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Transferrin, Bovine (Holo form), lyophilized (Gibco™)

>90% iron saturated.

Suggested range for supplementation: 0.5 to 50 µg/ml.

Origin:
New Zealand

GlycanTune™ C+ Total Feed (Gibco™)

GlycanTune™ C+ Total Feed is an AGT™, or Advanced Granulation Technology™, feeding supplement specifically designed for a fed-batch culture of multiple cell lines. When used, this dry-format, single-part total feeding supplement can shift glycan profiles from heavily G0F to mostly G1F and G2F glycans. The product is an animal origin–free, chemically-defined formulation that contains no proteins, hydrolysates, or components of unknown composition. GlycanTune C+ Total Feed provides an easy-to-reconstitute, pH-neutral solution that can be concentrated from the suggested 87.7 g/L (1X) up to 175.4 g/L (2X). As a result, key nutrients can be delivered in smaller volumes.

Which GlycanTune Total Feed to choose?

Consider GlycanTune C+ Total Feed when:
• You have used Gibco™ EfficientFeed™ C+ (Plus) AGT Supplement in the past and wish to maintain the benefits of simpler reconstitution and super-concentration in delivering key components alongside improved glycan profile
• You are currently using another feed and wish to shift glycan profile more toward G1 and G2
• You are using a cell line or base medium where Dynamis™ Medium may be considered an option

Consider GlycanTune A+ Total Feed when:
• You have used Gibco EfficientFeed A+ (Plus) AGT Supplement in the past and wish to maintain the benefits of simpler reconstitution and super-concentration in delivering key components alongside improved glycan profile
• You are currently using another feed and wish to shift glycan profile more toward G1 and G2
• You are using a cell line or base medium where CD OptiCHO™ Medium may be considered an option

Consider GlycanTune B+ Total Feed when:
• You have used Gibco EfficientFeed B+ (Plus) AGT Supplement in the past and wish to maintain the benefits of simpler reconstitution and super-concentration in delivering key components alongside improved glycan profile
• You are currently using another feed and wish to shift glycan profile more toward G1 and G2
• You are using a cell line or base medium where CD CHO Medium may be considered an option

Dialing in a glycan profile
By following the protocol recommended to test with GlycanTune Total Feed, it is possible to shift the glycan protein expression profiles from primarily G0 to primarily G1 and G2.

Increasing titers
As a complete feed, GlycanTune total feeds have been shown to provide multiple-fold increases in titers at 1X concentration versus batch culture; as such, they are an excellent starting point for process development when a shift in glycan profile is desired. Flexibility to further increase titers is enhanced with the ability to super-concentrate the product by reconstituting with less water to deliver up to 175.4 g/L of fed nutrients (see figure).

Maximizing bioreactor utilization
Have you ever wished you could have more working volume at the start of a bioreactor run to get more protein produced, but needed too much feeding volume space to accomplish your optimal goal? Have you ever thought that room for a little more feeding might get you to a new level of titer from a process and maybe even reduce the number of bioreactor runs that might be needed per year? In either of these cases, it is important to have the flexibility to super-concentrate your feeding supplements so you get less product dilution, more space for an additional feeding, and/or more working volume to start your run (see figure). In fact, you may gain about 20% more bioreactor utilization by exploring the unique ability to super-concentrate this GlycanTune C+ product.

Simplified handling to reduce risk in scale-up
The ability to scale up using AGT dry format provides easier handling and storage in manufacturing and other bioprocess settings. Fewer parts and reconstitution steps mean fewer opportunities for operator error. Further, the ability to store this feed, even when super-concentrated, for the length of a bioreactor run at room temperature in the dark may allow less chance of contamination by reducing the number of connections to the bioreactor to one or two—depending on whether GlycanTune Total Feed can be used alone or after a change from your current feed.

MEM Non-Essential Amino Acids Solution (100X) (Gibco™)

Gibco® MEM Non-Essential Amino Acids are used as a supplement for cell culture medium, to increase cell growth and viability. Gibco® MEM Non-Essential Amino Acids are formulated to contain 100X the non-essential amino acids found in the standard Minimum Essential Medium (MEM). The complete formulation is available.

Dual-site cGMP Manufacturing and Quality System
Gibco® MEM Non-Essential Amino Acids Solution is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco® MEM Non-Essential Amino Acids Solution product made in our Scotland facility (11140-035). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Insulin-Transferrin-Selenium-Sodium Pyruvate (ITS-A) (100X) (Gibco™)

ITS-A (Insulin-Transferrin-Selenium-Sodium Pyruvate) Supplement is used as a basal medium supplement in order to reduce the amount of Fetal Bovine Serum (FBS) used to culture cells. ITS-A is used for adherent cell cultures. Originally used with RPMI 1640 and Minimum Essential Medium (MEM), ITS-A is now routinely used with other basal media to support a wide variety of cell types in the presence of 2–4% FBS. We also offer ITS-G and ITS-X supplements.

Using ITS-A Supplement
Insulin promotes glucose and amino acid uptake, lipogenesis, intracellular transport, and the synthesis of proteins and nucleic acids. Transferrin is an iron carrier and it may also help to reduce toxic levels of oxygen radicals and peroxide. Selenite is a co-factor for glutathione peroxidase and other proteins, and is used as an anti-oxidant in media. Sodium Pyruvate is a precursor to amino acids, fatty acids, and cholesterol; it is an important intermediate in many biosynthetic pathways. The complete formulation is available.

cGMP manufacturing and quality system
ITS-A Supplement is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

EfficientFeed™ A+ AGT™ Supplement (Gibco™)

Gibco® EfficientFeed™ A+ (Plus) AGT™ Supplement is a dry-format single-part supplement for fed batch addition to the culture of multiple cell lines to assist with process development and maximization of bioreactor utilization. Compared to original CHO CD EfficientFeed™ A AGT™ nutrient supplement, EfficientFeed™ A+ supplement has components that are uniquely combined to allow simpler single-part pH-neutral reconstitution, as well as the ability to super-concentrate the supplement from 66.4 g/L (1X) to 199.2 g/L (3X) for reduction of product dilution or increased titers with additional feeding, when appropriate. The product is an animal origin-free, chemically defined formulation that contains no proteins, hydrolysates, or components of incompletely defined composition.

Which EfficientFeed™ + Supplement to Choose?

Consider EfficientFeed™ A+ AGT™ Supplement when:
• You have used CHO CD EfficientFeed™ A supplement in the past and wish to explore simpler reconstitution and super-concentration benefits of new technology for delivery of key components
• You are currently using CD OptiCHO™ as your base cell growth medium

Consider EfficientFeed™ B+ AGT™ Supplement when:
• You have used CHO CD EfficientFeed™ B supplement in the past and wish to explore simpler reconstitution and super-concentration benefits of new technology for delivery of key components
• You are currently using CD CHO as your base cell growth medium

Consider EfficientFeed™ C+ AGT™ Supplement when:
• You have used CD EfficientFeed™ C supplement in the past and wish to explore simpler reconstitution and super-concentration benefits of new technology for delivery of key components
• You want higher productivity in GS CHO, CHO-K1, & CHO-S® derived cell lines
• You are currently using CD FortiCHO™ as your base cell growth medium
• You are using a base medium with greater than 6 g/L glucose or a hydrolysate at day 0

Increasing Titers
As a complete feed, EfficientFeed™ + supplements have been shown to provide multiple-fold increases in titers at 1X concentration versus batch culture, and are an excellent starting point for process development. Flexibility to further increase titers are enhanced with the ability to super-concentrate the product by reconstituting with less water to deliver up to 199 g/L of fed nutrients (see figure).

Maximizing Bioreactor Utilization
Have you ever wished you could have more working volume at the start of a bioreactor run to get more protein produced, but needed too much feeding volume space to accomplish your optimal goal? Have you ever thought that room for a little more feeding might get you to a new level of titer from a process and maybe even reduce the number of bioreactor runs that might be needed per year? In either of these cases, it is important to have the flexibility to super-concentrate your feeding supplements so you get less product dilution, more space for an additional feeding, and/or more working volume to start your run (see figure). In fact, you may gain about 20% more bioreactor utilization by exploring the unique ability to super-concentrate this EfficientFeed A+ product.

Simplified Handling to Reduce Risk in Scale-Up
The ability to scale up in an AGT™ dry format provides ease of handling and storage in manufacturing and other bioprocess settings. Fewer parts and steps in reconstitution mean fewer opportunities for operator error. Further, ability to store this feed, even when super-concentrated, for the length of a bioreactor run at room temperature, in the dark, may allow you fewer chances of contamination during closed system connections by reducing the number of connections to the bioreactor to one.

KnockOut™ Serum Replacement (Gibco™)

Gibco™ KnockOut™ Serum Replacement (KnockOut™ SR) is a more defined, FBS-free medium supplement that supports the growth of pluripotent stem cells (PSCs) cultured on fibroblast feeder cells. Designed to replace fetal bovine serum (FBS) in existing protocols, this serum replacement has been trusted by many for almost 20 years. Providing the documents required for regulatory review, KnockOut SR reduces your burden in qualifying reagents when transitioning from research applications to clinical applications. If you are interested in only basic research applications, see the Research Use Only version of this product to ensure that you receive the benefits and pricing that fit your needs.

With KnockOut Serum Replacement, you can:
• Culture feeder-dependent PSCs with confidence
• Rely on the improved performance of a qualified, FBS-free formulation
• Enjoy the peace of mind of having a more stable price and supply compared to FBS

Culture PSCs with confidence
KnockOut SR has been cited in more than 2000 publications. It has been shown to support feeder-dependent culture of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). It has also been shown to support reprogramming, embryoid body (EB) formation, in vitro differentiation, cryopreservation, ESC derivation, and many other applications.

Rely on improved performance
KnockOut SR has more a defined formulation compared to FBS, which contains many undefined and variable components. Unlike most FBS products, each lot is tested for its ability to support PSCs. Because of this, culturing PSCs with KnockOut SR leads to less differentiation and more compact colony morphology compared to FBS.

Enjoy the peace of mind of having stable price and supply
KnockOut SR is not affected by the limited supply and fluctuating prices often seen with FBS. This makes it easier to plan your budget and buy what you need, especially since it is more affordable than most FBS products.



This reagent is classified as a medical device in several countries. If it is not regulated as a medical device in a specific region, it should be used for laboratory use only.

EpiLife™ Defined Growth Supplement (EDGS) (Gibco™)

A defined, sterile, concentrated (100X) solution intended for use with EpiLife® medium to culture human epidermal keratinocytes. EDGS is not intended for use with Medium 154. EDGS contains all of the growth factors and hormones necessary for the growth of human epidermal keratinocytes. Each bottle contains the correct amount of supplement for a 500 ml bottle of EpiLife® medium. EDGS contains:
• purified bovine serum albumin
• purified bovine transferrin
• hydrocortisone
• recombinant human insulin-like growth factor type-1 (rhIGF-1)
• prostaglandin E2 (PGE2)
• recombinant human epidermal growth factor (rhEGF) The bovine products in EDGS have been isolated from animals of North American origin. The isolation process for these components includes steps that inactivate viruses. All components of EDGS are greater than 95% pure.

GlycanMod™ G175 Supplement (Gibco™)

GlycanMod G175 is a chemically defined protein quality supplement, enriched in carbohydrates and trace elements, that is designed to increase mAb galactosylation across multiple cell lines. It is also available as part of the GlycanMod Pak, which includes one of each of our GlycanMod supplements in 100-g, non-GMP sizes.

Each protein quality supplement has a distinct composition which provides more options for achieving your target profile. These supplements are titratable and can be added as needed to achieve the desired galactosylation pattern. GlycanMod supplements offer a solution for your galactosylation needs, helping to get your product to market faster.

The GlycanMod Pak includes:
• GlycanMod G100—enriched in micronutrients
• GlycanMod G125—enriched in carbohydrates and micronutrients
• GlycanMod G150—enriched in trace elements
• GlycanMod G175—enriched in carbohydrates and trace elements

CTS™ B-27™ Supplement, XenoFree, minus insulin (Gibco™)

CTS B-27 Supplement XenoFree Minus Insulin is based on the classic B-27 Supplement formulation and contains no animal-derived components at the primary component level. The absence of insulin makes this formulation ideal for the growth of hippocampal and other CNS neurons in applications where the presence of insulin causes interference or in the derivation of cardiomyocytes and mesodermal cells from stem cells. CTS B-27 Supplement XenoFree Minus Insulin reduces the burden in qualifying reagents when transitioning from the research bench to the clinic.

CTS quality
Gibco CTS products are manufactured at a site that uses methods and controls that conform with cGMP for medical devices, 21 CFR Part 820. Our FDA-registered manufacturing sites are ISO 13485– and ISO 9001–certified.

Formulated xeno-free
The preparation contains no animal-derived components at the primary component level.

Accompanied by documentation to support your regulatory filing
Specific intended-use statements, full documentation traceability, and convenient access to our Drug Master File (DMF) are available.

For other applications, we provide alternative formulations of CTS B-27 supplements and CTS Neurobasal media.

For established protocols using B-27 Supplement, access our Gibco Neurobiology Protocol Handbook.

View publications using Gibco neurobiology products at our Gibco CellCite.

Feed Kit A+ (Gibco™)

The Feed Kit A+ provides two chemically defined supplements, GlygcanTune™ A+ Total Feed and EfficientFeed™ A+ Supplement, along with fed-batch supplementation strategies designed to optimize cell growth and protein production while offering the ability to target and fine tune glycosylation profiles. GlycanTune A+ Total Feed is based on the chemically-defined EfficientFeed A+ Supplement and is specifically designed for fed-batch cultures of multiple cell lines. When used alone or with EfficientFeed+ supplement, this single-part total feeding supplement can shift glycan profiles from heavily G0F to mostly G1F and G2F glycans. GlycanTune A+ Total Feed provides an easy-to-reconstitute, pH-neutral solution that can be super-concentrated for delivery of key nutrients at smaller volumes.

Dialing in a glycan profile
The manual included in the kit (and downloadable below) provides complete protocols for testing GlycanTune A+ Total Feed with EfficientFeed A+ Supplement or another feed to obtain a set of conditions similar to those shown in the charts below. The first chart depicts protocol conditions for establishing a glycan shift pattern and the second shows typical results from a shifting experiment to help you hone in on the best feeding strategy to achieve a desired galactosylation profile.

Simplified handling to reduce risk in scale-up
The ability to scale up using AGT dry format provides easier handling and storage in manufacturing and other bioprocess settings. Fewer parts and reconstitution steps mean fewer opportunities for operator error. Further, the ability to store this feed, even when super-concentrated, for the length of a bioreactor run at room temperature in the dark may lessen the chance of contamination by reducing the number of connections to the bioreactor to one or two. Customization of other feeding formulations is possible should that option suit your needs better after trying this kit. Contact your local Field Application Scientist through your Account Manager to learn more about our customization capabilities.

KnockOut™ Serum Replacement - Multi-Species (Gibco™)

Gibco™ KnockOut™ Serum Replacement – Multi-Species (KnockOut SR – Multi-Species) is a more defined, FBS-free medium supplement that supports the growth of pluripotent stem cells (PSCs) cultured on fibroblast feeder cells. Designed to replace fetal bovine serum (FBS) in existing protocols, this Research Use Only product uses the same KnockOut Serum Replacement formulation that has been trusted by many for almost 20 years. For pre-clinical or clinical applications, please see Cat. No. 10828010.

With KnockOut Serum Replacement – Multi-Species, you can:
• Culture mouse, human, and other mammalian feeder-dependent PSCs with confidence
• Rely on the improved performance of a qualified, FBS-free formulation
• Enjoy the peace of mind of having a more stable price and supply compared to FBS

Culture mammalian PSCs with confidence
The KnockOut SR – Multi-Species formulation has been cited in more than 2000 publications. It has been shown to support feeder-dependent culture of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) from mouse, human, and other mammalian species. For mouse and human PSCs, it has also been shown to support reprogramming, embryoid body (EB) formation, in vitro differentiation, cryopreservation, ESC derivation, and many other applications.

Rely on improved performance
KnockOut SR – Multi-Species has a more defined formulation compared to FBS, which contains many undefined and variable components. Unlike most FBS products, each lot is tested for its ability to support PSCs. Because of this, culturing mouse and human PSCs with KnockOut SR – Multi-Species leads to less differentiation and more compact colony morphology compared to FBS.

Enjoy the peace of mind of having stable price and supply
KnockOut SR – Multi-Species is not affected by the limited supply and fluctuating prices often seen with FBS. This makes it easier to plan your budget and buy what you need, especially since it is more affordable than most FBS products.

FunctionMAX™ TiterEnhancer, AGT™ additive (Gibco™)

FunctionMAX™ TiterEnhancer uses a new technology to combine key nutrients in a highly concentrated (150 g/L), pH-neutral, chemically-defined, AOF (animal origin-free) functional additive that is designed to reinforce existing feeds and amplify the productivity of standard high cell density fed-batch platforms. FunctionMAX™ TiterEnhancer is available in both a liquid format and this AGT™ dry format and is stable for over 1 year. FunctionMAX™ TiterEnhancer offers:

• Up to double prior fed-batch titers
• Highly concentrated ingredients
• Easy to use pH-neutral format
• Specialized delivery of ingredients found in base media—nothing exotic
• Use that is economical

When to Choose FunctionMAX™ TiterEnhancer:

• If you have a drop off in specific productivity late in a high density fed-batch culture, FunctionMAX™ TiterEnhancer may be able to improve your titers
• If you have an extremely low peak cell density fed-batch culture, you may want to consult with PD-Direct® Services for advice on cell adaptation and/or fed-batch optimization
• If you have no drop off in specific productivity late in a high density fed-batch culture and still need higher titers, you may want to consult with PD-Direct® Services for advice on fed-batch optimization

Up to Double Prior Fed-batch Titers
We have demonstrated 35–120% product titer improvements when FunctionMAX™ TiterEnhancer was added on top of existing processes or as a partial feed replacement (Figures 1–3), resulting in a lower total proportion of feed addition. These increases were achieved primarily by maintaining specific productivity throughout each fed-batch process. Improvements were seen immediately and without process optimization. We have also shown that low-volume addition of FunctionMAX™ TiterEnhancer was sufficient to improve titers for several processes using different commercially available media and feeds, with those having higher cell densities seeing the most benefit. This additive is not designed to completely replace other feeding supplements that provide additional ingredients not found in FunctionMAX™ TiterEnhancer.

Highly Concentrated Ingredients
The technology used to deliver this functional additive promises to transform recombinant drug bioprocessing by raising key ingredient concentrations to levels that are out of reach using conventional approaches. A concentration of 150 g/L is achieved with FunctionMAX™ TiterEnhancer (compared to 20–25 g/L for most basal media), and it has been shown to be stable in dry or liquid format for over 1 year.

Easy-to-Use pH-neutral Format
Reconstitution of the AGT™ dry format is surprisingly simple (add water and stir), with all components going into solution. There is no pH adjustment required, and being pH neutral it is safer in handling and use than extreme pH alternatives. As a pH neutral additive, this product will help avoid bioreactor complications often seen with extreme pH supplements. Further, the liquid is very stable—so much so that the liquid format version is made from the same AGT™ dry format material that is also available for purchase. The liquid is provided for small-scale trials and the AGT™ dry format for seamless scale-up in use.

Specialized Delivery of Ingredients Found in Base Media—Nothing Exotic
FunctionMAX™ TiterEnhancer does not introduce new ingredients to your fed-batch process; it simply provides the same key ingredients found in basal media that the cells need late in culture to maintain their productivity. Further, the technology used to combine these ingredients does not introduce any additional ingredients. Nor does it contain any growth factors or undefined components like hydrolysates. A DMF (drug master file) reference can be requested for this product for assurance of regulatory authorities that there are no substances or ingredients in the formulation that might create regulatory concerns.

Economies in Use
FunctionMAX™ TiterEnhancer is designed to deliver highly concentrated and high value addition to your fed-batch process. Consider that it provides 7-fold the ingredient concentration of a basal medium and can provide up to a doubling of titer. Further, in normal use at about 10% of working volume, the actual price per liter of use is similar to that of basal media.

B-27™ Supplement (50X), minus antioxidants (Gibco™)

A variation of the most cited neuronal cell culture supplement, B-27 Supplement Minus Antioxidants (AO) is a customized version of the original B-27 Supplement without five antioxidants (vitamin E, vitamin E acetate, superoxide dismutase, catalase, and glutathione). B-27 Supplement Minus AO is a serum-free supplement designed for studies of the role of free-radicals in aging, toxicity, apoptosis, and chronic neurologic diseases. B-27 Supplement Minus AO is provided as a 50X liquid and is intended for use with Neurobasal Medium or Neurobasal-A Medium for neuronal cell culture without the need for an astrocyte feeder layer.

B-27 Supplement is suitable for use in most neuronal cell applications. For other applications, we provide alternative formulations of B-27 Supplement and Neurobasal media.

For established protocols using B-27 Supplement, access our Gibco Neurobiology Protocol Handbook.

View publications using Gibco neurobiology products at our Gibco CellCite.

Human Keratinocyte Growth Supplement Kit (HKGS Kit) (Gibco™)

A sterile set of solutions intended for use with EpiLife® medium or Medium 154 to culture human epidermal keratinocytes. The HKGS Kit provides, in separate vials, all the components of complete HKGS (S-001-5). A vial of gentamycin/amphotericin solution (GA) is also included. Use of GA is optional.

GlutaMAX™ Supplement (Gibco™)

Gibco® GlutaMAX™ Supplement is an alternative to L-glutamine, with increased stability that improves cell health. GlutaMAX™ Supplement is suitable for both adherent and suspension culture of mammalian cells, with no adaptation required. GlutaMAX™ Supplement is offered as a 200 mM L-alanyl-L-glutamine dipeptide in 0.85% NaCl. GlutaMAX™ Supplement is also included in a variety of media formulations. Compared to L-glutamine, GlutaMAX™ Supplement:

Minimizes toxic ammonia build-up
Improves cell viability and growth
Remains stable across a wide range of temperatures

Minimizes toxic ammonia build-up
Unlike L-glutamine, GlutaMAX™ Supplement does not spontaneously break down to form ammonia. Instead, cells cleave the dipeptide bond to release L-glutamine as needed. This system prevents waste build-up and maintains a fresh supply of L-glutamine during long-term culture.

Improves cell viability and growth
Cultures with lower levels of ammonia show improved cell viability. In addition, culture conditions are more controlled due to the consistent concentration of GlutaMAX™ Supplement that is not possible with L-glutamine.

Remains stable across a wide range of temperatures
GlutaMAX™ Supplement is room-temperature stable and ready to use. GlutaMAX™ Supplement retains full functionality for 24 months at room temperature, 2 to 8°C, or -5 to -30°C for convenient storage and handling. GlutaMAX™ Supplement is even stable at 37°C for a week or more.

cGMP manufacturing and quality system
Gibco® GlutaMAX™ Supplement is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco® PBS product made in our Scotland facility (35050-038). This facility is also registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.