Shop All Sf9 & Sf21 Media

ExpiSf™ CD Medium (Gibco™)

ExpiSf CD Medium is a GMP-manufactured, chemically defined, yeastolate-free, serum-free, protein-free, animal origin-free medium specifically designed for high-density growth, transfection, and baculovirus infection of suspension-adapted Sf9 (Spodoptera frugiperda) insect cells.

ExpiSf CD Medium:
• Delivers high reproducibility and lot-to-lot reliability
• Facilitates high-level expression of baculovirus-infected cultures, achieving protein yields three times higher than traditional insect expression platforms
• Supports growth of suspension Sf9 cultures to densities of approximately 2 x 107 cells/mL, with >90% cell viability
• Supports high-density infection of suspension Sf9 cells with recombinant baculovirus
• Supports growth, transfection, and baculovirus infection of Sf9 cells in culture formats from 24-deep well plates to >2 L in shaker flasks
• Is a ready-to-use formulation, with no supplementation required

ExpiSf CD Medium is a core component of the ExpiSf Expression System. For highest level protein expression, the medium is used in combination with a matched protein expression enhancer, ExpiSf Enhancer, which is supplied as part of the ExpiSf Protein Production Kit. Sf21 cells adapted to other commercially-available serum-free media may be adapted for growth in ExpiSf CD Medium; although, sequential adaptation is required and high-density growth isn’t guaranteed. ExpiSf CD Medium is not recommended for adherent cell cultures. Cells are typically grown in Erlenmeyer-style flasks on a shaker platform in a non-humidified, non-CO2 cell culture incubator.

cGMP manufacturing
ExpiSf CD Medium is manufactured using an ISO 13485-certified process according to 21 CFR 820 medical device current good manufacturing practices (cGMP).

Dry media formulations
For added flexibility, ExpiSf CD Medium can be made in AGT dry format at large scale. For custom media requests, please contact Gibco Custom Media Services.

Sf-900™ II SFM, no methionine, no cystine (Gibco™)

Sf-900™ II SFM (without methionine and cystine) is a serum-free, protein-free insect cell culture medium optimized for the growth and maintenance of Spodoptera frugiperda (Sf9 and Sf21) cells and for large-scale production of recombinant proteins expressed using the baculovirus expression vector system (BEVS). This medium is suitable for suspension and monolayer culture methods and supports growth of other lepidopteran cell lines. Gibco® Sf-900™ II SFM features:

• Superior long-term, high-density growth
• Optimized for recombinant protein production
• Serum-free, protein-free, ready-to-use formulation
• Scalable in bioreactors

Superior long-term, high-density growth
Spodoptera frugiperda (Sf9) cells grown in Sf-900™ II SFM achieve maximum cell densities of 9 to 12 × 106 cells/mL, a significant improvement over competitors' formulations and Grace’s medium (see Product Manual). Increases in maximum cell densities of 20–100% are also observed with the Lymantria dispar (Gypsy moth) and Trichoplusia ni (Tn-368; Cabbage Looper) cell lines. Gibco® Sf-900™ II SFM is capable of supporting the cultures to >20 passages.

Optimized for recombinant protein production
Traditionally, Grace's medium supplemented with 10% FBS has been used for recombinant protein expression. Sf-900™ II SFM is an improved serum-free, protein-free medium designed for growth of Sf9 and other lepidopteran cell lines and production of insect virus and rDNA proteins.

Serum-free, protein-free, ready-to-use formulation
Gibco® Sf-900™ II SFM is a serum-free, protein-free medium that allows for much easier purification of your protein of interest. Sf-900™ II SFM is ready to use; it does not require addition of serum, glutamine, or surfactants. Cells adapted to other commercially available serum-free media can be subcultured directly into Sf-900™ II SFM, usually without any further adaptation. Cells usually require some adaptation from serum-containing formulations.

Scalable in bioreactors
The utility of Sf-900™ II SFM in larger-scale cell culture systems was demonstrated with a 5 L Celligen™ bioreactor. Successful infections with rAcNPV were carried out producing rβ-Gal and rEPO (see Product Manual).

Product use
Customers using Gibco® Sf-900 II SFM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP manufacturing and quality system
For supply chain continuity, we manufacture Gibco® Sf-900 II SFM at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.

Sf-900™ Medium (1.3X) (Gibco™)

Sf-900™ SFM is a complete serum-free, low protein insect cell culture medium developed for the convenient and reproducible formation of 1% agarose overlays used for plaque assays of baculovirus in Spodoptera frugiperda (Sf9 and Sf21) cells. When used with Gibco® 4% Agarose Gel, Sf900™ SFM (1.3x) allows for convenient formation of a 1% overlay with correct 1x final osmolality and nutrition required for insect cell growth during the plaque assay.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

cGMP Manufacturing and Quality System
Gibco® Sf-900 II SFM is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Wave Cellbag prefilled with Sf-900™ II Medium (custom product) (Gibco™)

Sf-900 II Serum Free Media, 10L, prefilled in a 20L Series-O Wave Cellbag. Sf-900 II SFM is a protein-free insect cell culture medium optimized for the growth and maintenance of Spodoptera frugiperda (Sf9 and Sf21) cells and for large-scale production of recombinant proteins expressed using the baculovirus expression vector system (BEVS). This medium is suitable for suspension and monolayer culture methods and supports growth of other lepidopteran cell lines.

TC-100 Insect Medium (Gibco™)

TC 100 Insect Medium was originally designed for the growth of insect cells, including Spodeptera frugiperda (Sf9), and the expression of recombinant Autographa Californica Nuclear Polyhedrosis Virus (rAcNPV) using the Baculovirus Expression Vector System (BEVS). We offer a variety of Gibco® Media options for a range of insect cell culture applications.

This IPL-41 Insect Medium is modified as follows:

With
• L-glutamine
• Tryptose phosphate broth
• Sodium bicarbonate

The complete formulation is available. TC 100 Insect Medium is rich in amino acids and uses tryptose as an additional sugar source.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

cGMP Manufacturing and Quality System
Gibco® TC 100 Insect Medium is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Gibco® TC 100 Insect Medium is commonly supplemented with 10% Fetal Bovine Serum (FBS) before use. TC 100 Insect Medium requires ambient CO2 in order to maintain physiological pH.

Sf-900™ II SFM (Gibco™)

Sf-900™ II SFM is a serum-free, protein-free insect cell culture medium optimized for the growth and maintenance of Spodoptera frugiperda (Sf9 and Sf21) cells and for large-scale production of recombinant proteins expressed using the baculovirus expression vector system (BEVS). This medium is suitable for suspension and monolayer culture methods and supports growth of other lepidopteran cell lines. Sf-900™ II SFM features:

• Superior long-term, high-density growth
• Optimized for recombinant protein production
• Serum-free, protein-free, ready-to-use formulation
• Scalable in bioreactors

Superior long-term, high-density growth
Spodoptera frugiperda (Sf9) cells grown in Sf-900™ II SFM achieve maximum cell densities of 9 to 12 x 106 cells/mL, a significant improvement over competitors' formulations and Grace’s medium (see Product Manual). Increases in maximum cell densities of 20–100% are also observed with the Lymantria dispar (Gypsy moth) and Trichoplusia ni (Tn-368; Cabbage Looper) cell lines. Sf-900™ II SFM is capable of supporting the cultures to >20 passages.

Optimized for recombinant protein production
Traditionally, Grace's medium supplemented with 10% FBS has been used for recombinant protein expression. Sf-900™ II SFM is an improved serum-free, protein-free medium designed for growth of Sf9 and other lepidopteran cell lines and production of insect virus and rDNA proteins.

Serum-free, protein-free, ready-to-use formulation
Sf-900™ II SFM is a serum-free, protein-free medium that allows for much easier purification of your protein of interest. Sf-900™ II SFM is ready to use; it does not require addition of serum, glutamine, or surfactants. Cells adapted to other commercially available serum-free media can be subcultured directly into Sf-900™ II SFM, usually without any further adaptation. Cells usually require some adaptation from serum-containing formulations.

Scalable in bioreactors
The utility of Sf-900™ II SFM in larger-scale cell culture systems was demonstrated with a 5 L Celligen™ bioreactor. Successful infections with rAcNPV were carried out producing rβ-Gal and rEPO (see Product Manual).

Product use
Customers using Gibco® Sf-900 II SFM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP manufacturing and quality system
For supply chain continuity, we manufacture Sf-900 II SFM at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.

Sf-900™ III SFM (Gibco™)

Sf-900™ III SFM is a low-hydrolysate, serum-free, protein-free, animal origin–free insect cell culture medium optimized for the growth and maintenance of Spodoptera frugiperda (Sf9 and Sf21) cells and for recombinant gene expression using the baculovirus and stable insect expression systems. This medium is suitable for suspension and monolayer culture methods and supports growth of other lepidopteran cell lines. Features of Gibco® Sf-900™ III SFM:

• Superior long-term, high-density growth
• Optimized for recombinant protein production
• Serum-free, protein-free, animal origin–free, ready-to-use formulation
• Improved lot-to-lot consistency

Superior long-term, high-density growth
Spodoptera frugiperda (Sf9) cells grown in Sf-900™ III SFM achieve maximum cell densities of 10 to 14 × 106 cells/mL, a significant improvement over Sf-900™ II SFM and other commercially available serum-free media (see figure).

Optimized for recombinant protein production
Traditionally, Grace's medium supplemented with 10% FBS has been used for recombinant protein expression. Sf-900™ III SFM is an improved serum-free, protein-free, animal origin–free medium designed for growth of Sf9 and other lepidopteran cell lines and production of insect viruses and recombinant proteins.

Serum-free, protein-free, animal origin–free, ready-to-use formulation
Gibco® Sf-900™ III SFM is a serum-free, protein-free, animal origin–free medium that allows for much easier purification of your protein of interest. Sf-900™ III SFM is ready to use; it does not require addition of serum, glutamine, or surfactants. Cells adapted to other commercially available serum-free media should be sequentially adapted into Sf-900™ III SFM (see product manual for details).

Improved lot-to-lot consistency
Gibco® Sf-900™ III SFM contains reduced hydrolysate concentrations, improving lot-to-lot consistency over Sf-900™ II SFM (see figure).

cGMP manufacturing and quality system
Gibco® Sf-900 II SFM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

TC-100 Insect Medium, powder (Gibco™)

TC 100 Insect Medium was originally designed for the growth of insect cells, including Spodeptera frugiperda (Sf9), and the expression of recombinant Autographa Californica Nuclear Polyhedrosis Virus (rAcNPV) using the Baculovirus Expression Vector System (BEVS). We offer a variety of Gibco® Media options for a range of insect cell culture applications.

This IPL-41 Insect Medium is made as follows:

WithWithout
• L-glutamine • Sodium bicarbonate
• Tryptose phosphate broth

The complete formulation is available. TC 100 Insect Medium is rich in amino acids and uses tryptose as an additional sugar source.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

cGMP Manufacturing and Quality System
Gibco® TC 100 Insect Medium is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Gibco® TC 100 Insect Medium is commonly supplemented with 10% Fetal Bovine Serum (FBS) before use. TC 100 Insect Medium requires ambient CO2 in order to maintain physiological pH. The powder form of TC 100 Insect Medium requires the addition of 350 mg/L sodium bicarbonate before use.