Shop All Spin Filters, Columns, & Purification Plates

PrepFiler™ LySep™ Columns (Applied Biosystems™)

PrepFiler LySep Columns are intended for use with the PrepFiler Forensic DNA Extraction kits and allow users to easily perform lysis and substrate removal in a single tube assembly. There is no transfer of the substrate to another tube for removal, thus minimizing hands-on time and helping to reduce the risk of cross contamination and sample transposition events. The only analyst interaction and handling of the substrate occurs during the addition of the substrate to the PrepFiler LySep Column for lysis.

The innovative PrepFiler LySep Column dramatically streamlines the off-line lysis portion of the extraction method. PrepFiler Lysis Buffer or PrepFiler BTA Lysis buffer is added directly to the PrepFiler LySep Column along with the sample for incubation. Next, the PrepFiler LySep Column is briefly centrifuged, causing the lysate to flow through the burstable membrane while the substrate remains behind in the column. The sample tube is then ready to be placed on the AutoMate Express instrument. This eliminates the manual lysate and substrate transfer steps, helping save time and minimizing cross contamination and sample transposition events.

Pierce™ Spin Cups - Cellulose Acetate Filter (Thermo Scientific™)

Thermo Scientific Pierce Cellulose Acetate Filter Spin Cups are convenient tools for manipulating small volumes of affinity supports (20 to 400 µL) for protein purification.

Features of Cellulose Acetate Filter Spin Cups:

Smaller pore size: cellulose acetate filters have smaller pore sizes than paper
Column volume: 800µL
Resin volume: 20 to 400µL
Filter type: cellulose acetate filter, 0.45µm pore size
Cap: collection tube cap fits onto inserted spin cup

Simply add the affinity resin and sample to one of the spin cups, then use a microcentrifuge to efficiently wash away contaminants and elute your purified sample without losing any resin in the process. Spin cups allow you to affinity purify more protein in less time.

Applications:
• Affinity purification or affinity chromatography
• Immunodepletion
• Immunoprecipitation (IP)
• Co-immunoprecipitation (co-IP)

Related Products
Pierce™ Spin Columns - Screw Cap
Pierce™ Spin Cups - Paper Filter
Pierce™ Spin Columns - Snap Cap
Pierce™ Micro-Spin Columns

PrepFiler™ 96-Well Spin Plates and Filter Plates, ethylene oxide-treated (Applied Biosystems™)

PrepFiler 96-Well Spin Plates and Filter Plates are intended for use with the updated PrepFiler Automated Forensic DNA Extraction Kit, which does not include plastic consumables, providing users the flexibility to order only the plastics consumables they need. PrepFiler Forensic DNA Extraction kits use magnetic particles with an optimized multi-component surface chemistry to deliver superior DNA yield from most forensic sample types, including body fluids, and stains and swabs of body fluids. The PrepFiler Spin Plates and Filter Plates are used for sample lysis and substrate removal for sample processing in 96-well plate format. The packaged plates are treated with ethylene oxide.

Pierce™ Protein Concentrators PES, 50K MWCO, 5–20 mL (Thermo Scientific™)

Thermo Scientific Pierce Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for concentration, desalting, and buffer exchange of biological samples. This product has a molecular weight cutoff (MWCO) of 50K and can be used for processing sample volumes ranging from 5 to 20 mL.

Features of Pierce Protein Concentrators PES:
Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 5 to 30 minutes for 10K MWCO (time may vary for a product with a different MWCO)
Excellent protein recovery—typical protein recovery is >90%
Versatility—perform sample concentration, diafiltration, or buffer-exchange of biological samples
Compatibility—may be used in both fixed angle and swing bucker rotors; collect and recover sample without invert spinning

For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low nonspecific binding, and negligible protein aggregation on the membrane; and it offers reliable and consistent results.

Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least 2-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration. To achieve >90% recovery of protein, the minimum protein sample concentration should be 0.1 mg/mL.

Centri-Sep™ 96-Well Plates (Applied Biosystems™)

Centri-Sep™ 96's hydrated matrix enables effective and reliable high-throughput removal of excess BigDye® terminators from DNA sequencing reactions, giving you clean sequencing reads with uniform signal intensity starting with the first base. The plate's 96-well format enables you to purify 96 samples simultaneously, either manually or by robotics, within 15 minutes.

• Processed faster than ultrafiltration columns
• Rapid and efficient separations
• Buffer is not preselected
• Convenient 20-100 µL sample size
• Dry gel format
• Room Temperature Storage
• Microcentrifuge format

Designed for Performance
The column design is based on the description of gel filtration for the purification of DNA from nick translation reactions by Sambrook, et.al. Each Centri-Sep unit consists of a specially fritted microfuge tube, dry gel, a wash tube and a sample collection tube, all specifically designed for this purpose.

Excellent Recovery
The Centri-Sep™ gel will provide excellent recovery of DNA fragments with sizes greater than 16 base pairs or 25-mer, while removing 98% of salts, NTP's and other unwanted low molecular-weight impurities.

Versatile
The column gel is hydrated with either reagent-grade water or a suitable buffer or your choice, and spun in a microcentrifuge or swinging-bucket centrifuge to remove the intersititial fluid. Your sample is then applied to the column and spun again, leaving you not only with a sample purified through removal of low-molecular-weight components, but also exchanged into the buffer of your choice.

For Research Use Only. Not for use in diagnostics procedures.

Pierce™ Spin Columns - Screw Cap (Thermo Scientific™)

Thermo Scientific Pierce Screw Cap Spin Columns are convenient tools for manipulating small volumes of affinity supports (20 to 400 µL) for protein purification.

Features of Screw Cap Spin Columns:

Adaptable: included Luer-Lok adaptors allow these columns to be used for syringe-based purifications
Column volume: 900µL
Resin volume: 20 to 400µL
Filter type: polyethylene filter, ~10µm pore size
Cap: O-ring screw-top caps
Frit options: included small & large frit options for different sample sizes

Simply add the affinity resin and sample to one of the spin columns, then use a microcentrifuge to efficiently wash away contaminants and elute your purified sample without losing any resin in the process. Spin columns allow you to affinity purify more protein in less time.

Applications:
• Affinity purification or affinity chromatography
• Immunodepletion
• Immunoprecipitation (IP)
• Co-immunoprecipitation (co-IP)

Related Products
Pierce™ Spin Cups - Paper Filter
Pierce™ Spin Cups - Cellulose Acetate Filter
Pierce™ Spin Columns - Snap Cap
Pierce™ Micro-Spin Columns

POROS™ GoPure™ Benzyl Pre-packed Column, 0.8 x 10 cm, 5 mL (Thermo Scientific™)

POROS GoPure 0.8 cmD pre-packed columns are intended for the screening of POROS affinity, ion exchange, and hydrophobic interaction chromatography (HIC) and hydrophobic interaction chromatography (HIC) resins or for small-scale purification. These pre-packed columns are ready to connect and have the following features:

• Dimensions of 0.8 cmD x 10 cmL, with a nominal bed volume of 5 mL, providing a uniform screening platform
• Direct connection to standard chromatography systems
>• Convenient—ready-to-run columns simplify and speed POROS resin evaluations
• The 10-cm bed height and 5-mL bed volume allow initial process development at a bench scale
• Packed with POROS high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates

The POROS Benzyl HIC resin in this GoPure column has an aromatic hydrophobic benzyl ligand, suited for hydrophobic interaction chromatography (HIC) for both bind/elute and flow-through purifications.

Other POROS hydrophobic interaction and ion exchange resins are also available in this column format. All POROS High Performance Chromatography resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.

Pierce™ Microcentrifuge Tubes, 2.0 mL (Thermo Scientific™)

Thermo Scientific Pierce 2 mL Capacity Microcentrifuge Tubes are convenient tools for manipulating small volumes of affinity supports for protein purification.

Features of 2 mL Capacity Microcentrifuge Tubes:

• 1.5 mL capacity
• Polypropylene formulation
• Attached snap caps

Simply add the affinity resin and sample to one of the microcentrifuge tubes, then use a microcentrifuge to efficiently wash away contaminants and elute your purified sample without losing any resin in the process. Microcentrifuge tubes allow you to affinity purify more protein in less time.

Applications:
• Affinity purification or affinity chromatography
• Immunodepletion
• Immunoprecipitation (IP)
• Co-immunoprecipitation (co-IP)

Related Products
Pierce™ Microcentrifuge Tubes, 1.5 mL

Pierce™ Protein Concentrators PES, 50K MWCO, 20–100 mL (Thermo Scientific™)

Thermo Scientific Pierce Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for concentration, desalting, and buffer exchange of biological samples. This product has a molecular weight cutoff (MWCO) of 50K and can be used for processing sample volumes ranging from 20 to 100 mL.

Features of Pierce Protein Concentrators PES:
Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 5 to 30 minutes for 50K MWCO (time may vary for a product with a different MWCO)
Excellent protein recovery—typical protein recovery is >90%
Versatility—perform sample concentration, diafiltration, or buffer-exchange of biological samples
Compatibility—may be used in both fixed angle and swing bucker rotors; collect and recover sample without invert spinning

For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low nonspecific binding, and negligible protein aggregation on the membrane; and it offers reliable and consistent results.

Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least 2-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration. To achieve >90% recovery of protein, the minimum protein sample concentration should be 0.1 mg/mL.

Pierce™ Protein Concentrator PES, 100K MWCO, 20-100 mL (Thermo Scientific™)

Thermo Scientific™ Pierce™ Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for concentration, desalting, and buffer exchange of biological samples. This product has a molecular weight cutoff (MWCO) of 100K and can be used for processing sample volumes ranging from 20 to 100 mL.

Features of Pierce Protein Concentrators PES:

• Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 5 to 30 minutes for 10K MWCO (time may vary for a product with a different MWCO)
• Excellent protein recovery—typical protein recovery is >90%
• Versatility—perform sample concentration, diafiltration, or buffer-exchange of biological samples
• Compatibility—may be used in both fixed angle and swing bucker rotors; collect and recover sample without invert spinning

For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low nonspecific binding, and negligible protein aggregation on the membrane; and it offers reliable and consistent results.

Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least 2-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration. To achieve >90% recovery of protein, the minimum protein sample concentration should be 0.1 mg/mL.

Chargeswitch™ Pro Filter Collection Tube (Invitrogen™)

The Chargeswitch® -Pro Filter Collection Tube is a 50 ml conical tube that will accommodate the centrifuge protocol of Chargeswitch® Pro Filter Plasmid Midi and Maxi kits

Polypropylene Columns (Empty) (Invitrogen™)

These empty, 10-mL polypropylene columns are for use with the ProBond™ Purification System and hold ProBond™ resin.

Centri-Sep™ 8-Well Strips (Applied Biosystems™)

The Centri-Sep™ strips are provided in a convenient pre-hydrated 8-well strip format that allows you to use as many 8-well strips as needed. In addition to removing excess BigDye® terminators, Centri-Sep™ 8 strips also work well for removing unwanted salts, low-molecular-weight impurities, free and labeled dNTP's from DNA and excess nucleotides and small primers from PCR reactions and for purification of proteins.

For Research Use Only. Not for use in diagnostics procedures.

Pierce™ Protein Concentrator PES, 30K MWCO, 5-20 mL (Thermo Scientific™)

Thermo Scientific™ Pierce™ Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for concentration, desalting, and buffer exchange of biological samples. This product has a molecular weight cutoff (MWCO) of 30K and can be used for processing sample volumes ranging from 5 to 20 mL.

Features of Pierce Protein Concentrators PES:

• Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 5 to 30 minutes for 10K MWCO (time may vary for a product with a different MWCO)
• Excellent protein recovery—typical protein recovery is >90%
• Versatility—perform sample concentration, diafiltration, or buffer-exchange of biological samples
• Compatibility—may be used in both fixed angle and swing bucker rotors; collect and recover sample without invert spinning

For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low nonspecific binding, and negligible protein aggregation on the membrane; and it offers reliable and consistent results.

Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least 2-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration. To achieve >90% recovery of protein, the minimum protein sample concentration should be 0.1 mg/mL.

POROS™ GoPure™ Benzyl Ultra Pre-packed Column, 0.8 x 10 cm, 5 mL (Thermo Scientific™)

POROS GoPure 0.8 cmD pre-packed columns are intended for the screening of POROS affinity, ion exchange, and hydrophobic interaction chromatography (HIC) and hydrophobic interaction chromatography (HIC) resins or for small-scale purification. These pre-packed columns are ready to connect and have the following features:

• Dimensions of 0.8 cmD x 10 cmL, with a nominal bed volume of 5 mL, providing a uniform screening platform
• Direct connection to standard chromatography systems
>• Convenient—ready-to-run columns simplify and speed POROS resin evaluations
• The 10-cm bed height and 5-mL bed volume allow initial process development at a bench scale
• Packed with POROS high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates

The POROS Benzyl Ultra HIC resin in this GoPure column has an aromatic hydrophobic benzyl ligand, well suited for hydrophobic interaction chromatography (HIC) for aggregate removal from monoclonal antibody monomer in flow-through mode at low salt concentrations.

Other POROS hydrophobic interaction and ion exchange resins are also available in this column format. All POROS High Performance Chromatography resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.