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IPL-41 Insect Medium (1X) (Gibco™)

Gibco® IPL-41 Insect Medium was originally designed for the growth of Spodeptera frugiperda (Sf9) cells and the expression of recombinant Autographa Californica Nuclear Polyhedrosis Virus (rAcNPV) using the Baculovirus Expression Vector System (BEVS). We offer a variety of Gibco® Media options for a range of insect cell culture applications.

This IPL-41 Insect Medium is modified as follows:

WithWithout
• L-glutamine • Tryptose phosphate broth
• Sodium bicarbonate • Yeastolate

The complete formulation is available. IPL-41 Insect Medium contains unique components required for insect cell culture, including malic acid, fumaric acid, and alpha-ketoglutaric acid.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

Dual Site cGMP Manufacturing and Quality System
Gibco® IPL-41 Insect Medium is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable Gibco® IPL-41 Insect Medium product made in our Scotland facility (11405-057). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Gibco® IPL-41 Insect Medium is commonly supplemented with 10% Fetal Bovine Serum (FBS) before use. IPL-41 Insect Medium requires ambient CO2in order to maintain physiological pH.

Express Five™ SFM (Gibco™)

Express Five® SFM is a protein-free, serum-free medium specifically developed to support the long-term suspension growth of High Five™ (BTI-TN-5B1-4) cells in suspension for use in the Baculovirus Expression Vector System (BEVS). This medium requires the addition of 16 mM L-glutamine before use. Gibco® Express Five® SFM features:

• Superior long-term suspension growth of High Five™ cells
• Protein-free, serum-free formulation
• Optimized for recombinant protein production
• Scalable in the CelliGen® bioreactor and WAVE Bioreactor® System

Superior growth of High Five™ cells
Gibco® Express Five® SFM provides peak cell densities of up to 6 × 106 viable cells per mL in shaker cultures. Cells adapted to other commercially available serum-free media can be subcultured directly into Gibco® Express Five® SFM, usually without any further adaptation (see figure). Cells usually require adaptation from serum-containing formulations.

Protein-free, serum-free formulation
Gibco® Express Five® SFM is a protein-free and serum-free formulation, allowing for easier purification of your protein of interest.

Optimized for recombinant protein expression
Gibco® Express Five® SFM is optimized for high protein production using the Baculovirus Expression Vector System (BEVS). Using BEVS, we routinely achieve rβ-galactosidase protein expression of up to 1.7 U/cell, a significant improvement over other media (see figure)

Scalable in the CelliGen® bioreactor and WAVE Bioreactor® System
Protein production using Gibco® Express Five® SFM can be scaled up in bioreactors such as the 5 L CelliGen® bioreactor and the WAVE Bioreactor® System. The appropriate impeller or rocking speed and seeding density should be optimized for each system. Depending on the bioreactor design, it may be necessary to supplement Gibco® Express Five® SFM with additional Pluronic® F-68 to avoid sheer stress in the culture.

cGMP manufacturing and quality system
Gibco® Express Five® SFM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.