Shop All Epidermal Cells

Human Epidermal Keratinocytes, neonatal, Animal Product-Free (HEKn-APF) (Gibco™)

Primary human epidermal keratinoctyes from neonatal foreskin. Isolated, grown and cryopreserved at the end of the primary culture in an animal product-free environment.

• Viability at least 70%
• ≥500,000 viable cells⁄vial
• Capable of at least 30 population doublings
• Mycoplasma: Not Detected
• Hepatitis B: Not Detected
• Hepatitis C: Not Detected
• HIV-1: Not Detected
• Bacteria, yeast, and other fungi: Not Detected

Human Epidermal Melanocytes, neonatal, darkly pigmented donor, (HEMn-DP), Proliferating Culture, Prepared in Medium 254 and HMGS (Gibco™)

Tertiary cultures established from cryopreserved cells, grown to approximately 50% confluence in T-25 flasks.

Human Epidermal Keratinocytes, adult, Animal Product-Free (HEKa-APF) (Gibco™)

Primary human epidermal keratinoctyes from adult skin. Isolated, grown and cryopreserved at the end of the primary culture in an animal product-free environment.

• Viability at least 70%
• ≥500,000 viable cells⁄vial
• Capable of at least 25 population doublings
• Mycoplasma: Not Detected
• Hepatitis B: Not Detected
• Hepatitis C: Not Detected
• HIV-1: Not Detected
• Bacteria, yeast, and other fungi: Not Detected

Human Epidermal Melanocytes, neonatal, darkly pigmented donor, (HEMn-DP) (Gibco™)

Primary human epidermal melanocytes isolated from neonatal foreskin, cryopreserved at the end of the secondary culture. These cells are isolated from darkly pigmented tissue.
• Viability at least 70%
• ≥500,000 viable cells/vial
• Capable of at least 12 population doublings
• Mycoplasma: Not Detected
• Hepatitis B: Not Detected
• Hepatitis C: Not Detected
• HIV-1: Not Detected
• Bacteria, yeast, and other fungi: Not Detected

Human Epidermal Keratinocytes, neonatal (HEKn) (Gibco™)

Primary human epidermal keratinocytes isolated from neonatal foreskin, cryopreserved at the end of the primary culture.
• Viability at least 70%
• ≥500,000 viable cells/vial
• Capable of at least 30 population doublings
• Mycoplasma: Not Detected
• Hepatitis B: Not Detected
• Hepatitis C: Not Detected
• HIV-1: Not Detected
• Bacteria, yeast, and other fungi: Not Detected

Human Epidermal Melanocytes, adult, lightly pigmented donor, (HEMa-LP) (Gibco™)

Primary human epidermal melanocytes isolated from adult skin, cryopreserved at the end of the secondary culture. These cells are isolated from lightly pigmented tissue.
• Viability at least 70%
• ≥500,000 viable cells/vial
• Capable of at least 16 population doublings
• Mycoplasma: Not Detected
• Hepatitis B: Not Detected
• Hepatitis C: Not Detected
• HIV-1: Not Detected
• Bacteria, yeast, and other fungi: Not Detected
• Positive for Mel-5 antigen in the fourth culture after thawing

Human Epidermal Melanocytes, neonatal, lightly pigmented donor, (HEMn-LP) (Gibco™)

Primary human epidermal melanocytes isolated from neonatal foreskin, cryopreserved at the end of the secondary culture. These cells are isolated from lightly pigmented tissue.
• Viability at least 70%
• ≥500,000 viable cells/vial
• Capable of at least 16 population doublings
• Mycoplasma: Not Detected
• Hepatitis B: Not Detected
• Hepatitis C: Not Detected
• HIV-1: Not Detected
• Bacteria, yeast, and other fungi: Not Detected"

Human Epidermal Keratinocytes, adult (HEKa) (Gibco™)

Primary human epidermal keratinocytes isolated from adult skin, cryopreserved at the end of the primary culture.
• Viability at least 70%
• ≥500,000 viable cells/vial
• Capable of at least 25 population doublings
• Mycoplasma: Not Detected
• Hepatitis B: Not Detected
• Hepatitis C: Not Detected
• HIV-1: Not Detected
• Bacteria, yeast, and other fungi: Not Detected

Human Epidermal Keratinocytes, Pooled (HEKp) (Gibco™)

Human Epidermal Keratinocytes, pooled (HEKp) from Invitrogen are normal cells isolated from multiple neonatal foreskins. These cells are optimal for investigating the molecular and biochemical bases of normal and disease processes of the skin. They have been cryopreserved at the end of the primary culture stage in animal origin-free media containing 10% DMSO. Use HEKp for your research into fibrosis, wound healing, irritancy, and diseases such as psoriasis.
Guaranteed to have ≥70% viability upon thawing
• Each vial guaranteed to reach ≥15 population doublings after thaw
• Sufficient cells to seed ≥100 cm2
• Tested for potential biological contaminants
• Optimized for use in EpiLife® serum-free media

Get More Physiologically Relevant Results with Invitrogen Primary Cells

Human primary cell cultures closely mimic the in vivo state and thus generate more physiologically relevant data. Our Human Epidermal Keratinocytes are isolated and pooled from 4-6 neonatal foreskins. This process is robust and yields large donor lots to ensure availability of consistent product for your studies.

Reliable Performance and Purity
Primary cells from Invitrogen are guaranteed to have ≥70% viability upon thawing with each vial containing sufficient cells to seed ≥100 cm2 of surface area. Performance testing guarantees each vial of HEKp will reach ≥15 population doublings after thaw. In addition, each lot is tested for potential biological contaminants--HIV-1, Hepatitis B, Hepatitis C viruses, mycoplasma, bacteria, yeast, and other fungi-- to ensure quality and robustness.

Optimized for Use with Gibco® Media
Human Epidermal Keratinocytes, pooled, from Invitrogen have been optimized for use with our serum-free media, specifically EpiLife® Medium with 60 µM calcium and Human Keratinocyte Growth Supplement (HKGS). Preparation of supplemented media is a simple process; the final solution can be stored for up to 30 days at 4°C.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Learn more about our complete system of media and supplements for culturing Human Epidermal Keratinocytes

Human Epidermal Melanocytes, neonatal, moderately pigmented donor, (HEMn-MP) (Gibco™)

Primary human epidermal melanocytes isolated from neonatal foreskin, cryopreserved at the end of the secondary culture. These cells are isolated from moderately pigmented tissue.
• Viability at least 70%
• ≥500,000 viable cells/vial
• Capable of at least 12 population doublings
• Mycoplasma: Not Detected
• Hepatitis B: Not Detected
• Hepatitis C: Not Detected
• HIV-1: Not Detected
• Bacteria, yeast, and other fungi: Not Detected