Shop All Avidin, Streptavidin & Biotin

eBioscience™ Streptavidin Super Bright 436 Conjugate (Invitrogen™)

eBioscience Streptavidin Super Bright 436 is a streptavidin-fluorochrome conjugate for indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Super Bright dyes are polymer-based fluorophores that are excited by the violet laser (405 nm) and emit fluorescence at the wavelength indicated in their name. They are optimized for use in flow cytometry and allow for use of additional channels with violet excitation. Super Bright dyes are fully compatible with other commonly used fluorescent molecules, eBioscience buffers and fixatives, viability stains, and reagents.

Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

Applications tested
Streptavidin Super Bright 436 Conjugate has been tested by flow cytometric analysis to detect biotinylated primary antibodies. The amount of this conjugate that will stain a cell sample in a final volume of 100 μL is less than or equal to 0.125 μg. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the streptavidin be carefully titrated for optimal performance in the assay of interest.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Complete Staining Buffer for more information.

Super Bright polymer dyes are sold under license from Becton, Dickinson and Company.

Streptavidin, Alexa Fluor™ 635 conjugate (Invitrogen™)

Far-red fluorescent Alexa Fluor 635 streptavidin has absorption/emission maxima of ~633/647 nm. Streptavidin is a nonglycosylated biotin-binding protein that is isolated from bacteria and has a near-neutral isoelectric point. It is widely used to detect biotinylated probes, because it reportedly exhibits less nonspecific binding than does the glycosylated, biotin-binding protein, avidin.

View a selection guide for all Alexa Fluor® streptavidin and avidin conjugates.

NeutrAvidin Protein, FITC (Invitrogen™)

NeutrAvidin for Western Blot, IHC, ELISA, ISH

pHrodo™ Red Avidin (Fluorogenic pH Sensor) (Invitrogen™)

pHrodo® Red Avidin is a conjugate of avidin and pHrodo® Red dye, a novel, fluorogenic dye that dramatically increases in fluorescence as the pH of its surroundings become more acidic. pHrodo® Red dye conjugates are non-fluorescent outside the cell, but fluoresce bright red in phagosomes, making them ideal tools for studies ranging from phagocytosis of bioparticles to receptor internalization. Original pHrodo® and pHrodo® Red dye are the same molecule.

With pHrodo® Red Avidin Fluorogenic pH Sensor You Get:
• Specific detection of phagocytosis and endocytosis
• Faster staining
• More accurate results in sensitive experiments
• Multiplexing capability with green dyes
• Validation in flow cytometry, imaging, HTS, and HCS


Get Better Results in Endocytosis Studies
Endocytosis and phagocytosis underly physiological events ranging from host-pathogen response to receptor desensitization via internalization as is common in the GPCR class of receptors). Once internalized, material can be acidified in endosomes or lysosomes. Our pHrodo® Red dye fluorogenic pH sensitive probe, when conjugated to target molecules, allows direct visual monitoring of material entering these acidic compartments. At neutral and basic pH values, pHrodo® Red dye emission is at its minimum; with acidification, pHrodo® Red dye emission is increased by as much as 8-fold (at pH 4). The red fluorescence of pHrodo® Red Avidin is ideal for multiplexing applications with green-fluorescent compounds such GFP, Fluo-4, or calcein.

Faster and More Accurate Staining in Multiple Validated Applications
pHrodo® Red Avidin offers reduced signal variability and improved timing compared to ratiometric dyes like BCECF and SNARF, and the lack of fluorescence outside the cell eliminates the need for wash steps and quencher dyes. The fluorescence of pHrodo® Red dye is dimmed by complete media and whole blood, further improving the signal-to-noise ratio. pHrodo® Red has been cited in over 150 publications and is validated in flow cytometry, high throughput screening (HTS), high content screening (HCS), and other imaging applications.

Easy Biotinylation Lets You Enjoy Flexibility in Experimental Design
The pHrodo® Red Avidin reagent can quickly be coupled to any biotinlyated reagent, including many of our own biotin conjugates of antibodies, proteins, and toxins including transferrin, EGF, and cholera toxin. Alternatively, you can create your own biotinylated target either by using Click-iT® chemistry to metabolically incorporate an alkyne biotin, or by using any of our biotin labeling kits.

Alternative Conjugation Chemistry to pHrodo® SE
In cases where the amine reactive pHrodo-SE conjugates show reduced or complete loss of function, biotin and avidin conjugation can be considered using pHrodo® Red Avidin. In one example, pHrodo-EGF was created using the avidin-biotin approach and shown to bind its receptor and be internalized (Ref 1).

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Reference:

1. Suprynowicz FA, Krawczyk E, Hebert JD, Sudarshan SR, Simic V, Kamonjoh CM, Schlegel R. The human papillomavirus type 16 E5 oncoprotein inhibits epidermal growth factor trafficking independently of endosome acidification. J Virol. 2010 Oct;84(20):10619-29. PubMed PMID: 20686024

Qdot™ 655 ITK™ Streptavidin Conjugate Kit (Invitrogen™)

The Qdot® 655 ITK™ Streptavidin Conjugate Kit provides the Qdot® streptavidin conjugate as 250 µL of a 2 µM solution. Unlike the Qdot® streptavidin conjugates, the Qdot® ITK™ streptavidin conjugates have the streptavidin covalently attached to the inner amphiphilic coating without a PEG linker. These Qdo® conjugates can be used for binding biotinylated probes. The kit also includes 30 mL of Qdot® incubation buffer.

Streptavidin, (Cy5) (Invitrogen™)

Class: RUO
Statement: For research use only. Not for use in diagnostic procedures.
Buffer: PBS
Preservative: 0.1% Sodium Azide

View a selection guide for all Alexa Fluor® streptavidin and avidin conjugates.

eBioscience™ Streptavidin APC Conjugate (Invitrogen™)

The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Streptavidin binds to biotin with high affinity.

Laser
Red Laser

Emit
660 nm

Excite
633 - 647 nm

Reported Application
Flow Cytometric Analysis

Streptavidin, Pacific Orange™ conjugate (Invitrogen™)

Pacific Orange™ streptavidin has absorption/emission maxima of ~400/551 nm. Streptavidin is a nonglycosylated biotin-binding protein that is isolated from bacteria and has a near-neutral isoelectric point. It is widely used to detect biotinylated probes, because it reportedly exhibits less nonspecific binding than does the glycosylated, biotin-binding protein, avidin.

View a selection guide for all Alexa Fluor® streptavidin and avidin conjugates.

Qdot™ 565 ITK™ Streptavidin Conjugate Kit (Invitrogen™)

The Qdot® 565 ITK™ Streptavidin Conjugate Kit provides the Qdot® streptavidin conjugate as 250 µL of a 2 µM solution. Unlike the Qdot® streptavidin conjugates, the Qdot® ITK™ streptavidin conjugates have the streptavidin covalently attached to the inner amphiphilic coating without a PEG linker. These Qdo® conjugates can be used for binding biotinylated probes. The kit also includes 30 mL of Qdot® incubation buffer.

Qdot™ 655 Streptavidin Conjugate (Invitrogen™)

Qdot® 655 streptavidin conjugate comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Qdot® nanocrystal). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays, and are also used in purification workflows to achieve targeted fractionation. Qdot® nanocrystal conjugates are provided as 1 µM solutions.

Important Features of Qdot® Streptavidin Conjugates:
Streptavidin Qdot® 655 conjugate has emission maxima of ~655 nm
Approximately 5 to 10 streptavidins per Qdot® nanocrystal
Extremely photostable and bright fluorescence
Efficiently excited with single-line excitation sources
Narrow emission, large Stokes shift
Available in multiple colors
Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more

Properties of Qdot® Nanocrystals
The Qdot® streptavidin conjugate is the size of a large macromolecule or protein (~15–20 nm) and represents the brightest class of streptavidin detection reagents. Qdot® streptavidin conjugates are made from a nanometer-scale crystal of a semiconductor material (CdSe), which is coated with an additional semiconductor shell (ZnS) to improve the optical properties of the material. The Qdot® 705 and Qdot® 800 streptavidin conjugates, which include CdSeTe, are made in a similar fashion. This core-shell material is further coated with a polymer shell that allows the materials to be conjugated to biological molecules and to retain their optical properties.

Other Fluorescent Conjugates of Streptavidin are Available
We offer several other Qdot® colors or try the Qdot® Streptavidin Sampler Kit, containing Qdot® streptavidin conjugates in six colors 525, 565, 585, 605, 655, and 705. In addition to nanocrystal conjugates, we offer a wide range of streptavidins conjugated to Alexa Fluor® dyes, Oregon Green® dye, enzyme conjugates, and traditional fluorophores like Texas Red® dye, fluorescein (FITC) and more.

Find Biotinylated Conjugates
We offer an extensive line of biotinylated conjugates for use in biotin-streptavidin detection strategies.
• Use the primary antibody search tool to find biotinylated primary antibodies
• Use the secondary antibody selector tool to find biotinylated secondary antibodies and biotinylated anti-dye and anti-hapten antibodies

Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Links:

Learn more about Avidin-Biotin Detection

To learn more about Qdot® Nanocrystals

eBioscience™ Streptavidin Super Bright 600 Conjugate (Invitrogen™)

eBioscience Streptavidin Super Bright 600 is a streptavidin-fluorochrome conjugate for indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Super Bright dyes are polymer-based fluorophores that are excited by the violet laser (405 nm) and emit fluorescence at the wavelength indicated in their name. They are optimized for use in flow cytometry and allow for use of additional channels with violet excitation. Super Bright dyes are fully compatible with other commonly used fluorescent molecules, eBioscience buffers and fixatives, viability stains, and reagents.

Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

Applications tested
Streptavidin Super Bright 600 Conjugate has been tested by flow cytometric analysis to detect biotinylated primary antibodies. The amount of this conjugate that will stain a cell sample in a final volume of 100 μL is less than or equal to 0.125 μg. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the streptavidin be carefully titrated for optimal performance in the assay of interest.

Samples can be stored in IC Fixation Buffer or 1-step Fix/Lyse Solution for up to three days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Clone specific performance after fixation should be determined empirically.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Complete Staining Buffer for more information.

Super Bright polymer dyes are sold under license from Becton, Dickinson and Company.

Streptavidin, (PE-Cy7) (Invitrogen™)

Class: RUO
Statement: For research use only. Not for use in diagnostic procedures.
Buffer: PBS
Preservative: 0.1% Sodium Azide

View a selection guide for all streptavidin RPE, APC, and tandem conjugates.

Streptavidin, Alexa Fluor™ 555 conjugate (Invitrogen™)

Alexa Fluor® 555 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor® dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays. Alexa Fluor® dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.

Important Features of Alexa Fluor® 555 Streptavidin Conjugates:
Alexa Fluor® 555 streptavidin conjugate has Ex/Em maxima of ~ (555/565)
Bright, photostable fluorescence
High solubility in aqueous solutions
Available in multiple colors
Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more

Properties of Alexa Fluor® Dyes
Alexa Fluor® dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.

Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Links:

Learn more about Avidin-Biotin Detection

Learn more about Alexa Fluor® Dyes

Find out about other Labeled Streptavidin Conjugates

Read Avidin and Streptavidin Conjugates-Section 7.6 in the Molecular Probes Handbook

CaptAvidin™ Agarose (Sedimented Bead Suspension) (Invitrogen™)

CaptAvidin™ biotin-binding protein conjugated to agarose is designed for use in affinity chromatography. CaptAvidin™ biotin-binding protein contains a nitrated tyrosine in its biotin-binding site. This chemical modification permits dissociation of the avidin-biotin complex under milder conditions than the traditional avidin-biotin complex. At pH 4.0, CaptAvidin™ biotin-binding protein binds biotin tightly with a Ka of ~109M-1. This association can then be reversed at pH 10, allowing complete dissociation of the avidin-biotin complex. This is in contrast to the harsh conditions (8 M guanidine hydrochloride, pH 1.5) needed to dissociate the avidin-biotin complex.

eBioscience™ Avidin HRP (Invitrogen™)

The avidin horseradish peroxidase is commonly used as a second step for detection of biotinylated antibodies by ELISA and western blot. Avidin binds to biotin with high affinity. [Note: Sodium azide is an inhibitor of horseradish peroxidase enzymatic activity and should not be added to the AV-HRP diluent.]

Conjugate
HRP

Reported Application
Western Blotting, ELISA