Shop All Cloning Competent Cells

One Shot™ TOP10/P3 Chemically Competent E. coli Invitrogen™

TOP10/P3 E. coliallow high-quality plasmid preparation of pCDM8, pcDNA™1.1, or any other supF-containing vector. The strain features:

• P3 plasmid for selection of supF-containing plasmids
• Mutated endA1 gene for increased quality of plasmid DNA preparations

MAX Efficiency™ DH5αF´IQ Competent Cells Invitrogen™

MAX Efficiency DH5αF´IQ Chemically Competent Cells are high-efficiency chemically competent cells available for M13 cloning, cloning into plasmids with an f1-like origin of replication (phagemids), or cloning into any vector that uses expression from the lac promoter.
MAX Efficiency DH5αF´IQ cells offer the following features:

• >3 x 108 transformants/µg efficiency
• Stable F´ episome eliminates need for growth on minimal media
• ZΔM15 for blue/white screening of colonies or plaques

Three milliliters of lawn cells and a vial of monomer M13mp18 RF DNA are supplied as a control.

One Shot™ Mach1™ T1 Phage-Resistant Chemically Competent E. coli Invitrogen™

One Shot® Mach1™ T1 Phage-Resistant Chemically Competent E. coli are the fastest growing chemically competent cells currently available (see figure). Doubling time is approximately 50 minutes, compared to >74 minutes for other cloning strains. Mach1™ colonies are clearly visible within eight hours of plating the transformation mix, enabling you to plate and pick colonies the same day. Perform mini-preps after only 4 hours of growth from an overnight colony—saving an entire day in your experiments. The fast-growing Mach1™ T1R strain:

• Minimizes nonspecific recombination in cloned DNA (recA)
• Offers resistance to T1 and T5 phage (tonA)
• Yields cleaner preparations of DNA and better results in downstream applications due to the elimination of nonspecific digestion by Endonuclease I (endA1)
• Provides efficient transformation of unmethylated DNA from PCR amplifications (hsdR)
• Allows blue/white screening of recombinant clones (lacZΔM15)

Two formats to choose from
• The convenient One Shot® format provides single-use, 50-µL aliquots that eliminate efficiency-zapping, freeze-thaw cycles and money wasted on unused cells
• The MultiShot™ StripWell format allows rapid, high-throughput transformation in a 96-well stripwell plate, which allows you to perform as few or as many transformations as you need without wasting reagents

ElectroMAX™ Stbl4™ Competent Cells Invitrogen™

ElectroMAX™ Stbl4™ Competent Cells are specifically designed for cloning unstable inserts. As electrocompetent cells, they have one of the highest transformation efficiencies available (>5×109 cfu/µg), making them ideal for generating cDNA and genomic libraries and for cloning unstable inserts. These electrocompetent E. coli:

• Are ideal for cloning unstable DNA—stabilizes direct repeat and retroviral sequences
• Permit efficient cloning of methylated genomic DNA
• Support blue/white screening
• Are designed to deliver high-yield plasmid preparations for downstream applications
• Provide transformation efficiencies of >5×109 cfu/µg

Propagating unstable and large DNA with high transformation-efficiency cells
Many competent cell strains have the recA1 genotype, which reduces recombination. However there are some instances when the DNA that you are trying to clone is still unstable in such cells, perhaps due to the presence of inverted or direct repeats, or GC-rich tracts. While such sequences are relatively common in eukaryotic genomes, they are rare in E. coli. Consequently, rearrangements may occur when these sequences are introduced into standard E. coli strains.

Using ElectroMAX™ Stbl4™ Cells
ElectroMAX™ Stbl4™ electrocompetent E. coli cells are a derivative of Stbl2™ cells and are ideal for the cloning of unstable inserts such as retroviral sequences, direct repeats, and tandem array genes. Furthermore, ElectroMAX™ Stbl4™ cells are useful for the generation of cDNA libraries using plasmid-derived vectors and are able to take up and maintain large plasmids (e.g., 50 kb cosmids and 100–200 kb P1 clones). The Stbl4™ cells also contain an F' episome, allowing them to serve as a host for single-stranded DNA such as M13mp cloning vectors. The lacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors, and can therefore be used for blue/white screening of colonies on agar plates containing Xgal or Bluo-gal and IPTG. The mcrA mutation and the mcrBC-hsdRMS-mrr deletion allow cloning of genomic sequences which are methylated. Finally, the endA1 mutation greatly increases plasmid yield and quality.

Genotype: mcrA Δ(mcrBC-hsdRMS-mrr) recA1 endA1 gyrA96 gal-thi-1 supE44 λ-relA1 Δ(lac-proAB)/F' proAB+lacIqZΔM15 Tn10 (TetR)

MultiShot™ FlexPlate Mach1™ T1R Competent Cells Invitrogen™

MultiShot FlexPlate Mach1 T1R chemically competent E. coli cells are cloning competent cells that are pre-aliquoted in a 96-well PCR plate to increase transformation productivity. The FlexPlate format can be divided down to any combination of 8-well segments for flexibility in transformation throughput. MultiShot FlexPlate Mach1 T1R competent E. coli cells are exceptionally fast-growing chemically competent cells, accelerating the time to clone evaluation.

All of our T1R strains have the tonA (fhuA) deletion in their genotype that confers resistance to T1 and T5 phage. T1 bacteriophage spread rapidly and lyse E. coli hosts, which are commonly used for cloning and library construction. A resistant genotype offers added security to protect valuable clones and libraries. This is especially important for genome and sequencing centers where T1 phage infection would be catastrophic.

Speedy Mach1 T1R cloning capabilities
MultiShot FlexPlate Mach1 T1R competent E. coli cells are fast growing. Mach1 colonies are clearly visible within eight hours of plating the transformation mix, enabling you to plate and pick colonies the same day. Perform mini-preps after only four hours of growth from an overnight colony—saving an entire day in your experiments. The Mach1 T1R strain is useful for many cloning applications and provides transformation efficiencies of >1 x 108 cfu/µg with control plasmid DNA.

Key features of the MultiShot FlexPlate Mach1 T1R Competent Cells include:
endA1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by endonuclease I
recA1398 for reduced occurrence of non-specific recombination in cloned DNA
hsdR for efficient transformation of unmethylated DNA from PCR amplifications
tonA (fhuA) for T1 and T5 phage resistance
lacZΔM15 for blue/white color screening of recombinant clones

MultiShot FlexWell format—increase transformation productivity
The FlexPlate is a 96-well PCR plate that can be used in automated liquid handling systems or divided into any combination of 8-well segments for flexibility in transformation throughput. Addition of DNA to the MultiShot FlexPlate Competent Cells can be done quickly with a multichannel pipet or a liquid handling system. After addition of DNA, MultiShot FlexPlate Competent Cells can efficiently be transformed by heat-shock using a water bath, heat block, or thermal cycler.

Key features of the MultiShot FlexPlate format include:
Increase throughput—compatible with automated liquid handling platforms
Flexible—transform in increments of 8 wells to entire 96-well plate
Convenient—competent cells pre-aliquoted into 96-well plate, ready for transformation

Genotype: F- φ80(lacZ)ΔM15 ΔlacX74 hsdR(rK-mK+) ΔrecA1398 endA1 tonA

Find the strain and format that you need
We offer many other E. coli strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs. If you require other high-throughput transformation options, choose from our collection of MultiShot formatted comp cells or email us for custom formatting options.

DH5α Competent Cells for Subcloning Thermo Scientific™

Thermo Scientific DH5α Competent Cells for Subcloning are chemically competent E. coli cells recommended for routine subcloning into plasmid vectors. Subcloning efficiency cells are not suitable for the generation of cDNA libraries. The φ80dlacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors to allow blue/white colony screening on bacterial agar plates containing Bluo-Gal or X-Gal.

• Transformation efficiency: >1x106 cfu/µg pUC19 DNA
• Suitable for routine cloning applications
• Convenient economical packaging
• Contain genetic markers that allow for blue/white colony screening

Applications
DH5α Competent Cells for Subcloning are suitable for the standard DNA cloning applications:
• DNA cloning following PCR or restriction digestion reactions
• Subcloning (transferring DNA from a parent vector to a destination vector)
• Plasmid isolation

Genotype
F– φ80lacZΔ M15 Δ (lacZYA-argF) U169 recA1 endA1 hsdR17 (rK– mK+) phoA supE44 λ- thi–1 gyrA96 relA1

ElectroMAX™ DH10B T1 Phage-Resistant Competent Cells Invitrogen™

ElectroMAX DH10B T1 Phage-Resistant Competent Cells offer transformation efficiencies that are among the highest achieved by any product we offer, and can exceed 1 x 1010 cfu/µg plasmid DNA in efficiency. They are ideal for applications requiring high-efficiency transformation, including cDNA and gDNA library construction. ElectroMAX DH10B cells:

• Maximize yield of transformants from limited cloning products
• Permit efficient cloning of methylated DNA
• Are resistant to T1 and T5 bacteriophages
• Support blue/white screening by α-complementation on plates containing X-Gal or Bluo-Gal
• Deliver high-yield plasmid preparations for downstream applications

Phage-resistant representative libraries from a single transformation
The tonA mutation in DH10B cells confers resistance to bacteriophages T1 and T5, safeguarding valuable libraries against contamination. Additionally, mutations in the methylation-dependent restriction system (mcrA, mcrBC, and mrr) make ElectroMAX DH10B T1 Phage-Resistant Cells ideal for construction of genomic libraries of both prokaryotic and eukaryotic genomic DNA, and allow for efficient plasmid rescue from eukaryotic genomes. ElectroMAX DH10B T1 Phage-Resistant Cells are suitable for construction of gene banks, for the generation of cDNA libraries using plasmid-derived vectors, and for situations when DNA is limiting. This strain also has the Φ80lacZΔM15 genotype, providing for the option of blue/white screening on plates containing either X-Gal or Bluo-Gal. Finally, the endA1 mutation makes DH10B an excellent vehicle for amplifying plasmid DNA for subsequent extraction and purification.

Genotype: F-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139Δ(ara, leu)7697 galU galK λ-rpsL nupG tonA

Find the strain and format that you need
DH10B cells are available in both electrocompetent and chemically competent formats. Our ElectroMAX DH10B cells are also suitable for cDNA or gDNA library construction but are not resistant to T1 and T5 phage infection. Our MegaX DH10B T1R Electrocomp Cells have the highest transformation efficiency (>3 x 1010 cfu/µg plasmid DNA) and also offer the benefit of T1 and T5 phage resistance.

MegaX DH10B T1R Electrocomp™ Cells Invitrogen™

MegaX DH10B T1R Electrocomp Cells are the highest-efficiency electrocompetent cells available. Improved production processes now guarantee a three-fold greater number of colonies per transformation (>3 x 1010 cfu/µg of pUC DNA), making the MegaX DH10B T1R cells ideal for highly demanding cloning and library construction. They have the same genotype as the widely used DH10B T1R strain, including tonA, which prevents infection by T1 and T5 lytic bacteriophages, and safeguards your valuable clones and libraries.

Using MegaX DH10B T1R cells
MegaX DH10B T1R cells provide highly efficient transformation of methylated DNA, since they lack the E. coli K12 restriction systems (mcrA Δ(mrr-hsdRMS-mcrBC)). The Φ80lacZΔM15 marker provides α-complementation of the β-galactosidase gene, allowing blue/white screening on agar plates containing X-gal or Bluo-gal.

Genotype: F- mcrA Δ(mrr-hsdRMS-mcrBC), Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG tonA

MultiShot™ StripWell Mach1™ T1 Phage-Resistant Chemically Competent E. coli Invitrogen™

MultiShot StripWell Mach1 T1R chemically competent E. coli cells are cloning competent cells that are packaged in a rack containing 12 strips of 8 tubes to increase productivity for medium-throughput bacterial transformations. MultiShot StripWell Mach1 T1R competent E. coli cells are exceptionally fast-growing chemically competent cells, accelerating the time to clone evaluation.

All of our T1R strains have the tonA (fhuA) deletion in their genotype that confers resistance to T1 and T5 phage. T1 bacteriophage spread rapidly and lyse E. coli hosts, which are commonly used for cloning and library construction. A resistant genotype offers added security to protect valuable clones and libraries. This is especially important for genome and sequencing centers where T1 phage infection would be catastrophic.

Speedy Mach1 T1R cloning capabilities
MultiShot FlexPlate Mach1 T1R Competent Cells are fast growing. Mach1 colonies are clearly visible within eight hours of plating the transformation mix, enabling you to plate and pick colonies the same day. Perform mini-preps after only four hours of growth from an overnight colony—saving an entire day in your experiments. The Mach1 T1R strain is useful for many cloning applications and provides transformation efficiencies of >1 x 108 cfu/µg with control plasmid DNA.

Key features of the MultiShot FlexPlate Mach1 T1R Competent Cells include:
endA1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by endonuclease I
recA1398 for reduced occurrence of non-specific recombination in cloned DNA
hsdR for efficient transformation of unmethylated DNA from PCR amplifications
tonA (fhuA) for T1 and T5 phage resistance
lacZΔM15 for blue/white color screening of recombinant clones

MultiShot StripWell format—flexible for no waste
Each StripWell of eight connected tubes is designed with the same single-tube, single-use features as our One Shot format tubes. This format allows for all steps of the transformation protocol, up to plating, to take place in the same tube, thereby saving time and preventing contamination. After addition of DNA, MultiShot StripWell competent cells can be transformed by heat-shock at 42°C using a water bath, all in the same tube.

The StripWell tubes can be cut to permit transformations in a single tube, eliminating wasted aliquots of cells and avoiding freeze-thaws that can result in reduced transformation efficiency.

Key features of the MultiShot StripWell format include:
Increased productivity—for medium throughput experimentation
Maximal flexibility—transform as few as 1 tube and as many as 96 from the StripWell rack
Familiar convenience—no aliquotting, add DNA directly to cells, heat shock, and recover in the same tube
Peace of mind—single-use transformation minimizes contamination

Genotype: F- φ80(lacZ)ΔM15 ΔlacX74 hsdR(rK-mK+) ΔrecA1398 endA1 tonA

Find the strain and format that you need
We offer many other E. coli strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs. If you require other high-throughput transformation options, choose from our collection of MultiShot formatted comp cells or email us for custom formatting options.

MultiShot™ TOP10 Chemically Competent E. coli Invitrogen™

MultiShot™ TOP10 Chemically Competent E. coli provide transformation efficiencies of >1 x 108 cfu/µg control plasmid DNA, and are suited for high-throughput transformations. MultiShot™ TOP10 Chemically Competent cells are packaged in five 96-well microtiter plates (15 µL aliquots) to simplify high-throughput bacterial transformations. Benefits of MultiShot™ TOP10 cells:

• Designed for high-throughput applications—microtiter plate format designed for automated high-throughput cloning
• Compatible with genomic DNA—the mcrA mutation supports efficient transfection of methylated DNA
• Permits rapid screening—the LacZΔM15 allele permits rapid blue/white screening of transfectants on plates containing X-Gal or Bluo-Gal
• Efficient—the endA1 mutation helps reduce endonuclease activity, improving subsequent plasmid DNA yields
• Reliable—the recA1 mutation helps reduce unwanted recombinationEasy to use for high-throughput transformations
MultiShot™ TOP10 Chemically Competent cells are genetically similar to the reliable DH10B™ strain, and are available in five 96-well plates to facilitate high-throughput transformation. After addition of DNA, MultiShot™ TOP10 Chemically Competent cells can be transformed by heat-shock at 42°C using a heat block or thermocycler. Using our TA Cloning® and TOPO® Cloning protocols, including controls, yields of 100–400 colonies per agar plate can be expected.

Genotype: F-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara, leu)7697 galU galK rpsL (StrR) endA1 nupG

Find the strain and format that you need
We also offer many other different strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs. If you require other high-throughput transformation options, choose from our collection of MultiShot™ formatted comp cells or contact us for custom formatting options.

One Shot™ ccdB Survival™ 2 T1R Competent Cells Invitrogen™

One Shot® ccdB Survival™ 2 T1R Chemically Competent Cells are suitable for the propagation of plasmids containing the ccdB gene, and are designed for use with the Gateway® Vector Conversion System and for propagating Gateway® destination, donor, and supercoiled entry vectors. Transformation efficiency is >1 × 109 transformants/µg plasmid DNA. The ccdB Survival™ 2 T1R E. coli strain is derived from the TOP10 strain. The ccdB Survival™ 2 T1R E. coli strain offers the following features:

• Resistance to the ccdB gene product
• Resistance to T1 and T5 phage (the tonA/fhuA phenotype)
• Supports high-yield preparation of supercoiled plasmid DNA (the endA1 phenotype)
• Reduced nonspecific recombination of cloned DNA (the recA1 phenotype)

Genotype: F-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araΔ139 Δ(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG fhuA::IS2

Library Efficiency™ DH5α Competent Cells Invitrogen™

Library Efficiency DH5α Competent Cells are a versatile strain of chemically competent cells that demonstrate transformation efficiencies of >1 x 108 cfu/µg plasmid DNA. Library Efficiency DH5α Competent Cells are ideal for difficult cloning constructions or any application that uses limiting amounts of DNA. Features of these cells:

• Supports blue/white screening by α-complementation on plates containing X-Gal or Bluo-Gal
• Maintains plasmid DNA with minimum recombination events
• Delivers high-yield plasmid preparations for downstream applications
• M13mp cloning vectors may be propagated on a lawn of DH5α-FT, DH5αF', DH5αF'IQ, JM101, or JM107 for plaque formation

Ideal for difficult cloning projects
Library Efficiency DH5α Competent Cells are recommended for routine cloning of genes into large plasmids, and are suitable for difficult clone constructions, such as blunt-end ligations. Library Efficiency DH5α Competent Cells have a mid-range transformation efficiency (>1 x 108 cfu/µg control DNA per 100 µL reaction). The strain also has the Φ80lacZΔM15 genotype, allowing blue/white screening on plates containing either X-Gal or Bluo-Gal. Finally, the recA1 mutation helps reduce the rate of recombination while propagating plasmid DNA, and the endA1 mutation makes DH5α cells an excellent choice for amplifying plasmid DNA for subsequent extraction and purification.

Genotype: F- Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(rk-, mk+) phoA supE44 thi-1 gyrA96 relA1 λ-

ElectroMAX™ DH10B Cells Invitrogen™

ElectroMAX DH10B Cells are electrocompetent E. coli cells offering the highest transformation efficiencies of >1 x 1010 cfu/µg plasmid DNA. ElectroMAX DH10B Cells are ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. ElectroMAX DH10B Cells provide:

• High-efficiency transformation to maximize clones
• Enhanced genetic markers for cDNA or gDNA cloning capabilities

Highest transformation efficiencies
ElectroMAX DH10B Cells offer the highest transformation efficiencies available with >1 x 1010 transformants/µg of control DNA in a 20 µL reaction. These high transformation efficiencies make ElectroMAX DH10B Cells ideal for efficient cloning of both prokaryotic and eukaryotic genomic DNA and efficient plasmid rescue from eukaryotic genomes. ElectroMAX DH10B Cells are also suitable for construction of gene banks, for generation of cDNA libraries using plasmid-derived vectors, and for situations when there is limited amount of DNA.

Note: A high-voltage electroporation apparatus capable of generating field strengths of 16 kV/cm is required.

Versatile cloning capabilities
ElectroMAX DH10B Cells have the following features:

• Φ80lacZΔM15 marker provides α-complementation of the β-galactosidase gene allowing blue/white screening on agar plates containing X-gal or Bluo-gal
mcrA genotypic marker and the mcrBC, mrr deletion allows for cloning DNA that contains methylcytosine and methyladenine
endA1 mutation enables increased plasmid yield and quantity

Genotype: F-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139Δ(ara, leu)7697 galU galK λ-rpsL nupG

Find the strain and format that you need
DH10B cells are available in both electrocompetent and chemically competent formats. Our ElectroMax DH10B T1R Cells and MegaX DH10B T1R Electrocomp Cells offer the benefit of T1 and T5 phage resistance.

DH5α Competent Cells Thermo Scientific™

Thermo Scientific DH5α Competent Cells are high efficiency, chemically competent E. coli cells ideal for construction of gene banks or generation of cDNA libraries using plasmid-derived vectors. The φ80dlacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors to allow blue/white colony screening on bacterial agar plates containing Bluo-Gal or X-Gal. RecA1 and endA1 mutations in DH5α cells improve insert stability and quality of the extracted plasmid DNA.

• High transformation efficiency: >1x109 cfu/µg pUC19 DNA
• Suitable for routine and high-throughput cloning applications
• Genetic markers that allow for blue/white colony screening
• Convenient two reactions per vial packaging
• S.O.C. Outgrowth medium included

Applications
DH5α Competent Cells are suitable for variety of applications requiring high transformation efficiency:
• Efficient DNA cloning derived from PCR, cDNA-generation reactions
• recA1 marker facilitates working with difficult to transform DNA
• Ideal for generation of cDNA libraries using plasmid-derived vectors
• Site-directed mutagenesis

Genotype
F– φ80lacZΔ M15 Δ (lacZYA-argF) U169 recA1 endA1 hsdR17 (rK– mK+) phoA supE44 λ- thi–1 gyrA96 relA1

MAX Efficiency™ DH10B Competent Cells Invitrogen™

MAX Efficiency DH10B Competent Cells are highly efficient E. coli, perfect for most applications. DH10B E. coli are available in both electrocompetent and chemically competent formats. The high transformation efficiency makes them ideal for generating cDNA or genomic libraries. High-efficiency transformation is seen with plasmids 150 kb in size. The versatile DH10B genotype has the following features:

lacZ for blue/white screening of recombinant clones
• Elimination of mcrA, mcrBC, mrr, and hsdRMS restriction systems to allow construction of more representative genomic libraries
• A1 mutation to increase plasmid yield and quantity
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