Shop All Avidin & Conjugates

Avidin, FITC conjugate (Invitrogen™)

Our avidin, fluorescein conjugate has excitation and emission maxima ~494/518. Avidin is a glycosylated biotin-binding protein that has a basic isoelectric point and is widely used to detect biotinylated probes.

Avidin, Egg White (Invitrogen™)

Avidin is a glycosylated biotin-binding protein that has a basic isoelectric point and is widely used to detect biotinylated probes.

CaptAvidin™ Agarose (Sedimented Bead Suspension) (Invitrogen™)

CaptAvidin™ biotin-binding protein conjugated to agarose is designed for use in affinity chromatography. CaptAvidin™ biotin-binding protein contains a nitrated tyrosine in its biotin-binding site. This chemical modification permits dissociation of the avidin-biotin complex under milder conditions than the traditional avidin-biotin complex. At pH 4.0, CaptAvidin™ biotin-binding protein binds biotin tightly with a Ka of ~109M-1. This association can then be reversed at pH 10, allowing complete dissociation of the avidin-biotin complex. This is in contrast to the harsh conditions (8 M guanidine hydrochloride, pH 1.5) needed to dissociate the avidin-biotin complex.

Avidin, HRP conjugate (Invitrogen™)

Reactivity: HRP-Avidin reacts with biotin.

Applications: Immunohistochemistry, ELISA

Avidin, Alexa Fluor™ 488 conjugate (Invitrogen™)

Our avidin, Alexa Fluor® 488 conjugate is a bright, photostable probe with excitation and emission characteristics similar to those of fluorescein. Avidin is a glycosylated biotin-binding protein that has a basic isoelectric point and is widely used to detect biotinylated probes.

pHrodo™ Red Avidin (Fluorogenic pH Sensor) (Invitrogen™)

pHrodo® Red Avidin is a conjugate of avidin and pHrodo® Red dye, a novel, fluorogenic dye that dramatically increases in fluorescence as the pH of its surroundings become more acidic. pHrodo® Red dye conjugates are non-fluorescent outside the cell, but fluoresce bright red in phagosomes, making them ideal tools for studies ranging from phagocytosis of bioparticles to receptor internalization. Original pHrodo® and pHrodo® Red dye are the same molecule.

With pHrodo® Red Avidin Fluorogenic pH Sensor You Get:
• Specific detection of phagocytosis and endocytosis
• Faster staining
• More accurate results in sensitive experiments
• Multiplexing capability with green dyes
• Validation in flow cytometry, imaging, HTS, and HCS


Get Better Results in Endocytosis Studies
Endocytosis and phagocytosis underly physiological events ranging from host-pathogen response to receptor desensitization via internalization as is common in the GPCR class of receptors). Once internalized, material can be acidified in endosomes or lysosomes. Our pHrodo® Red dye fluorogenic pH sensitive probe, when conjugated to target molecules, allows direct visual monitoring of material entering these acidic compartments. At neutral and basic pH values, pHrodo® Red dye emission is at its minimum; with acidification, pHrodo® Red dye emission is increased by as much as 8-fold (at pH 4). The red fluorescence of pHrodo® Red Avidin is ideal for multiplexing applications with green-fluorescent compounds such GFP, Fluo-4, or calcein.

Faster and More Accurate Staining in Multiple Validated Applications
pHrodo® Red Avidin offers reduced signal variability and improved timing compared to ratiometric dyes like BCECF and SNARF, and the lack of fluorescence outside the cell eliminates the need for wash steps and quencher dyes. The fluorescence of pHrodo® Red dye is dimmed by complete media and whole blood, further improving the signal-to-noise ratio. pHrodo® Red has been cited in over 150 publications and is validated in flow cytometry, high throughput screening (HTS), high content screening (HCS), and other imaging applications.

Easy Biotinylation Lets You Enjoy Flexibility in Experimental Design
The pHrodo® Red Avidin reagent can quickly be coupled to any biotinlyated reagent, including many of our own biotin conjugates of antibodies, proteins, and toxins including transferrin, EGF, and cholera toxin. Alternatively, you can create your own biotinylated target either by using Click-iT® chemistry to metabolically incorporate an alkyne biotin, or by using any of our biotin labeling kits.

Alternative Conjugation Chemistry to pHrodo® SE
In cases where the amine reactive pHrodo-SE conjugates show reduced or complete loss of function, biotin and avidin conjugation can be considered using pHrodo® Red Avidin. In one example, pHrodo-EGF was created using the avidin-biotin approach and shown to bind its receptor and be internalized (Ref 1).

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Reference:

1. Suprynowicz FA, Krawczyk E, Hebert JD, Sudarshan SR, Simic V, Kamonjoh CM, Schlegel R. The human papillomavirus type 16 E5 oncoprotein inhibits epidermal growth factor trafficking independently of endosome acidification. J Virol. 2010 Oct;84(20):10619-29. PubMed PMID: 20686024

eBioscience™ Avidin HRP (Invitrogen™)

The avidin horseradish peroxidase is commonly used as a second step for detection of biotinylated antibodies by ELISA and western blot. Avidin binds to biotin with high affinity. [Note: Sodium azide is an inhibitor of horseradish peroxidase enzymatic activity and should not be added to the AV-HRP diluent.]

Conjugate
HRP

Reported Application
Western Blotting, ELISA

Pierce™ Avidin, Fluorescein (FITC) Conjugated (Thermo Scientific™)

Thermo Scientific Pierce FITC Conjugated Avidin is a purified avidin protein from hen egg whites that effectively binds biotin, a tag that is frequently used to tag antibodies and other probes for immunodetection methods.

Features of Pierce Avidin:

Native avidin—glycoprotein from chicken egg whites, 67kDa, pI = 10
Soluble—glycosylation and isoelectric point renders avidin more soluble than streptavidin
Affordable—significantly less expensive than recombinant streptavidin
ABC staining—avidin is usually preferred over streptavidin for avidin-biotin complex (ABC) staining methods in immunohistochemistry (IHC)
Pure protein—choose pure, unconjugated avidin for use in ABC staining

Avidin is a glycoprotein found in the egg white and tissues of birds, reptiles and amphibia. This protein contains four identical subunits having a combined molecular mass of 67,000 to 68,000 daltons. Each subunit binds one molecule of biotin, and studies have shown that tryptophan and lysine are involved in the binding site for biotin. The sequencing of the subunit indicates it consists of 128 amino acids. Avidin has an isoelectric point of 10-10.5 and is very soluble in water and salt solutions. Avidin is stable over a wide range of pH values and temperatures. Extensive chemical modification has little effect on the activity of avidin, making it useful for detection and protein purification.

Related Products
Pierce™ Avidin
Pierce™ Avidin, Horseradish Peroxidase Conjugated

Avidin, Texas Red™ conjugate (Invitrogen™)

Our avidin, Texas Red® conjugate is a bright, photostable probe with excitation and emission maxima ~595/615. Avidin is a glycosylated biotin-binding protein that has a basic isoelectric point and is widely used to detect biotinylated probes.

CaptAvidin™ Biotin-Binding Protein (Invitrogen™)

CaptAvidin™ biotin-binding protein contains a nitrated tyrosine in its biotin-binding site. This chemical modification permits dissociation of the avidin-biotin complex under milder conditions than the traditional avidin-biotin complex. At pH 4.0, CaptAvidin™ biotin-binding protein binds biotin tightly with a Ka of ~109M-1. This association can then be reversed at pH 10, allowing complete dissociation of the avidin-biotin complex. This is in contrast to the harsh conditions (8 M guanidine hydrochloride, pH 1.5) needed to dissociate the avidin-biotin complex.

Avidin, AP conjugate (Invitrogen™)

Reactivity: AP-Avidin reacts with biotin.

Applications: Immunohistochemistry, ELISA

Avidin (Invitrogen™)

Reactivity: Avidin reacts with biotin.

Pierce™ Avidin (Thermo Scientific™)

Thermo Scientific Pierce Avidin is a purified avidin protein from hen egg whites that effectively binds biotin, a tag that is frequently used to tag antibodies and other probes for immunodetection methods.

Features of Pierce Avidin:

Native avidin—glycoprotein from chicken egg whites, 67kDa, pI = 10
Soluble—glycosylation and isoelectric point renders avidin more soluble than streptavidin
Affordable—significantly less expensive than recombinant streptavidin
ABC staining—avidin is usually preferred over streptavidin for avidin-biotin complex (ABC) staining methods in immunohistochemistry (IHC)
Pure protein—choose pure, unconjugated avidin for use in ABC staining

Avidin is a glycoprotein found in the egg white and tissues of birds, reptiles and amphibia. This protein contains four identical subunits having a combined molecular mass of 67,000 to 68,000 daltons. Each subunit binds one molecule of biotin, and studies have shown that tryptophan and lysine are involved in the binding site for biotin. The sequencing of the subunit indicates it consists of 128 amino acids. Avidin has an isoelectric point of 10-10.5 and is very soluble in water and salt solutions. Avidin is stable over a wide range of pH values and temperatures. Extensive chemical modification has little effect on the activity of avidin, making it useful for detection and protein purification.

Related Products
Pierce™ Avidin, Horseradish Peroxidase Conjugated
Pierce™ Avidin, Fluorescein (FITC) Conjugated

Pierce™ Avidin, Horseradish Peroxidase Conjugated (Thermo Scientific™)

Thermo Scientific Pierce Horseradish Peroxidase Conjugated Avidin is used in biotin-based detection methods, including western blotting or ELISA detection with appropriate enzyme substrates.

Features of Avidin:

Native avidin—glycoprotein from chicken egg whites, 67kDa, pI = 10
Soluble—glycosylation and isoelectric point renders avidin more soluble than streptavidin
Affordable—significantly less expensive than recombinant streptavidin
ABC staining—avidin is usually preferred over streptavidin for avidin-biotin complex (ABC) staining methods in immunohistochemistry (IHC)
HRP conjugate—for membrane and plate-based assays

Avidin from chicken egg whites is a glycoprotein that effectively binds biotin, a tag that is frequently used to tag antibodies and other probes for immunodetection methods. HRP-conjugated avidin is useful for Western blotting or ELISA detection with appropriate enzyme substrates.

Avidin is a glycoprotein found in the egg white and tissues of birds, reptiles and amphibia. This protein contains four identical subunits having a combined molecular mass of 67,000 to 68,000 daltons. Each subunit binds one molecule of biotin, and studies have shown that tryptophan and lysine are involved in the binding site for biotin. The sequencing of the subunit indicates it consists of 128 amino acids. Avidin has an isoelectric point of 10-10.5 and is very soluble in water and salt solutions. Avidin is stable over a wide range of pH values and temperatures. Extensive chemical modification has little effect on the activity of avidin, making it useful for detection and protein purification.

Related Products
Pierce™ Avidin
Pierce™ Avidin, Fluorescein (FITC) Conjugated