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CTS™ Immune Cell SR (Gibco™)

CTS™ Immune Cell Serum Replacement (SR) is designed to support expansion of in vitro cultured human immune cells such as T cells when added to a basal cell culture media such as CTS™ OpTmizer™ T cell Expansion SFM or CTS™ AIM-V® SFM. CTS™ Immune Cell SR is a defined xeno-free formulation and does not contain bovine or other non-human, animal-derived components.

• Minimizes the supply and safety risks associated with human serum
• Supports expansion of polyclonal T cells (CD3/CD28 bead-activated, OKT3 mAb-activated, virus-specific and tumor-specific T cells)
• Supports T cell phenotype (CD4, CD8, and CD62L) similar to human serum
• Supports expansion of gene-modified CAR T cells (lentiviral transduction)
• Reduces the cost and time associated with qualifying human serum
• Generic enough to use with the base media of your choice

Security of supply and safety
CTS™ Immune Cell SR is a defined xeno-free formulation that contains no bovine or other non-human, animal-derived components at the primary component level. The site is FDA registered and has an ISO13485-certified quality management system. The methods and controls used for the manufacturing are in conformity with current Good Manufacturing Practices for medical devices, 21 CFR Part 820, of the regulation. Full traceability documentation such as Certificate of Origin, Certificate of Analysis, and Drug Master File are available. The product can be scaled to meet future clinical and commercial demand. Alternate manufacturing sites are available as further risk mitigation.

T cell qualified
CTS™ Immune Cell SR can replace human serum at similar concentrations and achieve similar performance. CTS™ Immune Cell SR supports expansion of polyclonal T cells (CD3/CD28 bead-activated and TILs), T Cell phenotypes (CD4, CD8 and CD62L), and expansion of gene-modified CAR T cells (lentiviral transduction). Every lot is pre-qualified in a functional T cell assay using CTS™ Dynabeads® CD3/CD28-activated primary T cells to ensure consistent performance. This reduces the cost and time associated with human serum lot testing.

Versatility
CTS™ Immune Cell SR can replace human serum at similar concentrations without sacrificing performance in several different base media such as CTS™ OpTmizer™ T Cell Expansion SFM and CTS™ AIM-V® SFM or other commercial media designed for immune cells. Varying dilutions of CTS™ Immune Cell SR should be tested to determine the optimal concentration needed for your cell type and basal media.

What you get
CTS™ Immune Cell SR is available in a 50 mL bottle for small volume users as well as a 500 mL bottle for larger volume users. For added convenience, the product can be freeze-thawed once if smaller aliquots are needed. CTS™ Immune Cell SR can be added to your standard base media at similar concentrations as human serum.

CTS™ KnockOut™ SR XenoFree Kit (Gibco™)

GIBCO® KnockOut™ SR XenoFree CTS™ enables the growth and expansion of human embryonic stem cells (hESC) and human induced pluripotent stem cells (hiPSC) in a cell culture medium containing only human-derived or human recombinant proteins, which facilitates the transition of hESC and hiPSC research from the bench to the clinic.

The GIBCO® CTS™ product line enables you to reduce your burden in qualifying reagents during your transition from research applications to clinical applications.


• Defined, xenofree medium for hESC⁄iPSC stem cell culture.
• First-to-market xenofree hESC⁄iPSC product intended for Ex Vivo tissue and cell culture processing applications.


GIBCO® KnockOut™ SR XenoFree CTS™ does not contain bovine or other non-human, animal-derived components. Besides pluripotent stem cell culture expansion and maintenance, KnockOut™ SR XenoFree CTS™ can be used for hESC⁄hiPSC cryopreservation, derivation, and differentiation studies.


GIBCO® CTS™ products are of high quality, and are supplied with harmonized documentation such as Certificates of Analysis, Certificates of Origin, and access to authorization letters for our Drug Master Files, as appropriate.
The KnockOut™ SR XenoFree CTS™ Kit includes a bottle of KnockOut™ SR XenoFree CTS™ as a frozen liquid in a 500mL or a 100 mL PET bottle and a product manual.

KnockOut™ Serum Replacement (Gibco™)

Gibco™ KnockOut™ Serum Replacement (KnockOut™ SR) is a more defined, FBS-free medium supplement that supports the growth of pluripotent stem cells (PSCs) cultured on fibroblast feeder cells. Designed to replace fetal bovine serum (FBS) in existing protocols, this serum replacement has been trusted by many for almost 20 years. Providing the documents required for regulatory review, KnockOut SR reduces your burden in qualifying reagents when transitioning from research applications to clinical applications. If you are interested in only basic research applications, see the Research Use Only version of this product to ensure that you receive the benefits and pricing that fit your needs.

With KnockOut Serum Replacement, you can:
• Culture feeder-dependent PSCs with confidence
• Rely on the improved performance of a qualified, FBS-free formulation
• Enjoy the peace of mind of having a more stable price and supply compared to FBS

Culture PSCs with confidence
KnockOut SR has been cited in more than 2000 publications. It has been shown to support feeder-dependent culture of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). It has also been shown to support reprogramming, embryoid body (EB) formation, in vitro differentiation, cryopreservation, ESC derivation, and many other applications.

Rely on improved performance
KnockOut SR has more a defined formulation compared to FBS, which contains many undefined and variable components. Unlike most FBS products, each lot is tested for its ability to support PSCs. Because of this, culturing PSCs with KnockOut SR leads to less differentiation and more compact colony morphology compared to FBS.

Enjoy the peace of mind of having stable price and supply
KnockOut SR is not affected by the limited supply and fluctuating prices often seen with FBS. This makes it easier to plan your budget and buy what you need, especially since it is more affordable than most FBS products.



This reagent is classified as a medical device in several countries. If it is not regulated as a medical device in a specific region, it should be used for laboratory use only.

KnockOut™ Serum Replacement - Multi-Species (Gibco™)

Gibco™ KnockOut™ Serum Replacement – Multi-Species (KnockOut SR – Multi-Species) is a more defined, FBS-free medium supplement that supports the growth of pluripotent stem cells (PSCs) cultured on fibroblast feeder cells. Designed to replace fetal bovine serum (FBS) in existing protocols, this Research Use Only product uses the same KnockOut Serum Replacement formulation that has been trusted by many for almost 20 years. For pre-clinical or clinical applications, please see Cat. No. 10828028.

With KnockOut Serum Replacement – Multi-Species, you can:
• Culture mouse, human, and other mammalian feeder-dependent PSCs with confidence
• Rely on the improved performance of a qualified, FBS-free formulation
• Enjoy the peace of mind of having a more stable price and supply compared to FBS

Culture mammalian PSCs with confidence
The KnockOut SR – Multi-Species formulation has been cited in more than 2000 publications. It has been shown to support feeder-dependent culture of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) from mouse, human, and other mammalian species. For mouse and human PSCs, it has also been shown to support reprogramming, embryoid body (EB) formation, in vitro differentiation, cryopreservation, ESC derivation, and many other applications.

Rely on improved performance
KnockOut SR – Multi-Species has a more defined formulation compared to FBS, which contains many undefined and variable components. Unlike most FBS products, each lot is tested for its ability to support PSCs. Because of this, culturing mouse and human PSCs with KnockOut SR – Multi-Species leads to less differentiation and more compact colony morphology compared to FBS.

Enjoy the peace of mind of having stable price and supply
KnockOut SR – Multi-Species is not affected by the limited supply and fluctuating prices often seen with FBS. This makes it easier to plan your budget and buy what you need, especially since it is more affordable than most FBS products.

CTS™ KnockOut™ SR XenoFree Medium (Gibco™)

GIBCO® CTS™ KnockOut™ SR XenoFree Medium, when formulated as a complete medium, enables the growth and expansion of human embryonic stem cells (hESC) and human induced pluripotent stem cells (hiPSC) in a cell culture medium containing only human-derived or human recombinant proteins, to facilitate the transition of hESC and hiPSC research from the bench to the clinic.

The CTS™ product line enables you to reduce your burden in qualifying reagents during your transition from research applications to clinical applications.

• Defined, xenofree medium for hESC⁄iPSC stem cell culture.
• First-to-market xenofree hESC⁄iPSC product intended for Ex Vivo tissue and cell culture processing applications.

CTS™ KnockOut™ SR XenoFree Medium does not contain bovine or other non-human, animal-derived components. Besides pluripotent stem cell culture expansion and maintenance, CTS™ KnockOut™ SR XenoFree Medium can be used for hESC⁄hiPSC cryopreservation, derivation, and differentiation studies.

GIBCO® CTS™ products are of high quality, and are supplied with harmonized documentation such as Certificates of Analysis, Certificates of Origin, and access to authorization letters for our Drug Master Files, as appropriate.

For human ex vivo tissue and cell culture processing applications. CAUTION: Not intended for direct administration to humans or animals.