Shop All B-27, G-5, and N-2 Supplements

CTS™ B-27™ Supplement, XenoFree, minus insulin (Gibco™)

CTS B-27 Supplement XenoFree Minus Insulin is based on the classic B-27 Supplement formulation and contains no animal-derived components at the primary component level. The absence of insulin makes this formulation ideal for the growth of hippocampal and other CNS neurons in applications where the presence of insulin causes interference or in the derivation of cardiomyocytes and mesodermal cells from stem cells. CTS B-27 Supplement XenoFree Minus Insulin reduces the burden in qualifying reagents when transitioning from the research bench to the clinic.

CTS quality
Gibco CTS products are manufactured at a site that uses methods and controls that conform with cGMP for medical devices, 21 CFR Part 820. Our FDA-registered manufacturing sites are ISO 13485– and ISO 9001–certified.

Formulated xeno-free
The preparation contains no animal-derived components at the primary component level.

Accompanied by documentation to support your regulatory filing
Specific intended-use statements, full documentation traceability, and convenient access to our Drug Master File (DMF) are available.

For other applications, we provide alternative formulations of CTS B-27 supplements and CTS Neurobasal media.

For established protocols using B-27 Supplement, access our Gibco Neurobiology Protocol Handbook.

View publications using Gibco neurobiology products at our Gibco CellCite.

B-27™ Supplement (50X), serum free (Gibco™)

The most cited neuronal cell culture supplement, B-27 Supplement is an optimized serum-free supplement used to support the low- or high-density growth and short- or long-term viability of embryonic, post-natal, and adult hippocampal and other CNS neurons. B-27 Supplement is provided as a 50X liquid and is intended for use with Neurobasal Medium or Neurobasal-A Medium for neuronal cell culture without the need for an astrocyte feeder layer.

B-27 Supplement is suitable for use in most neuronal cell applications. For other applications, we provide alternative formulations of B-27 Supplement and Neurobasal media.

For established protocols using B-27 Supplement, access our Gibco Neurobiology Protocol Handbook.

View publications using Gibco neurobiology products at our Gibco CellCite.

B-27™ Supplement (50X), minus vitamin A (Gibco™)

A variation of the most cited neuronal cell culture supplement, B-27 Supplement Minus Vitamin A is a customized version of the original B-27 Supplement without vitamin A (retinyl acetate). B-27 Supplement Minus Vitamin A is a serum-free supplement ideal for the cultivation of neural progenitor and stem cells, either as neurospheres in suspension or in adherent monolayer culture, without inducing differentiation. B-27 Supplement Minus Vitamin A is provided as a 50X liquid and is intended for use with Neurobasal Medium or Neurobasal-A Medium for neuronal cell culture without the need for an astrocyte feeder layer.

B-27 Supplement is suitable for use in most neuronal cell applications. For other applications, we provide alternative formulations of B-27 Supplement and Neurobasal media.

For established protocols using B-27 Supplement, access our Gibco Neurobiology Protocol Handbook.

View publications using Gibco neurobiology products at our Gibco CellCite.

B-27™ Supplement, minus insulin (Gibco™)

A variation of the most cited neuronal cell culture supplement, B-27 Supplement Minus Insulin is a customized version of the original B-27 Supplement without insulin. B-27 Supplement Minus Insulin is a serum-free supplement designed to support growth of hippocampal and other CNS neurons in applications where the presence of insulin causes interference. B-27 Supplement Minus Insulin is provided as a 50X liquid and is intended for use with Neurobasal Medium or Neurobasal-A Medium.

B-27 Supplement is suitable for use in most neuronal cell applications. For other applications, we provide alternative formulations of B-27 Supplement and Neurobasal media.

For established protocols using B-27 Supplement, access our Gibco Neurobiology Protocol Handbook.

View publications using Gibco neurobiology products at our Gibco CellCite.

CTS™ B-27™ Supplement, XenoFree (Gibco™)

CTS B-27 Supplement XenoFree is based on the classic B-27 Supplement formulation and contains no animal-derived components at the primary component level. Designed with the translational stem cell researcher in mind, CTS B-27 Supplement XenoFree can be used to support induction of human neural stem cells (hNSCs) from pluripotent stem cells (PSCs), expansion of hNSCs, differentiation of hNSCs, and maintenance of mature differentiated neurons in culture.

CTS quality
Gibco CTS products are manufactured at a site that uses methods and controls that conform with cGMP for medical devices, 21 CFR Part 820. Our FDA-registered manufacturing sites are ISO 13485– and ISO 9001–certified.

Formulated xeno-free
The preparation contains no animal-derived components at the primary component level.

Accompanied by documentation to support your regulatory filing
Specific intended-use statements, full documentation traceability, and convenient access to our Drug Master File (DMF) are available.

For other applications, we provide alternative formulations of CTS B-27 supplements and CTS Neurobasal media.

For established protocols using B-27 Supplement, access our Gibco Neurobiology Protocol Handbook.

View publications using Gibco neurobiology products at our Gibco CellCite.

G-5 Supplement (100X) (Gibco™)

G-5 Supplement is a chemically defined, serum-free supplement based on Bottenstein’s G-5 formulation. G-5 Supplement is intended for use with Neurobasal® media.

G-5 Supplement is recommended for growth and expression of glial cells (normal and tumor) of astrocytic phenotype (astrocytes). G-5 Supplement can be used as a substitute for Bottenstein’s G-2 formulation. G-5 Supplement is provided as a 100X concentrate.

G-5 Supplement may be used with Neurobasal® media, DMEM, DMEM⁄F-12, or other basal media..

Learn more about G-5 Supplement formulation.

Learn more about neural cell culture products from Invitrogen at www.invitrogen.com/neuralculture.

For Research Use Only. Not intended for any animal or human therapeutic of diagnostic use.

N-2 Supplement (100X) (Gibco™)

N-2 Supplement is a chemically defined, serum-free supplement based on Bottenstein’s N-1 formulation. N-2 Supplement is intended for use with Neurobasal® media.

N-2 Supplement is recommended for growth and expression of neuroblastomas as well as post-mitotic neurons in primary cultures from both the peripheral nervous system (PNS) and the central nervous system (CNS). N-2 Supplement can be used as a substitute for Bottenstein’s N-1 formulation. N-2 Supplement is provided as a 100X concentrate.

N-2 Supplement may be used with Neurobasal® media supplemented with growth factors such as bFGF and EGF or used with DMEM.

Learn more about N-2 Supplement formulation.

Learn more about neural cell culture products from Invitrogen at www.invitrogen.com/neuralculture.

For Research Use Only. Not intended for any animal or human therapeutic of diagnostic use.

CTS™ B-27™ Supplement, XenoFree, minus vitamin A (Gibco™)

CTS B-27 Supplement XenoFree Minus Vitamin A is based on the classic B-27 Supplement formulation and contains no animal-derived components at the primary component level. The absence of vitamin A (retinyl acetate) makes this formulation ideal for the cultivation of neural progenitor and stem cells, either as neurospheres in suspension or in adherent monolayer culture, without inducing differentiation. CTS B-27 Supplement XenoFree Minus Vitamin A reduces the burden in qualifying reagents when transitioning from the research bench to the clinic.

CTS quality
Gibco CTS products are manufactured at a site that uses methods and controls that conform with cGMP for medical devices, 21 CFR Part 820. Our FDA-registered manufacturing sites are ISO 13485– and ISO 9001–certified.

Formulated xeno-free
The preparation contains no animal-derived components at the primary component level.

Accompanied by documentation to support your regulatory filing
Specific intended-use statements, full documentation traceability, and convenient access to our Drug Master File (DMF) are available.

For other applications, we provide alternative formulations of CTS B-27 supplements and CTS Neurobasal media.

For established protocols using B-27 Supplement, access our Gibco Neurobiology Protocol Handbook.

View publications using Gibco neurobiology products at our Gibco CellCite.

B-27™ Supplement (50X), custom (Gibco™)

The custom B-27® Supplement (50X) is a serum-free additive for the growth and expansion of retinal ganglion cells. It is supplied as a 50X liquid concentrate and it is used with a basal medium, such as Neurobasal®, and other additives, such as GlutaMAX-1.

B-27™ Supplement (50X), minus antioxidants (Gibco™)

A variation of the most cited neuronal cell culture supplement, B-27 Supplement Minus Antioxidants (AO) is a customized version of the original B-27 Supplement without five antioxidants (vitamin E, vitamin E acetate, superoxide dismutase, catalase, and glutathione). B-27 Supplement Minus AO is a serum-free supplement designed for studies of the role of free-radicals in aging, toxicity, apoptosis, and chronic neurologic diseases. B-27 Supplement Minus AO is provided as a 50X liquid and is intended for use with Neurobasal Medium or Neurobasal-A Medium for neuronal cell culture without the need for an astrocyte feeder layer.

B-27 Supplement is suitable for use in most neuronal cell applications. For other applications, we provide alternative formulations of B-27 Supplement and Neurobasal media.

For established protocols using B-27 Supplement, access our Gibco Neurobiology Protocol Handbook.

View publications using Gibco neurobiology products at our Gibco CellCite.

CTS™ (Cell Therapy Systems) N-2 Supplement (Gibco™)

Cell Therapy Systems N-2 CTS™ (100X) Supplement is a serum-free supplement for the growth and expression of post-mitotic neurons and tumor cells of neuronal phenotype. Cell Therapy Systems N-2 CTS™ (100X) supplement is a chemically defined, 100X concentrate of Bottenstein's N-1 formulation.

Serum-free supplement to be used with Neurobasal® CTS™ Media

• Substitute for Bottenstein’s N-1 formulation.
• Be ready for clinical research with this cGmP manufactured formula and support documentation

Use with Neurobasal® CTS™ Media for Nueronal Cell Culturing
Cell Therapy Systems N-2 CTS™ (100X) Supplement is recommended for the growth and expression of neuroblastomas and for the survival and expression of post-mitotic neurons in primary cultures from both the peripheral nervous system (PNS) and the central nervous system (CNS). Cell Therapy Systems N-2 CTS™ (100X) Supplement is intended for use with Cell Therapy Systems Neurobasal® CTS™ media.

Selective for Neuronal Cell Lines
It can be used as a substitute for the N1 Bottenstein formulation. Cell Therapy Systems N-2 CTS™ (100X) supplement appears to be selective for neuronal cell lines and does not support the growth of nonneuronal cell lines.

Media Formulation Details
N-2 supplement is a chemically defined, 100X concentrate of Bottenstein's N-2 formulation. For component concentrations of the media supplement see our technical resource page for the N-2 supplement.

Simplify Your Transition into Clinical Research
The Gibco® Cell Therapy Systems (CTS™) brand offers a broad array of products for use in cell therapy applications, including media, reagents, growth factors, enzymes, cell selection/expansion reagents, and devices. Gibco® Cell Therapy Systems (CTS™) products deliver:
• Reduced burden in qualifying reagents
• Included appropriate certificates of analyses and origin
• Convenient access to our Drug Master File (DMF)

For Research Use or Non-Commercial Manufacturing of Cell-Based Products for Clinical Research.

CAUTION: Not intended for direct administration to humans or animals