Shop All LC Reversed-Phase Columns, Media, & Standards

POROS™ R2 10 µm Column, 2.1 x 30 mm, 0.1 mL Thermo Scientific™

Applied Biosystems™ POROS™ Prepacked Reversed-Phase Column. POROS™ R2 is a 2000 angstrom pore size Poly[styrenevinylbenzene] particle, suitable for the reversed-phase separation of biomolecules.
10 micron particle size is used for the highest resolution chromatography, and is suitable for analytical to small scale preparative separation of biomolecules.

POROS™ High Performance Chromatography™ Media and Pre-Packed Columns

A high performance chromatography resins for analytical to process scale separations.• Higher productivity: High throughput and high dynamic capacity associated with High Performance Chromatography™
• Chemical stability: Allows aggressive cleaning and sanitization
• Enhanced biomolecule access: Provided via large pores, ranging between 500-10000 Å
• Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
• Develop better separations methods in a shorter time frame: The speed of High Performance Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep

You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis

High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High capacity, high resolution, high speed
In contrast to conventional chromatography media, POROS™ High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large "throughpores" allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short "diffusive" pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm⁄hr compared to 50 to 360 cm⁄hr for conventional media.

Hypersil GOLD™ C8 HPLC Columns Thermo Scientific™

For analytes with medium hydrophobicity or when a less hydrophobic phase is required to obtain optimum retention, use Thermo Scientific™ Hypersil GOLD™ C8 LC columns for outstanding sensitivity and excellent peak shape. The selectivity is similar to C18 columns with a predictable elution order but less retention. The lower hydrophobicity allows compounds to elute more quickly, yielding faster separations.

Hypersil™ BDS C18 Columns Thermo Scientific™

For a robust, general-purpose column to use in a wide range of applications, choose Thermo Scientific™ Hypersil™ BDS C18 HPLC Columns. These economical columns feature reduced silanol activity and provide exceptional reliability for QA/QC labs. Built on the renowned Hypersil silica backbone, the base-deactivated silica is one of the most popular packing materials available.

For more challenging separations that require improved chromatographic performance, use Thermo Scientific™ Hypersil GOLD™ columns as an alternative.

Accucore™ 150 C18 HPLC Columns Thermo Scientific™

Offer phase characteristics designed for the separation of peptides with Thermo Scientific™ Accucore™ 150 C18 LC Columns. Based on Core Enhanced Technology, Accucore columns provide fast, high-resolution biomolecule separations without the elevated backpressures required by sub-2μm particles.

Hypersil GOLD™ C18 Selectivity HPLC Columns Thermo Scientific™

Achieve exceptional peak shape and resolution for your HPLC and LC/MS applications with Thermo Scientific™ Hypersil GOLD™ HPLC columns. These endcapped, ultrapure, silica-based columns deliver significant reduction in peak tailing using generic gradients with C18 selectivity. With their excellent resolution, efficiency, and sensitivity, Hypersil GOLD columns give you confidence in the accuracy and quality of your analytical data.

Hypersil™ BDS C8 HPLC Columns Thermo Scientific™

For a high-quality, base-deactivated, fully endcapped phase with similar selectivity to C18 but slightly less retention, use Thermo Scientific™ Hypersil™ BDS C8 HPLC Columns. These economical columns feature reduced silanol activity and provide exceptional reliability for QA/QC labs. Built on the renowned Hypersil silica backbone, the base-deactivated silica is one of the most popular packing materials available.

For more challenging separations that require improved chromatographic performance, use Thermo Scientific™ Hypersil GOLD™ C8 columns as an alternative.

Acclaim™ Phenyl-1 HPLC Columns Thermo Scientific™

For unique selectivity of aromatic compounds and superior chromatographic performance, choose Thermo Scientific™ Acclaim™ Phenyl-1 reversed-phase LC columns. The specially designed bonded phase displays greater π-π interaction than other phenyl phases and provides enhanced aromatic selectivity and higher hydrophobic retention. These columns are ideally suited for the analysis of aromatic analytes, including glucocorticosteroids, estrogens, fat-soluble vitamins, and phospholipids.

Accucore™ XL C8 HPLC Columns Thermo Scientific™

Optimize conventional HPLC methods with Thermo Scientific™ Accucore™ XL C8 columns. Rugged 4µm solid-core particles ensure very high efficiencies within standard HPLC pressure limits. The high coverage C8 bonded phase provides optimal retention of analytes with moderate hydrophobicity. Robust bonding technology and automated packing procedures ensure excellent reproducibility and long column lifetimes.

POROS™ R2 10 µm Column, 2.1 x 100 mm, 0.3 mL Thermo Scientific™

Applied Biosystems™ POROS™ Prepacked Reversed-Phase Column. POROS™ R2 is a 2000 angstrom pore size Poly[styrenevinylbenzene] particle, suitable for the reversed-phase separation of biomolecules.
10 micron particle size is used for the highest resolution chromatography, and is suitable for analytical to small scale preparative separation of biomolecules.

POROS™ High Performance Chromatography™ Media and Pre-Packed Columns

A high performance chromatography resins for analytical to process scale separations.• Higher productivity: High throughput and high dynamic capacity associated with High Performance Chromatography™
• Chemical stability: Allows aggressive cleaning and sanitization
• Enhanced biomolecule access: Provided via large pores, ranging between 500-10000 Å
• Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
• Develop better separations methods in a shorter time frame: The speed of High Performance Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep

You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis

High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High capacity, high resolution, high speed
In contrast to conventional chromatography media, POROS™ High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large "throughpores" allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short "diffusive" pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm⁄hr compared to 50 to 360 cm⁄hr for conventional media.

Hypersil™ Phenyl HPLC Columns Thermo Scientific™

Effectively separate aromatic compounds and moderately polar compounds with Thermo Scientific™ Hypersil™ Phenyl LC Columns.

 

Classical Hypersil stationary phases are recognized as an industry standard in HPLC. Proven as an effective analytical tool, classical Hypersil phases are well established and referenced in HPLC methods. Quality is assured by manufacture under ISO 9001-2000 quality guidelines.

For more challenging separations that require improved chromatographic performance, use Thermo Scientific™ Hypersil GOLD™ Phenyl columns as an alternative.

Accucore™ C18 HPLC Columns Thermo Scientific™

Achieve fast, high-resolution separations at low backpressures using Thermo Scientific™ Accucore™ C18 LC columns. Rugged 2.6µm solid-core particles ensure high efficiencies and enable compatibility with both HPLC and UHPLC platforms. The high bonded phase coverage provides optimal retention of a broad range of nonpolar analytes across multiple applications. Robust bonding technology and automated packing procedures ensure excellent reproducibility and long column lifetimes.

Accucore™ XL C18 HPLC Columns Thermo Scientific™

Optimize conventional HPLC methods with Thermo Scientific™ Accucore™ XL C18 LC Columns. Rugged 4µm solid-core particles ensure very high efficiencies within standard HPLC pressure limits. The high bonded phase coverage provides optimal retention of a broad range of nonpolar analytes across multiple applications. Robust bonding technology and automated packing procedures ensure excellent reproducibility and long column lifetimes.

EASY-Spray™ HPLC Columns Thermo Scientific™

Ensure robust nano and capillary flow LC-MS with Thermo Scientific™ EASY-Spray™ LC Columns. The integrated column/emitter design eliminates dead volume and is temperature-controlled for maximum reliability and performance. Rigourously tested to ensure maximum quality, these columns are designed for maximum simplicity and ease-of-use.

PepMap™ 100 C8 HPLC Columns Thermo Scientific™

Choose Thermo Scientific™ Acclaim™ PepMap™ 100 C8 LC Columns when separating very hydrophobic peptides (nontryptic peptides) as an alternative to the PepMap™ C18 column.

Hypersil GOLD™ C4 HPLC Columns Thermo Scientific™

For very hydrophobic analytes, achieve faster separations with excellent peak shapes using Thermo Scientific™ Hypersil GOLD™ C4 HPLC columns. Featuring a lower hydrophobicity than C18 or C8 columns, Hypersil GOLD C4 columns provide outstanding sensitivity and high efficiency.
Results per page
    spinner