Shop All Serum-Free & Specialty Media

PSC Cardiomyocyte Differentiation Kit (Gibco™)

The Gibco® PSC Cardiomyocyte Differentiation Kit consists of a set of serum-free and xeno-free media that enable efficient differentiation of human pluripotent stem cells (PSCs) to contracting cardiomyocytes in as few as 8 days. Unlike other methods that require multiple components and longer assay duration, the PSC Cardiomyocyte Differentiation Kit can generate cardiomyocytes from pluripotent stem cells in a ready-to-use media format and in less time.

Comprised of three 1X media that require no thawing or mixing, each medium is used consecutively over a total of 14 days, resulting in functional cardiomyocytes that express relevant physiological markers, contract in culture, and can be subsequently maintained in culture for >15 days (maintenance medium also sold separately (Cat. No. A2920801)).

No thawing or mixing required
The PSC Cardiomyocyte Differentiation Kit is comprised of 1X media that do not require frozen storage, so no thawing or mixing of reagents. Simply warm the media and add to PSC culture.

Rapid production of cardiomyocytes
The PSC Cardiomyocyte Differentiation Kit is capable of producing contracting cardiomyocytes in as few as 8 days. Differentiated cardiomyocytes can be subsequently maintained in culture for >15 days.

Generation of high quality cardiomyocytes
Cardiomyocytes generated using the PSC Cardiomyocyte Differentiation Kit are functionally relevant, expressing key markers such as TNNT2, Nkx2.5, MYH6, and α-actinin; and contract in culture.

HuMEC Basal Serum-Free Medium (1X) (Gibco™)

HuMEC Basal Serum-Free Medium is designed to work with HuMEC Supplement Kit (SKU# 12755013). Together, these products are engineered specifically for the culture of human mammary epithelial cells (SKU# A10565). This translates to greater experimental validity, more reproducible results, and reduction of lot-to-lot variability and aberrations that can occur with the addition of serum. Human mammary epithelial cells cultured in HuMEC Medium have gene expression profiles consistent with normal mammary epithelial cells, as tested on a cDNA microarray.

Applications:
HuMEC Medium is optimized specifically for the growth of human mammary epithelial cells, and has been performance tested in a growth assay using normal human mammary epithelial cells. HuMEC Medium promotes superior growth of normal human mammary epithelial cells, making it ideal for researchers in breast cancer, drug discovery, and other aspects of breast biology.

Contents and Storage:
HuMEC Basal Serum Free Medium should be supplemented with HuMEC Supplement Kit. HuMEC Supplement Kit and HuMEC Basal Serum Free Media can be ordered together as HuMEC Ready Medium (SKU# 12752010). The HuMEC Supplement Kit includes 5 ml of a supplement mix containing epidermal growth factor, hydrocortisone, isoproterenol, transferrin, and insulin, and 25 mg of bovine pituitary extract. Store the basal medium at +2°C to +8°C.

Medium 200PRF (Gibco™)

A sterile, liquid medium for the culture of human large vessel endothelial cells. Medium 200PRF is Medium 200 prepared without phenol red. This basal medium requires the addition of LSGS (S-003-10) or LSGS Kit (S-003-K) prior to use.

CTS™ OpTmizer™ T Cell Expansion SFM, bag format (Gibco™)

Gibco® OpTmizer™ CTS™ T-Cell Expansion SFM has been developed for the growth and expansion of human T lymphocytes. OpTmizer™ CTS™ T-Cell Expansion medium is a complete serum-free and xeno-free 1X medium consisting of OpTmizer™ T-Cell Expansion Basal Medium (1 L bag) and OpTmizer™ T-Cell Expansion Supplement (26 mL), which are mixed together prior to use.

• Maintains similar phenotype and function as T-cells cultured in conventional serum supplemented medium
• Consistent performance in supporting T-cell expansion
• Supports high density cultures in static and WAVE Bioreactor® cultures
• Be ready for clinical research with this cGmP manufactured formula and support documentation

Versatile T Cell Culture Media
OpTmizer™ CTS™ T-Cell Expansion SFM is designed to give results equivalent to conventional serum-supplemented media. However, if desired, 2% heat-inactivated human serum (Cat. No. 34005-100) may be added to the medium to enhance viability and expansion. The use of serum, and the amount required, should be determined empirically depending on the specific T-cell culture application.

Ready for T Cell Expansion
OpTmizer™ CTS™ T-Cell Expansion SFM is designed for the expansion of human T-cells permitting CD3+ densities of >3x106 cells/mL in static conditions and >2x107 cells/mL in WAVE bags. Results may vary depending on the T-cell subset (e.g., Ag-specific cells). Conditions for specific T-cell culture applications should be determined empirically.

Stability of Complete Media
Complete OpTmizer™ CTS™ T-Cell Expansion SFM (basal, supplement, and L-glutamine) is stable for 4 weeks when stored in the dark at 2–8°C.

Simplify Your Transition into Clinical Research
The Gibco® Cell Therapy Systems (CTS™) brand offers a broad array of products for use in cell therapy applications, including media, reagents, growth factors, enzymes, cell selection/expansion reagents, and devices. Gibco® CTS™ products deliver:
• Reduced burden in qualifying reagents
• Appropriate certificates of analyses and origin included
• Convenient access to our Drug Master File (DMF)

293 SFM II (Gibco™)

293 SFM II is a serum-free, low-protein (<10 µg/ml) formulation optimized for high-density suspension culture of 293 cells (1). (It is not suitable for adherent 293 cell cultures.) This product replaces 293 SFM (Cat. No. 10970-010). 293 SFM II is animal-origin free. Formulated without L-glutamine for increased stability; add 20 ml of 200 mM L-glutamine or 20 ml of GlutaMAX™-I Supplement per 1,000 ml of medium prior to use.

Note:
A 293 Medium Master file has been submitted to the FDA. Permission to cross-reference the Master File may be obtained by contacting Technical Services or your local Sales Representative.

VP-SFM (1X) (Gibco™)

VP-SFM is a serum-free, ultra-low protein (5 µg/ml) medium containing no proteins, peptides, or other components of animal or human origin. It is designed for the growth of VERO cells for virus production (1). This medium is suitable for the growth of COS-7, MDCK, BHK-21 (suspension culture) and HEp2 cells, and for recombinant protein production.

Formulated without L-glutamine for increased stability; add 20 ml of 200 mM L-glutamine or 20 ml of GlutaMAX™-I supplement per 1,000 ml of medium prior to use.

MarrowMAX™ Bone Marrow Medium (Gibco™)

Gibco® MarrowMAX™ Bone Marrow Medium is a fully-supplemented medium developed for the short term culture of bone marrow and other hematopoietic cells for cytogenetic studies and in vitro diagnostic procedures.

Gibco® MarrowMAX™ Bone Marrow Medium features:

• Superior performance
• Ready-to-use format
• Quality and performance testing
• Unique, optimized formulation

Superior performance
Gibco® MarrowMAX™ Bone Marrow Medium outperforms other commercially available media containing Giant Cell Tumor (GCT) conditioned media, providing consistent performance with a higher mitotic index and superior chromosomal morphology (learn more about the superior performance of MarrowMAX™ medium here).

Ready-to-use format
Gibco® MarrowMAX™ Bone Marrow Medium is a frozen, 1X medium, ready to use upon thawing. No supplementation is required. Thawed medium can be stored at 2–8°C for up to 60 days.

Quality and performance testing
Every lot of Gibco® MarrowMAX™ Bone Marrow Medium is performance tested by a certified US reference cytogenetics laboratory to ensure consistently superior performance. Bone marrow cells from the aspirate of a normal adult donor are cultured for 24 hours in Gibco® MarrowMAX™ Bone Marrow Medium before measuring the mitotic index and chromosome banding resolution. In addition, each lot is tested for sterility, pH, osmolality, mycoplasma and endotoxin.

Unique, optimized formulation
Gibco® MarrowMAX™ Bone Marrow Medium is an optimized RPMI 1640 medium, supplemented with Fetal Bovine Serum (FBS), gentamicin, and L-glutamine. It also contains a novel human stromal cell conditioned medium that consists of a unique blend of hematopoietic growth factors. Gibco® MarrowMAX™ Bone Marrow Medium does not contain GCT conditioned medium.

Product Use
For in vitro diagnostic use.

cGMP Manufacturing and Quality System
Gibco® MarrowMAX™ Bone Marrow Medium is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

ExpiCHO™ Expression Medium (Gibco™)

ExpiCHO™ Expression Medium is a GMP-manufactured, chemically-defined, serum-free, protein-free, animal origin–free medium specifically designed for high-density growth and transfection of suspension-adapted Chinese hamster ovary (CHO) cells. It is a core component of the ExpiCHO™ Expression System Kit (Cat. No. A29133) for scalable transient protein production.

ExpiCHO Expression Medium:
• Supports growth of suspension CHO cultures to densities of over 2 x 107 cells per mL, with >95% cell viability
• Uses a transfection-compatible, serum-free suspension culture medium that enables high transfection efficiencies of >90% with ExpiFectamine™ CHO transfection reagent
• Supports transient transfection of suspension CHO cells at a density of 6 x 106 cells per mL
• Facilitates sustained, high-level expression of high-density transiently transfected cultures, achieving yields of up to 3 g per L of recombinant protein
• Supports growth and transfection of CHO cells in culture formats from <1 mL in multi-well plates to >10 L in benchtop bioreactors
• Does not contain phenol red

ExpiCHO Expression Medium is formulated with GlutaMAX™-I reagent. It is ready to use, with no supplementation required. The chemically-defined formulation results in high reproducibility and lot-to-lot reliability. For highest level transient protein expression, the medium is used in combination with a matched culture feed, ExpiCHO™ Feed, which is supplied as part of the ExpiFectamine™ CHO Transfection Kit (Cat. No. A29129). ExpiCHO Expression Medium is not recommended for adherent cell cultures. Cells are typically grown in Erlenmeyer-style flasks on a shaker platform in a humidified, CO2 cell culture incubator. However, cultures are scalable from volumes of <1 mL in multi-well plates to >10 L in bioreactors.

iPSC Lab Starter Kit with Reprogramming (Gibco™)

The Gibco™ iPSC Lab Starter Kit with Reprogramming provides researchers initiating pluripotent stem cell research with the required reagents at a significant discount over purchasing them separately. This package solution is appropriate for labs that plan to perform reprogramming of donor-derived fibroblasts . For labs that are starting from cryopreserved pluripotent stem cells, please see the Gibco™ iPSC Lab Starter Kit, which does not include reprogramming reagents.

The iPSC Lab Starter Kit with Reprogramming includes the following products:

Reprogramming:
• CytoTune-iPS 2.0 Sendai Reprogramming Kit, Cat. No. A16517, 1 kit
• High glucose DMEM with GlutaMAX™-I, Cat. No. 10569-044, 40 x 500 mL
• Gibco™ Mouse Embryonic Fibroblasts (Irradiated), Cat. No. A24903, 11 vials
• MEM Non-Essential Amino Acids Solution (100X), Cat. No. 11140-050, 100 mL
• Fetal Bovine Serum, ES Cell-Qualified, Cat. No. 16141-061, 2 x 100 mL
• Attachment Factor, Cat. No. S-006-100, 2 x 100 mL
• 2-Mercaptoethanol (55 mM), Cat. No. 21985-023, 50 mL
• DMEM/F-12 with GlutaMAX™, Cat. No. 10565-018, 5 x 500 mL
• KnockOut™ Serum Replacement, Cat. No. 10828-010, 3 x 100 mL
• FGF-basic (AA 1-155) Recombinant Human Protein, Cat. No. PHG0264, 4 vials
• Collagenase Type IV, Cat. No. 17104-019, 1 vial
• Tra-1-60 AlexaFluor™ 488 Conjugate for Live Staining, Cat. No. A25618, 1 kit

PSC feeder-free transition and expansion:
• Essential 8™ Adaptation Kit, Cat. No. A25953, 1 kit
• Essential 8™ Medium, Cat. No. A1517001, 4 x 500 mL
• Geltrex™ hESC-qualified Basement Membrane Extract, Cat. No. A1413302, 2 x 5 mL
• Vitronectin (VTN-N), Cat. No. A14700, 3 x 1 mL
• Versene Solution, Cat. No. 15040066, 100 mL
• Dulbecco’s Phosphate Buffered Saline without Ca2+/Mg2+, Cat. No 14190250, 20 x 500 mL

CTS™ AIM V™ SFM (Gibco™)

GIBCO® AIM V® Medium CTS™ (Therapeutic Grade) is the first commercially available defined, serum-free formulation for proliferation and/or manipulation of T cells and dendritic cells and manufactured in compliance with cGMP. AIM V® Medium CTS™ is an FDA 510(k) cleared device which is intended for human ex-vivo tissue & cell culture processing applications. It contains L-glutamine, 50 µg/ml streptomycin sulfate, and 10 µg/ml gentamicin sulfate.

Grace's Insect Medium, supplemented (Gibco™)

Grace's Insect Medium, Supplemented, was originally designed for the growth of cells from the Australian Emperor Gum Moth, Antherea eucalypti. Grace's Supplemented Insect Medium is now widely used for the growth of Spodeptera frugiperda cells, Sf9 and Sf21.

Sf9 and Sf21 cells are routinely used to express recombinant protein using the Baculovirus Expression Vector System (BEVS). We offer a variety of Gibco® media options for a range of insect cell culture applications.

Formulation
This Grace's Insect Medium contains lactalbumin hydrolysate, yeastolate, L-glutamine, and sodium bicarbonate. The complete formulation is available. Grace's Supplemented Insect Medium contains lactalbumin hydrolysate and yeastolate not commonly found in the unsupplemented version.

Dual site cGMP manufacturing and quality system
Gibco® Grace's Supplemented Insect Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical product made in our Scotland facility (11605-045). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Grace's Supplemented Insect Medium contains lactalbumin hydrolysate and yeastolate, and is commonly supplemented with 10% Fetal Bovine Serum (FBS) before use. Grace's Supplemented Insect Medium requires ambient CO2 in order to maintain physiological pH.

CTS™ LV-MAX™ Production Medium

CTS LV-MAX Production Medium is a chemically-defined, serum-free, protein-free medium validated for recombinant lentiviral vector production. In addition to supporting high cell density suspension cultures of CTS Viral Production Cells (HEK293F-derived cells), this medium is suitable for preclinical-, clinical-, and commercial-scale transient transfection and lentiviral production when used with the CTS LV-MAX Transfection Kit. CTS LV-MAX Production Medium undergoes stringent QC testing to ensure it is appropriate for the manufacturing of cell and gene therapy applications.

CTS LV-MAX Production Medium may be applicable for other recombinant viral vector production that uses a HEK293 medium.

CTS LV-MAX Production Medium features include:
• Supports CTS Viral Production Cells to achieve densities of over 1 x 107 cells/mL with minimal cell clumping
• Enables high viral titers of up to 1 x 108 TU/mL (unconcentrated lentiviral vectors–GFP)
• Scalable protocol for 96-deep well blocks, flasks, and bioreactors
• Regulatory-compliant medium manufactured in conformity with cGMP for medical devices, 21 CFR, Part 820, and certified to the ISO 13485. Drug Master File and Regulatory Support File available upon request.

CTS LV-MAX Production Medium is formulated with GlutaMAX-I reagent. It is ready to use, with no supplementation required. The chemically defined formulation results in high reproducibility and lot-to-lot reliability. The medium contains no human or animal-origin components. It is not recommended for adherent cell cultures. The formulation of this product is identical to research-grade LV-MAX Production Medium, but CTS LV-MAX Production Medium is validated for clinical and commercial therapeutic applications.

Custom configuration and packaging available upon request.

FreeStyle™ F17 Expression Medium (Gibco™)

FreeStyle™ F17 Expression Medium is an animal origin–free, chemically defined, protein-free medium specifically developed to support the growth and transfection of 293-F and CHO cells in suspension without adaptation. This is a multi-platform medium compatible with a range of transient expression systems. The addition of 4–8 mM L-glutamine or GlutaMAX™ supplement is required before use. Gibco® FreeStyle™ F17 Expression Medium features:

• Little or no required adaptation
• Ability to support suspension growth and transfection of 293-F and CHO cells
• Animal origin–free, protein-free, and chemically defined formulation
• Scalability in spinner flasks and bioreactors

Little or no required adaptation
Gibco® 293-F, 293-H, FreeStyle™ 293-F, and CHO-S cells in suspension can be cultured directly into Gibco® FreeStyle™ F17 Medium without adaptation. Cells adapted to other commercially available serum-free media can be subcultured directly into Gibco® FreeStyle™ F17 Medium, usually without any further adaptation. Cells usually require adaptation from serum-containing formulations.

Ability to support suspension growth and transfection of 293-F and CHO cells
Gibco® FreeStyle™ F17 Medium provides equal or better performance compared to Gibco® FreeStyle™ 293 Expression Medium and Gibco® FreeStyle™ CHO Medium. Gibco® FreeStyle™ F17 Medium supports 293-F cells at a peak density of 6–7 × 106 viable cells per mL, and supports CHO-S cells at a peak density of 8–9 × 106 viable cells per mL. Cells cultured in Gibco® FreeStyle™ F17 can be transfected using a wide variety of transfection reagents, including lipid-based transfection reagents, such as 293fectin™ and Freestyle™ MAX Reagent. Protein expression typically exceeds 75–150 µg/mL IgG in 293-F cells.

Animal origin–free, protein-free, and chemically defined formulation
Gibco® FreeStyle™ F17 Medium is animal origin–free, protein-free, and chemically defined, allowing for easier purification of your protein of interest. Gibco® chemically defined media contain no proteins, hydrolysates, or components of unknown composition.

Scalability in spinner flasks and bioreactors
Protein production using Gibco® FreeStyle™ F17 Medium can be scaled up in spinner flasks or bioreactors. The appropriate spinner or impeller speed and seeding density should be optimized for each system. Depending on the impeller design and speed, it may be necessary to supplement Gibco® FreeStyle™ F17 Medium with additional Pluronic® F-68 to avoid sheer stress in the culture.

cGMP manufacturing and quality system
Gibco® FreeStyle™ F17 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Dynamis™ AGT™ Medium (Gibco™)

Gibco® Dynamis™ AGT™ dry format medium is specifically designed to offer the highest batch and fed-batch culture performance and yield with recombinant CHO cells in a chemically defined environment. The chemically defined, protein-free, animal origin component-free formulation provides the power to achieve high titers, start process development faster, and streamline or simplify transfer to manufacturing scale.

Power to achieve higher titers
Dynamis™ AGT™ Medium is fortified to help maximize recombinant CHO cell growth and protein expression in suspension batch culture, offering 170% greater production than select competitive media with simple glucose supplementation by day 14. Cultures grown in Dynamis™ AGT™ Medium extend to even higher titers demonstrating good cell viability out to day 21. This medium can also provide up to 60% greater fed-batch growth and production over glucose-only fed-batch culture for selected cell lines and applications when used with EfficientFeed™ C+ AGT™ Supplement.

Power to start process development faster
As a fortified medium, Dynamis™ AGT™ provides the power to get to a high cell density over 10 million cells per mL faster than competitive products and sustains that cell density longer by just maintaining glucose levels. The viable cell growth achieved make it a good choice for scale-up if you have started with CD FortiCHO™ medium. When paired with EfficientFeed™ C+ AGT™ Supplement, it provides a fast and efficient way to develop a process.

The power to streamline transfer to manufacturing
By choosing Dynamis™ AGT™ Medium, you obtain not only the benefits of the medium formulation, but also the benefits of AGT™ dry format technology. This all-in-one product is designed to simplify scale-up and manufacturing by being easy to reconstitute (just add water), with no adjustments of pH or osmolarity necessary. This can help provide efficiencies through supply chain simplicity, reconstitution simplicity, and therefore help reduce expense.

Use Dynamis™ AGT™ Medium when:
• The cell line you are using is a transfected CHO K1, GS CHO, or CHO-S™ cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processing

When to consider another Gibco® medium

Consider CD OptiCHO™ Medium when:
• You are using a transfected CHO cell line other than CHO K1, GS CHO, or CHO-S™ cell lines
• Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
• Your CHO cell line is hard to grow or adapt

Consider CD CHO Medium when:
• Your GS CHO cells are recommended to be grown in CD CHO medium
• Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)

Consider CD DG44 Medium when:
• You use parental DG44 or DXB11 CHO cells (dhfr-)

Design your own media specific to your needs
Whether it’s a bench-level experiment or large-scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco® cGMP Media Custom Services, you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.

Gibco® is a registered trademark of ThermoFisher Scientific Inc. Dynamis™ and CD FortiCHO™ are trademarks of Thermo Fisher Scientific Inc., ActiCHO(TM) is a trademark of GE Healthcare Biosciences AB, BalanCD(R) is a registered trademark of Irvine Scientific Sales Co., Cellvento(R) is a registered trademark of Merck KGAA, EXCELL(R) is a registered trademark of SAFC, HyCell(R) is a registered trademark of GE Healthcare Biosciences AB, and PowerCHO(TM) is a registered trademark of Lonza.

OneFeed™ Supplement (Gibco™)

OneFeed Supplement is a versatile medium supplement. OneFeed Supplement is a chemically-defined, animal-free, protein-free feed shown to enhance protein production when used in a variety of commercially available medium.