Shop All Transfection Reagents
Lipofectamine™ 2000 CD Transfection Reagent (Invitrogen™)
Le réactif de transfection Lipofectamine® 2000 CD (défini chimiquement) est une formulation exclusive sans composants d’origine animale permettant la transfection des acides nucléiques dans les cellules eucaryotes. Un fichier maître des médicaments (DMF) a été soumis à la FDA. Contactez le service technique ou votre représentant commercial le plus proche pour obtenir l’autorisation de renvoi au DMF. L’utilisation du réactif Lipofectamine® 2000 CD pour la transfection offre les avantages suivants :
• Plus haute efficacité de transfection dans de nombreux types et formats cellulaires
• Formulation sans composants d’origine animale, ce qui garantit que la culture de cellules de mammifères et les processus de bioproduction sont exempts de matières dérivées d’animaux.
• Des complexes DNA-Lipofectamine® 2000 CD peuvent être directement ajoutés aux cellules dans le milieu de culture
• Il n’est pas nécessaire d’éliminer les complexes ou de changer /ajouter du milieu après transfection, mais les complexes peuvent être éliminés après 4 à 6 heures
Large gamme de réactifs de transfection
Notre gamme étendue de réactifs de transfection offre des hautes performances constantes et fiables. Ces réactifs permettent la transfection hautement efficace d’une large gamme de types cellulaires, y compris les lignées adhérentes, en suspension, d’insectes et endothéliales, ainsi que les cultures primaires. Tous les réactifs de transfection comprennent des protocoles rationalisés pour un maximum de confort et de simplicité d’utilisation dans de nombreuses applications, y compris les applications à haut débit.
• Plus haute efficacité de transfection dans de nombreux types et formats cellulaires
• Formulation sans composants d’origine animale, ce qui garantit que la culture de cellules de mammifères et les processus de bioproduction sont exempts de matières dérivées d’animaux.
• Des complexes DNA-Lipofectamine® 2000 CD peuvent être directement ajoutés aux cellules dans le milieu de culture
• Il n’est pas nécessaire d’éliminer les complexes ou de changer /ajouter du milieu après transfection, mais les complexes peuvent être éliminés après 4 à 6 heures
Large gamme de réactifs de transfection
Notre gamme étendue de réactifs de transfection offre des hautes performances constantes et fiables. Ces réactifs permettent la transfection hautement efficace d’une large gamme de types cellulaires, y compris les lignées adhérentes, en suspension, d’insectes et endothéliales, ainsi que les cultures primaires. Tous les réactifs de transfection comprennent des protocoles rationalisés pour un maximum de confort et de simplicité d’utilisation dans de nombreuses applications, y compris les applications à haut débit.
Lipofectamine™ RNAiMAX Transfection Reagent (Invitrogen™)
Le réactif de transfection Lipofectamine® ARNiMAX offre une efficacité de transfection exceptionnelle dans une très large gamme de types cellulaires pour les expériences d’extinction de gènes utilisant l’ARNic. Lipofectamine® ARNiMAX est une formulation exclusive de lipides cationiques propre à l’ARNi et conçue spécialement pour l’administration du ARNic et du micro-ARN dans tout type de cellule.
Grâce au réactif de transfection Lipofectamine® RNAiMAX, vous obtiendrez :
• une efficacité de transfection exceptionnelle exigeant des concentrations inférieures d’ARNi, ce qui se solde par une efficacité accrue de l’expérience d’extinction de gènes, avec un minimum d’effets non spécifiques ;
• une optimisation aisée compte tenu de la cytotoxicité minimale sur une plage de concentration 10 fois supérieure du réactif de transfection ;
• une efficacité de transfection exceptionnelle pour les antagonistes et les mimiques du micro-ARN ;
• une compatibilité avec une large gamme de types cellulaires, avec une approche ultra polyvalente pour toutes vos expériences d’inhibition génétique ;
• un protocole simple et rapide pour des résultats uniformes et reproductibles.
Extinction élevée dans une large gamme de cellules
Le réactif de transfection Lipofectamine® RNAiMAX procède à la transfection d’une large gamme de types de cellules (voir la figure). Pour l’inhibition génétique, les transfections hautement efficaces du réactif de transfection Lipofectamine® RNAiMAX permettent des expériences d’extinction de gènes de haut niveau, nécessaires à la production de résultats convaincants.
Transfections simples, prêtes à l’emploi, à haut débit
Il vous suffit de mélanger le réactif de transfection Lipofectamine® RNAiMAX avec l’ARNic, de l’ajouter aux cellules, de l’incuber et de mesurer l’extinction des gènes. La simplicité et la vitesse combinées à une efficacité exceptionnelle de transfection rendent le réactif de transfection Lipofectamine® RNAiMAX idéal pour les transfections d’ARNic à haut débit. Les conditions de transfection peuvent facilement être établies pour les systèmes robotique ou automatisés utilisés dans de telles applications.
Trouvez un protocole de transfection optimisé Lipofectamine® RNAiMAX pour votre lignée cellulaire.
Grâce au réactif de transfection Lipofectamine® RNAiMAX, vous obtiendrez :
• une efficacité de transfection exceptionnelle exigeant des concentrations inférieures d’ARNi, ce qui se solde par une efficacité accrue de l’expérience d’extinction de gènes, avec un minimum d’effets non spécifiques ;
• une optimisation aisée compte tenu de la cytotoxicité minimale sur une plage de concentration 10 fois supérieure du réactif de transfection ;
• une efficacité de transfection exceptionnelle pour les antagonistes et les mimiques du micro-ARN ;
• une compatibilité avec une large gamme de types cellulaires, avec une approche ultra polyvalente pour toutes vos expériences d’inhibition génétique ;
• un protocole simple et rapide pour des résultats uniformes et reproductibles.
Extinction élevée dans une large gamme de cellules
Le réactif de transfection Lipofectamine® RNAiMAX procède à la transfection d’une large gamme de types de cellules (voir la figure). Pour l’inhibition génétique, les transfections hautement efficaces du réactif de transfection Lipofectamine® RNAiMAX permettent des expériences d’extinction de gènes de haut niveau, nécessaires à la production de résultats convaincants.
Transfections simples, prêtes à l’emploi, à haut débit
Il vous suffit de mélanger le réactif de transfection Lipofectamine® RNAiMAX avec l’ARNic, de l’ajouter aux cellules, de l’incuber et de mesurer l’extinction des gènes. La simplicité et la vitesse combinées à une efficacité exceptionnelle de transfection rendent le réactif de transfection Lipofectamine® RNAiMAX idéal pour les transfections d’ARNic à haut débit. Les conditions de transfection peuvent facilement être établies pour les systèmes robotique ou automatisés utilisés dans de telles applications.
Trouvez un protocole de transfection optimisé Lipofectamine® RNAiMAX pour votre lignée cellulaire.
Oligofectamine™ Transfection Reagent (Invitrogen™)
Le réactif de transfection Oligofectamine™ est une formulation exclusive pour la transfection des oligonucléotides et de l’ARN interférent court (ARNic) en cellules eucaryotes. Le réactif de transfection Oligofectamine™ forme des complexes stables avec les oligos, permettant une transfection efficace en cellules eucaryotes de façon hautement spécifique et cependant non toxique. Le réactif Oligofectamine™ convient aux cibles nucléaires et cytoplasmiques et transfecte une grande variété de lignées cellulaires dont CHO, HEK-293, NIH 3T3 et HeLa.
Utilisation du réactif de transfection Oligofectamine™
Le réactif Oligofectamine™ est facile à utiliser car il offre un protocole simple et rapide. Il vous suffit de diluer le réactif Oligofectamine™, de le mélanger avec les oligonucléotides et de l’ajouter à vos cellules. Le réactif Oligofectamine™ nécessite des quantités nanomolaires d’oligonucléotide antisens, réduisant la quantité du précieux oligonucléotide nécessaire jusqu’à un millier de fois. Ce qui en fait un réactif idéal pour les applications à haut débit. En outre, le réactif Oligofectamine™ s’est aussi avéré capable de fonctionner pour les transfections de ARNic. Nous recommandons ce réactif pour l’exécution d’expériences d’extinction de gènes utilisant les ARNic dans les cellules HeLa. Pour de plus amples informations, veuillez consulter RANi Central
Utilisation du réactif de transfection Oligofectamine™
Le réactif Oligofectamine™ est facile à utiliser car il offre un protocole simple et rapide. Il vous suffit de diluer le réactif Oligofectamine™, de le mélanger avec les oligonucléotides et de l’ajouter à vos cellules. Le réactif Oligofectamine™ nécessite des quantités nanomolaires d’oligonucléotide antisens, réduisant la quantité du précieux oligonucléotide nécessaire jusqu’à un millier de fois. Ce qui en fait un réactif idéal pour les applications à haut débit. En outre, le réactif Oligofectamine™ s’est aussi avéré capable de fonctionner pour les transfections de ARNic. Nous recommandons ce réactif pour l’exécution d’expériences d’extinction de gènes utilisant les ARNic dans les cellules HeLa. Pour de plus amples informations, veuillez consulter RANi Central
ExpiFectamine™ 293 Transfection Kit (Gibco™)
Le kit de transfection ExpiFectamine™ 293 est un composant clé du système d’expression Expi293™. Il est conçu pour la transfection transitoire des cultures de cellules rénales embryonnaires humaines (HEK) 293 haute densité. Le réactif et les activateurs cationiques de transfection à base de lipides à haute efficacité sont conçus pour alimenter le niveau le plus élevé possible d’expression protéique des cellules Expi293F™ cultivées sur le milieu d’expression Expi293™
• Conçu spécifiquement pour la transfection de cultures cellulaires en suspension haute densité, avec des activateurs correspondants qui renforcent l’efficacité de la transfection et de l’expression protéique
• Permet d’obtenir des rendements protéiques de 2 à 10 fois supérieurs aux autres réactifs de transfection utilisés sur les cultures cellulaires 293 haute densité
• Emploie les mêmes protocoles d’expression transitoire que ceux utilisés dans les systèmes de culture en suspension 293 basse densité, afin de passer facilement d’un système basse densité au système d’expression Expi293™ haute densité à rendement élevé
• Assure des résultats de transfection solides et reproductibles, permettant de renforcer la confiance des utilisateurs dans leurs résultats
• Adapte les transfections pour les volumes de cultures inférieures à 1 ml jusqu’à ceux supérieurs à 10 litres, tout en maintenant des rendements protéiques volumétriques équivalents
Le kit de transfection ExpiFectamine™ 293 a été conçu spécifiquement pour fonctionner avec les cellules Expi293F™ du système d’expression Expi293™. Ce kit de transfection comprend le réactif cationique à base de lipides ExpiFectamine™ 293 et les activateurs de transfection ExpiFectamine™ 293 1 et 2. Les activateurs sont ajoutés 16 à 18 heures post-transfection afin de renforcer la transfection, la viabilité cellulaire et l’expression protéique. Le protocole d’utilisation des composants de transfection ExpiFectamine™ 293 est quasi identique aux protocoles de transfection pour les autres réactifs d’expression transitoires, comme le 293fectin et le FreeStyle™ MAX.
• Conçu spécifiquement pour la transfection de cultures cellulaires en suspension haute densité, avec des activateurs correspondants qui renforcent l’efficacité de la transfection et de l’expression protéique
• Permet d’obtenir des rendements protéiques de 2 à 10 fois supérieurs aux autres réactifs de transfection utilisés sur les cultures cellulaires 293 haute densité
• Emploie les mêmes protocoles d’expression transitoire que ceux utilisés dans les systèmes de culture en suspension 293 basse densité, afin de passer facilement d’un système basse densité au système d’expression Expi293™ haute densité à rendement élevé
• Assure des résultats de transfection solides et reproductibles, permettant de renforcer la confiance des utilisateurs dans leurs résultats
• Adapte les transfections pour les volumes de cultures inférieures à 1 ml jusqu’à ceux supérieurs à 10 litres, tout en maintenant des rendements protéiques volumétriques équivalents
Le kit de transfection ExpiFectamine™ 293 a été conçu spécifiquement pour fonctionner avec les cellules Expi293F™ du système d’expression Expi293™. Ce kit de transfection comprend le réactif cationique à base de lipides ExpiFectamine™ 293 et les activateurs de transfection ExpiFectamine™ 293 1 et 2. Les activateurs sont ajoutés 16 à 18 heures post-transfection afin de renforcer la transfection, la viabilité cellulaire et l’expression protéique. Le protocole d’utilisation des composants de transfection ExpiFectamine™ 293 est quasi identique aux protocoles de transfection pour les autres réactifs d’expression transitoires, comme le 293fectin et le FreeStyle™ MAX.
Lipofectamine™ Stem Transfection Reagent (Invitrogen™)
Lipofectamine Stem Transfection Reagent is optimized to achieve maximum efficiency with minimal early differentiation in a wide range of stem cells. It can co-deliver DNA, RNA, and Cas9 ribonucleoprotein (RNP) complexes. Lipofectamine Stem reagent is compatible with a variety of media systems, including feeder-free, helping support and simplify your stem cell culture workflow. Researchers can achieve up to 80% or better transfection efficiency in pluripotent stem cells (PSCs) and neural stem cells (NSCs), and up to 45% in meschenchymal stem cells (MSCs).
Transfecting stem cells without inhibiting cell viability and cell growth can be challenging due to the sensitivity of these cells. Lipofectamine Stem is a low toxicity reagent, requiring low amounts of nucleic acid, allowing your stem cells to stay healthy and continue proliferating without inducing differentiation. Lipofectamine Stem reagent is:
Superior in performance—up to 3-fold higher efficiency across iPSCs, hESCs, NSCs, and MSCs compared to other reagents
Versatile—one reagent for DNA, RNA, Cas9 protein, and co-transfection
Gentle on cells—maintains cell proliferation without inducing differentiation
Flexible—transfects cells in adherence or suspension—an alternative to electroporation
Achieve high efficiency gene editing in stem cells
Gene editing applications typically require the ability to co-deliver DNA, RNA, and/or protein into cells. Lipofectamine Stem Transfection Reagent is designed to co-deliver CRISPR/Cas9 complexes into human stem cells for high efficiency gene editing. This one reagent can be used to transfect large DNA plasmids such as all-in-one CRISPR/Cas9 vectors and co-deliver a range of RNAs, such as large Cas9 mRNA with tracr/crRNA complexes or single guide (sg) RNA, and protein such as Cas9 protein combined with tracr/crRNA or sgRNA.
A low-toxicity alternative to electroporation
Transfecting stem cells via electroporation methods can be challenging and cumbersome. Electroporation protocols recommend single cell suspension culture to ensure maximum efficiency and minimal toxicity. Using the reverse transfection protocol, the Lipofectamine Stem Transfection Reagent proves to be an effective and less toxic alternative to electroporation for stem cells. It requires low amounts of nucleic acid to achieve maximum transfection efficiency, on par with electroporation, while supporting continued proliferation without inducing differentiation.
Transfecting stem cells without inhibiting cell viability and cell growth can be challenging due to the sensitivity of these cells. Lipofectamine Stem is a low toxicity reagent, requiring low amounts of nucleic acid, allowing your stem cells to stay healthy and continue proliferating without inducing differentiation. Lipofectamine Stem reagent is:
Superior in performance—up to 3-fold higher efficiency across iPSCs, hESCs, NSCs, and MSCs compared to other reagents
Versatile—one reagent for DNA, RNA, Cas9 protein, and co-transfection
Gentle on cells—maintains cell proliferation without inducing differentiation
Flexible—transfects cells in adherence or suspension—an alternative to electroporation
Achieve high efficiency gene editing in stem cells
Gene editing applications typically require the ability to co-deliver DNA, RNA, and/or protein into cells. Lipofectamine Stem Transfection Reagent is designed to co-deliver CRISPR/Cas9 complexes into human stem cells for high efficiency gene editing. This one reagent can be used to transfect large DNA plasmids such as all-in-one CRISPR/Cas9 vectors and co-deliver a range of RNAs, such as large Cas9 mRNA with tracr/crRNA complexes or single guide (sg) RNA, and protein such as Cas9 protein combined with tracr/crRNA or sgRNA.
A low-toxicity alternative to electroporation
Transfecting stem cells via electroporation methods can be challenging and cumbersome. Electroporation protocols recommend single cell suspension culture to ensure maximum efficiency and minimal toxicity. Using the reverse transfection protocol, the Lipofectamine Stem Transfection Reagent proves to be an effective and less toxic alternative to electroporation for stem cells. It requires low amounts of nucleic acid to achieve maximum transfection efficiency, on par with electroporation, while supporting continued proliferation without inducing differentiation.
Pierce™ Protein Transfection Reagent (Thermo Scientific™)
Thermo Scientific Pierce Protein Transfection Reagent (formerly Pro-Ject) is a cationic lipid mixture for complexation with proteins, peptides, antibodies and other biologically active molecules to allow their direct intracellular delivery.
Features of Protein Transfection Reagent:
• No need to isolate, clone and transfect gene sequences—Saves days of work
• Optimum delivery in three to four hours after incubation—Fast protein delivery
• Delivery complex is non-covalent—Proteins and peptides retain biological activity after delivery
• Works with proteins, peptides and fluorescently labeled antibodies—Versatility
This is a unique cationic lipid mixture (previously called Pro-Ject Reagent) which, when complexed with proteins or peptides, allows direct intracellular delivery of proteins, peptides, antibodies and other biologically active molecules. Reagent protein complexes attach to negatively charged cell surfaces and either can directly fuse with the membrane and deliver the captured protein into the cell or be endocytosed by the cell and then fuse with the endosome, releasing the captured protein into the cytoplasm. Because the complex formed is noncovalent it does not interfere with the protein's biological activity.
Molecules that have been successfully delivered with the transfection reagent include β-galactosidase, fluorescent-antibody, green fluorescent protein (GFP), high and low molecular weight dextran sulfate, phycoerythrin-BSA, caspase 3, caspase 8 and granzyme B.
Protocol for Optimization of Protein Transfection.
Features of Protein Transfection Reagent:
• No need to isolate, clone and transfect gene sequences—Saves days of work
• Optimum delivery in three to four hours after incubation—Fast protein delivery
• Delivery complex is non-covalent—Proteins and peptides retain biological activity after delivery
• Works with proteins, peptides and fluorescently labeled antibodies—Versatility
This is a unique cationic lipid mixture (previously called Pro-Ject Reagent) which, when complexed with proteins or peptides, allows direct intracellular delivery of proteins, peptides, antibodies and other biologically active molecules. Reagent protein complexes attach to negatively charged cell surfaces and either can directly fuse with the membrane and deliver the captured protein into the cell or be endocytosed by the cell and then fuse with the endosome, releasing the captured protein into the cytoplasm. Because the complex formed is noncovalent it does not interfere with the protein's biological activity.
Molecules that have been successfully delivered with the transfection reagent include β-galactosidase, fluorescent-antibody, green fluorescent protein (GFP), high and low molecular weight dextran sulfate, phycoerythrin-BSA, caspase 3, caspase 8 and granzyme B.
Protocol for Optimization of Protein Transfection.
siPORT™ Amine Transfection Agent with manual (Invitrogen™)
A polyamine-based Ambion® siRNA transfection agent that can be used to transfect cells during subculturing-—saving a full day of valuable time—without increasing toxicity. It is supplied in one tube containing 1 mL. A successful siRNA experiment requires the appropriate transfection agent for your cells, and one that is optimized for siRNA delivery. The efficiency with which different transfection reagents deliver nucleic acids can vary dramatically with the type of nucleic acid being transfected (RNA vs. DNA, single-stranded vs. double-stranded) and the cells being used.
Accessory Products:
siPORT™ NeoFX™ Transfection Agent (SKU#s AM4511 and AM4510), a cationic and neutral lipid mixture, is also available. For testing which transfection agent is best with your system, the Silencer® siRNA Transfection II Kit (SKU# AM1631) is recommended. It contains the two different transfection agents along with controls for transfection optimization.
Accessory Products:
siPORT™ NeoFX™ Transfection Agent (SKU#s AM4511 and AM4510), a cationic and neutral lipid mixture, is also available. For testing which transfection agent is best with your system, the Silencer® siRNA Transfection II Kit (SKU# AM1631) is recommended. It contains the two different transfection agents along with controls for transfection optimization.
Invivofectamine™ 3.0 Reagent (Invitrogen™)
Invivofectamine® 3.0 Reagent is an animal origin-free lipid nanoparticle designed for high efficiency in vivo delivery of siRNA and miRNA to mouse liver cells following tail vein injection.
Using Invivofectamine® 3.0 Reagent
Invivofectamine® 3.0 Reagent is ideally suited for in vivo delivery of siRNA and miRNA duplexes. Invivofectamine® 3.0/RNA complexes are delivered using a low-volume, low-pressure method with no toxicity and no stress response in the animal. Invivofectamine® 3.0 can also be combined with BLOCK-iT™ fluorescent controls to track the biodistribution of the siRNA or miRNA.
Using Invivofectamine® 3.0 Reagent
Invivofectamine® 3.0 Reagent is ideally suited for in vivo delivery of siRNA and miRNA duplexes. Invivofectamine® 3.0/RNA complexes are delivered using a low-volume, low-pressure method with no toxicity and no stress response in the animal. Invivofectamine® 3.0 can also be combined with BLOCK-iT™ fluorescent controls to track the biodistribution of the siRNA or miRNA.
siPORT™ NeoFX™ Transfection Agent with manual (Invitrogen™)
This Ambion® lipid-based formulation can be used to efficiently transfect adherent cells with siRNA as they are subcultured—without increased cytotoxicity. It is provided in one tube containing 1 mL.
• Versatile—works with a broad range of cell lines
• Reproducible—produces consistent results, lot-to-lot, plate-to-plate, and well-to-well
• Efficient—performs with high siRNA transfection efficiency, allowing use of low siRNA concentrations to minimize nonspecific effects
• Fast—reverse-transfects cells as they are plated, saving a day
Gene silencing experiments often call for critical, but time-consuming optimization experiments. siPORT™ NeoFX™ Transfection Agent refines siRNA transfection protocols resulting in less optimization. From start to finish, successful gene silencing experiments can be completed in as early as 24 hours. This streamlined protocol can be adapted to a wide range of cells and experimental designs, including high-throughput applications.
Reverse-Transfect Cells as They are Plated, Saving a Day
Just add siPORT™ NeoFX™ to your diluted siRNA, incubate to form transfection complexes, add the complexes to the culture wells, and overlay with cells. This new streamlined protocol, named "neofection", will save researchers a full day of valuable time when compared to traditional plated transfection procedures. The transfection complexes are active and stable even in the presence of serum, so there is no need to remove or replace media following transfection.
Optimized for Lower siRNA Concentrations and High Reproducibility
Recent reports indicate that siRNA concentrations of 100 nM or higher can lead to nonspecific changes in gene expression (off-target effects) in mammalian cultured cells. Reducing the amount of siRNA used for transfections to 1–20 nM minimizes these nonspecific effects, while still providing extensive silencing of the target gene. siPORT™ NeoFX™ Transfection Agent efficiently transfects low concentrations of siRNA. Furthermore, this agent delivers high performance among multiple wells for replicate samples and between different lots of the transfection agent.
Compatible with a Broad Range of Cell Lines
The agent has been used successfully to deliver siRNA into many cell types, including MCF-7 (human breast adenocarcinoma), HT-29 (human colorectal adenocarcinoma), HeLa-S3 (human cervix adenocarcinoma), HeLa (human cervix adenocarcinoma), HepG2 (human hepatocellular carcinoma), BJ (human foreskin fibroblast), A549 (human lung carcinoma), UMR106 (rat osteosarcoma), SKOv3 (human ovarian carcinoma), and SKNAS (human bone marrow neuroblastoma).
Accessory Products:
siPORT™ Amine Transfection Agent (SKU#s AM4502 and AM4503), a polyamine agent, is also available. For testing which transfection agent is best with your system, the Silencer® siRNA Transfection II Kit (SKU# AM1631) is recommended. It contains the two different transfection agents along with controls for transfection optimization.
• Versatile—works with a broad range of cell lines
• Reproducible—produces consistent results, lot-to-lot, plate-to-plate, and well-to-well
• Efficient—performs with high siRNA transfection efficiency, allowing use of low siRNA concentrations to minimize nonspecific effects
• Fast—reverse-transfects cells as they are plated, saving a day
Gene silencing experiments often call for critical, but time-consuming optimization experiments. siPORT™ NeoFX™ Transfection Agent refines siRNA transfection protocols resulting in less optimization. From start to finish, successful gene silencing experiments can be completed in as early as 24 hours. This streamlined protocol can be adapted to a wide range of cells and experimental designs, including high-throughput applications.
Reverse-Transfect Cells as They are Plated, Saving a Day
Just add siPORT™ NeoFX™ to your diluted siRNA, incubate to form transfection complexes, add the complexes to the culture wells, and overlay with cells. This new streamlined protocol, named "neofection", will save researchers a full day of valuable time when compared to traditional plated transfection procedures. The transfection complexes are active and stable even in the presence of serum, so there is no need to remove or replace media following transfection.
Optimized for Lower siRNA Concentrations and High Reproducibility
Recent reports indicate that siRNA concentrations of 100 nM or higher can lead to nonspecific changes in gene expression (off-target effects) in mammalian cultured cells. Reducing the amount of siRNA used for transfections to 1–20 nM minimizes these nonspecific effects, while still providing extensive silencing of the target gene. siPORT™ NeoFX™ Transfection Agent efficiently transfects low concentrations of siRNA. Furthermore, this agent delivers high performance among multiple wells for replicate samples and between different lots of the transfection agent.
Compatible with a Broad Range of Cell Lines
The agent has been used successfully to deliver siRNA into many cell types, including MCF-7 (human breast adenocarcinoma), HT-29 (human colorectal adenocarcinoma), HeLa-S3 (human cervix adenocarcinoma), HeLa (human cervix adenocarcinoma), HepG2 (human hepatocellular carcinoma), BJ (human foreskin fibroblast), A549 (human lung carcinoma), UMR106 (rat osteosarcoma), SKOv3 (human ovarian carcinoma), and SKNAS (human bone marrow neuroblastoma).
Accessory Products:
siPORT™ Amine Transfection Agent (SKU#s AM4502 and AM4503), a polyamine agent, is also available. For testing which transfection agent is best with your system, the Silencer® siRNA Transfection II Kit (SKU# AM1631) is recommended. It contains the two different transfection agents along with controls for transfection optimization.
ExpiFectamine™ CHO Transfection Kit (Gibco™)
The ExpiFectamine™ CHO Transfection Kit is a core component of the ExpiCHO™ Expression System Kit (Cat. No. A29133). It is designed for transient transfection of high-density cultures of Chinese hamster ovary (CHO) cells. The high-efficiency, cationic transfection reagent, coupled with an optimized transfection enhancer and culture feed, will power the highest possible level of protein expression from ExpiCHO-S™ Cells (Cat. No. A29127) cultured in ExpiCHO™ Expression Medium (Cat. No. A2910001).
The ExpiFectamine CHO Transfection Kit:
• Achieves greater than 90% transfection efficiency of high-density suspension CHO cell culture, with matching transfection enhancer and culture feed to boost transfection performance and protein expression
• Requires one-third to one-half less plasmid DNA than other transfection reagents while achieving superior expression performance
• Provides options for three transient expression protocols for maximal work flexibility while still fitting into the same overall workflow used by other transient expression systems, such as FreeStyle™ MAX CHO and Expi293™
• Enables robust and reproducible transfection results, giving you greater confidence in your results
• Demonstrates linear scalability of transfections from culture volumes of <1 mL to >10 L
The ExpiFectamine CHO Transfection Kit is specifically designed to work with ExpiCHO-S Cells in the ExpiCHO™ Expression System Kit. The transfection kit supplies the cationic ExpiFectamine™ CHO Transfection Reagent, ExpiFectamine™ CHO Transfection Enhancer, and ExpiCHO Feed.
The ExpiFectamine CHO Transfection Kit:
• Achieves greater than 90% transfection efficiency of high-density suspension CHO cell culture, with matching transfection enhancer and culture feed to boost transfection performance and protein expression
• Requires one-third to one-half less plasmid DNA than other transfection reagents while achieving superior expression performance
• Provides options for three transient expression protocols for maximal work flexibility while still fitting into the same overall workflow used by other transient expression systems, such as FreeStyle™ MAX CHO and Expi293™
• Enables robust and reproducible transfection results, giving you greater confidence in your results
• Demonstrates linear scalability of transfections from culture volumes of <1 mL to >10 L
The ExpiFectamine CHO Transfection Kit is specifically designed to work with ExpiCHO-S Cells in the ExpiCHO™ Expression System Kit. The transfection kit supplies the cationic ExpiFectamine™ CHO Transfection Reagent, ExpiFectamine™ CHO Transfection Enhancer, and ExpiCHO Feed.
LV-MAX™ Lentiviral Production System (Gibco™)
The Gibco LV-MAX Lentiviral Production System is the first optimized system that provides a scalable and high-yield lentiviral vector production platform. It is based on a suspension, high-density culture of HEK 293-derived Viral Production Cells adapted to a special chemically defined, serum-free and protein-free LV-MAX Production Medium. Optimal viral titer yield is mediated by our advanced lipid nanoparticle transfection reagent in combination with a novel lentiviral-specific enhancer and supplement. All components work synergistically to generate superior and functional lentiviral particles under serum-free conditions compared to conventional polyethylenimine (PEI).
The LV-MAX Lentiviral Production System provides:
• High titer—greater than 1 x 108 TU/mL (unconcentrated LVVs-GFP)
• Scalable suspension system—from 96 deep-well plate to 2-L bioreactor
• Serum-free—eliminating animal-origin components and associated risks
• Easy, robust culture and transfection protocols—fits into current transient production workflows
The components included in the LV-MAX Lentiviral Production System are: two vials of frozen Viral Production Cells, one liter of LV-MAX Production Medium, and one LV-MAX Transfection Kit sufficient to produce 0.3 L of lentivirus. All components of the LV-MAX Lentiviral Production System are also available for purchase separately. Also required, but not included in this kit, is Gibco Opti-MEM I Reduced Serum Medium (Cat. No. 31985062).
The LV-MAX Lentiviral Production System provides:
• High titer—greater than 1 x 108 TU/mL (unconcentrated LVVs-GFP)
• Scalable suspension system—from 96 deep-well plate to 2-L bioreactor
• Serum-free—eliminating animal-origin components and associated risks
• Easy, robust culture and transfection protocols—fits into current transient production workflows
The components included in the LV-MAX Lentiviral Production System are: two vials of frozen Viral Production Cells, one liter of LV-MAX Production Medium, and one LV-MAX Transfection Kit sufficient to produce 0.3 L of lentivirus. All components of the LV-MAX Lentiviral Production System are also available for purchase separately. Also required, but not included in this kit, is Gibco Opti-MEM I Reduced Serum Medium (Cat. No. 31985062).
Lipofectamine™ 3000 Transfection Reagent (Invitrogen™)
Lipofectamine® 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results. This reagent delivers superior transfection efficiency and improved cell viability for the widest range of hard-to-transfect and common cells (e.g., HEK293, HeLa).
Successfully transfect the widest variety of biologically relevant cell types with a reagent that offers:
• Superior performance—our highest efficiency reagent for difficult-to-transfect cells
• Improved cell viability—gentle on your cells, with low toxicity
• Versatile—one reagent for DNA, RNA, and co-transfection
Efficiently Transfect Difficult-to-Transfect Cells
Lipofectamine® 3000 reagent is designed to efficiently transfect difficult-to-transfect cells, yielding superior transfection performance across the broadest array of cell types. Successfully transfected cell types include:
View performance data for 60+ cell lines plus comparison to Lipofectamine 2000 >
Transfect Common Cells Efficiently and Cost-Effectively
For common cell types, Lipofectamine® 3000 reagent is more cost-effective than other reagents due to its high concentration and low usage amount. The 1.5 mL size provides up to 1500 transfection reactions (in 24-well plates).
Achieve Superior Transfection Efficiency While Maintaining Low Toxicity
Lipofectamine® 3000 reagent maintains a high transfection efficiency within a robust dynamic range of lipid doses for quick and easy optimization. A low toxicity lipid dose (0.75 µL for 24-well plates) is suggested for applications requiring minimal disruption of the cells.
Enable High Transfection Efficiency in Novel Genome Editing Applications
Lipofectamine® 3000 reagent is suitable for novel genome editing applications. It increases the likelihood of successful cleavage and recombination with TALENs or CRISPRs through highly efficient transfection, ultimately maximizing the efficiency of genetic modifications and simplifying the downstream processes.
Successfully transfect the widest variety of biologically relevant cell types with a reagent that offers:
• Superior performance—our highest efficiency reagent for difficult-to-transfect cells
• Improved cell viability—gentle on your cells, with low toxicity
• Versatile—one reagent for DNA, RNA, and co-transfection
Efficiently Transfect Difficult-to-Transfect Cells
Lipofectamine® 3000 reagent is designed to efficiently transfect difficult-to-transfect cells, yielding superior transfection performance across the broadest array of cell types. Successfully transfected cell types include:
| Fibroblast (3T3, COS-7) | Myoblast (C2C12, L6 CRL-1458) |
| Hepatocyte (HepG2, HuH7) | Erythroleukemic cell (K562) |
| Breast carcinoma (MCF7, Hs578T) | Prostate carcinoma (LNCap) |
| Lung carcinoma (A549, NCI-H460) | Osteosarcoma (U-2 OS, Saos-2) |
| Colon carcinoma (Caco2, SW480) | Pancreatic carcinoma (PANC-1) |
| Skin melanoma (SK-MEL-28) |
View performance data for 60+ cell lines plus comparison to Lipofectamine 2000 >
Transfect Common Cells Efficiently and Cost-Effectively
For common cell types, Lipofectamine® 3000 reagent is more cost-effective than other reagents due to its high concentration and low usage amount. The 1.5 mL size provides up to 1500 transfection reactions (in 24-well plates).
Achieve Superior Transfection Efficiency While Maintaining Low Toxicity
Lipofectamine® 3000 reagent maintains a high transfection efficiency within a robust dynamic range of lipid doses for quick and easy optimization. A low toxicity lipid dose (0.75 µL for 24-well plates) is suggested for applications requiring minimal disruption of the cells.
Enable High Transfection Efficiency in Novel Genome Editing Applications
Lipofectamine® 3000 reagent is suitable for novel genome editing applications. It increases the likelihood of successful cleavage and recombination with TALENs or CRISPRs through highly efficient transfection, ultimately maximizing the efficiency of genetic modifications and simplifying the downstream processes.
PLUS™ Reagent (Invitrogen™)
Le réactif PLUS™ est utilisé conjointement à divers réactifs de transfection tels que le réactif Lipofectamine™ dans le but d’améliorer l’efficacité de la transfection au sein des lignées de cellules adhérentes.
Utilisation du réactif PLUS™
Certains clients trouvent que l’utilisation du réactif PLUS™ permet de limiter l’optimisation de la concentration d’ADN nécessaire à l’obtention de bonnes performances en matière de transfection. L’ajout de réactif PLUS™ pourrait également réduire de moitié environ les quantités d’ADN et de lipides requises par rapport à l’utilisation de lipides seuls. Le réactif PLUS™ a été spécifiquement conçu pour élargir le nombre de types de cellules transfectables. Associé au réactif Lipofectamine™, le réactif PLUS™ a prouvé ses capacités d’amélioration de la transfection de BHK-21, NIH 3T3 et autres lignées cellulaires.
Utilisation du réactif PLUS™
Certains clients trouvent que l’utilisation du réactif PLUS™ permet de limiter l’optimisation de la concentration d’ADN nécessaire à l’obtention de bonnes performances en matière de transfection. L’ajout de réactif PLUS™ pourrait également réduire de moitié environ les quantités d’ADN et de lipides requises par rapport à l’utilisation de lipides seuls. Le réactif PLUS™ a été spécifiquement conçu pour élargir le nombre de types de cellules transfectables. Associé au réactif Lipofectamine™, le réactif PLUS™ a prouvé ses capacités d’amélioration de la transfection de BHK-21, NIH 3T3 et autres lignées cellulaires.
PEI-Transferrinfection Kit (Invitrogen™)
Transfection of DNA into eukaryotic cells is a common method to study biological mechanisms. A major goal is the efficient and specific delivery of genes into the desired target cells. Although a wide panel of techniques and vectors (viral and nonviral) have been developed that work with variable efficiency, most vectors lack a target cell specificity. Nonviral vectors are attractive because of their ease of manipulation, safety and high flexibility in the size of the delivered transgene. The Transferrinfection is a high-efficiency nucleic acid delivery system based on transferrin receptor-mediated endocytosis to carry DNA into cells. Furthermore it was shown that the cationic polymer polyethylenimine (PEI) mediates efficient gene transfer into a variety of cells. In the Polyethylenimine-Transferrinfection system the gene transfer efficiency of PEI/DNA complexes are combined with the specific mechanism of receptor-mediated endocytosis via Transferrin receptor.
The method results in a 30 - 1000-fold enhanced transfection efficiency depending on the cell line. It is an extremely gentle DNA transfection method whichemploys physiological uptake mechanisms of the cell. Transfection efficacy depends on the cell type, the level of surface transferrin receptor expression. Very high transfection rates have been shown for the tested tumor cell lines B16F10 melanoma, Neuro 2A neuroblastoma and a variety of primary human melanoma cell lines. In other established cell lines, such as HeLa, CHO, Jurkat, K562, HepG2 and COS the PEI-Transferrinfection works with high efficiences, excellent reproducibility and with the advantage of being an extremely gentle procedure.
Reactivity/Species
Human
Suitable Sample Types
Suspension cultures, Adherent cells
Reported Application
Cell Culture
The method results in a 30 - 1000-fold enhanced transfection efficiency depending on the cell line. It is an extremely gentle DNA transfection method whichemploys physiological uptake mechanisms of the cell. Transfection efficacy depends on the cell type, the level of surface transferrin receptor expression. Very high transfection rates have been shown for the tested tumor cell lines B16F10 melanoma, Neuro 2A neuroblastoma and a variety of primary human melanoma cell lines. In other established cell lines, such as HeLa, CHO, Jurkat, K562, HepG2 and COS the PEI-Transferrinfection works with high efficiences, excellent reproducibility and with the advantage of being an extremely gentle procedure.
Reactivity/Species
Human
Suitable Sample Types
Suspension cultures, Adherent cells
Reported Application
Cell Culture
FreeStyle™ MAX Reagent (Gibco™)
FreeStyle™ MAX Reagent is a proprietary, animal origin–free formulation for transfecting plasmid DNA into eukaryotic cells that can easily be scaled up to produce large amounts of recombinant proteins. FreeStyle™ MAX Reagent allows the highest expression levels and transfection rates with lowest cytotoxicity in bio-production applications, and is specifically formulated for use with:
• FreeStyle™ 293-F Cells (suspension human embryonal kidney cells, Cat. No. R790-07) in serum-free FreeStyle™ 293 Expression Medium (Cat. No. 12338-018)
• FreeStyle™ CHO-S Cells (suspension Chinese Hamster Ovary cells, Cat. No. R800-07) in serum-free FreeStyle™ CHO Expression Medium (Cat. No. 12651-014)
• DG44 Cells (DHFR– suspension CHO cells) in CD DG44 Medium with 8 mM L-glutamine and 18 mL/L 10% Pluronic® F-68 (Cat. No. A11000-01)
Product use
FreeStyle™ MAX Reagent is intended for use with the FreeStyle™ MAX 293 Expression System (Cat. No. K9000-10) and the FreeStyle™ MAX CHO Expression System (Cat. No. K9000-20).
• FreeStyle™ 293-F Cells (suspension human embryonal kidney cells, Cat. No. R790-07) in serum-free FreeStyle™ 293 Expression Medium (Cat. No. 12338-018)
• FreeStyle™ CHO-S Cells (suspension Chinese Hamster Ovary cells, Cat. No. R800-07) in serum-free FreeStyle™ CHO Expression Medium (Cat. No. 12651-014)
• DG44 Cells (DHFR– suspension CHO cells) in CD DG44 Medium with 8 mM L-glutamine and 18 mL/L 10% Pluronic® F-68 (Cat. No. A11000-01)
Product use
FreeStyle™ MAX Reagent is intended for use with the FreeStyle™ MAX 293 Expression System (Cat. No. K9000-10) and the FreeStyle™ MAX CHO Expression System (Cat. No. K9000-20).
