Shop All Blotting & Staining Systems

Mini Blot Module (Invitrogen™)

The Mini Blot Module is a wet transfer device used exclusively with the Mini Gel Tank. The tank accommodates one blot module per chamber, or two blot modules total with the side-by-side layout. The Mini Blot Module is compatible all Invitrogen mini gels (blot size: 8.5 cm x 8 cm). The blot module accommodates one mini gel per wet transfer. The universal connection and molded gasket make the module easy to use, while the inner core of the module allows for use of less methanol-based transfer buffer per wet transfer (250 mL per gel). At the recommended conditions and constant voltage, proteins can be transferred to nitrocellulose or PVDF membranes typically in 30 to 60 minutes.

See all available transfer systems ›

Power Blotter XL System (Invitrogen™)

The Invitrogen Power Blotter XL System is specifically designed for rapid semi-dry transfer of proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes in 5 to 12 minutes. The Power Blotter System includes an integrated power supply optimized to enable consistent and high-efficiency protein transfer.

Features of the Power Blotter XL System include:
Efficient—high transfer efficiency with a broad range of protein sizes compared to conventional semi-dry or wet (tank) transfer methods
Fast—5- to 12-minute transfer when used with Power Blotter Select Transfer Stacks or Power Blotter 1-Step Transfer Buffer
Integrated power supply—seamless operation between control unit and cassette; no need for external power supply
Easy-touch programming—convenient pre-programmed transfer methods or create, save, and run customized transfer methods
Flexible—simultaneous transfer of 1 to 4 mini-size or 1 to 2 midi-size gels with the Power Blotter Cassette XL
Versatile—compatible with Power Blotter Select Transfer Stacks and Pierce 1-Step Transfer Buffer for rapid blotting programs or Towbin transfer buffer for conventional semi-dry transfer methods

Learn more about the semi-dry transfer device: Power Blotter XL System

The Power Blotter XL Cassette, included in the Power Blotter XL System, enables the simultaneous transfer of up to four mini-size or two midi-size gels. The included Power Blotter Station has an easy-to-use color LCD touchscreen interface with pre-programmed transfer methods for different numbers and sizes of gels and ranges of molecular weight proteins. The easy-touch programming feature allows custom transfer settings to be quickly created, saved, and run.

The Power Blotter System achieves highly efficient transfer in seven minutes when used with Power Blotter Select Transfer Stacks or ten minutes with Power Blotter 1-Step Transfer Buffer. The instrument is also effective for standard 30- to 60-minute semi-dry transfer protocols based on traditional Towbin buffer.

Contents of the Power Blotter XL System:
Power Blotter Station
Power Blotter Cassette XL

Related products
Power Blotter System

Power Blotter Cassette (Invitrogen™)

The Invitrogen Power Blotter Cassette is part of the Power Blotter System designed specifically for rapid semi-dry transfer of proteins from polyacrylamide gels to nitrocellulose or PVDF membranes in 5 to 12 minutes. The Power Blotter Cassette can simultaneously transfer up to two mini-size or one midi-size gel. This item is made available as a replacement or additional cassette for the Power Blotter Station.

The Power Blotter Cassette can be used with Power Blotter Select Transfer Stacks for rapid transfers, the Power Blotter 1-Step Transfer Buffer for semi-dry transfers, or alternatively with traditional buffers for longer semi-dry transfers.

Learn more about the Power Blotter System ›

Related products
Power Blotter Cassette XL

XCell SureLock™ Mini-Cell and XCell II™ Blot Module (Invitrogen™)

The XCell SureLock Mini-Cell and XCell II Blot Module set is an electrophoresis and blotting system for mini gels. It includes the XCell SureLock Mini-Cell for running Invitrogen mini gels and the XCell II Blot Module for blotting up to two mini gels at a time.

See all available electrophoresis chamber systems ›

How the system works
The XCell SureLock Mini-Cell holds gels firmly with a simple gel tension wedge. When the lever on the gel tension wedge is pushed forward into the locked position, an even, horizontal force is generated. This seals the gel/buffer core assembly firmly into position in the lower buffer chamber. The positive locking action of the gel tension wedge ensures a trouble-free, leak-free gel run every time.

XCell II Blot Module
The XCell II Blot Module allows you to easily transfer proteins or nucleic acids from mini gels to membranes. It fits into the XCell SureLock and XCell II Mini-Cells in place of the gel/buffer core assembly. It requires less than 200 mL of transfer buffer for western, southern, and northern transfers. Tough platinized titanium and stainless steel electrodes create a uniform electrical field without clamps or hinged gel holders. Maximum blot size is 9 cm x 9 cm.

Upgrade your XCell II to an XCell SureLock Mini-Cell
Conversion of an XCell II into an XCell SureLock is easy with the SureLock Retrofit Kit (available separately). The kit consists of a SureLock mini-cell lid, gel tension wedge, and molded mini-gel buffer dam.

Owl Gel Staining Box (Thermo Scientific™)

iBlot™ 2 and iBind™ Western Starter Kit (Invitrogen™)

The iBlot 2 and iBind Western Starter Kit includes the iBlot 2 Gel Transfer Device, the iBind Western Device, and all of the consumables and reagents necessary to get started using both devices for fast, efficient transfer and western processing.

Contents of the iBlot 2 and iBind Western Starter Kit:
iBlot 2 Gel Transfer Device
iBlot 2 Transfer Stacks, nitrocellulose, regular size
iBind Western Device
iBind Cards
iBind Solution Kit
SuperSignal West Pico Plus Chemiluminescent Substrate

iBlot 2 Gel Transfer Device
The iBlot 2 Gel Transfer Device performs western blotting transfer simply, efficiently, and reliably, within seven minutes without the need for liquid buffers. The iBlot 2 system is compatible with both polyvinylidene difluoride (PVDF) and nitrocellulose membranes, and has comparable performance to traditional wet transfer methods in a fraction of the time. The iBlot 2 Gel Transfer Device is an integral part of the iBlot 2 Dry Blotting system, which consists of the transfer device and consumable transfer stacks that contain the required buffers and transfer membrane.

iBind Western Device
The iBind Western Device is an automated western-processing device that performs every step in the western protocol from blocking to washes to antibody incubations via sequential lateral flow (SLF). SLF allows the timely release and flow of solutions and antibodies to the membrane without need of an external power source. Each solution is released from the iBind device wells to an iBind Card via SLF; the solutions are then wicked towards the stack region of the card. The glass fiber matrix of the card allows for homogenous and consistent flow of the solutions to the membrane, increasing the antigen-antibody interaction. The iBind western detection procedure minimizes hands-on time and is highly reproducible and compatible with all western protocols. Set up time is fast: in less than 3 hours you are ready for the detection step.

Learn more about the iBlot 2 system ›
Learn more about the iBind western device ›

BenchPro™ 4100 Western Card (Invitrogen™)

The BenchPro™ 4100 Western Card is a disposable, single-use card that is inserted into the slot on top of the BenchPro™ 4100 Card Processing Station. Developed for immunodetection, the card holds the membrane to be processed in a Blot Holder. During operation, reagents are pumped from reagent bottles and vials located in the reagent tray, into the card and around the membrane. The order of reagents used and timing of protocol steps are based on the protocol selected.

Owl™ Transfer Membranes (Thermo Scientific™)

Perform Western blotting with hydrophobic Thermo Scientific™ Owl™ Transfer Membranes.

Power Blotter Station (Invitrogen™)

The Invitrogen Power Blotter Station is a power supply with touchscreen user interface for semi-dry transfer of proteins from gel to membrane when used with Power Blotter Cassette or Power Blotter Cassette XL. The Power Blotter Station features pre-programmed electrophoretic protein transfer methods for different types, numbers, and sizes of gels and molecular weight ranges of proteins.

Features of the Power Blotter Station:
Easy-touch programming—intuitive, color LCD touchscreen and pre-programmed methods for rapid semi-dry transfer of proteins; software includes step-by-step instructions for quickly creating, saving and running custom programs
Flexible—efficiently transfer proteins in about 5–12 minutes with one system
Integrated power supply—seamless operation between control unit and cassettes enables consistent high efficiency protein staining and transfer
Convenient—simultaneous transfer of up to two mini-size or one midi-size gel using the Power Blotter Cassette, or transfer four mini-size or two midi-size gels using the Power Blotter Cassette XL

Learn more about the semi-dry transfer device: Power Blotter System

Power Blotter XL System (Invitrogen™)

The Invitrogen Power Blotter XL System is specifically designed for rapid semi-dry transfer of proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes in 5 to 12 minutes. The Power Blotter System includes an integrated power supply optimized to enable consistent and high-efficiency protein transfer.

Features of the Power Blotter XL System include:
Efficient—high transfer efficiency with a broad range of protein sizes compared to conventional semi-dry or wet (tank) transfer methods
Fast—5- to 12-minute transfer when used with Power Blotter Select Transfer Stacks or Power Blotter 1-Step Transfer Buffer
Integrated power supply—seamless operation between control unit and cassette; no need for external power supply
Easy-touch programming—convenient pre-programmed transfer methods or create, save, and run customized transfer methods
Flexible—simultaneous transfer of 1 to 4 mini-size or 1 to 2 midi-size gels with the Power Blotter Cassette XL
Versatile—compatible with Power Blotter Select Transfer Stacks and Pierce 1-Step Transfer Buffer for rapid blotting programs or Towbin transfer buffer for conventional semi-dry transfer methods

Learn more about the semi-dry transfer device: Power Blotter XL System

The Power Blotter XL Cassette, included in the Power Blotter XL System, enables the simultaneous transfer of up to four mini-size or two midi-size gels. The included Power Blotter Station has an easy-to-use color LCD touchscreen interface with pre-programmed transfer methods for different numbers and sizes of gels and ranges of molecular weight proteins. The easy-touch programming feature allows custom transfer settings to be quickly created, saved, and run.

The Power Blotter System achieves highly efficient transfer in seven minutes when used with Power Blotter Select Transfer Stacks or ten minutes with Power Blotter 1-Step Transfer Buffer. The instrument is also effective for standard 30- to 60-minute semi-dry transfer protocols based on traditional Towbin buffer.

Contents of the Power Blotter XL System:
Power Blotter Station
Power Blotter Cassette XL

Related products
Power Blotter System

iBlot™ 2 Gel Transfer Device

The iBlot 2 Gel Transfer Device is a dry transfer device that performs western blotting transfer efficiently and reliably, within seven minutes, and without the need for liquid buffers. The iBlot 2 system is compatible with both polyvinylidene difluoride (PVDF) and nitrocellulose membranes and has comparable performance to traditional wet-transfer methods in a fraction of the time. The iBlot 2 Gel Transfer Device is an integral part of the iBlot 2 Dry Blotting System, which consists of the transfer device and consumable transfer stacks that contain the required buffers and transfer membrane (nitrocellulose or PVDF).

Learn more about the iBlot 2 system ›

iBlot 2 Gel Transfer Devivce features include:
• Complete protein transfer in seven minutes or less
• High detection sensitivity and even transfer
• Increased blotting reliability and reproducibility
• A simple, user-friendly system
• Pre-programed with six voltage methods that allows blotting using different combinations of volts and time
• Ability to create custom programs
• Built-in tutorials and application notes
• Transfer proteins from mini-, midi-, and E-PAGE-sized gels

How the iBlot 2 Dry Blotting System works
Buffer ion reservoirs are incorporated into the gel matrix of transfer stacks instead of buffer tanks or soaked papers. The high density of ions in the gel matrix enables rapid protein transfer. During blotting, the copper anode does not generate oxygen gas as a result of water electrolysis, reducing blot distortion (conventional protein transfer techniques, including wet, semi-wet, and semi-dry, use inert electrodes that generate oxygen). With the iBlot 2 Dry Blotting System, transfer time is reduced by the shortened distance between electrodes, high field strength, and high current. Trapped air bubbles, often created during the manual preparation of the blotting sandwich layers, are easy to avoid due to our unique de-bubbling design that promotes even and complete transfer. With the iBlot 2 system, there is no need to prepare buffers, pre-treat your gel, or clean up after blotting. The total preparation and run time is normally less than ten minutes per blot.

Power Blotter Cassette (Invitrogen™)

The Invitrogen Power Blotter Cassette is part of the Power Blotter System designed specifically for rapid semi-dry transfer of proteins from polyacrylamide gels to nitrocellulose or PVDF membranes in 5 to 12 minutes. The Power Blotter Cassette can simultaneously transfer up to two mini-size or one midi-size gel. This item is made available as a replacement or additional cassette for the Power Blotter Station.

The Power Blotter Cassette can be used with Power Blotter Select Transfer Stacks for rapid transfers, the Power Blotter 1-Step Transfer Buffer for semi-dry transfers, or alternatively with traditional buffers for longer semi-dry transfers.

Learn more about the Power Blotter System ›

Related products
Power Blotter Cassette XL

Bolt™ Western Pack B (PVDF) (Invitrogen™)

The Bolt® Western Pack (PVDF) includes two Mini Blot Modules and all of the buffers and pre-cut membrane/filter paper sandwiches you will need for the wet transfer of proteins from Bolt® Bis-Tris Plus gels to PVDF membranes. Each pack contains:
Mini Blot Modules
Bolt® Transfer Buffer (20X)
Bolt® Antioxidant
PVDF pre-cut membranes (0.2 µm)

About the Mini Blot Module
The Mini Blot Module is a wet transfer device used exclusively with the Mini Gel Tank. The tank accommodates one blot module per chamber, or two blot modules total with the side-by-side layout. The Mini Blot Module is compatible with Bolt® Bis-Tris Plus gels and accommodates one gel per wet transfer. The universal connection and molded gasket make the blot module easy to use, while the inner core of the blot module allows for use of less methanol-based transfer buffer per wet transfer. At the recommended conditions and constant voltage, proteins can be transferred to nitrocellulose or PVDF membranes typically in 30 to 60 minutes.

iBind™ Western Device

The iBind Western Device is an automated western-processing device that performs every step from blocking to washes to antibody incubations via sequential lateral flow (SLF). SLF allows the timely release and flow of solutions and antibodies to the membrane without need of an external power source, shakers, or trays.

Learn more about the iBind western systems ›

The iBind Western Device works exclusively with iBind Cards and the iBind Solution Kit or iBind Fluorescent Detection (FD) Solution Kit to process one mini-membrane for immunodetection. The iBind device provides automated convenience and superior reproducibility over traditional manual methods. Each solution is released from the iBind device wells to an iBind Card via SLF in ~2.5 hours. The glass fiber matrix of the card allows for homogenous and consistent flow of the solutions over the membrane, increasing the antigen-antibody interaction.

Power Blotter XL Welcome Pack (Invitrogen™)

The Power Blotter XL Welcome Pack includes:
Power Blotter Station
Power Blotter Cassette XL
Power Blotter Select Transfer Stacks, nitrocellulose, regular size
Power Blotter Pre-cut Membranes and Filters, nitrocellulose, regular size
Power Blotter 1-Step Transfer Buffer

The Invitrogen Power Blotter XL System (Power Blotter Station + Power Blotter Cassette XL) is specifically designed for rapid semi-dry transfer of proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes in 5 to 12 minutes. The Power Blotter XL System includes an integrated power supply optimized to enable consistent and high-efficiency protein transfer.

Features of the Power Blotter XL System include:
Efficient—high transfer efficiency with a broad range of protein sizes compared to conventional semi-dry or wet (tank) transfer methods
Fast—5- to 12-minute transfer when used with Power Blotter Select Transfer Stacks or Power Blotter 1-Step Transfer Buffer
Integrated power supply—seamless operation between control unit and cassette; no need for external power supply
Easy-touch programming—convenient pre-programmed transfer methods or create, save, and run customized transfer methods
Flexible—simultaneous transfer of 1 to 4 mini-size or 1 to 2 midi-size gels using the Power Blotter XL Cassette
Versatile—compatible with Power Blotter Select Transfer Stacks and Pierce 1-Step Transfer Buffer for rapid blotting programs or Towbin transfer buffer for conventional semi-dry transfer methods

Learn more about the semi-dry transfer device: Power Blotter XL System

The Power Blotter System achieves highly efficient transfer in seven minutes when used with Power Blotter Select Transfer Stacks or ten minutes with Power Blotter 1-Step Transfer Buffer. The instrument is also effective for standard 30- to 60-minute semi-dry transfer protocols based on traditional Towbin buffer.

Related products
Power Blotter Welcome Pack