suitable for high-resolution multiplex images—co-label with TO-PRO™-3, secondary antibodies, and other SuperBoost kits
• Requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments
TSA™ Kit #21, with HRP—Streptavidin and Biotin-XX Tyramide (Invitrogen™)
New SuperBoost™ Tyramide signal Amplification Kits offer more sensitivity then TSA™ kits. Explore the advantages of Biotin XX Tyramide SuperBoost™ Kit - Streptavidin, which is replacing TSA™ Kit #21, with HRP--Streptavidin and Biotin-XX Tyramide.
Tyramide signal amplification is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid sequence in situ. The TSA Kit #21 includes HRP—streptavidin and biotin-XX tyramide. The deposited biotin molecules can be detected with any of our avidin, streptavidin or neutravidin conjugates.
Tyramide signal amplification is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid sequence in situ. The TSA Kit #21 includes HRP—streptavidin and biotin-XX tyramide. The deposited biotin molecules can be detected with any of our avidin, streptavidin or neutravidin conjugates.
Foxp3 Transcription Factor Staining Buffer Kit with Foxp3 Mouse Anti-Mouse mAb (clone 3G3), APC conjugate (Invitrogen™)
This product consists of a Foxp3 Transcription Factor Staining Buffer Kit (A24261) and a Foxp3 Mouse anti-Mouse mAb (clone 3G3), APC conjugate (A18629).
About the Antibody
The Foxp3 Mouse Anti-mouse mAb (clone 3G3), APC conjugate, is a monoclonal antibody that reacts with mouse Foxp3 protein. APC is a stable and highly soluble phycobiliprotein that provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus allowing for exceptional quantum yields and molar extinction coefficients. Use of APC in antibody conjugates results in probes with greatly enhanced detectability.
About the Buffers
The buffers in the included Foxp3 Transcription Factor Buffer Kit have been specially formulated to work with the APC conjugate of Foxp3 Mouse Anti-Mouse mAb (clone 3G3) for optimal observation of intranuclear staining when used in flow cytometry applications. The Foxp3 Transcription Factor Buffer Kit is compatible with intracellular cytokine staining within the same panel.
The kit contains 3 buffers:
• Foxp3 Fixation Transcription Factor and Permeabilization Buffer A (A24217, a 4X concentrate)
• Foxp3 Transcription Factor Fixation and Permeabilization Buffer B (A24218, a diluent)
• Foxp3 Transcription Factor Wash Buffer (A24261, 10X)
How the Buffers Work
The Foxp3 Transcription Factor Fixation and Permeabilization Buffer A is supplied as a 4X stock solution and must be diluted with Foxp3 Transcription Factor Fixation and Permeabilization Buffer B prior to use.
The Foxp3 Transcription Factor Wash Buffer is supplied as a 10X stock solution. The 10X stock solution should be diluted to a 1X working concentration in PBS containing 1% BSA and used for wash and intranuclear Foxp3 staining steps following fixation and permeabilization with 1X Foxp3 Fixation and Permabilization buffers.
About Foxp3
The 3G3 antibody reacts with mouse Foxp3 protein, a 50-55 kDa transcription factor that is a master regulator of regulatory T cell (Treg) development and function. Foxp3 is expressed constitutively by Tregs, which are further identified as being CD4+ CD25+, whereas resting conventional CD4+ T cells do not express Foxp3. However, TCR stimulation of naive T cells in the presence of TGF-beta has been shown to induce expression of Foxp3, driving their differentiation into Foxp3+ Tregs, which are called “induced" or “adaptive" Tregs. These cells are phenotypically similar to "natural" Tregs (CD4+ CD25+ Foxp3+), which originate in the thymus and comprise the majority of Tregs in the periphery. Tregs are critical for maintaining peripheral immune homeostasis. Defects in Foxp3 expression or Treg function have been implicated in the development of autoimmunity. Tregs have also been shown to restrict immune responses to infection, cancer, and vaccination. The 3G3 antibody may be used for intracellular detection of Foxp3 in cells from mouse and Rhesus macaque.
Related Links
Use the SpectraViewer to help you plan your experiments. Additional general information about Life Technologies® flow cytometry products and support is also available.
About the Antibody
The Foxp3 Mouse Anti-mouse mAb (clone 3G3), APC conjugate, is a monoclonal antibody that reacts with mouse Foxp3 protein. APC is a stable and highly soluble phycobiliprotein that provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus allowing for exceptional quantum yields and molar extinction coefficients. Use of APC in antibody conjugates results in probes with greatly enhanced detectability.
About the Buffers
The buffers in the included Foxp3 Transcription Factor Buffer Kit have been specially formulated to work with the APC conjugate of Foxp3 Mouse Anti-Mouse mAb (clone 3G3) for optimal observation of intranuclear staining when used in flow cytometry applications. The Foxp3 Transcription Factor Buffer Kit is compatible with intracellular cytokine staining within the same panel.
The kit contains 3 buffers:
• Foxp3 Fixation Transcription Factor and Permeabilization Buffer A (A24217, a 4X concentrate)
• Foxp3 Transcription Factor Fixation and Permeabilization Buffer B (A24218, a diluent)
• Foxp3 Transcription Factor Wash Buffer (A24261, 10X)
How the Buffers Work
The Foxp3 Transcription Factor Fixation and Permeabilization Buffer A is supplied as a 4X stock solution and must be diluted with Foxp3 Transcription Factor Fixation and Permeabilization Buffer B prior to use.
The Foxp3 Transcription Factor Wash Buffer is supplied as a 10X stock solution. The 10X stock solution should be diluted to a 1X working concentration in PBS containing 1% BSA and used for wash and intranuclear Foxp3 staining steps following fixation and permeabilization with 1X Foxp3 Fixation and Permabilization buffers.
About Foxp3
The 3G3 antibody reacts with mouse Foxp3 protein, a 50-55 kDa transcription factor that is a master regulator of regulatory T cell (Treg) development and function. Foxp3 is expressed constitutively by Tregs, which are further identified as being CD4+ CD25+, whereas resting conventional CD4+ T cells do not express Foxp3. However, TCR stimulation of naive T cells in the presence of TGF-beta has been shown to induce expression of Foxp3, driving their differentiation into Foxp3+ Tregs, which are called “induced" or “adaptive" Tregs. These cells are phenotypically similar to "natural" Tregs (CD4+ CD25+ Foxp3+), which originate in the thymus and comprise the majority of Tregs in the periphery. Tregs are critical for maintaining peripheral immune homeostasis. Defects in Foxp3 expression or Treg function have been implicated in the development of autoimmunity. Tregs have also been shown to restrict immune responses to infection, cancer, and vaccination. The 3G3 antibody may be used for intracellular detection of Foxp3 in cells from mouse and Rhesus macaque.
Related Links
Use the SpectraViewer to help you plan your experiments. Additional general information about Life Technologies® flow cytometry products and support is also available.
Alexa Fluor™ 488 Tyramide SuperBoost™ Kit, goat anti-mouse IgG (Invitrogen™)
SuperBoost™ tyramide signal amplification is the most sensitive method for detection of low abundant targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH). SuperBoost kits combine the brightness of AlexaFluor™ dyes with the superior signal amplification of a poly-HRP-mediated tyramide labeling reaction to produce a sensitivity 10-200 times greater than standard methods. SuperBoost kit sensitivity is also 2-10 times greater than regular tyramide amplification techniques like TSA™. For standout research, SuperBoost kits sharpen your results for clear visibility into critical areas that standard imaging methods fail to reveal.
SuperBoost kits are simple to use and easily adapted to standard ICC, IHC, or FISH experimental protocols, using any cell or tissue type. Cells labeled using a SuperBoost kit can be imaged using any type of microscope, producing high-resolution multiplex images. This particular kit features AlexaFluor 488 tyramide (496/524 ex/em), detected using a standard Green/FITC/GFP filter cube. This kit also features poly-HRP-conjugated goat anti-mouse IgG secondary antibody.
Features of the SuperBoost kits include:
• Superior sensitivity for detection of low-level or hard-to-detect targets by fluorescent imaging
• Simple protocol and detection using standard filters
• Suitable for high-resolution multiplex images—co-label with DAPI, secondary antibodies, and other SuperBoost kits
• Requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments
SuperBoost kits are based on the tyramide signal amplification system, which uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ. A typical ICC/IHC/ISH experiment using a SuperBoost kit requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments. SuperBoost kits offer superior specific signal intensity over background, so the protocol is easily optimized to detect specific signal in samples where high endogenous autofluorescence is observed.
Benefits of SuperBoost kits
Enhancement of signal using Alexa Fluor tyramides: SuperBoost kits utilize Alexa Fluor tyramides, which react with HRP to ultimately deposit bright and photostable Alexa Fluor dye on surrounding proteins and other similar molecules. SuperBoost kits are the only kits that combine the brightness of Alexa Fluor dyes with the enhancement of tyramide signal amplification to produce a superior signal.
Poly-HRP enhancement: Unlike TSA, SuperBoost kits employ poly-HRP-conjugated secondary antibodies. In such systems, several HRP enzymes are conjugated with short polymers, enhancing the signal by several fold over regular HRP systems. The poly-HRP is structured in such a way that the antibodies penetrate cells or tissue as efficiently as regular HRP-conjugated secondary antibodies. The molar enzyme/antibody protein ratio has an average value of '4'.
Reaction stop solution: Like any enzyme-based labeling system, it is possible to overdevelop the signal. SuperBoost kits include an HRP stop solution to halt the HRP reaction. HRP stop solution can be used to obtain maximum signal, without increase of background signal. Images produced with optimized HRP reaction times are as sharp as images produced with standard ICC/IHC/ISH methods, but with 10-200 times more sensitivity.
Reduction of background: SuperBoost kits include blockers for the elimination or reduction of endogenous peroxidase and fluorescent background signals. These blockers help ensure that only specific signals are enhanced while keeping non-specific/background signals in check.
SuperBoost kits are simple to use and easily adapted to standard ICC, IHC, or FISH experimental protocols, using any cell or tissue type. Cells labeled using a SuperBoost kit can be imaged using any type of microscope, producing high-resolution multiplex images. This particular kit features AlexaFluor 488 tyramide (496/524 ex/em), detected using a standard Green/FITC/GFP filter cube. This kit also features poly-HRP-conjugated goat anti-mouse IgG secondary antibody.
Features of the SuperBoost kits include:
• Superior sensitivity for detection of low-level or hard-to-detect targets by fluorescent imaging
• Simple protocol and detection using standard filters
• Suitable for high-resolution multiplex images—co-label with DAPI, secondary antibodies, and other SuperBoost kits
• Requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments
SuperBoost kits are based on the tyramide signal amplification system, which uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ. A typical ICC/IHC/ISH experiment using a SuperBoost kit requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments. SuperBoost kits offer superior specific signal intensity over background, so the protocol is easily optimized to detect specific signal in samples where high endogenous autofluorescence is observed.
Benefits of SuperBoost kits
Enhancement of signal using Alexa Fluor tyramides: SuperBoost kits utilize Alexa Fluor tyramides, which react with HRP to ultimately deposit bright and photostable Alexa Fluor dye on surrounding proteins and other similar molecules. SuperBoost kits are the only kits that combine the brightness of Alexa Fluor dyes with the enhancement of tyramide signal amplification to produce a superior signal.
Poly-HRP enhancement: Unlike TSA, SuperBoost kits employ poly-HRP-conjugated secondary antibodies. In such systems, several HRP enzymes are conjugated with short polymers, enhancing the signal by several fold over regular HRP systems. The poly-HRP is structured in such a way that the antibodies penetrate cells or tissue as efficiently as regular HRP-conjugated secondary antibodies. The molar enzyme/antibody protein ratio has an average value of '4'.
Reaction stop solution: Like any enzyme-based labeling system, it is possible to overdevelop the signal. SuperBoost kits include an HRP stop solution to halt the HRP reaction. HRP stop solution can be used to obtain maximum signal, without increase of background signal. Images produced with optimized HRP reaction times are as sharp as images produced with standard ICC/IHC/ISH methods, but with 10-200 times more sensitivity.
Reduction of background: SuperBoost kits include blockers for the elimination or reduction of endogenous peroxidase and fluorescent background signals. These blockers help ensure that only specific signals are enhanced while keeping non-specific/background signals in check.
Horseradish Peroxidase (HRP) (EIA Grade) (Invitrogen™)
This highly purified preparation of horseradish peroxidase is useful as a protein tracer in histochemistry as well as cytochemistry, and also as a valuable experimental tool in hodological neurography. Also, the enzyme preparation has been used as an enzyme label in enzyme immunoassays.
