Alexa Fluor™ 488 Hydrazide is useful as a cell tracer and as a reactive dye for labeling aldehydes or ketones in polysaccharides or glycoproteins. Alexa Fluor™ 488 is a bright, green fluorescent dye with excitation ideally suited to the 488 nn laser line.
Alexa Fluor™ dyes are reactive molecules that can be used to add a fluorescent label to the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting Alexa Fluor™ conjugates exhibit brighter fluorescence and greater photostability than...
Thermo Scientific EZ-Link Sulfo-NHS-LC-LC-Biotin enables simple and efficient biotin labeling of antibodies, proteins, and any other primary amine–containing macromolecules. Specific labeling of cell surface proteins is another common application for these uniquely water-soluble and membrane...
Alexa Fluor antibody labeling kits provide a convenient means to label antibodies or proteins (>40 kDa) with as many as nine different colored dyes. Conjugated antibodies produce lower background fluorescence with less nonspecific binding and are ideal for multiplexing in a variety of applications.
Alexa Fluor™ 647 is a bright and photostable far-red dye with excitation ideally suited to the 633 nm laser line. Used for stable signal generation in imaging and flow cytometry, Alexa Fluor™ 647 dye is water soluble and pH-insensitive from pH 4 to pH 10.
Thermo Scientific EZ-Link Maleimide-PEG11-Biotin is a long, maleimide-activated, sulfhydryl-reactive biotinylation reagent that includes an 11-unit polyethylene glycol spacer arm for increased water-solubility and reach.
Molecular Probes™ Antibody Conjugate Purification kits efficiently purify antibody conjugates from unconjugated dye or biotin. This particular kit is optimized for the purification of 0.5–1 mg of antibody conjugate.
The amine-reactive quencher, dabcyl succinimidyl ester has a broad and intense visible absorption but no fluorescence making it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications.
Tandem Mass Tag™ 2-plex (TMTduplex™) Isotopic Label Reagents comprise “light” TMTzero and “heavy” TMT6-127 label reagents while Tandem Mass Tag™ 2-plex (TMTduplex) Isobaric Label Reagents comprise “heavy” TMT2-126 and TMT2-127 Label Reagents for multiplexing.
The Thermo Scientific EZ-Link Maleimide Protein Labeling Kit provides a convenient method to label proteins with varying amounts maleimide for crosslinking or conjugation with a thiol/sulfhydryl-containing moiety.
Thermo Scientific Pierce DST, also called disuccinimidyl tartrate, is a water-soluble crosslinker that contains amine-reactive NHS ester ends around a 4-atom spacer arm, whose central diol can be cleaved by periodate oxidation.
Thermo Scientific Pierce Sulfo-EGS is a water-soluble crosslinker that contains amine-reactive sulfo-NHS-ester ends around a 12-atom spacer arm, which can be cleaved by treatment with hydroxylamine at pH 8.5.
Thermo Scientific Pierce EGS is a crosslinker that contains amine-reactive NHS-ester ends around a 12-atom spacer arm, which can be cleaved by treatment with hydroxylamine at pH 8.5. Features of the EGS crosslinker: • Reactive groups: NHS ester (both ends) • Reactive towards: amino groups (primary...
Thermo Scientific DyLight 800 Amine-Reactive Dye is an NHS ester-activated derivative of high-performance DyLight 800 used to fluorescently label antibodies and other proteins that are then used as molecular probes for cellular imaging and other fluorescence detection methods.
Fluorescein isothiocyanate (FITC) is an amine-reactive derivative of fluorescein dye that has wide-ranging applications as a label for antibodies and other probes, for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA.