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KaryoMAX™ Colcemid™ Solution in PBS (Gibco™)

Gibco® KaryoMAX® Colcemid™ Solution in PBS is a 10 µg/ml N-desacetyl-N-methylocolchicine (Colcemid™) solution made in Phosphate Buffered Saline (PBS). It is used for arresting cells in metaphase so the chromosomes can be separated for cytogenetic studies and in vitro diagnostic procedures. Colcemid™ prevents spindle formation during mitosis, causing metaphase arrest. The mechanism of action is similar to that of colchicine, but with lower mammalian toxicity. The complete formulation is available.

Product Use
For in vitro diagnostic use.

cGMP Manufacturing and Quality System
Gibco® KaryoMAX® Colcemid™ Solution in PBS is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Phytohemagglutinin, M form (PHA-M) (Gibco™)

Gibco® Phytohemagglutinin, M form (PHA-M) is a solution made from a crude extract of the red kidney bean Phaseolus vulgaris. This extract is capable of inducing blastogenesis in vitro in various mammalian mononuclear cells when used at a concentration of 1–2% v/v. Each lot of Gibco® Phytohemagglutinin is performance tested at 1.5% using peripheral blood lymphocytes from a normal adult donor cultured for 72 hours in Gibco® RPMI 1640 Medium supplemented with Fetal Bovine Serum (FBS), heparin, antibiotics, and L-glutamine.

cGMP Manufacturing and Quality System
Gibco® Phytohemagglutinin is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

KaryoMAX™ Colcemid™ Solution in HBSS (Gibco™)

Gibco® KaryoMAX® Colcemid™ Solution in HBSS is a 10 µg/mL N-desacetyl-N-methylocolchicine (Colcemid™) solution made up in Hanks' Balanced Salt Solution (HBSS). Colcemid™ prevents spindle formation during mitosis, arresting cells in metaphase so that the chromosomes can be separated for cytogenetic studies and in vitro diagnostic procedures. The mechanism of action is similar to that of colchicine, but with lower mammalian toxicity.

The complete formulation is available.

cGMP Manufacturing and Quality System
Gibco® KaryoMAX® Colcemid™ Solution in HBSS is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

eBioscience™ Phytohemagglutinin-L (PHA-L) Solution (500X) (Invitrogen™)

The Phytohemagglutinin-L (PHA-L) Solution (500X) is a ready to use solution of PHA-L in aqueous buffer. PHA is a mixture of lectins isolated from the red kidney bean, Phaseolus vulgaris. PHA-L is the fraction of PHA that exhibits strong leukoagglutinating and mitogenic activity, but very little erythroagglutinating activity. This reagent is intended for use in in vitro activation of human and mouse leukocytes.

Reported Application
Functional Assays

KaryoMAX™ Giemsa Stain Solution (Gibco™)

Gibco® KaryoMAX® Giemsa Stain Solution is a solution used for G-banding of chromosomes for cytogenetic analysis. Gibco® KaryoMAX® Giemsa Stain Solution is formulated as 6.0 g Azur II Eosin and 1.6 g Azur II per liter in glycerol/methanol.

Product Use
For in vitro diagnostic use.

cGMP Manufacturing and Quality System
Gibco® KaryoMAX® Giemsa Stain Solution is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Gurr Buffer Tablets (Gibco™)

Gibco® Gurr Buffer Tablets offer a convenient way to prepare a pH 6.8 phosphate buffer for dilution of Giemsa stain used in G-banding of chromosomes for cytogenetic analysis. Simply add one buffer tablet to one liter of distilled water and stir until dissolved.

KaryoMAX™ Potassium Chloride Solution (Gibco™)

Gibco® KaryoMAX® Potassium Chloride Solution is a 0.075 M hypotonic solution for enlarging cells to facilitate the spreading of metaphase chromosomes for karyotyping. Gibco® KaryoMAX® Potassium Chloride Solution is formulated as 5.592 g potassium chloride per liter distilled water.

cGMP Manufacturing and Quality System
Gibco® KaryoMAX® Potassium Chloride Solution is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

AmnioMAX™ C-100 Supplement (Gibco™)

Gibco® AmnioMAX™ C-100 Supplement is part of a kit used to prepare a fully-supplemented Gibco® AmnioMAX™ C-100 Complete Medium developed for the short term culture of human amniotic fluid cells for cytogenetic studies and in vitro diagnostic procedures.

Gibco® AmnioMAX™ C-100 Complete Medium features:

• Easy-to-use format
• Quality and performance testing
• Unique, optimized formulation

Easy-to-use format
Gibco® AmnioMAX™ C-100 Complete Medium is a two-part kit for preparation of a complete medium that requires no additional supplementation. The complete medium can be stored at 2–8°C for up to ten days.

Quality and performance testing
Every lot of Gibco® AmnioMAX™ C-100 Complete Medium is performance tested by a certified US reference cytogenetics laboratory to ensure consistently superior performance. Pooled primary human amniotic fluid samples are cultured for six days in Gibco® AmnioMAX™ C-100 Complete Medium before measuring the total number of colonies and total number of mitotic colonies. In addition, each lot is tested for sterility, pH and osmolality.

Unique, optimized formulation
Gibco® AmnioMAX™ C-100 Complete Medium contains Fetal Bovine Serum (FBS), gentamicin, and L-glutamine to maximize cell attachment and growth. This optimized medium also has an enhanced buffering system that provides greater pH stability during culture manipulations.

Product Use
For in vitro diagnostic use.

cGMP Manufacturing and Quality System
Gibco® AmnioMAX™ C-100 Supplement is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.