Shop All Avidin, Streptavidin & Biotin

eBioscience™ Streptavidin eFluor™ 570 Conjugate (Invitrogen™)

The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry, immunohistochemistry, and immunocytochemistry applications. Streptavidin binds to biotin with high affinity.

Emit
570 nm

Excite
555 nm

Reported Application
Immunohistochemical Staining of Formalin-Fixed Paraffin Embedded Tissue Sections, Immunohistochemical Staining of Frozen Tissue Sections, Immunocytochemistry

Streptavidin HRP (Invitrogen™)

Purified from Streptomyces avidinii and provided in a phosphate buffered saline solution. The streptavidin/avidin-biotin staining and detection strategy is used when increased assay sensitivity is desired for a wide variety of systems.

Format/Formulation: HRP Conjugate

Pierce™ Avidin (Thermo Scientific™)

Thermo Scientific Pierce Avidin is a purified avidin protein from hen egg whites that effectively binds biotin, a tag that is frequently used to tag antibodies and other probes for immunodetection methods.

Features of Pierce Avidin:

Native avidin—glycoprotein from chicken egg whites, 67kDa, pI = 10
Soluble—glycosylation and isoelectric point renders avidin more soluble than streptavidin
Affordable—significantly less expensive than recombinant streptavidin
ABC staining—avidin is usually preferred over streptavidin for avidin-biotin complex (ABC) staining methods in immunohistochemistry (IHC)
Pure protein—choose pure, unconjugated avidin for use in ABC staining

Avidin is a glycoprotein found in the egg white and tissues of birds, reptiles and amphibia. This protein contains four identical subunits having a combined molecular mass of 67,000 to 68,000 daltons. Each subunit binds one molecule of biotin, and studies have shown that tryptophan and lysine are involved in the binding site for biotin. The sequencing of the subunit indicates it consists of 128 amino acids. Avidin has an isoelectric point of 10-10.5 and is very soluble in water and salt solutions. Avidin is stable over a wide range of pH values and temperatures. Extensive chemical modification has little effect on the activity of avidin, making it useful for detection and protein purification.

Related Products
Pierce™ Avidin, Horseradish Peroxidase Conjugated
Pierce™ Avidin, Fluorescein (FITC) Conjugated

Streptavidin R PE (Invitrogen™)

Purified from Streptomyces avidinii and provided in a phosphate buffered saline solution. The streptavidin/avidin-biotin staining and detection strategy is used when increased assay sensitivity is desired for a wide variety of systems.

Format/Formulation: RPE Conjugate

eBioscience™ Streptavidin Super Bright 780 Conjugate (Invitrogen™)

eBioscience Streptavidin Super Bright 780 is a streptavidin-fluorochrome conjugate for indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Super Bright dyes are polymer-based fluorophores that are excited by the violet laser (405 nm) and emit fluorescence at the wavelength indicated in their name. They are optimized for use in flow cytometry and allow for use of additional channels with violet excitation. Super Bright dyes are fully compatible with other commonly used fluorescent molecules, eBioscience buffers and fixatives, viability stains, and reagents.

Super Bright 780 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 780 nm. We recommend using a 780/60 bandpass filter or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

Applications tested
Streptavidin Super Bright 780 Conjugate has been tested by flow cytometric analysis to detect biotinylated primary antibodies. The amount of this conjugate that will stain a cell sample in a final volume of 100 μL is less than or equal to 0.125 μg. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the streptavidin be carefully titrated for optimal performance in the assay of interest.

Samples can be stored in IC Fixation Buffer or 1-step Fix/Lyse Solution for up to three days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Clone specific performance after fixation should be determined empirically.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Complete Staining Buffer for more information.

In some experiments, we have observed that compensation values for Super Bright 780-conjugated antibodies are higher in the violet 450/50 channel when using UltraComp eBeads microspheres as compared to single-color stained cells. In such circumstances, we would recommend setting compensation with cells. We have also observed this in some experiments using AbC Total Antibody Compensation beads.

Super Bright polymer dyes are sold under license from Becton, Dickinson and Company.

Ultra Streptavidin-HRP (Thermo Scientific™)

Streptavidin-HRP consists of streptavidin protein that is covalently conjugated to horseradish peroxidase (HRP) enzyme. Streptavidin binds to biotin and the conjugated HRP provides a high level of activity for detection using an appropriate substrate system.

Features of Streptavidin-HRP:

Product Number: N504
Size: 1.5 mL
Formulation: Conjugate in stabilizer solution containing protein and preserved with 0.02% methyliso-thiazolone, 0.02% bromonitrodioxane, and 0.002% other active isothiazolones.
Storage: Shipped on cold packs. Store at 2-8°C. Stable until expiration date if stored under these conditions.
Application: ELISA: to be used with biotin-labeled detection antibodies. Recommended dilution range is 1:400 - 1:2,000 in ELISA diluent (e.g., PBS containing 4% protease-free BSA) . The optimal dilution must be determined empirically for each specific application. Prepare dilutions immediately before use.

Streptavidin HRP (ELISA GD) (Invitrogen™)

Purified from Streptomyces avidinii and provided in a phosphate buffered saline solution. The streptavidin/avidin-biotin staining and detection strategy is used when increased assay sensitivity is desired for a wide variety of systems.

Format/Formulation: HRP Conjugate (ELISA grade)

eBioscience™ Streptavidin APC Conjugate (Invitrogen™)

The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Streptavidin binds to biotin with high affinity.

Laser
Red Laser

Emit
660 nm

Excite
633 - 647 nm

Reported Application
Flow Cytometric Analysis

Streptavidin, (R-PE) (Invitrogen™)

Class: RUO
Statement: For research use only. Not for use in diagnostic procedures.
Buffer: PBS
Preservative: 0.1% Sodium Azide

View a selection guide for all streptavidin RPE, APC, and tandem conjugates.

Avidin, NeutrAvidin™, FITC conjugate (Invitrogen™)

Searching for superior alternatives to fluorescein? Our Alexa Fluor Dye Series offers everything you're looking for and more.

Pierce™ High Sensitivity NeutrAvidin™-HRP (Thermo Scientific™)

Thermo Scientific Pierce High Sensitivity NeutrAvidin HRP Conjugate is a specially prepared peroxidase-conjugated form of avidin biotin-binding protein that provides signal amplification like poly-HRP and exceptional storage stability.

Features of High Sensitivity NeutrAvidin HRP Conjugate:

NeutrAvidin Protein—a specially deglycosylated form of avidin that provides highly specific, low-background binding of biotinylated antibodies in many applications.
High sensitivity—detect low levels of target without background; obtain high signal-to-noise ratios
Cost effective—use less conjugate in Western blotting and ELISA applications and still obtain excellent results
Flexible—compatible with typical chemiluminescent, fluorescent and colorimetric peroxidase substrates
Convenient—ready-to-use stabilized liquid format means there is no waiting for thawing and no need to aliquot

This specially-manufactured variety of HRP-conjugated avidin protein meets the demands of today's scientists for more sensitive detection in ELISA and Western blotting applications. The conjugate is suitable for use with chemiluminescent, chemifluorescent or colorimetric substrates. Each High Sensitivity HRP conjugate is packaged in an easy-to-use stabilized solution that enables convenient storage at 4°C for at least one year.

Our High Sensitivity HRP Conjugates outperform the competition on target detection, signal intensity and signal-to-noise ratios. For example, experiments indicate that the High Sensitivity Streptavidin-HRP can be diluted eight-to 10-fold more than traditional HRP conjugates and still produce similar results.

eBioscience™ Streptavidin PE Conjugate (Invitrogen™)

The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Streptavidin binds to biotin with high affinity.

Laser
Blue Laser, Green Laser, Yellow-Green Laser

Emit
578 nm

Excite
488 - 561 nm

Reported Application
Flow Cytometric Analysis

Pierce™ Avidin, Fluorescein (FITC) Conjugated (Thermo Scientific™)

Thermo Scientific Pierce FITC Conjugated Avidin is a purified avidin protein from hen egg whites that effectively binds biotin, a tag that is frequently used to tag antibodies and other probes for immunodetection methods.

Features of Pierce Avidin:

Native avidin—glycoprotein from chicken egg whites, 67kDa, pI = 10
Soluble—glycosylation and isoelectric point renders avidin more soluble than streptavidin
Affordable—significantly less expensive than recombinant streptavidin
ABC staining—avidin is usually preferred over streptavidin for avidin-biotin complex (ABC) staining methods in immunohistochemistry (IHC)
Pure protein—choose pure, unconjugated avidin for use in ABC staining

Avidin is a glycoprotein found in the egg white and tissues of birds, reptiles and amphibia. This protein contains four identical subunits having a combined molecular mass of 67,000 to 68,000 daltons. Each subunit binds one molecule of biotin, and studies have shown that tryptophan and lysine are involved in the binding site for biotin. The sequencing of the subunit indicates it consists of 128 amino acids. Avidin has an isoelectric point of 10-10.5 and is very soluble in water and salt solutions. Avidin is stable over a wide range of pH values and temperatures. Extensive chemical modification has little effect on the activity of avidin, making it useful for detection and protein purification.

Related Products
Pierce™ Avidin
Pierce™ Avidin, Horseradish Peroxidase Conjugated

Qdot™ 800 Streptavidin Conjugate (Invitrogen™)

Qdot® 800 streptavidin conjugate comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Qdot® nanocrystal). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays, and are also used in purification workflows to achieve targeted fractionation. Qdot® nanocrystal conjugates are provided as 1 µM solutions.

Important Features of Qdot® Streptavidin Conjugates:
Streptavidin Qdot® 800 conjugate has emission maxima of ~800 nm
Approximately 5 to 10 streptavidins per Qdot® nanocrystal
Extremely photostable and bright fluorescence
Efficiently excited with single-line excitation sources
Narrow emission, large Stokes shift
Available in multiple colors
Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more

Properties of Qdot® Nanocrystals
The Qdot® streptavidin conjugate is the size of a large macromolecule or protein (~15–20 nm) and represents the brightest class of streptavidin detection reagents. Qdot® streptavidin conjugates are made from a nanometer-scale crystal of a semiconductor material (CdSe), which is coated with an additional semiconductor shell (ZnS) to improve the optical properties of the material. The Qdot® 705 and Qdot® 800 streptavidin conjugates, which include CdSeTe, are made in a similar fashion. This core-shell material is further coated with a polymer shell that allows the materials to be conjugated to biological molecules and to retain their optical properties.

Other Fluorescent Conjugates of Streptavidin are Available
We offer several other Qdot® colors or try the Qdot® Streptavidin Sampler Kit, containing Qdot® streptavidin conjugates in six colors 525, 565, 585, 605, 655, and 705. In addition to nanocrystal conjugates, we offer a wide range of streptavidins conjugated to Alexa Fluor® dyes, Oregon Green® dye, enzyme conjugates, and traditional fluorophores like Texas Red® dye, fluorescein (FITC) and more.

Find Biotinylated Conjugates
We offer an extensive line of biotinylated conjugates for use in biotin-streptavidin detection strategies.
• Use the primary antibody search tool to find biotinylated primary antibodies
• Use the secondary antibody selector tool to find biotinylated secondary antibodies and biotinylated anti-dye and anti-hapten antibodies

Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Links:

Learn more about Avidin-Biotin Detection

To learn more about Qdot® Nanocrystals

Pierce™ High Sensitivity Streptavidin-HRP (Thermo Scientific™)

Thermo Scientific Pierce High Sensitivity Streptavidin-HRP, 0.5 mL is an exclusive peroxidase-conjugated streptavidin biotin-binding protein that provides signal amplification like poly-HRP and exceptional storage stability.

Features of High Sensitivity Streptavidin-HRP, 0.5 mL:

High sensitivity—detect low levels of target without background; obtain high signal-to-noise ratios
Cost effective—use less conjugate in Western blotting and ELISA applications and still obtain excellent results
Flexible—compatible with typical chemiluminescent, fluorescent and colorimetric peroxidase substrates
Convenient—ready-to-use stabilized liquid format means there is no waiting for thawing and no need to aliquot

This specially-manufactured variety of HRP-conjugated streptavidin protein meets the demands of today's scientists for more sensitive detection in ELISA and Western blotting applications. The conjugate is suitable for use with chemiluminescent, chemifluorescent or colorimetric substrates. Each High Sensitivity HRP conjugate is packaged in an easy-to-use stabilized solution that enables convenient storage at 4°C for at least one year.

Our High Sensitivity HRP Conjugates outperform the competition on target detection, signal intensity and signal-to-noise ratios. Experiments indicate that the High Sensitivity Streptavidin-HRP can be diluted 8-to 10-fold more than traditional HRP conjugates and still produce similar results.

Related Products
Pierce™ High Sensitivity Streptavidin-HRP, Pre-Diluted