Shop All Cloning Competent Cells

One Shot™ INVαF´ Chemically Competent E. coli (Invitrogen™)

INVαF´ E. coliallow stable replication of high-copy number plasmids. The genotype of INVαF´ offers the following features:

• Blue/white screening of recombinant clones (lacZΔM15)
• Reduction of homologous recombination of transformed plasmids (recA)
• Increased quality of plasmid DNA preparations (endA1)
• Production and rescue of single-strand DNA from vectors carrying the f1 origin of replication (F´)

MC1061/P3 Chemically Competent E. coli (Invitrogen™)

MC1061/P3 Chemically Competent E. coli are used for highly efficient transformation of vectors that require the P3 episome for selection and maintenance (i.e., pCDM8, pcDNA™1.1, or any other supF-containing vector).

Contents
• 5 x 300 μL competent MC1061/P3 E. coli
• 50 µL pUC19 plasmid DNA (10 pg/μL in 5 mM Tris-HCl, 0.5 mM EDTA, pH 8)
• 15 mL SOC medium

MaV203 Competent Yeast Cells, Subcloning Scale (Invitrogen™)

MaV203 Competent Yeast Cells are designed for use with the ProQuest™ Two-Hybrid System (Figure 1). S. cerevisiae strain MaV203 contains deletions in the endogenous GAL4 and GAL80 genes for use with GAL4-based two-hybrid systems. The strain also has leu2 and trp1 mutations for selection of ProQuest™ bait and prey vectors. MaV203 contains three GAL4 inducible reporter genes, providing four phenotypes, for easier identification of true interactors (Figure 2). The MaV203 competent cells feature:

• Transformation (250 µl of cells) efficiency yields >1 x 106 colonies
• Control DNA pMAB12 to confirm transformation efficiency and the preparation of selection media
• Pre-qualified PEG/Lithium Acetate to maximize number of trans-formants
• Competent cells formatted with carrier DNA already included

TOP10 Electrocomp™ Kit (Invitrogen™)

TOP10 E. coli are provided at a transformation efficiency of 1 × 1010 cfu/µg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation. They allow stable replication of high-copy number plasmids.

The genotype of TOP10 cells is similar to the DH10B™strain, and offers the following features:

hsdR for efficient transformation of unmethylated DNA from PCR amplifications
mcrA for efficient transformation of methylated DNA from genomic preparations
lacZΔM15 for blue/white color screening of recombinant clones
endA1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by Endonuclease I
recA1 for reduced occurrence of non-specific recombination in cloned DNA

One Shot® TOP10 Kits are available with either chemically competent or electrocompetent E. coli to fit your specific transformation needs. TOP10 E. coli are also available in the high-throughput MultiShot™ format.

Note: This kit contains enough electrocompetent cells for 25 x 40 µL rxns or 50 x 20 µL rxns. Please see the product manual for reaction scale recommendations.

Genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupG

MultiShot™ FlexPlate OmniMAX™ 2 T1R Competent Cells (Invitrogen™)

MultiShot FlexPlate OmniMAX 2 T1R chemically competent E. coli cells are a superior strain of cloning competent cells that are pre-aliquoted in a 96-well PCR plate to increase transformation productivity. The FlexPlate format can be divided down to any combination of 8-well segments for flexibility in transformation throughput.

All of our T1R strains have the tonA (fhuA) deletion in their genotype that confers resistance to T1 and T5 phage. T1 bacteriophage spread rapidly and lyse E. coli hosts, which are commonly used for cloning and library construction. A resistant genotype offers added security to protect valuable clones and libraries. This is especially important for genome and sequencing centers where T1 phage infection would be catastrophic.

Abundance of colonies with OmniMAX 2 T1R cloning capabilities
MultiShot FlexPlate OmniMAX 2 T1R cells are an improved chemically competent E. coli cell line, perfect for use in all cloning applications, including Gateway cloning, and provide transformation efficiencies in the MultiShot FlexPlate format of >5 x 108 cfu/µg with control plasmid DNA. These cells also provide efficient transformation of highly methylated DNA, since OmniMAX 2 T1R cells lack the E. coli K-12 restriction systems (mcrA Δ(mrr hsdRMS-mcrBC)).

Key features of the MultiShot FlexPlate OmniMAX 2 T1R Competent Cells include:
Δ(ccdAB) for sensitivity to the toxic effects of the ccdB gene product, allowing negative selection of vectors containing the ccdB gene—ideal for transformation of Gateway and TOPO reactions
mcrA, mcrBC, mrr, and hsdRMS elimination of restriction systems to allow construction of more representative genomic constructs
tonA (fhuA) genotype to confer resistance to T1 and T5 phage
Highest transformation efficiency in FlexWell format (>5x108 cfu/µg control DNA)
endA1 for increased plasmid yield and quantity
recA1 for reduced occurrence of non-specific recombination in cloned DNA
lacZΔM15 for blue/white color screening of recombinant clones

MultiShot FlexWell format—increase transformation productivity
The FlexPlate is a 96-well PCR plate that can be used in automated liquid handling systems or divided into any combination of 8-well segments for flexibility in transformation throughput. Addition of DNA to the MultiShot FlexPlate Competent Cells can be done quickly with a multichannel pipet or a liquid handling system. After addition of DNA, the cells can be transformed efficiently by heat-shock using a water bath, heat block, or thermal cycler.

Key features of the MultiShot FlexPlate format include:
Increase throughput—compatible with automated liquid handling platforms
Flexible—transform in increments of 8 wells to entire 96-well plate
Convenient—competent cells pre-aliquoted into 96-well plate, ready for transformation

Genotype: F´ {proAB lacIq lacZΔM15 Tn10(TetR ) Δ(ccdAB)} mcrA Δ(mrr hsdRMS-mcrBC) Φ 80(lacZ)ΔM15 Δ(lacZYA-argF)U169 endA1 recA1 supE44 thi-1 gyrA96 relA1 tonA panD

Find the strain and format that you need
We offer many other E. coli strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs. If you require other high-throughput transformation options, choose from our collection of MultiShot formatted comp cells or email us for custom formatting options.

One Shot™ TOP10F´ Chemically Competent E. coli (Invitrogen™)

One Shot® TOP10F´ Chemically Competent E. coli are identical to TOP10 cells, with the addition of an F´ episome. TOP10 competent cells are provided at a transformation efficiency of 1 x 109 cfu/µg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation.

Using One Shot® TOP10F´ Chemically Competent E. coli
The F´ episome carries the tetracycline resistance gene and allows isolation of single-stranded DNA from vectors that have an f1 origin of replication. In addition, the F´ episome carries the lacIq repressor for inducible expression from trc, tac, and lac promoters using IPTG. TOP10F´ cells require IPTG induction for blue/white color screening.

MAX Efficiency™ DH5αF´IQ Competent Cells (Invitrogen™)

MAX Efficiency DH5αF´IQ Chemically Competent Cells are high-efficiency chemically competent cells available for M13 cloning, cloning into plasmids with an f1-like origin of replication (phagemids), or cloning into any vector that uses expression from the lac promoter.
MAX Efficiency DH5αF´IQ cells offer the following features:

• >3 x 108 transformants/µg efficiency
• Stable F´ episome eliminates need for growth on minimal media
• ZΔM15 for blue/white screening of colonies or plaques

Three milliliters of lawn cells and a vial of monomer M13mp18 RF DNA are supplied as a control.

One Shot™ MAX Efficiency™ DH10B T1 Phage-Resistant Cells (Invitrogen™)

DH10B T1 Phage-Resistant E. colihave all the benefits of the DH10B genotype, with the addition of the tonA genotype which confers resistance to T1 and T5 phage infection. The inclusion of the tonA genotype protects your valuable samples and minimizes the possibility of downtime in your lab due to phage contamination. The DH10B-T1R genotype has the following features:

tonA genotype to confer resistance to T1 and T5 phage
• Elimination of mcrA, mcrBC, mrr, and hsdRMS restriction systems to allow construction of more representative genomic libraries (1,2)
• The endA1 mutation to increase plasmid yield and quantity
ZΔM15 for blue/white screening of recombinant clones

DH10B-T1R E. coliare available in both electrocompetent and chemically competent formats. The high transformation efficiency makes them ideal for generating cDNA or genomic libraries. High efficiency transformation is seen with plasmids 150 kb in size (3).

TOP10F´ Electrocomp™ Kit (Invitrogen™)

The TOP10F' genotype is identical to TOP10 with the addition of an F' episome. The F' episome carries the tetracycline resistance gene and allows isolation of single-stranded DNA from vectors that have an f1 origin of replication. In addition, the F' carries the lacIq repressor for inducible expression from trc, tac, and lac promoters using IPTG. TOP10F' require IPTG induction for blue/white color screening.

The TOP10F' genotype offers the following features:

hsdR for efficient transformation of unmethylated DNA from PCR amplifications
mcrA for efficient transformation of methylated DNA from genomic preparations
lacZΔM15 for blue/white color screening of recombinant clones
endA1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by Endonuclease I
recA1 for reduced occurrence of non-specific recombination in cloned DNA
lacIq repressor for inducible expression from trc, tac, and lac promoters

Note: This kit contains enough electrocompetent cells for 25 x 40 µL rxns or 50 x 20 µL rxns. Please see the product manual for reaction scale recommendations.

Genotype:
F'{lacIqTn10(TetR)} mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupG

One Shot™ TOP10 Electrocomp™ E. coli (Invitrogen™)

One Shot® TOP10 Electrocomp™ E. coli are provided at a transformation efficiency of 1 x 1010 cfu/ µg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation. They allow stable replication of high copy number plasmids. The genotype of TOP10 cells is similar to that of the DH10B™ strain, and offers the following features:

hsdR for efficient transformation of unmethylated DNA from PCR amplifications
mcrA for efficient transformation of methylated DNA from genomic preparations
lacZΔM15 for blue/white color screening of recombinant clones
endA1 for cleaner preparations of DNA—get better results in downstream applications due to the elimination of nonspecific digestion by Endonuclease I
recA1 for reduced occurrence of nonspecific recombination in cloned DNA

Kit options
One Shot® TOP10 Kits are available with either chemically competent or electrocompetent E. coli to fit your specific transformation needs. TOP10 E. coli are also available in the high-throughput MultiShot™ format.

One Shot™ Mach1™ T1 Phage-Resistant Chemically Competent E. coli (Invitrogen™)

One Shot® Mach1™ T1 Phage-Resistant Chemically Competent E. coli are the fastest growing chemically competent cells currently available (see figure). Doubling time is approximately 50 minutes, compared to >74 minutes for other cloning strains. Mach1™ colonies are clearly visible within eight hours of plating the transformation mix, enabling you to plate and pick colonies the same day. Perform mini-preps after only 4 hours of growth from an overnight colony—saving an entire day in your experiments. The fast-growing Mach1™ T1R strain:

• Minimizes nonspecific recombination in cloned DNA (recA)
• Offers resistance to T1 and T5 phage (tonA)
• Yields cleaner preparations of DNA and better results in downstream applications due to the elimination of nonspecific digestion by Endonuclease I (endA1)
• Provides efficient transformation of unmethylated DNA from PCR amplifications (hsdR)
• Allows blue/white screening of recombinant clones (lacZΔM15)

Two formats to choose from
• The convenient One Shot® format provides single-use, 50-µL aliquots that eliminate efficiency-zapping, freeze-thaw cycles and money wasted on unused cells
• The MultiShot™ StripWell format allows rapid, high-throughput transformation in a 96-well stripwell plate, which allows you to perform as few or as many transformations as you need without wasting reagents

ElectroMAX™ Stbl4™ Competent Cells (Invitrogen™)

ElectroMAX™ Stbl4™ Competent Cells are specifically designed for cloning unstable inserts. As electrocompetent cells, they have one of the highest transformation efficiencies available (>5×109 cfu/µg), making them ideal for generating cDNA and genomic libraries and for cloning unstable inserts. These electrocompetent E. coli:

• Are ideal for cloning unstable DNA—stabilizes direct repeat and retroviral sequences
• Permit efficient cloning of methylated genomic DNA
• Support blue/white screening
• Are designed to deliver high-yield plasmid preparations for downstream applications
• Provide transformation efficiencies of >5×109 cfu/µg

Propagating unstable and large DNA with high transformation-efficiency cells
Many competent cell strains have the recA1 genotype, which reduces recombination. However there are some instances when the DNA that you are trying to clone is still unstable in such cells, perhaps due to the presence of inverted or direct repeats, or GC-rich tracts. While such sequences are relatively common in eukaryotic genomes, they are rare in E. coli. Consequently, rearrangements may occur when these sequences are introduced into standard E. coli strains.

Using ElectroMAX™ Stbl4™ Cells
ElectroMAX™ Stbl4™ electrocompetent E. coli cells are a derivative of Stbl2™ cells and are ideal for the cloning of unstable inserts such as retroviral sequences, direct repeats, and tandem array genes. Furthermore, ElectroMAX™ Stbl4™ cells are useful for the generation of cDNA libraries using plasmid-derived vectors and are able to take up and maintain large plasmids (e.g., 50 kb cosmids and 100–200 kb P1 clones). The Stbl4™ cells also contain an F' episome, allowing them to serve as a host for single-stranded DNA such as M13mp cloning vectors. The lacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors, and can therefore be used for blue/white screening of colonies on agar plates containing Xgal or Bluo-gal and IPTG. The mcrA mutation and the mcrBC-hsdRMS-mrr deletion allow cloning of genomic sequences which are methylated. Finally, the endA1 mutation greatly increases plasmid yield and quality.

Genotype: mcrA Δ(mcrBC-hsdRMS-mrr) recA1 endA1 gyrA96 gal-thi-1 supE44 λ-relA1 Δ(lac-proAB)/F' proAB+lacIqZΔM15 Tn10 (TetR)

ElectroMAX™ DH10B Cells (Invitrogen™)

ElectroMAX DH10B Cells are electrocompetent E. coli cells offering the highest transformation efficiencies of >1 x 1010 cfu/µg plasmid DNA. ElectroMAX DH10B Cells are ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. ElectroMAX DH10B Cells provide:

• High-efficiency transformation to maximize clones
• Enhanced genetic markers for cDNA or gDNA cloning capabilities

Highest transformation efficiencies
ElectroMAX DH10B Cells offer the highest transformation efficiencies available with >1 x 1010 transformants/µg of control DNA in a 20 µL reaction. These high transformation efficiencies make ElectroMAX DH10B Cells ideal for efficient cloning of both prokaryotic and eukaryotic genomic DNA and efficient plasmid rescue from eukaryotic genomes. ElectroMAX DH10B Cells are also suitable for construction of gene banks, for generation of cDNA libraries using plasmid-derived vectors, and for situations when there is limited amount of DNA.

Note: A high-voltage electroporation apparatus capable of generating field strengths of 16 kV/cm is required.

Versatile cloning capabilities
ElectroMAX DH10B Cells have the following features:

• Φ80lacZΔM15 marker provides α-complementation of the β-galactosidase gene allowing blue/white screening on agar plates containing X-gal or Bluo-gal
mcrA genotypic marker and the mcrBC, mrr deletion allows for cloning DNA that contains methylcytosine and methyladenine
endA1 mutation enables increased plasmid yield and quantity

Genotype: F-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139Δ(ara, leu)7697 galU galK λ-rpsL nupG

Find the strain and format that you need
DH10B cells are available in both electrocompetent and chemically competent formats. Our ElectroMax DH10B T1R Cells and MegaX DH10B T1R Electrocomp Cells offer the benefit of T1 and T5 phage resistance.

Library Efficiency™ DH5α Competent Cells (Invitrogen™)

Library Efficiency DH5α Competent Cells are a versatile strain of chemically competent cells that demonstrate transformation efficiencies of >1 x 108 cfu/µg plasmid DNA. Library Efficiency DH5α Competent Cells are ideal for difficult cloning constructions or any application that uses limiting amounts of DNA. Features of these cells:

• Supports blue/white screening by α-complementation on plates containing X-Gal or Bluo-Gal
• Maintains plasmid DNA with minimum recombination events
• Delivers high-yield plasmid preparations for downstream applications
• M13mp cloning vectors may be propagated on a lawn of DH5α-FT, DH5αF', DH5αF'IQ, JM101, or JM107 for plaque formation

Ideal for difficult cloning projects
Library Efficiency DH5α Competent Cells are recommended for routine cloning of genes into large plasmids, and are suitable for difficult clone constructions, such as blunt-end ligations. Library Efficiency DH5α Competent Cells have a mid-range transformation efficiency (>1 x 108 cfu/µg control DNA per 100 µL reaction). The strain also has the Φ80lacZΔM15 genotype, allowing blue/white screening on plates containing either X-Gal or Bluo-Gal. Finally, the recA1 mutation helps reduce the rate of recombination while propagating plasmid DNA, and the endA1 mutation makes DH5α cells an excellent choice for amplifying plasmid DNA for subsequent extraction and purification.

Genotype: F- Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(rk-, mk+) phoA supE44 thi-1 gyrA96 relA1 λ-

MultiShot™ FlexPlate DH5α T1R Competent Cells (Invitrogen™)

MultiShot FlexPlate DH5α T1R chemically competent E. coli cells are cloning competent cells that are pre-aliquoted in a 96-well PCR plate to increase transformation productivity. The FlexPlate format can be divided down to any combination of 8-well segments for flexibility in transformation throughput.

MultiShot FlexPlate DH5α T1R competent E. coli cells are derived from the popular DH5α strain, one of the most frequently used laboratory cloning strains. All of our T1R strains have the tonA (fhuA) deletion in their genotype that confers resistance to T1 and T5 phage. T1 bacteriophage spread rapidly and lyse E. coli hosts, which are commonly used for cloning and library construction. A resistant genotype offers added security to protect valuable clones and libraries. This is especially important for genome and sequencing centers where T1 phage infection would be catastrophic.

Reliable DH5α T1R cloning capabilities
The DH5α strain is the standard laboratory strain for chemical transformations as it is ideal for transformations of cloned unmethylated DNA. The DH5α T1R strain is useful for many cloning applications and provides transformation efficiencies of >1 x 108 cfu/µg with control plasmid DNA.

Key features of the MultiShot FlexPlate DH5α T1R Competent Cells include:
hsdR for efficient transformation of unmethylated DNA from PCR amplifications
lacZΔM15 for blue/white color screening of recombinant clones
endA1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by endonuclease I
recA1 for reduced occurrence of non-specific recombination in cloned DNA
tonA (fhuA) for T1 and T5 phage resistance

MultiShot FlexWell format—increase transformation productivity
The FlexPlate is a 96-well PCR plate that can be used in automated liquid handling systems or divided into any combination of 8-well segments for flexibility in transformation throughput. Addition of DNA to MultiShot FlexPlate Competent Cells can be done quickly with a multichannel pipet or liquid handling system. After addition of DNA, the cells can be transformed efficiently by heat-shock using a water bath, heat block, or thermal cycler.

Key features of the MultiShot FlexPlate format include:
Increase throughput—compatible with automated liquid handling platforms
Flexible—transform in increments of 8 wells to entire 96-well plate
Convenient—competent cells pre-aliquoted into 96-well plate, ready for transformation

Genotype: F- φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK-, mK+) phoA supE44 thi-1 gyrA96 relA1 tonA

Find the strain and format that you need
We offer many other E. coli strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs. If you require other high-throughput transformation options, choose from our collection of MultiShot formatted comp cells or email us for custom formatting options.