Shop All Spin Filters, Columns, & Purification Plates

PrepFiler™ LySep™ Columns (Applied Biosystems™)

PrepFiler® LySep™ Columns are intended for use with the PrepFiler® Forensic DNA Extraction kits and allow users to easily perform lysis and substrate removal in a single tube assembly. There is no transfer of the substrate to another tube for removal, thus minimizing hands-on time and helping to reduce the risk of cross contamination and sample transposition events. The only analyst interaction and handling of the substrate occurs during the addition of the substrate to the PrepFiler LySep™ Column for lysis.

The innovative PrepFiler® LySep™ Column dramatically streamlines the off-line lysis portion of the extraction method. PrepFiler® Lysis Buffer or PrepFiler® BTA™ Lysis buffer is added directly to the PrepFiler® LySep™ Column along with the sample for incubation. Next, the PrepFiler® LySep™ Column is briefly centrifuged, causing the lysate to flow through the burstable membrane while the substrate remains behind in the column. The sample tube is then ready to be placed on the AutoMate Express™ instrument. This eliminates the manual lysate and substrate transfer steps, helping save time and minimizing cross contamination and sample transposition events.

Pierce™ Protein Concentrator PES, 30K MWCO, 5-20 mL (Thermo Scientific™)

Thermo Scientific™ Pierce™ Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for concentration, desalting, and buffer exchange of biological samples. This product has a molecular weight cutoff (MWCO) of 30K and can be used for processing sample volumes ranging from 5 to 20 mL.

Features of Pierce Protein Concentrators PES:

• Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 5 to 30 minutes for 10K MWCO (time may vary for a product with a different MWCO)
• Excellent protein recovery—typical protein recovery is >90%
• Versatility—perform sample concentration, diafiltration, or buffer-exchange of biological samples
• Compatibility—may be used in both fixed angle and swing bucker rotors; collect and recover sample without invert spinning

For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low nonspecific binding, and negligible protein aggregation on the membrane; and it offers reliable and consistent results.

Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least 2-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration. To achieve >90% recovery of protein, the minimum protein sample concentration should be 0.1 mg/mL.

Pierce™ Protein Concentrators PES, 3K MWCO, 0.5 mL (Thermo Scientific™)

Thermo Scientific Pierce Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for concentration, desalting and buffer exchange of biological samples. This product has a molecular-weight cutoff of 3K, and can be used for processing sample volumes up to 0.5 mL.

Features of Pierce Protein Concentrators PES:

Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 10 minutes or less
Excellent protein recovery—unique design minimizes membrane fouling and provides high speed concentrations, even with particle laden solutions
Instrument-compatible—use standard microcentrifuge with fixed-angle rotors; collect and recover sample without invert spinning
Versatile—easy way to perform sample concentration, diafiltration or buffer-exchange of biological samples

For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low non-specific binding and negligible protein aggregation on the membrane and provides reliable and consistent results for processing sample volumes between 100 and 500 µL. Typical protein recovery is 90% when the mass of the proteins in the sample is at least two-fold greater than the MWCO of the device used. Sample concentration of 10- to 30-fold can be achieved within 10 minutes.

Applications:
• Protein concentration with tissue culture media, antiserum or monoclonal antibody preparations
• Concentration of protein peaks following gel-permeation chromatography
• Removal of unincorporated label following protein modification reactions
• Concentration and desalt/buffer-exchange after eluting protein from ion-exchange, hydrophobic interaction (HIC), metal chelate or affinity chromatography columns
• Separating large molecules from small molecules

Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least two-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration. To achieve >90% recovery of protein, the minimum protein sample concentration should be 0.05 mg/mL.

Pierce Protein Concentrators PES allow accurate control of the dead-stop volume (15 µL) and final concentration factor, providing reliable and consistent sample processing. They can be used effectively at a maximum centrifugal force of 15,000 x g at room temperature. Centrifugal force, temperature and sample volume, concentration and viscosity affect filtration rate. Optimize centrifugal time for each application.

Related Products
Pierce™ Protein Concentrators PES, 10K MWCO, 0.5 mL
Pierce™ Protein Concentrators PES, 30K MWCO, 0.5 mL
Pierce™ Protein Concentrators PES, 100K MWCO, 0.5 mL

Pierce™ Protein Concentrator PES, 10K MWCO, 5-20 mL (Thermo Scientific™)

Thermo Scientific™ Pierce™ Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for concentration, desalting, and buffer exchange of biological samples. This product has a molecular weight cutoff (MWCO) of 10K and can be used for processing sample volumes ranging from 5 to 20 mL.

Features of Pierce Protein Concentrators PES:

• Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 5 to 30 minutes for 10K MWCO (time may vary for a product with a different MWCO)
• Excellent protein recovery—typical protein recovery is >90%
• Versatility—perform sample concentration, diafiltration, or buffer-exchange of biological samples
• Compatibility—may be used in both fixed angle and swing bucker rotors; collect and recover sample without invert spinning

For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low nonspecific binding, and negligible protein aggregation on the membrane; and it offers reliable and consistent results.

Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least 2-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration. To achieve >90% recovery of protein, the minimum protein sample concentration should be 0.1 mg/mL.

Filter Cartridges & Tubes for cDNA Purification (Invitrogen™)

These filter cartridges and tubes are for use with the DNAclear™ cDNA Purification Kit.

The DNAclear™ cDNA Purification Kit is designed to purify double-stranded DNA (dsDNA) generated by enzymatic reactions such as cDNA synthesis and PCR.
• Efficiently purify and concentrate dsDNA ranging from 1 ng to 3 µg
• Ideal for downstream applications such as in vitro transcription
• Simple and fast protocol – about 10 minutes

The process is simple and fast. DNA amounts ranging from 1 ng to 3 µg can be efficiently purified and concentrated. The DNAclear™ procedure consists of three steps: 1) DNA is bound to the Microfilter Cartridge in the presence of the DNA Binding Buffer, 2) the DNA is washed with the cDNA Wash Buffer, and 3) the DNA is eluted with preheated Nuclease-free Water. The DNAclear™ Kit can be used to remove free nucleotides, oligonucleotides, proteins, and salts from dsDNA samples. The DNA that is recovered from the Microfilter Cartridge is ready for any application that requires highly pure DNA including in vitro transcription reactions. The DNAclear™ Kit includes enough materials to perform 20 purifications.

For Research Use Only. Not for use in diagnostics procedures.

Centri-Sep™ Spin Columns (Invitrogen™)

Centri-Sep™ Spin Columns are used for the fast and efficient purification of nucleic acids. The Centri-Sep™ gel provides excellent recovery of DNA fragments larger than 16 base pairs, while removing greater than 98% of salts, NTPs, and other low molecular weight impurities. Features of Centri-Sep™ columns:

• Processes samples faster than ultrafiltration columns
• Achieves rapid and efficient separation
• Permits use of custom buffers
• Convenient 20–100 µL sample size
• Dry gel format
• Room temperature storage
• Microcentrifuge format

Designed for performance
The column design is based on the description of gel filtration for the purification of DNA from nick translation reactions by Sambrook, et.al. Each Centri-Sep™ unit consists of a specially fritted microfuge tube, dry gel, a wash tube, and a sample collection tube, all specifically designed for this purpose.

Excellent recovery
The Centri-Sep™ gel provides excellent recovery of DNA fragments with sizes greater than 16 base pairs or a 25-mer, while removing >98% of salts, NTPs, and other unwanted low molecular weight impurities.

Versatile
The column gel is hydrated with either reagent-grade water or a buffer of your choice, and spun in a microcentrifuge or swinging-bucket centrifuge to remove the intersititial fluid. Your sample is then applied to the column and spun again, leaving you not only with a sample purified through removal of low molecular weight components, but also exchanged into the buffer of your choice.

Pierce™ Microcentrifuge Tubes, 2.0 mL (Thermo Scientific™)

Thermo Scientific Pierce 2 mL Capacity Microcentrifuge Tubes are convenient tools for manipulating small volumes of affinity supports for protein purification.

Features of 2 mL Capacity Microcentrifuge Tubes:

• 1.5 mL capacity
• Polypropylene formulation
• Attached snap caps

Simply add the affinity resin and sample to one of the microcentrifuge tubes, then use a microcentrifuge to efficiently wash away contaminants and elute your purified sample without losing any resin in the process. Microcentrifuge tubes allow you to affinity purify more protein in less time.

Applications:
• Affinity purification or affinity chromatography
• Immunodepletion
• Immunoprecipitation (IP)
• Co-immunoprecipitation (co-IP)

Related Products
Pierce™ Microcentrifuge Tubes, 1.5 mL

Pierce™ Protein Concentrators PES, 10K MWCO, 0.5 mL (Thermo Scientific™)

Thermo Scientific Pierce Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for concentration, desalting and buffer exchange of biological samples. This product has a molecular-weight cutoff of 10K, and can be used for processing sample volumes up to 0.5 mL.

Features of Pierce Protein Concentrators PES:

Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 10 minutes or less
Excellent protein recovery—unique design minimizes membrane fouling and provides high speed concentrations, even with particle laden solutions
Instrument-compatible—use standard microcentrifuge with fixed-angle rotors; collect and recover sample without invert spinning
Versatile—easy way to perform sample concentration, diafiltration or buffer-exchange of biological samples

For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low non-specific binding and negligible protein aggregation on the membrane and provides reliable and consistent results for processing sample volumes between 100 and 500 µL. Typical protein recovery is 90% when the mass of the proteins in the sample is at least two-fold greater than the MWCO of the device used. Sample concentration of 10- to 30-fold can be achieved within 10 minutes.

Applications:
• Protein concentration with tissue culture media, antiserum or monoclonal antibody preparations
• Concentration of protein peaks following gel-permeation chromatography
• Removal of unincorporated label following protein modification reactions
• Concentration and desalt/buffer-exchange after eluting protein from ion-exchange, hydrophobic interaction (HIC), metal chelate or affinity chromatography columns
• Separating large molecules from small molecules

Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least two-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration. To achieve >90% recovery of protein, the minimum protein sample concentration should be 0.05 mg/mL.

Pierce Protein Concentrators PES allow accurate control of the dead-stop volume (15 µL) and final concentration factor, providing reliable and consistent sample processing. They can be used effectively at a maximum centrifugal force of 15,000 x g at room temperature. Centrifugal force, temperature and sample volume, concentration and viscosity affect filtration rate. Optimize centrifugal time for each application.

Related Products
Pierce™ Protein Concentrators PES, 3K MWCO, 0.5 mL
Pierce™ Protein Concentrators PES, 30K MWCO, 0.5 mL
Pierce™ Protein Concentrators PES, 100K MWCO, 0.5 mL

NucAway™ Spin Columns (Invitrogen™)

Ambion® NucAway™ Spin Columns are for the fast removal of unincorporated nucleotides and salts from probes after probe synthesis reactions. 30 columns are provided.
• Fast, efficient removal of unincorporated nucleotides and salts after probe synthesis
• RNase- and DNase-free
• Replaces more time- consuming purification methods To use, simply rehydrate the column, centrifuge to remove the interstitial fluid, add the probe to the top of the column, and centrifuge again to elute the probe.

Pierce™ Centrifuge Columns, 0.8 mL (Thermo Scientific™)

Thermo Scientific Pierce Centrifuge Columns are convenient tools for handling 40 µL to 10 mL of an affinity support for purification.

Column details:

• Made from low protein binding polypropylene for compatibility with protein purification
• Total volume: 800 µL
• Resin volume: 40-400 µL
• Filter type, pore size: Polyethylene filter, ~30 µm pore size
• Receiver tube: Fits standard microcentrifuge tubes
• Caps: O-ring screw-top cap
• Twist-off bottom

Simply add the affinity resin to one of the polypropylene columns, remove the twist-off bottom and allow the resin to pack itself. Then add your sample and allow it to bind to the support and use a centrifuge to efficiently wash away any contaminants and elute your purified protein. Pierce Centrifuge Columns can be used in either spin or gravity-flow format. The spin format reduces sample processing time and makes column washing and elution more efficient. For gravity-flow procedures and when slow processing time is desired for binding steps with dilute samples, Column Extenders can be used to increase the column reservoir capacity. Use the any combination of processing techniques to get the best possible chromatography results.

Applications:

• Affinity purification, affinity chromatography
• Immunodepletion
• Ion exchange resins
• Gel filtration, desalting, buffer exchange

Related Products
Pierce™ Centrifuge Columns, 2 mL
Pierce™ Centrifuge Columns, 5 mL
Pierce™ Centrifuge Columns, 10 mL
Column Extenders for Pierce™ Centrifuge Columns

Pierce™ Protein Concentrators PES, 30K MWCO, 0.5 mL (Thermo Scientific™)

Thermo Scientific Pierce Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for concentration, desalting and buffer exchange of biological samples. This product has a molecular-weight cutoff of 30K, and can be used for processing sample volumes up to 0.5 mL.

Features of Pierce Protein Concentrators PES:

Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 10 minutes or less
Excellent protein recovery—unique design minimizes membrane fouling and provides high speed concentrations, even with particle laden solutions
Instrument-compatible—use standard microcentrifuge with fixed-angle rotors; collect and recover sample without invert spinning
Versatile—easy way to perform sample concentration, diafiltration or buffer-exchange of biological samples

For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low non-specific binding and negligible protein aggregation on the membrane and provides reliable and consistent results for processing sample volumes between 100 and 500 µL. Typical protein recovery is 90% when the mass of the proteins in the sample is at least two-fold greater than the MWCO of the device used. Sample concentration of 10- to 30-fold can be achieved within 10 minutes.

Applications:
• Protein concentration with tissue culture media, antiserum or monoclonal antibody preparations
• Concentration of protein peaks following gel-permeation chromatography
• Removal of unincorporated label following protein modification reactions
• Concentration and desalt/buffer-exchange after eluting protein from ion-exchange, hydrophobic interaction (HIC), metal chelate or affinity chromatography columns
• Separating large molecules from small molecules

Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least two-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration. To achieve >90% recovery of protein, the minimum protein sample concentration should be 0.05 mg/mL.

Pierce Protein Concentrators PES allow accurate control of the dead-stop volume (15 µL) and final concentration factor, providing reliable and consistent sample processing. They can be used effectively at a maximum centrifugal force of 15,000 x g at room temperature. Centrifugal force, temperature and sample volume, concentration and viscosity affect filtration rate. Optimize centrifugal time for each application.

Related Products
Pierce™ Protein Concentrators PES, 10K MWCO, 0.5 mL
Pierce™ Protein Concentrators PES, 3K MWCO, 0.5 mL
Pierce™ Protein Concentrators PES, 100K MWCO, 0.5 mL

PrepFiler™ 96-Well Spin Plates and Filter Plates, ethylene oxide-treated (Applied Biosystems™)

PrepFiler® 96-Well Spin Plates and Filter Plates are intended for use with the updated PrepFiler® Automated Forensic DNA Extraction Kit, which does not include plastic consumables, providing users the flexibility to order only the plastics consumables they need. PrepFiler® Forensic DNA Extraction kits use magnetic particles with an optimized multi-component surface chemistry to deliver superior DNA yield from most forensic sample types, including body fluids, and stains and swabs of body fluids. The PrepFiler® Spin Plates and Filter Plates are used for sample lysis and substrate removal for sample processing in 96-well plate format. The packaged plates are treated with ethylene oxide.

Pierce™ Centrifuge Columns, 2 mL (Thermo Scientific™)

Thermo Scientific Pierce Centrifuge Columns are convenient tools for handling 40 µL to 10 mL of an affinity support for purification.

Column details:

• Made from low protein binding polypropylene for compatibility with protein purification
• Total volume: 5 mL (2 mL resin bed, 3 mL reservoir)
• Resin volume: 2 mL
• Filter type, pore size: Polyethylene filter, ~30 µm pore size
• Receiver tube: Fits standard 15 mL conical centrifuge tubes
• Caps: Screw-top cap
• Twist-off bottom and press-on cap to reseal

Simply add the affinity resin to one of the polypropylene columns, remove the twist-off bottom and allow the resin to pack itself. Then add your sample and allow it to bind to the support and use a centrifuge to efficiently wash away any contaminants and elute your purified protein. Pierce Centrifuge Columns can be used in either spin or gravity-flow format. The spin format reduces sample processing time and makes column washing and elution more efficient. For gravity-flow procedures and when slow processing time is desired for binding steps with dilute samples, Column Extenders can be used to increase the column reservoir capacity. Use the any combination of processing techniques to get the best possible chromatography results.

Applications:

• Affinity purification, affinity chromatography
• Immunodepletion
• Ion exchange resins
• Gel filtration, desalting, buffer exchange

Related Products
Pierce™ Centrifuge Columns, 0.8 mL
Pierce™ Centrifuge Columns, 5 mL
Pierce™ Centrifuge Columns, 10 mL
Column Extenders for Pierce™ Centrifuge Columns

Chargeswitch™ Pro Filter Collection Tube (Invitrogen™)

The Chargeswitch® -Pro Filter Collection Tube is a 50 ml conical tube that will accommodate the centrifuge protocol of Chargeswitch® Pro Filter Plasmid Midi and Maxi kits

Pierce™ Centrifuge Columns, 10 mL (Thermo Scientific™)

Thermo Scientific Pierce Centrifuge Columns are convenient tools for handling 40 µL to 10 mL of an affinity support for purification.

Column details:

• Made from low protein binding polypropylene for compatibility with protein purification
• Total volume: 22 mL (10 mL resin bed, 12 mL reservoir)
• Resin volume: 10 mL
• Filter type, pore size: Polyethylene filter, ~30 µm pore size
• Receiver tube: Fits standard 50 mL conical centrifuge tubes
• Caps: Screw-top cap
• Twist-off bottom and press-on cap to reseal

Simply add the affinity resin to one of the polypropylene columns, remove the twist-off bottom and allow the resin to pack itself. Then add your sample and allow it to bind to the support and use a centrifuge to efficiently wash away any contaminants and elute your purified protein. Pierce Centrifuge Columns can be used in either spin or gravity-flow format. The spin format reduces sample processing time and makes column washing and elution more efficient. For gravity-flow procedures and when slow processing time is desired for binding steps with dilute samples, Column Extenders can be used to increase the column reservoir capacity. Use the any combination of processing techniques to get the best possible chromatography results.

Applications:

• Affinity purification, affinity chromatography
• Immunodepletion
• Ion exchange resins
• Gel filtration, desalting, buffer exchange

Related Products
Pierce™ Centrifuge Columns, 0.8 mL
Pierce™ Centrifuge Columns, 2 mL
Pierce™ Centrifuge Columns, 5 mL
Column Extenders for Pierce™ Centrifuge Columns