Shop All Williams' Media E

William's E Medium, no phenol red Gibco™

William's E Medium was originally developed by Williams and Gunn as reduced serum-supplemented medium for long-term cell cultures of adult rat liver epithelial cells. Gibco® William's E Medium can also be used for growing hepatocyte cells of different species, such as human derived HepaRG™ cells.
This William's E Medium is modified as follows:
Without
• Phenol Red
• L-glutamine
• HEPES

The complete formulation is available.

Gibco® William's E Medium is a modification of earlier media that has been enriched in amino acids and double the glucose. William's E Medium contains unique ingredients, including zinc, iron, manganese, non-essential amino acids, the reducing agent glutathione and the lipid methyl linoleate

Dual-Site cGMP Manufacturing
For supply chain continuity, we manufacture Gibco® William's E Medium at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.

William's E Medium contains no proteins or growth factors. Therefore, William's E Medium requires supplementation, commonly with 5-10% Fetal Bovine Serum (FBS). William's E Medium uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

William's E Medium, GlutaMAX™ Supplement Gibco™

William's E Medium was originally developed by Williams and Gunn as reduced serum-supplemented medium for long-term cell cultures of adult rat liver epithelial cells. Gibco® William's E Medium can also be used for growing hepatocyte cells of different species, such as human derived HepaRG™ cells.
This William's E Medium is modified as follows:
WithWithout
• Phenol Red• HEPES
• GlutaMAX™

The complete formulation is available.

Gibco® William's E Medium is a modification of earlier media that has been enriched in amino acids and double the glucose. William's E Medium contains unique ingredients, including zinc, iron, manganese, non-essential amino acids, the reducing agent glutathione and the lipid methyl linoleate. Gibco® William's E Medium with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.

cGMP Manufacturing
Gibco® William's E Medium is manufactured at a cGMP compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

William's E Medium contains no proteins or growth factors. Therefore, William's E Medium requires supplementation, commonly with 5-10% Fetal Bovine Serum (FBS). William's E Medium uses a sodium bicarbonate buffer system (2.2 g / L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

William's E Medium, no glutamine Gibco™

William's E Medium was originally developed by Williams and Gunn as reduced serum-supplemented medium for long-term cell cultures of adult rat liver epithelial cells. Gibco® William's E Medium can also be used for growing hepatocyte cells of different species, such as human derived HepaRG™ cells.
This William's E Medium is modified as follows:
WithWithout
• Phenol Red• L-glutamine
• HEPES

The complete formulation is available.

Gibco® William's E Medium is a modification of earlier media that has been enriched in amino acids and double the glucose. William's E Medium contains unique ingredients, including zinc, iron, manganese, non-essential amino acids, the reducing agent glutathione and the lipid methyl linoleate.

Dual-Site cGMP Manufacturing
Gibco® William's E Medium is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer a comparable Gibco® William's E Medium product made in our Grand Island facility (12551-032). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

William's E Medium contains no proteins or growth factors. Therefore, William's E Medium requires supplementation, commonly with 5-10% Fetal Bovine Serum (FBS). William's E Medium uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Hepatocyte Wash Medium Gibco™

Hepatocyte Wash Medium is an enriched Williams' Medium E for the reduction of cell membrane leakage, ease of centrifugation and high viability.
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