Shop All Growth Supplements for Primary & Stem Cells

RevitaCell™ Supplement (100X) (Gibco™)

RevitaCell™ Supplement (100X) has been optimized for use with pluripotent stem cells (PSCs), either as a post-thaw recovery solution to improve cell viability or in combination with Essential 8™ Medium to enhance single cell passaging applications. It can also be used for recovery of primary neurons, traditionally a challenging cell to efficiently recover. The significant improvement seen in these applications is due to the presence of a more specific ROCK inhibitor coupled with molecules containing antioxidant and free radical scavenger properties that minimize the impact of stress on the cells.

• Enabling—provides maximum cell viability when performing single cell passaging
• Rapid cell recovery—high post-thaw cell viability and growth of pluripotent stem cells (passaged as clumps or single cells) and neurons
• Consistent—formulated using animal origin-free and chemically defined materials for quality and confidence

Single cell passaging
Use of the RevitaCellTM Supplement for 18-24 hours post-single cell passaging provides vast improvement in cell survival. Cells are shown to reach passaging confluency within 3-4 days post-seeding.

Higher specificity
Kinase profiling of pro-survival small molecules in RevitaCell™ Supplement shows higher specificity in comparison to traditional ROCK inhibitors (i.e., Y-27632 and Thiazovivin).

Quicker post-thaw recovery
PSCs recovered at 40,000 viable cells/cm2 using media supplemented with RevitaCell™ Supplement for the first 24 hours post-thaw recovery followed by feeding with unsupplemented Essential 8™ Medium will typically reach 80% confluency in <5 days, significantly reducing the average post-thaw recovery time of most cryopreservation solutions. Neurite outgrowth of neurons is also significantly improved using RevitaCell™ Supplement.

HuMEC Supplement Kit (Gibco™)

HuMEC Supplement Kit contains growth factors and other components and is designed to work with HuMEC Basal Serum-Free Medium (12753018). Together, these products are engineered specifically for the culture of human mammary epithelial cells (A10565). This translates to greater experimental validity, more reproducible results, and reduction of lot-to-lot variability and aberrations that can occur with the addition of serum. Human mammary epithelial cells cultured in HuMEC Medium have gene expression profiles consistent with normal mammary epithelial cells, as tested on a cDNA microarray.

Applications:
HuMEC Medium is optimized specifically for the growth of human mammary epithelial cells, and has been performance tested in a growth assay using normal human mammary epithelial cells. HuMEC Medium promotes superior growth of normal human mammary epithelial cells, making it ideal for researchers in breast cancer, drug discovery, and other aspects of breast biology.

Contents and Storage:
The HuMEC Supplement Kit includes 5 ml of a supplement mix containing epidermal growth factor, hydrocortisone, isoproterenol, transferrin, and insulin, and 25 mg of bovine pituitary extract. Store the supplement mix at -5°C to -20°C.

Smooth Muscle Growth Supplement (SMGS) (Gibco™)

A sterile, concentrated (20X) solution intended for use with Medium 231 to culture human smooth muscle cells. Each 25 ml bottle of SMGS contains all of the growth factors, hormones, and tissue extracts necessary for the culture of human smooth muscle cells, and is the correct amount of supplement for a 500 ml bottle of basal Medium 231.

When a 500 ml bottle of medium is supplemented with SMGS, the final concentrations of the components in the supplemented medium are:

• Fetal bovine serum (4.9% v/v)
• Human basic fibroblast growth factor (2 ng/ml)
• Human epidermal growth factor (0.5 ng/ml)
• Heparin (5 ng/ml)
• Recombinant human insulin-like growth factor-I (2 µg/ml)
• BSA (0.2 µg/ml)

Large Vessel Endothelial Supplement (LVES) (Gibco™)

Gibco® Large Vessel Endothelial Supplement (LVES) is a 50X, complete, ready-to-use media supplement optimized for use with Gibco® Medium 200 and Medium 200PRF for culture of primary human umbilical vein endothelial cells (HUVEC), human aortic endothelial cells (HAEC), and human pulmonary artery endothelial cells (HPAEC). This blended supplement has been designed to deliver optimal cell growth and to enable superior cell performance for angiogenesis applications, such as cell migration and tube formation. LVES contains fetal bovine serum, hydrocortisone, human epidermal growth factor, basic fibroblast growth factor, heparin, and ascorbic acid.

• Perform tube formation, cell invasion, cell migration, and cell proliferation functional assays
• Achieve superior growth rates of endothelial cells compared to low serum growth supplement (LSGS)
• Expand primary large vessel endothelial cells without genomic alteration

Verified Performance in Key Angiogenesis Applications
Large Vessel Endothelial Supplement is designed to deliver optimal performance in tube formation, cell invasion, cell migration, and cell proliferation assays when used in conjunction with Medium 200, human umbilical vein endothelial cells, and Geltrex® LDEV-Free Reduced Growth Factor Basement Membrane Matrix. These items and a detailed protocol are available in a convenient Angiogenesis Starter Kit or they can be purchased separately.

Maximum Primary Cell Growth with One Blended Supplement
Large Vessel Endothelial Supplement is a complete, ready-to-use blend of growth supplements optimized to deliver>4 x 104 HUVEC per cm2 when used with Medium 200 or Medium 200PRF. Cell phenotype is retained and verified positive for von Willebrand factor (vWf) and CD31, negative for a-actin, and positive for dil-Ac-LDL uptake via ICC.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

Human Keratinocyte Growth Supplement Kit (HKGS Kit) (Gibco™)

A sterile set of solutions intended for use with EpiLife® medium or Medium 154 to culture human epidermal keratinocytes. The HKGS Kit provides, in separate vials, all the components of complete HKGS (S-001-5). A vial of gentamycin/amphotericin solution (GA) is also included. Use of GA is optional.

StemPro™ Neural Supplement (Gibco™)

StemPro Neural Supplement is an optimal, convenient, and cost-effective serum-free supplement for the proliferation of mammalian neural stem and progenitor cells such as human and rat neural stem cells (NSC) and glial progenitor cells (GPC). Due to their scarce availability, the in vitro expansion of these cells has been a challenge to academic and clinical researchers. When combined with the appropriate basal medium and growth factors, StemPro Neural Supplement not only supports the expansion of neuronal progenitor cells but also enables the cells to retain their progenitor status and potential to differentiate into neurons and glial cells.

In recent years, there has been an increased scientific interest in studying neural progenitor cells due to their important role in neurogenesis. Recent advances have also pointed to the potential use of neural progenitor cells as effective therapies for neurodegenerative diseases, such as dopaminergic neural progenitor cells for Parkinson’s Disease and oligoprogenitor cells for amyotrophic lateral sclerosis (ALS) and multiple sclerosis (MS).

For established protocols using StemPro Neural Supplement, access our Gibco Neurobiology Protocol Handbook.

View publications using Gibco neurobiology products at our Gibco CellCite.

Smooth Muscle Differentiation Supplement (SMDS) (Gibco™)

A sterile, concentrated (100X) solution intended for use with Medium 231 to enhance differentiation of human smooth muscle cells. Each 5 ml bottle of SMDS contains the correct amount of differentiation supplement for a 500 ml bottle of Medium 231. When a 500 ml bottle of medium is supplemented with SMDS, the final concentrations of the components in the supplemented medium are:
• fetal bovine serum, 1% v/v
• heparin, 30 µg/ml

Dopaminergic Neuron Maturation Supplement (Gibco™)

Dopaminergic Neuron Maturation Supplement is a component of the Gibco™ PSC Dopaminergic Neuron Differentiation Kit that is also offered separately. It enables maturation of excess floor plate progenitor cells to midbrain dopaminergic neurons.

Human Melanocyte Growth Supplement (HMGS) (Gibco™)

A sterile, concentrated (100X) solution intended for use with Medium 254 or Medium 254CF to culture human epidermal melanocytes. Each 5 ml bottle of HMGS contains all of the growth factors, hormones, and tissue extracts necessary for the culture of human epidermal melanocytes and is the correct amount of supplement for a 500 ml bottle of basal Medium 254 or Medium 254CF.

When a 500 ml bottle of medium is supplemented with HMGS, the final concentrations of the components in the supplemented medium are:

• Bovine pituitary extract (BPE) (0.2% v/v)
• Fetal bovine serum (0.5% v/v)
• Recombinant human insulin-like growth factor-I (1 µg/ml)
• Bovine transferrin (5 µg/ml)
• Basic fibroblast growth factor (3 ng/ml)
• Hydrocortisone (0.18 µg/ml)
• Heparin (3 µg/ml)
• Phorbol 12-myristate 13-acetate (PMA) (10 ng/ml)

Microvascular Growth Supplement (MVGS) (Gibco™)

A sterile, concentrated (20X) solution intended for use with Medium 131 to culture human microvascular endothelial cells. Each 25 ml bottle of MVGS contains all of the growth factors, hormones, and tissue extracts necessary for the culture of human microvascular endothelial cells, and is the correct amount of supplement for a 500 ml bottle of Medium 131. When a 500 ml bottle of medium is supplemented with MVGS, the final concentrations of the components in the supplemented medium are:
• fetal bovine serum (4.9% v/v final concentration)
• hydrocortisone (1 µg/ml)
• human fibroblast growth factor (3 ng/ml)
• heparin (10 µg/ml)
• human epidermal growth factor (1 ng/ml)
• dibutyryl cyclic AMP (0.08 mM)

Human Corneal Growth Supplement (HCGS) (Gibco™)

Human Corneal Growth Supplement (HCGS) is a sterile, concentrated (100×) solution intended for use with EpiLife® medium to culture human corneal epithelial cells. HCGS is an ionically-balanced supplement containing all of the growth factors, hormones, and tissue extracts necessary for the culture of human corneal epithelial cells, and is provided in the correct amount to supplement a 500 ml bottle of medium.

Human Keratinocyte Growth Supplement (HKGS) (Gibco™)

A sterile, concentrated (100X) solution intended for use with EpiLife® medium or Medium 154 to culture human epidermal keratinocytes. HKGS is an ionically-balanced supplement containing bovine pituitary extract (BPE), recombinant human insulin-like growth factor-I, hydrocortisone, bovine transferrin, and human epidermal growth factor. Each 5 mL bottle of HKGS contains all of the growth factors, hormones, and tissue extracts necessary for the culture of human epidermal keratinocytes, and is the correct amount of supplement for a 500 mL bottle of medium.

When a 500 mL bottle of medium is supplemented with HKGS, the final concentrations of the components in the supplemented medium are:

• Bovine pituitary extract (BPE), 0.2% v/v
• Recombinant human insulin-like growth factor-I, 1 µg/mL
• Hydrocortisone, 0.18 µg/mL
• Bovine transferrin, 5 µg/mL
• Human epidermal growth factor, 0.2 ng/mL

Low Serum Growth Supplement Kit (LSGS Kit) (Gibco™)

The LSGS Kit provides, in separate vials, all the components of complete LSGS (S-003-10): fetal bovine serum, hydrocortisone, human epidermal growth factor, and basic fibroblast growth factor/heparin (stabilized with BSA). A vial of gentamycin/amphotericin solution (GA) is also included. Use of GA is optional. When a 500 ml bottle of medium is supplemented with LSGS or the components of the LSGS Kit, the final concentrations of the components in the supplemented medium are:
• fetal bovine serum, 2% v/v
• hydrocortisone (1 µg/ml)
• human epidermal growth factor, 10 ng/ml
• basic fibroblast growth factor, 3 ng/ml
• heparin, 10 µg/ml

Supplement S7 (Gibco™)

Supplement S7 is a sterile, concentrated (100X), ionically balanced solution intended for use as one component in a complete culture environment for human epidermal keratinocytes. Supplement S7 is chemically defined and animal origin-free. Each 5 ml bottle of Supplement S7 is the correct amount of supplement for a 500 ml bottle of EpiLife® basal medium.

Primary Hepatocyte Maintenance Supplements (Gibco™)

Hepatocyte Maintenance Supplement Packs allow you to supplement up to 500 ml of Williams Medium E without phenol red (or suitable alternative basal medium) for the purpose of incubating hepatocytes in suspension or plated cultures.

Hepatocyte Maintenance Supplement Packs contain:
• Dexamethasone
• A cocktail solution of penicillin-streptomycin, ITS+ (insulin, transferrin, selenium complex, BSA, and linoleic acid), GlutaMAX™, and HEPES

Note: Kit components may ship separately due to differences in storage temperatures.

Related links:
Learn more about our cell culture reagents for hepatocytes
Learn more about our cryopreserved hepatocytes
Learn about our full line of hepatocytes