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Pierce™ Protein Concentrator PES, 10K MWCO, 2-6 mL (Thermo Scientific™)

Thermo Scientific™ Pierce™ Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for concentration, desalting, and buffer exchange of biological samples. This product has a molecular weight cutoff (MWCO) of 10K and can be used for processing sample volumes ranging from 2 to 6 mL.

Features of Pierce Protein Concentrators PES:

• Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 5 to 30 minutes for 10K MWCO (time may vary for a product with a different MWCO)
• Excellent protein recovery—typical protein recovery is >90%
• Versatility—perform sample concentration, diafiltration, or buffer-exchange of biological samples
• Compatibility—may be used in both fixed angle and swing bucker rotors; collect and recover sample without invert spinning

For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low nonspecific binding, and negligible protein aggregation on the membrane; and it offers reliable and consistent results.

Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least 2-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration. To achieve >90% recovery of protein, the minimum protein sample concentration should be 0.1 mg/mL.

cDNA Size Fractionation Columns (Invitrogen™)

cDNA Size Fractionation Columns offer an efficient and convenient method for size-fractioning double-stranded cDNA (1).

• Pre-packed, disposable columns efficiently remove small (<500 bp) cDNAs-including residual adapters, linkers, or other fragments that could interfere with ligation of cDNA to the vector
• Columns size-fractionate cDNA >500 bp

Performance and Quality Testing: Wash time, drop volume during elution of fragments, and the ability to clearly and cleanly separate a mixture of dyes, including xylene cyanol and bromophenol blue, are evaluated.

Pierce™ Microcentrifuge Tubes, 1.5 mL (Thermo Scientific™)

Thermo Scientific Pierce 1.5 mL Capacity Microcentrifuge Tubes are convenient tools for manipulating small volumes of affinity supports for protein purification.

Features of 1.5 mL Capacity Microcentrifuge Tubes:

• 1.5 mL capacity
• Polypropylene formulation
• Attached snap caps

Simply add the affinity resin and sample to one of the microcentrifuge tubes, then use a microcentrifuge to efficiently wash away contaminants and elute your purified sample without losing any resin in the process. Microcentrifuge tubes allow you to affinity purify more protein in less time.

Applications:
• Affinity purification or affinity chromatography
• Immunodepletion
• Immunoprecipitation (IP)
• Co-immunoprecipitation (co-IP)

Related Products
Pierce™ Microcentrifuge Tubes, 2.0 mL

Pierce™ Protein Concentrator PES, 30K MWCO, 5-20 mL (Thermo Scientific™)

Thermo Scientific™ Pierce™ Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for concentration, desalting, and buffer exchange of biological samples. This product has a molecular weight cutoff (MWCO) of 30K and can be used for processing sample volumes ranging from 5 to 20 mL.

Features of Pierce Protein Concentrators PES:

• Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 5 to 30 minutes for 10K MWCO (time may vary for a product with a different MWCO)
• Excellent protein recovery—typical protein recovery is >90%
• Versatility—perform sample concentration, diafiltration, or buffer-exchange of biological samples
• Compatibility—may be used in both fixed angle and swing bucker rotors; collect and recover sample without invert spinning

For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low nonspecific binding, and negligible protein aggregation on the membrane; and it offers reliable and consistent results.

Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least 2-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration. To achieve >90% recovery of protein, the minimum protein sample concentration should be 0.1 mg/mL.

Pierce™ Protein Concentrator PES, 5K MWCO, 20-100 mL (Thermo Scientific™)

Thermo Scientific™ Pierce™ Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for concentration, desalting, and buffer exchange of biological samples. This product has a molecular weight cutoff (MWCO) of 5K and can be used for processing sample volumes ranging from 20 to 100 mL.

Features of Pierce Protein Concentrators PES:

• Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 5 to 30 minutes for 10K MWCO (time may vary for a product with a different MWCO)
• Excellent protein recovery—typical protein recovery is >90%
• Versatility—perform sample concentration, diafiltration, or buffer-exchange of biological samples
• Compatibility—may be used in both fixed angle and swing bucker rotors; collect and recover sample without invert spinning

For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low nonspecific binding, and negligible protein aggregation on the membrane; and it offers reliable and consistent results.

Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least 2-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration. To achieve >90% recovery of protein, the minimum protein sample concentration should be 0.1 mg/mL.

Pierce™ Microcentrifuge Tubes, 2.0 mL (Thermo Scientific™)

Thermo Scientific Pierce 2 mL Capacity Microcentrifuge Tubes are convenient tools for manipulating small volumes of affinity supports for protein purification.

Features of 2 mL Capacity Microcentrifuge Tubes:

• 1.5 mL capacity
• Polypropylene formulation
• Attached snap caps

Simply add the affinity resin and sample to one of the microcentrifuge tubes, then use a microcentrifuge to efficiently wash away contaminants and elute your purified sample without losing any resin in the process. Microcentrifuge tubes allow you to affinity purify more protein in less time.

Applications:
• Affinity purification or affinity chromatography
• Immunodepletion
• Immunoprecipitation (IP)
• Co-immunoprecipitation (co-IP)

Related Products
Pierce™ Microcentrifuge Tubes, 1.5 mL

Pierce™ Protein Concentrator PES, 30K MWCO, 2-6 mL (Thermo Scientific™)

Thermo Scientific™ Pierce™ Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for concentration, desalting, and buffer exchange of biological samples. This product has a molecular weight cutoff (MWCO) of 30K and can be used for processing sample volumes ranging from 2 to 6 mL.

Features of Pierce Protein Concentrators PES:

• Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 5 to 30 minutes for 10K MWCO (time may vary for a product with a different MWCO)
• Excellent protein recovery—typical protein recovery is >90%
• Versatility—perform sample concentration, diafiltration, or buffer-exchange of biological samples
• Compatibility—may be used in both fixed angle and swing bucker rotors; collect and recover sample without invert spinning

For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low nonspecific binding, and negligible protein aggregation on the membrane; and it offers reliable and consistent results.

Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least 2-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration. To achieve >90% recovery of protein, the minimum protein sample concentration should be 0.1 mg/mL.

Pierce™ Spin Columns - Snap Cap (Thermo Scientific™)

Thermo Scientific Pierce Snap Cap Spin Columns are convenient tools for manipulating small volumes of affinity supports (20 to 500 µL) for protein purification.

Features of Snap Cap Spin Columns:

Formulation: polypropylene
Column volume: 1 mL
Resin volume: 20 to 500µL
Filter type: Polyethylene filter, ~30 µm pore size
Cap: Attached snap-cap (no cap on collection tube); press-on bottom caps

Simply add the affinity resin and sample to one of the spin columns, then use a microcentrifuge to efficiently wash away contaminants and elute your purified sample without losing any resin in the process. Spin columns allow you to affinity purify more protein in less time.

Applications:
• Affinity purification or affinity chromatography
• Immunodepletion
• Immunoprecipitation (IP)
• Co-immunoprecipitation (co-IP)

Related Products
Pierce™ Spin Columns - Screw Cap
Pierce™ Spin Cups - Paper Filter
Pierce™ Spin Cups - Cellulose Acetate Filter
Pierce™ Micro-Spin Columns

Pierce™ Protein Concentrator PES, 10K MWCO, 20-100 mL (Thermo Scientific™)

Thermo Scientific™ Pierce™ Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for concentration, desalting, and buffer exchange of biological samples. This product has a molecular weight cutoff (MWCO) of 10K and can be used for processing sample volumes ranging from 20 to 100 mL.

Features of Pierce Protein Concentrators PES:

• Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 5 to 30 minutes for 10K MWCO (time may vary for a product with a different MWCO)
• Excellent protein recovery—typical protein recovery is >90%
• Versatility—perform sample concentration, diafiltration, or buffer-exchange of biological samples
• Compatibility—may be used in both fixed angle and swing bucker rotors; collect and recover sample without invert spinning

For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low nonspecific binding, and negligible protein aggregation on the membrane; and it offers reliable and consistent results.

Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least 2-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration. To achieve >90% recovery of protein, the minimum protein sample concentration should be 0.1 mg/mL.

POROS™ GoPure™ HS Pre-packed Column, 0.8 x 10 cm, 5 mL (Thermo Scientific™)

GoPure 0.8 cmD pre-packed columns are intended for the screening of POROS affinity, ion exchange, and hydrophobic interaction chromatography (HIC) resins or for small scale purification. These pre-packed columns are ready to connect and have the following features:

• Dimensions of 0.8 cmD x 10 cmL, with a nominal bed volume of 5 mL, providing a uniform screening platform
• Direct connection to standard chromatography systems
>• Convenient—ready-to-run columns simplify and speed POROS resin evaluations
• The 10-cm bed height and 5-mL bed volume allow initial process development at a bench scale
• Packed with POROS high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates

The POROS HS 50 µm resin in this GoPure column has a sulphopropyl functional group for strong cation exchange chromatography.

Other POROS anion exchange resins, as well cation exchange resins, are also available in this column format. All POROS High Performance Chromatography resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.

Pierce™ Dye and Biotin Removal Spin Columns, 10 mL (Thermo Scientific™)

The Thermo Scientific Pierce Dye and Biotin Removal columns enable fast and efficient removal of non-reacted fluorescent dyes, biotin, reducing agents, and crosslinkers from protein samples. Removing free-dye after a labeling reaction is often difficult and time-consuming but is essential for accurate determination of dye-to-protein ratios. The Pierce Dye and Biotin Removal resin is highly specialized to produce exceptional protein recoveries while effectively removing non-conjugated fluorescent dyes, biotinylation reagents, reducing agents, and crosslinkers. Using the appropriate amount of sample and buffer conditions, almost any fluorescent dye can be removed with these columns. Even dilute protein samples (25 µg/mL) can be successfully processed to remove dyes, biotin, reducing agents, and crosslinkers and to efficiently recover proteins (>7 kDa). A single 10-mL Dye and Biotin Removal Spin Column can process a 2 to 4 mL of sample. This product is not recommended for desalting or buffer exchange.

Features of Pierce Dye and Biotin Removal columns:
Versatile—removes unreacted fluorescent dyes, biotinylation reagents, crosslinkers, and reducing agents from protein solutions
High recovery—low-binding resin maximizes protein recovery
Easy-to-use—no cumbersome column preparation or equilibration
Fast—small-molecule removal and protein recovery in less than 15 mins
Flexible—available in spin columns and filter spin plates for a range of needs

Available formats:
• Spin columns: 0.5, 2, 5, and 10 mL
• 96-well filter plates: pre-dispensed; compatible with centrifugation for manual or automated purification; enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput clean-ups

Applications:
• Ideal for post-reaction clean-up of samples
• To effectively remove non-conjugated dye, biotin, reducing agents, and crosslinkers with exceptional protein recovery

Pierce™ Dye and Biotin Removal Spin Columns, 2 mL (Thermo Scientific™)

The Thermo Scientific Pierce Dye and Biotin Removal columns enable fast and efficient removal of non-reacted fluorescent dyes, biotin, reducing agents, and crosslinkers from protein samples. Removing free-dye after a labeling reaction is often difficult and time-consuming but is essential for accurate determination of dye-to-protein ratios. The Pierce Dye and Biotin Removal resin is highly specialized to produce exceptional protein recoveries while effectively removing non-conjugated fluorescent dyes, biotinylation reagents, reducing agents, and crosslinkers. Using the appropriate amount of sample and buffer conditions, almost any fluorescent dye can be removed with these columns. Even dilute protein samples (25 µg/mL) can be successfully processed to remove dyes, biotin, reducing agents, and crosslinkers and to efficiently recover proteins (>7 kDa). A single 2-mL Dye and Biotin Removal Spin Column can process a 400 to 700 µL of sample. This product is not recommended for desalting or for buffer exchange

Features of Pierce Dye and Biotin Removal columns:
Versatile—removes unreacted fluorescent dyes, biotinylation reagents, crosslinkers, and reducing agents from protein solutions
High recovery—low-binding resin maximizes protein recovery
Easy-to-use—no cumbersome column preparation or equilibration
Fast—small-molecule removal and protein recovery in less than 15 mins
Flexible—available in spin columns and filter spin plates for a range of needs

Available formats:
• Spin columns: 0.5, 2, 5, and 10 mL
• 96-well filter plates: pre-dispensed; compatible with centrifugation for manual or automated purification; enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput clean-ups

Applications:
• Ideal for post-reaction clean-up of samples
• To effectively remove non-conjugated dye, biotin, reducing agents, and crosslinkers with exceptional protein recovery

Pierce™ Protein Concentrators PES, 50K MWCO, 20–100 mL (Thermo Scientific™)

Thermo Scientific Pierce Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for concentration, desalting, and buffer exchange of biological samples. This product has a molecular weight cutoff (MWCO) of 50K and can be used for processing sample volumes ranging from 20 to 100 mL.

Features of Pierce Protein Concentrators PES:
Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 5 to 30 minutes for 50K MWCO (time may vary for a product with a different MWCO)
Excellent protein recovery—typical protein recovery is >90%
Versatility—perform sample concentration, diafiltration, or buffer-exchange of biological samples
Compatibility—may be used in both fixed angle and swing bucker rotors; collect and recover sample without invert spinning

For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low nonspecific binding, and negligible protein aggregation on the membrane; and it offers reliable and consistent results.

Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least 2-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration. To achieve >90% recovery of protein, the minimum protein sample concentration should be 0.1 mg/mL.

Centri-Sep™ 8-Well Strips (Applied Biosystems™)

The Centri-Sep™ strips are provided in a convenient pre-hydrated 8-well strip format that allows you to use as many 8-well strips as needed. In addition to removing excess BigDye® terminators, Centri-Sep™ 8 strips also work well for removing unwanted salts, low-molecular-weight impurities, free and labeled dNTP's from DNA and excess nucleotides and small primers from PCR reactions and for purification of proteins.

For Research Use Only. Not for use in diagnostics procedures.

POROS™ GoPure™ Ethyl Pre-packed Column, 0.8 x 10 cm, 5 mL (Thermo Scientific™)

POROS GoPure 0.8 cmD pre-packed columns are intended for the screening of POROS affinity, ion exchange, and hydrophobic interaction chromatography (HIC) and hydrophobic interaction chromatography (HIC) resins or for small-scale purification. These pre-packed columns are ready to connect and have the following features:

• Dimensions of 0.8 cmD x 10 cmL, with a nominal bed volume of 5 mL, providing a uniform screening platform
• Direct connection to standard chromatography systems
>• Convenient—ready-to-run columns simplify and speed POROS resin evaluations
• The 10-cm bed height and 5-mL bed volume allow initial process development at a bench scale
• Packed with POROS high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates

The POROS Ethyl HIC resin in this GoPure column has a novel hydrophobic ethyl ligand, making it suited primarily for bind/elute purifications.

Other POROS hydrophobic interaction and ion exchange resins are also available in this column format. All POROS High Performance Chromatography resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.