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Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Gibco™)

Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix is a soluble form of basement membrane extracted from murine Engelbreth-Holm-Swarm (EHS) tumors and is free of viruses, including lactose dehydrogenase elevating virus (LDEV), sometimes found in this type of preparation. The major components of Geltrex™ matrix include laminin, collagen IV, entactin, and heparin sulfate proteoglycans. It is ideal for the promotion and maintenance of differentiated phenotypes in a variety of cell cultures including primary epithelial cells, endothelial cells, smooth muscle cells, and human induced pluripotent stem cells (iPSC). Geltrex™ matrix offers, on average, a 2-fold reduction in several growth factors. As a result, analysis of specific growth factor effects in applications requiring basement membrane extract can be carried out in a controlled and reproducible format.

Key Features of Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix:

• Free of lactose dehydrogenase elevating virus (LDEV) making it ideal for all types of cell culture and mouse in vivo applications
• Consistent protein concentration lot-to-lot helps reduce the need to screen lots
• Protocols for various applications, including tube formation assays, are available

Gain Reliability and Consistency in Your hESC Studies
Recent advances in processing EHS tumor cells have resulted in virus free basement membrane extracts. Every lot is tested by RT-PCR methods so you can now have confidence that every lot of Geltrex™ is virus free. Additionally, our consistent manufacturing process helps ensure a protein concentration with very little variation from lot-to-lot, averaging 15 mg/ml so you can have confidence in every purchase.

Investigate Angiogenesis with Validated Gibco® Protocols
There are many applications for Geltrex™ LDEV-Free reduced growth factor basement membrane matrix, each requiring different thicknesses and concentrations. In general, a protein concentration of >5 mg/ml is used for differentiation studies of primary cells. Geltrex™ LDEV-Free reduced growth factor basement membrane matrix has been used to develop tube formation assay protocols using Gibco® primary cells and specialty media. See this and other angiogenesis assay protocols

Geltrex™ LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix (Gibco™)

Geltrex™ LDEV-Free hESC-qualified Reduced Growth Factor Basement Membrane Matrix is a soluble form of basement membrane extracted from murine Engelbreth-Holm-Swarm (EHS) tumors and is free of viruses, including lactose dehydrogenase elevating virus (LDEV), sometimes found in this type of preparation. Routinely used for attachment and maintenance of human embryonic stem cells (hESC), every lot of Geltrex™ LDEV-free hESC-qualified Reduced Growth Factor Basement Membrane Matrix is quality control tested using hESCs and StemPro hESC Serum Free Media to help ensure growth and pluripotency of each lot.

Key Features of Geltrex™ LDEV-Free hESC-qualified Reduced Growth Factor Basement Membrane Matrix:

• Every lot is qualified using hESC, which helps reduce the need for customers to lot screen
• Free of the lactose dehydrogenase elevating virus (LDEV) making it safe for all types of cell culture
• Consistent protein concentration lot-to-lot helps ensure reproducible results

Gain Reliability and Consistency in Your hESC Studies
Human ESCs have specific needs for attachment and maintenance and some lots of basement membrane extract may not provide adequate support. Geltrex™ hESC-qualified reduced growth factor basement membrane matrix has been tested for support of hESC growth and pluripotency, therefore minimizing the need for you to test multiple lots to find one that works. Our consistent manufacturing process helps ensure a protein concentration averaging 15 mg/ml with very little variation from lot-to-lot, so you can have confidence in every purchase.

Free of LDEV Contamination
Recent advances in processing EHS tumor cells have resulted in virus free basement membrane extracts. Every lot is tested by tested by RT-PCR methods so you can now have confidence that every lot of Geltrex™ is virus-free.

Geltrex™ hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix (Gibco™)

Geltrex hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix is a soluble form of basement membrane extracted from murine Engelbreth-Holm-Swarm (EHS) tumors. This pre-diluted version of the matrix can be taken directly from the refrigerator and dispensed into cell culture plates without further handling. Geltrex hESC-Qualified Ready-To-Use basement membrane matrix is optimized for stem cell culture applications using StemPro hESC SFM. For stem cell culture using the StemFlex Medium or Essential 8 Media System, use Geltrex LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix.

Geltrex hESC-Qualified Ready-To-Use matrix is:

• Consistent—pre-dilution at a concentration of 1:100 ensures optimal protein concentration for stem cell attachment to standard cell culture plates
• Stable—storage at 4°C eliminates overnight thawing on ice
• Cost effective— the ready-to-use format saves multiple preparation steps and potential for product failure

Reduced variability
Geltrex hESC-Qualified Ready-To-Use matrix is provided as a 1:100 dilution in a buffered solution that can be added directly to cell culture plates for coating. Each lot is prepared at the appropriate protein concentration for optimal stem cell attachment, which minimizes the need to calculate dilution schemes for every lot of matrix.

Reliable
Designed for 4°C storage for the life of the product, this matrix can be kept in the refrigerator along with your cell culture media—no more overnight thawing in clumsy ice baths and no risk of premature gelling. In addition, every lot is functionally qualified using human embryonic stem cells and tested to ensure it is free of lactose dehydrogenase elevating virus (LDEV), making it safe for all cell culture labs.

Cost effective
Geltrex hESC-Qualified Ready-To-Use matrix eliminates multiple steps—no more freezing, thawing, diluting, and aliquoting. This product is a true time saver.