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NanoOrange™ Protein Quantitation Kit, 200-2,000 assays (Invitrogen™)

The NanoOrange Protein Quantitation Kit contains a very sensitive and easy assay for protein quantitation, with detection as low as 10 ng/mL of protein in solution. This fluorescent dye is suitable for use with spectrofluorometers and microplate readers. For detection of lipoproteins or proteins in a complex lipid environment, check out our CBQCA Protein Quantitation Kit (C-6667).

Pierce™ Peroxide Assay Reagent B (Thermo Scientific™)

Thermo Scientific Pierce Peroxide Assay Reagent B is a formulation consisting of 125µM xylenol orange in water with sorbitol. This formulation is one of the component of Quantitative Peroxide Assay Kit used for detecting and measuring hydrogen peroxide levels based on the oxidation of ferrous to ferric ion in the presence of xylenol orange, present in the biological and other liquid samples.

Features of Peroxide Assay Reagent B:

Convenient—no proprietary reagents involved and remove the variability, difficulty and added expense of formulating the reagents yourself
Aqueous-compatible formulations— the formulation includes sorbitol, which provides enhanced sensitivity with non-lipid samples

Applications of Pierce Quantitative Peroxide Assay Kits:
• General hydrogen peroxide (H2O2) measurement in protein samples
• Quantitation of peroxides in detergents
• Assessment of the effects of peroxides on cellular activity
• Determination of protein glycation; Measurement of peroxide accumulation in lipids

In these assay kits, hydroperoxides convert the Fe2+ to Fe3+ at acidic pH. With the aqueous-compatible formulation, peroxide first reacts with sorbitol, converting it to a peroxyl radical, which in turn initiates Fe2+ oxidation to Fe3+. In the lipid compatible formulation, the peroxide converts the Fe2+ to Fe3+ directly. In a sulfuric acid solution, the Fe3+ complexes with the xylenol orange (XO) dye to yield a purple product having an absorbance maximum at 560nm. Peroxide levels in test samples can be determined by calculation from the know extinction coefficient of the XO-Fe complex or by reference to a standard curve prepared with hydrogen peroxide solution.Most proteins do not interfere with these assays, although some metal chelators may require use of a blank (i.e., control) to mitigate these effects.

Related Products
Pierce™ Quantitative Peroxide Assay Kit (Aqueous)
Pierce™ Quantitative Peroxide Assay Kit (Lipid)
Pierce™ Peroxide Assay Reagent A
Pierce™ Peroxide Assay Reagent C

Pierce™ Rapid Antibody Isotyping Kit plus Kappa and Lambda - Mouse (Thermo Scientific™)

The Thermo Scientific Pierce Rapid Mouse Antibody Isotyping Kit Plus Kappa and Lambda is a single-use, cassette-based kit for 5-minute lateral-flow assay determination of mouse monoclonal antibody class and subclass identity.

This antibody isotyping kit uses cartridges that provide a color-readout of the monoclonal antibody isotype within five minutes after pipetting a small amount of diluted culture supernatant or ascites fluid sample into the sample well. The kit determines mouse IgG1, IgG2a, IgG2b, IgG3, IgA, IgM, as well as light-chain identity (kappa vs. lambda light chains).

Features of the Pierce Rapid Mouse Antibody Isotyping Kit:

Long shelf life—stable for at least one year at room temperature
Single-step—add diluted antibody sample to the loading-well of cassette
Fast—within 5 minutes a band appears that indicates the antibody isotype
Sensitive—detect antibodies in tissue culture media or ascites fluid samples at any concentration greater than 10 ng/mL
Specific—assay does not cross-react with fetal bovine serum (FBS)
Reliable—results are similar to the standard ELISA-based isotyping assays

Each Pierce Rapid Isotyping Kit assay is performed by simply adding a properly diluted tissue culture supernatant or mouse ascites sample to the well of the small cassette. Gold conjugates embedded in the cassette form specific class- and subclass-soluble complexes with the antibodies in the sample. These complexes travel the length of the membrane and are resolved on the anti-isotype and class-specific antibody-impregnated membrane. Results are displayed as a red band indicating the antibody isotype or subclass.

Determining the class and subclass of a monoclonal antibody is useful in planning the best immunoglobulin purification method. For example, mouse IgA and IgM are best purified by size (i.e., gel exclusion) or using immunoaffinity separation columns. Mouse IgG2a and IgG2b are purified with immobilized Protein A at pH 7-8, while Mouse IgG1 binds best to Protein A at pH 8-9. Immunoglobulin that contains kappa light chains can be purified using immobilized Protein L.

The Pierce Rapid Isotyping Kit is compatible with both tissue culture supernatant and mouse ascites fluid, and is more sensitive than conventional latex-bead based dipstick assays and much faster than ELISA-based isotyping assays. The kit requires only 0.5µL ascites fluid, 5 to 55µL of cell culture supernatant or 1.5ng of purified antibody.

Related Products
Rapid ELISA Mouse mAb Isotyping Kit
Pierce™ Rapid Antibody Isotyping Kit - Mouse

ProQuest™ Two-Hybrid System with Gateway™ Technology (Invitrogen™)

The ProQuest™ Two-Hybrid System with Gateway® Technology is an in vivo yeast-based system for identifying interactions between two proteins. This interaction reconstitutes a functional transcription factor that activates chromosomally integrated reporter genes where transcriptional activation is monitored by the growth of cells on selective media.
The ProQuest™ System features:
• Gateway® Technology to allow rapid and easy generation of bait and prey constructs, and to facilitate downstream application
• Low copy-number DBD (DNA Binding Domain) and AD (Activation Domain) vectors (ARS/CEN) to control over-expression and increase reproducibility
• Three different reporter genes (HIS3, URA3, and lacZ) with independent promoter regions to rapidly weed out false positives. URA3 reporter gene allows for both positive and negative selection, enabling advanced two-hybrid techniques such as reverse two-hybrid.

• A set of strong-to-weak Gateway®-based control interactions to evaluate results and assess the strength of the interaction being tested

Pro-Detect™ Rapid Mouse Fc Assay Kit (Thermo Scientific™)

The Thermo Scientific Pro-Detect Rapid Mouse Fc Assay Kit is a single-use, dipstick lateral flow kit for quick, easy detection of mouse Fc-tagged proteins in protein expression and purification systems. This lateral flow assay uses membrane-based strips coated with dye-bound conjugated antibodies specific to the mouse Fc tag to provide a visual, color readout for detection within ten minutes. This assay can be used to detect mouse Fc-tagged proteins directly from cell culture media (supernatant) or cell lysates without any special instrumentation or handling.

Features of the Pro-Detect Rapid assay kits:
Easy to adopt—lateral flow strips can be used early in and throughout expression to monitor and confirm expression of desired protein-tagged target
Simple, fast method—simply dilute sample using provided diluent and dip in the lateral flow strip for visual results within 5-10 minutes
Sensitive—detect protein tags in supernatant or cell lysate samples down to low µg/mL concentrations
Specific—assay does not cross-react with common lysis and protein preparation reagents
Reliable—results are similar to other antibody immunoassays, including western blot and ELISA
Long shelf life—stable for at least one year at 4°C

Each Pro-Detect Rapid assay is performed by simply adding a properly diluted tissue culture supernatant, lysate, or purified protein sample to a test tube or microplate well and dipping in a lateral flow strip. Gold-conjugated detection antibodies embedded in the strip form complexes with the tagged protein in the sample. These complexes travel the length of the strip membrane and bind at the test and control lines. Results are displayed as red bands.

96-Well Plates for Pierce™ BCA-RAC Assay (Thermo Scientific™)

These are polystyrene 96-well round-bottom plates suitable for use with Thermo Scientific BCA-RAC (reducing agent compatible) assays.

Related Products
Pierce™ Microplate BCA Protein Assay Kit - Reducing Agent Compatible
Pierce™ BCA Protein Assay Kit - Reducing Agent Compatible

Pierce™ Detergent Compatible Bradford Assay Reagent (Thermo Scientific™)

The Thermo Scientific™ Pierce™ Detergent Compatible Bradford Assay Reagent is a quick and ready-to-use modification of the well-known Bradford coomassie dye-binding, colorimetric method for total protein quantitation. Proprietary additives to the Bradford Reagent make it compatible with up to 1% or higher of detergents and lysis reagents that are commonly used in life science research, including Triton® X-100 and NP-40.

• Convenient—detergent-free standard curve
• Flexible—compatible with samples both with and without detergent
• Minimal sample—requires only 10 µL for microplate procedure
• Colorimetric—measure with a standard spectrophotometer or plate reader (595 nm)
• Easy to use—single reagent, no working reagent preparation required
• Fast—10 minute incubation at room temperature
• Broad range—detects protein concentration in the ranges of 2 to 1500 µg/mL

Similar to the Bradford method, coomassie dye binds protein in an acidic medium causing an immediate shift in absorption maximum from 465 nm to 595 nm with a concomitant color change from green to blue. In addition, the assay is complete in just 10 minutes.

The protein assay can be performed in either test tube or microplate format. The standard working range is 100-1500 µg/mL with up to 1% detergent (or higher in some cases). Protein concentrations are estimated by reference to absorbances obtained for a series of standard protein dilutions, typically bovine serum albumin (BSA), which are assayed alongside the unknown samples. Because the color response with coomassie dye is non-linear with increasing protein concentration, a standard curve must be completed with each assay. Standards can be used directly without preparing them in the same detergent found in the test samples.

Pro-Detect™ Rapid AVI Competitive Assay Kit (Thermo Scientific™)

The Thermo Scientific Pro-Detect Rapid AVI Competitive Assay Kit is a single-use, dipstick lateral flow kit for quick, easy detection of AVI (sequence: GLNDIFEAQKIEWHE)-tagged proteins in protein expression and purification systems. This lateral flow assay uses membrane-based strips coated with dye-bound conjugated antibodies specific to AVI tag to provide a qualitative detection within ten minutes. This assay can be used to detect AVI-tagged proteins directly from cell culture media (supernatant) or cell lysates without any special instrumentation or handling.

Features of the Pro-Detect Rapid competitive assay kits:
Easy to adopt—lateral flow strips can be used early in and throughout expression to monitor and confirm expression of desired protein-tagged target
Simple, fast method—simply dilute sample using provided diluent and dip in the lateral flow strip for qualitative results within 5-10 minutes
Sensitive—detect protein tags in supernatant or cell lysate samples down to low µg/mL concentrations
Specific—assay does not cross-react with common lysis and protein preparation reagents
Reliable—results are similar to other antibody immunoassays, including western blot and ELISA
Long shelf life—stable for at least one year at 4°C

Each Pro-Detect Rapid competitive assay is performed by simply adding a properly diluted tissue culture supernatant, lysate, or purified protein sample to a test tube or microplate well and dipping in a lateral flow strip. Gold-conjugated detection antibodies embedded in the strip form complexes with the tagged protein in the sample. In the presence of target protein tag, the dye-bound conjugated antibodies will bind to the tagged protein present in the sample instead of the protein tag bound at the test lines. Therefore, in a positive result, a competitive lateral flow assay will show decreasing intensity of the visible test lines until all test lines have disappeared. When there is no target protein tag present, the dye-bound conjugated antibodies specific to the target will bind at the test line(s), resulting in visual test lines in a negative result.

InsuQuant Mass Spectrometric Kit (Thermo Scientific™)

Simplify your affinity purification workflow with the Thermo Scientific™ InsuQuant™ Mass Spectrometric Kit, an exclusive pre-analytical solution designed to simultaneously detect, differentiate, and quantify endogenous and exogenous insulin types.

Easy-Titer™ Human IgG (H+L) Assay Kit (Thermo Scientific™)

The Thermo Scientific Easy-Titer Human IgG (H+L) Assay Kit includes antibody-sensitized microspheres to measure the specific concentration of antibodies by an easy and rapid microagglutination technique using standard microplates and UV-Vis plate reader (spectrophotometer). This kit is specific for human IgG and, unlike total protein assays, can specifically measure the concentration of target antibody in samples (e.g., serum, plasma, culture supernatant) that contain other proteins. It is sensitive, requiring very small sample volumes. Antibody concentration is determined from the assay response (absorbance) by comparison to a standard curve prepared using dilutions of a known antibody sample (sold separately).

General features of Easy-Titer Antibody Assay Kits:

Antibody-based specificity—measure concentration of target antibody in a sample, not just total protein; no need to purify antibody to assess its concentration
Faster and easier than ELISA—three-component, homogenous assay; 10 minutes total incubation time
No special equipment needed—uses standard vortex mixer, pipetter, 96-well microplate, plate shaker and reader (measure absorbance at 340nm or 400nm)
Sensitive—assay range (standard curve) 8 to 500 ng/mL; use sample at 15 to 300 ng/mL for optimal results
Reproducible—coefficient of variation < 5%; error depends on dilution and pipetting technique
Antibody standards sold separately—see product list for suggested products; use any antibody standard with proper target identity and known concentration (greater than 10 µg/mL)
Kits for five popular targets—choose a kit specific for a particular species and class of immunoglobulin; no cross-reaction with other species and classes of the target antibody

Easy-Titer Assay Kits detect and measure specific target antibodies using agglutination of microspheres that are coated ("sensitized") with the specific anti-IgG or IgM polyclonal antibodies. In the appropriate aqueous buffer (supplied in kit), the monodispersed antibody-coated microspheres (> 1 µM diameter) have highest absorptivity (λ-max) to incident light having a wavelength (340nm) that is equal to approximately half their diameter. When sample is added, two or more microspheres bind to each antibody target via their coated specific polyclonal antibodies, and this agglutination into effectively larger apparent spheres results in proportional decrease in absorptivity (lower absorbance).

Typical microagglutination assays depend on a change in light-scattering and corresponding change in transmittance, to which absorbance is inversely related. Easy-Titer Assay Kits use a special dilution buffer whose refractive index eliminates the effect of light-scattering on the monodispersed microspheres for the measurement wavelength used. Because of this, the final 10- to 20-fold dilution of the sample for use in the assay must be done using the Dilution Buffer supplied in the kit.

Easy-Titer Antibody Assays are faster and easier than ELISA:
• Prepare standards (5 to 500 ng/mL) by diluting purified antibody in Kit Dilution Buffer.
• Prepare samples by diluting in Dilution Buffer to within assay range (8 to 500 ng/mL).
• Vortex vial of microsphere beads to create homogeneous suspension.
• Pipette 20 µL of bead suspension and 20 µL of each sample and standard into 96-well microplate wells.
• Incubate microplate for 5 minutes with vigorous mixing.
• Add 100 µL of Kit Blocking Reagent.
• Incubate microplate for 5 minutes with vigorous mixing.
• Measure absorbance on standard plate reader (340nm or 405nm).
• Plot standard curve and interpolate samples to determine concentration.

Related Products
Easy-Titer™ Mouse IgG Assay Kit
Easy-Titer™ Rabbit IgG Assay Kit
Easy-Titer™ Human IgG (gamma chain) Assay Kit
Easy-Titer™ Human IgM Assay Kit

Easy-Titer™ Human IgM Assay Kit (Thermo Scientific™)

The Thermo Scientific Easy-Titer Human IgM Assay Kit includes antibody-sensitized microspheres to measure the specific concentration of antibodies by an easy and rapid microagglutination technique using standard microplates and UV-Vis plate reader (spectrophotometer). This kit is specific for human IgM and, unlike total protein assays, can specifically measure the concentration of target antibody in samples (e.g., serum, plasma, culture supernatant) that contain other proteins. It is sensitive, requiring very small sample volumes. Antibody concentration is determined from the assay response (absorbance) by comparison to a standard curve prepared using dilutions of a known antibody sample (sold separately).

General features of Easy-Titer Antibody Assay Kits:

Antibody-based specificity—measure concentration of target antibody in a sample, not just total protein; no need to purify antibody to assess its concentration
Faster and easier than ELISA—three-component, homogenous assay; 10 minutes total incubation time
No special equipment needed—uses standard vortex mixer, pipetter, 96-well microplate, plate shaker and reader (measure absorbance at 340nm or 400nm)
Sensitive—assay range (standard curve) 8 to 500 ng/mL; use sample at 15 to 300 ng/mL for optimal results
Reproducible—coefficient of variation < 5%; error depends on dilution and pipetting technique
Antibody standards sold separately—see product list for suggested products; use any antibody standard with proper target identity and known concentration (greater than 10 µg/mL)
Kits for five popular targets—choose a kit specific for a particular species and class of immunoglobulin; no cross-reaction with other species and classes of the target antibody

Easy-Titer Assay Kits detect and measure specific target antibodies using agglutination of microspheres that are coated ("sensitized") with the specific anti-IgG or IgM polyclonal antibodies. In the appropriate aqueous buffer (supplied in kit), the monodispersed antibody-coated microspheres (> 1 µM diameter) have highest absorptivity (λ-max) to incident light having a wavelength (340nm) that is equal to approximately half their diameter. When sample is added, two or more microspheres bind to each antibody target via their coated specific polyclonal antibodies, and this agglutination into effectively larger apparent spheres results in proportional decrease in absorptivity (lower absorbance).

Typical microagglutination assays depend on a change in light-scattering and corresponding change in transmittance, to which absorbance is inversely related. Easy-Titer Assay Kits use a special dilution buffer whose refractive index eliminates the effect of light-scattering on the monodispersed microspheres for the measurement wavelength used. Because of this, the final 10- to 20-fold dilution of the sample for use in the assay must be done using the Dilution Buffer supplied in the kit.

Easy-Titer Antibody Assays are faster and easier than ELISA:
• Prepare standards (5 to 500 ng/mL) by diluting purified antibody in Kit Dilution Buffer.
• Prepare samples by diluting in Dilution Buffer to within assay range (8 to 500 ng/mL).
• Vortex vial of microsphere beads to create homogeneous suspension.
• Pipette 20 µL of bead suspension and 20 µL of each sample and standard into 96-well microplate wells.
• Incubate microplate for 5 minutes with vigorous mixing.
• Add 100 µL of Kit Blocking Reagent.
• Incubate microplate for 5 minutes with vigorous mixing.
• Measure absorbance on standard plate reader (340nm or 405nm).
• Plot standard curve and interpolate samples to determine concentration.

Related Products
Easy-Titer™ Mouse IgG Assay Kit
Easy-Titer™ Rabbit IgG Assay Kit
Easy-Titer™ Human IgG (H+L) Assay Kit
Easy-Titer™ Human IgG (gamma chain) Assay Kit

Pierce™ Coomassie Plus (Bradford) Assay Reagent (Thermo Scientific™)

The Thermo Scientific Pierce Coomassie Plus Protein Assay is a ready-to-use, reducing agent-compatible, improved Bradford assay reagent to quickly measure (A595nm) total protein concentration compared to a protein standard.

The Pierce Coomassie Plus Assay Reagent is a single, ready-to-use solution for measuring protein concentration. Simply add the reagent to equal volumes of samples and standards, mix and then measure the absorbance at 595nm. The assay costs only pennies per sample and can be performed in either test tube or microplate format. The Pierce Coomassie Plus Assay Reagent provides increased linearity of response and only half the protein-to-protein variability of other commercial Bradford assay formulations.

Features of the Coomassie Plus Protein Assay:

Colorimetric—measure with a standard spectrophotometer or plate reader (595nm)
Easy to use—single reagent; no working reagent preparation required
Fast—almost immediate color development; add, mix and read results
Broad range—detects protein concentration in the range 1 to 1500 µg/mL
Better—improved linearity and response-uniformity compared to traditional Bradford formulations
Flexible—microplate and cuvette protocols provided with the instructions and adaptable to several target working ranges

How the Coomassie Plus (Bradford) Assay Detects Protein
Use of coomassie G-250 dye in a colorimetric reagent for the detection and quantitation of total protein was first described by Dr. Marion Bradford in 1976. In the acidic environment of the reagent, protein binds to the coomassie dye. This results in a spectral shift from the reddish/brown form of the dye (absorbance maximum at 465nm) to the blue form of the dye (absorbance maximum at 610nm). The difference between the two forms of the dye is greatest at 595nm, so that is the optimal wavelength to measure the blue color from the coomassie dye-protein complex. If desired, the blue color can be measured at any wavelength between 575nm and 615nm. At the two extremes (575nm and 615nm) there is a loss of about 10% in the measured amount of color (absorbance) compared to that obtained at 595nm.

Development of color in coomassie dye-based (Bradford) protein assays has been associated with the presence of certain basic amino acids (primarily arginine, lysine and histidine) in the protein. Van der Waals forces and hydrophobic interactions also participate in the binding of the dye by protein. The number of Coomassie dye ligands bound to each protein molecule is approximately proportional to the number of positive charges found on the protein. Free amino acids, peptides and low molecular weight proteins do not produce color with coomassie dye reagents. In general, the mass of a peptide or protein must be at least 3,000 daltons to be assayed with this reagent. The assay is performed at room temperature and no special equipment is required. Simply add the sample to the tube containing reagent and the resultant blue color is measured at 595nm following a short room-temperature incubation. The coomassie dye containing protein assay is compatible with most salts, solvents, buffers, thiols, reducing substances and metal chelating agents encountered in protein samples.

For more information, see the article "Chemistry of Protein Assays" in the Protein Methods Library.

Related Products
Pierce™ Coomassie Plus (Bradford) Assay Kit
Pierce™ Detergent Compatible Bradford Assay Kit

Easy-Titer™ Human IgG (gamma chain) Assay Kit (Thermo Scientific™)

The Thermo Scientific Easy-Titer Human IgG (gamma chain) Assay Kit includes antibody-sensitized microspheres to measure the specific concentration of antibodies by an easy and rapid microagglutination technique using standard microplates and UV-Vis plate reader (spectrophotometer). This kit is specific for the human IgG gamma chain and, unlike total protein assays, can specifically measure the concentration of target antibody in samples (e.g., serum, plasma, culture supernatant) that contain other proteins. It is sensitive, requiring very small sample volumes. Antibody concentration is determined from the assay response (absorbance) by comparison to a standard curve prepared using dilutions of a known antibody sample (sold separately).

General features of Easy-Titer Antibody Assay Kits:

Antibody-based specificity—measure concentration of target antibody in a sample, not just total protein; no need to purify antibody to assess its concentration
Faster and easier than ELISA—three-component, homogenous assay; 10 minutes total incubation time
No special equipment needed—uses standard vortex mixer, pipetter, 96-well microplate, plate shaker and reader (measure absorbance at 340nm or 400nm)
Sensitive—assay range (standard curve) 8 to 500 ng/mL; use sample at 15 to 300 ng/mL for optimal results
Reproducible—coefficient of variation < 5%; error depends on dilution and pipetting technique
Antibody standards sold separately—see product list for suggested products; use any antibody standard with proper target identity and known concentration (greater than 10 µg/mL)
Kits for five popular targets—choose a kit specific for a particular species and class of immunoglobulin; no cross-reaction with other species and classes of the target antibody

Easy-Titer Assay Kits detect and measure specific target antibodies using agglutination of microspheres that are coated ("sensitized") with the specific anti-IgG or IgM polyclonal antibodies. In the appropriate aqueous buffer (supplied in kit), the monodispersed antibody-coated microspheres (> 1 µM diameter) have highest absorptivity (λ-max) to incident light having a wavelength (340nm) that is equal to approximately half their diameter. When sample is added, two or more microspheres bind to each antibody target via their coated specific polyclonal antibodies, and this agglutination into effectively larger apparent spheres results in proportional decrease in absorptivity (lower absorbance).

Typical microagglutination assays depend on a change in light-scattering and corresponding change in transmittance, to which absorbance is inversely related. Easy-Titer Assay Kits use a special dilution buffer whose refractive index eliminates the effect of light-scattering on the monodispersed microspheres for the measurement wavelength used. Because of this, the final 10- to 20-fold dilution of the sample for use in the assay must be done using the Dilution Buffer supplied in the kit.

Easy-Titer Antibody Assays are faster and easier than ELISA:
• Prepare standards (5 to 500 ng/mL) by diluting purified antibody in Kit Dilution Buffer.
• Prepare samples by diluting in Dilution Buffer to within assay range (8 to 500 ng/mL).
• Vortex vial of microsphere beads to create homogeneous suspension.
• Pipette 20 µL of bead suspension and 20 µL of each sample and standard into 96-well microplate wells.
• Incubate microplate for 5 minutes with vigorous mixing.
• Add 100 µL of Kit Blocking Reagent.
• Incubate microplate for 5 minutes with vigorous mixing.
• Measure absorbance on standard plate reader (340nm or 405nm).
• Plot standard curve and interpolate samples to determine concentration.

Related Products
Easy-Titer™ Mouse IgG Assay Kit
Easy-Titer™ Rabbit IgG Assay Kit
Easy-Titer™ Human IgG (H+L) Assay Kit
Easy-Titer™ Human IgM Assay Kit

EZQ™ Protein Quantitation Kit (Invitrogen™)

The EZQ Protein Quantitation Kit provides a fluorescence-based protein assay that facilitates fast quantitation of protein samples prepared for gel electrophoresis. The assay can be performed in the presence of detergents, urea and reducing agents—simply spot 1 µL of your protein sample onto the prepared paper, stain with our proprietary fluorescent dye, and measure the fluorescence. Samples can be quantitated by comparison with a standard curve. For added versatility, we provide a specially-designed 96-well microplate for easy quantitation of samples on a microplate reader or a laser scanner.

Micro BCA™ Protein Assay Kit (Thermo Scientific™)

The Thermo Scientific Pierce Micro BCA Protein Assay Kit is a special 3-component version of our popular BCA Reagents, optimized to measure (A562nm) total protein concentration of dilute protein solutions (0.5 to 20 micrograms/mL).

Features of the Micro BCA Protein Assay Kit:

Sensitive—accurately detect down to 0.5 µg/mL (2 µg/mL in microplate format)
High linearity—linear working range for BSA equals 0.5 to 20 µg/mL
Colorimetric—measure with a standard spectrophotometer or plate reader (562nm)
Compatible—unaffected by typical concentrations of most ionic and nonionic detergents
Convenient—microplate and cuvette protocols provided with the instructions
Stable—kit stable at room temperature; prepared working reagent stable for to 24 hours

The Micro BCA Protein Assay Kit is a specialized version of the popular Pierce BCA Protein Assay for determining the protein concentration of dilute samples. Mixing together the three Micro BCA Reagents results in a working solution that is sufficiently concentrated to measure protein when mixed with an equal volume of sample. The result is an assay for accurately measuring 0.5 to 20 µg/mL protein solutions. The assay is exceptionally linear and exhibits very low levels of protein-to-protein variability.

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Micro BCA™ Reagent A (MA)
Micro BCA™ Reagent B (MB)
Micro BCA™ Reagent C (MC)
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