Shop All Additional Selective and Differential Culture Media

Formulation


Solution Type


State at Room Temperature


Without Additives

Macrophage-SFM (1X) Gibco™

Macrophage-SFM is a serum-free medium designed for the culture of human peripheral blood monocytes and macrophages (1).

Contains L-glutamine, but no antibiotics.

AmnioMAX™-II Complete Medium Gibco™

Gibco® AmnioMAX™ - II Complete Medium is a fully-supplemented medium developed for the short term culture of human amniotic fluid cells for cytogenetic studies and in vitro diagnostic procedures.

Gibco® AmnioMAX™ - II Complete Medium features:

• Ready-to-use format
• Quality and performance testing
• Unique, optimized formulation

Ready-to-use format
Gibco® AmnioMAX™ - II Complete Medium is a frozen, 1X medium, ready to use upon thawing. No supplementation is required. Thawed medium can be stored at 2–8°C for up to ten days.

Quality and performance testing
Every lot of Gibco® AmnioMAX™ - II Complete Medium is performance tested by a certified US reference cytogenetics laboratory to ensure consistently superior performance. Pooled primary human amniotic fluid samples are cultured for six days in Gibco® AmnioMAX™ - II Complete Medium before measuring the total number of colonies and total number of mitotic colonies. In addition, each lot is tested for sterility, pH and osmolality.

Unique, optimized formulation
Gibco® AmnioMAX™ - II Complete Medium contains Fetal Bovine Serum (FBS), gentamicin, and L-glutamine to maximize cell attachment and growth. This optimized medium also has an enhanced buffering system that provides greater pH stability during culture manipulations.

Product Use
For in vitro diagnostic use.

cGMP Manufacturing and Quality System
Gibco® AmnioMAX™ - II Complete Medium is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Keratinocyte SFM (1X), without calcium chloride Gibco™

Keratinocyte SFM (serum-free medium) is optimized for the growth and maintenance of human keratinocytes and other types of epithelial cells without the need for a feeder layer of cells. The complete medium may be used to cultivate human epidermal keratinocytes for studies involving dermal substitutes, in vitro toxicology, and gene therapy. Keratinocyte SFM without calcium is available to allow researchers to adjust the calcium concentration to meet the demands of their specific application.

Keratinocyte SFM is a complete serum-free medium when supplemented with human recombinant epidermal growth factor (rEGF) and bovine pituitary extract (BPE) at the time of use. Both supplements, rEGF and BPE, are supplied in separate packaging with the basal medium.

EfficientFeed™ B+ AGT™ Supplement Gibco™

Gibco® EfficientFeed™ B+ (Plus) AGT™ Supplement is a dry-format, single-part supplement for fed batch addition to the culture of multiple cell lines to assist with process development and maximization of bioreactor utilization. Compared to original CHO CD EfficientFeed™ B AGT™ nutrient supplement, EfficientFeed™ B+ supplement has components that are uniquely combined to allow simpler single-part pH-neutral reconstitution, as well as the ability to super-concentrate the supplement from 52.7 g/L (1X) to 158.1g/L (3X) for reduction of product dilution or increased titers with additional feeding, when appropriate. The product is an animal origin-free, chemically defined formulation that contains no proteins, hydrolysates, or components of incompletely defined composition.

Which EfficientFeed™ + Supplement to Choose?

Consider EfficientFeed™ B+ AGT™ Supplement when:
• You have used CHO CD EfficientFeed™ B supplement in the past and wish to explore simpler reconstitution and super-concentration benefits of new technology for delivery of key components
• You are currently using CD CHO as your base cell growth medium

Consider EfficientFeed™ A+ AGT™ Supplement when:
• You have used CHO CD EfficientFeed™ A supplement in the past and wish to explore simpler reconstitution and super-concentration benefits of new technology for delivery of key components
• You are currently using CD OptiCHO™ as your base cell growth medium

Consider EfficientFeed™ C+ AGT™ Supplement when:
• You have used CD EfficientFeed™ C supplement in the past and wish to explore simpler reconstitution and super-concentration benefits of new technology for delivery of key components
• You want higher productivity in GS CHO, CHO-K1, & CHO-S® derived cell lines
• You are currently using CD FortiCHO™ as your base cell growth medium
• You are using a base medium with greater than 6 g/L glucose or a hydrolysate at day 0

Increasing Titers
As a complete feed, EfficientFeed™ + supplements have been shown to provide multiple-fold increases in titers at 1X concentration versus batch culture, and are an excellent starting point for process development. Flexibility to further increase titers are enhanced with the ability to super-concentrate the product by reconstituting with less water to deliver up to 158 g/L of fed nutrients (see figure).

Maximizing Bioreactor Utilization
Have you ever wished you could have more working volume at the start of a bioreactor run to get more protein produced, but needed too much feeding volume space to accomplish your optimal goal? Have you ever thought that room for a little more feeding might get you to a new level of titer from a process and maybe even reduce the number of bioreactor runs that might be needed per year? In either of these cases, it is important to have the flexibility to super-concentrate your feeding supplements so you get less product dilution, more space for an additional feeding, and/or more working volume to start your run (see figure). In fact, you may gain about 20% more bioreactor utilization by exploring the unique ability to super-concentrate this EfficientFeed B+ product.

Simplified Handling to Reduce Risk in Scale-Up
The ability to scale up in an AGT™ dry format provides ease of handling and storage in manufacturing and other bioprocess settings. Fewer parts and steps in reconstitution mean fewer opportunities for operator error. Further, ability to store this feed, even when super-concentrated, for the length of a bioreactor run at room temperature, in the dark, may allow you fewer chances of contamination during closed system connections by reducing the number of connections to the bioreactor to one.

Leibovitz's L-15 Medium, GlutaMAX™ Supplement Gibco™

Leibovitz's L-15 Medium supports the growth of HEP-2 monkey kidney cells and primary explants of embryonic and adult human tissue. L-15 is buffered by phosphates and free base amino acids instead of sodium bicarbonate. This medium is designed for supporting cell growth in environments without CO2 equilibration.

This L-15 is modified as follows:

WithWithout
• Galactose• Glucose
• Phenol Red• HEPES
• GlutaMAX™• Sodium Bicarbonate
• Sodium Pyruvate

The complete formulation is available.

Gibco® L-15 with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco® L-15 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

L-15 contains no proteins, lipids, or growth factors. Therefore, L-15 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).

McCoy's 5A Medium (Park and Terasaki modification) Thermo Scientific™

McCoy's 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues.

This McCoy’s 5A (modified) is provided as follows:

WithWithout
High GlucoseSodium Pyruvate
L-glutamine
Bacto-peptone
Phenol Red
The complete formulation is available.

Dr. Thomas McCoy originally formulated McCoy's 5A medium as a modification of Basal Medium 5A. Unlike other media, McCoy's 5A contains the reducing agent glutathione, bacto-peptone, and a high level of glucose. Park and Terasaki modified McCoy’s medium with modified levels of glycine, L-glutamic acid, L-hydroxyproline, L-proline, L-serine, L-threonine, L-tryptophan, and L-valine, plus the addition of antibiotics and HEPES.

Product Use
For Research Use Only. Not for use in diagnostic procedures.

cGMP Manufacturing and Quality System
Gibco™ McCoy's 5A is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Gibco™ McCoy's 5A (Park and Terasaki modification) requires serum supplementation, commonly with 10% Fetal Bovine Serum (FBS). Gibco™ McCoy's 5A (Park and Terasaki modification) uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

CTS™ Neurobasal™-A Medium Gibco™

CTS Neurobasal-A Medium is designed with the translational stem cell researcher in mind. It can be used to culture post-natal neurons without the need for an astrocyte feeder layer when used with B-27 supplements.

CTS quality
Gibco CTS products are manufactured at a site that uses methods and controls that conform with cGMP for medical devices, 21 CFR Part 820. Our FDA-registered manufacturing sites are ISO 13485– and ISO 9001–certified.

Accompanied by documentation to support your regulatory filing
Specific intended-use statements, full documentation traceability, and convenient access to our Drug Master File (DMF) are available.

For other applications, we provide alternative formulations of CTS Neurobasal media and B-27 supplements.

For established protocols using Neurobasal media, access our Gibco Neurobiology Protocol Handbook.

View publications using Gibco neurobiology products at our Gibco CellCite.

CD DG44 Medium Gibco™

CD DG44 Medium is a chemically defined, protein-free medium optimized for the growth of dihydrofolate reductase deficient (DHFR-) Chinese Hamster Ovary (CHO) cells and expression of recombinant proteins in suspension culture. CD DG44 fully chemically defined formulation contains no lysates, hydrolysates, nor proteins or peptide components of animal, plant, or synthetic origin.

When to Use CD DG44 Medium
• You use parental DG44 or DXB11 CHO cells (dhfr-)

When to Consider Another Gibco® Medium

Consider CD FortiCHO™ medium when:
• The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processing

Consider CD OptiCHO™ medium when:
• You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines
• Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
• Your CHO cell line is “finicky," i.e. hard to grow or adapt

Consider CD CHO medium when:
• Your GS CHO cells are recommended to be grown in CD CHO medium
• Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)

Design Your Own Media Specific to Your Needs
Whether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco® cGMP Media Custom Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.

CTS™ Opti-MEM™ I Medium Gibco™

Gibco CTS Opti-MEM I Medium is an improved minimal essential medium (MEM) that is recommended for use with the CTS LV-MAX Transfection Kit as a complexation buffer. This medium contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. CTS Opti-MEM I uses a sodium bicarbonate buffer system (2.4 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH. The formulation of CTS Opti-MEM I is identical to research-grade Opti-MEM I Reduced Serum Medium.

CTS Opti-MEM I is xeno-free and contains human transferrin.

CTS Opti-MEM I is manufactured in conformity with cGMP for medical devices, 21 CFR, Part 820, and certified to the ISO 13485. Drug Master File and Regulatory Support File available upon request.

Custom configuration and packaging available upon request.

PBS Tablets Gibco™

Phosphate-buffered saline (PBS) is a balanced salt formulation used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Each 5 g Gibco® PBS Tablet should be dissolved in 500 mL of distilled water. The pH will be 7.45 and requires no adjustment.

We offer a variety of Gibco® PBS formulations for a range of cell culture applications.

These PBS Tablets are manufactured as follows:
Without
• Calcium
• Magnesium
• Phenol Red

The complete formulation is available.

CD OptiCHO™ AGT™ Medium Gibco™

CD OptiCHO™ medium is specifically designed to offer high performance and yield with recombinant CHO cells in a chemically defined fed-batch environment. The protein-free, animal origin free formulation is designed to provide consistency and reduces the need to screen for adventitious agents. CD OptiCHO™ medium builds on Gibco® CD CHO’s legacy of consistent cell growth and titers, while offering up to 40% better titer and cell growth over select competitors. Thanks to its low osmolality and advanced CHO CD EfficientFeed™ Liquid Nutrient Supplement Kit feeding options, CD OptiCHO™ medium is capable of gaining 2-5 fold increases in fed-batch productivity over batch culture.

When to Use CD OptiCHO™ Medium
• You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines
• Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
• Your CHO cell line is “finicky," i.e. hard to grow or adapt

When to Consider Another Gibco® Medium

Consider CD FortiCHO™ medium when:
• The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processing

Consider CD CHO medium when:
• Your GS CHO cells are recommended to be grown in CD CHO medium
• Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)

Consider CD DG44 medium when:
• You use parental DG44 or DXB11 CHO cells (dhfr-)

Liquid Media Formulations
Gibco® AGT™ media are also available in a liquid format that offers the same performance and formulation as the AGT™ media format. Gibco® liquid media are manufactured animal origin-free with the same complete, serum-free, protein-free, chemically-defined formulation and performance as their AGT™ media counterparts.

Design Your Own Media Specific to Your Needs
Whether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco® cGMP Media Custom Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.

HuMEC Ready Medium (1X) Gibco™

Engineered specifically for culture of human mammary epithelial cells, HuMEC Medium is a serum-free formulation with frozen supplements containing growth factor and other components. This translates to greater experimental validity, more reproducible results, and reduction of lot-to-lot variability and aberrations that can occur with the addition of serum. Human mammary epithelial cells cultured in HuMEC Medium have gene expression profiles consistent with normal mammary epithelial cells, as tested on a cDNA microarray.

Applications:
HuMEC Medium is optimized specifically for the growth of human mammary epithelial cells, and has been performance tested in a growth assay using normal human mammary epithelial cells. HuMEC Medium promotes superior growth of normal human mammary epithelial cells, making it ideal for researchers in breast cancer, drug discovery, and other aspects of breast biology.

Contents and Storage:
HuMEC Ready Medium consists of HuMEC Basal Serum-Free Medium (12753018) supplemented with HuMEC Supplement Kit (12755013). The HuMEC Supplement Kit includes 5 ml of a supplement mix containing epidermal growth factor, hydrocortisone, isoproterenol, transferrin, and insulin, and 25 mg of bovine pituitary extract. Store HuMEC Ready Medium at -5°C to -20°C. Store the supplement mix at -5°C to -20°C.

StemFlex™ Medium Gibco™

Gibco™ StemFlex™ Medium supports the robust expansion of feeder-free pluripotent stem cells (PSCs) and is optimized to support novel applications, including single-cell passaging, gene editing, and reprogramming. StemFlex Medium’s unique formulation offers the convenience of a flexible feeding schedule (including weekend-free options) and also the ability to choose the matrix and passaging reagent that best suits specific applications. StemFlex Medium enables the long term feeder-free culture of PSCs without karyotypic abnormalities and maintains the cells' ability to differentiate into all three germ layers.

Superior results
StemFlex Medium is formulated to deliver superior outcomes in today’s more-challenging applications that stress PSC cultures, such as reprogramming, single cell passaging, and gene editing. StemFlex Medium provides superior cell survival compared to the leading competitor (mTeSR1), allowing for greater success in the most relevant cell types.

Flexibility
StemFlex Medium allows for unprecedented 3-way usage flexibility for PSC culture conditions: the ability to select an optimal feeding schedule, including a weekend-free option; a choice of matrices, depending upon the needs of the workflow; and the option to select the passaging reagent for the level of cell dissociation required.

Exceptional value and performance
StemFlex Medium is provided in a convenient two-component kit (450 mL basal medium & 50 mL supplement), and when used with Geltrex™ LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix, provides a cost-effective, robust system for superior feeder-free culture of human PSCs. StemFlex Medium’s ability to enable weekend-free culture of PSCs provides unmatched value among the robust feeder-fee PSC media on the market today.

HepExtend™ Media Kit Gibco™

The HepExtend™ Media Kit contains the new HepExtend Supplement (50X), as well as William's E Medium and Primary Hepatocyte Maintenance Supplement, providing the three components needed to optimize long term hepatocyte culture. HepExtend Supplement has been optimized for use with cryopreserved primary hepatocytes to improve cell viability, function and number of days in culture. This enables researchers to perform metabolic and toxicity experiments not achievable using standard culture conditions. Simply add the HepExtend Supplement and Primary Hepatocyte Maintenance Supplement to the William’s E Medium for a complete solution. The HepExtend supplement does not contain any small molecules or fetal bovine sera, components that are known to interfere with primary hepatocyte function.

Enabling—offers maximum cell viability and cell health when culturing primary plateable hepatocytes
Extended cell survival—days in culture can be extended to >10 days while maintaining normal cell morphology and bile canaliculi
Consistent—manufactured in a cGMP compliant facility using high quality materials

Maintain P450 activity
The HepExtend Media Kit can be used to help ensure metabolically competent hepatocytes for at least 10 days for use in downstream ADME/Tox applications not achieved with standard 5-day culture systems.

Enhanced gene expression
Using the components in the HepExtend Media Kit to culture primary human hepatocytes increases the basal gene expression of many key genes involved in drug metabolism and transport.

Retain inducibility
Nuclear receptor activation and gene inducibility are retained in cultures treated with HepExtend supplemented media.

LHC-8 Medium (1X) Gibco™

LHC serum-free media are the most widely used products for culture of bronchial epithelial cells. For maximum flexibility and convenience, we offer these products with or without supplementation.

LHC Basal Medium (1X), liquid

Applications:
Formulated for the growth of bronchial epithelial cells with further supplements.

Recommended storage conditions:
2° to 8°C.

Shelf life:
12 months

Intended use(s)
Research use only



LHC-8 Medium and LHC-9 Medium


Applications:


Engineered specifically for the growth of bronchial epithelial cells such as IB3-1, S9, and C38, BEAS-2B, BZR, BBM, Het-1A, NHBE (normal human bronchial epithelial cells), and BBE (bovine bronchial epithelial cells) for asthma, allergy, lung cancer, cystic fibrosis, pulmonary, and esophageal cancer research without further supplements.


Performance and quality testing:

Performance tested in a growth assay using BEAS-2B cells.

Recommended storage conditions:
-5°C to -20°C.

Intended use(s)
Research use only

Reference(s):
Pawliczak R., et. al. (2001) J Biol Chem 276, 44613. Zhao Y.L., et. al. (2000) Carcinogenesis 21, 2005. Liu X., et. al. (1998) Am J Physiol 274, L58. Hei T.K., et. al. (1997) Environ Health Perspect 105, 1085. Lechner J.F., and LaVeck, M.A. (1985) J Tissue Cult Methods 9, 43.
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