Shop All Microbiological Media
imMedia™ Growth Medium, agar, kanamycin (Invitrogen™)
imMedia Growth Medium is pre-mixed, pre-sterilized E. coli growth medium that can be prepared in just five minutes without autoclaving. imMedia Growth Medium contains everything you need to prepare low-salt LB agar plates. You'll save hours of medium preparation time because there is:
• No weighing or mixing of media components
• No autoclaving
• No waiting for media to cool before adding antibiotics
Fast medium preparation
imMedia Growth Medium is fast food for E. coli. Simply mix with water in a clean flask, heat in a microwave oven, and in less than 5 minutes your medium is prepared.
The ultimate convenience
For your convenience, imMedia Growth Medium is available for preparing liquid medium or agar plates, with or without X-gal/IPTG. You have a choice of two antibiotics: ampicillin and kanamycin. imMedia Growth Medium is packaged in ready-to-use individual pouches. Each pouch contains sufficient reagents for preparing 200 mL of liquid media or 8-10 standard 100 mm2 agar plates.
Quality results
Each type of imMedia Growth Medium is extensively tested to ensure E. coli growth, antibiotic activity, and sterility. imMedia "Blue" products (with X-gal/IPTG) are tested for efficient blue/white colony screening.
• No weighing or mixing of media components
• No autoclaving
• No waiting for media to cool before adding antibiotics
Fast medium preparation
imMedia Growth Medium is fast food for E. coli. Simply mix with water in a clean flask, heat in a microwave oven, and in less than 5 minutes your medium is prepared.
The ultimate convenience
For your convenience, imMedia Growth Medium is available for preparing liquid medium or agar plates, with or without X-gal/IPTG. You have a choice of two antibiotics: ampicillin and kanamycin. imMedia Growth Medium is packaged in ready-to-use individual pouches. Each pouch contains sufficient reagents for preparing 200 mL of liquid media or 8-10 standard 100 mm2 agar plates.
Quality results
Each type of imMedia Growth Medium is extensively tested to ensure E. coli growth, antibiotic activity, and sterility. imMedia "Blue" products (with X-gal/IPTG) are tested for efficient blue/white colony screening.
MagicMedia™ E. coli Expression Medium (Invitrogen™)
MagicMedia™ E. coli Expression Medium is specifically designed to dramatically increase the yield of recombinant proteins in T7-regulated E. coli expression systems without monitoring culture O.D. (optical densities) or adding inducer. The proprietary MagicMedia™ formulation enables E. coli growth to reach culture densities 3-10-fold higher than those achieved with traditional LB + IPTG methods, allowing you to:
• Achieve 3-10 times higher protein yields (Figure 1) with much less hands-on time
• Eliminate time-consuming O.D. monitoring steps-simply inoculate prepared MagicMedia™ E. coli Expression Medium, grow the culture overnight, and harvest (Figure 2)
• Reduce culture volume to simplify handling and scale-up processes
In addition, MagicMedia™ E. coli Expression Medium is ideal for simultaneous expression of multiple clones for high-throughput applications.
Content and Storage:
MagicMedia™ E. coli Expression Medium is available in two convenient formats to meet specific needs-premixed powder packaged in a convenient, dissolvable bag (SoluPouch™ format) or a ready-to-use, presterilized liquid. Formats contain proprietary component A (in SoluPouch™ powder or 1X liquid format) and component B (in buffer format). Store the SoluPouch™ format at room temperature. Store the liquid format at +4°C. All components are guaranteed stable for 6 months when properly stored.
• Achieve 3-10 times higher protein yields (Figure 1) with much less hands-on time
• Eliminate time-consuming O.D. monitoring steps-simply inoculate prepared MagicMedia™ E. coli Expression Medium, grow the culture overnight, and harvest (Figure 2)
• Reduce culture volume to simplify handling and scale-up processes
In addition, MagicMedia™ E. coli Expression Medium is ideal for simultaneous expression of multiple clones for high-throughput applications.
Content and Storage:
MagicMedia™ E. coli Expression Medium is available in two convenient formats to meet specific needs-premixed powder packaged in a convenient, dissolvable bag (SoluPouch™ format) or a ready-to-use, presterilized liquid. Formats contain proprietary component A (in SoluPouch™ powder or 1X liquid format) and component B (in buffer format). Store the SoluPouch™ format at room temperature. Store the liquid format at +4°C. All components are guaranteed stable for 6 months when properly stored.
LB Broth (Invitrogen™)
Le bouillon LB (1X) est un milieu de croissance liquide prêt à l’emploi utilisé pour la culture bactérienne. Notre bouillon LB stérilisé par filtration peut être utilisé pour presque toutes les applications qui nécessitent une étape de mise en culture bactérienne liquide, comme l’expression, le clonage et l’expansion de protéines.
Formulation du bouillon LB
Le bouillon LB est l’un des milieux bactériens les plus référencés pour cultiver et maintenir des souches d’Escherichia coli (E. coli). Notre bouillon LB est basé sur la formulation Lennox, l’une des formulations les plus couramment utilisées pour le milieu LB. Formule pour 1 litre : 10 g de peptone SELECT 140, 5 g d’extrait de levure SELECT, 5 g de chlorure de sodium.
Prêt à l’emploi dans des flacons pour milieu de culture Gibco® optimisés
Chaque flacon de bouillon LB est stérilisé par filtration et prêt à l’emploi dans des flacons pour milieu de culture Gibco® ergonomiques possédant des ouvertures inclinées pour un pipetage simplifié.
Formulation du bouillon LB
Le bouillon LB est l’un des milieux bactériens les plus référencés pour cultiver et maintenir des souches d’Escherichia coli (E. coli). Notre bouillon LB est basé sur la formulation Lennox, l’une des formulations les plus couramment utilisées pour le milieu LB. Formule pour 1 litre : 10 g de peptone SELECT 140, 5 g d’extrait de levure SELECT, 5 g de chlorure de sodium.
Prêt à l’emploi dans des flacons pour milieu de culture Gibco® optimisés
Chaque flacon de bouillon LB est stérilisé par filtration et prêt à l’emploi dans des flacons pour milieu de culture Gibco® ergonomiques possédant des ouvertures inclinées pour un pipetage simplifié.
Terrific Broth (Invitrogen™)
Formulation per one liter:
11.8 g SELECT Peptone 140
23.6 g Yeast Extract
9.4 g dipotassium hydrogen phosphate
2.2 g potassium dihydrogen phosphate
11.8 g SELECT Peptone 140
23.6 g Yeast Extract
9.4 g dipotassium hydrogen phosphate
2.2 g potassium dihydrogen phosphate
MagicMedia™ E. coli Expression Medium, Powder (Invitrogen™)
MagicMedia™ E. coli Expression Medium is specifically designed to dramatically increase the yield of recombinant proteins in T7-regulated E. coli expression systems without monitoring culture OD (optical density) or adding inducer. The proprietary MagicMedia™ formulation enables E. coli growth to reach culture densities 3–10-fold higher than those achieved with traditional LB + IPTG methods, allowing you to:
• Achieve 3–10 times higher protein yields (Figure 1) with much less hands-on time
• Eliminate time-consuming OD monitoring steps—simply inoculate, grow the culture overnight, and harvest (Figure 2)
• Reduce culture volume to simplify handling and scale-up processes
In addition, MagicMedia™ E. coli Expression Medium is ideal for simultaneous expression of multiple clones for high-throughput applications.
MagicMedia™ E. coli Expression Medium is available in two convenient formats to meet specific needs: premixed powder packaged in a convenient, dissolvable bag (foil pouch format) or a ready-to-use, pre-sterilized liquid. The formats contain proprietary component A (in foil pouch powder or 1X liquid format) and component B (in buffer format).
• Achieve 3–10 times higher protein yields (Figure 1) with much less hands-on time
• Eliminate time-consuming OD monitoring steps—simply inoculate, grow the culture overnight, and harvest (Figure 2)
• Reduce culture volume to simplify handling and scale-up processes
In addition, MagicMedia™ E. coli Expression Medium is ideal for simultaneous expression of multiple clones for high-throughput applications.
MagicMedia™ E. coli Expression Medium is available in two convenient formats to meet specific needs: premixed powder packaged in a convenient, dissolvable bag (foil pouch format) or a ready-to-use, pre-sterilized liquid. The formats contain proprietary component A (in foil pouch powder or 1X liquid format) and component B (in buffer format).
2-YT Broth (Invitrogen™)
Application(s): For growth of hosts for replication of M13 vectors.
Instructions for use:
1. Suspend 31 g in 1 L of demineralized water.
2. Autoclave for 15 min at 121°C.
Instructions for use:
1. Suspend 31 g in 1 L of demineralized water.
2. Autoclave for 15 min at 121°C.
S.c. EasyComp™ Transformation Kit (Invitrogen™)
The S.c. EasyComp™ Transformation Kit is designed for rapid preparation of transformation-competent Saccharomyces cerevisiae cells. Advantages over commonly used transformation procedures such as spheroplast formation or LiCl methods include:
• Cells can be made competent in less than 30 minutes
• Competent cell preparation and transformation solutions are optimized for use with S. cerevisiae
are provided ready-to-use and tested to ensure successful yeast transformation
• Competent cells can be frozen for future use
• Cells can be made competent in less than 30 minutes
• Competent cell preparation and transformation solutions are optimized for use with S. cerevisiae
are provided ready-to-use and tested to ensure successful yeast transformation
• Competent cells can be frozen for future use
LB Broth Base (Invitrogen™)
LB Broth Base (Lennox L Broth Base) is recommended for the general maintenance and growth of E. coli.
LB Broth Base formulation
LB Broth Base has a lower salt concentration than Luria Broth Base. The formulation for 1 liter is: 10 g SELECT Peptone 140, 5 g SELECT Yeast Extract, and 5 g NaCl.
LB Broth Base formulation
LB Broth Base has a lower salt concentration than Luria Broth Base. The formulation for 1 liter is: 10 g SELECT Peptone 140, 5 g SELECT Yeast Extract, and 5 g NaCl.
CT Supplement (Applied Biosystems™)
CT Supplement is added to standard Sorbitol MacConkey media (SMAC) to form CT-SMAC. CT-SMAC selects for E.coli 0157 after IMS.
This Product Contains: 1mg Cefixime powder and 50mg Potassium Tellurite powder.
This Product Contains: 1mg Cefixime powder and 50mg Potassium Tellurite powder.
imMedia™ Growth Medium, agar, ampicillin (Invitrogen™)
imMedia Growth Medium is pre-mixed, pre-sterilized E. coli growth medium that can be prepared in just five minutes without autoclaving. imMedia Growth Medium contains everything you need to prepare low-salt LB agar plates. You'll save hours of medium preparation time because there is:
• No weighing or mixing of media components
• No autoclaving
• No waiting for media to cool before adding antibiotics
Fast medium preparation
imMedia Growth Medium is fast food for E. coli. Simply mix with water in a clean flask, heat in a microwave oven, and in less than 5 minutes your medium is prepared.
The ultimate convenience
For your convenience, imMedia Growth Medium is available for preparing liquid medium or agar plates, with or without X-gal/IPTG. You have a choice of two antibiotics: ampicillin and kanamycin. imMedia Growth Medium is packaged in ready-to-use individual pouches. Each pouch contains sufficient reagents for preparing 200 mL of liquid media or 8-10 standard 100 mm2 agar plates.
Quality results
Each type of imMedia Growth Medium is extensively tested to ensure E. coli growth, antibiotic activity, and sterility. imMedia "Blue" products (with X-gal/IPTG) are tested for efficient blue/white colony screening.
• No weighing or mixing of media components
• No autoclaving
• No waiting for media to cool before adding antibiotics
Fast medium preparation
imMedia Growth Medium is fast food for E. coli. Simply mix with water in a clean flask, heat in a microwave oven, and in less than 5 minutes your medium is prepared.
The ultimate convenience
For your convenience, imMedia Growth Medium is available for preparing liquid medium or agar plates, with or without X-gal/IPTG. You have a choice of two antibiotics: ampicillin and kanamycin. imMedia Growth Medium is packaged in ready-to-use individual pouches. Each pouch contains sufficient reagents for preparing 200 mL of liquid media or 8-10 standard 100 mm2 agar plates.
Quality results
Each type of imMedia Growth Medium is extensively tested to ensure E. coli growth, antibiotic activity, and sterility. imMedia "Blue" products (with X-gal/IPTG) are tested for efficient blue/white colony screening.
LB Agar, powder (Lennox L agar) (Invitrogen™)
Formule pour 1 litre :
10 g de peptone SELECT 140
5 g d’extrait de levure SELECT
5 g de chlorure de sodium
Gélose SELECT 12 g
10 g de peptone SELECT 140
5 g d’extrait de levure SELECT
5 g de chlorure de sodium
Gélose SELECT 12 g
CSM Media for Mav203 Yeast Cells (Invitrogen™)
The CSM Media for Mav203 Yeast Cells is a specifically designed media for making agar plates that enable the growth of Saccharomyces cerevisae MaV203 cells. This product comes with enough reagents to prepare 2 liters of solid media with the following components per liter:
• CSM (without tryptophan): 0.74 g
• Yeast Nitrogen Base: 6.66 g
• Ammonium Sulfate: 5 g
• Glucose: 20 g
• Agar: 20 g
Compatible with All MaV203 Applications
The CSM Media is optimized for the GeneArt® High-Order Genetic Assembly System, including the recovery for transformations of vectors once they have been adapted to yeast using the GeneArt® High-Order Vector Conversion Cassette. The media can also be used to grow MaV203 competent yeast cells, both library and sub-cloning scale (cat# 11281-011 and 11445-012).
Plate Preparation Protocol
For each liter, use one pouch of CSM -Trp Agar Yeast Media minus Glucose and 1 bottle of 20% Glucose. Dissolve the contents of the pouch in 900ml of distilled water and autoclave for 20 minutes on liquid cycle. Allow the solution to cool to 55°C and then add one bottle of 20% Glucose (100ml). Mix well and pour plates.
• CSM (without tryptophan): 0.74 g
• Yeast Nitrogen Base: 6.66 g
• Ammonium Sulfate: 5 g
• Glucose: 20 g
• Agar: 20 g
Compatible with All MaV203 Applications
The CSM Media is optimized for the GeneArt® High-Order Genetic Assembly System, including the recovery for transformations of vectors once they have been adapted to yeast using the GeneArt® High-Order Vector Conversion Cassette. The media can also be used to grow MaV203 competent yeast cells, both library and sub-cloning scale (cat# 11281-011 and 11445-012).
Plate Preparation Protocol
For each liter, use one pouch of CSM -Trp Agar Yeast Media minus Glucose and 1 bottle of 20% Glucose. Dissolve the contents of the pouch in 900ml of distilled water and autoclave for 20 minutes on liquid cycle. Allow the solution to cool to 55°C and then add one bottle of 20% Glucose (100ml). Mix well and pour plates.
YPD Broth (Gibco™)
Gibco® YPD Broth is a ready-to-use liquid growth media used for yeast culture. Our filter-sterilized Gibco® YPD Broth can be used for nearly all research applications that require a liquid yeast culturing step such as protein expression, cloning, and expansion.
• Gibco® microbiological media
• Convenient ready-to-use liquid format
• 1 L size available
YPD Broth Recipe
YPD Broth is a commonly used yeast growth media for maintenance and propagation of Pichia pastoris (P. pastoris) and Saccharomyces cerevisae (S. cerevisae) yeast strains.
Formulation per One Liter:
10 g Yeast extract
20 g Bacto peptone
20 g Dextrose (glucose)
Ready-to-Use in Optimized Gibco® Media Bottles
Each bottle of Gibco® YPD Broth is filter-sterilized and ready-to-use in ergonomic Gibco® media bottles with angled openings for simplified pipetting.
For research use only. Not intended for human or animal therapeutic or diagnostic use.
• Gibco® microbiological media
• Convenient ready-to-use liquid format
• 1 L size available
YPD Broth Recipe
YPD Broth is a commonly used yeast growth media for maintenance and propagation of Pichia pastoris (P. pastoris) and Saccharomyces cerevisae (S. cerevisae) yeast strains.
Formulation per One Liter:
10 g Yeast extract
20 g Bacto peptone
20 g Dextrose (glucose)
Ready-to-Use in Optimized Gibco® Media Bottles
Each bottle of Gibco® YPD Broth is filter-sterilized and ready-to-use in ergonomic Gibco® media bottles with angled openings for simplified pipetting.
For research use only. Not intended for human or animal therapeutic or diagnostic use.
imMedia™ Growth Medium, liquid, kanamycin (Invitrogen™)
imMedia Growth Medium is pre-mixed, pre-sterilized E. coli growth medium that can be prepared in just five minutes without autoclaving. imMedia Growth Medium contains everything you need to prepare low-salt LB liquid medium. You'll save hours of medium preparation time because there is:
• No weighing or mixing of media components
• No autoclaving
• No waiting for media to cool before adding antibiotics
Fast medium preparation
imMedia Growth Medium is fast food for E. coli. Simply mix with water in a clean flask, heat in a microwave oven, and in less than 5 minutes your medium is prepared.
The ultimate convenience
For your convenience, imMedia Growth Medium is available for preparing liquid medium or agar plates, with or without X-gal/IPTG. You have a choice of two antibiotics: ampicillin and kanamycin. imMedia Growth Medium is packaged in ready-to-use individual pouches. Each pouch contains sufficient reagents for preparing 200 mL of liquid media or 8-10 standard 100 mm2 agar plates.
Quality results
Each type of imMedia Growth Medium is extensively tested to ensure E. coli growth, antibiotic activity, and sterility. imMedia "Blue" products (with X-gal/IPTG) are tested for efficient blue/white colony screening.
• No weighing or mixing of media components
• No autoclaving
• No waiting for media to cool before adding antibiotics
Fast medium preparation
imMedia Growth Medium is fast food for E. coli. Simply mix with water in a clean flask, heat in a microwave oven, and in less than 5 minutes your medium is prepared.
The ultimate convenience
For your convenience, imMedia Growth Medium is available for preparing liquid medium or agar plates, with or without X-gal/IPTG. You have a choice of two antibiotics: ampicillin and kanamycin. imMedia Growth Medium is packaged in ready-to-use individual pouches. Each pouch contains sufficient reagents for preparing 200 mL of liquid media or 8-10 standard 100 mm2 agar plates.
Quality results
Each type of imMedia Growth Medium is extensively tested to ensure E. coli growth, antibiotic activity, and sterility. imMedia "Blue" products (with X-gal/IPTG) are tested for efficient blue/white colony screening.
