Shop All Basal Media

RPMI, no methionine (Gibco™)

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 medium has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of Gibco® RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using our media selector tool.

This RPMI medium is modified as follows:


WithWithout
• L-glutamine• Methionine
• Phenol red• HEPES


Gibco® RPMI 1640 medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.

Product Intended Use
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

cGMP Manufacturing and Quality System
This Gibco® RPMI medium is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

RPMI 1640 medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 medium requires supplementation, commonly with 10% fetal bovine serum (FBS). RPMI 1640 medium uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

MEM, high glucose, high sodium bicarbonate (Gibco™)

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco® MEM modifications for a range of cell culture applications. Find the right formulation using our media selector tool.

This MEM is modified as follows:


WithWithout
• Phenol red• L-glutamine
• 6 g/L D-glucose• HEPES
• 3.7 g/L sodium bicarbonate 


Gibco® MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow's MEM, MEM α, DMEM, and Temin's Modification. MEM is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.

Product Intended Use
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

cGMP Manufacturing and Quality System
Gibco® MEM is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% fetal bovine serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Opti-MEM™ I Reduced Serum Medium, no phenol red (Gibco™)

Opti-MEM® I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in cell growth rate or morphology. Opti-MEM® I medium is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM® I medium can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. We offer a variety of Opti-MEM® I Reduced-Serum Medium modifications for a range of cell culture applications.

This Opti-MEM® is modified as follows:
WithWithout
• L-glutamine• Phenol Red

The complete formulation is confidential. For more information, please contact Technical Services.

Using Opti-MEM® Reduced-Serum Medium
Opti-MEM® I medium is a unique medium that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%. Opti-MEM® I medium uses a sodium bicarbonate buffer system (2.4 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

cGMP manufacturing and quality system
For supply chain continuity, we manufacture Opti-MEM® Reduced-Serum Medium at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.

MEM α, nucleosides, no phenol red (Gibco™)

Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of Gibco® MEM α modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM α is modified as follows:

With Without
• Ribonucleosides • Phenol Red
• Deoxyribonucleosides
• L-glutamine

The complete formulation is available.

Gibco® MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR negative cells. This product is made with Earle’s salts.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

cGMP Manufacturing and Quality System
Gibco® MEM α is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

RPMI 1640 Medium, HEPES, no glutamine (Gibco™)

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of Gibco® RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This RPMI 1640 is modified as follows:
WithWithout
• Phenol Red• L-glutamine
• HEPES

The complete formulation is available.

Gibco® RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12, and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.

cGMP Manufacturing and Quality System
Gibco® RPMI 1640 is manufactured at a cGMP compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

MEM, no glutamine, no phenol red (Gibco™)

MEM (Minimum Essential Medium) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM is modified as follows:
Without
• L-glutamine
• HEPES
• Phenol Red

The complete formulation is available.

Using MEM
MEM was developed by Harry Eagle, and was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks' salts for use without CO2. This product is made with Earle’s salts. MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

cGMP manufacturing and quality system
MEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical MEM product made in our Scotland facility (51200-046). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

DMEM, high glucose, no glutamine (Gibco™)

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:
WithWithout
• High Glucose• L-glutamine
• Phenol Red• Sodium Pyruvate
• HEPES

The complete formulation is available.

Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Product use
For human ex vivo tissue and cell culture processing applications. CAUTION: When used as a medical device, Federal law restricts this device to sale by or on the order of a physician. Customers using Gibco® DMEM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP manufacturing and quality system
DMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

DMEM, high glucose, no glutamine, no phenol red (Gibco™)

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:
WithWithout
• High Glucose• L-glutamine
• Phenol Red
• Sodium Pyruvate
• HEPES

The complete formulation is available.

Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

cGMP manufacturing and quality system
DMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Medium 199, Hanks' Balanced Salts (Gibco™)

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco® Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This Medium 199 is modified as follows:
With
• Hanks' salts
• L-glutamine
• HEPES
• Phenol Red

The complete formulation is available.

Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. This Medium 199 formulation contains Hanks' salts for use without CO2.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco® Medium 199 is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco® Medium 199 product made in our Scotland facility (22350-029). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Medium 199 contains no proteins, lipids, or growth factors. Therefore, Medium 199 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). This Medium 199 formulation does not require a CO2 environment to maintain physiological pH.

Ham's F-10 Nutrient Mix, GlutaMAX™ Supplement (Gibco™)

Ham's F-10 Nutrient Mixture (F-10) was designed for serum-free growth of Chinese Hamster Ovary (CHO) cells. F-10 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including COS-7, primary rat astrocytes, and rat prostate epithelial cells.

This F-10 is modified as follows:

WithWithout
• GlutaMAX™• HEPES
• Phenol Red

The complete formulation is available.

Compared to other basal media, F-10 contains a wider variety of components, including zinc, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-10 has led to a variety of improved formulations, such as Ham's F-12 Nutrient Mixture (F-12).

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco® F-10 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Gibco® F-10 contains no proteins or growth factors. Therefore, F-10 often requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-10 uses a sodium bicarbonate buffer system (1.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Advanced MEM (Gibco™)

Advanced MEM (Minimum Essential medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic MEM, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology.i Cells successfully cultured in Advanced DMEM / F-12, with no adaptation, include PK-15, MDCK, Vero, WI-38 and Hep-2.

This Advanced MEM is manufactured as follows:

WithWithout
• Glucose• L-glutamine
• Non Essential Amino Acids• HEPES
• Sodium pyruvate
• Phenol Red

The complete formulation is available.

Gibco® Advanced MEM is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX® I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride.

Product Use
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

cGMP Manufacturing and Quality System
Gibco® Advanced MEM is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

Advanced MEM requires supplementation with 1-5% Fetal Bovine Serum and 4mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

iPaul Price, Brad Stiles, David Staines, Ethel Evege, Femi Fatunmbi, Kate Wagner, Lori Nestler, David Jayme. Advanced Media as Alternatives to Classical Basal Media. FOCUS, 2003. 25.2:3-6.

MEM, autoclavable, no glutamine, powder (Gibco™)

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco® MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM is modified as follows:

With Without
• Phenol Red • L-glutamine
• Earle's salts • HEPES
• Sodium Bicarbonate

The complete formulation is available.

Gibco® MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. To allow autoclaving this medium, choline chloride is substituted with choline bitartrate, phenol red is reduced, and succinic acid is added to prevent precipitation at low pH. This MEM formulation contains Earle’s salts for use in a CO2 incubator. This product is made with Earle’s salts.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

Dual-Site cGMP Manufacturing
For supply chain continuity, we manufacture Gibco® MEM at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.

MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

This autoclavable form of Gibco® cell culture medium requires pH adjustment to 4.1 - 4.2 before autoclaving, following by sodium bicarbonate and L-glutamine supplementation and final pH adjustment to 7.2 – 7.4 (see protocol for details).

DMEM, powder, high glucose (Gibco™)

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:
WithWithout
• High Glucose• Sodium Pyruvate
• L-glutamine• HEPES
• Phenol Red• Sodium Bicarbonate

The complete formulation is available.

Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH. Powder forms of Gibco® cell culture media require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

cGMP manufacturing and quality system
DMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable DMEM product made in our Scotland facility (52100-021). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Advanced DMEM (Gibco™)

Advanced DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM, serum supplementation can be reduced by 50–90% with no change in growth rate or morphology. Cells successfully cultured in Advanced DMEM, with no adaptation, include MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero.

This Advanced DMEM is manufactured as follows:
WithWithout
• High glucose• L-glutamine
• Non Essential Amino Acids
• Sodium pyruvate
• Phenol Red

The complete formulation is available.

Using Advanced DMEM
Advanced DMEM is unique from other media due to the addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX® I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride. Advanced DMEM requires supplementation with 1–5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

cGMP manufacturing and quality system
For supply chain continuity, we manufacture Advanced DMEM at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.

DMEM/F-12, HEPES, no phenol red (Gibco™)

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM/F-12 is modified as follows:
WithWithout
• L-glutamine• Phenol Red
• HEPES

The complete formulation is available.

Using DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH.

cGMP manufacturing and quality system
For supply chain continuity, we manufacture DMEM/F-12 at two separate facilities, located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.