Shop All Hybridoma Media

Cell MAB Medium (Gibco™)

Cell MAb Medium is formulated to produce high-yield monoclonal antibody secretion. It shows an increase in yield of 5–25 times more antibody than with conventional media. The amount of increased expression will be cell line dependent. Cell MAb Medium will not only increase yield, but also dramatically reduce media consumption and labor cost. Cells will retain viability for longer periods of time and require much less handling than is needed with traditional media.

Cell MAb Medium will support the growth of a wide variety of myeloma fusion partners and hybridomas, including Sp2/0, NS-1, P3X63Ag9, and FOX-NY, as well as other secreting cell lines such as CHO. It is HEPES based and can be used in either CO2 incubators or non-CO2 incubators in a closed system.

Hybridoma-SFM (Gibco™)

Hybridoma-SFM is a serum-free, very low-protein medium specifically developed to support the growth of hybridomas for antibody production. Hybridoma-SFM features:

• Ability to support suspension and stationary hybridomas and myelomas
• Complete, ready-to-use formulation
• Serum-free, very low-protein formulation

Ability to support suspension and stationary hybridomas and myelomas
Suitable for the culture of recombinant myeloma lines as well as traditional hybridomas, Hybridoma-SFM provides high cell densities and IgG production. Cholesterol-dependent cultures such as NS0 require the addition of 250X Cholesterol Lipid Concentrate.

Complete, ready-to-use formulation
Hybridoma-SFM is a complete medium that is ready to use. No supplementation is required.

Serum-free, very low-protein formulation
Hybridoma-SFM is a serum-free, very low-protein formulation, allowing for easier purification of your protein of interest. Hybridoma-SFM contains <20 µg/mL total protein.

Product use
Customers using Gibco® Hybridoma-SFM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP manufacturing and quality system
Hybridoma-SFM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

CD Hybridoma Medium (Gibco™)

CD Hybridoma Medium is an animal origin–free, chemically defined, protein-free medium specifically developed for the ability to support the growth of hybridomas for antibody production. Gibco® CD Hybridoma Medium features:

• Ability to support suspension and stationary hybridomas and myelomas
• Formulated without L-glutamine for stability
• Animal origin–free, protein-free, and chemically defined formulation

Ability to support suspension and stationary hybridomas and myelomas
Suitable for the culture of recombinant myeloma lines as well as traditional hybridomas, Gibco® CD Hybridoma Medium provides high cell densities and IgG production. Cholesterol-dependent cultures such as NS0 require the addition of 250X Cholesterol Lipid Concentrate.

Formulated without L-glutamine for stability
Gibco® CD Hybridoma Medium requires the addition of 8 mM L-glutamine or GlutaMAX™ supplement before use. GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.

Animal origin–free, protein-free, and chemically defined formulation
Gibco® CD Hybridoma Medium is animal origin–free, protein-free, and chemically defined, allowing for easier purification of your protein of interest. Gibco® chemically defined media contain no proteins, hydrolysates, or components of unknown composition.

Product use
Customers using Gibco® CD Hybridoma Medium in a manufacturing process, who have a submission with the FDA, may request from us a letter of authorization to reference our Type II Drug Master File (DMF).

cGMP manufacturing and quality system
Gibco® CD Hybridoma Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

CD Hybridoma AGT™ Medium (Gibco™)

Gibco® CD Hybridoma Medium is an animal origin-free, chemically-defined, protein-free medium specifically developed for the ability to support the growth of hybridomas for antibody production. Gibco® CD Hybridoma Medium features:
• Ability to support suspension and stationary hybridomas and myelomas
• Formulated without L-glutamine for stability
• Animal origin-free, protein-free and chemically defined formulation
• Convenient, easy-to-use Advanced Granulation Technology (AGT™) format

Ability to support suspension and stationary hybridomas and myelomas
Suitable for the culture of recombinant myeloma lines as well as traditional hybridomas, Gibco® CD Hybridoma Medium provides high cell densities and IgG production. Cholesterol-dependent cultures such as NS0 require the addition of 250x Cholesterol Lipid Concentrate.

Formulated without L-glutamine for stability
Gibco® CD Hybridoma Medium requires the addition of 8 mM L-glutamine or GlutaMAX™ supplement before use. GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.

Animal origin-free, protein-free and chemically defined formulation
Gibco® CD Hybridoma Medium is animal origin-free, protein-free and chemically defined, allowing for easier purification of your protein of interest. Gibco® chemically defined media contain no proteins, hydrolysates or components of unknown composition.

Convenient, easy-to-use Advanced Granulation Technology (AGT™) format
Our Advanced Granulation Technology™ (AGT™) format is an easy-to-use dry powder format. The granules dissolve instantly and pH-adjusts for fast media preparation. Complete reconstitution instructions are available in the product manual.

Product Use
For research use/further cell culture manufacturing. Not for use in diagnostic procedures. Customers using Gibco® CD Hybridoma Medium in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP Manufacturing and Quality System
Gibco® CD Hybridoma Medium is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

CD Hybridoma AGT™ Medium (Gibco™)

Gibco® CD Hybridoma Medium is an animal origin-free, chemically-defined, protein-free medium specifically developed for the ability to support the growth of hybridomas for antibody production. Gibco® CD Hybridoma Medium features:
• Ability to support suspension and stationary hybridomas and myelomas
• Formulated without L-glutamine for stability
• Animal origin-free, protein-free and chemically defined formulation
• Convenient, easy-to-use Advanced Granulation Technology (AGT™) format

Ability to support suspension and stationary hybridomas and myelomas
Suitable for the culture of recombinant myeloma lines as well as traditional hybridomas, Gibco® CD Hybridoma Medium provides high cell densities and IgG production. Cholesterol-dependent cultures such as NS0 require the addition of 250x Cholesterol Lipid Concentrate.

Formulated without L-glutamine for stability
Gibco® CD Hybridoma Medium requires the addition of 8 mM L-glutamine or GlutaMAX™ supplement before use. GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.

Animal origin-free, protein-free and chemically defined formulation
Gibco® CD Hybridoma Medium is animal origin-free, protein-free and chemically defined, allowing for easier purification of your protein of interest. Gibco® chemically defined media contain no proteins, hydrolysates or components of unknown composition.

Convenient, easy-to-use Advanced Granulation Technology (AGT™) format
Our Advanced Granulation Technology™ (AGT™) format is an easy-to-use dry powder format. The granules dissolve instantly and pH-adjusts for fast media preparation. Complete reconstitution instructions are available in the product manual below.

Product Use
For research use/further cell culture manufacturing. Not for use in diagnostic procedures. Customers using Gibco® CD Hybridoma Medium in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP Manufacturing and Quality System
Gibco® CD Hybridoma Medium is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Medium NCTC-109 (1X) (Gibco™)

NCTC-109 medium is a chemically defined medium that was originally developed by the National Cancer Institute and formulated for the culture of mouse Strain L sublines, NCTC clone 929 (L929 cells) in a serum-free environment. Gibco® NCTC-109 medium is also suitable for generating and maintaining hybridoma cells.
This NCTC-109 is modified as follows:
WithWithout
• Phenol Red• L-glutamine
• Coenzymes• HEPES

The complete formulation is available.

Gibco® NCTC-109 medium is rich in nucleosides, vitamins, coenzymes, reducing agents and other unique ingredients.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco® NCTC-109 medium is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco® NCTC-109 product made in our Scotland facility (21340-054). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

NCTC-109 contains no proteins, lipids, or growth factors. NCTC-109 medium typically requires supplementation of a growth factor cocktail (for serum free growth) or Fetal Bovine Serum (FBS). NCTC-109 medium uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

PFHM-II Protein-Free Hybridoma Medium (powder) (Gibco™)

Gibco® Protein Free Hybridoma Medium II (PFHM II) is a serum-free, protein-free medium specifically developed for the ability to support the growth of hybridomas for antibody production. Gibco® PFHM II features:
• Ability to support suspension and stationary hybridomas and myelomas
• Complete, ready-to-use formulation
• Serum-free, protein-free formulation
• Provided in a convenient easy-to-reconstitute powder format

Ability to support suspension and stationary hybridomas and myelomas
Suitable for the culture of recombinant myeloma lines as well as traditional hybridomas, Gibco® PFHM II provides high cell densities and IgG production. Cholesterol-dependent cultures such as NS0 require the addition of 250x Cholesterol Lipid Concentrate.

Complete, ready-to-use formulation
Once reconstituted, Gibco® PFHM II is a complete medium that is ready-to-use. No supplementation is required.

Serum-free, very low-protein formulation
Gibco® PFHM II is a serum-free, protein-free formulation, allowing for easier purification of your protein of interest.

Product Use
For Research Use Only. Not for use in diagnostic procedures.

cGMP Manufacturing and Quality System
Gibco® PFHM II is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

PFHM-II Protein-Free Hybridoma Medium (liquid) (Gibco™)

Gibco® Protein Free Hybridoma Medium II (PFHM II) is an animal origin-free, serum-free, protein-free medium specifically developed for the ability to support the growth of hybridomas for antibody production.

Gibco® PFHM II Features:

• Ability to support suspension and stationary hybridomas and myelomas
• Complete, ready-to-use formulation
• Animal origin-free, serum-free, protein-free formulation

Ability to support suspension and stationary hybridomas and myelomas
Suitable for the culture of recombinant myeloma lines as well as traditional hybridomas, Gibco® PFHM II provides high cell densities and IgG production. Cholesterol-dependent cultures such as NS0 require the addition of 250X Cholesterol Lipid Concentrate.

Complete, ready-to-use formulation
Gibco® PFHM II is a complete medium that is ready-to-use. No supplementation is required.

Animal origin-free, serum-free, protein-free formulation
Gibco® PFHM II is an animal origin-free, serum-free, protein-free formulation, allowing for easier purification of your protein of interest.

Product Use
For Research Use Only. Not for use in diagnostic procedures.

cGMP Manufacturing and Quality System
Gibco® PFHM II is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable Gibco® PFHM II product made in our Scotland facility (12040-051). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Hybridoma-SFM, powder (Gibco™)

Gibco® Hybridoma SFM is a serum-free, very low-protein medium specifically developed for the ability to support the growth of hybridomas for antibody production. Gibco® Hybridoma SFM features:
• Ability to support suspension and stationary hybridomas and myelomas
• Complete, ready-to-use formulation
• Serum-free, very low-protein formulation

Ability to support suspension and stationary hybridomas and myelomas
Suitable for the culture of recombinant myeloma lines as well as traditional hybridomas, Gibco® Hybridoma SFM provides high cell densities and IgG production. Cholesterol-dependent cultures such as NS0 require the addition of 250x Cholesterol Lipid Concentrate.

Complete, ready-to-use formulation
Once reconstituted, Gibco® Hybridoma SFM, powder is a complete medium that is ready-to-use. No supplementation is required.

Serum-free, very low-protein formulation
Gibco® Hybridoma SFM is a serum-free, very low-protein formulation, allowing for easier purification of your protein of interest. Gibco® Hybridoma SFM contains < 20 µg/ml total protein.

Product Use
For Research Use Only or Further Manufacturing. Not for use in diagnostic procedures. Customers using Gibco® Hybridoma SFM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP Manufacturing and Quality System
Gibco® Hybridoma SFM is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.