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Kanamycin Sulfate Gibco™

Kanamycin Sulfate is a water-soluble antibiotic originally purified from the bacterium Streptomyces kanamyceticus. Kanamycin acts by binding to the 30S subunit of the bacterial ribosome and inhibiting protein synthesis in susceptible bacteria. Gibco® Kanamycin is effective against a wide variety of gram-negative and some gram-positive bacteria, and is used for the prevention of bacterial contamination of cell cultures. We offer a variety of antibiotics and antimycotics for cell culture applications.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

Dual-Site cGMP Manufacturing
For supply chain continuity, we manufacture Gibco® Kanamycin at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.

Gentamicin (10 mg/mL) Gibco™

Gentamicin sulfate is a water-soluble antibiotic drug originally purified from the fungus Micromonospora purpurea. Gentamicin acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition protein synthesis and death in susceptible bacteria. Gibco® Gentamicin is effective against a wide variety of gram-positive and gram-negative bacteria, and is used for the prevention of bacterial contamination on cell cultures. The recommended working concentration ranges from 0.5 to 50 µg/ml. We offer a variety of antibiotics and antimycotics for cell culture applications.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

Dual-Site cGMP Manufacturing
Gibco® Gentamicin is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable Gibco® Gentamicin product made in our Scotland facility (15710-049). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Penicillin-Streptomycin-Glutamine (100X) Gibco™

The antibiotics penicillin and streptomycin are used to prevent bacterial contamination in cell cultures due to their effective combined action against gram-positive and gram-negative bacteria. Penicillin was originally purified from the fungi Penicillium and acts by interfering directly with the turnover of the bacteria cell wall and indirectly by triggering the release of enzymes that further alter the cell wall. Streptomycin was originally purified from Streptomyces griseus . It acts by binding to the 30S subunit of the bacterial ribosome, leading to the inhibition of protein synthesis and death in susceptible bacteria. This solution contains 10,000 units of penicillin, 10,000 µg of streptomycin, and 29.2 mg/ml of L-glutamine in a 10 mM citrate buffer (for pH stability).

We offer a wide range of antibiotics and antimycotics in both powder and liquid formats. See the complete list, or find products for:
Contamination control
Eukaryotic and bacterial selection

See recommended working concentrations for selection antibiotics.

Learn more about the use of antibiotics and antimycotics in cell culture and review guidelines for decontaminating cultures.

Gentamicin (50 mg/mL) Gibco™

Gentamicin sulfate is a water-soluble antibiotic originally purified from the fungus Micromonospora purpurea. Gentamicin acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition of protein synthesis and death in susceptible bacteria. Gibco® Gentamicin is effective against a wide variety of gram-positive and gram-negative bacteria, and is used to prevent the contamination of cell cultures by bacteria. The recommended working concentration ranges from 0.5 to 50 µg /mL.

We offer a wide range of antibiotics and antimycotics in both powder and liquid formats.

Learn more about the use of antibiotics and antimycotics in cell culture and review guidelines for decontaminating cultures.

Dual-Site cGMP Manufacturing
Gibco® Gentamicin is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable Gibco® Gentamicin product made in our Scotland facility (15750-037). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Penicillin-Streptomycin (10,000 U/mL) Gibco™

This solution contains 10,000 units/mL of penicillin and 10,000 µg/mL of streptomycin. The antibiotics penicillin and streptomycin are used to prevent bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria. Penicillin was originally purified from the fungi Penicillium and acts by interfering directly with the turnover of the bacterial cell wall and indirectly by triggering the release of enzymes that further alter the cell wall. Streptomycin was originally purified from Streptomyces griseus. It acts by binding to the 30S subunit of the bacterial ribosome, leading to inhibition of protein synthesis and death in susceptible bacteria.

We offer a wide range of antibiotics and antimycotics in both powder and liquid formats. Learn more about the following types of products:

Cell Culture Antibiotics
Selection Antibiotics (including recommended working concentrations)

Learn more about the use of antibiotics and antimycotics in cell culture and review guidelines for decontaminating cultures.

Actinomycin D Gibco™

Actinomycin D is a nonfluorescent DNA intercalator that exhibits high GC selectivity and causes distortion at its binding site. Binding of the nonfluorescent actinomycin D to nucleic acids changes the absorbance of the dye. Actinomycin D has been used for chromosome banding studies.

Penicillin-Streptomycin-Neomycin (PSN) Antibiotic Mixture Gibco™

The antibiotics Penicillin, Streptomycin, and Neomycin are used to prevent bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria. Penicillin was originally purified from the fungus Penicillium and acts by interfering directly with the turnover of the bacteria cell wall and indirectly by triggering the release of enzymes that further alter the cell wall. Streptomycin was originally purified from Streptomyces griseus and Neomycin from Streptomyces fradiae. Streptomycin and Neomycin act by binding to the 30S subunit of the bacterial ribosome leading to inhibition of protein synthesis and death in susceptible bacteria. We offer a variety of antibiotics and antimycotics for cell culture applications.

This solution contains 5 mg of penicillin, 5 mg of streptomycin, and 10 mg of neomycin per ml in 0.85% saline.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

Geneticin™ Selective Antibiotic (G418 Sulfate), Powder Gibco™

Geneticin® Selective Antibiotic (G418 Sulfate) is produced by the bacterium Micromonospora rhodorangea and acts by binding the ribosome, thus inhibiting protein synthesis in both prokaryotic and eukaryotic cells. Resistance to Geneticin® Selective Antibiotic (G418 Sulfate) is conferred by the E. coli APH (3’) – I and APH (3’) – II resistance genes. Gibco® Geneticin® Selective Antibiotic (G418 Sulfate) is used as a selective antibiotic in the concentration range of 100 - 200 µg/ml for bacteria, or 200 – 500 µg/ml for most mammalian cells. This product is supplied as a powder and should be made into a stock solution of 10 – 50 mg/ml in water. We offer a variety of cell culture antibiotics for your convenience.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

Dual site cGMP Manufacturing and Quality System
For supply chain continuity, we manufacture Gibco® Geneticin® Selective Antibiotic (G418 Sulfate) at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.

Gentamicin/Amphotericin Solution Gibco™

A sterile, concentrated (500×) antibiotic⁄antimycotic solution of gentamicin and amphotericin B (10 × 1 ml vials⁄pkg). One Gentamicin⁄Amphotericin Solution 10-Pak is enough to supplement ten 500 ml bottles of basal medium. Animal origin-free.

Antibiotic-Antimycotic (100X) Gibco™

Gibco® Antibiotic-Antimycotic is used to prevent bacterial and fungal contamination. This solution contains 10,000 units/mL of penicillin, 10,000 µg/mL of streptomycin, and 25 µg/mL of Gibco Amphotericin B. The antibiotics penicillin and streptomycin prevent bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria. Amphotericin B prevents fungal contamination of cell cultures due to its inhibition of multi-cellular fungus and yeast.

Penicillin was originally purified from the fungus Penicillium and acts by interfering directly with the turnover of the bacteria cell wall and indirectly by triggering the release of enzymes that further alter the cell wall. Streptomycin was originally purified from Streptomyces griseus. It acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition of protein synthesis and death in susceptible bacteria. Amphotericin B is an antifungal agent that prevents the growth of fungi and yeast by causing an increase in fungal plasma membrane permeability.

Ampicillin, sodium salt, irradiated Gibco™

Ampicillin is an antibiotic in the broad-spectrum penicillin group. Ampicillin differs from penicillin only by the presence of an amino group, which facilitates penetration through the outer membrane of some gram-negative bacteria. Ampicillin acts by interfering directly with the turnover of the bacterial cell wall and indirectly by triggering the release of enzymes that further alter the cell wall.

Gibco Ampicillin is used as a selective antibiotic generally at a concentration of 20–125 µg/mL. Ampicillin selection is based on hydrolysis and inactivation of its beta-lactam ring by beta-lactamase expressed by the bla gene. Sometimes, beta-lactamase produced by the bacteria is secreted into the medium resulting in inactivation of ampicillin in the culture medium. Ampicillin degradation on agar plates can lead to the formation of satellite colonies. Growth of these colonies can be halted by adding fresh ampicillin. To avoid beta-lactamase buildup, special care must be taken while handling cultures and determining the correct dose of antibiotic.

Ampicillin antibiotic in powder form is an economical choice that can be used in a wide range of gram-positive and gram-negative infections. This product is provided as a powder and should be made into a stock solution at a concentration of 10 mg/mL in water.

cGMP manufacturing
Gibco Ampicillin is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Mycophenolic Acid Gibco™

Gibco® Mycophenolic Acid (MPA) is produced by Penicillium brevi-compactum, Penicillium loniferum and related species. Gibco® Mycophenolic Acid blocks the guanosine monophosphate (GMP) synthesis pathway. Gibco® Mycophenolic Acid is used as a selective agent in mammalian systems, commonly at a concentration of 25 µg/ml. We offer a variety of antibiotics and antimycotics for cell culture applications.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

Carbenicillin, Disodium Salt Gibco™

Carbenicillin Disodium Salt is a semi-synthetic penicillin antibiotic which interferes with cell wall synthesis of gram-negative bacteria while displaying low toxicity to plant tissues. Gibco® Carbenicillin is used as a selective antibiotic for resistant Agrobacterium and E. coli, generally at a concentration of 100 - 500 µg / ml. This product is provided as a powder and should be made into a stock solution at 100 – 500 mg / ml in water. We also offer a variety of cell culture antibiotics.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

Blasticidin S HCl (10 mg/mL) Gibco™

Blasticidin S is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes. It is a potent inhibitor of protein synthesis in in bacteria and eukaryotes, while also active against fungi, nematodes, and tumor cells. Blasticidin S acts by blocking hydrolysis of peptidyl-tRNA induced by release factors and inhibits peptide bond formation. It is used as a selection agent in both mammalian cells and bacterial cells. The recommended working concentration ranges from 1 to 30 µg/mL depending on the cell line and 25–100 µg/mL for bacterial selection. Cell death occurs rapidly, and blasticidin-resistant stable mammalian cell lines can be generated in less than one week.

Resistance to blasticidin S is conferred by BSR and BSD, isolated from Bacillus cereus K55-S and Aspergillus terreus respectively. The BSR resistance gene encodes blasticidin S deaminase, which catalyzes the conversion of blasticidin S to deaminohydroxyblasticidin S. Deaminohydroxyblasticidin S is a biologically inactive derivative of blasticidin S and does not interact with or inhibit prokaryotic or eukaryotic ribosomes. The BSD resistance gene also encodes a blasticidin S deaminase, which catalyzes a similar reaction to the BSR deaminase. For bacterial selection purposes, the salt content of the LB medium must remain low (<90 mM) and the pH should not exceed 7.0 to maintain the activity of basticidin S. A kill curve is recommended in order to determine the minimum effective blasticidin S concentration to kill non-resistant cells.

Applications
View detailed protocols on blasticidin selection in mammalian cells, E. coli, and yeast.

Puromycin Dihydrochloride Gibco™

Puromycin Dihydrochloride is an aminonucleoside antibiotic produced by Streptomyces alboniger. Puromycin works by inhibiting peptidyl transfer on both prokaryotic and eukaryotic ribosomes; resistance is conferred by the expression of the pac gene.

Puromycin is widely used in cell biology as a selection antibiotic agent in mammalian cell culture systems. The recommended working concentration ranges from 0.2–5.0 µg/mL, although it can be toxic to eukaryotic cells at concentrations as low as 1 µg/mL. Gibco® Puromycin Dihydrochloride is supplied at 10 mg/mL in 20 mM HEPES buffer (pH 6.2–6.8) in 10 vials each containing 1 mL.

Other Choices and More Information
We offer a wide range of antibiotics and antimycotics in both powder and liquid formats.

See the complete list, or find products for:
Contamination control
Eukaryotic and bacterial selection

See recommendations for working concentrations for selection antibiotics.

Learn more about the use of antibiotics and antimycotics in cell culture, and review guidelines for decontaminating cultures.
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