Avidin, Streptavidin & Biotin
Streptavidin - AP (Invitrogen™)
Reactivity: AP-SA reacts with biotin.
Applications: Immunohistochemistry, Western blotting, and ELISA
Applications: Immunohistochemistry, Western blotting, and ELISA
Streptavidin - HRP (Invitrogen™)
Reactivity: HRP-SA reacts with biotin.
Applications: Immunohistochemistry, Western blotting, and ELISA
Applications: Immunohistochemistry, Western blotting, and ELISA
Streptavidin, Unconjugated (Invitrogen™)
Purified from Streptomyces avidinii and provided in lyophilized form. The streptavidin/avidin-biotin staining and detection strategy is used when increased assay sensitivity is desired for a wide variety of systems.
Format/Formulation: Unconjugated
Format/Formulation: Unconjugated
eBioscience™ Streptavidin PE (Invitrogen™)
The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Streptavidin binds to biotin with high affinity.
Laser
Blue Laser, Green Laser, Yellow-Green Laser
Emit
578 nm
Excite
488 - 561 nm
Reported Application
Flow Cytometric Analysis
Laser
Blue Laser, Green Laser, Yellow-Green Laser
Emit
578 nm
Excite
488 - 561 nm
Reported Application
Flow Cytometric Analysis
Streptavidin, Alexa Fluor™ 633 conjugate (Invitrogen™)
Alexa Fluor® 633 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor® dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays. Alexa Fluor® dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.
Important Features of Alexa Fluor® 633 Streptavidin Conjugates:
• Alexa Fluor® 633 streptavidin conjugate has Ex/Em maxima of ~ (632/647)
• Bright, photostable fluorescence
• High solubility in aqueous solutions
• Available in multiple colors
• Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more
Properties of Alexa Fluor® Dyes
Alexa Fluor® dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.
Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Related Links:
Learn more about Avidin-Biotin Detection
Learn more about Alexa Fluor® Dyes
Find out about other Labeled Streptavidin Conjugates
Read Avidin and Streptavidin Conjugates-Section 7.6 in the Molecular Probes Handbook
Important Features of Alexa Fluor® 633 Streptavidin Conjugates:
• Alexa Fluor® 633 streptavidin conjugate has Ex/Em maxima of ~ (632/647)
• Bright, photostable fluorescence
• High solubility in aqueous solutions
• Available in multiple colors
• Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more
Properties of Alexa Fluor® Dyes
Alexa Fluor® dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.
Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Related Links:
Learn more about Avidin-Biotin Detection
Learn more about Alexa Fluor® Dyes
Find out about other Labeled Streptavidin Conjugates
Read Avidin and Streptavidin Conjugates-Section 7.6 in the Molecular Probes Handbook
Streptavidin HRP (Invitrogen™)
Purified from Streptomyces avidinii and provided in a phosphate buffered saline solution. The streptavidin/avidin-biotin staining and detection strategy is used when increased assay sensitivity is desired for a wide variety of systems.
Format/Formulation: HRP Conjugate
Format/Formulation: HRP Conjugate
Streptavidin, Alexa Fluor™ 660 conjugate (Invitrogen™)
Alexa Fluor® 660 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor® dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays. Alexa Fluor® dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.
Important Features of Alexa Fluor® 660 Streptavidin Conjugates:
• Alexa Fluor® 660 streptavidin conjugate has Ex/Em maxima of ~ (663/690)
• Bright, photostable fluorescence
• High solubility in aqueous solutions
• Available in multiple colors
• Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more
Properties of Alexa Fluor® Dyes
Alexa Fluor® dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.
Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Related Links:
Learn more about Avidin-Biotin Detection
Learn more about Alexa Fluor® Dyes
Find out about other Labeled Streptavidin Conjugates
Read Avidin and Streptavidin Conjugates-Section 7.6 in the Molecular Probes Handbook
Important Features of Alexa Fluor® 660 Streptavidin Conjugates:
• Alexa Fluor® 660 streptavidin conjugate has Ex/Em maxima of ~ (663/690)
• Bright, photostable fluorescence
• High solubility in aqueous solutions
• Available in multiple colors
• Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more
Properties of Alexa Fluor® Dyes
Alexa Fluor® dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.
Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Related Links:
Learn more about Avidin-Biotin Detection
Learn more about Alexa Fluor® Dyes
Find out about other Labeled Streptavidin Conjugates
Read Avidin and Streptavidin Conjugates-Section 7.6 in the Molecular Probes Handbook
Avidin, Egg White (Invitrogen™)
Avidin is a glycosylated biotin-binding protein that has a basic isoelectric point and is widely used to detect biotinylated probes.
Streptavidin R PE (Invitrogen™)
Purified from Streptomyces avidinii and provided in a phosphate buffered saline solution. The streptavidin/avidin-biotin staining and detection strategy is used when increased assay sensitivity is desired for a wide variety of systems.
Format/Formulation: RPE Conjugate
Format/Formulation: RPE Conjugate
Streptavidin Protein, HRP (Thermo Scientific™)
Thermo Scientific™ Pierce™ Streptavidin, Horseradish Peroxidase Conjugate includes streptavidin in a purified form conjugated to peroxidase for substrate-based detection.
Streptavidin, Horseradish Peroxidase Conjugate enables detection of biotinylated antibodies and other probes in a variety of standard assay methods, including western blotting, ELISA, immunohistochemistry, and fluorescence imaging. Streptavidin, Horseradish Peroxidase conjugate is supplied as lyophilized powder in phosphate-based buffer for immediate reconstitution with water.
Streptavidin is a biotin-binding protein originally isolated from Streptomyces avidinii. In contrast to avidin, streptavidin has no carbohydrate and has a mildly acidic pI of 5. Pierce streptavidin products use a recombinant form of streptavidin having a mass of 53,000 daltons and a near-neutral pI. Streptavidin is a tetrameric protein, with each subunit binding one molecule of biotin with affinity similar to that of avidin. Guanidinium chloride will dissociate avidin and streptavidin into subunits, but streptavidin is more resistant to dissociation.
Recommended for use in ELISA (0.1 µg/mL), immunohistochemistry (1-10 µg/mL), and western blot (2 µg/mL) applications.
Related products
Pierce™ Streptavidin
Pierce™ Streptavidin, Alkaline Phosphatase Conjugated
Pierce™ Streptavidin, Maleimide-Activated
Pierce™ Streptavidin, Hydrazide-Activated
Streptavidin, Horseradish Peroxidase Conjugate enables detection of biotinylated antibodies and other probes in a variety of standard assay methods, including western blotting, ELISA, immunohistochemistry, and fluorescence imaging. Streptavidin, Horseradish Peroxidase conjugate is supplied as lyophilized powder in phosphate-based buffer for immediate reconstitution with water.
Streptavidin is a biotin-binding protein originally isolated from Streptomyces avidinii. In contrast to avidin, streptavidin has no carbohydrate and has a mildly acidic pI of 5. Pierce streptavidin products use a recombinant form of streptavidin having a mass of 53,000 daltons and a near-neutral pI. Streptavidin is a tetrameric protein, with each subunit binding one molecule of biotin with affinity similar to that of avidin. Guanidinium chloride will dissociate avidin and streptavidin into subunits, but streptavidin is more resistant to dissociation.
Recommended for use in ELISA (0.1 µg/mL), immunohistochemistry (1-10 µg/mL), and western blot (2 µg/mL) applications.
Related products
Pierce™ Streptavidin
Pierce™ Streptavidin, Alkaline Phosphatase Conjugated
Pierce™ Streptavidin, Maleimide-Activated
Pierce™ Streptavidin, Hydrazide-Activated
Avidin, Texas Red™ conjugate (Invitrogen™)
Our avidin, Texas Red® conjugate is a bright, photostable probe with excitation and emission maxima ~595/615. Avidin is a glycosylated biotin-binding protein that has a basic isoelectric point and is widely used to detect biotinylated probes.
Pierce™ High Sensitivity Streptavidin-HRP (Thermo Scientific™)
Thermo Scientific Pierce High Sensitivity Streptavidin-HRP, 0.5 mL is an exclusive peroxidase-conjugated streptavidin biotin-binding protein that provides signal amplification like poly-HRP and exceptional storage stability.
Features of High Sensitivity Streptavidin-HRP, 0.5 mL:
• High sensitivity—detect low levels of target without background; obtain high signal-to-noise ratios
• Cost effective—use less conjugate in Western blotting and ELISA applications and still obtain excellent results
• Flexible—compatible with typical chemiluminescent, fluorescent and colorimetric peroxidase substrates
• Convenient—ready-to-use stabilized liquid format means there is no waiting for thawing and no need to aliquot
This specially-manufactured variety of HRP-conjugated streptavidin protein meets the demands of today's scientists for more sensitive detection in ELISA and Western blotting applications. The conjugate is suitable for use with chemiluminescent, chemifluorescent or colorimetric substrates. Each High Sensitivity HRP conjugate is packaged in an easy-to-use stabilized solution that enables convenient storage at 4°C for at least one year.
Our High Sensitivity HRP Conjugates outperform the competition on target detection, signal intensity and signal-to-noise ratios. Experiments indicate that the High Sensitivity Streptavidin-HRP can be diluted 8-to 10-fold more than traditional HRP conjugates and still produce similar results.
Related Products
Pierce™ High Sensitivity Streptavidin-HRP, Pre-Diluted
Features of High Sensitivity Streptavidin-HRP, 0.5 mL:
• High sensitivity—detect low levels of target without background; obtain high signal-to-noise ratios
• Cost effective—use less conjugate in Western blotting and ELISA applications and still obtain excellent results
• Flexible—compatible with typical chemiluminescent, fluorescent and colorimetric peroxidase substrates
• Convenient—ready-to-use stabilized liquid format means there is no waiting for thawing and no need to aliquot
This specially-manufactured variety of HRP-conjugated streptavidin protein meets the demands of today's scientists for more sensitive detection in ELISA and Western blotting applications. The conjugate is suitable for use with chemiluminescent, chemifluorescent or colorimetric substrates. Each High Sensitivity HRP conjugate is packaged in an easy-to-use stabilized solution that enables convenient storage at 4°C for at least one year.
Our High Sensitivity HRP Conjugates outperform the competition on target detection, signal intensity and signal-to-noise ratios. Experiments indicate that the High Sensitivity Streptavidin-HRP can be diluted 8-to 10-fold more than traditional HRP conjugates and still produce similar results.
Related Products
Pierce™ High Sensitivity Streptavidin-HRP, Pre-Diluted
Avidin, NeutrAvidin™, Tetramethylrhodamine conjugate (Invitrogen™)
Our tetramethylrhodamine conjugate of NeutrAvidin® biotin-binding protein - a form of avidin that has been processed to remove carbohydrate and to lower its isoelectric point - can substantially decrease background due to nonspecific binding. The method used to deglycosylate the avidin retains its specific binding. This bright, orange-fluorescent reagent (absorption/emission maxima ~555/580 nm) is ideal for detecting biotinylated targets in fluorescence microscopy applications.
