Shop All CHO Media Supplements

Resurge™ CD3 Supplement, without glucose and L-glutamine (Gibco™)

Resurge CD3 Supplement, without glucose and L-glutamine, is an animal origin-free (AOF) protein-free chemically defined supplement. It can be used in batch and fed batch mode in cell culture processes. All components are chemically defined with known molecular formulae and structures and meet our stringent Animal Origin-Free (AOF) specification.

Resurge CD3 Supplement is formulated without glucose, L-glutamine, and sodium bicarbonate. Due to the balanced nutritional nature of this supplement, rich in amino acids, it has also been used in bacterial applications.

Features:
• Animal origin-free
• Chemically defined
• Protein-free
• Rich in amino acids

Applications of the Resurge CD3 Supplement, without glucose and L-glutamine, include:
• Can be used in batch and fed batch mode in cell culture processes
• Use in bacterial applications

GlycanTune™ A+ Total Feed (Gibco™)

GlycanTune™ A+ Total Feed is an AGT™, or Advanced Granulation Technology™, feeding supplement specifically designed for a fed-batch culture of multiple cell lines. When used, this dry-format, single-part total feeding supplement can shift glycan profiles from heavily G0F to mostly G1F and G2F glycans. The product is an animal origin–free, chemically-defined formulation that contains no proteins, hydrolysates, or components of unknown composition. GlycanTune A+ Total Feed provides an easy-to-reconstitute, pH-neutral solution that can be concentrated from the suggested 73.0 g/L (1X) up to 219.0 g/L (3X). As a result, key nutrients can be delivered in smaller volumes.

Which GlycanTune Total Feed to choose?

Consider GlycanTune A+ Total Feed when:
• You have used Gibco™ EfficientFeed™ A+ (Plus) AGT Supplement in the past and wish to maintain the benefits of simpler reconstitution and super-concentration in delivering key components alongside improved glycan profile
• You are currently using another feed and wish to shift glycan profile more toward G1 and G2
• You are using a cell line or base medium where CD OptiCHO™ Medium may be considered an option

Consider GlycanTune B+ Total Feed when:
• You have used Gibco EfficientFeed B+ (Plus) AGT Supplement in the past and wish to maintain the benefits of simpler reconstitution and super-concentration in delivering key components alongside improved glycan profile
• You are currently using another feed and wish to shift glycan profile more toward G1 and G2
• You are using a cell line or base medium where CD CHO Medium may be considered an option

Consider GlycanTune C+ Total Feed when:
• You have used Gibco EfficientFeed C+ (Plus) AGT Supplement in the past and wish to maintain the benefits of simpler reconstitution and super-concentration in delivering key components alongside improved glycan profile
• You are currently using another feed and wish to shift glycan profile more toward G1 and G2
• You are using a cell line or base medium where Dynamis™ Medium may be considered an option

Dialing in a glycan profile
By following the protocol recommended to test with GlycanTune Total Feed, it is possible to shift the glycan protein expression profiles from primarily G0 to primarily G1 and G2.

Increasing titers
As a complete feed, GlycanTune total feeds have been shown to provide multiple-fold increases in titers at 1X concentration versus batch culture; as such, they are an excellent starting point for process development when a shift in glycan profile is desired. Flexibility to further increase titers is enhanced with the ability to super-concentrate the product by reconstituting with less water to deliver up to 219.0 g/L of fed nutrients (see figure).

Maximizing bioreactor utilization
Have you ever wished you could have more working volume at the start of a bioreactor run to get more protein produced, but needed too much feeding volume space to accomplish your optimal goal? Have you ever thought that room for a little more feeding might get you to a new level of titer from a process and maybe even reduce the number of bioreactor runs that might be needed per year? In either of these cases, it is important to have the flexibility to super-concentrate your feeding supplements so you get less product dilution, more space for an additional feeding, and/or more working volume to start your run (see figure). In fact, you may gain about 20% more bioreactor utilization by exploring the unique ability to super-concentrate this GlycanTune A+ product.

Simplified handling to reduce risk in scale-up
The ability to scale up using AGT dry format provides easier handling and storage in manufacturing and other bioprocess settings. Fewer parts and reconstitution steps mean fewer opportunities for operator error. Further, the ability to store this feed, even when super-concentrated, for the length of a bioreactor run at room temperature in the dark may allow less chance of contamination by reducing the number of connections to the bioreactor to one or two—depending on whether GlycanTune Total Feed can be used alone or after a change from your current feed.

GlycanMod™ Pak (Gibco™)

The GlycanMod Pak includes protein quality supplements that provide an innovative solution to your protein quality needs. These chemically defined protein quality supplements are designed to increase mAb galactosylation across multiple cell lines. GlycanMod supplements are available as an easy-to-test four-pack, GlycanMod Pak, or as individual products: GlycanMod G100, G125, G150, and G175 supplements.

Each protein quality supplement has a distinct composition which provides more options for achieving your target profile. These supplements are titratable and can be added as needed to achieve the desired galactosylation pattern. GlycanMod supplements offer a solution for your galactosylation needs, helping to get your product to market faster. GlycanMod Pak offers one each of our GlycanMod supplements in 100-g, non-GMP sizes.

The GlycanMod Pak includes:
• GlycanMod G100—enriched in micronutrients
• GlycanMod G125—enriched in carbohydrates and micronutrients
• GlycanMod G150—enriched in trace elements
• GlycanMod G175—enriched in carbohydrates and trace elements

CD EfficientFeed™ C AGT™ Nutrient Supplement (Gibco™)

GIBCO® CD EfficientFeed™ C AGT™ nutrient supplement is a dry format single part supplement for fed batch addition to the culture of multiple cell lines. This product is designed to work with GS CHO cell lines as a single feed without the need for additional GS supplements. CD EfficientFeed™ C AGT™ nutritient supplement is an animal origin-free, chemically defined formulation that contains no proteins, hydrolysates, or components of incompletely defined composition.

The AGT™ format is designed for large scale manufacturing and ease of use.
• Up to 2.5 – 6 Fold increases versus batch culture – higher concentration of key components
• Higher productivity in GS CHO and other selected cell lines
• Avoid issues associated with the use of hydrolysates and other alternatives while gaining superior performance
• Simplify scale-up by leveraging the value of AGT™ simple reconstitution
• Ready for larger scale use with convenient packaging options (1 L, 10 L, and 100 L)
• No components of animal origin, minimizing the opportunity for introduction of adventitious agents and facilitating the regulatory process

Which EfficientFeed™ Nutrient Supplement to Choose?
Consider CD EfficientFeed™ C AGT™ supplement when:
• You want higher productivity in GS CHO and other selected cell lines
• You are currently using CD FortiCHO™ as your base cell growth medium
• You are using a base medium with greater than 6 g/L glucose or a hydrolysate at day 0

Consider CHO CD EfficientFeed™ A supplement when:
• You are currently using CD OptiCHO™ as your base cell growth medium

Consider CHO CD EfficientFeed™ B supplement when:
• You are currently using CD CHO as your base cell growth medium

Consider the CHO CD Efficient Feed™ Kit when:
• You are using a base medium with less than 6 g/L glucose and no hydrolysate at day 0

Increased Titers
CHO CD EfficientFeed™ supplements have been shown to fold increases in titers versus batch culture, and are an excellent starting point for process development. Furthermore, it does not require addition of a GS Supplement when used in CHO cell lines expressing endogenous glutamine synthetase.

Simple Scale-Up and Cost Savings
The ability to scale up in a dry format provides ease of handling and storage in manufacturing and other bioprocess settings. With AGT™ (Advanced Granulation Technology™) dry format, it is possible to store and use the product even more easily and safely than traditional dry format products. CD EfficientFeed™ C AGT™ supplement is offered in 1 L, 10 L and 100 L packaging that allows scale-up as a seamless process, as well as allow for cost savings when purchasing production level volumes.

AGT™ Format Ease of Use
CD EfficientFeed™ C AGT™ Nutrient Supplement delivers dry format scale-up convenience as single part AGT dry format. For more details, consult the supporting documents provided below.

For research or further manufacturing use. Not for use in diagnostic procedures.

CHO CD EfficientFeed™ B AGT™ Nutrient Supplement (Gibco™)

Gibco® CHO CD EfficientFeed™ B AGT™ nutrient supplement is a single part dry format product for fed batch addition to the culture of multiple cell lines. CHO CD EfficientFeed™ B AGT™ (Advanced Granulation Technology™) nutrient supplement is an animal origin-free, chemically defined formulation that contains no proteins, hydrolysates, or components of incompletely defined composition.

The AGT™ format is designed for large scale manufacturing and ease of use. This product is also available in liquid format for small scale use and experimentation.
• Expect up to 2- to 5-fold increases in productivity
• Avoid issues associated with the use of hydrolysates and other alternatives while gaining superior performance
• Simplify scale-up by leveraging the value of AGT™ simple reconstitution
• Achieve performance comparable to our original liquid catalog product
• Ready for larger scale use with convenient packaging options (10 L and 100 L)

Which EfficientFeed™ Nutrient Supplement to Choose?
Consider the CHO CD Efficient Feed™ Kit when:
• You are using a base medium with less than 6 g/L glucose and no hydrolysate at day 0

Consider CHO CD EfficientFeed™ A supplement when:
• You are currently using CD OptiCHO™ as your base cell growth medium

Consider CHO CD EfficientFeed™ B supplement when:
• You are currently using CD CHO as your base cell growth medium

Consider CD EfficientFeed™ C AGT™ supplement when:
• You want higher productivity in GS CHO and other selected cell lines
• You are currently using CD FortiCHO™ as your base cell growth medium
• You are using a base medium with greater than 6 g/L glucose or a hydrolysate at day 0

Increased Titers
CHO CD EfficientFeed™ supplements have been shown to increase titers 2-5 fold versus batch culture, and are an excellent starting point for process development (Figures 1a & 1b). Furthermore, they outperform competitive alternatives, including hydrolysates (Figures 2, 3a, & 3b).

Simple Scale-Up and Cost Savings
The ability to scale up in a dry format provides ease of handling and storage in manufacturing and other bioprocess settings. With AGT™ (Advanced Granulation Technology™) dry format, it is possible to store and use the product even more easily and safely than traditional dry format products. CHO CD EfficientFeed™ B AGT™ nutrient supplement is offered in 10 L and 100 L packaging that allows scale-up from the 1 L catalog liquid as a seamless process, as well as allow for cost savings when purchasing production level volumes.

Comparable Performance to Liquid Supplement
Equally important as the convenience and simplicity of AGT™ (Advanced Granulation Technology™) dry format is the comparable performance to the liquid CHO CD EfficientFeed™ B (Figure 4). Both forms have performed similarly in our testing, and the AGT™ version has been used successfully as a prototype in many processes by multiple companies.

AGT™ Format Ease of Use
CHO CD EfficientFeed™ B AGT™ nutrient supplement delivers dry format scale-up convenience as well as simple reconstitution via water addition and a single pH adjustment step (see Product Profile Sheet below for complete instructions).

For research or further manufacturing use. Not for use in diagnostic procedures.

GlycanMod™ G150 Supplement (Gibco™)

GlycanMod G150 is a chemically defined protein quality supplement, enriched in trace elements, that is designed to increase mAb galactosylation across multiple cell lines. It is also available as part of the GlycanMod Pak, which includes one of each of our GlycanMod supplements in 100-g, non-GMP sizes.

Each protein quality supplement has a distinct composition which provides more options for achieving your target profile. These supplements are titratable and can be added as needed to achieve the desired galactosylation pattern. GlycanMod supplements offer a solution for your galactosylation needs, helping to get your product to market faster.

The GlycanMod Pak includes:
• GlycanMod G100—enriched in micronutrients
• GlycanMod G125—enriched in carbohydrates and micronutrients
• GlycanMod G150—enriched in trace elements
• GlycanMod G175—enriched in carbohydrates and trace elements

Resurge™ CD4 Supplement, without glucose and L-glutamine (Gibco™)

Resurge CD4 Supplement, without glucose and L-glutamine, is a chemically defined supplement formulation to complement a variety of base media. This supplement was developed using several host cell lines and in various culture systems, including shaker flasks and bench-scale bioreactors. Resurge CD4 Supplement, without glucose and L-glutamine, was developed for use in CHO mammalian culture systems and can be used either as a media supplement or a culture system feed.

EfficientFeed™ B+ AGT™ Supplement (Gibco™)

Gibco® EfficientFeed™ B+ (Plus) AGT™ Supplement is a dry-format, single-part supplement for fed batch addition to the culture of multiple cell lines to assist with process development and maximization of bioreactor utilization. Compared to original CHO CD EfficientFeed™ B AGT™ nutrient supplement, EfficientFeed™ B+ supplement has components that are uniquely combined to allow simpler single-part pH-neutral reconstitution, as well as the ability to super-concentrate the supplement from 52.7 g/L (1X) to 158.1g/L (3X) for reduction of product dilution or increased titers with additional feeding, when appropriate. The product is an animal origin-free, chemically defined formulation that contains no proteins, hydrolysates, or components of incompletely defined composition.

Which EfficientFeed™ + Supplement to Choose?

Consider EfficientFeed™ B+ AGT™ Supplement when:
• You have used CHO CD EfficientFeed™ B supplement in the past and wish to explore simpler reconstitution and super-concentration benefits of new technology for delivery of key components
• You are currently using CD CHO as your base cell growth medium

Consider EfficientFeed™ A+ AGT™ Supplement when:
• You have used CHO CD EfficientFeed™ A supplement in the past and wish to explore simpler reconstitution and super-concentration benefits of new technology for delivery of key components
• You are currently using CD OptiCHO™ as your base cell growth medium

Consider EfficientFeed™ C+ AGT™ Supplement when:
• You have used CD EfficientFeed™ C supplement in the past and wish to explore simpler reconstitution and super-concentration benefits of new technology for delivery of key components
• You want higher productivity in GS CHO, CHO-K1, & CHO-S® derived cell lines
• You are currently using CD FortiCHO™ as your base cell growth medium
• You are using a base medium with greater than 6 g/L glucose or a hydrolysate at day 0

Increasing Titers
As a complete feed, EfficientFeed™ + supplements have been shown to provide multiple-fold increases in titers at 1X concentration versus batch culture, and are an excellent starting point for process development. Flexibility to further increase titers are enhanced with the ability to super-concentrate the product by reconstituting with less water to deliver up to 158 g/L of fed nutrients (see figure).

Maximizing Bioreactor Utilization
Have you ever wished you could have more working volume at the start of a bioreactor run to get more protein produced, but needed too much feeding volume space to accomplish your optimal goal? Have you ever thought that room for a little more feeding might get you to a new level of titer from a process and maybe even reduce the number of bioreactor runs that might be needed per year? In either of these cases, it is important to have the flexibility to super-concentrate your feeding supplements so you get less product dilution, more space for an additional feeding, and/or more working volume to start your run (see figure). In fact, you may gain about 20% more bioreactor utilization by exploring the unique ability to super-concentrate this EfficientFeed B+ product.

Simplified Handling to Reduce Risk in Scale-Up
The ability to scale up in an AGT™ dry format provides ease of handling and storage in manufacturing and other bioprocess settings. Fewer parts and steps in reconstitution mean fewer opportunities for operator error. Further, ability to store this feed, even when super-concentrated, for the length of a bioreactor run at room temperature, in the dark, may allow you fewer chances of contamination during closed system connections by reducing the number of connections to the bioreactor to one.

CHO CD EfficientFeed™ A Nutrient Supplement (Gibco™)

Gibco® CHO CD EfficientFeed™ A liquid nutrient supplement is an animal origin-free, chemically defined complete feed formulation that provides up to 2-5 fold improvement in titers used in fed batch versus batch culture. It contains no proteins, hydrolysates, or components of incompletely defined composition.

The liquid format is designed for small scale use and experimentation. This product is also available in AGT™ format for large scale manufacturing and ease of use.
• Expect up to 2- to 5-fold increases in productivity
• Avoid issues associated with the use of hydrolysates and other alternatives while gaining superior performance
• Ready for larger scale use with convenient packaging options (10 L and 100 L)

Which EfficientFeed™ Nutrient Supplement to Choose?
Consider the CHO CD Efficient Feed™ Kit when:
• You are using a base medium with less than 6 g/L glucose and no hydrolysate at day 0

Consider CHO CD EfficientFeed™ A supplement when:
• You are currently using CD OptiCHO™ as your base cell growth medium

Consider CHO CD EfficientFeed™ B supplement when:
• You are currently using CD CHO as your base cell growth medium

Consider CD EfficientFeed™ C AGT™ supplement when:
• You want higher productivity in GS CHO and other selected cell lines
• You are currently using CD FortiCHO™ as your base cell growth medium
• You are using a base medium with greater than 6 g/L glucose or a hydrolysate at day 0

Increased Titers
CHO CD EfficientFeed™ supplements have been shown to increase titers 2-5 fold versus batch culture, and are an excellent starting point for process development (Figures 1a & 1b). Furthermore, they outperform competitive alternatives, including less consistent hydrolysates (Figures 2, 3a, & 3b).

Simple Scale-Up and Cost Savings
The ability to scale up in a dry format provides ease of handling and storage in manufacturing and other bioprocess settings. With AGT™ (Advanced Granulation Technology™) dry format, it is possible to store and use the product even more easily and safely than traditional dry format products. CHO CD EfficientFeed™ A AGT™ Kit nutrient supplement is offered in 10 L and 100 L packaging that allows scale-up from the 1 L liquid supplement as a seamless process, as well as allow for cost savings when purchasing production level volumes.

Comparable Performance to Liquid Supplement
Equally important as the convenience and simplicity of AGT™ (Advanced Granulation Technology™) dry format is the comparable performance to the liquid CHO CD EfficientFeed™ A supplement (Figure 4). Both forms have performed similarly in our testing, and the AGT™ version has been used successfully as a prototype in many processes by multiple companies.

AGT™ Format Ease of Use
CHO CD EfficientFeed™ A AGT™ Kit Nutrient Supplement delivers dry format scale-up convenience as well as simple reconstitution with approximately 1% concentrated solution add back. For more details, consult the supporting documents provided on the AGT™ format product page.

For research or further manufacturing use. Not for use in diagnostic procedures.

GlycanMod™ G100 Supplement (Gibco™)

GlycanMod G100 is a chemically defined protein quality supplement, enriched in micronutrients, that is designed to increase mAb galactosylation across multiple cell lines. It is also available as part of the GlycanMod Pak, which includes one of each of our GlycanMod supplements in 100-g, non-GMP sizes.

Each protein quality supplement has a distinct composition which provides more options for achieving your target profile. These supplements are titratable and can be added as needed to achieve the desired galactosylation pattern. GlycanMod supplements offer a solution for your galactosylation needs, helping to get your product to market faster.

The GlycanMod Pak includes:
• GlycanMod G100—enriched in micronutrients
• GlycanMod G125—enriched in carbohydrates and micronutrients
• GlycanMod G150—enriched in trace elements
• GlycanMod G175—enriched in carbohydrates and trace elements

Resurge™ CD Pak (Gibco™)

Resurge CD Pak are a diverse set of chemically defined supplement formulations to complement a variety of base media. The CD PAK contains one of each Resurge supplement: CD1, CD2, CD3, CD4, and CD5 at 100 grams each. The supplements have been developed using several host cell lines and in various culture systems, including shaker flasks and bench-scale bioreactors. In order to increase versatility of the formulation, glucose and L-glutamine are not included in the final formulations. Therefore, these two components should be added by users and monitored as appropriate.

Resurge™ CD1 Supplement, without glucose and L-glutamine (Gibco™)

Resurge CD1 Supplement, without glucose and L-glutamine, is a chemically defined supplement formulation to complement a variety of base media. This supplement was developed using several host cell lines and in various culture systems, including shaker flasks and bench-scale bioreactors. Resurge CD1 Supplement, without glucose and L-glutamine, was developed for use in CHO mammalian culture systems and can be used either as a media supplement or a culture system feed.

GlycanTune™ C+ Total Feed (Gibco™)

GlycanTune™ C+ Total Feed is an AGT™, or Advanced Granulation Technology™, feeding supplement specifically designed for a fed-batch culture of multiple cell lines. When used, this dry-format, single-part total feeding supplement can shift glycan profiles from heavily G0F to mostly G1F and G2F glycans. The product is an animal origin–free, chemically-defined formulation that contains no proteins, hydrolysates, or components of unknown composition. GlycanTune C+ Total Feed provides an easy-to-reconstitute, pH-neutral solution that can be concentrated from the suggested 87.7 g/L (1X) up to 175.4 g/L (2X). As a result, key nutrients can be delivered in smaller volumes.

Which GlycanTune Total Feed to choose?

Consider GlycanTune C+ Total Feed when:
• You have used Gibco™ EfficientFeed™ C+ (Plus) AGT Supplement in the past and wish to maintain the benefits of simpler reconstitution and super-concentration in delivering key components alongside improved glycan profile
• You are currently using another feed and wish to shift glycan profile more toward G1 and G2
• You are using a cell line or base medium where Dynamis™ Medium may be considered an option

Consider GlycanTune A+ Total Feed when:
• You have used Gibco EfficientFeed A+ (Plus) AGT Supplement in the past and wish to maintain the benefits of simpler reconstitution and super-concentration in delivering key components alongside improved glycan profile
• You are currently using another feed and wish to shift glycan profile more toward G1 and G2
• You are using a cell line or base medium where CD OptiCHO™ Medium may be considered an option

Consider GlycanTune B+ Total Feed when:
• You have used Gibco EfficientFeed B+ (Plus) AGT Supplement in the past and wish to maintain the benefits of simpler reconstitution and super-concentration in delivering key components alongside improved glycan profile
• You are currently using another feed and wish to shift glycan profile more toward G1 and G2
• You are using a cell line or base medium where CD CHO Medium may be considered an option

Dialing in a glycan profile
By following the protocol recommended to test with GlycanTune Total Feed, it is possible to shift the glycan protein expression profiles from primarily G0 to primarily G1 and G2.

Increasing titers
As a complete feed, GlycanTune total feeds have been shown to provide multiple-fold increases in titers at 1X concentration versus batch culture; as such, they are an excellent starting point for process development when a shift in glycan profile is desired. Flexibility to further increase titers is enhanced with the ability to super-concentrate the product by reconstituting with less water to deliver up to 175.4 g/L of fed nutrients (see figure).

Maximizing bioreactor utilization
Have you ever wished you could have more working volume at the start of a bioreactor run to get more protein produced, but needed too much feeding volume space to accomplish your optimal goal? Have you ever thought that room for a little more feeding might get you to a new level of titer from a process and maybe even reduce the number of bioreactor runs that might be needed per year? In either of these cases, it is important to have the flexibility to super-concentrate your feeding supplements so you get less product dilution, more space for an additional feeding, and/or more working volume to start your run (see figure). In fact, you may gain about 20% more bioreactor utilization by exploring the unique ability to super-concentrate this GlycanTune C+ product.

Simplified handling to reduce risk in scale-up
The ability to scale up using AGT dry format provides easier handling and storage in manufacturing and other bioprocess settings. Fewer parts and reconstitution steps mean fewer opportunities for operator error. Further, the ability to store this feed, even when super-concentrated, for the length of a bioreactor run at room temperature in the dark may allow less chance of contamination by reducing the number of connections to the bioreactor to one or two—depending on whether GlycanTune Total Feed can be used alone or after a change from your current feed.

EfficientFeed™ A+ AGT™ Supplement (Gibco™)

Gibco® EfficientFeed™ A+ (Plus) AGT™ Supplement is a dry-format single-part supplement for fed batch addition to the culture of multiple cell lines to assist with process development and maximization of bioreactor utilization. Compared to original CHO CD EfficientFeed™ A AGT™ nutrient supplement, EfficientFeed™ A+ supplement has components that are uniquely combined to allow simpler single-part pH-neutral reconstitution, as well as the ability to super-concentrate the supplement from 66.4 g/L (1X) to 199.2 g/L (3X) for reduction of product dilution or increased titers with additional feeding, when appropriate. The product is an animal origin-free, chemically defined formulation that contains no proteins, hydrolysates, or components of incompletely defined composition.

Which EfficientFeed™ + Supplement to Choose?

Consider EfficientFeed™ A+ AGT™ Supplement when:
• You have used CHO CD EfficientFeed™ A supplement in the past and wish to explore simpler reconstitution and super-concentration benefits of new technology for delivery of key components
• You are currently using CD OptiCHO™ as your base cell growth medium

Consider EfficientFeed™ B+ AGT™ Supplement when:
• You have used CHO CD EfficientFeed™ B supplement in the past and wish to explore simpler reconstitution and super-concentration benefits of new technology for delivery of key components
• You are currently using CD CHO as your base cell growth medium

Consider EfficientFeed™ C+ AGT™ Supplement when:
• You have used CD EfficientFeed™ C supplement in the past and wish to explore simpler reconstitution and super-concentration benefits of new technology for delivery of key components
• You want higher productivity in GS CHO, CHO-K1, & CHO-S® derived cell lines
• You are currently using CD FortiCHO™ as your base cell growth medium
• You are using a base medium with greater than 6 g/L glucose or a hydrolysate at day 0

Increasing Titers
As a complete feed, EfficientFeed™ + supplements have been shown to provide multiple-fold increases in titers at 1X concentration versus batch culture, and are an excellent starting point for process development. Flexibility to further increase titers are enhanced with the ability to super-concentrate the product by reconstituting with less water to deliver up to 199 g/L of fed nutrients (see figure).

Maximizing Bioreactor Utilization
Have you ever wished you could have more working volume at the start of a bioreactor run to get more protein produced, but needed too much feeding volume space to accomplish your optimal goal? Have you ever thought that room for a little more feeding might get you to a new level of titer from a process and maybe even reduce the number of bioreactor runs that might be needed per year? In either of these cases, it is important to have the flexibility to super-concentrate your feeding supplements so you get less product dilution, more space for an additional feeding, and/or more working volume to start your run (see figure). In fact, you may gain about 20% more bioreactor utilization by exploring the unique ability to super-concentrate this EfficientFeed A+ product.

Simplified Handling to Reduce Risk in Scale-Up
The ability to scale up in an AGT™ dry format provides ease of handling and storage in manufacturing and other bioprocess settings. Fewer parts and steps in reconstitution mean fewer opportunities for operator error. Further, ability to store this feed, even when super-concentrated, for the length of a bioreactor run at room temperature, in the dark, may allow you fewer chances of contamination during closed system connections by reducing the number of connections to the bioreactor to one.

GlycanMod™ G175 Supplement (Gibco™)

GlycanMod G175 is a chemically defined protein quality supplement, enriched in carbohydrates and trace elements, that is designed to increase mAb galactosylation across multiple cell lines. It is also available as part of the GlycanMod Pak, which includes one of each of our GlycanMod supplements in 100-g, non-GMP sizes.

Each protein quality supplement has a distinct composition which provides more options for achieving your target profile. These supplements are titratable and can be added as needed to achieve the desired galactosylation pattern. GlycanMod supplements offer a solution for your galactosylation needs, helping to get your product to market faster.

The GlycanMod Pak includes:
• GlycanMod G100—enriched in micronutrients
• GlycanMod G125—enriched in carbohydrates and micronutrients
• GlycanMod G150—enriched in trace elements
• GlycanMod G175—enriched in carbohydrates and trace elements