Shop All Growth Supplements for Primary & Stem Cells

Primary Hepatocyte Thawing and Plating Supplements (Gibco™)

Hepatocyte Thawing and Plating Supplement Packs allow you to supplement up to 500 ml of Williams Medium E without phenol red (or a suitable alternative basal medium) for the purpose of plating fresh or cryopreserved hepatocytes.

Hepatocyte Thawing and Plating Supplement Packs contain:
• Prequalified fetal bovine serum
• Dexamethasone
• A cocktail solution of fetal bovine serum, penicillin-streptomycin, human recombinant insulin, GlutaMAX™, and HEPES

Note: Kit components may ship separately due to differences in storage temperatures.

Related Links:
Learn more about our cell culture reagents for hepatocytes
Learn more about our cryopreserved hepatocytes
Learn about our full line of hepatocytes

Primary Hepatocyte Maintenance Supplements (Gibco™)

Hepatocyte Maintenance Supplement Packs allow you to supplement up to 500 ml of Williams Medium E without phenol red (or suitable alternative basal medium) for the purpose of incubating hepatocytes in suspension or plated cultures.

Hepatocyte Maintenance Supplement Packs contain:
• Dexamethasone
• A cocktail solution of penicillin-streptomycin, ITS+ (insulin, transferrin, selenium complex, BSA, and linoleic acid), GlutaMAX™, and HEPES

Note: Kit components may ship separately due to differences in storage temperatures.

Related links:
Learn more about our cell culture reagents for hepatocytes
Learn more about our cryopreserved hepatocytes
Learn about our full line of hepatocytes

Large Vessel Endothelial Supplement (LVES) (Gibco™)

Gibco® Large Vessel Endothelial Supplement (LVES) is a 50X, complete, ready-to-use media supplement optimized for use with Gibco® Medium 200 and Medium 200PRF for culture of primary human umbilical vein endothelial cells (HUVEC), human aortic endothelial cells (HAEC), and human pulmonary artery endothelial cells (HPAEC). This blended supplement has been designed to deliver optimal cell growth and to enable superior cell performance for angiogenesis applications, such as cell migration and tube formation. LVES contains fetal bovine serum, hydrocortisone, human epidermal growth factor, basic fibroblast growth factor, heparin, and ascorbic acid.

• Perform tube formation, cell invasion, cell migration, and cell proliferation functional assays
• Achieve superior growth rates of endothelial cells compared to low serum growth supplement (LSGS)
• Expand primary large vessel endothelial cells without genomic alteration

Verified Performance in Key Angiogenesis Applications
Large Vessel Endothelial Supplement is designed to deliver optimal performance in tube formation, cell invasion, cell migration, and cell proliferation assays when used in conjunction with Medium 200, human umbilical vein endothelial cells, and Geltrex® LDEV-Free Reduced Growth Factor Basement Membrane Matrix. These items and a detailed protocol are available in a convenient Angiogenesis Starter Kit or they can be purchased separately.

Maximum Primary Cell Growth with One Blended Supplement
Large Vessel Endothelial Supplement is a complete, ready-to-use blend of growth supplements optimized to deliver>4 x 104 HUVEC per cm2 when used with Medium 200 or Medium 200PRF. Cell phenotype is retained and verified positive for von Willebrand factor (vWf) and CD31, negative for a-actin, and positive for dil-Ac-LDL uptake via ICC.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

Supplement S7 (Gibco™)

Supplement S7 is a sterile, concentrated (100X), ionically balanced solution intended for use as one component in a complete culture environment for human epidermal keratinocytes. Supplement S7 is chemically defined and animal origin-free. Each 5 ml bottle of Supplement S7 is the correct amount of supplement for a 500 ml bottle of EpiLife® basal medium.

Mammary Epithelial Growth Supplement (MEGS) (Gibco™)

A sterile, concentrated (100X) solution intended for use as one component in a complete culture environment for the growth of human mammary epithelial cells. Each 5 ml bottle of MEGS contains all of the growth factors, hormones, and tissue extracts necessary for the culture of mammary epithelial cells and is the correct amount of supplement for a 500 ml bottle of Medium 171.

When a 500 ml bottle of medium is supplemented with MEGS, the final concentrations of the components in the supplemented medium are:

• Bovine pituitary extract (BPE) (0.4% v/v)
• Recombinant human insulin-like growth factor-I (1 µg/ml)
• Hydrocortisone (0.5 µg/ml)
• Human epidermal growth factor (3 ng/ml)

Smooth Muscle Differentiation Supplement (SMDS) (Gibco™)

A sterile, concentrated (100X) solution intended for use with Medium 231 to enhance differentiation of human smooth muscle cells. Each 5 ml bottle of SMDS contains the correct amount of differentiation supplement for a 500 ml bottle of Medium 231. When a 500 ml bottle of medium is supplemented with SMDS, the final concentrations of the components in the supplemented medium are:
• fetal bovine serum, 1% v/v
• heparin, 30 µg/ml

Low Serum Growth Supplement Kit (LSGS Kit) (Gibco™)

The LSGS Kit provides, in separate vials, all the components of complete LSGS (S-003-10): fetal bovine serum, hydrocortisone, human epidermal growth factor, and basic fibroblast growth factor/heparin (stabilized with BSA). A vial of gentamycin/amphotericin solution (GA) is also included. Use of GA is optional. When a 500 ml bottle of medium is supplemented with LSGS or the components of the LSGS Kit, the final concentrations of the components in the supplemented medium are:
• fetal bovine serum, 2% v/v
• hydrocortisone (1 µg/ml)
• human epidermal growth factor, 10 ng/ml
• basic fibroblast growth factor, 3 ng/ml
• heparin, 10 µg/ml

Human Melanocyte Growth Supplement-2, PMA-free (HMGS-2) (Gibco™)

A sterile, concentrated (100X) solution intended for use with Medium 254 or Medium 254CF to culture human epidermal melanocytes. Each 5ml bottle of HMGS-2 contains all of the growth factors, hormones, and tissue extracts necessary for the culture of human epidermal melanocytes and is the correct amount of supplement for a 500 ml bottle of medium.

When a 500 ml bottle of medium is supplemented with HMGS-2, the final concentrations of the components in the supplemented medium are:

• Fetal bovine serum (0.5%)
• Basic fibroblast growth factor (3 ng/ml)
• Bovine pituitary extract (0.2%)
• Heparin (3 µg/ml)
• Hydrocortisone (0.18 µg/ml)
• Recombinant human insulin-like growth factor-I (1 µg/ml)
• Transferrin (5 µg/ml)
• Endothelin-1 (10 nM)

EpiLife™ Defined Growth Supplement (EDGS) (Gibco™)

A defined, sterile, concentrated (100X) solution intended for use with EpiLife® medium to culture human epidermal keratinocytes. EDGS is not intended for use with Medium 154. EDGS contains all of the growth factors and hormones necessary for the growth of human epidermal keratinocytes. Each bottle contains the correct amount of supplement for a 500 ml bottle of EpiLife® medium. EDGS contains:
• purified bovine serum albumin
• purified bovine transferrin
• hydrocortisone
• recombinant human insulin-like growth factor type-1 (rhIGF-1)
• prostaglandin E2 (PGE2)
• recombinant human epidermal growth factor (rhEGF) The bovine products in EDGS have been isolated from animals of North American origin. The isolation process for these components includes steps that inactivate viruses. All components of EDGS are greater than 95% pure.

Dopaminergic Neuron Maturation Supplement (Gibco™)

Dopaminergic Neuron Maturation Supplement is a component of the Gibco™ PSC Dopaminergic Neuron Differentiation Kit that is also offered separately. It enables maturation of excess floor plate progenitor cells to midbrain dopaminergic neurons.

Smooth Muscle Growth Supplement (SMGS) (Gibco™)

A sterile, concentrated (20X) solution intended for use with Medium 231 to culture human smooth muscle cells. Each 25 ml bottle of SMGS contains all of the growth factors, hormones, and tissue extracts necessary for the culture of human smooth muscle cells, and is the correct amount of supplement for a 500 ml bottle of basal Medium 231.

When a 500 ml bottle of medium is supplemented with SMGS, the final concentrations of the components in the supplemented medium are:

• Fetal bovine serum (4.9% v/v)
• Human basic fibroblast growth factor (2 ng/ml)
• Human epidermal growth factor (0.5 ng/ml)
• Heparin (5 ng/ml)
• Recombinant human insulin-like growth factor-I (2 µg/ml)
• BSA (0.2 µg/ml)

RevitaCell™ Supplement (100X) (Gibco™)

RevitaCell™ Supplement (100X) has been optimized for use with pluripotent stem cells (PSCs), either as a post-thaw recovery solution to improve cell viability or in combination with Essential 8™ Medium to enhance single cell passaging applications. It can also be used for recovery of primary neurons, traditionally a challenging cell to efficiently recover. The significant improvement seen in these applications is due to the presence of a more specific ROCK inhibitor coupled with molecules containing antioxidant and free radical scavenger properties that minimize the impact of stress on the cells.

• Enabling—provides maximum cell viability when performing single cell passaging
• Rapid cell recovery—high post-thaw cell viability and growth of pluripotent stem cells (passaged as clumps or single cells) and neurons
• Consistent—formulated using animal origin-free and chemically defined materials for quality and confidence

Single cell passaging
Use of the RevitaCellTM Supplement for 18-24 hours post-single cell passaging provides vast improvement in cell survival. Cells are shown to reach passaging confluency within 3-4 days post-seeding.

Higher specificity
Kinase profiling of pro-survival small molecules in RevitaCell™ Supplement shows higher specificity in comparison to traditional ROCK inhibitors (i.e., Y-27632 and Thiazovivin).

Quicker post-thaw recovery
PSCs recovered at 40,000 viable cells/cm2 using media supplemented with RevitaCell™ Supplement for the first 24 hours post-thaw recovery followed by feeding with unsupplemented Essential 8™ Medium will typically reach 80% confluency in <5 days, significantly reducing the average post-thaw recovery time of most cryopreservation solutions. Neurite outgrowth of neurons is also significantly improved using RevitaCell™ Supplement.

StemPro™ Neural Supplement (Gibco™)

StemPro Neural Supplement is an optimal, convenient, and cost-effective serum-free supplement for the proliferation of mammalian neural stem and progenitor cells such as human and rat neural stem cells (NSC) and glial progenitor cells (GPC). Due to their scarce availability, the in vitro expansion of these cells has been a challenge to academic and clinical researchers. When combined with the appropriate basal medium and growth factors, StemPro Neural Supplement not only supports the expansion of neuronal progenitor cells but also enables the cells to retain their progenitor status and potential to differentiate into neurons and glial cells.

In recent years, there has been an increased scientific interest in studying neural progenitor cells due to their important role in neurogenesis. Recent advances have also pointed to the potential use of neural progenitor cells as effective therapies for neurodegenerative diseases, such as dopaminergic neural progenitor cells for Parkinson’s Disease and oligoprogenitor cells for amyotrophic lateral sclerosis (ALS) and multiple sclerosis (MS).

For established protocols using StemPro Neural Supplement, access our Gibco Neurobiology Protocol Handbook.

View publications using Gibco neurobiology products at our Gibco CellCite.

Low Serum Growth Supplement (LSGS) (Gibco™)

Low Serum Growth Supplement (LSGS) is a sterile, concentrated (50X) solution intended for use as one component in a complete culture environment for the growth of human dermal fibroblasts and endothelial cells derived from large vessels. LSGS is intended for use with Medium 106 (fibroblasts) or Medium 200 (endothelial cells). LSGS contains: fetal bovine serum, basic fibroblast growth factor, heparin, hydrocortisone, and epidermal growth factor. When a 500 ml bottle of medium is supplemented with LSGS or the components of the LSGS Kit, the final concentrations of the components in the supplemented medium are:
• fetal bovine serum, 2% v/v
• hydrocortisone (1 µg/ml)
• human epidermal growth factor, 10 ng/ml
• basic fibroblast growth factor, 3 ng/ml
• heparin, 10 µg/ml

Human Corneal Growth Supplement (HCGS) (Gibco™)

Human Corneal Growth Supplement (HCGS) is a sterile, concentrated (100×) solution intended for use with EpiLife® medium to culture human corneal epithelial cells. HCGS is an ionically-balanced supplement containing all of the growth factors, hormones, and tissue extracts necessary for the culture of human corneal epithelial cells, and is provided in the correct amount to supplement a 500 ml bottle of medium.