Shop All PBS (Non-Cell Culture) & TBS

PBS (Phosphate-Buffered Saline) Tablets (Invitrogen™)

Phosphate-Buffered Saline (PBS) is a balanced salt solution used for a variety of applications, including reagent preparation, diluting cells for flow cytometry, and as a cell culture reagent. These PBS tablets are not intended for cell culture as they are not tested for sterility. We offer a variety of Gibco® PBS formulations for cell culture applications. Find the right cell culture product using the media selector tool.

BupH™ Tris Buffered Saline Packs (Thermo Scientific™)

Thermo Scientific BupH Tris Buffered Saline Packs are pouches of dry-blend powder that are each sufficient to make 500 mL of Tris-NaCl buffer (TBS) for diluents and wash buffers in ELISA, Western blotting and general immunoassay procedures.

Features of Thermo Scientific BupH Tris Buffered Saline Packs:

TBS—dissolved in 500 mL of water, each pack makes 25 mM Tris, 0.15M NaCl, pH 7.2
Convenient—dissolve contents of one envelope in water and the buffer is ready to use
Save time and trouble—no weighing, no pH adjustment, no need to stock individual components, and no need to make and store large volumes of stock solution in advance of daily needs
Long shelf life—stocking and storage as dry packs eliminates concerns about long-term stability of stock solutions
Eliminate variables—our quality control ensures that every pack will yield the same, consistent buffer

Applications:
• Wash buffer and antibody diluent for ELISA, Western blotting and other immunoassays

BupH Dry-Blend Packs of TBS are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water and stir to dissolve. The packs eliminate weighing time and tedious pH adjustments. When dissolved in 500 mL of water, each pack makes 25 mM Tris, 0.15M NaCl, pH 7.2.

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Pierce™ 20X TBS Buffer

Pierce™ 20X TBS Buffer (Thermo Scientific™)

Thermo Scientific Pierce 20X Tris Buffered Saline is a space-saving stock solution that is ideal for preparing Tris-NaCl buffer (TBS) for use as a wash buffer and antibody diluent for ELISA, Western blotting and other immunoassays.

Features of Thermo Scientific Pierce 20X Tris Buffered Saline:

TBS—diluted 20-fold in water, the solution yields 25 mM Tris, 0.15M NaCl, pH 7.2 to 7.5
Easy to use—no packets to open and no powder to dissolve
Increased accuracy—eliminates the possibility of powder remaining in a packet
Saves time—20X concentration eliminates time spent waiting for powder to dissolve
Saves space—storage as concentrated stock minimizes bench space needed for solutions

Applications:
• Wash buffer and antibody diluent for ELISA, Western blotting and other immunoassays

Pierce Concentrated Buffers are ready to use without having to weigh and dissolve dry ingredients or to adjust the pH with concentrated acid or base. Simply dilute the stock solution with pure water and proceed with your experiment. The 20X Tris Buffered Saline (TBS) makes 25 mM Tris, 0.15M NaCl, pH 7.2 to 7.5, when diluted to 1X with water.

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BupH™ Tris Buffered Saline Packs

BupH™ Borate Buffer Packs (Thermo Scientific™)

Thermo Scientific BupH Borate Buffer Packs are pouches of dry-blended powder that are each sufficient to make 500 mL of borate buffer for use in protein modification procedures requiring amine-free conditions and alkaline pH.

Features of BupH Borate Buffer Packs:

Borate buffer—dissolved in 500 mL of water, each pack makes 50 mM borate, pH 8.5
Convenient—dissolve contents of one envelope in water and the buffer is ready to use
Save time and trouble—no weighing, no pH adjustment, no need to stock individual components, and no need to make and store large volumes of stock solution in advance of daily needs
Long shelf life—stocking and storage as dry packs eliminates concerns about long-term stability of stock solutions
Eliminate variables—our quality control ensures that every pack will yield the same, consistent buffer

BupH Dry-Blend Packs of borate buffer are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water and stir to dissolve. The packs eliminate weighing time and tedious pH adjustments. When dissolved in 500 mL of water, each pack makes 50 mM borate, pH 8.5.

Applications:
• Protein modification procedures requiring amine-free buffer at alkaline pH

Related Products
Pierce™ 20X Borate Buffer

Pierce™ 20X TBS Tween™ 20 Buffer (Thermo Scientific™)

Thermo Scientific Pierce 20X TBS Tween-20 is a space-saving stock solution that is ideal for preparing TBS-Tween (TBS-T) wash buffers or blocking buffer diluent used for applications such as ELISA, Western blotting and other immunoassays.

Features of Thermo Scientific Pierce 20X TBS Tween-20:

TBS-T—diluted 20-fold in water, the solution yields 25 mM Tris, 0.15M NaCl, 0.05% Tween-20, pH 7.5
Easy to use—no packets to open and no powder to dissolve
Increased accuracy—eliminates the possibility of powder remaining in a packet
Saves time—20X concentration eliminates time spent waiting for powder to dissolve
Saves space—storage as concentrated stock minimizes bench space needed for solutions

Applications:
• Wash buffer or blocking buffer for ELISA, Western and other immunoassays

Pierce Concentrated Buffers are ready to use without having to weigh and dissolve dry ingredients or to adjust the pH with concentrated acid or base. Simply dilute the stock solution with pure water and proceed with your experiment. The 20X TBS Tween-20 (Tris-buffered saline with Tween™ 20 detergent) makes 25 mM Tris, 0.15M NaCl, 0.05% Tween-20, pH 7.5, when diluted to 1X with water.

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Pierce™ 20X TBS Buffer

BupH™ Phosphate Buffered Saline Packs (Thermo Scientific™)

Thermo Scientific BupH Phosphate Buffered Saline Packs are pouches of dry-blend powder that are each sufficient to make 500 mL of sodium phosphate buffer (PBS) for use in crosslinking and labeling reactions requiring amine-free conditions.

Features of BupH Phosphate Buffered Saline Packs:

PBS—dissolved in 500 mL of water, each pack makes 0.1M sodium phosphate, 0.15M NaCl, pH 7.2
Convenient—dissolve contents of one envelope in water and the buffer is ready to use
Save time and trouble—no weighing, no pH adjustment, no need to stock individual components, and no need to make and store large volumes of stock solution in advance of daily needs
Long shelf life—stocking and storage as dry packs eliminates concerns about long-term stability of stock solutions
Eliminate variables—our quality control ensures that every pack will yield the same, consistent buffer

BupH Dry-Blend Packs of PBS are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water and stir to dissolve. The packs eliminate weighing time and tedious pH adjustments. When dissolved in 500 mL of water, each pack makes 0.1M sodium phosphate, 0.15M NaCl, pH 7.2.

Applications:
• Crosslinking, biotinylation and fluorescent labeling reactions requiring an amine-free buffer

Related Products
Pierce™ 20X Phosphate Buffered Saline

BupH™ MES Buffered Saline Packs (Thermo Scientific™)

Thermo Scientific BupH MES Buffered Saline Packs are pouches of dry-blended powder that are each sufficient to make 500 mL of optimized amine-free and carboxyl-free buffer for carbodiimide (EDC) crosslinking and conjugation reactions.

Features of BupH MES Buffered Saline Packs:

MES buffer—dissolved in 500 mL of water, each pack makes 0.1M MES, 0.9% sodium chloride, pH 4.7
Convenient—dissolve contents of one envelope in water and the buffer is ready to use
Save time and trouble—no weighing, no pH adjustment, no need to stock individual components, and no need to make and store large volumes of stock solution in advance of daily needs
Long shelf life—stocking and storage as dry packs eliminates concerns about long-term stability of stock solutions
Eliminate variables—our quality control ensures that every pack will yield the same, consistent buffer

BupH Dry-Blend Packs of MES buffered saline are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water and stir to dissolve. The packs eliminate weighing time and tedious pH adjustments. When dissolved in 500 mL of water, each pack makes 0.1M MES, 0.9% sodium chloride, pH 4.7. MES is 2-(N-morpholino) ethanesulfonic acid.

Applications:
• Carboxyl-to-amine crosslinking procedures using carbodiimide crosslinker EDC (Part No. 22980)

PBS - Phosphate-Buffered Saline (10X) pH 7.4, RNase-free (Invitrogen™)

PBS (phosphate buffered saline) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions which, when diluted to a 1X working concentration, contains 137 mM NaCl, 2.7 mM KCl, 8 mM Na2HPO4, and 2 mM KH2PO4. Each 10X PBS solution is ready to use upon dilution to the desired concentration. This molecular biology-grade PBS is certified RNase-free; it is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.

BupH™ Modified Dulbecco's PBS Packs (Thermo Scientific™)

Thermo Scientific BupH Modified Dulbecco's PBS Packs are pouches of dry-blend powder that are each sufficient to make 500 mL of physiological Na- and K-phosphate buffered saline (D-PBS) for ELISA and Western blot diluents and wash buffers.

Features of BupH Modified Dulbecco's PBS Packs:

Dulbecco's PBS—dissolved in 500 mL of water, each pack makes 8 mM sodium phosphate, 2 mM potassium phosphate, 0.14M NaCl, 10 mM KCl, pH 7.4
Convenient—dissolve contents of one envelope in water and the buffer is ready to use
Save time and trouble—no weighing, no pH adjustment, no need to stock individual components, and no need to make and store large volumes of stock solution in advance of daily needs
Long shelf life—stocking and storage as dry packs eliminates concerns about long-term stability of stock solutions
Eliminate variables—our quality control ensures that every pack will yield the same, consistent buffer

BupH Dry-Blend Packs of modified Dulbecco's phosphate buffered saline are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water and stir to dissolve. The packs eliminate weighing time and tedious pH adjustments. When dissolved in 500 mL of water, each pack makes 8 mM sodium phosphate, 2 mM potassium phosphate, 0.14M NaCl, 10 mM KCl, pH 7.4.

Applications:
• Wash buffers and antibody diluents in applications such as ELISA, Western blotting and other immunoassays

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Pierce™ 20X Modified Dulbecco's PBS Buffer

Pierce™ 20X PBS Tween™ 20 Buffer (Thermo Scientific™)

Thermo Scientific Pierce 20X PBS Tween-20 is a space-saving stock solution that is ideal for preparing PBS-Tween (PBS-T) wash buffers for ELISA, Western and other immunoassays as well as a blocking buffer for plate-based assays.

Features of 20X PBS Tween-20:

PBS-T—diluted 20-fold in water, the solution yields 10 mM sodium phosphate, 0.15M NaCl, 0.05% Tween-20, pH 7.5
Easy to use—no packets to open and no powder to dissolve
Increased accuracy—eliminates the possibility of powder remaining in a packet
Saves time—20X concentration eliminates time spent waiting for powder to dissolve
Saves space—storage as concentrated stock minimizes bench space needed for solutions

Pierce Concentrated Buffers are ready to use without having to weigh and dissolve dry ingredients or to adjust the pH with concentrated acid or base. Simply dilute the stock solution with pure water and proceed with your experiment. The 20X PBS Tween-20 makes 10 mM sodium phosphate, 0.15M NaCl, 0.05% Tween™ 20, pH 7.5, when diluted to 1X with water.

Applications:
• A wash buffer for ELISA, Western blotting and other immunoassays
• Diluent or blocking buffer for plate-based assays

Related Products
Pierce™ 20X Phosphate Buffered Saline

BupH™ Carbonate-Bicarbonate Buffer Packs (Thermo Scientific™)

Thermo Scientific BupH Carbonate-Bicarbonate Buffer Packs are pouches of dry-blended powder that are each sufficient to make 500 mL of alkaline (pH 9.4) Na-carbonate (carb-bicarb) buffer for use in protein and antibody coating procedures.

BupH Dry-Blend Packs of carbonate-bicarbonate buffer are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water and stir to dissolve. The packs eliminate weighing time and tedious pH adjustments. When dissolved in 500 mL of water, each pack makes 0.2M carbonate-bicarbonate, pH 9.4.

Features of BupH Carbonate-Bicarbonate Buffer Packs:

Carb-bicarb buffer—dissolved in 500 mL of water, each pack makes 0.2M carbonate-bicarbonate, pH 9.4
Convenient—dissolve contents of one envelope in water and the buffer is ready to use
Save time and trouble—no weighing, no pH adjustment, no need to stock individual components, and no need to make and store large volumes of stock solution in advance of daily needs
Long shelf life—stocking and storage as dry packs eliminates concerns about long-term stability of stock solutions
Eliminate variables—our quality control ensures that every pack will yield the same, consistent buffer

Applications:
• Coating or binding capture-antibodies and other proteins onto polystyrene microplates for ELISA and other plate assays

Pierce™ 20X Phosphate Buffered Saline (Thermo Scientific™)

Thermo Scientific Pierce 20X Phosphate Buffered Saline is a space-saving stock solution that is ideal for preparing PBS buffers for use in crosslinking, biotinylation and fluorescent labeling reactions requiring an amine-free buffer.

Features of 20X Phosphate Buffered Saline:

PBS—diluted 20-fold in water, the solution yields 10 mM sodium phosphate, 0.15M NaCl, pH 7.5
Easy to use—no packets to open and no powder to dissolve
Increased accuracy—eliminates the possibility of powder remaining in a packet
Saves time—20X concentration eliminates time spent waiting for powder to dissolve
Saves space—storage as concentrated stock minimizes bench space needed for solutions

Pierce Concentrated Buffers are ready to use without having to weigh and dissolve dry ingredients or to adjust the pH with concentrated acid or base. Simply dilute the stock solution with pure water and proceed with your experiment. The 20X Phosphate Buffered Saline (PBS) makes 10 mM sodium phosphate, 0.15M NaCl, pH 7.5, when diluted to 1X with water.

Applications:
• Crosslinking, biotinylation and fluorescent labeling reactions requiring an amine-free buffer

Related Products
BupH™ Phosphate Buffered Saline Packs

TBS Buffer

Tris Buffered Saline (TBS) Buffer is intended for use with PanPath products. TBS is supplied as a lyophilized powder, and when dissolved in one liter distilled or deionised water, it becomes 0.05M TBS, pH 8.0 (0.238M NaCl; 0.0027M KCl) with 0.05% Tween 20.

Pierce™ 20X Modified Dulbecco's PBS Tween™ 20 Buffer (Thermo Scientific™)

Thermo Scientific Pierce 20X Modified Dulbecco's PBS Tween-20 is a space-saving stock solution used to prepare physiological Na- and K-phosphate buffered saline (D-PBS) wash and blocking buffers for ELISA, Western and other immunoassays.

Features of 20X Modified Dulbecco's PBS Tween-20:

Dulbecco's PBS-T—diluted 20-fold in water, the solution yields 8 mM sodium phosphate, 2 mM potassium phosphate, 0.14M NaCl, 10 mM KCl, 0.05% Tween-20, pH 7.4
Easy to use—no packets to open and no powder to dissolve
Increased accuracy—eliminates the possibility of powder remaining in a packet
Saves time—20X concentration eliminates time spent waiting for powder to dissolve
Saves space—storage as concentrated stock minimizes bench space needed for solutions

Pierce Concentrated Buffers are ready to use without having to weigh and dissolve dry ingredients or to adjust the pH with concentrated acid or base. Simply dilute the stock solution with pure water and proceed with your experiment. The 20X Modified Dulbecco's PBS Tween-20 (D-PBS-T) makes 8 mM sodium phosphate, 2 mM potassium phosphate, 0.14M NaCl, 10 mM KCl, 0.05% Tween™ 20, pH 7.4, when diluted to 1X with water.

Applications:
• A wash buffer for ELISA, Western blotting and other immunoassays
• Diluent or blocking buffer for plate-based assays

Related Products
Pierce™ 20X Modified Dulbecco's PBS Buffer

Pierce™ 20X Modified Dulbecco's PBS Buffer (Thermo Scientific™)

Thermo Scientific Pierce 20X Modified Dulbecco's PBS is a space-saving stock solution used for preparing physiological Na- and K-phosphate buffered saline (D-PBS) antibody diluents used in ELISA, Western blotting and other immunoassays.

Features of 20X Modified Dulbecco's PBS:

Dulbecco's PBS—diluted 20-fold in water, the solution yields 8 mM sodium phosphate, 2 mM potassium phosphate, 0.14M NaCl, 10 mM KCl, pH 7.4
Easy to use—no packets to open and no powder to dissolve
Increased accuracy—eliminates the possibility of powder remaining in a packet
Saves time—20X concentration eliminates time spent waiting for powder to dissolve
Saves space—storage as concentrated stock minimizes bench space needed for solutions

Pierce Concentrated Buffers are ready to use without having to weigh and dissolve dry ingredients or to adjust the pH with concentrated acid or base. Simply dilute the stock solution with pure water and proceed with your experiment. The 20X Modified Dulbecco's PBS (phosphate-buffered saline) makes 8 mM sodium phosphate, 2 mM potassium phosphate, 0.14M NaCl, 10 mM KCl, pH 7.4, when diluted to 1X with water.

Applications:
• Used for wash buffers and antibody diluents in applications such as ELISA, Western blotting and other immunoassays

Related Products
BupH™ Modified Dulbecco's PBS Packs