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Tango™ GPR119-bla U2OS DA Assay Kit

The Tango™ GPR119-bla U2OS DA cells contain the human G-protein Coupled Receptor 119 (GPR119) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ GPR119-bla U2OS DA cells have been functionally validated for Z' factor and EC50 concentrations of established ligands. In addition, Tango™ GPR119-bla U2OS DA cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary for each product. Division Arrested cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.

GeneBLAzer™ NTSR1 CHO-K1 DA Assay Kit

The GeneBLAzer® NTSR1 CHO-K1 DA cells contain the human Neurotensin Receptor 1 (NTSR1) stably integrated into the GeneBLAzer® Gqo5-NFAT-bla CHO-K1 cell line. CellSensor® NFAT-bla CHO-K1 cells (Cat. no. K1078) contain a beta-lactamase reporter gene under control of the NFAT response element.

The GeneBLAzer® NTSR1 CHO-K1 DA cells have been functionally validated for Z' factor and EC50 concentrations of established ligands. In addition, GeneBLAzer® NTSR1 CHO-K1 DA cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary for each product. Division Arrested cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.

T-REx™ Tango™ P2RY14-bla U2OS Cells

T-REx™ Tango™ P2RY14-bla U2OS cells contain the human purinergic receptor P2Y14 (P2RY14) under doxycycline inducible control linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase reporter gene under the control of a UAS response element. The T-REx™ Tango™ P2RY14-bla U2OS cells have been functionally validated for Z’ factor and EC50 concentrations of UDP-glucose. In addition, T-REx™ Tango™ P2RY14-bla U2OS cells have been tested for assay performance under variable conditions.

Tango™ GPR119-bla U2OS Cells

The Tango™ GPR119-bla U2OS cells contain the human G-protein Coupled Receptor 119 (GPR119) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ GPR119-bla U2OS cells have been functionally validated for Z' factor and EC50 concentrations of established ligands. In addition, Tango™ GPR119-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary for each product.

Tango™ GPR44 (CRTH2)-bla U2OS Cells

Tango™ GPR44-bla U2OS cells contain the human G protein coupled receptor 44 (GPR44) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase reporter gene under the control of a UAS response element. The Tango™ GPR44-bla U2OS cells have been functionally validated for Z’ factor and EC50 concentrations of Indomethacin. In addition, Tango™ GPR44-bla U2OS cells have been tested for assay performance under variable conditions.

GeneBLAzer™ LTB4R-Gα15-NFAT-bla CHO-K1 Cells

GeneBLAzer® LTB4R-Gα15-NFAT-bla CHO-K1 cells contain the human Leukotriene B4 Receptor (LTB4R), (Accession # NM_181657) stably integrated into the GeneBLAzer® Gα15-NFAT-bla CHO-K1 cell line. GeneBLAzer® Gα15-NFAT-bla CHO-K1 cells (Cat. no. K1537) contain a beta-lactamase (bla) reporter gene under control of the NFAT Response Element.

GeneBLAzer® LTB4R-Gα15-NFAT-bla CHO-K1 cells are functionally validated for Z'-factor and EC50 concentrations of LTB4. In addition, GeneBLAzer® LTB4R-Gα15-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.

Tango™ OPRD1-bla U2OS Cells

The Tango™ OPRD1 -bla U2OS cells contain the human Opioid Receptor Delta 1 (OPRD1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ OPRD1-bla U2OS cells have been functionally validated for Z' factor and EC50 concentrations of established ligands. In addition, Tango™ OPRD1-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary for each product.

Tango™ M1-bla U2OS Cells

Tango™ M1-bla U2OS cells contain the human muscarinic M1 receptor (CHRM1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase reporter gene under the control of a UAS response element. The Tango™ M1-bla U2OS cells have been functionally validated for Z’ factor and EC50 concentrations of oxotremorine M. In addition, Tango™ M1-bla U2OS cells have been tested for assay performance under variable conditions.

GeneBLAzer™ T-REx™-G2A-NFAT-bla FreeStyle™ 293F Cells

GeneBLAzer® T-REx™ G2A-NFAT-bla Freestyle HEK 293F cells contain the human GPR132 (G2A), (Accession # NP_037477) stably integrated into the CellSensor® NFAT-bla Freestyle HEK 293F cell line. CellSensor® NFAT-bla Freestyle HEK 293F cells (Cat. no. K1725) contain a beta-lactamase reporter gene under control of the NFAT Response Element.

The GeneBLAzer® T-REx™ G2A-NFAT-bla Freestyle HEK 293F cells are functionally validated for Z' and EC50 concentrations of dDAVP. In addition, GeneBLAzer® GeneBLAzer® T-REx™ G2A-NFAT-bla Freestyle HEK 293F cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

G2A is an orphan GPCR. Upon overexpession G2A is constitutively active. This is an assay for the orphan GPCR based upon constitutive activity. To use the assay to detect inverse agonists, high amounts of doxycycline should be added. To use the assay to detect agonists, low amounts of doxycycline should be added.

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.

Tango™ CXCR4-bla U2OS Cells

The Tango™ CXCR4-bla U2OS cells contain the human Chemokine (C-X-C motif) receptor 4 (CXCR4) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ CXCR4-bla U2OS cells are functionally validated for Z' and EC50 concentrations of SDF1α. In addition, Tango™ CXCR4-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.

Tango™ CCR4-bla U2OS Cells

The Tango™ CCR4-bla U2OS cells contain the human Chemokine (C-C Motif) Receptor 4 (CCR4) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ CCR4-bla U2OS cells are functionally validated for Z' and EC50 concentrations of TARC. In addition, Tango™ CCR4-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.

Tango™ MLNR-bla U2OS Cells

Tango™ MLNR-bla U2OS cells contain the human Motilin Receptor (MLNR) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase reporter gene under the control of a UAS response element. The Tango™ MLNR-bla U2OS cells have been functionally validated for Z’ factor and EC50 concentrations of Motilin.

GeneBLAzer™ ADRB3 CHO-K1 DA Assay Kit

The GeneBLAzer® ADRB3 CHO-K1 DA (Division Arrested) cells contain the human Adrenergic Beta 3 Receptor (ADRB3) (Accession # NM_000025.1) stably integrated into the CellSensor® CRE-bla CHO-K1 cell line. CellSensor® CRE-bla CHO-K1 cells (Cat #K1129) contain a beta-lactamase (bla) reporter gene under control of the Cyclic AMP Response Element (CRE).
The ADRB3 CHO-K1 DA cells are functionally validated for Z'-factor and EC50 concentrations of Isoproterenol. In addition, ADRB3-CRE-bla CHO-K1 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.

Tango™ HTR2A-bla U2OS DA Assay Kit

The Tango™ HTR2A-bla U2OS DA cells contain the human Serotonin Type 2A receptor (HTR2A) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ HTR2A-bla U2OS DA cells have been functionally validated for Z' factor and EC50 concentrations of established ligands. In addition, Tango™ HTR2A-bla U2OS DA cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary for each product. Division Arrested cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.

GeneBLAzer™ NTSR1-NFAT-bla CHO-K1 Cells

GeneBLAzer® NTSR1-NFAT-bla CHO-K1 cells contain the human Neurotensin Receptor 1 (NTSR1) stably integrated into the CellSensor® NFAT-bla CHO-K1 cell line. CellSensor® NFAT-bla CHO-K1 cells (Cat. no. K1078) contain a beta-lactamase reporter gene under control of the NFAT.

The GeneBLAzer® NTSR1-NFAT-bla CHO-K1 cells have been functionally validated for Z' factor and EC50 concentrations of established ligands. In addition, GeneBLAzer® NTSR1-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary for each product.