Shop All Other Basal Media

MEM Rega-3 (Gibco™)

Minimum Essential Medium (MEM) REGA-3 is a modification of the commonly used MEM. The Rega-3 modification is often used to culture HEP-G2 (human liver carcinoma) cells and occasionally other human tumor cells. We offer a variety of Gibco® MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM is modified as follows:

With Without
• Phenol Red • L-glutamine
• Gentamycin Sulfate • HEPES
• Sodium Bicarbonate

The complete formulation is available.

Gibco® MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including this Rega-3 modification. MEM Rega-3 is unique because it has a reduced sodium bicarbonate concentration, modified salt concentrations, and contains the antibiotic gentamycin sulfate. This product is made with Earle’s salts.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

cGMP Manufacturing and Quality System
Gibco® MEM is manufactured at a cGMP compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

MEM Rega-3 contains no proteins, lipids, or growth factors. Therefore, MEM Rega-3 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (0.85 g ⁄ L) and therefore requires a low CO2 environment to maintain physiological pH.

EfficientFeed™ C+ 2X Supplement (Gibco™)

Gibco EfficientFeed C+ 2X Supplement is a single-part liquid supplement for fed batch addition to the culture of multiple cell lines to assist with process development and maximization of bioreactor utilization. This product is designed to work with CHO cell lines expressing endogenous glutamine synthetase (GS) as a single feed without the need for additional GS supplements. Compared to the original CD EfficientFeed C AGT nutrient supplement, EfficientFeed C+ 2X Supplement has components that are super-concentrated from 81.2 g/L (1X AGT) to 162.4 g/L (2X), helping reduce product dilution or increase titers with additional feeding. The product is an animal origin-free, chemically defined formulation that contains no proteins, hydrolysates, or components of incompletely defined composition.

Which EfficientFeed+ supplement to choose?

Consider EfficientFeed A+ Supplement when:
• You have used CHO CD EfficientFeed A supplement in the past and wish to explore simpler reconstitution and super-concentration benefits during delivery of key components
• You are currently using CD OptiCHO as your base cell growth medium

Consider EfficientFeed B+ Supplement when:
• You have used CHO CD EfficientFeed B supplement in the past and wish to explore simpler reconstitution and super-concentration benefits during delivery of key components
• You are currently using CD CHO as your base cell growth medium

Consider EfficientFeed C+ Supplement when:
• You have used CD EfficientFeed C supplement in the past and wish to explore super-concentration benefits during delivery of key components
• You want higher productivity in GS CHO, CHO-K1, and CHO-S derived cell lines
• You are currently using CD FortiCHO as your base cell growth medium
• You are using a base medium with greater than 6 g/L glucose or a hydrolysate at Day 0

Increasing titers
As a complete feed, EfficientFeed+ supplements have been shown to provide multiple-fold increases in titer versus batch culture and are an excellent starting point for process development as ready-to-use single-part liquid feeds.

Maximizing bioreactor utilization
Have you ever wished for more working volume at the start of a bioreactor run to produce more protein, but need too much feeding volume space? Have you ever thought that room for more feeding might achieve a new level of titer from a process and maybe even reduce the number of bioreactor runs needed per year? Using super-concentrated ready-to-feed liquid feeding supplements helps reduce product dilution, increase space for additional feeding, and/or increase the working volume at the start of the run. In fact, you may gain about 20% more bioreactor utilization with this super-concentrated EfficientFeed+ product.

Simplified handling to reduce risk in scale-up
The ability to scale up in liquid or AGT dry format provides ease of handling and storage in manufacturing and other bioprocess settings. Fewer parts and steps in reconstitution mean fewer opportunities for operator error. Further, the ability to store this feed, even when super-concentrated, for the length of a bioreactor run at room temperature, in the dark, helps reduce chances of contamination during closed system connections by reducing the number of connections to the bioreactor to one.

EfficientFeed™ A+ 3X Supplement (Gibco™)

Gibco EfficientFeed A+ 3X Supplement is a single-part liquid supplement for fed batch addition to the culture of multiple cell lines to assist with process development and maximization of bioreactor utilization. Compared to original CHO CD EfficientFeed A AGT nutrient supplement, EfficientFeed A+ 3X Supplement has components that are super-concentrated from 66.4 g/L (1X AGT) to 199.2 g/L (3X), helping reduce product dilution or increase titers with additional feeding. The supplement is an animal origin-free, chemically defined formulation that contains no proteins, hydrolysates, or components of incompletely defined composition.

Which EfficientFeed+ supplement to choose?

Consider EfficientFeed A+ Supplement when:
• You have used CHO CD EfficientFeed A supplement in the past and wish to explore simpler reconstitution and super-concentration benefits during delivery of key components
• You are currently using CD OptiCHO as your base cell growth medium

Consider EfficientFeed B+ Supplement when:
• You have used CHO CD EfficientFeed B supplement in the past and wish to explore simpler reconstitution and super-concentration benefits during delivery of key components
• You are currently using CD CHO as your base cell growth medium

Consider EfficientFeed C+ Supplement when:
• You have used CD EfficientFeed C supplement in the past and wish to explore super-concentration benefits during delivery of key components
• You want higher productivity in GS CHO, CHO-K1, and CHO-S derived cell lines
• You are currently using CD FortiCHO as your base cell growth medium
• You are using a base medium with greater than 6 g/L glucose or a hydrolysate at Day 0

Increasing titers
As a complete feed, EfficientFeed+ supplements have been shown to provide multiple-fold increases in titer versus batch culture and are an excellent starting point for process development as ready-to-use single-part liquid feeds.

Maximizing bioreactor utilization
Have you ever wished for more working volume at the start of a bioreactor run to produce more protein, but need too much feeding volume space? Have you ever thought that room for more feeding might achieve a new level of titer from a process and maybe even reduce the number of bioreactor runs needed per year? Using super-concentrated ready-to-feed liquid feeding supplements helps reduce product dilution, increase space for additional feeding, and/or increase the working volume at the start of the run. In fact, you may gain about 20% more bioreactor utilization with this super-concentrated EfficientFeed+ product.

Simplified handling to reduce risk in scale-up
The ability to scale up in liquid or AGT dry format provides ease of handling and storage in manufacturing and other bioprocess settings. Fewer parts and steps in reconstitution mean fewer opportunities for operator error. Further, the ability to store this feed, even when super-concentrated, for the length of a bioreactor run at room temperature, in the dark, helps reduce chances of contamination during closed system connections by reducing the number of connections to the bioreactor to one.

NCTC-135 Medium (Gibco™)

NCTC-135 medium is a chemically defined medium that was originally developed at the National Cancer Institute (Bethesda, MD) and formulated for the culture of mouse Strain L sublines, NCTC clone 929 (L929 cells) in a serum-free environment. Gibco® NCTC-135 medium has also been used to grow many cells, including HeLa, Ehrlich-Lettre Ascites strain E, NCTC clone 1469, NCTC 4206, mouse C3H, BS-C-1, and hybridoma cell lines.
This NCTC-135 is modified as follows:
WithWithout
• L-glutamine• HEPES
• Phenol Red
• Coenzymes

The complete formulation is available.

Gibco® NCTC-135 is a modification of NCTC 109 medium that replaced L-cysteine with L-cystine, which reduced toxicity to some cells. NCTC-135 medium is rich in nucleosides contains several unique components to eliminate the need for serum, including coenzymes, glucosamine, reducing agents and additional vitamins.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco® NCTC-135 medium is manufactured at a cGMP compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

NCTC-135 medium contains no proteins, lipids, or growth factors. NCTC-135 medium was designed for use without serum for L929 cells, but typically requires supplementation of a growth factor cocktail or Fetal Bovine Serum (FBS) for other cells. NCTC-135 medium uses a sodium bicarbonate buffer system (2.2 g / L) and therefore requires a 5-10% CO2 environment to maintain physiological pH. Some cell lines might require gradual adaptation to a serum-free medium.

Fischer's Medium (Gibco™)

Fischer's medium was originally developed by Glen Fischer at the National Cancer Institute (Bethesda, MD) and formulated for the culture of mouse leukemic cells. Fischer's Medium is primarily used for the culture of hematopeitic cells, such as lymphoblasts and lymphocytes.

This Fischer's medium is manufactured as follows:
WithWithout
• L-glutamine• HEPES
• Phenol Red

The complete formulation is available.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law.

cGMP Manufacturing
Gibco® Fischer's medium is manufactured at a cGMP compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Fischer's medium contains no proteins, lipids, or growth factors. Fischer's medium typically requires supplementation of Fetal Bovine Serum (FBS). Fischer's medium uses a sodium bicarbonate buffer system (1.125 g / L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

EfficientFeed™ B+ 3X Supplement (Gibco™)

Gibco EfficientFeed B+ 3X Supplement is a single-part liquid supplement for fed batch addition to the culture of multiple cell lines to assist with process development and maximization of bioreactor utilization. Compared to original CHO CD EfficientFeed B AGT nutrient supplement, EfficientFeed B+ 3X Supplement has components that are super-concentrated from 52.7 g/L (1X AGT) to 158.1 g/L (3X), helping reduce product dilution or increase titers with additional feeding. The supplement is an animal origin-free, chemically defined formulation that contains no proteins, hydrolysates, or components of incompletely defined composition.

Which EfficientFeed+ supplement to choose?

Consider EfficientFeed A+ Supplement when:
• You have used CHO CD EfficientFeed A supplement in the past and wish to explore simpler reconstitution and super-concentration benefits during delivery of key components
• You are currently using CD OptiCHO as your base cell growth medium

Consider EfficientFeed B+ Supplement when:
• You have used CHO CD EfficientFeed B supplement in the past and wish to explore simpler reconstitution and super-concentration benefits during delivery of key components
• You are currently using CD CHO as your base cell growth medium

Consider EfficientFeed C+ Supplement when:
• You have used CD EfficientFeed C supplement in the past and wish to explore super-concentration benefits during delivery of key components
• You want higher productivity in GS CHO, CHO-K1, and CHO-S derived cell lines
• You are currently using CD FortiCHO as your base cell growth medium
• You are using a base medium with greater than 6 g/L glucose or a hydrolysate at Day 0

Increasing titers
As a complete feed, EfficientFeed+ supplements have been shown to provide multiple-fold increases in titer versus batch culture and are an excellent starting point for process development as ready-to-use single-part liquid feeds.

Maximizing bioreactor utilization
Have you ever wished for more working volume at the start of a bioreactor run to produce more protein, but need too much feeding volume space? Have you ever thought that room for more feeding might achieve a new level of titer from a process and maybe even reduce the number of bioreactor runs needed per year? Using super-concentrated ready-to-feed liquid feeding supplements helps reduce product dilution, increase space for additional feeding, and/or increase the working volume at the start of the run. In fact, you may gain about 20% more bioreactor utilization with this super-concentrated EfficientFeed+ product.

Simplified handling to reduce risk in scale-up
The ability to scale up in liquid or AGT dry format provides ease of handling and storage in manufacturing and other bioprocess settings. Fewer parts and steps in reconstitution mean fewer opportunities for operator error. Further, the ability to store this feed, even when super-concentrated, for the length of a bioreactor run at room temperature, in the dark, helps reduce chances of contamination during closed system connections by reducing the number of connections to the bioreactor to one.