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Fischer's Medium (Gibco™)

Fischer's medium was originally developed by Glen Fischer at the National Cancer Institute (Bethesda, MD) and formulated for the culture of mouse leukemic cells. Fischer's Medium is primarily used for the culture of hematopeitic cells, such as lymphoblasts and lymphocytes.

This Fischer's medium is manufactured as follows:
WithWithout
• L-glutamine• HEPES
• Phenol Red

The complete formulation is available.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law.

cGMP Manufacturing
Gibco® Fischer's medium is manufactured at a cGMP compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Fischer's medium contains no proteins, lipids, or growth factors. Fischer's medium typically requires supplementation of Fetal Bovine Serum (FBS). Fischer's medium uses a sodium bicarbonate buffer system (1.125 g / L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

NCTC-135 Medium (Gibco™)

NCTC-135 medium is a chemically defined medium that was originally developed at the National Cancer Institute (Bethesda, MD) and formulated for the culture of mouse Strain L sublines, NCTC clone 929 (L929 cells) in a serum-free environment. Gibco® NCTC-135 medium has also been used to grow many cells, including HeLa, Ehrlich-Lettre Ascites strain E, NCTC clone 1469, NCTC 4206, mouse C3H, BS-C-1, and hybridoma cell lines.
This NCTC-135 is modified as follows:
WithWithout
• L-glutamine• HEPES
• Phenol Red
• Coenzymes

The complete formulation is available.

Gibco® NCTC-135 is a modification of NCTC 109 medium that replaced L-cysteine with L-cystine, which reduced toxicity to some cells. NCTC-135 medium is rich in nucleosides contains several unique components to eliminate the need for serum, including coenzymes, glucosamine, reducing agents and additional vitamins.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco® NCTC-135 medium is manufactured at a cGMP compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

NCTC-135 medium contains no proteins, lipids, or growth factors. NCTC-135 medium was designed for use without serum for L929 cells, but typically requires supplementation of a growth factor cocktail or Fetal Bovine Serum (FBS) for other cells. NCTC-135 medium uses a sodium bicarbonate buffer system (2.2 g / L) and therefore requires a 5-10% CO2 environment to maintain physiological pH. Some cell lines might require gradual adaptation to a serum-free medium.

MEM Rega-3 (Gibco™)

Minimum Essential Medium (MEM) REGA-3 is a modification of the commonly used MEM. The Rega-3 modification is often used to culture HEP-G2 (human liver carcinoma) cells and occasionally other human tumor cells. We offer a variety of Gibco® MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM is modified as follows:

With Without
• Phenol Red • L-glutamine
• Gentamycin Sulfate • HEPES
• Sodium Bicarbonate

The complete formulation is available.

Gibco® MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including this Rega-3 modification. MEM Rega-3 is unique because it has a reduced sodium bicarbonate concentration, modified salt concentrations, and contains the antibiotic gentamycin sulfate. This product is made with Earle’s salts.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

cGMP Manufacturing and Quality System
Gibco® MEM is manufactured at a cGMP compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

MEM Rega-3 contains no proteins, lipids, or growth factors. Therefore, MEM Rega-3 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (0.85 g ⁄ L) and therefore requires a low CO2 environment to maintain physiological pH.