Shop All Avidin, Streptavidin & Biotin

Streptavidin, (APC) (Invitrogen™)

Class: RUO
Statement: For research use only. Not for use in diagnostic procedures.
Buffer: PBS
Preservative: 0.1% Sodium Azide

View a selection guide for all streptavidin RPE, APC, and tandem conjugates.

Streptavidin, (HRPO) (Invitrogen™)

Class: RUO
Statement: For research use only. Not for use in diagnostic procedures.
Buffer: PBS

eBioscience™ Streptavidin PerCP-Cyanine5.5 Conjugate (Invitrogen™)

The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Streptavidin binds to biotin with high affinity.

Laser
Blue Laser

Emit
695 nm

Excite
488 nm

Reported Application
Flow Cytometric Analysis

Streptavidin, (Alk. Phos.) (Invitrogen™)

Class: RUO
Statement: For research use only. Not for use in diagnostic procedures.
Buffer: PBS
Preservative: 0.1% Sodium Azide

Streptavidin, (Cy3) (Invitrogen™)

Class: RUO
Statement: For research use only. Not for use in diagnostic procedures.
Buffer: PBS
Preservative: 0.1% Sodium Azide

View a selection guide for all Alexa Fluor® streptavidin and avidin conjugates.

Streptavidin, (Cy5) (Invitrogen™)

Class: RUO
Statement: For research use only. Not for use in diagnostic procedures.
Buffer: PBS
Preservative: 0.1% Sodium Azide

View a selection guide for all Alexa Fluor® streptavidin and avidin conjugates.

Pierce™ High Sensitivity Streptavidin-HRP (Thermo Scientific™)

Thermo Scientific Pierce High Sensitivity Streptavidin-HRP, 0.5 mL is an exclusive peroxidase-conjugated streptavidin biotin-binding protein that provides signal amplification like poly-HRP and exceptional storage stability.

Features of High Sensitivity Streptavidin-HRP, 0.5 mL:

High sensitivity—detect low levels of target without background; obtain high signal-to-noise ratios
Cost effective—use less conjugate in Western blotting and ELISA applications and still obtain excellent results
Flexible—compatible with typical chemiluminescent, fluorescent and colorimetric peroxidase substrates
Convenient—ready-to-use stabilized liquid format means there is no waiting for thawing and no need to aliquot

This specially-manufactured variety of HRP-conjugated streptavidin protein meets the demands of today's scientists for more sensitive detection in ELISA and Western blotting applications. The conjugate is suitable for use with chemiluminescent, chemifluorescent or colorimetric substrates. Each High Sensitivity HRP conjugate is packaged in an easy-to-use stabilized solution that enables convenient storage at 4°C for at least one year.

Our High Sensitivity HRP Conjugates outperform the competition on target detection, signal intensity and signal-to-noise ratios. Experiments indicate that the High Sensitivity Streptavidin-HRP can be diluted 8-to 10-fold more than traditional HRP conjugates and still produce similar results.

Related Products
Pierce™ High Sensitivity Streptavidin-HRP, Pre-Diluted

eBioscience™ Streptavidin PE-Cyanine7 Conjugate (Invitrogen™)

The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Streptavidin binds to biotin with high affinity.

Laser
Blue Laser, Green Laser, Yellow-Green Laser

Emit
775 nm

Excite
488 - 561 nm

Reported Application
Flow Cytometric Analysis

Streptavidin, (PE-Cy7) (Invitrogen™)

Class: RUO
Statement: For research use only. Not for use in diagnostic procedures.
Buffer: PBS
Preservative: 0.1% Sodium Azide

View a selection guide for all streptavidin RPE, APC, and tandem conjugates.

Streptavidin, tetramethylrhodamine conjugate (Invitrogen™)

The streptavidin, tetramethylrhodamine conjugate has excitation and emission maxima ~555/580 and is widely used to detect biotinylated probes by fluorescence microscopy.

Pierce™ Streptavidin (Thermo Scientific™)

Thermo Scientific Pierce Streptavidin is a purified unconjugated recombinant protein form.

Features of Streptavidin:

Recombinant streptavidin—high-quality protein, 53kDa, pI = 6.8 to 7.5
Pure protein —choose pure, unconjugated protein for biotin blocking and controls

The purified recombinant Streptavidin enables detection of biotinylated antibodies and other probes in a variety of standard assay methods, including Western blotting, ELISA, immunohistochemistry and fluorescence imaging. Purified streptavidin is supplied as lyophilized in phosphate-based buffers for immediate reconstitution with water.

Streptavidin is biotin-binding protein that was originally isolated from Streptomyces avidinii. In contrast to avidin, streptavidin has no carbohydrate and has a mildly acidic pI of 5. Thermo Scientific Pierce Streptavidin products use a recombinant form of streptavidin having a mass of 53,000 daltons and a near-neutral pI. Streptavidin is a tetrameric protein, with each subunit binding one molecule of biotin with affinity similar to that of avidin. Guanidinium chloride will dissociate avidin and streptavidin into subunits, but streptavidin is more resistant to dissociation.

Related Products
Pierce™ Streptavidin, Horseradish Peroxidase Conjugated
Pierce™ Streptavidin, Alkaline Phosphatase Conjugated
Pierce™ Streptavidin, Maleimide-Activated
Pierce™ Streptavidin, Hydrazide-Activated

Streptavidin, Pacific Orange™ conjugate (Invitrogen™)

Pacific Orange™ streptavidin has absorption/emission maxima of ~400/551 nm. Streptavidin is a nonglycosylated biotin-binding protein that is isolated from bacteria and has a near-neutral isoelectric point. It is widely used to detect biotinylated probes, because it reportedly exhibits less nonspecific binding than does the glycosylated, biotin-binding protein, avidin.

View a selection guide for all Alexa Fluor® streptavidin and avidin conjugates.

Streptavidin Protein, PE (Invitrogen™)

Streptavidin for Western Blot, IHC, ELISA

Qdot™ 705 ITK™ Streptavidin Conjugate Kit (Invitrogen™)

The Qdot® 705 ITK™ Streptavidin Conjugate Kit provides the Qdot® streptavidin conjugate as 250 µL of a 2 µM solution. Unlike the Qdot® streptavidin conjugates, the Qdot® ITK™ streptavidin conjugates have the streptavidin covalently attached to the inner amphiphilic coating without a PEG linker. These Qdo® conjugates can be used for binding biotinylated probes. The kit also includes 30 mL of Qdot® incubation buffer.

Streptavidin Protein, AP (Invitrogen™)

Streptavidin for Western Blot, ELISA