Shop All Avidin, Streptavidin & Biotin

CaptAvidin™ Biotin-Binding Protein (Invitrogen™)

CaptAvidin™ biotin-binding protein contains a nitrated tyrosine in its biotin-binding site. This chemical modification permits dissociation of the avidin-biotin complex under milder conditions than the traditional avidin-biotin complex. At pH 4.0, CaptAvidin™ biotin-binding protein binds biotin tightly with a Ka of ~109M-1. This association can then be reversed at pH 10, allowing complete dissociation of the avidin-biotin complex. This is in contrast to the harsh conditions (8 M guanidine hydrochloride, pH 1.5) needed to dissociate the avidin-biotin complex.

Streptavidin, Alkaline Phosphatase Conjugate (Invitrogen™)

The streptavidin, alkaline phosphatase conjugate can be used to detect biotin in a signal amplification scheme in conjunction with chromogenic substrates or fluorogenic substrates.

Streptavidin Protein, DyLight 405 (Invitrogen™)

Streptavidin for Western Blot, IF, ICC, IHC, IP, ELISA

Streptavidin, (FITC) (Invitrogen™)

Class: RUO
Statement: For research use only. Not for use in diagnostic procedures.
Buffer: PBS
Preservative: 0.1% Sodium Azide

View a selection guide for all Alexa Fluor® streptavidin and avidin conjugates.

Qdot™ 565 ITK™ Streptavidin Conjugate Kit (Invitrogen™)

The Qdot® 565 ITK™ Streptavidin Conjugate Kit provides the Qdot® streptavidin conjugate as 250 µL of a 2 µM solution. Unlike the Qdot® streptavidin conjugates, the Qdot® ITK™ streptavidin conjugates have the streptavidin covalently attached to the inner amphiphilic coating without a PEG linker. These Qdo® conjugates can be used for binding biotinylated probes. The kit also includes 30 mL of Qdot® incubation buffer.

NeutrAvidin Protein, DyLight 633 (Invitrogen™)

NeutrAvidin for Western Blot, IF, ICC, IHC, IP, ELISA

Avidin, AP conjugate (Invitrogen™)

Reactivity: AP-Avidin reacts with biotin.

Applications: Immunohistochemistry, ELISA

Streptavidin, Alexa Fluor™ 750 conjugate (Invitrogen™)

Alexa Fluor® 750 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor® dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays. Alexa Fluor® dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.

Important Features of Alexa Fluor® 750 Streptavidin Conjugates:
Alexa Fluor® 750 streptavidin conjugate has Ex/Em maxima of ~ (749/775)
Bright, photostable fluorescence
High solubility in aqueous solutions
Available in multiple colors
Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more

Properties of Alexa Fluor® Dyes
Alexa Fluor® dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.

Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Links:

Learn more about Avidin-Biotin Detection

Learn more about Alexa Fluor® Dyes

Find out about other Labeled Streptavidin Conjugates

Read Avidin and Streptavidin Conjugates-Section 7.6 in the Molecular Probes Handbook

Streptavidin, Alexa Fluor™ 790 Conjugate (Invitrogen™)

Alexa Fluor® 790 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor® dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Alexa Fluor® dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.

Important Features of Alexa Fluor® 790 Streptavidin Conjugates:

Alexa Fluor® 790 streptavidin conjugate has Ex/Em maxima of ~(782/805)
Bright, photostable fluorescence
High solubility in aqueous solutions
Available in multiple colors
Ideal for western blots

Properties of Alexa Fluor® Dyes
Alexa Fluor® dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.

Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Links:

Learn more about Avidin-Biotin Detection

Learn more about Alexa Fluor® Dyes

Find out about other Labeled Streptavidin Conjugates

Read Avidin and Streptavidin Conjugates—Section 7.6 in the Molecular Probes Handbook

Streptavidin, Alexa Fluor™ 647—R-Phycoerythrin Conjugate (Alexa Fluor™ 647—R-Phycoerythrin Streptavidin) (Invitrogen™)

The streptavidin, Alexa Fluor™ 647-R-phycoerythrin streptavidin tandem conjugate has excitation and emission maxima at ~496,565/667 and is widely used to detect biotinylated probes by flow cytometry in multicolor applications using a single excitation wavelength, 488 nm. The Alexa Fluor™ 647 conjugates of R-PE have emission spectra almost identical to those of Cy5-R-PE conjugates but tend to have more intesne long-wavelength emission and to require less compensation in the R-PE channel.

View a selection guide for all Alexa Fluor® streptavidin and avidin conjugates.

Streptavidin, Texas Red™ conjugate (Invitrogen™)

The streptavidin, Texas Red® conjugate has excitation and emission maxima ~596/612 and is widely used to detect biotinylated probes by fluorescence microscopy.

Streptavidin, R-Phycoerythrin Conjugate (SAPE) - 1 mg/mL (Invitrogen™)

Streptavidin is a bacterially derived biotin-binding protein that exhibits less nonspecific binding than avidin. Phycoerythrin also known as R-Phycoerythrin (RPE) is an intensely bright phycobiliprotein isolated from red algae with absorption maxima at approximately 496, 546 and 565 nm, and a fluorescence emission maximum of ~578 nm. Used in flow, microplate assays and microarrays, this has remained one of our top three selling products for decades—a proven performer that you can trust.

Important Features of Streptavidin RPE
• Biotin Detection with high quantum efficiency photoprotein
• 5–10 times brighter than organic fluors
• High water solubility
• Ideal for Flow, microarrays and microplate assays
• Also available as Premium grade with higher purity confirmed by HPLC (S21388)

Streptavidin and RPE
The high affinity of avidin, an egg-white protein, for biotin was first exploited in histochemical applications in the mid-1970s. Avidin, and its bacterial counterpart, streptavidin (from Streptomy cesavidinii), have since become standard reagents for diverse detection schemes. Streptavidin, a nonglycosylated 52,800-dalton protein, exhibits less nonspecific binding than avidin.

Rhodophyta Phycoerythrin (RPE) has a MW of 240,000 Daltons and has a quantum efficiency of 78% making it an incredibly bright reagent. However, the photostability is extremely poor and is not traditionally recommended for microscopy. But the broad absorption and bright orange signal may it ideal for flow, microplate and microarray applications whenever sensitive detection of biotins is required.

Learn more about avidin biotin detection in general

Learn more about SAV-RPE

eBioscience™ Streptavidin eFluor™ 506 Conjugate (Invitrogen™)

eBioscience Streptavidin eFluor 506 is a streptavidin-fluorochrome conjugate for indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. eFluor organic dyes are traditional fluorescent dyes for use in multiple applications, including multicolor flow cytometry and immunofluorescence microscopy. To make fluorophore selection easier, all eFluor organic dyes are named after their emission wavelength, and they are fully compatible with most conventional dyes, fluorescent molecules, eBioscience buffers and fixatives, viability stains, and reagents.

The eFluor 506 organic dye is a violet-laser excitable fluorophore that has an emission peak of 506 nm. This dye is optimized for use in flow cytometry and, when combined with all five Super Bright dye antibody conjugates, can provide six detection channels of the violet laser. We recommend using a 510/20 band pass filter or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

Applications tested
Streptavidin eFluor 506 Conjugate has been tested by flow cytometric analysis to detect biotinylated primary antibodies. It can be used ≤ 0.125 μg per test. A test is defined as the amount (μg) of streptavidin fluorochrome conjugate that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the streptavidin be carefully titrated for optimal performance in the assay of interest.

Streptavidin, Alexa Fluor™ 532 conjugate (Invitrogen™)

Alexa Fluor® 532 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor® dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays. Alexa Fluor® dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.

Important Features of Alexa Fluor® 532 Streptavidin Conjugates:
Alexa Fluor® 532 streptavidin conjugate has Ex/Em maxima of ~ (530/554)
Bright, photostable fluorescence
High solubility in aqueous solutions
Available in multiple colors
Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more

Properties of Alexa Fluor® Dyes
Alexa Fluor® dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.

Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Links:

Learn more about Avidin-Biotin Detection

Learn more about Alexa Fluor® Dyes

Find out about other Labeled Streptavidin Conjugates

Read Avidin and Streptavidin Conjugates-Section 7.6 in the Molecular Probes Handbook

eBioscience™ Streptavidin eFluor™ 450 Conjugate (Invitrogen™)

The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Streptavidin binds to biotin with high affinity.

Laser
Violet Laser

Emit
445 nm

Excite
405 nm

Reported Application
Flow Cytometric Analysis

eBioscience™ Streptavidin PE-Cyanine5 Conjugate (Invitrogen™)

The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Streptavidin binds to biotin with high affinity.

Laser
Blue Laser, Green Laser, Yellow-Green Laser

Emit
667 nm

Excite
488 - 561 nm

Reported Application
Flow Cytometric Analysis

Streptavidin, (PE-TR) (Invitrogen™)

Class: RUO
Statement: For research use only. Not for use in diagnostic procedures.
Buffer: PBS
Preservative: 0.1% Sodium Azide

View a selection guide for all streptavidin RPE, APC, and tandem conjugates.

Qdot™ 545 ITK™ Streptavidin Conjugate Kit (Invitrogen™)

The Qdot® 545 ITK™ Streptavidin Conjugate Kit provides the Qdot® streptavidin conjugate as 250 µL of a 2 µM solution. Unlike the Qdot® streptavidin conjugates, the Qdot® ITK™ streptavidin conjugates have the streptavidin covalently attached to the inner amphiphilic coating without a PEG linker. These Qdo® conjugates can be used for binding biotinylated probes. The kit also includes 30 mL of Qdot® incubation buffer.

Streptavidin Protein, AP (Thermo Scientific™)

Thermo Scientific™ Streptavidin, Alkaline Phosphatase Conjugate includes streptavidin in a purified form, conjugated to alkaline phosphatase for substrate based detection.

Streptavidin is a biotin-binding protein originally isolated from Streptomyces avidinii. In contrast to avidin, streptavidin has no carbohydrate and has a mildly acidic pI of 5. Thermo Scientific streptavidin products use a recombinant form of streptavidin having a mass of 53,000 daltons and a near-neutral pI. Streptavidin is a tetrameric protein, with each subunit binding one molecule of biotin with affinity similar to that of avidin. Guanidinium chloride will dissociate avidin and streptavidin into subunits, but streptavidin is more resistant to dissociation.

Streptavidin, Alkaline Phosphatase Conjugate enables detection of biotinylated antibodies and other probes in a variety of standard assay methods, including western blotting, ELISA, immunohistochemistry, and fluorescence imaging.

Recommended concentrations: ELISA (0.1 µg/mL), immunofluorescence (assay dependent), western blot (2 µg/mL)

Streptavidin - AP (Invitrogen™)

Reactivity: AP-SA reacts with biotin.

Applications: Immunohistochemistry, Western blotting, and ELISA

eBioscience™ Streptavidin FITC Conjugate (Invitrogen™)

The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Streptavidin binds to biotin with high affinity.

Laser
Blue Laser

Emit
520 nm

Excite
488 nm

Reported Application
Flow Cytometric Analysis, Immunocytochemistry, Immunohistochemical Staining of Frozen Tissue Sections

Streptavidin, Alexa Fluor™ 660 conjugate (Invitrogen™)

Alexa Fluor® 660 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor® dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays. Alexa Fluor® dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.

Important Features of Alexa Fluor® 660 Streptavidin Conjugates:
Alexa Fluor® 660 streptavidin conjugate has Ex/Em maxima of ~ (663/690)
Bright, photostable fluorescence
High solubility in aqueous solutions
Available in multiple colors
Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more

Properties of Alexa Fluor® Dyes
Alexa Fluor® dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.

Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Links:

Learn more about Avidin-Biotin Detection

Learn more about Alexa Fluor® Dyes

Find out about other Labeled Streptavidin Conjugates

Read Avidin and Streptavidin Conjugates-Section 7.6 in the Molecular Probes Handbook

NeutrAvidin Protein, DyLight 650 (Invitrogen™)

NeutrAvidin for IF

Avidin, NeutrAvidin™, PE conjugate (Invitrogen™)

Our R-phycoerythrin (R-PE) conjugate of NeutrAvidin® biotin-binding protein - a form of avidin that has been processed to remove carbohydrate and to lower its isoelectric point - can substantially decrease background due to nonspecific binding. The method used to deglycosylate the avidin retains its specific binding. This bright, fluorescent reagent is ideal for detecting biotinylated targets in flow cytometry applications.

eBioscience™ Streptavidin APC-eFluor™ 780 Conjugate (Invitrogen™)

The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Streptavidin binds to biotin with high affinity.

Laser
Red Laser

Emit
780 nm

Excite
633 - 647 nm

Reported Application
Flow Cytometric Analysis

Qdot™ 565 Streptavidin Conjugate (Invitrogen™)

Qdot® 565 Streptavidin Conjugate comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Qdot® nanocrystal). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays, and are also used in purification workflows to achieve targeted fractionation. Qdot® nanocrystal conjugates are provided as 1 μM solutions.

Important Features of Qdot® Streptavidin Conjugates:

• Streptavidin Qdot® 565 conjugate has emission maxima of ~565 nm
• Approximately 5 to 10 streptavidins per Qdot® nanocrystal
• Extremely photostable and bright fluorescence
• Efficiently excited with single-line excitation sources
• Narrow emission, large Stokes shift
• Available in multiple colors
• Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more

Properties of Qdot® Nanocrystals
The Qdot® streptavidin conjugate is the size of a large macromolecule or protein (~15–20 nm) and represents the brightest class of streptavidin detection reagents. Qdot® streptavidin conjugates are made from a nanometer-scale crystal of a semiconductor material (CdSe), which is coated with an additional semiconductor shell (ZnS) to improve the optical properties of the material. The Qdot® 705 and Qdot® 800 streptavidin conjugates, which include CdSeTe, are made in a similar fashion. This core-shell material is further coated with a polymer shell that allows the materials to be conjugated to biological molecules and to retain their optical properties.

Other Fluorescent Conjugates of Streptavidin are Available
We offer several other Qdot® colors or try the Qdot® Streptavidin Sampler Kit, containing Qdot® streptavidin conjugates in six colors 525, 565, 585, 605, 655, and 705. In addition to nanocrystal conjugates, we offer a wide range of streptavidins conjugated to Alexa Fluor® dyes, Oregon Green® dye, enzyme conjugates, and traditional fluorophores like Texas Red® dye, fluorescein (FITC) and more.

Find Biotinylated Conjugates
We offer an extensive line of biotinylated conjugates for use in biotin-streptavidin detection strategies.

• Use the primary antibody search tool to find biotinylated primary antibodies
• Use the secondary antibody selector tool to find biotinylated secondary antibodies and biotinylated anti-dye and anti-hapten antibodies

Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.

Related Links:
Learn more about Avidin-Biotin Detection
To learn more about Qdot® Nanocrystals

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Streptavidin, Texas Red™-X conjugate (Invitrogen™)

The streptavidin, Texas Red®-X conjugate has excitation and emission maxima ~595/615 and is widely used to detect biotinylated probes by fluorescence microscopy.

Streptavidin, Alexa Fluor™ 700 conjugate (Invitrogen™)

Alexa Fluor® 700 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor® dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays. Alexa Fluor® dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.

Important Features of Alexa Fluor® 700 Streptavidin Conjugates:
Alexa Fluor® 700 streptavidin conjugate has Ex/Em maxima of ~ (702/723)
Bright, photostable fluorescence
High solubility in aqueous solutions
Available in multiple colors
Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more

Properties of Alexa Fluor® Dyes
Alexa Fluor® dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.

Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Links:

Learn more about Avidin-Biotin Detection

Learn more about Alexa Fluor® Dyes

Find out about other Labeled Streptavidin Conjugates

Read Avidin and Streptavidin Conjugates-Section 7.6 in the Molecular Probes Handbook

Streptavidin, Alexa Fluor™ 610—R-Phycoerythrin Conjugate (Alexa Fluor™ 610—R-Phycoerythrin Streptavidin) (Invitrogen™)

The streptavidin, Alexa Fluor™ 610-R-phycoerythrin streptavidin tandem conjugate has excitation and emission maxima at ~496,565/627 and is widely used to detect biotinylated probes by flow cytometry. Not only are the Alexa Fluor™ 610 conjugates of R-PE are more fluorescent than the commercially available Texas Red™ conjugates of R-PE, the fluorescent emission of Alexa Fluor™ 610-R-PE tandem conjugates is shifted to somewhat longer wavelengths than is the emission of Texas Red™-R-PE conjjgates, resulting in better separation from the emission of R-PE.

View a selection guide for all Alexa Fluor® streptavidin and avidin conjugates.

Streptavidin, tetramethylrhodamine conjugate (Invitrogen™)

The streptavidin, tetramethylrhodamine conjugate has excitation and emission maxima ~555/580 and is widely used to detect biotinylated probes by fluorescence microscopy.

eBioscience™ Streptavidin Super Bright 645 Conjugate (Invitrogen™)

eBioscience Streptavidin Super Bright 645 is a streptavidin-fluorochrome conjugate for indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Super Bright dyes are polymer-based fluorophores that are excited by the violet laser (405 nm) and emit fluorescence at the wavelength indicated in their name. They are optimized for use in flow cytometry and allow for use of additional channels with violet excitation. Super Bright dyes are fully compatible with other commonly used fluorescent molecules, eBioscience buffers and fixatives, viability stains, and reagents.

Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

Applications tested
Streptavidin Super Bright 645 Conjugate has been tested by flow cytometric analysis to detect biotinylated primary antibodies. The amount of this conjugate that will stain a cell sample in a final volume of 100 μL is less than or equal to 0.125 μg. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the streptavidin be carefully titrated for optimal performance in the assay of interest.

Samples can be stored in IC Fixation Buffer or 1-step Fix/Lyse Solution for up to three days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Clone specific performance after fixation should be determined empirically.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Complete Staining Buffer for more information.

Super Bright polymer dyes are sold under license from Becton, Dickinson and Company.

eBioscience™ Streptavidin Super Bright 436 Conjugate (Invitrogen™)

eBioscience Streptavidin Super Bright 436 is a streptavidin-fluorochrome conjugate for indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Super Bright dyes are polymer-based fluorophores that are excited by the violet laser (405 nm) and emit fluorescence at the wavelength indicated in their name. They are optimized for use in flow cytometry and allow for use of additional channels with violet excitation. Super Bright dyes are fully compatible with other commonly used fluorescent molecules, eBioscience buffers and fixatives, viability stains, and reagents.

Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

Applications tested
Streptavidin Super Bright 436 Conjugate has been tested by flow cytometric analysis to detect biotinylated primary antibodies. The amount of this conjugate that will stain a cell sample in a final volume of 100 μL is less than or equal to 0.125 μg. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the streptavidin be carefully titrated for optimal performance in the assay of interest.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Complete Staining Buffer for more information.

Super Bright polymer dyes are sold under license from Becton, Dickinson and Company.

Streptavidin Protein, DyLight 550 (Invitrogen™)

Streptavidin for IF

Streptavidin-Fluorescein Isothiocyanate (FITC) Conjugate (Invitrogen™)

Streptavidin-FITC conjugate is typically used in the immunodetection of biotinylated proteins. This conjugate is suitable for use in immunomicroscopy and fluorescence-activated cell sorting and analysis. The amount of conjugate provided is sufficient for 25 immunostaining reactions using 1 ml of working solution per reaction as appropriate.

NeutrAvidin Protein, DyLight 594 (Invitrogen™)

NeutrAvidin for Western Blot, IF, ICC, IHC, IP, ELISA

Streptavidin-CY5 (ZyMax Grade) (Invitrogen™)

Applications: Western blotting, ELISA, Immunohistochemistry, ISH

ZyMAX™ reagents offer enhanced assay sensitivity, higher titer, and greater flexibility in assay design than standard grade reagents.

Streptavidin - Agarose (Invitrogen™)

Reactivity: SA-Seph 4B reacts with biotin.

Streptavidin, Pacific Blue™ conjugate (Invitrogen™)

Our streptavidin, Pacific Blue™ conjugate is a bright, blue-fluorescent probe with excitation/emission ~410/455 nm. Streptavidin is a nonglycosylated biotin-binding protein that is isolated from bacteria and has a near-neutral isoelectric point. It is widely used to detect biotinylated probes, because it reportedly exhibits less nonspecific binding than does the glycosylated, biotin-binding protein, avidin.

View a selection guide for all Alexa Fluor® streptavidin and avidin conjugates.

eBioscience™ Streptavidin Super Bright 702 Conjugate (Invitrogen™)

eBioscience Streptavidin Super Bright 702 is a streptavidin-fluorochrome conjugate for indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Super Bright dyes are polymer-based fluorophores that are excited by the violet laser (405 nm) and emit fluorescence at the wavelength indicated in their name. They are optimized for use in flow cytometry and allow for use of additional channels with violet excitation. Super Bright dyes are fully compatible with other commonly used fluorescent molecules, eBioscience buffers and fixatives, viability stains, and reagents.

Super Bright 702 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 702 nm. We recommend using a 710/50 bandpass filter or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

Applications tested
Streptavidin Super Bright 702 Conjugate has been tested by flow cytometric analysis to detect biotinylated primary antibodies. The amount of this conjugate that will stain a cell sample in a final volume of 100 μL is less than or equal to 0.125 μg. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the streptavidin be carefully titrated for optimal performance in the assay of interest.

Samples can be stored in IC Fixation Buffer or 1-step Fix/Lyse Solution for up to three days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Clone specific performance after fixation should be determined empirically.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Complete Staining Buffer for more information.

Super Bright polymer dyes are sold under license from Becton, Dickinson and Company.

Poly-HRP Streptavidin (Thermo Scientific™)

Poly-HRP Streptavidin consists of streptavidin protein that is covalently conjugated to a polymerized form of horseradish peroxidase (HRP) enzyme. Streptavidin binds to biotin and the conjugated HRP polymer provides a high level of activity for detection using an appropriate substrate system. This particular Poly-HRP Streptavidin product has been used primarily in sandwich ELISA applications to improve the signal provided by typical (non-Poly) streptavidin-HRP conjugates.

Features of Poly-HRP Streptavidin:

Formulation: Conjugate in PBS containing distilled glycerol, BSA and a preservative.
Application: ELISA: to be used with biotin-labeled detection antibodies. Recommended dilution range is 1:2,000 - 1:20,000. The optimal dilution must be determined empirically for each specific application. Prepare dilutions immediately before use.

Related Products
Poly-HRP Dilution Buffer

Streptavidin-CY3 (ZyMax Grade) (Invitrogen™)

Applications: Immunohistochemistry, Western blotting, ELISA, ISH

ZyMAX™ reagents offer enhanced assay sensitivity, higher titer, and greater flexibility in assay design than standard grade reagents.

Streptavidin, Marina Blue™ conjugate (Invitrogen™)

Our streptavidin, Marina Blue™ conjugate is a bright, blue-fluorescent probe with excitation/emission ~365/460 nm. Streptavidin is a nonglycosylated biotin-binding protein that is isolated from bacteria and has a near-neutral isoelectric point. It is widely used to detect biotinylated probes, because it reportedly exhibits less nonspecific binding than does the glycosylated, biotin-binding protein, avidin.