Shop All Neuronal Cell Media

B-27™ Plus Neuronal Culture System (Gibco™)

The B-27 Plus Neuronal Culture System is a next-generation media system that provides the highest rate of in vitro survival of primary rodent and human stem cell-derived neurons. Comprised of B-27 Plus Supplement (50X) and Neurobasal Plus Medium, this system is an evolution of the most cited neuronal cell culture products, B-27 Supplement and Neurobasal Medium. It features an optimized formulation, upgraded manufacturing process, and more stringent quality control of raw materials and final product. These improvements enable the highest neuronal cell survival, improved electrophysiological activity, and enhanced functional maturity versus other neuronal cell culture media systems.

The B-27 Plus Neuronal Culture System offers:
• At least 50% higher survival rate of primary rat neurons compared to classic B-27 Supplement and Neurobasal Medium
Highest survival rate of primary rat, mouse, and human stem cell-derived neurons compared to other commercially available serum-free neuronal media systems
Improved electrophysiological activity and maturation—more consistent, stable, and synchronized spontaneous activity in primary rodent neurons after 10 days compared to BrainPhys™ Neuronal Medium
Accelerates neurite outgrowth and increases the number of synaptic-positive puncta

Available as a 50X, 10 mL supplement with 500 mL basal medium, the B-27 Plus Neuronal Culture System can seamlessly replace other neuronal cell culture systems, such as classic B-27 Supplement and Neurobasal Medium. The individual components of the system are also available for purchase separately.

Hibernate™-E Medium (Gibco™)

Hibernate Medium is a CO2-independent nutrient medium for the maintenance of neural cells, tissue, and tissue slices. Hibernate-E Medium is optimized for embryonic cells or tissue in ambient carbon dioxide levels.

Hibernate Medium enables neurons to be maintained in ambient CO2 levels for up to 48 hours. This provides scientists with greater convenience and control while running bench-top live cell experiments, such as microscopy, flow cytometry, and other physiology studies. Hibernate Medium also allows for the preservation of viable brain tissue before processing for up to a month with refrigeration and maintenance of tissue slices.

Hibernate Medium may be combined with B-27 Supplements to yield a complete medium.

For other applications, we provide alternative formulations of Hibernate Medium and B-27 Supplements.

For established protocols using Hibernate Medium, access our Gibco Neurobiology Protocol Handbook.

View publications using Gibco neurobiology products at our Gibco CellCite.

Astrocyte Medium (Gibco™)

Astrocyte Medium has been specially formulated to support the growth of primary human and rat astrocytes while retaining their phenotypes. The medium has been internally validated and optimized to provide an optimal balance between cell growth and the maintenance of cell viability, normal phenotype, and function. If more rapid proliferation is desired, addition of an appropriate amount of epidermal growth factor (EGF) is recommended, although cells may exhibit morphological and phenotypic changes.

Astrocytes are a type of glial cell of the nervous system with a typical star shape. It has been shown in recent years that astrocytes not only function as gap fillers and structural supports, but play an active role in supporting the normal functions of neurons, oligodendrocytes, neural stem cells, and blood-brain barrier. They regulate the extracellular ionic and chemical environment, respond to CNS injury, and play a fundamental role in the pathogenesis of ischemic neuronal death, and they have remained one of the most active research areas in neuroscience.

Astrocyte Medium has three components: base medium (DMEM), N-2 Supplement, and OneShot Fetal Bovine Serum (FBS). Mixing of these three components yields a complete medium.

For established protocols using Astrocyte Medium, access our Gibco Neurobiology Protocol Handbook.

View publications using Gibco neurobiology products at our Gibco CellCite.

Neurobasal™ Plus Medium (Gibco™)

Neurobasal Plus Medium is the next-generation of one of the most cited neuronal cell culture products, Neurobasal Medium. It is a neuronal basal medium that provides the highest rates of in vitro survival of primary rodent and human stem cell-derived neurons when used with B-27 Plus Supplement. Neurobasal Plus Medium features an optimized formulation, upgraded manufacturing process, and more stringent quality control of raw materials and final product. These improvements enable the highest rates of neuronal cell survival, improved electrophysiological activity, and enhanced functional maturity versus other neuronal cell culture media systems.

Neurobasal Plus Medium, when combined with B-27 Plus Supplement, offers:
• At least 50% higher survival rate of primary rat neurons compared to classic Neurobasal Medium and B-27 Supplement
• Highest survival rate of primary rat, mouse, and human stem cell-derived neurons compared to other commercially available serum-free neuronal media systems
• Improved electrophysiological activity and maturation—more consistent, stable, and synchronized spontaneous activity in primary rodent neurons after 10 days compared to BrainPhys™ Neuronal Medium
• Accelerates neurite outgrowth and increases the number of synaptic-positive puncta

Available as a 500 mL basal medium, Neurobasal Plus Medium can seamlessly replace other neuronal cell culture media, such as classic Neurobasal Medium. The B-27 Plus Neuronal Culture System is also available for purchase, which is comprised of B-27 Plus Supplement and Neurobasal Plus Medium, offering a convenient and valuable all-in-one ordering solution.

Neurobasal™-A Medium, minus phenol red (Gibco™)

Neurobasal Medium minus phenol red is a basal medium designed for long-term maintenance and maturation of pure post-natal and adult brain neurons without the need for an astrocyte feeder layer when used with Gibco B-27 supplements. Use this modified formulation of Neurobasal Medium when the presence of phenol red will interfere with your results.

Neurobasal Medium is suitable for use in most neuronal cell applications. For other applications, we provide alternative formulations of Neurobasal media and B-27 supplements.

For established protocols using Neurobasal media, access our Gibco Neurobiology Protocol Handbook.

View publications using Gibco neurobiology products at our Gibco CellCite.

Neurobasal™-A Medium (Gibco™)

Neurobasal-A Medium is a basal medium designed for long-term maintenance and maturation of pure post-natal and adult brain neurons without the need for an astrocyte feeder layer when used with Gibco B-27 supplements.

Neurobasal Medium is suitable for use in most neuronal cell applications. For other applications, we provide alternative formulations of Neurobasal media and B-27 supplements.

For established protocols using Neurobasal media, access our Gibco Neurobiology Protocol Handbook.

View publications using Gibco neurobiology products at our Gibco CellCite.

Gibco™ Neural Cell Culture Starter Kit (Gibco™)

The Gibco Neural Cell Culture Starter Kit provides researchers with a convenient package of reagents for the culture of a wide variety of neural cell types.

Whether you are culturing neural cell lines, primary neurons, or neural stem cells, having the right components for your complete neural cell culture medium is essential to achieving the best cell culture results.

This starter kit includes the following products for neural cell culture:
• B-27 Serum-free Supplement (50X), 10 mL, Cat. No. 17504044
• GlutaMAX Supplement, 100 mL, Cat. No. 35050061
• Neurobasal Medium, 500 mL, Cat. No. 21103049

Neurobasal™-A Medium, no D-glucose, no sodium pyruvate (Gibco™)

Neurobasal-A medium without D-glucose and sodium pyruvate is used to support the growth of post-natal CNS neurons in applications where the presence of D-glucose and sodium pyruvate can cause interference.

For neural studies that require the absence of insulin, glucose, and sodium pyruvate, such as glucose response or insulin response studies, we recommend Gibco B-27 Supplement Minus Insulin as a supplement to this medium.

Neurobasal Medium is suitable for use in most neuronal cell applications. For other applications, we provide alternative formulations of Neurobasal media and B-27 supplements.

For established protocols using Neurobasal media, access our Gibco Neurobiology Protocol Handbook.

View publications using Gibco neurobiology products at our Gibco CellCite.

Hibernate™-A Medium (Gibco™)

Hibernate Medium is a CO2-independent nutrient medium for the maintenance of neural cells, tissue, and tissue slices. Hibernate-A Medium is optimized for adult and postnatal cells or tissue in ambient carbon dioxide levels.

Hibernate Medium enables neurons to be maintained in ambient CO2 levels for up to 48 hours. This provides scientists with greater convenience and control while running bench-top live cell experiments, such as microscopy, flow cytometry, and other physiology studies. Hibernate Medium also allows for the preservation of viable brain tissue before processing for up to a month with refrigeration and maintenance of tissue slices.

Hibernate Medium may be combined with B-27 Supplements to yield a complete medium.

For other applications, we provide alternative formulations of Hibernate Medium and B-27 Supplements.

For established protocols using Hibernate Medium, access our Gibco Neurobiology Protocol Handbook.

View publications using Gibco neurobiology products at our Gibco CellCite.

B-27™ Plus Supplement (50X) (Gibco™)

B-27 Plus Supplement is the next-generation of the most cited neuronal cell culture product, B-27 Supplement. It is a serum-free supplement that provides the highest rate of in vitro survival of primary rodent and human stem cell-derived neurons when used with Neurobasal Plus Medium. B-27 Plus Supplement features an optimized formulation, upgraded manufacturing process, and more stringent quality control of raw materials and final product. These improvements enable the highest rate of neuronal cell survival, improved electrophysiological activity, and enhanced functional maturity versus other neuronal cell culture media systems.

B-27 Plus Supplement, when combined with Neurobasal Plus Medium, offers:
• At least 50% higher survival rate of primary rat neurons compared to classic B-27 Supplement and Neurobasal Medium
Highest survival rate of primary rat, mouse, and human stem cell-derived neurons compared to other commercially available serum-free neuronal media systems
Improved electrophysiological activity and maturation—more consistent, stable, and synchronized spontaneous activity in primary rodent neurons after 10 days compared to BrainPhys™ Neuronal Medium
Accelerates neurite outgrowth and increases the number of synaptic-positive puncta

Available as a 50X 10-mL supplement, B-27 Plus Supplement can seamlessly replace other neuronal cell culture supplements, such as classic B-27 Supplement. The B-27 Plus Neuronal Culture System is also available for purchase, which is comprised of B-27 Plus Supplement and Neurobasal Plus Medium, offering a convenient and valuable all-in-one ordering solution.

B-27™ Electrophysiology Kit (Gibco™)

The B-27® Electrophysiology Kit contains Neurobasal® Medium, Electro and B-27® Supplement, Electro, both specifically developed to enhance network spike rates in cultured neurons when compared with other media and supplement combinations. Electrophysiology is the fundamental technique to assess such electrical activities to study the functions and dysfunctions in cultured neurons.

Using the B-27® Electrophysiology Kit in your neuronal studies offers:

• Increased synapse densities
• More spontaneous spike rates
• Improved multielectrode array efficiency

More Synapses, More Signal, Simpler Testing
The B-27® Electrophysiology Kit is an improvement to Neurobasal® and B-27® combination for cultured networks. Hippocampal and cortical neurons cultured in this media system show an increased density of synapses and neurotransmitter receptors which produce higher spike rates in neural networks, helping to simplifying your study.

Relevant Results for Your Electrophysiology Studies
Using Invitrogen® B-27® Electrophysiology Kit, allows you to get more relevant results when studying neuron physiology and the biochemical basis of normal and disease processes. The B-27® Electrophysiology Kit can yield better results in other electrophysiological studies, including neurons used in multielectrode arrays in neurotoxicology and neuropharmacology studies.

For other applications, we offer alternative formulations of B-27® and Neurobasal®.

Neurobasal™ Medium, minus phenol red (Gibco™)

Neurobasal Medium, minus phenol red is a basal medium designed for long-term maintenance and maturation of pure pre-natal and embryonic neurons without the need for an astrocyte feeder layer when used with Gibco B-27 supplements. Use this modified formulation of Neurobasal Medium when the presence of phenol red will interfere with your results.

Neurobasal Medium is suitable for use in most neuronal cell applications. For other applications, we provide alternative formulations of Neurobasal media and B-27 supplements.

For established protocols using Neurobasal media, access our Gibco Neurobiology Protocol Handbook.

View publications using Gibco neurobiology products at our Gibco CellCite.

Cell Therapy Systems Hibernate™-E CTS™ Medium (Gibco™)

CTS Hibernate-E Medium is a serum-free, nutrient basal medium for the short-term maintenance of embryonic human tissue in ambient CO2 conditions.

Quick and easy shipment of human tissues
CTS Hibernate-E Medium can be used for shipment of human embryonic tissue such as brain tumors, umbilical cords, and foreskin at ambient temperature using a simple three step process:

1) Collect tissue in a conical tube containing CTS Hibernate-E Medium (brain tumor cells require additional supplements).
2) Ship tissues in CTS Hibernate-E Medium within 24 to 72 hours on cold packs.
3) Transfer received tissues into medium of choice and initiate cultivation.

CTS quality
Gibco CTS products are manufactured at a site that uses methods and controls that conform with cGMP for medical devices, 21 CFR Part 820. Our FDA-registered manufacturing sites are ISO 13485– and ISO 9001–certified.

Accompanied by documentation to support your regulatory filing
Specific intended-use statements, full documentation traceability, and convenient access to our Drug Master File (DMF) are available.

CTS Hibernate-E Medium can be combined with CTS B-27 Supplements to yield a complete medium.

For other applications, we provide alternative formulations of CTS Hibernate Medium and CTS B-27 Supplements.

For established protocols using Hibernate Medium, access our Gibco Neurobiology Protocol Handbook.

View publications using Gibco neurobiology products at our Gibco CellCite.

CTS™ Neurobasal™ Medium (Gibco™)

CTS Neurobasal Medium is designed with the translational stem cell researcher in mind. It can be used for long-term maintenance and maturation of pure post-natal and adult brain neurons without the need for an astrocyte feeder layer when used with CTS B-27 supplements.

CTS quality
Gibco CTS products are manufactured at a site that uses methods and controls that conform with cGMP for medical devices, 21 CFR Part 820. Our FDA-registered manufacturing sites are ISO 13485– and ISO 9001–certified.

Accompanied by documentation to support your regulatory filing
Specific intended-use statements, full documentation traceability, and convenient access to our Drug Master File (DMF) are available.

For other applications, we provide alternative formulations of CTS Neurobasal media and CTS B-27 supplements.

For established protocols using Neurobasal media, access our Gibco Neurobiology Protocol Handbook.

View publications using Gibco neurobiology products at our Gibco CellCite.

Neurobasal™ Medium (Gibco™)

Neurobasal Medium is a basal medium designed for long-term maintenance and maturation of pure pre-natal and embryonic neuronal cell populations without the need for an astrocyte feeder layer when used with Gibco B-27 supplements.

Neurobasal Medium is suitable for use in most neuronal cell applications. For other applications, we provide alternative formulations of Neurobasal media and B-27 supplements.

For established protocols using Neurobasal media, access our Gibco Neurobiology Protocol Handbook.

View publications using Gibco neurobiology products at our Gibco CellCite.